CN103933544A - Creat/colimycin-containing compound medicine for livestock and fowl - Google Patents
Creat/colimycin-containing compound medicine for livestock and fowl Download PDFInfo
- Publication number
- CN103933544A CN103933544A CN201410170989.0A CN201410170989A CN103933544A CN 103933544 A CN103933544 A CN 103933544A CN 201410170989 A CN201410170989 A CN 201410170989A CN 103933544 A CN103933544 A CN 103933544A
- Authority
- CN
- China
- Prior art keywords
- compound medicine
- drug
- medicine
- herba andrographis
- fowl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
- Fodder In General (AREA)
Abstract
The invention relates to a creat/colimycin-containing compound medicine for livestock and fowl, which is prepared from the following raw materials in parts by weight: 3-30 parts of colimycin and 15-170 parts of creat. The raw materials are evenly mixed and prepared into powder, tablets, oral liquids or granules, and 0.1-7g of the compound medicine is added to every kilo of feed for livestock and fowl for feeding; and the feed with the compound medicine can reverse the drug resistance of bacteria to antibacterial agents, thereby enhancing the treatment effect of the antibacterial agents, greatly lowering the medicine cost and medicine residue, creating higher economic benefit for farmers and ensuring the human food safety.
Description
Technical field
The invention belongs to poultry coli-infection treatment technology field, relate in particular to a kind of poultry compound medicine containing Herba Andrographis and colistin.
Background technology
Escherichia coli are common conditioned pathogens, also be normal a kind of disease of sending out, its multiple location severe infections such as chicken acute sepsis, peritonitis, hepatitis, pneumonia, enteritis of can formerly having carried out the coffin upon burial, also can secondary or mixed sending out in virosis, causes huge economic loss to aquaculture.The beta-lactam lopps antibiotic such as amoxicillin, ampicillin, ceftiofur, ceftriaxone, amoxicillin/clavulanate, ampicillin/sulbactam are the conventional important drugs of this disease of control, along with cultivation scale intensive degree is more and more large, medication is more and more spread unchecked, its Resistant strain is more and more many, also more about the report of Drug Resistance of E. coli.Studies confirm that, one of main mechanism of drug resistance is to produce beta-lactamase, especially extended spectrumβ-lactamase (Extended-Spectrum β-lactamase, ESBLs), this enzyme can be hydrolyzed beta-lactam nucleus more by force and rapidly, makes this class antibiotic lose antibacterial activity.ESBLs is the main enzyme type of beta-lactamase, and it is not only to cephalo three generations and aztreonam drug resistance, and aminoglycoside, quinolones and sulfonamides are to crossing drug resistant, only to carbapenems, a mould alkene class medicaments insensitive.Many documents reported colibacillary multi-drug resistant with produce ESBLs have substantial connection, both at home and abroad to comparatively pay close attention to.The detection, extraction, enzyme that inventor has carried out food animal source pathogenic bacterium ESBLs recently presses down the researchs such as enzyme protection effect to antibiotic percent hydrolysis and sulbactam, Tazobactam Sodium and Chinese medicine; to part ESBLs, colibacillary drug sensitive test shows, its multidrug resistant rate is apparently higher than non-ESBLs bacterial strain.In addition, result also shows to produce ESBLs bacterial strain not only to the serious drug resistance of the third generation cephalosporins such as ceftiofur, and also to multiple antimicrobial drug drug resistance such as fluoroquinolones, aminoglycoside and sulfonamides, fosfomycin etc., is serious multidrug resistant.Inventor, producing the escherichia coli that are separated to tolerate P-levels mycin product fosA3 drug resistant gene in ESBLs bacterial strain, has more strengthened the drug resistance of antibacterial.Colibacillary drug resistance causes raiser's drug cost to increase, and treatment extends the course for the treatment of, and mortality of livestock increases, and brings larger economic loss to production, and increases poultry drug residue risk.Therefore, in actual production, need a kind of to no matter whether, the escherichia coli of drug resistance can have the medicine of better therapeutical effect badly, and can shorten treatment course for the treatment of, reduce mortality rate, reducing economic loss and reduce drug residue, is that raiser creates higher economic benefit, also ensures that the mankind's is healthy and safe.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of poultry compound medicine containing Herba Andrographis and colistin, it can kill antibacterial quickly, overcome antimicrobial drug treatment due to large, the residual height of weak curative effect, consumption and the high defect of cost that drug resistance problem causes, avoided that simple Chinese medicine sterilizing spectrum is narrow, slow problem sterilizes.
