CN103948912B - A kind of livestock and poultry compound medicine containing the radix paeoniae rubrathe and colistin - Google Patents
A kind of livestock and poultry compound medicine containing the radix paeoniae rubrathe and colistin Download PDFInfo
- Publication number
- CN103948912B CN103948912B CN201410202847.8A CN201410202847A CN103948912B CN 103948912 B CN103948912 B CN 103948912B CN 201410202847 A CN201410202847 A CN 201410202847A CN 103948912 B CN103948912 B CN 103948912B
- Authority
- CN
- China
- Prior art keywords
- radix paeoniae
- paeoniae rubrathe
- antimicrobial
- colistin
- compound medicine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Fodder In General (AREA)
Abstract
The present invention relates to a kind of livestock and poultry compound medicine containing the radix paeoniae rubrathe and colistin, be made up of the raw material of following weight portion: colistin 3 30 parts, the radix paeoniae rubrathe 5 200 parts.Inventor utilizes the radix paeoniae rubrathe to form compound medicine with antimicrobial.Research proves, after adding the radix paeoniae rubrathe, it is possible to the reverse bacterial drug resistance to antimicrobial, thus improves the result for the treatment of of antimicrobial in antimicrobial.According to a conventional method this compound medicine is made powder, tablet, oral liquid or granule, add in animal and fowl fodder according to the addition of 0.1 7g main ingredient/kg feed and feed, drug cost and medicament residue can be greatly reduced, create higher economic benefit for raiser, also ensure that food security simultaneously.
Description
Technical field
The invention belongs to livestock and poultry coli-infection treatment technology field, particularly relate to a kind of containing the radix paeoniae rubrathe with the livestock and poultry of colistin
Use compound medicine.
Background technology
Escherichia coli are common conditioned pathogens, are also a kind of diseases often sent out, and it primary can cause chicken acute sepsis, abdomen
The multiple location severe infections such as film inflammation, hepatitis, pneumonia, enteritis, also can secondary or mixed send out in virosis, cause huge to aquaculture
Economic loss.Amoxicillin, ampicillin, Ceftiofur, ceftriaxone, amoxicillin/clavulanate, ampicillin/
The beta-lactam nucleus class antibiotic such as Sulbactam are the conventional important drugs preventing and treating this disease, along with cultivation scale intensive degree more comes
Bigger, medication is more and more spread unchecked, and its antibody-resistant bacterium is the most, and the report about Drug Resistance of E. coli is the most more.Research card
Real, one of main mechanism of resistance is to produce beta-lactamase, especially extended spectrumβ-lactamase (Extended-
Spectrum β-lactamase, ESBLs), this enzyme can hydrolyze beta-lactam nucleus more by force and rapidly, makes this kind of antibiotic lose
Lose antibacterial activity.ESBLs is the main enzyme type of beta-lactamase, and it is not only to cephalo three generations and AZT resistance, and right
Aminoglycoside, quinolones and sulfamido are crossing drug resistant, only to Carbapenems, a mould alkenes medicaments insensitive.Many literary compositions
Offer it has been reported that colibacillary multi-drug resistant with produce ESBLs have substantial connection, both at home and abroad to more pay close attention to.Inventor
The detection of food animal source pathogenic bacteria ESBLs, extraction, enzyme are carried out recently to the percent hydrolysis of antibiotic and Sulbactam, his azoles bar
Smooth and Chinese medicine the enzyme protection effect that presses down etc. is studied, and drug sensitive test colibacillary to part ESBLs shows, its multidrug resistant rate
Apparently higher than non-ESBLs bacterial strain.It addition, result also shows to produce ESBLs bacterial strain not only to third generation cephalosporins such as Ceftiofurs
Serious resistance, and also antimicrobial resistance multiple to FQNS, aminoglycoside and sulfamido, phosphonomycin etc., in seriously
Multidrug resistant.Inventor is separated to resistance to phosphonomycin in producing ESBLs bacterial strain and produces the Escherichia coli of fosA3 drug resistant gene, more
The strong drug resistance of bacterium.Colibacillary drug resistance causes raiser's drug cost to increase, and treatment extends the course for the treatment of, and livestock and poultry are dead
Rate increases, and brings bigger economic loss to production, and increases livestock and poultry medicament residue risk.Therefore, actual production needs one badly
Kind to regardless of whether the Escherichia coli of resistance can have a medicine of preferable therapeutic action, and can shorten and treat the course for the treatment of, the minimizing death rate,
Reduce economic loss and reduce medicament residue, creating higher economic benefit for raiser, also ensure that the mankind's is healthy and safe.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of livestock and poultry compound medicine containing the radix paeoniae rubrathe and colistin, and it can be relatively
Kill bacterium soon, overcome weak curative effect that antimicrobial treatment causes due to drug resistance problems, consumption is big, residual is high and cost is high
Defect, it is to avoid simple Chinese medicine sterilizing spectrum is narrow, be sterilized slow problem.
