CN102742531A - Construction method of superior strains of rapid-growing crassostrea gigas - Google Patents
Construction method of superior strains of rapid-growing crassostrea gigas Download PDFInfo
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- CN102742531A CN102742531A CN2012102693392A CN201210269339A CN102742531A CN 102742531 A CN102742531 A CN 102742531A CN 2012102693392 A CN2012102693392 A CN 2012102693392A CN 201210269339 A CN201210269339 A CN 201210269339A CN 102742531 A CN102742531 A CN 102742531A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
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Abstract
The invention discloses a construction method of superior strains of rapid-growing crassostrea gigas. The construction method is characterized by comprising the following steps: A. building a breeding base population, using the shell height and the total weight as a seed selection goal, and keeping and collecting parent shellfish according to high-selective intensity as the basic parent shellfish population; B. by adopting the population continuous progeny selection, selecting a certain number of gonadal matured individuals from the preceding generation of parent shellfish population to form a succeeding generation parent shellfish population, conducting artificial insemination by adopting a post-mortem method; at the pre-maturation period, keeping and collecting the individuals with high shellfish height and high total weight according to the high selective intensity as the parent shellfish for breeding; C, conducting the quantitative character detection and the genetic parameter estimation on the succeeding generation parent shellfish population in step B; D, repeating steps B and C while performing the genetic diversity analysis on three generations of the continuous progeny selection systems, and conducting the evaluation of the breeding effect. With the adoption of the construction method of the superior strains of the rapid-growing crassostrea gigas, the breeding progress is largely speeded up, the labor cost of breeding is reduced, the breeding effect is obvious, and the economic benefit of breeding the crassostrea gigas is improved.
Description
Technical field
The invention belongs to aquatic products genetic breeding technology, specifically, relate to the construction method of the long oyster excellent strain of a kind of quick growth.
Background technology
Long oyster (Crassostrea gigas); Originate in Japan, China and Korea S area; Having advantages such as environmental adaptation is strong, growth is fast, local flavor is delicious, nutritious, is to culture economic shellfish widest in area, that output is the highest in the world, also is the most important a kind of mariculture kind of China.At present, China head oyster place of production mainly concentrates on coastal areas such as Shandong, Liaoning, Zhejiang, Fujian, Guangdong, and annual production reaches millions of tons, has obtained remarkable economic efficiency and social benefit.Because the long oyster quality that China cultures is low, specification is poor, can't get into international mainstream market, therefore, cultivate that quality better, growth are fast, the fine quality of strong stress resistance, be the effective way of raising China oyster product quality.
Summary of the invention
The technological problems that the present invention will solve provides the construction method of the long oyster excellent strain of a kind of quick growth, and the present invention can accelerate breeding process greatly, reduces seed selection labour cost, and breeding effect is obvious, has effectively avoided the inbreeding depression phenomenon simultaneously.The present invention simultaneously also can be used for long oyster culture rearing new variety, solves the slow problem of long oyster growth, can improve the economic benefit of long oyster culture.
For addressing the above problem, the technical scheme that the present invention adopted is:
The construction method of the long oyster excellent strain of a kind of quick growth is characterized in that:
Comprise following steps:
A, the wild seed of the long oyster of introduction Japan are set up the breeding basic population, serve as the seed selection target with shell height and gross weight, leave and take close shellfish with high selection intensity, as the close shellfish crowd in basis;
B, employing colony subculture selection, the gonad maturity of from previous generation parent shellfish crowd, selecting some is individual, forms subculture parent shellfish crowd, and the employing post-mortem method is carried out artificial insemination; The prematuration period, leave and take high high, the total heavy individuality of shell as the close shellfish of breeding with high selection intensity;
C, mensuration and genetic parameter that the subculture among step B parent shellfish crowd is carried out quantitative character are estimated;
D, repeating step B and C select close shellfish crowd and carry out growth traits without the shellfish crowd control group of seed selection relatively through subculture, use molecule labelling method to three generations's subculture breeding line analysis of genetic diversity simultaneously, carry out the seed selection recruitment evaluation.
