CN103989731A - Acalypha australis L. and florfenicol containing compound medicine for livestock and poultry - Google Patents
Acalypha australis L. and florfenicol containing compound medicine for livestock and poultry Download PDFInfo
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- CN103989731A CN103989731A CN201410230440.6A CN201410230440A CN103989731A CN 103989731 A CN103989731 A CN 103989731A CN 201410230440 A CN201410230440 A CN 201410230440A CN 103989731 A CN103989731 A CN 103989731A
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Abstract
The invention relates to an Acalypha australis L. and florfenicol containing compound medicine for livestock and poultry. The compound medicine comprises the following raw materials in parts by weight: 3-30 parts of florfenicol and 5-200 parts of Acalypha australis L. Studies prove that after Acalypha australis L. is added to an antibacterial medicine, the resistance of bacteria to the antibacterial medicine can be reversed, thus improving the treatment effects of the antibacterial medicine. By preparing the compound medicine into powder, tablets, oral liquid or granules according to conventional methods and adding the compound medicine to feeds for livestock and poultry according to the additive amount of 0.1-7g of main medicine/kg of feed to feed the livestock and poultry, the medicine cost and the medicine residues can be substantially reduced, higher economic benefits can be created for farmers, and food safety is simultaneously ensured.
Description
Technical field
The invention belongs to poultry escherichia coli and Salmonella infection treatment technology field, relate in particular to a kind of poultry compound medicine of Guangxi precious jade medicine containing pearl grass and florfenicol that contain.
Background technology
Fowl salmonellosis (Avian salmonellosis) is the general name by the caused poultry disease of one or more Salmonellas in Salmonella.Can be divided into 3 classes according to the difference of pathogen antigen structure: Pullorum Disease, avian typhoid and avian paratyphoid.Wherein Pullorum Disease and avian typhoid Salmonella have host specificity, mainly cause chicken and turkey morbidity; Avian paratyphoid Salmonella can extensively infect many animals and people, and being subject to poultry of its pollution and products thereof is one of main source of mankind's Salmonella infection and alimentary toxicosis.The propagation circulation that the normal formation of fowl salmonellosis is quite complicated.Sick fowl and the fowl that carries disease germs are the main sources of infection, can pass through digestive tract, respiratory tract or eye conjunctiva horizontal transmission, after ovum vertical transmission, can infect poult, cause most of death, poultry carries disease germs for a long time, the ovum that then output is carried disease germs, if hatch as hatching egg using this, that can go round and begin again is handed down from age to age, and causes fowl salmonellosis in chicken group, to be difficult to purify, and causes serious economic loss.Because Salmonella pollution face in chicken group is large, and easily produce drug resistance, immune effect of vaccine is in the market all undesirable.Therefore, breeder flock quarantine, medicine control, environment disinfected purification, reinforcement feeding and management and health and epidemic prevention are still to be controlled and prevents that Salmonella from entering the essential measure of food chain.Chickling goes out to drink 0.01% potassium permanganate solution after shell or other drug carries out chemoprophylaxis for 1～2 day.This bacterium produces drug resistance to common drug, forces in production, to have to carry out various medicines and be used alternatingly, in the hope of better controlling fastbacteria.This bacterium produces stronger drug resistance to aminoglycoside medicaments at present, and aminoglycoside inactive enzyme is that the necessary group of some protection antibacterial activity in these antibacterials molecules is modified, and itself and the ribosomal affinity of target site are greatly reduced.These inactive enzymes comprise aminoglycoside acyltransferase (AAC), aminoglycoside adenylyl transferase (AAD), aminoglycoside phosphotransferase (APH), gland nucleotidyltransferase (ANT) etc.In the fastbacteria of many aminoglycosides, find at present the inactive enzyme of different qualities.
