CN104162145A - Acalypha australis L. and colistin-containing compound medicine for livestock and poultry - Google Patents

Abstract

The invention relates to an Acalypha australis L. and colistin-containing compound medicine for livestock and poultry. The compound medicine comprises, by weight, 3-30 parts of colistin and 5-200 parts of Acalypha australis L.. Researches prove that an antibiotic medicine containing Acalypha australis L. can reverse the drug resistance of bacteria to the antibiotic medicine in order to improve the treatment effect of the antibiotic medicine. The compound medicine can be processed through routine methods to prepare powder, a tablet, an oral liquid or a granule, and the compound medicine is added to a livestock and poultry feed according to a main medicine/feed ratio of 0.1-7g/kg to be used for feeding, so the medicine use cost and medicinal residues are greatly reduced, high economic benefits are created for farmers, and the food safety is ensured.

Description

A kind of poultry compound medicine containing containing pearl grass and colistin

Technical field

The invention belongs to poultry coli-infection treatment technology field, relate in particular to a kind of poultry compound medicine of Guangxi precious jade medicine containing pearl grass and colistin that contain.

Background technology

Escherichia coli are common conditioned pathogens, also be normal a kind of disease of sending out, its multiple location severe infections such as chicken acute sepsis, peritonitis, hepatitis, pneumonia, enteritis of can formerly having carried out the coffin upon burial, also can secondary or mixed sending out in virosis, causes huge economic loss to aquaculture.The beta-lactam lopps antibiotic such as amoxicillin, ampicillin, ceftiofur, ceftriaxone, amoxicillin/clavulanate, ampicillin/sulbactam are the conventional important drugs of this disease of control, along with cultivation scale intensive degree is more and more large, medication is more and more spread unchecked, its Resistant strain is more and more many, also more about the report of Drug Resistance of E. coli.Studies confirm that, one of main mechanism of drug resistance is to produce beta-lactamase, especially extended spectrumβ-lactamase (Extended-Spectrum β-lactamase, ESBLs), this enzyme can be hydrolyzed beta-lactam nucleus more by force and rapidly, makes this class antibiotic lose antibacterial activity.ESBLs is the main enzyme type of beta-lactamase, and it is not only to cephalo three generations and aztreonam drug resistance, and aminoglycoside, quinolones and sulfonamides are to crossing drug resistant, only to carbapenems, a mould alkene class medicaments insensitive.Many documents reported colibacillary multi-drug resistant with produce ESBLs have substantial connection, both at home and abroad to comparatively pay close attention to.The detection, extraction, enzyme that inventor has carried out food animal source pathogenic bacterium ESBLs recently presses down the researchs such as enzyme protection effect to antibiotic percent hydrolysis and sulbactam, Tazobactam Sodium and Chinese medicine; to part ESBLs, colibacillary drug sensitive test shows, its multidrug resistant rate is apparently higher than non-ESBLs bacterial strain.In addition, result also shows to produce ESBLs bacterial strain not only to the serious drug resistance of the third generation cephalosporins such as ceftiofur, and also to multiple antimicrobial drug drug resistance such as fluoroquinolones, aminoglycoside and sulfonamides, fosfomycin etc., is serious multidrug resistant.Inventor, producing the escherichia coli that are separated to tolerate P-levels mycin product fosA3 drug resistant gene in ESBLs bacterial strain, has more strengthened the drug resistance of antibacterial.Colibacillary drug resistance causes raiser's drug cost to increase, and treatment extends the course for the treatment of, and mortality of livestock increases, and brings larger economic loss to production, and increases poultry drug residue risk.Therefore, in actual production, need a kind of to no matter whether, the escherichia coli of drug resistance can have the medicine of better therapeutical effect badly, and can shorten treatment course for the treatment of, reduce mortality rate, reducing economic loss and reduce drug residue, is that raiser creates higher economic benefit, also ensures that the mankind's is healthy and safe.

