CN103981118B - 一种枯草芽孢杆菌饲料添加剂及其制备方法和应用 - Google Patents
一种枯草芽孢杆菌饲料添加剂及其制备方法和应用 Download PDFInfo
- Publication number
- CN103981118B CN103981118B CN201310719942.0A CN201310719942A CN103981118B CN 103981118 B CN103981118 B CN 103981118B CN 201310719942 A CN201310719942 A CN 201310719942A CN 103981118 B CN103981118 B CN 103981118B
- Authority
- CN
- China
- Prior art keywords
- bacillus subtilis
- wei
- fermentation
- seed
- bacterium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 244000063299 Bacillus subtilis Species 0.000 title claims abstract description 145
- 235000014469 Bacillus subtilis Nutrition 0.000 title claims abstract description 145
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 241000894006 Bacteria Species 0.000 claims abstract description 68
- 238000000855 fermentation Methods 0.000 claims abstract description 38
- 230000004151 fermentation Effects 0.000 claims abstract description 38
- 239000000654 additive Substances 0.000 claims abstract description 29
- 230000000996 additive effect Effects 0.000 claims abstract description 29
- 239000000843 powder Substances 0.000 claims abstract description 27
- 239000007788 liquid Substances 0.000 claims abstract description 26
- 241001465754 Metazoa Species 0.000 claims abstract description 25
- 238000004519 manufacturing process Methods 0.000 claims abstract description 23
- 230000000694 effects Effects 0.000 claims abstract description 20
- 238000001694 spray drying Methods 0.000 claims abstract description 6
- 239000012530 fluid Substances 0.000 claims description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 17
- 239000001963 growth medium Substances 0.000 claims description 14
- 239000002609 medium Substances 0.000 claims description 11
- 239000000203 mixture Substances 0.000 claims description 10
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 9
- 239000001888 Peptone Substances 0.000 claims description 9
- 108010080698 Peptones Proteins 0.000 claims description 9
- 235000019319 peptone Nutrition 0.000 claims description 9
- 238000004321 preservation Methods 0.000 claims description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 7
- 239000006916 nutrient agar Substances 0.000 claims description 7
- 238000011218 seed culture Methods 0.000 claims description 7
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- 239000002518 antifoaming agent Substances 0.000 claims description 6
- 229940041514 candida albicans extract Drugs 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 6
- ISPYRSDWRDQNSW-UHFFFAOYSA-L manganese(II) sulfate monohydrate Chemical compound O.[Mn+2].[O-]S([O-])(=O)=O ISPYRSDWRDQNSW-UHFFFAOYSA-L 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 239000012138 yeast extract Substances 0.000 claims description 6
- 229920002472 Starch Polymers 0.000 claims description 5
- 230000004913 activation Effects 0.000 claims description 5
- 239000008107 starch Substances 0.000 claims description 5
- 235000019698 starch Nutrition 0.000 claims description 5
- 229920001817 Agar Polymers 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- KVCWSJZUKMSPLM-UHFFFAOYSA-N O.O[PH2]=O Chemical compound O.O[PH2]=O KVCWSJZUKMSPLM-UHFFFAOYSA-N 0.000 claims description 4
- 239000008272 agar Substances 0.000 claims description 4
- KCIDZIIHRGYJAE-YGFYJFDDSA-L dipotassium;[(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] phosphate Chemical compound [K+].[K+].OC[C@H]1O[C@H](OP([O-])([O-])=O)[C@H](O)[C@@H](O)[C@H]1O KCIDZIIHRGYJAE-YGFYJFDDSA-L 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 239000001257 hydrogen Substances 0.000 claims description 4