Escherichia coli are common conditioned pathogens, also be normal a kind of disease of sending out, its multiple location severe infections such as chicken acute sepsis, peritonitis, hepatitis, pneumonia, enteritis of can formerly having carried out the coffin upon burial, also can secondary or mixed sending out in virosis, causes huge economic loss to aquaculture.The beta-lactam lopps antibiotic such as amoxicillin, ampicillin, ceftiofur, ceftriaxone, amoxicillin/clavulanate, ampicillin/sulbactam are the conventional important drugs of this disease of control, along with cultivation scale intensive degree is more and more large, medication is more and more spread unchecked, its Resistant strain is more and more many, also more about the report of Drug Resistance of E. coli.Studies confirm that, one of main mechanism of drug resistance is to produce beta-lactamase, especially extended spectrumβ-lactamase (Extended-Spectrum β-lactamase, ESBLs), this enzyme can be hydrolyzed beta-lactam nucleus more by force and rapidly, makes this class antibiotic lose antibacterial activity.ESBLs is the main enzyme type of beta-lactamase, and it is not only to cephalo three generations and aztreonam drug resistance, and aminoglycoside, quinolones and sulfonamides are to crossing drug resistant, only to carbapenems, a mould alkene class medicaments insensitive.Many documents reported colibacillary multi-drug resistant with produce ESBLs have substantial connection, both at home and abroad to comparatively pay close attention to.The detection, extraction, enzyme that inventor has carried out food animal source pathogenic bacterium ESBLs recently presses down the researchs such as enzyme protection effect to antibiotic percent hydrolysis and sulbactam, Tazobactam Sodium and Chinese medicine; to part ESBLs, colibacillary drug sensitive test shows, its multidrug resistant rate is apparently higher than non-ESBLs bacterial strain.In addition, result also shows to produce ESBLs bacterial strain not only to the serious drug resistance of the third generation cephalosporins such as ceftiofur, and also to multiple antimicrobial drug drug resistance such as fluoroquinolones, aminoglycoside and sulfonamides, fosfomycin etc., is serious multidrug resistant.Inventor, producing the escherichia coli that are separated to tolerate P-levels mycin product fosA3 drug resistant gene in ESBLs bacterial strain, has more strengthened the drug resistance of antibacterial.
Salmonella and colibacillary drug resistance cause raiser's drug cost to increase, and treatment extends the course for the treatment of, and mortality of livestock increases, and brings larger economic loss to production, and increase poultry drug residue risk.Therefore, in actual production, need a kind of to no matter whether, the Salmonella of drug resistance and escherichia coli can have the medicine of better therapeutical effect badly, and can shorten and treat the course for the treatment of, reduce mortality rate, reduce economic loss and reduce drug residue, for raiser creates higher economic benefit, also ensure that the mankind's is healthy and safe.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of poultry compound medicine of Guangxi precious jade medicine containing pearl grass and florfenicol that contain, it can kill antibacterial quickly, overcome antimicrobial drug treatment due to large, the residual height of weak curative effect, consumption and the high defect of cost that drug resistance problem causes, avoided that simple Chinese medicine sterilizing spectrum is narrow, slow problem sterilizes.
For solving above technical problem, the present invention by the following technical solutions: a kind of poultry compound medicine containing containing pearl grass and florfenicol, is made up of the raw material of following weight portion: florfenicol 3-30 part, contains the careless 5-200 part of pearl.
Described compound medicine is prepared into powder, tablet, oral liquid or granule.
The preparation method of described compound medicine is: will be containing broken mistake 60 mesh sieve of pearl grass meal, then mix with pulverous antimicrobial drug, be prepared into according to a conventional method powder, tablet, oral liquid or granule.
Containing pearl grass (Acalypha australis L.), have another name called Acalypha australis, Herba Acalyphae, people Herba Amaranthi tricoloris, belong to Euphorbiaceae Acalypha, draft is to suffruticose draft, and stem is very thin, thick below 3 millimeters, female flower bract is lobate, is lobate greatly, and length can reach 1 centimetre, do not divide, there is sawtooth at edge, base portion heart, female flower is kidney shape when open, when closed, is clam, wherein keeps fruit, therefore have the reputation of " Acalypha australis ", also therefore and easily identification.Originate in various places, Guangxi, be born on the ground waste or more moistening place, limit, village.Herb decocts that water is for oral administration can treat bacillary dysentery, diarrhoea etc.
