CN103989163A - Blood stasis physique health food and making method thereof - Google Patents
Blood stasis physique health food and making method thereof Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Health & Medical Sciences (AREA)
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- Engineering & Computer Science (AREA)
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- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a blood stasis physique health food and a making method thereof. The health food uses medicinal foods comprising peach seed, hawthorn fruit, chicken's gizzard membrane and black garlic as effective medicine components, and auxiliary additives allowed to be used in medicines and foods, and the medicines foods concretely comprise, by weight, 40-15 parts of peach seed, 60-175 parts of hawthorn fruit, 40-160 parts of chicken's gizzard membrane and 30-150 parts of black garlic. The health food aims at blood stasis physique people, and has the efficacies of blood circulation invigorating, blood stasis dissolving, digestion promoting and stagnation removing.
Description
Technical field
The present invention relates to a kind of integration of drinking and medicinal herbs health food and preparation method thereof, be specifically related to for blood stasis physique health food and preparation method thereof.
Background technology
Physique is to form by obtaining congenital heredity and the day after tomorrow, and individuality intrinsic, metastable characteristic aspect morphosis and functional activity, has correlation with psychological personality.Individual physique difference, shows as some otherness in reaction and the adaptability stimulating to external world under physiological status, and the tendentiousness of the neurological susceptibility to some virulence factor and disease progression in pathogenic process.
Tcm constitution Theoretic Source About is in "Nei Jing", the somatotype of the constitutive character to individuality and different groups in "Nei Jing", the formation of physique, physique and all have detailed elaboration with the problem such as relation, the control of disease of disease development.The body and the spirit of tcm constitution theory forms opinion and thinks, physique is the synthesis of specific body quality and certain psychological quality, has embodied " unity of body and soul " thought of the traditional Chinese medical science.
Traditional Chinese Medicine Constitution Classification is taking material base-yin, yang of human life activity, gas, blood, body fluid prosperity and decline state as according to establishing, just because of the prosperity and decline state difference of the interior yin, yang of body, gas, blood, body fluid, thereby show as the physique otherness between individuality.
The understanding of the traditional Chinese medical science to blood stasis physique, starts from "Nei Jing".Blood stasis physique can be coagulated because trembling with fear, the deficiency of vital energy etc. causes.Cold condense into piece, cold is the major reason that causes blood stasis.Cold being a kind of YIN pathogen and main spasm, its property stagnates, no matter exogeneous cold or interior cold all can cause QI-blood circulation not smooth, forms blood stasis.Show as dark complexion, lip look dark, and eye socket is black dull, and scaly dry skin, a symptom of blood stasis is easily hemorrhage, and tongue is purple dark or have petechia, thready and hesitant pulse or a knot generation.If disease above-mentioned feature increases the weight of, can there be head, chest, the side of body, underbelly or four limbs etc. to locate shouting pain; Cyanotic lips or have hemorrhagic tendency, spits blood, just black etc.; Or in abdomen, have abdominal mass to amass piece, dysmenorrhes, through closing, uterine bleeding etc.The main subhealth symptom of blood stasis physique is summed up the Three Character Primer: blood stasis matter, blood thickness, easily forgets things, often long spot.
At present, for improving blood stasis physique symptom, have promoting blood circulation and removing blood stasisly, what disappear the stagnant effect of long-pending row is all some Chinese medicines, and mouthfeel is poor, takes inconvenience.
Summary of the invention
For the problems referred to above of prior art, the invention provides a kind ofly for blood stasis physique, have promoting blood circulation and removing blood stasisly, health food of the stagnant effect of long-pending row and preparation method thereof disappears.
For achieving the above object, the present invention includes following technical scheme:
A kind of blood stasis physique health food, it is to allow the auxiliary adding ingredient composition that uses taking the extract of medicine and food raw materials peach kernel, hawthorn, the membrane of a chicken's gizzard and black garlic as active drug component and in medicine and food, consists of in the crude drug raw material of each effective medicine-food two-purpose component of weight portion: 30~150 parts of 40~125 parts, peach kernel, 60~175 parts of hawthorn, 40~160 parts of thes membrane of a chicken's gizzard and black garlics.
Health food as above, preferably, the crude drug raw material of described each effective medicine-food two-purpose component in weight portion consists of: 50~100 parts of 50~100 parts, peach kernel, 75~125 parts of hawthorn, 80~128 parts of thes membrane of a chicken's gizzard and black garlics.
Health food as above, preferably, the crude drug raw material of described each effective medicine-food two-purpose component in weight portion consists of: 75 parts of 75 parts, peach kernel, 112.5 parts of hawthorn, 90 parts of thes membrane of a chicken's gizzard and black garlics.
Health food as above, preferably, the weight portion composition and ratio of described active drug component is: 3~15 parts of 8~25 parts of Semen Persicae extracts, 12~35 parts of haw thorn extracts, 2.5~10 parts of Endothelium Corneum Gigeriae Galli extracts and black garlic extracts.