For solving above technical problem, the present invention by the following technical solutions: a kind of poultry compound medicine containing Herba Andrographis and colistin, is made up of the raw material of following weight portion: colistin 3-30 part, Herba Andrographis 15-170 part.
Described compound medicine is prepared into powder, tablet, oral liquid or granule.
The preparation method of described compound medicine is: Herba Andrographis was pulverized to 60 mesh sieves, then mix with pulverous antimicrobial drug, and be prepared into according to a conventional method powder, tablet, oral liquid or granule.
The drug resistance problem existing for current poultry coli-infection, inventor utilizes Guangxi Special Traditional Chinese Medicine Herba Andrographis and antimicrobial drug composition compound medicine.Studies have shown that, in antimicrobial drug, add after Herba Andrographis, can reverse the drug resistance of bacteria medicine, thereby improve the therapeutic effect of antimicrobial drug.According to a conventional method this compound medicine is made to powder, tablet, oral liquid or granule, add in animal and fowl fodder and feed according to the addition of 0.1-7g principal agent/kg feedstuff, can significantly reduce drug cost and drug residue, for raiser creates higher economic benefit, also guarantee food safety simultaneously.
Detailed description of the invention
One, the drug resistance inversion effect research of Herba Andrographis to antimicrobial drug
Antimicrobial drug itself has the colibacillary effect for the treatment of, but because the escherichia coli that produce ESBLs drug resistant gene have stronger drug resistance, causes antimicrobial drug not show good therapeutic effect in normal using dosage situation.
In order to verify the drug resistance inversion effect of Herba Andrographis to antimicrobial drug, on 96 orifice plates, adopt microtest tube doubling dilution, first measure minimal inhibitory concentration and the subinhibitory concentration of Herba Andrographis to product ESBLs and fosA3 drug resistant gene e. coli strains of the e. coli strains of antimicrobial drug to product ESBLs and fosA3 drug resistant gene, then in culture medium, added the Herba Andrographis of subinhibitory concentration to reuse the minimal inhibitory concentration of microtest tube doubling dilution cultivation mensuration antimicrobial drug; Utilize continuous passage method in the culture medium that is added with same subinhibitory concentration Herba Andrographis and same concentration antimicrobial drug to producing the cultivation of going down to posterity of ESBLs and fosA3 drug resistant gene e. coli strains, until the antimicrobial drug minimal inhibitory concentration observing diminishes more than 4 times compared with before going down to posterity, can be judged as Herba Andrographis has drug resistance inversion effect to antibacterial medical instrument.Result of the test statistics is as shown in table 1:
Table 1 subinhibitory concentration (0.25g/mL) Herba Andrographis goes down to posterity and causes the variation (mg/mL) of producing ESBLs and fosA3 escherichia coli Western medicine MIC.
As seen from the above table, antimicrobial drug all reduces more than 4 times the colibacillary minimal inhibitory concentration of drug resistance, can judge that Herba Andrographis has drug resistance inversion effect to producing ESBLs and fosA3 drug resistant gene escherichia coli.
Two, the application example of compound medicine of the present invention
Preparation Example 1-embodiment 4 in accordance with the following methods: Herba Andrographis was pulverized after 60 mesh sieves, as basic material, then with pulverous antimicrobial drug by weight ratio put into blender and mix, according to a conventional method, compound medicine is prepared into formulation products.
The formula of table 2 embodiment 1-4 and preparation dosage form
| Numbering | Antimicrobial drug | Herba Andrographis | Dosage form |
| Embodiment 1 | Colistin 10g | 170g | Powder |
| Embodiment 2 | Colistin 3g | 60g | Tablet |
| Embodiment 3 | Colistin 18g | 15g | Oral liquid |
| Embodiment 4 | Colistin 30g | 120g | Granule |
Inventor, by the compound medicine product of above-described embodiment 1-4 gained, on Guangxi Lin pig farm, carries out efficacy experiment to the piglet diarrhea of 40 ages in days.
Clinical symptoms:, this swinery after wean is turn-taked, starts to occur diarrhoea, draws watery stools, feces contains indigested feedstuff, pig material feeding but be exactly not grow, and indivedual serious diarrhea of pigs dehydrations are dead, do not occur symptoms of emesis, raiser uses multi-medicament, and ideal effect does not all appear in treatment.
Cut open inspection pathological changes: after cuing open inspection, Intestinum Sus domestica wall is poor, especially small intestinal rear end and colon position, intestinal mucosa comes off, intestinal wall hyperemia, hemorrhage.