- 229910052739 hydrogen Inorganic materials 0.000 claims description 4
- 125000004435 hydrogen atom Chemical class [H]* 0.000 claims description 4
- 239000010977 jade Substances 0.000 claims description 4
- 230000035764 nutrition Effects 0.000 claims description 4
- 235000016709 nutrition Nutrition 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 238000010009 beating Methods 0.000 claims description 3
- 235000015278 beef Nutrition 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 239000000284 extract Substances 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 238000010899 nucleation Methods 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- 230000006641 stabilisation Effects 0.000 claims description 3
- 238000011105 stabilization Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 2
- 238000009423 ventilation Methods 0.000 claims description 2
- 230000003716 rejuvenation Effects 0.000 claims 1
- 238000005507 spraying Methods 0.000 claims 1
- 244000144977 poultry Species 0.000 abstract description 28
- 244000144972 livestock Species 0.000 abstract description 22
- 230000012010 growth Effects 0.000 abstract description 21
- 230000002496 gastric effect Effects 0.000 abstract description 15
- 230000001580 bacterial effect Effects 0.000 abstract description 14
- 229940099352 cholate Drugs 0.000 abstract description 11
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 abstract description 11
- 238000000034 method Methods 0.000 abstract description 9
- 238000009395 breeding Methods 0.000 abstract description 8
- 230000001488 breeding effect Effects 0.000 abstract description 8
- 230000009467 reduction Effects 0.000 abstract description 7
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 6
- 230000029087 digestion Effects 0.000 abstract description 6
- 230000008569 process Effects 0.000 abstract description 5
- 108010059892 Cellulase Proteins 0.000 abstract description 4
- 108091005804 Peptidases Proteins 0.000 abstract description 4
- 239000004365 Protease Substances 0.000 abstract description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract description 4
- 241000191967 Staphylococcus aureus Species 0.000 abstract description 4
- 229940106157 cellulase Drugs 0.000 abstract description 4
- 230000036039 immunity Effects 0.000 abstract description 4
- 239000004382 Amylase Substances 0.000 abstract description 3
- 102000013142 Amylases Human genes 0.000 abstract description 3
- 108010065511 Amylases Proteins 0.000 abstract description 3
- 241000588724 Escherichia coli Species 0.000 abstract description 3
- 241000191938 Micrococcus luteus Species 0.000 abstract description 3
- 241000607142 Salmonella Species 0.000 abstract description 3
- 235000019418 amylase Nutrition 0.000 abstract description 3
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 abstract description 3
- 235000015921 sodium selenite Nutrition 0.000 abstract description 3
- 229960001471 sodium selenite Drugs 0.000 abstract description 2
- 239000011781 sodium selenite Substances 0.000 abstract description 2
- 238000004064 recycling Methods 0.000 abstract 1
- 235000013594 poultry meat Nutrition 0.000 description 24
- 238000002474 experimental method Methods 0.000 description 21
- 239000000047 product Substances 0.000 description 16
- 238000012360 testing method Methods 0.000 description 14
- 241000726221 Gemma Species 0.000 description 12