Say further:
The wild seed of the long oyster of said Japan, it is tall and big in 138cm to choose shell, and the individuality that average shell height is 143cm is as close shellfish, and selection intensity is 1.7, and formation base parent shellfish crowd.
Said colony subculture selection; Be from alternative close shellfish crowd of each generation, to select 80-100 gonad maturity individuality respectively; And female, male each 40-50, to dissect the clip sexual gland and carry out artificial insemination, the while is with the selection intensity of 1.7-1.9; Each for the prematuration period to leave and take shell high long, the big individuality of body weight is as the close shellfish of breeding.
Further say:
The subculture that obtains among said step D parent shellfish crowd carries out the high comparison with gross weight of shell with specific control group, uses the analysis of genetic diversity of 10 microsatellite markers to the close shellfish crowd of subculture that obtains among the step D simultaneously, carries out the seed selection recruitment evaluation.
Said 10 high microsatellite markers of long oyster polymorphism, i.e. ucdCg-129, ucdCg-130, ucdCg-134, ucdCg-138; UcdCg-148, ucdCg-149, ucdCg-151, ucdCg-160; UcdCg-198, ucdCg-200, and, carry out analysis of genetic diversity through the PCR reaction system.
Further say:
Said PCR reaction system is 10 μ L systems, comprises 100ng template DNA, 1 * PCR buffer, 0.2mM dNTP mixed liquor, 1 μ M primer, 1-2mM MgCl
2With 0.25U Taq archaeal dna polymerase.The PCR reaction condition is the preparatory sex change 3min of 94oC; 35 circulations are 94oC 1min, annealing 1min, 72oC 1min; 72oC extends 5min.
Owing to adopted technique scheme, compared with prior art, the present invention can accelerate breeding process greatly, reduces seed selection labour cost, and breeding effect is obvious, solves the slow problem of long oyster growth, can improve the economic benefit of long oyster culture.Simultaneously, the present invention is the stock of colony's seed selection with the abundant wild seed of Japan head oyster of genetic variation, helps keeping its genetic diversity, selects breeding for colony's subculture and leaves very big space.And the close shellfish individual amount of in implementation process, leaving and taking is many, and the female-male proportion balance has effectively been avoided the inbreeding depression phenomenon.
Embodiment
Embodiment:
The construction method of the long oyster excellent strain of a kind of quick growth comprises following steps:
A, the wild seed of the long oyster of introduction Japan are set up the breeding basic population, serve as the seed selection target with shell height and gross weight, leave and take close shellfish with high selection intensity, as the close shellfish crowd in basis.
B, from previous generation parent shellfish crowd, adopt colony's subculture selection, the gonad maturity of from previous generation parent shellfish crowd, selecting some is individual, forms subculture parent shellfish crowd, adopts post-mortem method to carry out artificial insemination; The prematuration period, leave and take high high, the total heavy individuality of shell as the close shellfish of breeding with high selection intensity.
C, mensuration and genetic parameter that the subculture among step B parent shellfish crowd is carried out quantitative character are estimated;
D, repeating step B and C select close shellfish crowd and carry out growth traits without the shellfish crowd control group of seed selection relatively through subculture, use molecule labelling method to three generations's subculture breeding line analysis of genetic diversity simultaneously, carry out the seed selection recruitment evaluation.
In the present embodiment, the wild seed of the long oyster of said Japan, it is tall and big in 138cm to choose shell, and the individuality that average shell height is 143cm is as close shellfish, and selection intensity is 1.7, and forms generation parent shellfish crowd.
Said colony subculture selection; Be from alternative close shellfish crowd of each generation, to select 80-100 gonad maturity individuality respectively; And female, male each 40-50, to dissect the clip sexual gland and carry out artificial insemination, the while is with the selection intensity of 1.7-1.9; Each for the prematuration period to leave and take shell high long, the big individuality of body weight is as the close shellfish of breeding.
In addition; In the present embodiment, the subculture parent shellfish crowd who obtains among the said step D carries out the comparison of shell height and gross weight with specific control group; Use the analysis of genetic diversity of 10 microsatellite markers simultaneously, carry out the seed selection recruitment evaluation the subculture that obtains among step D parent shellfish crowd.