Health food as above, preferably, the weight portion composition and ratio of described active drug component is: 5~10 parts of 10~20 parts of Semen Persicae extracts, 15~25 parts of haw thorn extracts, 5~8 parts of Endothelium Corneum Gigeriae Galli extracts and black garlic extracts.
Health food as above, preferably, the weight portion composition and ratio of described active drug component is: 7.5 parts of 15 parts of Semen Persicae extracts, 22.5 parts of haw thorn extracts, 5.63 parts of Endothelium Corneum Gigeriae Galli extracts and black garlic extracts.
Health food as above, preferably, the extracting method of described each extract is as follows:
Peach kernel, hawthorn, the membrane of a chicken's gizzard and black garlic all use decocting cooking method to obtain extract.
Health food as above, preferably, also contains auxiliary material in this health food, be selected from one or more in stir-bake to yellowish ground rice, purple sweet potato powder, mung bean flour, black sesame powder, konjaku flour and mogroside.
Health food as above, preferably, the health food of every 100 weight portions is containing 0.4~1.6 part of 10~40 parts, stir-bake to yellowish ground rice, 10~35 parts of purple sweet potato powders, 6~20 parts of mung bean flours, 4~15 parts of black sesame powders, 2.5~10 parts of konjaku flours and/or mogroside.
Health food as above, preferably, the preparation method of described Semen Persicae extract is as follows:
Peach kernel, adds 4-10 times of water gaging and boils extraction 0.5-2 hour, extracts 1-3 time, and extracting liquid filtering, merges filtered fluid for subsequent use; Filtered fluid is concentrated, is concentrated into proportion 1.06-1.14; Concentrate is centrifugal with centrifuge, and extracting centrifugal liquid is for subsequent use; After centrifugate adds the 5-35% maltodextrin of output to mix, to spray dryer, be dried, spray dryer EAT is 180-205 DEG C, and leaving air temp is 90-105 DEG C; Get the dry gained dried cream powder of spraying broken, cross 80 orders, obtain Semen Persicae extract.
Health food as above, preferably, the preparation method of described haw thorn extract is as follows:
Hawthorn, adds 4-10 times of water gaging and boils extraction 0.5-2 hour, extracts 1-3 time, and extracting liquid filtering, merges filtered fluid for subsequent use; Filtered fluid is concentrated, is concentrated into proportion 1.06-1.14; Concentrate is centrifugal with centrifuge, and extracting centrifugal liquid is for subsequent use; After centrifugate adds the 5-15% maltodextrin of output to mix, to spray dryer, be dried, spray dryer EAT is 180-205 DEG C, and leaving air temp is 90-105 DEG C; Get the dry gained dried cream powder of spraying broken, cross 80 orders, obtain haw thorn extract.
Health food as above, preferably, the preparation method of described Endothelium Corneum Gigeriae Galli extract is as follows:
The membrane of a chicken's gizzard medicine materical crude slice, coarse crushing, to 0.5cm-1.5cm, adds 5-12 times of water gaging and boils extraction 1-3 hour, extracts 1-3 time, and extracting liquid filtering, merges filtered fluid for subsequent use; Filtered fluid is concentrated, is concentrated into proportion 1.06-1.14; Concentrate is centrifugal with centrifuge, and extracting centrifugal liquid is for subsequent use; After centrifugate adds the 15-25% maltodextrin of output to mix, to spray dryer, be dried, spray dryer EAT is 180-205 DEG C, and leaving air temp is 90-105 DEG C; Get the dry gained dried cream powder of spraying broken, cross 80 mesh sieves, obtain Endothelium Corneum Gigeriae Galli extract.
Health food as above, preferably, the preparation method of described black garlic extract is as follows:
Black garlic, adds 4-10 times of water gaging and boils extraction 0.5-2 hour, extracts 1-3, and extracting liquid filtering, merges filtered fluid for subsequent use; Filtered fluid is concentrated, is concentrated into proportion 1.06-1.14; Concentrate is centrifugal with centrifuge, and extracting centrifugal liquid is for subsequent use; After centrifugate adds the 10-20% maltodextrin of output to mix, to spray dryer, be dried, spray dryer EAT is 180-205 DEG C, and leaving air temp is 90-105 DEG C; Get the dry gained dried cream powder of spraying broken, cross 80 mesh sieves, obtain black garlic extract.
Hawthorn in the present invention hawthorn of preferably making a living.
Health food of the present invention, for blood stasis physique crowd, in the time producing, can increase or reduce according to corresponding ratio, as large-scale production can with kilogram or taking ton as unit, small-scale production also can be in grams, weight can increase or reduce, but raw material weight proportioning constant rate between each composition.