For solving above technical problem, the present invention by the following technical solutions: a kind of livestock and poultry containing the radix paeoniae rubrathe and colistin are with multiple
Side's medicine, is made up of the raw material of following weight portion: colistin 3-30 part, radix paeoniae rubrathe 5-200 part.
Described compound medicine is prepared as powder, tablet, oral liquid or granule.
The preparation method of described compound medicine is: the radix paeoniae rubrathe pulverized 60 mesh sieves, then mixes, by often with the antimicrobial of powder
Rule method is prepared as powder, tablet, oral liquid or granule.
The drug resistance problems existed for current livestock and poultry coli-infection, inventor utilizes the radix paeoniae rubrathe to form compound medicine with antimicrobial.
Research proves, after adding the radix paeoniae rubrathe, it is possible to the reverse bacterial drug resistance to antimicrobial, thus improves the treatment of antimicrobial in antimicrobial
Effect.According to a conventional method this compound medicine is made powder, tablet, oral liquid or granule, raise according to 0.1-7g main ingredient/kg
The addition of material adds in animal and fowl fodder and feeds, and drug cost and medicament residue can be greatly reduced, and creates more for raiser
High economic benefit, also ensure that food security simultaneously.
Detailed description of the invention
One, the radix paeoniae rubrathe drug resistance inversion Effect study to antimicrobial
Antimicrobial itself has the colibacillary effect for the treatment of, but has stronger owing to producing the Escherichia coli of ESBLs drug resistant gene
Drug resistance, causes antimicrobial not show preferable result for the treatment of in the case of normal dosage.
In order to verify the radix paeoniae rubrathe drug resistance inversion effect to antimicrobial, 96 orifice plates use microtest tube doubling dilution, first measures
Antimicrobial to produce the minimal inhibitory concentration of e. coli strains of ESBLs and fosA3 drug resistant gene and the radix paeoniae rubrathe to produce ESBLs and
The subinhibitory concentration of fosA3 drug resistant gene e. coli strains, the radix paeoniae rubrathe adding subinhibitory concentration the most in the medium reuses
Microtest tube doubling dilution cultivates the minimal inhibitory concentration measuring antimicrobial；Utilize continuous passage method added with same sub-antibacterial dense
The culture medium of the degree radix paeoniae rubrathe and same concentration antimicrobial carries out Secondary Culture to product ESBLs and fosA3 drug resistant gene e. coli strains,
Until the antimicrobial minimal inhibitory concentration observed with pass on before compared with diminish more than 4 times, can be judged as that the radix paeoniae rubrathe is to antibacterial medical instrument
There is drug resistance inversion effect.Result of the test statistics is as shown in table 1:
Table 1 subinhibitory concentration (0.5g/mL) radix paeoniae rubrathe passes on and causes the change producing ESBLs and fosA3 Escherichia coli Western medicine MIC
(mg/mL)。
As seen from the above table, antimicrobial all reduces more than 4 times to the minimal inhibitory concentration of antibiotic-resistance E. coli, i.e. can determine whether it is the radix paeoniae rubrathe
To producing ESBLs and fosA3 drug resistant gene Escherichia coli, there is drug resistance inversion effect.
Two, the application example of compound medicine of the present invention
According to table 2 formula and following methods prepare embodiment 1-embodiment 6: after the radix paeoniae rubrathe was pulverized 60 mesh sieves, based on former
Material, then mix during ratio puts into blender by weight with the antimicrobial of powder, according to a conventional method, compound medicine is prepared as
Formulation products.
The formula of table 2 embodiment 1-6 and preparation formulation
| Numbering | Antimicrobial | The radix paeoniae rubrathe | Formulation |
| Embodiment 1 | Colistin 10g | 170g | Powder |
| Embodiment 2 | Colistin 3g | 60g | Tablet |
| Embodiment 3 | Colistin 18g | 15g | Oral liquid |
| Embodiment 4 | Colistin 30g | 120g | Granule |
| Embodiment 5 | Colistin 3g | 5g | Powder |
| Embodiment 6 | Colistin 30g | 200g | Tablet |
Inventor is by the compound medicine product of above-described embodiment 1-6 gained, the piglet abdomen on pig farm, Nanning, to 38 ages in days
Rush down and carry out efficacy experiment.