Laboratory diagnosis: get pathologic liver and dye sheet, Gram’s staining, micro-Microscopic observation, the gram negative bacilli of red color visible; Tentatively be judged as bacillus coli.Cultivate by escherichia coli, biochemical test is differentiated, judges that Infection in Piglets is bacillus coli, and pcr amplification proves to produce the ESBLs drug resistant gene and the fosA3 drug resistant gene that have CTX-M type.
Test therapeutic regimen: morbidity swinery is divided 6 groups of administrations, use embodiment 1 according to the addition administration of 2g/kg feedstuff for first group, use embodiment 2 according to the addition administration of 2g/kg feedstuff for second group, use embodiment 3 according to the addition administration of 0.3g/kg feedstuff for the 3rd group, use embodiment 4 according to the addition administration of 0.4g/kg feedstuff for the 4th group; The 5th group with colistin powder according to the addition administration of 0.1g/kg feedstuff, the 6th group with the thick powder of Herba Andrographis according to the addition administration of 7g/kg feedstuff; Above six groups of therapeutic regimens all continue to use 5 days.
Result of use feedback: first group to the 4th group medication is after 2 days, and swinery is no longer drawn watery stools, feces is shaped, and no longer contains feedstuff in feces, continues to use 3 days swinery recovery from illness; The 5th group of medication is after 2 days, and swinery is still unhealthy, continues to use 3 days, substantially get well, but still draw pasty state feces individually, but drug withdrawal is after 3 days, and recurrence appears in swinery; The 6th group of medication is after 2 days, and swinery, without remarkable improvement, continues to use 3 days, and swinery continues to have loose bowels, and all the other are also more serious individually.Visible, the compound medicine that contains Herba Andrographis is better than folk prescription to swinery curative effect.
Claims (2)
1. containing a poultry compound medicine for Herba Andrographis and colistin, it is characterized in that, formed by the raw material of following weight portion: colistin 3-30 part, Herba Andrographis 15-170 part.
2. the poultry compound medicine containing Herba Andrographis and colistin according to claim 1, is characterized in that, described compound medicine is prepared into powder, tablet, oral liquid or granule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410170989.0A CN103933544B (en) | 2014-04-25 | 2014-04-25 | A kind of poultry compound medicine containing Herba Andrographis and colistin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410170989.0A CN103933544B (en) | 2014-04-25 | 2014-04-25 | A kind of poultry compound medicine containing Herba Andrographis and colistin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103933544A true CN103933544A (en) | 2014-07-23 |
| CN103933544B CN103933544B (en) | 2015-11-25 |
Family
ID=51181599
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410170989.0A Expired - Fee Related CN103933544B (en) | 2014-04-25 | 2014-04-25 | A kind of poultry compound medicine containing Herba Andrographis and colistin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103933544B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104436160A (en) * | 2014-11-07 | 2015-03-25 | 广西大学 | Balsamiferou blumea herb and colistin containing compound medicine for livestock and poultry |
| CN104436161A (en) * | 2014-11-12 | 2015-03-25 | 中国农业科学院哈尔滨兽医研究所 | Pteris multifida and colistin-containing compound drug for livestock and poultry |
| CN105343160A (en) * | 2015-12-11 | 2016-02-24 | 广西南宁市桃源兽药厂 | Compound composition resisting livestock and poultry escherichia coli |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102058638A (en) * | 2011-01-07 | 2011-05-18 | 河南亚卫动物药业有限公司 | Compound andrographis paniculata injection for treating yellow-white dysentery of piglet and preparation method thereof |
| CN102813910A (en) * | 2012-08-09 | 2012-12-12 | 南京农业大学 | Medicine composition used for controlling poultry airsacculitis, and preparation method thereof |
| CN102813909A (en) * | 2012-08-02 | 2012-12-12 | 青岛绿曼生物工程有限公司 | Compound doxycycline hydrochloride composition for preventing and treating colibacillosis and chronic respiratory disease of poultry as well as preparation method thereof |
| CN103182018A (en) * | 2013-03-21 | 2013-07-03 | 河北科技师范学院 | Chinese herbal medicine feed additive for treating chicken colibacillosis and chicken salmonellosis |
-
2014
- 2014-04-25 CN CN201410170989.0A patent/CN103933544B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102058638A (en) * | 2011-01-07 | 2011-05-18 | 河南亚卫动物药业有限公司 | Compound andrographis paniculata injection for treating yellow-white dysentery of piglet and preparation method thereof |