Generally, 10 high microsatellite markers of long oyster polymorphism, i.e. ucdCg-129, ucdCg-130; UcdCg-134, ucdCg-138, ucdCg-148, ucdCg-149; UcdCg-151, ucdCg-160, ucdCg-198; UcdCg-200, and, carry out analysis of genetic diversity through the PCR reaction system.The PCR reaction system is 10 μ L systems, comprises 100ng template DNA, 1 * PCR buffer, 0.2mM dNTP mixed liquor, 1 μ M primer, 1-2mM MgCl
2With 0.25U Taq archaeal dna polymerase.The PCR reaction condition is the preparatory sex change 3min of 94oC; 35 circulations are 94oC 1min, annealing 1min, 72oC 1min; 72oC extends 5min.
Should be noted that at last; Above content is only in order to explain technical scheme of the present invention; But not to the restriction of protection domain of the present invention; The simple modification that those of ordinary skill in the art carries out technical scheme of the present invention perhaps is equal to replacement, does not all break away from the essence and the scope of technical scheme of the present invention.
Claims (6)
1. quick a growth grown the construction method of oyster excellent strain, it is characterized in that:
Comprise following steps:
A, the wild seed of the long oyster of introduction Japan are set up the breeding basic population, serve as the seed selection target with shell height and gross weight, leave and take close shellfish with high selection intensity, as the close shellfish crowd in basis;
B, employing colony subculture selection, the gonad maturity of from previous generation parent shellfish crowd, selecting some is individual, forms subculture parent shellfish crowd, and the employing post-mortem method is carried out artificial insemination; The prematuration period, leave and take high high, the total heavy individuality of shell as the close shellfish of breeding with high selection intensity;
C, mensuration and genetic parameter that the subculture among step B parent shellfish crowd is carried out quantitative character are estimated;
D, repeating step B and C select close shellfish crowd and carry out growth traits without the shellfish crowd control group of seed selection relatively through subculture, use molecule labelling method to three generations's subculture breeding line analysis of genetic diversity simultaneously, carry out the seed selection recruitment evaluation.
2. the construction method of the long oyster excellent strain of quick growth according to claim 1; It is characterized in that: the wild seed of the long oyster of said Japan, it is tall and big in 138cm to choose shell, and the individuality that average shell height is 143cm is as close shellfish; Selection intensity is 1.7, and formation base parent shellfish crowd.
3. the construction method of the long oyster excellent strain of a kind of quick growth according to claim 1; It is characterized in that: said colony subculture selection is from alternative close shellfish crowd of each generation, to select 80-100 gonad maturity individuality respectively, and female, male each 40-50; Dissect the clip sexual gland and carry out artificial insemination; Simultaneously with the selection intensity of 1.7-1.9, each for the prematuration period to leave and take shell high long, the big individuality of body weight is as the close shellfish of breeding.
4. the construction method of the long oyster excellent strain of a kind of quick growth according to claim 1; It is characterized in that: the subculture parent shellfish crowd who obtains among the said step D; Carry out the comparison of shell height and gross weight with specific control group; Use the analysis of genetic diversity of 10 microsatellite markers simultaneously, carry out the seed selection recruitment evaluation the subculture that obtains among step D parent shellfish crowd.
5. the construction method of the long oyster excellent strain of quick growth according to claim 4 is characterized in that: said 10 high microsatellite markers of long oyster polymorphism, i.e. ucdCg-129, ucdCg-130; UcdCg-134, ucdCg-138, ucdCg-148, ucdCg-149; UcdCg-151, ucdCg-160, ucdCg-198; UcdCg-200, and, carry out analysis of genetic diversity through the PCR reaction system.
6. the construction method of the long oyster excellent strain of quick growth according to claim 5; It is characterized in that: said PCR reaction system is 10 μ L systems, comprises 100ng template DNA, 1 * PCR buffer, 0.2mM dNTP mixed liquor, 1 μ M primer, 1-2mM MgCl
2With 0.25U Taq archaeal dna polymerase.The PCR reaction condition is the preparatory sex change 3min of 94oC; 35 circulations are 94oC 1min, annealing 1min, 72oC 1min; 72oC extends 5min.