- 235000013601 eggs Nutrition 0.000 description 12
- 239000000243 solution Substances 0.000 description 10
- 230000000968 intestinal effect Effects 0.000 description 9
- 239000006228 supernatant Substances 0.000 description 9
- 235000014590 basal diet Nutrition 0.000 description 8
- 201000010099 disease Diseases 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 230000004083 survival effect Effects 0.000 description 7
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 230000036541 health Effects 0.000 description 5
- 244000005700 microbiome Species 0.000 description 5
- 235000015097 nutrients Nutrition 0.000 description 5
- 210000002784 stomach Anatomy 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 241000287828 Gallus gallus Species 0.000 description 4
- 239000003674 animal food additive Substances 0.000 description 4
- 230000003115 biocidal effect Effects 0.000 description 4
- 235000013330 chicken meat Nutrition 0.000 description 4
- 230000001079 digestive effect Effects 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 229940088598 enzyme Drugs 0.000 description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 4
- 235000013406 prebiotics Nutrition 0.000 description 4
- 244000025254 Cannabis sativa Species 0.000 description 3
- 229910021529 ammonia Inorganic materials 0.000 description 3
- 230000003385 bacteriostatic effect Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000003862 health status Effects 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 238000012549 training Methods 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 108020004465 16S ribosomal RNA Proteins 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- 206010012735 Diarrhoea Diseases 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 239000006142 Luria-Bertani Agar Substances 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 241001148470 aerobic bacillus Species 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 239000012876 carrier material Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000002550 fecal effect Effects 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 235000014655 lactic acid Nutrition 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- 238000009938 salting Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 230000001228 trophic effect Effects 0.000 description 2
- 241000186000 Bifidobacterium Species 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 102000016918 Complement C3 Human genes 0.000 description 1
- 108010028780 Complement C3 Proteins 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 239000012267 brine Substances 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- IQFVPQOLBLOTPF-HKXUKFGYSA-L congo red Chemical compound [Na+].[Na+].C1=CC=CC2=C(N)C(/N=N/C3=CC=C(C=C3)C3=CC=C(C=C3)/N=N/C3=C(C4=CC=CC=C4C(=C3)S([O-])(=O)=O)N)=CC(S([O-])(=O)=O)=C21 IQFVPQOLBLOTPF-HKXUKFGYSA-L 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000013872 defecation Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000036750 egg laying performance Effects 0.000 description 1
- 210000003278 egg shell Anatomy 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000000369 enteropathogenic effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000001408 fungistatic effect Effects 0.000 description 1
- 210000000232 gallbladder Anatomy 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 210000004051 gastric juice Anatomy 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 108010090785 inulinase Proteins 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 1
