CN103877568A - Compound coneflower preparation preparation method - Google Patents
Compound coneflower preparation preparation method Download PDFInfo
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- CN103877568A CN103877568A CN201210565086.3A CN201210565086A CN103877568A CN 103877568 A CN103877568 A CN 103877568A CN 201210565086 A CN201210565086 A CN 201210565086A CN 103877568 A CN103877568 A CN 103877568A
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- echinacea
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Abstract
The invention provides a compound coneflower preparation, wherein a use ratio of effective components such as a coneflower extract and lysozyme is 3:1, the total content of the coneflower extract and the lysozyme is 25%, and contents of auxiliary materials such as sucrose and dextrin are respectively 60% and 15%. According to the present invention, the selected coneflower extract is obtained by extracting a coneflower herb with an alkaline ethanol solution and lysozyme; the polysaccharide content exceeds 20%, the polyphenol content exceeds 8%, and the chicoric acid content exceeds 5%; and the composite preparation has functions of animal immunity improving, anti-bacterial and anti-virus, and can be used for preparation of drugs for prevention and treatment of animal diseases, especially prevention and treatment of chicken diseases caused by escherichia coli infection.
Description
Technical field
The invention belongs to biological product formulation art, relate to a kind of animal immune protective agent, relate to a kind of preparation method and application of compound recipe Echinacea preparation.
Background technology
Echinacea is a kind of Echinacea plant that originates in North America and Canada south, and China introduces a fine variety some areas.In Echinacea, contain chicoric acid, polysaccharide, polyphenol, polyacetylene and alkaloid.There is the immunity of raising and antibacterial and anti-inflammation functions.Polysaccharide can excite the cell of health to go infection; Polyphenols can improve the immunity of body, and their energy stimulating immune system cellular activities, make virus or antibacterial death.Especially chicoric acid is one of immune active ingredient very important in Echinacea.Pharmacological research in recent years shows, chicoric acid has the immunologic function of enhancing and antiinflammatory action, and can suppress hyaluronidase, and protection collagen protein is avoided the impact of the free radical that can cause degraded.Domestic clinical trial also confirms that the diseases such as the infectious bursal disease of Echinacea to chicken, infectious bronchitis, atypical newcastle have good curative effect.
But, only depend on the immune system that stimulates body, improve the immunity of body, a little less than its bactericidal antiphlogistic effect.Curative effect is unsatisfactory.Therefore, the medicine of the other types that searching can have complementary functions with Echinacea, is another outlet of further developing Echinacea purposes.
Lysozyme is a kind of alkaline enzyme that can be hydrolyzed mucopolysaccharide in pathogenic bacterium, has the natural infection material of bactericidal action.The effects such as that this enzyme has is antibacterial, antiinflammatory, antiviral.The antimicrobial spectrum of lysozyme is wider, is not only confined to gram positive bacteria, and part gram negative bacteria is also had to inhibition.Lysozyme directly kill pathogenic bacterium function, can make up the deficiency of Echinacea.But lysozyme itself is without the effect of enhancing immunity, and Echinacea raising immunity of organisms function is extremely strong, can excite the cell of health to go infection, can make up the deficiency of lysozyme.
Still less to the compound research of Echinacea and lysozyme both at home and abroad, mostly both are independent use.So the complex of developing both has broad prospects.
Summary of the invention
The object of this invention is to provide a kind of compound recipe Echinacea preparation, it is taking Echinacea extract and lysozyme as effective ingredient, processes taking sucrose and dextrin as adjuvant.
The inventor is definite through the drug effect contrast screening of bacteriostatic test contrast and practical application, and in the compound preparation of above-mentioned Echinacea extract and lysozyme, the quality usage ratio of effective ingredient Echinacea extract and lysozyme is 3:1.
The compound preparation of Echinacea extract of the present invention and lysozyme is a kind of compound granular formulation, and wherein, total consumption of effective ingredient Echinacea extract and lysozyme is 24%, total consumption 76% of adjuvant sucrose and dextrin, wherein, dosage of sucrose is 65%, and dextrin consumption is 11%.