Summary of the invention

The technical problem to be solved in the present invention is to provide a kind of poultry compound medicine of Guangxi precious jade medicine containing pearl grass and colistin that contain, it can kill antibacterial quickly, overcome antimicrobial drug treatment due to large, the residual height of weak curative effect, consumption and the high defect of cost that drug resistance problem causes, avoided that simple Chinese medicine sterilizing spectrum is narrow, slow problem sterilizes.

For solving above technical problem, the present invention by the following technical solutions: a kind of poultry compound medicine containing containing pearl grass and colistin, is made up of the raw material of following weight portion: colistin 3-30 part, contains the careless 5-200 part of pearl.

Described compound medicine is prepared into powder, tablet, oral liquid or granule.

The preparation method of described compound medicine is: will be containing broken mistake 60 mesh sieve of pearl grass meal, then mix with pulverous antimicrobial drug, be prepared into according to a conventional method powder, tablet, oral liquid or granule.

Containing pearl grass (Acalypha australis L.), have another name called Acalypha australis, Herba Acalyphae, people Herba Amaranthi tricoloris, belong to Euphorbiaceae Acalypha, draft is to suffruticose draft, and stem is very thin, thick below 3 millimeters, female flower bract is lobate, is lobate greatly, and length can reach 1 centimetre, do not divide, there is sawtooth at edge, base portion heart, female flower is kidney shape when open, when closed, is clam, wherein keeps fruit, therefore have the reputation of " Acalypha australis ", also therefore and easily identification.Originate in various places, Guangxi, be born on the ground waste or more moistening place, limit, village.Herb decocts that water is for oral administration can treat bacillary dysentery, diarrhoea etc.

The drug resistance problem existing for current poultry coli-infection, inventor utilizes Guangxi precious jade medicine containing pearl grass and antimicrobial drug composition compound medicine.Studies have shown that, in antimicrobial drug, add containing after pearl grass, can reverse the drug resistance of bacteria medicine, thereby improve the therapeutic effect of antimicrobial drug.According to a conventional method this compound medicine is made to powder, tablet, oral liquid or granule, add in animal and fowl fodder and feed according to the addition of 0.1-7g principal agent/kg feedstuff, can significantly reduce drug cost and drug residue, for raiser creates higher economic benefit, also guarantee food safety simultaneously.

Detailed description of the invention

One, the drug resistance inversion effect research to antimicrobial drug containing pearl grass

Antimicrobial drug itself has the colibacillary effect for the treatment of, but because the escherichia coli that produce ESBLs drug resistant gene have stronger drug resistance, causes antimicrobial drug not show good therapeutic effect in normal using dosage situation.

In order to verify containing the drug resistance inversion effect of pearl grass to antimicrobial drug, on 96 orifice plates, adopt microtest tube doubling dilution, first measured antimicrobial drug to produce ESBLs and fosA3 drug resistant gene e. coli strains minimal inhibitory concentration and containing pearl grass to producing the subinhibitory concentration of ESBLs and fosA3 drug resistant gene e. coli strains, then in culture medium, add reusing microtest tube doubling dilution containing pearl grass and cultivating the minimal inhibitory concentration of measuring antimicrobial drug of subinhibitory concentration; Utilize continuous passage method be added with same subinhibitory concentration containing the culture medium of pearl grass and same concentration antimicrobial drug in to producing the cultivation of going down to posterity of ESBLs and fosA3 drug resistant gene e. coli strains, until the antimicrobial drug minimal inhibitory concentration observing diminishes more than 4 times compared with before going down to posterity, can be judged as containing pearl grass antibacterial medical instrument is had to drug resistance inversion effect.Result of the test statistics is as shown in table 1:

Table 1 subinhibitory concentration (0.5g/mL) goes down to posterity and causes the variation (μ g/mL) of producing ESBLs and fosA3 escherichia coli Western medicine MIC containing pearl grass.

As seen from the above table, antimicrobial drug all reduces more than 4 times the colibacillary minimal inhibitory concentration of drug resistance, can judge containing pearl grass and have drug resistance inversion effect to producing ESBLs and fosA3 drug resistant gene escherichia coli.