On the other hand, the invention provides the preparation method of above-mentioned health food, the method comprises the following steps: described mogroside is added water and makes slurry; Stir-bake to yellowish ground rice, peach kernel powder, hawthorn powder, the membrane of a chicken's gizzard powder, black garlic powder, mung bean flour, black sesame powder, konjaku flour, purple sweet potato powder are dropped in granulator successively.First dry and mix for 10 minutes, treat that EAT rises to 73 DEG C, the Momordica grosvenori slurry of making is sprayed in fluid bed granulator, rising granulation, sieves, filling, to obtain final product.
On the one hand, the invention provides composition as above in the application of preparing in blood stasis physique health food and medicine again.
Application as above, preferably, the dosage of described health food is 5-15g/ days.
The present invention is directed to blood stasis physique crowd, have promoting blood circulation and removing blood stasis, the health food of the stagnant effect of long-pending row that disappears, its beneficial effect is, formula Design adopts the frame structure of traditional Chinese medicine " monarch, minister, help, make ".In side, monarch drug in a prescription peach kernel, promoting blood circulation and removing blood stasis.Shennong's Herbal " main hemostasis, blood closes abdominal mass, perverse trend is killed small worm " is double moisturizes gut purge.The loose stasis of blood row of ministerial drug hawthorn is stagnant, " Records of Tradition Chinese and Western Medicine in Combination ": " hawthorn, Removing Blood Stasis and do not hinder new blood are opened strongly fragrant gas and do not hindered healthy tendency ".The adjutant the membrane of a chicken's gizzard, stagnation resolvation disappears long-pending, " Records of Tradition Chinese and Western Medicine in Combination ": " stagnant to change the stasis of blood of its channels and collaterals ... the kind Removing Blood Stasis of the lid the membrane of a chicken's gizzard ".Make medicine garlic, stagnation resolvation is sensible, guides all medicines, and through sick institute, takes preventive measures.Compendium of Material Medica: " its gas is smoked strong, can lead to the five internal organs, reaches all keys, and cold removing is wet, the evil that exorcises evil spirits, subduing inflammation, changes disease long-pending carnivorous ".All medicines share, and play altogether promoting blood circulation and removing blood stasisly, and the stagnant merit of long-pending row disappears.
Health food of the present invention carries out compatibility with medicine-food two-purpose food materials, nurses one's health the biased of human body constitution, and curative effect is reliable, has no side effect, and taste is acceptant,, containing any anticorrisive agent, synthetic dyestuff, compound essence, does not have good stability, and is easy to carry.
Detailed description of the invention
Primary raw material of the present invention is peach kernel, hawthorn, the membrane of a chicken's gizzard and black garlic.
Peach kernel: sweet, bitter, flat, the thoughts of returning home, liver, large intestine channel.Promoting blood circulation and removing blood stasis, relax bowel, relieving cough and asthma.
Hawthorn: sour, sweet, tepor, return spleen, stomach, Liver Channel.Food digesting stomach fortifying, the promoting the circulation of qi stasis of blood of faling apart, changes turbid lipopenicillinase.
The membrane of a chicken's gizzard: sweet, flat.Return spleen, stomach, small intestine, urinary bladder channel.Stomach strengthening and digestion promoting, treating stranguria fossil.
Black garlic: pungent, warm.Return spleen, stomach, lung channel.Removing toxicity for detumescence.Black garlic has another name called fermenting black garlic, is with fresh raw garlic, the food that fermentation is made, and the micronutrient levels in black garlic is higher, and taste is sour-sweet, without garlicky, has kept again effect of garlic simultaneously, has effect anti-oxidant, antiacidization, is particularly suitable for three high crowds.
Stir-bake to yellowish ground rice, as the excipient of product, improves products taste.
Purple sweet potato powder, as the excipient of product, improves products taste.
Mung bean flour, as the excipient of product, improves products taste.
Black sesame powder, as the excipient of product, improves products taste.
Konjaku flour, utilizes its thickening property, makes product reconstitute the uniform paste of rear formation, is convenient to take.
Mogroside: natural sweetener, improve the taste of product, meet the requirement of consumer to mouthfeel.
Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.In following examples, source or the preparation method of the raw material that uses are as follows:
1, the preparation method of Semen Persicae extract
Peach kernel, adds 8 times of water gagings and boils extraction 1.5 hours, extracting liquid filtering, and the dregs of a decoction add 6 times of water gagings and boil extraction 1 hour, and extracting liquid filtering merges filtered fluid for subsequent use.Filtered fluid is concentrated, is concentrated into proportion 1.06-1.14; Concentrate is centrifugal with centrifuge, and extracting centrifugal liquid is for subsequent use; After centrifugate adds 10% maltodextrin of output to mix, to spray dryer, be dried, spray dryer EAT is 180-205 DEG C, and leaving air temp is 90-105 DEG C; Get the dry gained dried cream powder of spraying broken, cross 80 orders, obtain Semen Persicae extract.