The compound preparation of Echinacea extract provided by the present invention and lysozyme, that Echinacea extract and the lysozyme of studying preparation by the inventor is effective ingredient, add that suitable auxiliary element is composite to be processed into, in the Echinacea extract using, polyoses content exceedes 20%, polyphenol content exceedes 8%, and chicoric acid content exceedes 5%; The Ministry of Public Health ministry standard standard that the lysozyme using is carried out, content is 6350 units/mg.
Above-mentioned compound recipe Echinacea preparation has raising animal immunizing power, antibacterial, antiviral functions, can be used as prevention and treatment Animal diseases medicine.
Because the extracting method of Echinacea extract is in the market mainly simple water logging and alcohol extraction, cause extraction efficiency on the low side, exist a certain active constituent content higher, and the lower shortcoming of all the other active constituent contents, thereby affect result of use.For ensureing its curative effect, inventor research provides a kind of extracting method of uniqueness, and the content that makes polysaccharide, polyphenol and chicoric acid in prepared extract is all apparently higher than the prepared Echinacea extract of conventional method in the market.
Echinacea extract provided by the present invention adopts alkaline ethanol solution and lysozyme that Echinacea medical material is extracted and obtained, and wherein polyoses content exceedes 20%, and polyphenol content exceedes 8%, and chicoric acid content exceedes 5%.
The preparation method of Echinacea extract provided by the present invention is that in 20～70% alcoholic solution that are 12 by Echinacea medical material at pH, reflux, extract, is obtained.
Method.
detailed description of the invention
In the present invention, the preparation method step of Echinacea extract and lysozyme compound preparation is as follows:
1, get Echinacea extract and mix with lysozyme, add the distilled water of 1.5 times of mixture qualities, stir, obtain Echinacea extract-lysozyme mixed liquor;
2, by dextrin and sucrose, mix homogeneously, drops into Echinacea extract-lysozyme mixed liquor, then under 50 DEG C of conditions, mixture is dried, and obtains effective ingredient and be 25% compound recipe Echinacea granular preparation.
3 or get dextrin and sucrose, mix homogeneously, drops into fluid bed, then Echinacea is got to thing-lysozyme mixed liquor under 50 DEG C of conditions and is sprayed onto on the mixture of dextrin and sucrose, obtains effective ingredient and be 20% compound recipe Echinacea granular preparation.
Further illustrate the present invention below in conjunction with instantiation.
embodiment 1
Get Echinacea extract 192g, lysozyme 48g, adds purified water 290g, stirs, and obtains Echinacea extract-lysozyme mixed liquor; Get again dextrin 160g, sucrose 600g, mix with Echinacea extract-lysozyme mixed liquor; Then under 50 DEG C of conditions, mixture is dried, and obtains effective ingredient and be 24% Echinacea lysozyme compound granular formulation; Wherein, Echinacea extract 19.2%, lysozyme 4.8%, sucrose 60%, dextrin 16%.
embodiment 2
Get Echinacea extract 180g, lysozyme 60g, adds purified water 290g, stirs, and obtains Echinacea extract-lysozyme mixed liquor; Get dextrin 150g, sucrose 610g, mix homogeneously, drops into fluid bed again; Then Echinacea is got to thing-lysozyme mixed liquor under 50 DEG C of conditions and be sprayed onto on the mixture of dextrin and sucrose, obtain effective ingredient and be Echinacea-lysozyme compound granular formulation of 24%; Wherein, Echinacea extract 18%, lysozyme 6%, sucrose 61%, dextrin 15%.
embodiment 3
Get Echinacea extract 160g, lysozyme 80g, adds purified water 290g, stirs, and obtains Echinacea extract-lysozyme mixed liquor; Get again dextrin 160g, sucrose 600g, mix with Echinacea extract-lysozyme mixed liquor; Then under 50 DEG C of conditions, mixture is dried, and obtains effective ingredient and be 24% Echinacea lysozyme compound granular formulation. wherein, and Echinacea extract 10%, lysozyme 10%, sucrose 64%, dextrin 16%.