Clinical symptoms: this swinery, after wean is turn-taked, starts diarrhoea occur, draws watery stools, ight soil to contain indigested feed,
Pig material feeding but be exactly the longest, the most serious diarrhea of pigs dehydration death, symptoms of emesis does not occur, raiser uses multi-medicament,
The most there is not ideal effect in treatment.
Cuing open inspection pathology: after cuing open inspection, chitling wall is belittled, especially small intestine rear end and colon site, and intestinal mucosa comes off, intestinal wall is congested, go out
Blood.
Laboratory diagnosis: take pathologic liver dye sheet, Gram's staining, basis of microscopic observation, it is seen that red Gram-negative bar
Bacterium；Tentatively it is judged as bacillus coli.Being cultivated by Escherichia coli, biochemical test differentiates, it is judged that Infection in Piglets is large intestine bar
Bacterial diseases, PCR expands, it was demonstrated that produce ESBLs drug resistant gene and the fosA3 drug resistant gene having CTX-M type.
Test therapeutic regimen: morbidity swinery divides 8 groups of administrations, and first group is administered according to the addition of 2g/kg feed by embodiment 1,
Second group is administered according to the addition of 2g/kg feed by embodiment 2, and the 3rd group with embodiment 3 adding according to 0.3g/kg feed
Dosage is administered, and the 4th group is administered according to the addition of 0.4g/kg feed by embodiment 4；5th group with embodiment 5 according to
The addition of 0.25g/kg feed is administered, and the 6th group is administered according to the addition of 0.75g/kg feed by embodiment 6；7th group
Being administered according to the addition of 0.1g/kg feed with colistin pulvis, the 8th group with the radix paeoniae rubrathe thick powder adding according to 4g/kg feed
Dosage is administered；Above eight groups of therapeutic regimens the most persistently use 5 days.
Using effect is fed back: after first group to the 6th group medication 2 days, swinery no longer draws watery stools, and formed stools, in ight soil
No longer containing feed, be continuing with 3 days, swinery is fully recovered；After 7th group of medication 2 days, swinery is the most unhealthy, is continuing with 3
My god, substantially get well, but still draw individually pasty state ight soil, but after drug withdrawal 3 days, recurrence occurs in swinery；8th group of medication 2
After it, swinery, without remarkable improvement, is continuing with 3 days, and swinery continues to have loose bowels, and remaining the most also ratio is more serious.Visible, contain
The compound medicine of the radix paeoniae rubrathe is better than folk prescription to swinery curative effect.
Claims (2)
1. the radix paeoniae rubrathe and colistin are combined in preparation application in terms for the treatment of livestock and poultry coli-infection disease preparation, its feature
Being, in described preparation, the weight ratio of the radix paeoniae rubrathe and colistin is: colistin 3-30 part, radix paeoniae rubrathe 5-200 part.
Application the most according to claim 1, it is characterised in that described preparation is powder, tablet, oral liquid or particle
Agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410202847.8A CN103948912B (en) | 2014-05-14 | 2014-05-14 | A kind of livestock and poultry compound medicine containing the radix paeoniae rubrathe and colistin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410202847.8A CN103948912B (en) | 2014-05-14 | 2014-05-14 | A kind of livestock and poultry compound medicine containing the radix paeoniae rubrathe and colistin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103948912A CN103948912A (en) | 2014-07-30 |
| CN103948912B true CN103948912B (en) | 2016-09-07 |
Family
ID=51326352
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410202847.8A Active CN103948912B (en) | 2014-05-14 | 2014-05-14 | A kind of livestock and poultry compound medicine containing the radix paeoniae rubrathe and colistin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103948912B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104840942A (en) * | 2015-01-09 | 2015-08-19 | 广西南宁市桃源兽药厂 | Composition containing epimedium herb and colistin and used for livestock and poultry, and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101972392A (en) * | 2010-08-25 | 2011-02-16 | 天津生机集团股份有限公司 | Chinese medicinal composition for treating chicken colibacillosis and preparation method thereof |