Through above-mentioned steps, every 100 weight portion crude drug raw materials obtain extract approximately 20 weight portions.
2, the preparation method of haw thorn extract
Hawthorn, adds 8 times of water gagings and boils extraction 1.5 hours, extracting liquid filtering, and the dregs of a decoction add 6 times of water gagings and boil extraction 1 hour, and extracting liquid filtering merges filtered fluid for subsequent use.Filtered fluid is concentrated, is concentrated into proportion 1.06-1.14; Concentrate is centrifugal with centrifuge, and extracting centrifugal liquid is for subsequent use; After centrifugate adds 10% maltodextrin of output to mix, to spray dryer, be dried, spray dryer EAT is 180-205 DEG C, and leaving air temp is 90-105 DEG C; Get the dry gained dried cream powder of spraying broken, cross 80 orders, obtain haw thorn extract.
Through above-mentioned steps, every 100 weight portion crude drug raw materials obtain extract approximately 20 weight portions.
3, the preparation method of Endothelium Corneum Gigeriae Galli extract
The membrane of a chicken's gizzard medicinal material, coarse crushing, to 1cm left and right size, adds 8 times of water gagings and boils extraction 2.0 hours, extracting liquid filtering, the dregs of a decoction add 8 times of water gagings and boil extraction 1.5 hours, and extracting liquid filtering merges filtered fluid for subsequent use.Filtered fluid is concentrated, is concentrated into proportion 1.06-1.14; Concentrate is centrifugal with centrifuge, and extracting centrifugal liquid is for subsequent use; After centrifugate adds 20% maltodextrin of output to mix, to spray dryer, be dried, spray dryer EAT is 180-205 DEG C, and leaving air temp is 90-105 DEG C; Get the dry gained dried cream powder of spraying broken, cross 80 mesh sieves, obtain Endothelium Corneum Gigeriae Galli extract.
Through above-mentioned steps, every 100 weight portion crude drug raw materials obtain extract approximately 6.25 weight portions.
4, the preparation method of black garlic extract
Black garlic, adds 8 times of water gagings and boils extraction 1.5 hours, extracting liquid filtering, and the dregs of a decoction add 6 times of water gagings and boil extraction 1 hour, and extracting liquid filtering merges filtered fluid for subsequent use.Concentrate is centrifugal with centrifuge, and extracting centrifugal liquid is for subsequent use; After centrifugate adds 16% maltodextrin of output to mix, to spray dryer, be dried, spray dryer EAT is 180-205 DEG C, and leaving air temp is 90-105 DEG C; Get the dry gained dried cream powder of spraying broken, cross 80 mesh sieves, obtain black garlic extract.Through above-mentioned steps, every 100 weight portion crude drug raw materials obtain extract approximately 10 weight portions.
5, stir-bake to yellowish ground rice is commercial, purchased from Hunan Kang Rui fine jade bio tech ltd, lot number CHMF130727.
6, purple sweet potato powder is commercial, purchased from Beijing Sheng Lun Food Co., Ltd, lot number SCA-41-13-2.
7, mung bean flour is commercial, purchased from Beijing Sheng Lun Food Co., Ltd, lot number SCA-58H-13-1.
8, black sesame powder is commercial, purchased from Beijing Sheng Lun Food Co., Ltd, lot number SCA-60-13-1.
9, konjaku flour is commercial, purchased from Shantou Jiecheng Biotechnology Co., Ltd., and lot number 20131015.
10, mogroside, buys from Ningbo Liwah Plant Extract Technology Co., Ltd. lot number 20131001.
Embodiment 1: the health food () of blood stasis physique
1, crude drug raw material composition: peach kernel 75 weight portions, the membrane of a chicken's gizzard 90 weight portions, hawthorn 112.5 weight portions, black garlic 75 weight portions;
Make the composition after extract: Semen Persicae extract 15 weight portions, Endothelium Corneum Gigeriae Galli extract 5.63 weight portions, haw thorn extract 22.5 weight portions, black garlic extract 7.5 weight portions;
Auxiliary material: stir-bake to yellowish ground rice 30.66 weight portions, purple sweet potato powder 30 weight portions, mung bean flour 18 weight portions, black sesame powder 12 weight portions, konjaku flour 7.5 weight portions and mogroside 1.2 weight portions.
2, preparation process:
1) weigh: total inventory 150kg, weigh according to formula ratio, for subsequent use.
2) slurrying: by weighted mogroside, be dissolved in formula total amount 20% water in (30kg), for subsequent use.
3) boiling granulating: stir-bake to yellowish ground rice, peach kernel powder, hawthorn powder, the membrane of a chicken's gizzard powder, black garlic powder, mung bean flour, black sesame powder, konjaku flour, purple sweet potato powder are dropped in granulator successively.First dry and mix for 10 minutes, treat that EAT rises to 73 DEG C, the Momordica grosvenori slurry that step 2 is made sprays in fluid bed granulator, rising granulation, material moisture≤4.0% discharging.