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
Landscapes
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (3)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310719942.0A CN103981118B (zh) | 2013-12-24 | 2013-12-24 | 一种枯草芽孢杆菌饲料添加剂及其制备方法和应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310719942.0A CN103981118B (zh) | 2013-12-24 | 2013-12-24 | 一种枯草芽孢杆菌饲料添加剂及其制备方法和应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN103981118A CN103981118A (zh) | 2014-08-13 |
CN103981118B true CN103981118B (zh) | 2017-11-03 |
Family
ID=51273288
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201310719942.0A Active CN103981118B (zh) | 2013-12-24 | 2013-12-24 | 一种枯草芽孢杆菌饲料添加剂及其制备方法和应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN103981118B (zh) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104738308A (zh) * | 2015-04-20 | 2015-07-01 | 赵德邦 | 一种有效提高饲料利用率的牲畜饲料添加剂及其制备工艺 |
CN105400729A (zh) * | 2015-07-16 | 2016-03-16 | 山东省食品发酵工业研究设计院 | 一种抑菌性产木聚糖酶的枯草芽孢杆菌菌株 |
CN105368757A (zh) * | 2015-12-15 | 2016-03-02 | 湖南农业大学 | 一株猪舍粪尿环境适应型枯草芽孢杆菌及其应用 |
CN106399169A (zh) * | 2016-09-14 | 2017-02-15 | 广东省微生物研究所 | 枯草芽孢杆菌1.1122在制备淀粉酶、抗细菌药物和饲料添加剂中的应用 |
CN106190933B (zh) * | 2016-09-28 | 2019-08-13 | 吉林省农业科学院 | 广谱抗致病菌的枯草芽孢杆菌及其应用 |
CN107058181B (zh) * | 2017-04-13 | 2020-07-28 | 中国农业科学院特产研究所 | 一种枯草芽孢杆菌及其分离方法与应用 |
CN109337841B (zh) * | 2017-12-27 | 2021-01-12 | 山东农业大学 | 一株具有高效抑菌性能的枯草芽孢杆菌bys2 |
CN108587957B (zh) * | 2018-04-25 | 2020-05-29 | 华中农业大学 | 一株高产复合酶的益生地衣芽孢杆菌的筛选及应用 |
CN109161509B (zh) * | 2018-10-10 | 2021-09-03 | 中国农业科学院兰州兽医研究所 | 一株能防治牛羊腹泻病的菌株 |
KR102299266B1 (ko) * | 2019-03-07 | 2021-09-08 | 씨제이제일제당 주식회사 | 바실러스 서브틸리스 cjbs303 및 이를 포함하는 조성물 |
CN112725222B (zh) * | 2020-12-21 | 2023-01-31 | 齐鲁工业大学 | 一株产复合酶枯草芽孢杆菌q3、培养方法及应用 |
CN112760251A (zh) * | 2021-01-05 | 2021-05-07 | 安徽农业大学 | 一种复合型芽孢杆菌益生菌及其制备与应用 |
CN113549575B (zh) * | 2021-07-20 | 2022-09-23 | 河北农业大学 | 一种高产蛋白酶的枯草芽孢杆菌fm-20菌株及其应用 |
CN113862196B (zh) * | 2021-11-03 | 2023-09-08 | 中谷生物科技(大连)有限公司 | 一株枯草芽孢杆菌sd-kc-001及其应用 |
CN114032200A (zh) * | 2021-12-10 | 2022-02-11 | 安徽天富来生物科技有限公司 | 一种养殖动物的枯草芽孢杆菌饲料添加剂的制备方法 |
CN114107432B (zh) * | 2022-01-27 | 2022-05-27 | 广州傲农生物科技有限公司 | 枯草芽孢杆菌活菌计数培养基、稀释液及活菌计数方法 |
CN114874940B (zh) * | 2022-05-10 | 2023-07-14 | 吉林省农业科学院 | 一种复合菌剂及其发酵物和应用 |
CN116004757A (zh) * | 2023-02-16 | 2023-04-25 | 青岛农业大学 | 一种幼龄反刍动物替抗添加剂及其筛选方法 |
CN117106676B (zh) * | 2023-10-24 | 2024-02-27 | 北京大北农科技集团股份有限公司 | 一株枯草芽孢杆菌及其在饲料生产中的应用 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101822321B (zh) * | 2010-03-19 | 2012-11-28 | 计成 | 枯草芽孢杆菌ansb060的应用 |
CN102178057B (zh) * | 2011-05-06 | 2013-03-27 | 北京大北农科技集团股份有限公司 | 枯草芽孢杆菌及其饲料添加剂和发酵剂 |
CN103060222A (zh) * | 2012-09-19 | 2013-04-24 | 中国农业科学院饲料研究所 | 具有益生作用的枯草芽孢杆菌b27及其应用 |
CN103289915A (zh) * | 2013-01-29 | 2013-09-11 | 广州格拉姆生物科技有限公司 | 一种稳定安全的枯草芽孢杆菌制剂及其制备方法和用途 |
-
2013
- 2013-12-24 CN CN201310719942.0A patent/CN103981118B/zh active Active
Also Published As
Publication number | Publication date |
---|---|
CN103981118A (zh) | 2014-08-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103981118B (zh) | 一种枯草芽孢杆菌饲料添加剂及其制备方法和应用 | |
CN106011036B (zh) | 一株具有益生作用的凝结芽孢杆菌hew-b379及其应用 | |
CN101638627B (zh) | 一株枯草芽孢杆菌及其在生物饲料添加剂中的应用 | |
CN102409015B (zh) | 一种复合微生态制剂及其在饲料添加剂中的应用和预混料 | |
CN104293696B (zh) | 一株粪肠球菌hew-a131及其应用 | |
CN101580799B (zh) | 一种复合微生态制剂及其应用 | |
CN103275907B (zh) | 一种解淀粉芽胞杆菌及制备方法和应用 | |
CN102199590B (zh) | 一种微胶囊化增殖培养制备酪酸菌菌粉的方法及其应用 | |
CN101671638B (zh) | 一种双歧杆菌新菌株及其发酵制备方法与应用 | |
CN103981115A (zh) | 一株高抗逆性屎肠球菌及其应用 | |