| CN102813909A (en) * | 2012-08-02 | 2012-12-12 | 青岛绿曼生物工程有限公司 | Compound doxycycline hydrochloride composition for preventing and treating colibacillosis and chronic respiratory disease of poultry as well as preparation method thereof |
| CN102813910A (en) * | 2012-08-09 | 2012-12-12 | 南京农业大学 | Medicine composition used for controlling poultry airsacculitis, and preparation method thereof |
| CN103182018A (en) * | 2013-03-21 | 2013-07-03 | 河北科技师范学院 | Chinese herbal medicine feed additive for treating chicken colibacillosis and chicken salmonellosis |
Non-Patent Citations (1)
| Title |
|---|
| 张国祖 等: "穿心莲水提物与10 种临床常用抗菌药联用的体外抑菌试验", 《中国畜牧兽医》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104436160A (en) * | 2014-11-07 | 2015-03-25 | 广西大学 | Balsamiferou blumea herb and colistin containing compound medicine for livestock and poultry |
| CN104436161A (en) * | 2014-11-12 | 2015-03-25 | 中国农业科学院哈尔滨兽医研究所 | Pteris multifida and colistin-containing compound drug for livestock and poultry |
| CN105343160A (en) * | 2015-12-11 | 2016-02-24 | 广西南宁市桃源兽药厂 | Compound composition resisting livestock and poultry escherichia coli |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103933544B (en) | 2015-11-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103690952B (en) | The compound medicine for the treatment of poultry coli-infection disease | |
| CN103933086A (en) | Creat/amikacin-containing compound medicine for livestock and fowl | |
| CN103961421A (en) | Compound medicine containing radix paeoniae rubra and mequindox for livestock and poultry | |
| CN103127508B (en) | Herba lycopi containing compound composition for treating escherichia coli infected diseases of livestock and poultry | |
| CN103961400A (en) | Compound medicine comprising herba lycopi and florfenicol and used for livestock and poultry | |
| CN103948656A (en) | Creat/fosfomycin-containing compound medicine for livestock and fowl | |
| CN103977088A (en) | Compound medicine containing radix paeoniae rubrathe and fluoroquinolones antibacterial drug used for livestock and poultry | |
| CN104306420A (en) | Balsamiferou blumea herb and sulfamonomethoxine sodium containing compound medicine for livestock and poultry | |
| CN103948692A (en) | Red paeonia/amikacin-containing compound medicine for livestock and fowl | |
| CN103301463A (en) | Compound composition containing plumbago zeylanica for treating livestock and poultry escherichia coli infection diseases | |
| CN103933544B (en) | A kind of poultry compound medicine containing Herba Andrographis and colistin | |
| CN103933543B (en) | Utilize the method for Herba Andrographis and colistin treatment poultry coli-infection disease | |
| CN103948677A (en) | Boneset/amoxicillin-containing compound medicine for livestock and fowl | |
| CN104027369A (en) | Plumbago zeylanica and norfloxacin containing compound composition for livestock and poultry | |
| CN104436160A (en) | Balsamiferou blumea herb and colistin containing compound medicine for livestock and poultry | |
| CN103908670B (en) | A kind of compound treating poultry coli-infection disease | |
| CN103961401A (en) | Compound medicine comprising herba lycopi and sulfamonomethoxine sodium and used for livestock and poultry | |
| CN104367601A (en) | Compound medicine containing herba pteridis multifidae and mequindox for livestock and poultry | |
| CN104337847A (en) | Compound medicine containing bracken and fosfomycin for livestock | |
| CN104435038A (en) | Balsamiferou blumea herb and mequindox containing compound medicine for livestock and poultry | |
| CN103948912B (en) | A kind of livestock and poultry compound medicine containing the radix paeoniae rubrathe and colistin | |
| CN104435013A (en) | Pteris multifida and amikacin-containing compound drug for livestock and poultry | |
| CN104027370A (en) | Plumbago zeylanica and mequindox containing compound composition for livestock and poultry | |
| CN104436161A (en) | Pteris multifida and colistin-containing compound drug for livestock and poultry | |
| CN103933087A (en) | Method for treating Escherichia coli infection diseases by using creat and amikacin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160401 Address after: 276817 Industrial Park, Nanhu Town, Donggang District, Shandong, Rizhao City Patentee after: Shandong Botai Biological Pharmaceutical Co Ltd Address before: 530004 the Guangxi Zhuang Autonomous Region XiXiangTang Nanning University Road No. 100 Patentee before: Guangxi University |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151125 Termination date: 20160425 |