4) sieve: 18 order vibratory sieves.
5) filling (inner packing): carry out fillingly with measuring cup type 10 row machines, 5g/ bag, obtains the health food (product 1) of blood stasis physique.
Embodiment 2: the health food (two) of blood stasis physique
1, crude drug raw material composition: peach kernel 125 weight portions, the membrane of a chicken's gizzard 160 weight portions, hawthorn 150 weight portions, black garlic 100 weight portions;
Make the composition after extract: Semen Persicae extract 25 weight portions, Endothelium Corneum Gigeriae Galli extract 10 weight portions, haw thorn extract 30 weight portions, black garlic extract 10 weight portions;
Auxiliary material: stir-bake to yellowish ground rice 30 weight portions, purple sweet potato powder 30 weight portions, mung bean flour 20 weight portions, black sesame powder 15 weight portions, konjaku flour 10 weight portions and mogroside 1.5 weight portions.
2, the health food (product 2) of blood stasis physique is prepared in the employing operation identical with embodiment 1 preparation process.
Embodiment 3: the health food (three) of blood stasis physique
1, crude drug raw material composition: peach kernel 45 weight portions, the membrane of a chicken's gizzard 48 weight portions, hawthorn 75 weight portions, black garlic 40 weight portions;
Make the composition after extract: Semen Persicae extract 9 weight portions, Endothelium Corneum Gigeriae Galli extract 3 weight portions, haw thorn extract 15 weight portions, black garlic extract 4 weight portions;
Auxiliary material: stir-bake to yellowish ground rice 15 weight portions, purple sweet potato powder 12 weight portions, mung bean flour 8 weight portions, black sesame powder 5 weight portions, konjaku flour 3 weight portions and mogroside 0.5 weight portion.
2, the health food (product 3) of blood stasis physique is prepared in the employing operation identical with embodiment 1 preparation process.Embodiment 4: the health food (four) of blood stasis physique
1, crude drug raw material composition: peach kernel 100 weight portions, the membrane of a chicken's gizzard 160 weight portions, hawthorn 175 weight portions, black garlic 150 weight portions;
Make the composition after extract: Semen Persicae extract 25 weight portions, Endothelium Corneum Gigeriae Galli extract 10 weight portions, haw thorn extract 35 weight portions, black garlic extract 15 weight portions;
Auxiliary material: stir-bake to yellowish ground rice 25 weight portions, purple sweet potato powder 20 weight portions, mung bean flour 18 weight portions, black sesame powder 8 weight portions, konjaku flour 8 weight portions and mogroside 0.7 weight portion.
2, the health food (product 4) of blood stasis physique is prepared in the employing operation identical with embodiment 1 preparation process.Embodiment 5: the health food (five) of blood stasis physique
1, crude drug raw material composition: peach kernel 60 weight portions, the membrane of a chicken's gizzard 80 weight portions, hawthorn 100 weight portions, black garlic 50 weight portions;
Make the composition after extract: Semen Persicae extract 20 weight portions, Endothelium Corneum Gigeriae Galli extract 5 weight portions, haw thorn extract 20 weight portions, black garlic extract 5 amounts part;
Auxiliary material: stir-bake to yellowish ground rice 35 weight portions, purple sweet potato powder 16 weight portions, mung bean flour 12 weight portions, black sesame powder 8 weight portions, konjaku flour 3 weight portions and mogroside 1.3 weight portions.
2, the health food (product 5) of blood stasis physique is prepared in the employing operation identical with embodiment 1 preparation process.
Embodiment 6: the health food (six) of blood stasis physique
1, crude drug raw material composition: peach kernel 90 weight portions, the membrane of a chicken's gizzard 112 weight portions, hawthorn 90 weight portions, black garlic 90 weight portions;
Make the composition after extract: Semen Persicae extract 18 weight portions, Endothelium Corneum Gigeriae Galli extract 7 weight portions, haw thorn extract 18 weight portions, black garlic extract 9 weight portions;
Auxiliary material: stir-bake to yellowish ground rice 30 weight portions, purple sweet potato powder 30 weight portions, mung bean flour 10 weight portions, black sesame powder 12 weight portions, konjaku flour 6 weight portions and mogroside 0.6 weight portion.
2, the health food (product 6) of blood stasis physique is prepared in the employing operation identical with embodiment 1 preparation process.
