CN103948656A - Creat/fosfomycin-containing compound medicine for livestock and fowl - Google Patents

Abstract

The invention relates to a creat/fosfomycin-containing compound medicine for livestock and fowl, which is prepared from the following raw materials in parts by weight: 3-30 parts of fosfomycin and 10-170 parts of creat. The raw materials are evenly mixed and prepared into powder, tablets, oral liquids or granules, and 0.1-7g of the compound medicine is added to every kilo of feed for livestock and fowl for feeding; and the feed with the compound medicine can reverse the drug resistance of bacteria to antibacterial agents, thereby enhancing the treatment effect of the antibacterial agents, greatly lowering the medicine cost and medicine residue, creating higher economic benefit for farmers and ensuring the human food safety.

Description

A kind of poultry compound medicine containing Herba Andrographis and fosfomycin

Technical field

The invention belongs to poultry coli-infection treatment technology field, relate in particular to a kind of poultry compound medicine containing Herba Andrographis and fosfomycin.

Background technology

Escherichia coli are common conditioned pathogens, also be normal a kind of disease of sending out, its multiple location severe infections such as chicken acute sepsis, peritonitis, hepatitis, pneumonia, enteritis of can formerly having carried out the coffin upon burial, also can secondary or mixed sending out in virosis, causes huge economic loss to aquaculture.The beta-lactam lopps antibiotic such as amoxicillin, ampicillin, ceftiofur, ceftriaxone, amoxicillin/clavulanate, ampicillin/sulbactam are the conventional important drugs of this disease of control, along with cultivation scale intensive degree is more and more large, medication is more and more spread unchecked, its Resistant strain is more and more many, also more about the report of Drug Resistance of E. coli.Studies confirm that, one of main mechanism of drug resistance is to produce beta-lactamase, especially extended spectrumβ-lactamase (Extended-Spectrum β-lactamase, ESBLs), this enzyme can be hydrolyzed beta-lactam nucleus more by force and rapidly, makes this class antibiotic lose antibacterial activity.ESBLs is the main enzyme type of beta-lactamase, and it is not only to cephalo three generations and aztreonam drug resistance, and aminoglycoside, quinolones and sulfonamides are to crossing drug resistant, only to carbapenems, a mould alkene class medicaments insensitive.Many documents reported colibacillary multi-drug resistant with produce ESBLs have substantial connection, both at home and abroad to comparatively pay close attention to.The detection, extraction, enzyme that inventor has carried out food animal source pathogenic bacterium ESBLs recently presses down the researchs such as enzyme protection effect to antibiotic percent hydrolysis and sulbactam, Tazobactam Sodium and Chinese medicine; to part ESBLs, colibacillary drug sensitive test shows, its multidrug resistant rate is apparently higher than non-ESBLs bacterial strain.In addition, result also shows to produce ESBLs bacterial strain not only to the serious drug resistance of the third generation cephalosporins such as ceftiofur, and also to multiple antimicrobial drug drug resistance such as fluoroquinolones, aminoglycoside and sulfonamides, fosfomycin etc., is serious multidrug resistant.Inventor, producing the escherichia coli that are separated to tolerate P-levels mycin product fosA3 drug resistant gene in ESBLs bacterial strain, has more strengthened the drug resistance of antibacterial.Colibacillary drug resistance causes raiser's drug cost to increase, and treatment extends the course for the treatment of, and mortality of livestock increases, and brings larger economic loss to production, and increases poultry drug residue risk.Therefore, in actual production, need a kind of to no matter whether, the escherichia coli of drug resistance can have the medicine of better therapeutical effect badly, and can shorten treatment course for the treatment of, reduce mortality rate, reducing economic loss and reduce drug residue, is that raiser creates higher economic benefit, also ensures that the mankind's is healthy and safe.

Summary of the invention

The technical problem to be solved in the present invention is to provide a kind of poultry compound medicine containing Herba Andrographis and fosfomycin, it can kill antibacterial quickly, overcome antimicrobial drug treatment due to large, the residual height of weak curative effect, consumption and the high defect of cost that drug resistance problem causes, avoided that simple Chinese medicine sterilizing spectrum is narrow, slow problem sterilizes.

For solving above technical problem, the present invention by the following technical solutions: a kind of poultry compound medicine containing Herba Andrographis and fosfomycin, is made up of the raw material of following weight portion: fosfomycin 3-30 part, Herba Andrographis 10-170 part.

Described compound medicine is prepared into powder, tablet, oral liquid or granule.

The preparation method of described compound medicine is: Herba Andrographis was pulverized to 60 mesh sieves, then mix with pulverous antimicrobial drug, and be prepared into according to a conventional method powder, tablet, oral liquid or granule.

The drug resistance problem existing for current poultry coli-infection, inventor utilizes Guangxi Special Traditional Chinese Medicine Herba Andrographis and antimicrobial drug composition compound medicine.Studies have shown that, in antimicrobial drug, add after Herba Andrographis, can reverse the drug resistance of bacteria medicine, thereby improve the therapeutic effect of antimicrobial drug.According to a conventional method this compound medicine is made to powder, tablet, oral liquid or granule, add in animal and fowl fodder and feed according to the addition of 0.1-7g principal agent/kg feedstuff, can significantly reduce drug cost and drug residue, for raiser creates higher economic benefit, also guarantee food safety simultaneously.