| CN103301463A (en) * | 2013-06-13 | 2013-09-18 | 广西南宁市桃源兽药厂 | Compound composition containing plumbago zeylanica for treating livestock and poultry escherichia coli infection diseases |
-
2014
- 2014-05-14 CN CN201410202847.8A patent/CN103948912B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101972392A (en) * | 2010-08-25 | 2011-02-16 | 天津生机集团股份有限公司 | Chinese medicinal composition for treating chicken colibacillosis and preparation method thereof |
| CN103301463A (en) * | 2013-06-13 | 2013-09-18 | 广西南宁市桃源兽药厂 | Compound composition containing plumbago zeylanica for treating livestock and poultry escherichia coli infection diseases |
Non-Patent Citations (4)
| Title |
|---|
| 20种中草药对多重耐药大肠杆菌的体外抑菌作用;孙喆等;《吉林畜牧兽医》;20091231;第30卷(第11期);6-8 * |
| In vitro efficacy of bioactive extracts of 15 medicinal plants against ESbL-producing multidrug-resistant enteric bacteria;Iqbal Ahmad等;《Microbiological Research》;20071231;第162卷;264-275页 * |
| 禽大肠杆菌ESBLs动力学及中药提取物对其抑制作用的研究;石科;《中国优秀硕士学位论文全文数据库 农业科技辑》;20090415(第4期);D050-91 * |
| 赤芍提取物体外抑菌活性的研究;张芳等;《徐州医学院学报》;20061231;第26卷(第5期);摘要部分，第419页第2.1部分至第420页左栏最后一段 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103948912A (en) | 2014-07-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103690952B (en) | The compound medicine for the treatment of poultry coli-infection disease | |
| CN103989728B (en) | It is a kind of to contain the livestock and poultry compound medicine containing pearl grass and beta-lactam class antimicrobial | |
| CN103933086A (en) | Creat/amikacin-containing compound medicine for livestock and fowl | |
| CN103977089B (en) | A kind of poultry compound medicine containing Radix Paeoniae Rubra and fosfomycin | |
| CN103977088B (en) | A kind of poultry compound medicine containing Radix Paeoniae Rubra and fluoroquinolone antibacterial agent | |
| CN103948656B (en) | A kind of poultry compound medicine containing Herba Andrographis and fosfomycin | |
| CN103948691B (en) | A kind of poultry compound medicine containing Radix Paeoniae Rubra and sulfamonomethoxine sodium | |
| CN103948692B (en) | A kind of livestock and poultry compound medicine containing the radix paeoniae rubrathe and amikacin | |
| CN104306420A (en) | Balsamiferou blumea herb and sulfamonomethoxine sodium containing compound medicine for livestock and poultry | |
| CN103933544B (en) | A kind of poultry compound medicine containing Herba Andrographis and colistin | |
| CN103933543B (en) | Utilize the method for Herba Andrographis and colistin treatment poultry coli-infection disease | |
| CN103908670B (en) | A kind of compound treating poultry coli-infection disease | |
| CN104436160A (en) | Balsamiferou blumea herb and colistin containing compound medicine for livestock and poultry | |
| CN103948912B (en) | A kind of livestock and poultry compound medicine containing the radix paeoniae rubrathe and colistin | |
| CN104367601A (en) | Compound medicine containing herba pteridis multifidae and mequindox for livestock and poultry | |
| CN104027370B (en) | A kind of livestock and poultry compound containing Plumbago zeylanica and LIJUNJING | |
| CN104435038A (en) | Balsamiferou blumea herb and mequindox containing compound medicine for livestock and poultry | |
| CN103933085B (en) | The application of Herba Andrographitis and phosphonomycin in the compound medicine for preparing treatment livestock and poultry coli-infection disease | |
| CN104435013A (en) | Pteris multifida and amikacin-containing compound drug for livestock and poultry | |
| CN104840502A (en) | Composition containing epimedium herb and mequindox and used for livestock and poultry, and preparation method thereof | |
| CN104436161A (en) | Pteris multifida and colistin-containing compound drug for livestock and poultry | |
| CN103933087A (en) | Method for treating Escherichia coli infection diseases by using creat and amikacin | |
| CN104162145A (en) | Acalypha australis L. and colistin-containing compound medicine for livestock and poultry | |
| CN105748567A (en) | Compound medicine containing garden balsam stem and mequindox for livestock and poultry | |
| CN103933109A (en) | Method for treating animal escherichia coli infected diseases |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A compound medicine for livestock and poultry containing paeoniae rubra and colistin Effective date of registration: 20210111 Granted publication date: 20160907 Pledgee: Guangxi Beibu Gulf Bank Limited by Share Ltd. small business financial services center Pledgor: GUANGXI NANNING TAOYUAN VETERINARY DRUG FACTORY Registration number: Y2021450000002 |