The auxiliary stasis syndrome mouse function test of improving of experimental example 1 blood stasis product
One, experimental principle
Blood stasis is one of common clinically disease, and can coagulate because trembling with fear, the deficiency of vital energy etc. causes.Cold condense into piece, cold is the major reason that causes blood stasis.Cold being a kind of YIN pathogen and main spasm, its property stagnates, no matter exogeneous cold or interior cold all can cause QI-blood circulation not smooth, forms blood stasis.Frozen water is not only cold stimulation to mouse, is subject to terrified stimulation in water environment, also will consume more energy.So, in test, select adrenaline to shrink peripheral vascular, increase myocardial contractive power, myocardial metabolism oxygen consumption is increased, heart function declines gradually, thereby makes blood circulation and microcirculation disorder, gives cold stimulation simultaneously, adopt the method for the acute modeling of cold blood stasis of complex factors, study blood stasis product and whether there is improvement effect.This experiment is the impact at aspects such as Hemorheology, microcirculation, clotting times on cold blood stasis card animal model by blood stasis product, observes the function of promoting blood circulation to disperse blood clots of blood stasis product.
Two. test material:
Test sample: blood stasis product (product 1-3 prepared by embodiment 1-3).
Reference substance: (Jilin Aodong Yanbian Medicine Industry Co., Ltd produces Xuefu Zhuyu oral liquid, batch number: 1212009), (peach garden, Nanjing Applied Biotechnology Co., Ltd produces peach kernel tea, batch number: 20120831).
Chemicals: adrenalin hydrochloride parenteral solution, batch number: 121102, Shanghai Hefeng Pharmaceutical Co., Ltd..
Instrument: Sweden Perimed Laser doppler seanning, PeriFlux5001; U.S. Brookfield blood rheological instrument.
Experimental animal: ICR mouse is tieed up Co., Ltd of tonneau China purchased from Beijing, the quality certification number: SCXK (capital) 2007-0001 is male, 22.7 ± 0.11g.Raise in the SPF of China Medical Sciences Academy Medical Plants Institute level Animal House.
Three. test method:
ICR mouse, be divided into 8 groups, normal group, model contrast, 1 four dosage groups of blood stasis product (0.417g/kg, 0.834g/kg, 1.668g/kg, 3.336g/kg) (being equivalent to respectively 2.5,5.0,10 and 20 times of amounts of people's consumption 10.0g/60kg), blood stasis product 2,3 dosage groups (3.336g/kg), positive drug contrast Xuefu Zhuyu oral liquid (5.0ml/kg) and peach kernel tea (0.53g/kg), given the test agent gives 15 days, gavage volume is 10mL/kg, and blank group and model group gavage give equal-volume physiological saline.After 14d gavage, model group and each administration group be hypodermic injection adrenalin hydrochloride respectively, and volume is 1mL/kg, water is can't help in fasting, after 2h, respectively organize mouse ice-water bath swimming 5min, again inject the adrenalin hydrochloride of same dose after 2h, blank group is injected equal-volume physiological saline.0.5h after last gastric infusion, animal subject, with 10% chloral hydrate anesthesia, is fixed on experimental bench, the situation of change of auricular microcirculation when 40min after medicine is observed and measured to Doppler's microcirculation tester, 1h after administration, respectively organizes eye socket veniplex and gets blood, measures the clotting time.
Four. statistical method
Measurement data is mean value ± standard error (mean ± standard error) expression for data, and each group difference relatively adopts t inspection, is completed by EXCEL statistical software.P < 0.05 is that there were significant differences, and p < 0.01 is for there being utmost point significant difference.
Five. result of the test:
1, the impact of blood stasis product on Mouse Weight:
Blood stasis product gavage mouse two weeks, each dosage group is showed no obvious impact to the growth of Mouse Weight.Illustrate that this product can not put on weight.
The impact (n=10) of table 1 blood stasis product on Mouse Weight
Group | Zero circle | One week | Two weeks |
Normal group | 22.70±0.59 | 26.32±0.60 | 29.56±0.81 |
Model control group | 22.75±0.32 | 27.16±0.50 | 29.20±0.38 |
Product 1 (0.417g/kg) | 22.85±0.21 | 26.47±0.42 | 28.43±0.51 |
Product 1 (0.834g/kg) | 22.79±0.35 | 26.74±0.48 | 28.74±0.62 |
Product 1 (1.668g/kg) | 22.61±0.19 | 27.31±0.37 | 29.28±0.43 |
Product 1 (3.336g/kg) | 22.70±0.27 | 26.78±0.40 | 28.74±0.52 |
Product 2 (3.336g/kg) | 22.55±0.20 | 26.42±0.35 | 28.16±0.45 |
Product 3 (3.336g/kg) | 22.60±0.23 | 26.24±0.37 | 28.35±0.29 |
Xuefu Zhuyu oral liquid | 22.71±0.28 | 26.94±0.66 | 27.98±0.56 |
Peach kernel tea | 22.53±0.18 | 26.21±0.36 | 28.20±0.47 |
2, blood stasis product improves microcirculatory effect
With the comparison of normal control mouse, model group Mice Auricle microcirculation flow reduces 78.27%.The mouse of the each dosage group of blood stasis product 1 of feeding, with the comparison of model group mouse, auricular microcirculation flow obviously increases by 213.2%, 248.1%, 248.0% and 252.5% (p < 0.01), the mouse that product 2, product are 3 groups, with the comparison of model group mouse, obvious 249.9%, 249.9% (the p < 0.01) of auricular microcirculation flow, shows the microcirculatory effect of stasis syndrome mouse that has clear improvement of blood stasis product tool.Refer to table 2.