CN102559539B (zh) | 嗜酸乳杆菌及其应用与饲料添加剂和预混料 | |
CN106906154A (zh) | 一株植物乳杆菌菌株及其饲料添加剂与饲料 | |
CN101971920B (zh) | 猪源罗伊氏乳酸杆菌冻干制剂及其制备方法 | |
CN104480052A (zh) | 一种含三株菌的复合芽孢杆菌制剂及其制备方法和在生态养殖中的应用 | |
CN107868764A (zh) | 一种增强宠物免疫力的复合益生菌粉制剂及其制备方法 | |
CN102028110A (zh) | 一种肉鸡饲用的益生菌-黄芪多糖组合物 | |
CN102391962B (zh) | 一种屎肠球菌制剂及其生产工艺 | |
CN103289910A (zh) | 一种凝结芽孢杆菌的固体发酵生产方法 | |
CN109161509A (zh) | 一株能防治牛羊腹泻病的菌株 | |
CN109593666A (zh) | 一种复合微生态制剂及其制备方法和应用 | |
CN107177522A (zh) | 一株高活性饲用植物乳杆菌及其培养方法和应用 | |
CN110982753A (zh) | 凝结芽孢杆菌mes847、菌剂、鸡饲料、制备方法及应用 | |
CN107821789A (zh) | 一种提高鱼虾肠道健康度的生物酵素及其制备方法和应用 | |
CN106906166A (zh) | 一种猪源唾液乳杆菌活菌制剂及应用 | |
CN101904411B (zh) | 一种复合微生态制剂及其应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB02 | Change of applicant information |
Address after: 100085 Beijing city Haidian District Third Street No. 9 D803 Applicant after: Beijing WIK biotechnology Limited by Share Ltd Applicant after: Shenyang Vica Animal Husbandry Technology Co., Ltd. Address before: 100085 Beijing city Haidian District Third Street No. 9 D803 Applicant before: Beijing Vica Group Co., Ltd. Applicant before: Shenyang Vica Animal Husbandry Technology Co., Ltd. |
|
COR | Change of bibliographic data | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20190430 Address after: 803, Block D, Jiahua Building, 9 Shangdi Third Street, Haidian District, Beijing 100085 Co-patentee after: SHENYANG VICA ANIMAL HUSBANDRY CO., LTD. Patentee after: Beijing WIK biotechnology Limited by Share Ltd Co-patentee after: Cangzhou Weijia Animal Husbandry Co., Ltd. Address before: 100085 D803 9, Shang di San Jie, Haidian District, Beijing. Co-patentee before: Shenyang Vica Animal Husbandry Technology Co., Ltd. Patentee before: Beijing WIK biotechnology Limited by Share Ltd |
|
CI03 | Correction of invention patent | ||
CI03 | Correction of invention patent |
Correction item: Patentee|Address|Patentee Correct: Beijing daweijia Biotechnology Co., Ltd|100085 803, block D, Jiahua building, No. 9, Shangdi Third Street, Haidian District, Beijing|Shenyang Weijia animal husbandry technology Co., Ltd.; Cangzhou Weijia animal husbandry Co., Ltd False: Beijing daweijia Biotechnology Co., Ltd|100085 803, block D, Jiahua building, No. 9, Shangdi Third Street, Haidian District, Beijing|Shenyang Weijia animal husbandry Co., Ltd.; Cangzhou Weijia animal husbandry Co., Ltd Number: 21-01 Volume: 35 |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20201202 Address after: No.22, Zhenxing Road, offshore economic zone, Shenyang, Liaoning Province, 110206 Patentee after: SHENYANG VICA ANIMAL HUSBANDRY TECHNOLOGY Co.,Ltd. Patentee after: CANGZHOU WEIJI ANIMAL HUSBANDRY Co.,Ltd. Address before: 100085, D, building 9, Ka Wah building, 803 3rd Street, Beijing, Haidian District Patentee before: BEIJING VICA BIOTECHNOLOGY Co.,Ltd. Patentee before: SHENYANG VICA ANIMAL HUSBANDRY TECHNOLOGY Co.,Ltd. Patentee before: CANGZHOU WEIJI ANIMAL HUSBANDRY Co.,Ltd. |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20210901 Address after: 110206 No. 22, Zhenxing Road, Shenyang offshore economic zone, Shenyang City, Liaoning Province Patentee after: SHENYANG VICA ANIMAL HUSBANDRY TECHNOLOGY Co.,Ltd. Patentee after: CANGZHOU WEIJI ANIMAL HUSBANDRY Co.,Ltd. Patentee after: Shenyang Weijia Biotechnology Co.,Ltd. Address before: No. 22, Zhenxing Road, offshore economic zone, Shenyang, Liaoning, 110206 Patentee before: SHENYANG VICA ANIMAL HUSBANDRY TECHNOLOGY Co.,Ltd. Patentee before: CANGZHOU WEIJI ANIMAL HUSBANDRY Co.,Ltd. |