Detailed description of the invention

One, the drug resistance inversion effect research of Herba Andrographis to antimicrobial drug

Antimicrobial drug itself has the colibacillary effect for the treatment of, but because the escherichia coli that produce ESBLs drug resistant gene have stronger drug resistance, causes antimicrobial drug not show good therapeutic effect in normal using dosage situation.

In order to verify the drug resistance inversion effect of Herba Andrographis to antimicrobial drug, on 96 orifice plates, adopt microtest tube doubling dilution, first measure minimal inhibitory concentration and the subinhibitory concentration of Herba Andrographis to product ESBLs and fosA3 drug resistant gene e. coli strains of the e. coli strains of antimicrobial drug to product ESBLs and fosA3 drug resistant gene, then in culture medium, added the Herba Andrographis of subinhibitory concentration to reuse the minimal inhibitory concentration of microtest tube doubling dilution cultivation mensuration antimicrobial drug; Utilize continuous passage method in the culture medium that is added with same subinhibitory concentration Herba Andrographis and same concentration antimicrobial drug to producing the cultivation of going down to posterity of ESBLs and fosA3 drug resistant gene e. coli strains, until the antimicrobial drug minimal inhibitory concentration observing diminishes more than 4 times compared with before going down to posterity, can be judged as Herba Andrographis has drug resistance inversion effect to antibacterial medical instrument.Result of the test statistics is as shown in table 1:

Table 1 subinhibitory concentration (0.25g/mL) Herba Andrographis goes down to posterity and causes the variation of producing ESBLs and fosA3 escherichia coli Western medicine MIC.

As seen from the above table, antimicrobial drug all reduces more than 4 times the colibacillary minimal inhibitory concentration of drug resistance, can judge that Herba Andrographis has drug resistance inversion effect to producing ESBLs and fosA3 drug resistant gene escherichia coli.

Two, the application example of compound medicine of the present invention

Preparation Example 1-embodiment 4 in accordance with the following methods: Herba Andrographis was pulverized after 60 mesh sieves, as basic material, then with pulverous antimicrobial drug by weight ratio put into blender and mix, according to a conventional method, compound medicine is prepared into formulation products.

The formula of table 2 embodiment 1-4 and preparation dosage form

Numbering	Antimicrobial drug	Herba Andrographis	Dosage form
				Embodiment 1	Fosfomycin 18g	130g	Powder
Embodiment 2	Fosfomycin 3g	50g	Tablet
				Embodiment 3	Fosfomycin 9g	10g	Oral liquid
Embodiment 4	Fosfomycin 30g	170g	Granule

Inventor, by the compound medicine product of above-described embodiment 1-4 gained, carries out efficacy experiment to the meat-type duck diarrhoea of 35 ages in days.

Clinical symptoms: duck group draws watery stools, has obvious indigested feedstuff in feces.Indivedual serious duck appearance feet gradually of suffering from diarrhoea are soft slowly dead.

Cut open inspection pathological changes: the duck that dies of illness dehydration is more serious, indivedual ducks are still very large, duck liver enlargement, hyperemia, and adularescent film on the liver of part duck, air bag muddiness, tentative diagnosis is escherichia coli or duck oromeningitis.

Test therapeutic regimen: morbidity duck group divides six groups of administrations, use embodiment 1 according to the addition administration of 1g/kg feedstuff for first group, use embodiment 2 according to the addition administration of 2g/kg feedstuff for second group, use embodiment 3 according to the addition administration of 2g/kg feedstuff for the 3rd group, use embodiment 4 according to the addition administration of 1g/kg feedstuff for the 4th group; The 5th group with fosfomycin powder according to the addition administration of 0.1g/kg feedstuff, the 6th group with the thick powder of Herba Andrographis according to the addition administration of 7g/kg feedstuff; Above six groups of therapeutic regimens all continue to use 5 days.

Result of use feedback: first group to the 4th group medication is after 3 days, and sick duck is no longer drawn watery stools, feces is shaped, and no longer occurs dead duck, continues to use 2 days, and duck group be almost recovered; The 5th group of medication is after 3 days, duck group DeGrain, much still draw watery stools, contain indigested feedstuff, continue to use 2 days, some improvement a little, but thoroughly not good, rare watery stools still occurs, raiser uses the compound medicine of embodiment 1 gained instead, continue to use 3 days, feces all transfers to normally; The 6th group of medication is after 3 days, and duck does not have too large remarkable improvement, continues to use 2 days, and the duck phenomena of mortality are not still effectively controlled.Visible, the compound medicine that contains Herba Andrographis is better than folk prescription to the curative effect of duck diarrhoea.

Claims (2)

1. containing a poultry compound medicine for Herba Andrographis and fosfomycin, it is characterized in that, formed by the raw material of following weight portion: fosfomycin 3-30 part, Herba Andrographis 10-170 part.

2. the poultry compound medicine containing Herba Andrographis and fosfomycin according to claim 1, is characterized in that, described compound medicine is prepared into powder, tablet, oral liquid or granule.