CN103947588A - Breeding method for Crassostrea sikamea by taking left shell radial ridge number as mark - Google Patents
Breeding method for Crassostrea sikamea by taking left shell radial ridge number as mark Download PDFInfo
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- CN103947588A CN103947588A CN201410153760.6A CN201410153760A CN103947588A CN 103947588 A CN103947588 A CN 103947588A CN 201410153760 A CN201410153760 A CN 201410153760A CN 103947588 A CN103947588 A CN 103947588A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a breeding method for Crassostrea sikamea by taking the left shell radial ridge number as a mark. The method comprises the steps of a, strain dividing: dividing the Crassostrea sikamea into three groups according to the left shell radial ridge number of the Crassostrea sikamea, wherein the three groups comprise a ridge-free strain without a radial ridge, a less-ridge strain with the number of the radial ridges being smaller than or equal to 5 and a multi-ridge strain with the radial ridge number being larger than 6; b, strain self multiplying: taking the 3 strain individuals in the step a as parent samples, performing offspring multiplying by group self multiplying in the strains and then performing normal cultivating; c, strain purifying; taking the strains cultivated in the step b as the parent samples, eliminating the parent sample individuals with the left shell radial ridge number which is not consistent with the strain standards, repeating the group self multiplying in the strain for a plurality of times for offspring multiplying and performing normal cultivating, and performing oriented purifying to obtain a new strain with relatively stable characters. The appearance of the new multi-ridge and less-ridge strains obtained by the method is tidier than that of the traditionally cultivated Crassostrea sikamea, and the yield can be improved by 15-30%.
Description
Technical field:
The invention belongs to shellfish genetic breeding field in modern marine agricultural, be specifically related to a kind of taking left shell radiation ridge number as mark
The Kumamoto oyster producing method for seed of note.
Background technology:
Kumamoto oyster Crassostrea sikamea originates in East Asia, be distributed in Japan, Korea S and on the south China Zhejiang, the ground such as Guangdong, Guangxi, Hainan, be wild species, hypersaline environment, i.e. partially salty region in degree of saltiness aqua region in hobby high temperature, the main distribution place of production concentrates on bay and estuary region, belongs to the economic kind of subtropics oyster.In China, it is the economic kind of the important oyster of the 4th class being only second to after Hong Kong oyster, Portuguese oyster, long oyster, and annual production, 100,000 tons of left and right, is wild adopting and catches.
Because Kumamoto oyster meat taste is better, be 2 times of long oyster in its price of the U.S., suitable in China and long oyster, Portuguese oyster price, lower than Hong Kong oyster commercial price.Chinese Academy of Science Nanhai Ocean Research Institute explain sub-ox subject group by parent's accelerating, the hatching of hastening parturition, larvae cultivation, juvenile mollusk be bred as, young shellfish is formed etc., and technological means is cultivated shiploads of merchandise specification Kumamoto oyster in China.
So far, except the relevant reports such as the geographical distribution of Kumamoto oyster, artificial breeding, all there is not yet its genetic breeding correlative study both at home and abroad.
Summary of the invention:
The object of the invention is for the present situation that there is no high yield, new quality variety in current Kumamoto oyster culture industry, a kind of Kumamoto oyster producing method for seed taking left shell radiation ridge number as mark is provided.Can obtain by the method the many ridges of Kumamoto oyster, the few ridge new lines that high yield, high-quality can genetic stabilities.
Inventor's discovery, the right shell of Kumamoto oyster is almost straightened condition, and most of left shell there will be several radiation ridges, its number has larger variability.For this reason, inventor is how many according to the left shell radiation of Kumamoto oyster ridge number, is divided into without ridge strain (radiation ridge=0), few ridge strain (radiation ridge is for≤5) and many ridges strain (6 of radiation ridge >).So, between different numbers radiation ridge strains, whether have phenotypic difference, can the new lines that utilize the correlation of quantitative character and economic characters to filter out high yield, high-quality become crucial innovative point of the present invention.