For the drug resistance problem of current poultry escherichia coli and Salmonella infection existence, inventor utilizes Chinese medicine to contain pearl grass and antimicrobial drug composition compound medicine.Studies have shown that, in antimicrobial drug, add containing after pearl grass, can reverse the drug resistance of bacteria medicine, thereby improve the therapeutic effect of antimicrobial drug.According to a conventional method this compound medicine is made to powder, tablet, oral liquid or granule, add in animal and fowl fodder and feed according to the addition of 0.1-7g principal agent/kg feedstuff, can significantly reduce drug cost and drug residue, for raiser creates higher economic benefit, also guarantee food safety simultaneously.
Detailed description of the invention
One, the drug resistance inversion effect research to antimicrobial drug containing pearl grass
Antimicrobial drug itself has the effect for the treatment of escherichia coli and Salmonella, but because escherichia coli and the Salmonella of producing drug resistant gene have stronger drug resistance, causes antimicrobial drug not show good therapeutic effect in normal using dosage situation.
1, mix with antimicrobial drug containing pearl grass after to colibacillary drug resistance inhibition test
On 96 orifice plates, adopt microtest tube doubling dilution, first measured antimicrobial drug to produce ESBLs and fosA3 drug resistant gene e. coli strains minimal inhibitory concentration and containing pearl grass to producing the subinhibitory concentration of ESBLs and fosA3 drug resistant gene e. coli strains, then in culture medium, add reusing microtest tube doubling dilution containing pearl grass and cultivating the minimal inhibitory concentration of measuring antimicrobial drug of subinhibitory concentration; Utilize continuous passage method be added with same subinhibitory concentration containing the culture medium of pearl grass and same concentration antimicrobial drug in to producing the cultivation of going down to posterity of ESBLs and fosA3 drug resistant gene e. coli strains, until the antimicrobial drug minimal inhibitory concentration observing diminishes more than 4 times compared with before going down to posterity, can be judged as containing pearl grass antibacterial medical instrument is had to drug resistance inversion effect.Result of the test statistics is as shown in table 1:
Table 1 subinhibitory concentration (0.5g/mL) goes down to posterity and causes the variation (μ g/mL) of producing ESBLs and fosA3 escherichia coli Western medicine MIC containing pearl grass.
As seen from the above table, antimicrobial drug all reduces more than 4 times the colibacillary minimal inhibitory concentration of drug resistance, can judge containing pearl grass and have drug resistance inversion effect to producing ESBLs and fosA3 drug resistant gene escherichia coli.
2, mix with antimicrobial drug containing pearl grass after drug resistance inhibition test to Salmonella
On 96 orifice plates, adopt microtest tube doubling dilution, first measure the minimal inhibitory concentration of antimicrobial drug to drug resistance salmonella strain and containing the subinhibitory concentration of pearl grass to drug resistance salmonella strain, then in culture medium, added reusing microtest tube doubling dilution containing pearl grass and cultivating the minimal inhibitory concentration of measuring antimicrobial drug of subinhibitory concentration; Utilize continuous passage method be added with same subinhibitory concentration containing the culture medium of pearl grass and same concentration antimicrobial drug in to the cultivation of going down to posterity of Salmonella persister, until the antimicrobial drug minimal inhibitory concentration observing diminishes more than 4 times compared with before going down to posterity, can be judged as containing pearl grass antibacterial medical instrument is had to drug resistance inversion effect.Result of the test statistics is as shown in table 2:
Table 2 subinhibitory concentration (0.5g/mL) goes down to posterity and causes the variation (μ g/mL) of drug resistance Salmonella Western medicine MIC containing pearl grass.
As seen from the above table, antimicrobial drug all reduces more than 4 times the minimal inhibitory concentration of drug resistance Salmonella, can judge containing pearl grass drug resistance Salmonella is had to drug resistance inversion effect.