Two, the application example of compound medicine of the present invention

Fill a prescription and following methods Preparation Example 1-embodiment 6 according to table 2: will be containing after broken mistake 60 mesh sieves of pearl grass meal, as basic material, again with pulverous antimicrobial drug by weight ratio put into blender and mix, according to a conventional method, compound medicine is prepared into formulation products.

The formula of table 2 embodiment 1-6 and preparation dosage form

Numbering	Antimicrobial drug	Containing pearl grass	Dosage form
				Embodiment 1	Colistin 10g	170g	Powder
Embodiment 2	Colistin 3g	60g	Tablet
				Embodiment 3	Colistin 18g	15g	Oral liquid
Embodiment 4	Colistin 30g	120g	Granule
				Embodiment 5	Colistin 3g	5g	Powder
Embodiment 6	Colistin 30g	200g	Tablet

Inventor, by the compound medicine product of above-described embodiment 1-6 gained, on Guangxi Fangcheng Port pig farm, carries out efficacy experiment to the piglet diarrhea of 35 ages in days.

Clinical symptoms: this swinery after wean is turn-taked, starts to occur diarrhoea, draws watery stools, feces contains indigested feedstuff, pig material feeding but be exactly not grow, and indivedual serious diarrhea of pigs dehydrations are dead, do not occur symptoms of emesis, raiser uses multi-medicament, and ideal effect does not all appear in treatment.

Cut open inspection pathological changes: after cuing open inspection, Intestinum Sus domestica wall is poor, especially small intestinal rear end and colon position, intestinal mucosa comes off, intestinal wall hyperemia, hemorrhage.

Laboratory diagnosis: get pathologic liver and dye sheet, Gram’s staining, micro-Microscopic observation, the gram negative bacilli of red color visible; Tentatively be judged as bacillus coli.Cultivate by escherichia coli, biochemical test is differentiated, judges that Infection in Piglets is bacillus coli, and pcr amplification proves to produce the ESBLs drug resistant gene and the fosA3 drug resistant gene that have CTX-M type.

Test therapeutic regimen: morbidity swinery is divided 8 groups of administrations, use embodiment 1 according to the addition administration of 1.8g/kg feedstuff for first group, use embodiment 2 according to the addition administration of 2g/kg feedstuff for second group, use embodiment 3 according to the addition administration of 0.18g/kg feedstuff for the 3rd group, use embodiment 4 according to the addition administration of 0.4g/kg feedstuff for the 4th group; Use embodiment 5 according to the addition administration of 0.25g/kg feedstuff for the 5th group, use embodiment 6 according to the addition administration of 0.75g/kg feedstuff for the 6th group; Use colistin powder according to the addition administration of 0.1g/kg feedstuff for the 7th group, the 8th group with containing the thick powder of pearl grass according to the addition administration of 4g/kg feedstuff; Above eight groups of therapeutic regimens all continue to use 5 days.

Result of use feedback: first group to the 6th group medication is after 2 days, and swinery is no longer drawn watery stools, feces is shaped, and no longer contains feedstuff in feces, continues to use 3 days swinery recovery from illness; The 7th group of medication is after 2 days, and swinery is still unhealthy, continues to use 3 days, substantially get well, but still draw pasty state feces individually, but drug withdrawal is after 3 days, and recurrence appears in swinery; The 8th group of medication is after 2 days, and swinery, without remarkable improvement, continues to use 3 days, and swinery continues to have loose bowels, and all the other are also more serious individually.Visible, contain containing the compound medicine of pearl grass swinery curative effect is better than to folk prescription.

Claims (2)

1. contain the poultry compound medicine containing pearl grass and colistin, it is characterized in that, formed by the raw material of following weight portion: colistin 3-30 part, containing pearl grass 5-200 part.

2. the poultry compound medicine containing containing pearl grass and colistin according to claim 1, is characterized in that, described compound medicine is prepared into powder, tablet, oral liquid or granule.