Claims (8)
1. a compound recipe Echinacea preparation, the quality usage ratio that it is characterized in that effective ingredient Echinacea extract and lysozyme is (2 ~ 4): 1.
2. the compound recipe Echinacea preparation as described in claim 1, it is characterized in that by 24% effective ingredient and adjuvant sucrose and sticks with paste accurately machined a kind of 25% compound granular formulation.
3. compound recipe Echinacea preparation as claimed in claim 2, it is characterized in that in said compound granular formulation, the usage ratio of Echinacea extract and lysozyme is (2 ~ 4): 1, and the mass fraction of each component is Echinacea extract 18%, lysozyme 6%, sucrose 65%, dextrin 11%.
4. the compound recipe Echinacea preparation as described in claim 1-3, is characterized in that used Echinacea extract is to adopt alkaline ethanol solution and lysozyme to carry out extracted twice acquisition to Echinacea medical material; Wherein: polyoses content exceedes 20%, polyphenol content exceedes 8%, and chicoric acid content exceedes 5%.
5. the preparation method of compound recipe Echinacea preparation as claimed in claim 4, is characterized in that adopting first 20～70% alcoholic solution 0.1mol/L sodium hydroxide solution being adjusted into 12 by pH, by Echinacea medical material reflux, extract, in alkaline ethanol solution.
6. the preparation method of compound recipe Echinacea preparation as claimed in claim 5, is characterized in that concrete preparation method is as follows:
Get 8 parts of concentration and be 20% to 70% alcoholic solution, regulate pH value to 12 with NaOH; By 1 part of Echinacea pulverizing medicinal materials, be soaked in above-mentioned alkaline ethanol solution, reflux, extract, 3 times after some hours, each extraction time, more than 2 hours, filters, and merging filtrate obtains Echinacea extracting solution; Filtrate is regulated to pH to 4 ~ 6 with HCl, leave standstill 3 hours, filter, precipitation drying and crushing, obtains Echinacea precipitated powder; Filtrate recycling ethanol is extremely without alcohol taste.
7. concentrated spray mixes gained Echinacea powder after being dried with Echinacea precipitated powder, obtains Echinacea extract.
8. the Echinacea extract as described in claim 4-5 and the compound granular formulation of lysozyme, it is characterized in that it has raising animal immunizing power, anti-stress, sterilization, antiviral functions, can be used as prevention and treatment Animal diseases and particularly prevent and treat the medicine because of the fowl disease of coli-infection initiation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210565086.3A CN103877568A (en) | 2012-12-24 | 2012-12-24 | Compound coneflower preparation preparation method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210565086.3A CN103877568A (en) | 2012-12-24 | 2012-12-24 | Compound coneflower preparation preparation method |
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| CN103877568A true CN103877568A (en) | 2014-06-25 |
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| CN201210565086.3A Pending CN103877568A (en) | 2012-12-24 | 2012-12-24 | Compound coneflower preparation preparation method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104127462A (en) * | 2014-07-01 | 2014-11-05 | 青岛蔚蓝生物股份有限公司 | Injection-grade echinacea extractive and injection thereof |
| CN104257714A (en) * | 2014-09-11 | 2015-01-07 | 华南农业大学 | Echinacea purpurea (L.) Moench root granules as well as preparation method and application thereof to preparation of swine and chicken vaccine immunopotentiators |
-
2012
- 2012-12-24 CN CN201210565086.3A patent/CN103877568A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104127462A (en) * | 2014-07-01 | 2014-11-05 | 青岛蔚蓝生物股份有限公司 | Injection-grade echinacea extractive and injection thereof |
| CN104127462B (en) * | 2014-07-01 | 2017-02-01 | 青岛蔚蓝生物股份有限公司 | Injection-grade echinacea extractive and injection thereof |
| CN104257714A (en) * | 2014-09-11 | 2015-01-07 | 华南农业大学 | Echinacea purpurea (L.) Moench root granules as well as preparation method and application thereof to preparation of swine and chicken vaccine immunopotentiators |
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Application publication date: 20140625 |