Two, the application example of compound medicine of the present invention
Fill a prescription and following methods Preparation Example 1-embodiment 4 according to table 3: will be containing after broken mistake 60 mesh sieves of pearl grass meal, as basic material, again with pulverous antimicrobial drug by weight ratio put into blender and mix, according to a conventional method, compound medicine is prepared into formulation products.
The formula of table 3 embodiment 1-4 and preparation dosage form
| Numbering | Antimicrobial drug | Containing pearl grass | Dosage form |
| Embodiment 1 | Florfenicol 5g | 95g | Tablet |
| Embodiment 2 | Florfenicol 10g | 10g | Powder |
| Embodiment 3 | Florfenicol 12g | 150g | Oral liquid |
| Embodiment 4 | Florfenicol 30g | 180g | Granule |
1, the efficacy experiment of embodiment 1-4 gained compound medicine product to chicken escherichia coil disease
Clinical symptoms: Nanning chicken house, 25 Day-old Broiler Chickens, there is fragmentary death in chicken group, cuts open the indivedual chickens of inspection and occur the pathological change of bag pericardium liver, typical bacillus coli feature.
Laboratory diagnosis: get pathologic liver and dye sheet, Gram’s staining, micro-Microscopic observation, the gram negative bacilli of red color visible; Tentatively be judged as bacillus coli.Cultivate by escherichia coli, biochemical test is differentiated, judges that chicken infects for bacillus coli, and pcr amplification, proves to produce the ESBLs drug resistant gene that has TEM type.
Test therapeutic regimen: morbidity chicken group is divided into 6 groups of administrations, use embodiment 1 according to the addition administration of 4g/kg feedstuff for first group, use embodiment 2 according to the addition administration of 0.4g/kg feedstuff for second group, use embodiment 3 according to the addition administration of 2.8g/kg feedstuff for the 3rd group, use embodiment 4 according to the addition administration of 1.4g/kg feedstuff for the 4th group; Use florfenicol powder according to the addition administration of 0.2g/kg feedstuff for the 5th group, the 6th group with containing the thick powder of pearl grass according to the addition administration of 4g/kg feedstuff; Above six groups of therapeutic regimens all continue to use 5 days.
Effect feedback: after the chicken group medication of first group to the 4th group, sick chicken feed intake rises, and no longer occurs dead chicken, continues to use 3 days, and chicken group be almost recovered; The chicken group medication of the 5th group is after 2 days, and chicken group majority is still unhealthy, still has indivedual chicken deaths, continues to use 3 days, and chicken death phenomenon is not still effectively controlled; The chicken group medication of the 6th group is after 2 days, and chicken group does not have too large remarkable improvement, continues to use 3 days, and chicken death phenomenon is not still effectively controlled.Visible, contain containing the compound medicine curative effect of pearl grass and be better than folk prescription.
2, the efficacy experiment of embodiment 1-4 gained compound medicine product to Salmonella gallinarum disease
Clinical symptoms: Liuzhou chicken house, 12 age in days laying hens, there is dysentery in chicken group, row white, pasty state loose stool, perianal tomentum fecal pollution, has Calx sample lump, and what have sends painful shriek, has the phenomena of mortality.Cut open the indivedual Hepar Gallus domesticus enlargements of inspection and be kermesinus to darkviolet, or khaki slightly, matter is crisp easily broken, and surface is dispersed in or greyish white, the downright bad point of lark that gathers, and be red petechia sometimes, typically Salmonella genius morbi.
Laboratory diagnosis: get pathologic liver and dye sheet, Gram’s staining, micro-Microscopic observation, the gram negative bacilli of red color visible; Tentatively be judged as Salmonella disease.Cultivate by Salmonella, biochemical test is differentiated, judges that chicken infects for Salmonella disease, and drug sensitive test result is judged as persister Salmonella according to NCCLS standard.