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Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103719000A (en) * | 2013-12-20 | 2014-04-16 | 中国科学院南海海洋研究所 | Fertility recovery method for oyster distant hybrid sterility |
| CN103798166A (en) * | 2014-01-24 | 2014-05-21 | 中国科学院南海海洋研究所 | Method for large-scale indoor artificial breeding of Hong Kong oysters in coastal region of South China |
| CN103814848A (en) * | 2014-02-28 | 2014-05-28 | 中国科学院南海海洋研究所 | Breeding method for new line of hybrid Crassostrea hongkongensis and Crassostrea gigas |
| CN103891650A (en) * | 2014-03-26 | 2014-07-02 | 中国科学院南海海洋研究所 | Breeding method of new thin-shell crassostrea hongkongensis variety |
| CN103947588A (en) * | 2014-04-16 | 2014-07-30 | 中国科学院南海海洋研究所 | Breeding method for Crassostrea sikamea by taking left shell radial ridge number as mark |
| CN104642228A (en) * | 2015-03-09 | 2015-05-27 | 中国科学院南海海洋研究所 | Breeding method for rapid growth strain of Hippocampus kelloggi |
| CN105494177A (en) * | 2015-08-24 | 2016-04-20 | 福建省水产研究所 | Seed production method for Portugal oyster quick-growing seed selection |
| CN105506162A (en) * | 2016-01-31 | 2016-04-20 | 中国海洋大学 | SNP marker related to rapid growth of pacific oysters and identification method as well as application thereof |
| CN106106287A (en) * | 2016-07-07 | 2016-11-16 | 苏州市相城区阳澄湖镇东枪塘水产养殖专业合作社 | One seed oyster artificial fecundation method |
| CN106413392A (en) * | 2014-04-18 | 2017-02-15 | 梅迪托公司 | Improved oyster farming method |
| CN106577415A (en) * | 2016-12-26 | 2017-04-26 | 中国海洋大学 | Seed production method of black-shell pacific oyster new strain |
| CN106614164A (en) * | 2016-12-26 | 2017-05-10 | 中国海洋大学 | Cultivation method for new strain of pure white shell pacific oyster |
| CN107841561A (en) * | 2016-09-18 | 2018-03-27 | 中国海洋大学 | The SNP marker and screening technique that long oyster shell form and aspect are closed |
| CN108012964A (en) * | 2017-12-26 | 2018-05-11 | 中国海洋大学 | A kind of breeding method of the long oyster new lines of cup type |
| CN108040938A (en) * | 2017-10-09 | 2018-05-18 | 中国科学院南海海洋研究所 | A kind of method that Hong Kong oyster triploid production performance is improved by Parent improvement |
| CN108157247A (en) * | 2018-01-23 | 2018-06-15 | 辽宁省海洋水产科学研究院 | A kind of breeding method of blood clam fast-growth strain |
| CN111183936A (en) * | 2020-02-11 | 2020-05-22 | 中国科学院南海海洋研究所 | Method for preparing novel strain of deep concave-shell type hongkong oyster by using virtual index polymerization method |
| CN115943913A (en) * | 2022-03-15 | 2023-04-11 | 中国海洋大学 | Breeding method for rapid-growth strain of crassostrea gigas |
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Cited By (28)
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|---|---|---|---|---|
| CN103719000A (en) * | 2013-12-20 | 2014-04-16 | 中国科学院南海海洋研究所 | Fertility recovery method for oyster distant hybrid sterility |
| CN103719000B (en) * | 2013-12-20 | 2015-09-02 | 中国科学院南海海洋研究所 | The fertility restorer method that one seed oyster distant hybrid is sterile |
| CN103798166A (en) * | 2014-01-24 | 2014-05-21 | 中国科学院南海海洋研究所 | Method for large-scale indoor artificial breeding of Hong Kong oysters in coastal region of South China |
| CN103814848A (en) * | 2014-02-28 | 2014-05-28 | 中国科学院南海海洋研究所 | Breeding method for new line of hybrid Crassostrea hongkongensis and Crassostrea gigas |