Table 2 blood stasis product is on the microcirculatory impact of Mice Auricle (n=10)
Group | Auricular microcirculation (bpu) |
Normal group | 716.67±47.67 |
Model control group | 155.70±29.37 ## |
Product 1 (0.417g/kg) | 487.60±88.77 ** |
Product 1 (0.834g/kg) | 542.00±56.37 ** |
Product 1 (1.668g/kg) | 541.90±80.92 ** |
Product 1 (3.336g/kg) | 548.90±47.13 ** |
Product 2 (3.336g/kg) | 544.75±75.36 ** |
Product 3 (3.336g/kg) | 545.25±79.90 ** |
Xuefu Zhuyu oral liquid | 438.40±68.79 ** |
Peach kernel tea | 542.11±98.64 ** |
With normal group comparison,
##p < 0.01, has utmost point significant difference; With model group comparison,
*p < 0.01, has utmost point significant difference
3, the effect of blood stasis product to the clotting time
With the comparison of normal control mouse, model group clotting time of mice has shortened 6.88%.With the comparison of model group mouse, the clotting time of the mouse of the each dosage group of blood stasis product of feeding has no obvious change, shows that blood stasis product has no obvious improvement effect to the clotting time.Refer to table 3.
The impact (n=10) of table 3 blood stasis product on clotting time of mice
Group | Clotting time (s) |
Normal group | 307.67±20.39 |
Model control group | 286.50±12.07 |
Product 1 (0.417g/kg) | 242.30±24.37 |
Product 1 (0.834g/kg) | 289.50±26.62 |
Product 1 (1.668g/kg) | 283.80±32.12 |
Product 1 (3.336g/kg) | 285.20±25.54 |
Product 2 (3.336g/kg) | 284.14±27.43 |
Product 3 (3.336g/kg) | 286.35±25.18 |
Xuefu Zhuyu oral liquid | 301.56±24.98 |
Peach kernel tea | 215.33±22.92 |
4, the effect of blood stasis product to whole blood viscosity
With the comparison of normal control mouse, under model group mouse low shear rate, whole blood viscosity has raise 14.30%.With the comparison of model group mouse, under the each dosage group of blood stasis product 1 mouse low shear rate, whole blood viscosity obviously reduces by 23.47% (p < 0.05), 31.66% (p < 0.01), 33.77% (p < 0.01) and 43.84% (p < 0.01).Under 2,3 groups of mouse low shear rates of blood stasis product, whole blood viscosity obviously reduces.
With the comparison of normal control mouse, in model group mouse, under shear rate, whole blood viscosity has raise 8.70%.With the comparison of model group mouse, in blood stasis product 11.668g/kg and 3.336g/kg dosage group mouse, under shear rate, whole blood viscosity obviously reduces by 24.74% (p < 0.05), 29.54% (p < 0.05).
With the comparison of normal control mouse, under the high shear rate of model group mouse, whole blood viscosity has raise 1.02%.With the comparison of model group mouse, under blood stasis product 11.668g/kg and the high shear rate of 3.336g/kg dosage group mouse, whole blood viscosity obviously reduces by 17.22% (p < 0.05), 30.22% (p < 0.05).Under the high shear rate of blood stasis product 2,3 dosage group mouse, whole blood viscosity obviously reduces.
Above result shows the have clear improvement effect of stasis syndrome mouse whole blood viscosity of blood stasis product tool, refers to table 4.
The impact (n=10) of table 4 blood stasis product on mouse whole blood viscosity
Group | High shear rate | Middle shear rate | Low shear rate |
Normal group | 10.96±0.43 | 13.88±0.78 | 226.40±11.07 |
Model control group | 11.08±0.24 | 15.20±0.59 | 264.17±14.37 |
Product 1 (0.417g/kg) | 9.92±0.63 | 12.79±1.41 | 202.16±17.65** |
Product 1 (0.834g/kg) | 9.43±0.60 | 12.37±1.22 | 180.52±20.56 ** |
Product 1 (1.668g/kg) | 9.16±0.75* | 11.44±1.49* | 174.95±21.10 ** |
Product 1 (3.336g/kg) | 8.07±1.12* | 10.71±1.48* | 148.35±25.71 ** |
Product 2 (3.336g/kg) | 9.03±0.82* | 11.32±1.27* | 173.59±20.16 ** |
Product 3 (3.336g/kg) | 9.12±0.37* | 11.48±1.36* | 168.87±21.19 ** |
Xuefu Zhuyu oral liquid | 10.58±0.12 | 14.68±0.98 | 205.11±12.80 * |
Peach kernel tea | 10.09±0.88 | 13.42±1.79 | 196.08±15.02 ** |
With model group comparison,
*p < 0.05, has significant difference,
*p < 0.01, has utmost point significant difference
Six, conclusion
Above-mentioned test explanation blood stasis product has good effect improving blood viscosity and improve aspect microcirculation.