Test therapeutic regimen: morbidity chicken group is divided into 6 groups of administrations, use embodiment 1 according to the addition administration of 4g/kg feedstuff for first group, use embodiment 2 according to the addition administration of 0.4g/kg feedstuff for second group, use embodiment 3 according to the addition administration of 2.8g/kg feedstuff for the 3rd group, use embodiment 4 according to the addition administration of 1.4g/kg feedstuff for the 4th group; Use florfenicol powder according to the addition administration of 0.2g/kg feedstuff for the 5th group, the 6th group with containing the thick powder of pearl grass according to the addition administration of 4g/kg feedstuff; Above six groups of therapeutic regimens all continue to use 5 days.
Effect feedback: after the chicken group medication of first group to the 4th group, sick chicken feed intake rises, and no longer occurs dead chicken, continues to use 3 days, and chicken group be almost recovered; The chicken group medication of the 5th group is after 2 days, and chicken group majority is still unhealthy, still has indivedual chicken deaths, continues to use 3 days, and chicken death phenomenon is not still effectively controlled; The chicken group medication of the 6th group is after 2 days, and chicken group does not have too large remarkable improvement, continues to use 3 days, and chicken death phenomenon is not still effectively controlled.Visible, contain containing the compound medicine curative effect of pearl grass and be better than folk prescription.
Claims (2)
1. contain the poultry compound medicine containing pearl grass and florfenicol, it is characterized in that, formed by the raw material of following weight portion: florfenicol 3-30 part, containing pearl grass 5-200 part.
2. the poultry compound medicine containing containing pearl grass and florfenicol according to claim 1, is characterized in that, described compound medicine is prepared into powder, tablet, oral liquid or granule.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104435012A (en) * | 2014-11-12 | 2015-03-25 | 广西大学 | Pteris multifida and florfenicol-containing compound drug for livestock and poultry |
| CN106109548A (en) * | 2016-08-22 | 2016-11-16 | 广西大学 | A kind of poultry compound medicine containing Herba Euphorbiae Humifusae and florfenicol |
| CN106138319A (en) * | 2016-08-22 | 2016-11-23 | 广西大学 | A kind of poultry compound medicine containing Fructus Rosae Laevigatae and florfenicol |
| CN106236882A (en) * | 2016-09-21 | 2016-12-21 | 广西大学 | A kind of poultry compound medicine containing Herba Euphorbiae Humifusae, Fructus Rosae Laevigatae and florfenicol |
| CN106309633A (en) * | 2016-09-21 | 2017-01-11 | 广西大学 | Livestock and poultry compound drug with Cortex Cinnamomi, Fructus Rosae Laevigatae and florfenicol |
| CN106334011A (en) * | 2016-09-21 | 2017-01-18 | 广西大学 | Liquorice, fructus rosae laevigatae and florfenicol-containing compound medicine for livestock |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104435012A (en) * | 2014-11-12 | 2015-03-25 | 广西大学 | Pteris multifida and florfenicol-containing compound drug for livestock and poultry |
| CN106109548A (en) * | 2016-08-22 | 2016-11-16 | 广西大学 | A kind of poultry compound medicine containing Herba Euphorbiae Humifusae and florfenicol |
| CN106138319A (en) * | 2016-08-22 | 2016-11-23 | 广西大学 | A kind of poultry compound medicine containing Fructus Rosae Laevigatae and florfenicol |
| CN106236882A (en) * | 2016-09-21 | 2016-12-21 | 广西大学 | A kind of poultry compound medicine containing Herba Euphorbiae Humifusae, Fructus Rosae Laevigatae and florfenicol |
| CN106309633A (en) * | 2016-09-21 | 2017-01-11 | 广西大学 | Livestock and poultry compound drug with Cortex Cinnamomi, Fructus Rosae Laevigatae and florfenicol |
| CN106334011A (en) * | 2016-09-21 | 2017-01-18 | 广西大学 | Liquorice, fructus rosae laevigatae and florfenicol-containing compound medicine for livestock |
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