| CN103891650B (en) * | 2014-03-26 | 2015-07-15 | 中国科学院南海海洋研究所 | Breeding method of new thin-shell crassostrea hongkongensis variety |
| CN103891650A (en) * | 2014-03-26 | 2014-07-02 | 中国科学院南海海洋研究所 | Breeding method of new thin-shell crassostrea hongkongensis variety |
| CN103947588B (en) * | 2014-04-16 | 2015-09-02 | 中国科学院南海海洋研究所 | A kind of is the Kumamoto oyster producing method for seed marked with left shell radiation ridge number |
| CN103947588A (en) * | 2014-04-16 | 2014-07-30 | 中国科学院南海海洋研究所 | Breeding method for Crassostrea sikamea by taking left shell radial ridge number as mark |
| CN106413392A (en) * | 2014-04-18 | 2017-02-15 | 梅迪托公司 | Improved oyster farming method |
| CN106413392B (en) * | 2014-04-18 | 2020-08-21 | 梅迪托公司 | Improved oyster cultivation method |
| CN104642228A (en) * | 2015-03-09 | 2015-05-27 | 中国科学院南海海洋研究所 | Breeding method for rapid growth strain of Hippocampus kelloggi |
| CN105494177A (en) * | 2015-08-24 | 2016-04-20 | 福建省水产研究所 | Seed production method for Portugal oyster quick-growing seed selection |
| CN105494177B (en) * | 2015-08-24 | 2018-05-08 | 福建省水产研究所 | A kind of producing method for seed of the long breeding line of Portuguese oyster speed |
| CN105506162B (en) * | 2016-01-31 | 2020-05-05 | 中国海洋大学 | SNP (single nucleotide polymorphism) marker related to rapid growth of crassostrea gigas as well as identification method and application thereof |
| CN105506162A (en) * | 2016-01-31 | 2016-04-20 | 中国海洋大学 | SNP marker related to rapid growth of pacific oysters and identification method as well as application thereof |
| CN106106287A (en) * | 2016-07-07 | 2016-11-16 | 苏州市相城区阳澄湖镇东枪塘水产养殖专业合作社 | One seed oyster artificial fecundation method |
| CN107841561B (en) * | 2016-09-18 | 2021-04-16 | 中国海洋大学 | SNP (Single nucleotide polymorphism) marker related to oyster shell color and screening method |
| CN107841561A (en) * | 2016-09-18 | 2018-03-27 | 中国海洋大学 | The SNP marker and screening technique that long oyster shell form and aspect are closed |
| CN106614164A (en) * | 2016-12-26 | 2017-05-10 | 中国海洋大学 | Cultivation method for new strain of pure white shell pacific oyster |
| CN106577415B (en) * | 2016-12-26 | 2019-04-02 | 中国海洋大学 | A kind of producing method for seed of the long oyster new lines of black shell |
| CN106614164B (en) * | 2016-12-26 | 2019-04-30 | 中国海洋大学 | A kind of breeding method of the long oyster new lines of pure white shell |
| CN106577415A (en) * | 2016-12-26 | 2017-04-26 | 中国海洋大学 | Seed production method of black-shell pacific oyster new strain |
| CN108040938A (en) * | 2017-10-09 | 2018-05-18 | 中国科学院南海海洋研究所 | A kind of method that Hong Kong oyster triploid production performance is improved by Parent improvement |
| CN108012964A (en) * | 2017-12-26 | 2018-05-11 | 中国海洋大学 | A kind of breeding method of the long oyster new lines of cup type |
| CN108012964B (en) * | 2017-12-26 | 2020-09-01 | 中国海洋大学 | Method for cultivating new strain of cup-shaped crassostrea gigas |
| CN108157247A (en) * | 2018-01-23 | 2018-06-15 | 辽宁省海洋水产科学研究院 | A kind of breeding method of blood clam fast-growth strain |
| CN111183936A (en) * | 2020-02-11 | 2020-05-22 | 中国科学院南海海洋研究所 | Method for preparing novel strain of deep concave-shell type hongkong oyster by using virtual index polymerization method |
| CN115943913A (en) * | 2022-03-15 | 2023-04-11 | 中国海洋大学 | Breeding method for rapid-growth strain of crassostrea gigas |
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