Claims (10)
1. a blood stasis physique health food, it is characterized in that, allow the auxiliary adding ingredient composition using taking the extract of medicine and food raw materials peach kernel, hawthorn, the membrane of a chicken's gizzard and black garlic as active drug component and in medicine and food, consist of in the crude drug raw material of each effective medicine-food two-purpose component of weight portion: 30~150 parts of 40~125 parts, peach kernel, 60~175 parts of hawthorn, 40~160 parts of thes membrane of a chicken's gizzard and black garlics.
2. health food as claimed in claim 1, is characterized in that, the crude drug raw material of described each effective medicine-food two-purpose component in weight portion consists of: 50~100 parts of 50~100 parts, peach kernel, 75~125 parts of hawthorn, 80~128 parts of thes membrane of a chicken's gizzard and black garlics.
3. health food as claimed in claim 1, is characterized in that, the crude drug raw material of described each effective medicine-food two-purpose component in weight portion consists of: 75 parts of 75 parts, peach kernel, 112.5 parts of hawthorn, 90 parts of thes membrane of a chicken's gizzard and black garlics.
4. health food as claimed in claim 1, is characterized in that, the weight portion composition and ratio of described active drug component is: 3~15 parts of 8~25 parts of Semen Persicae extracts, 12~35 parts of haw thorn extracts, 2.5~10 parts of Endothelium Corneum Gigeriae Galli extracts and black garlic extracts.
5. health food according to claim 4, is characterized in that, the weight portion composition and ratio of described active drug component is: 5~10 parts of 10~20 parts of Semen Persicae extracts, 15~25 parts of haw thorn extracts, 5~8 parts of Endothelium Corneum Gigeriae Galli extracts and black garlic extracts.
6. health food according to claim 5, is characterized in that, the weight portion composition and ratio of described active drug component is: 7.5 parts of 15 parts of Semen Persicae extracts, 22.5 parts of haw thorn extracts, 5.63 parts of Endothelium Corneum Gigeriae Galli extracts and black garlic extracts.
7. according to the health food described in claim 1-6 any one, it is characterized in that, the extracting method of described each extract is as follows:
Peach kernel, hawthorn, the membrane of a chicken's gizzard and black garlic all use decocting cooking method to obtain extract.
8. according to the health food described in claim 1-7 any one, it is characterized in that, in this health food, also contain auxiliary material, be selected from one or more in stir-bake to yellowish ground rice, purple sweet potato powder, mung bean flour, black sesame powder, konjaku flour and mogroside.
9. health food according to claim 8, it is characterized in that, the health food of every 100 weight portions is containing 0.4~1.6 part of 10~40 parts, stir-bake to yellowish ground rice, 10~35 parts of purple sweet potato powders, 6~20 parts of mung bean flours, 4~15 parts of black sesame powders, 2.5~10 parts of konjaku flours and/or mogroside.
10. the health food described in claim 1-9 any one is in the application of preparing in blood stasis physique health food and medicine.
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CN107751991A (en) * | 2016-08-22 | 2018-03-06 | 江苏康缘药业股份有限公司 | A kind of health-care food composition of invigorating kidney, promoting blood circulation and preparation method thereof |
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CN110794074A (en) * | 2019-11-18 | 2020-02-14 | 广西医科大学 | Angelica sinensis Sini decoction cold-resistant blood coagulation stasis syndrome differential metabolite metabolic pathway and research method |
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CN104983843A (en) * | 2015-08-07 | 2015-10-21 | 南京同仁堂黄山精制药业有限公司 | Traditional Chinese medicine composite and preparation thereof |
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CN107751991A (en) * | 2016-08-22 | 2018-03-06 | 江苏康缘药业股份有限公司 | A kind of health-care food composition of invigorating kidney, promoting blood circulation and preparation method thereof |
CN109793132A (en) * | 2019-04-03 | 2019-05-24 | 吉林省玖壹农业科技发展有限公司 | A kind of degrading the channel improves the liquid beverage and preparation method thereof of microcirculation |
CN110794074A (en) * | 2019-11-18 | 2020-02-14 | 广西医科大学 | Angelica sinensis Sini decoction cold-resistant blood coagulation stasis syndrome differential metabolite metabolic pathway and research method |
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