Kumamoto oyster producing method for seed taking left shell radiation ridge number as mark of the present invention, is characterized in that, comprises the following steps:
A, strain divide: according to the left shell radiation ridge number of Kumamoto oyster, be located as 3 large monoids, be respectively without ridge strain, its radiation ridge=0, few ridge strain, its radiation ridge be≤5 with many ridges strain, it radiates 6 of ridge >;
B, strain are from numerous: using 3 strain individualities in a step as parent, carried out filial generation and breed from numerous by the colony in strain, carry out afterwards routine and form;
C, strain purifying: using the strain of forming in b step as parent, weed out parent's individuality that left shell radiation ridge number does not meet strain standard, colony in repeated several times strain carries out filial generation and breeds with routine and form from numerous, carry out directed purifying, can the relatively stable new lines of acquired character.
Described filial generation is bred can be by dissecting or the mode of artificial induced spawning be carried out filial generation and bred.
Described strain purifying can be undertaken by family selective breeding or colony's seed selection.
Described several times are preferably 1-3 time.
During described a step strain is divided, first need to weed out the left shell radiation ridge number proterties individuality that is not true to type, select afterwards left shell radiation ridge number proterties typical individual to carry out strain division.
Not affected by environment in order to ensure Morphology of shell, left shell radiation ridge number gives full expression to, and all adopts monomer aquaculture model to carry out Screening of Strains Against.
The present invention is in conjunction with the feature of the left shell radiation of the Kumamoto oyster ridge number property of there are differences, be located as 3 large monoids, be respectively without ridge strain (radiation ridge=0), few ridge strain (radiation ridge is for≤5) and many ridges strain (6 of radiation ridge >); By strain divide, strain is from technological means such as numerous, strain purifying, according to the relevance principle of radiation ridge quantity and growth traits, realize the collaborative selection of quantitative character and economic characters, obtained many ridges, few ridge new lines that high yield, high-quality can genetic stabilities.The present invention obtains many ridges, few ridge new lines is more neat in appearance than the Kumamoto oyster of traditional cultivation, and its output can improve 15-30% simultaneously, and this is that solid practical basis has been established in Kumamoto oyster new lines (kind) exploitation and cultured output raising.That the present invention has is practical, be easy to the advantages such as popularization.
Brief description of the drawings:
Fig. 1 is 3 Kumamoto oyster new lines schematic diagrames of the present invention.
Embodiment:
Following examples are to further illustrate of the present invention, instead of limitation of the present invention.
Embodiment 1
A, strain divide: on April 12nd, 2012, in Kumamoto oyster mating season, using slope, Zhanjiang, a wild Kumamoto oyster, as basic population, carries out first filial generation in marine economic animal experiment station, Zhanjiang of Chinese Academy of Science Nanhai Ocean Research Institute and breeds, forms.On April 10th, 2013, first filial generation gonad maturity, randomly draw 3000 individualities, according to left shell radiation ridge number, be located as 3 large monoids, weed out proterties and be not true to type after individual 2232, filter out without 216 of 245 of ridge strains (radiation ridge=0), 307 of few ridge strains (radiation ridge is≤5) and many ridges strains (6 of radiation ridge >).
B, strain are from numerous: using 3 strain individualities in a step as parent, on April 10th, 2014, carry out second filial breed (can by dissect or the mode of artificial induced spawning be carried out) by the colony in strain from numerous form, carry out afterwards conventional forming.Wherein.As shown in table 1, in each strain individuality in continuous two generations, with many ridges strain individuality large (fresh weight maximum, the high shell length that is slightly larger than of shell), few ridge strain secondly, relatively little without ridge strain.
C, strain purifying: using the second filial of 3 strains in b step as parent, weed out parent's individuality that left shell radiation ridge number does not meet above-mentioned 3 strain criteria for classifying, carrying out second filial through the colony in 1-3 strain from numerous form breeds, carry out afterwards conventional forming, carry out directed purifying, can obtain that phenotypic character is relatively stable, radiation ridge number relatively consistent Kumamoto oyster new lines.
Table 1: the cultivation parameter of different radiation ridge number Kumamoto oyster strain continuous 2 generations (F1, F2) 360 ages in days
Claims (4)
1. the Kumamoto oyster producing method for seed taking left shell radiation ridge number as mark, is characterized in that, comprises the following steps:
A, strain divide: according to the left shell radiation ridge number of Kumamoto oyster, be located as 3 large monoids, be respectively without ridge strain, its radiation ridge=0, few ridge strain, its radiation ridge be≤5 with many ridges strain, it radiates 6 of ridge >;
B, strain are from numerous: using 3 strain individualities in a step as parent, carried out filial generation and breed from numerous by the colony in strain, carry out afterwards routine and form;
C, strain purifying: using the strain of forming in b step as parent, weed out parent's individuality that left shell radiation ridge number does not meet strain standard, colony in repeated several times strain carries out filial generation and breeds with routine and form from numerous, carries out directed purifying, can the relatively stable new lines of acquired character.
2. Kumamoto oyster producing method for seed according to claim 1, is characterized in that, it is to be undertaken by monomer cultivate mode that described filial generation is cultivated, to guarantee to obtain the natural Morphology of shell of Kumamoto oyster.
3. Kumamoto oyster producing method for seed according to claim 1, is characterized in that, described strain purifying can be undertaken by family selective breeding or colony's seed selection.
4. Kumamoto oyster producing method for seed according to claim 1, is characterized in that, described several times are 1-3 time.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410153760.6A CN103947588B (en) | 2014-04-16 | 2014-04-16 | A kind of is the Kumamoto oyster producing method for seed marked with left shell radiation ridge number |
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| CN201410153760.6A CN103947588B (en) | 2014-04-16 | 2014-04-16 | A kind of is the Kumamoto oyster producing method for seed marked with left shell radiation ridge number |
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| CN103947588A true CN103947588A (en) | 2014-07-30 |
| CN103947588B CN103947588B (en) | 2015-09-02 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104365515A (en) * | 2014-11-25 | 2015-02-25 | 鲁东大学 | Breeding method of crassostrea gigas with high melanin content in soft parts |
Citations (5)
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| CN101288388A (en) * | 2008-06-11 | 2008-10-22 | 大连水产学院 | Choice method suitable for seashell rapid growing |
| CN101317554A (en) * | 2008-06-11 | 2008-12-10 | 大连水产学院 | Hybrid seeds preparation of Ruditapes philippinarum with radial rib as mark |
| CN102742531A (en) * | 2012-07-31 | 2012-10-24 | 中国海洋大学 | Construction method of superior strains of rapid-growing crassostrea gigas |
| CN102742532A (en) * | 2012-07-31 | 2012-10-24 | 中国海洋大学 | Cultivation method of novel strain of crassostrea gigas with pure purple left shells |
| CN102763617A (en) * | 2012-08-20 | 2012-11-07 | 中国海洋大学 | Artificial breeding method of sikamea oysters |
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2014
- 2014-04-16 CN CN201410153760.6A patent/CN103947588B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101288388A (en) * | 2008-06-11 | 2008-10-22 | 大连水产学院 | Choice method suitable for seashell rapid growing |
| CN101317554A (en) * | 2008-06-11 | 2008-12-10 | 大连水产学院 | Hybrid seeds preparation of Ruditapes philippinarum with radial rib as mark |
| CN102742531A (en) * | 2012-07-31 | 2012-10-24 | 中国海洋大学 | Construction method of superior strains of rapid-growing crassostrea gigas |
| CN102742532A (en) * | 2012-07-31 | 2012-10-24 | 中国海洋大学 | Cultivation method of novel strain of crassostrea gigas with pure purple left shells |
| CN102763617A (en) * | 2012-08-20 | 2012-11-07 | 中国海洋大学 | Artificial breeding method of sikamea oysters |
Non-Patent Citations (3)
| Title |
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| 李石磊等: "单体牡蛎苗种培育技术研究进展", 《北京水产》 * |
| 滕爽爽等: "长牡蛎（Crassostrea gigas）与熊本牡蛎（C. sikamea）杂交的受精细胞学观察及子一代的生长比较", 《海洋与湖沼》 * |
| 王辉等: "南海毛蚶形态特征对体重的相关分析", 《热带海洋学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104365515A (en) * | 2014-11-25 | 2015-02-25 | 鲁东大学 | Breeding method of crassostrea gigas with high melanin content in soft parts |
| CN104365515B (en) * | 2014-11-25 | 2016-07-13 | 鲁东大学 | A kind of selection of the soft body long Concha Ostreae of height melanin content |
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| CN103947588B (en) | 2015-09-02 |
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