CN103877155A - Chinese herbal preparation for treating trichodiniasis of fishes - Google Patents
Chinese herbal preparation for treating trichodiniasis of fishes Download PDFInfo
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- CN103877155A CN103877155A CN201410137571.XA CN201410137571A CN103877155A CN 103877155 A CN103877155 A CN 103877155A CN 201410137571 A CN201410137571 A CN 201410137571A CN 103877155 A CN103877155 A CN 103877155A
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- chinese herbal
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Abstract
The invention relates to a Chinese herbal preparation for treating trichodiniasis of fishes. The Chinese herbal preparation is characterized by consisting of the following materials in percentage by weight: 5-25% of a radix sophorae flavescentis aqueous extract, 5-25% of a melia azedarach aqueous extract, 5-25% of a surfactant, 10-20% of a dissolving agent and the balance of a solvent, wherein the surfactant is Tween-20; the dissolving agent is dimethyl sulfoxide; the solvent is water. The Chinese herbal preparation disclosed by the invention overcomes the shortcomings of common basic medicines, and is simple and convenient to apply and is rapid and uniform to spread after being sprayed in a whole process; the Chinese herbal preparation is used for treating trichodiniasis of fishes and is applicable to rainy day as well. The Chinese herbal preparation is free from drug tolerance and drug resistance, and is free from toxin and residue as well as pollution to water quality.
Description
Technical field
The present invention relates to a kind of Chinese herbal and crude drugs preparations for the treatment of aquatic animal trichodinasis, particularly a kind of Chinese herbal and crude drugs preparations for the treatment of Fish trichodinasis.
Background technology
Trichodinasis is to be colonized on the body surfaces of Fish and the gill and caused by Trichodina, and the area of respectively breeding fish in the whole nation has popular, all has throughout the year generation, can endanger various fresh-water fishes, marine fish, and main harm fry, fingerling can cause large quantities of death when serious.Between annual 5～August, fry, fingerling are often therefore sick and large quantities of death causes and has a strong impact on aquaculture.
Prevent and treat at present trichodinasis and still rely on special pesticide medicine, the basic medicine for the treatment of this disease is a lot, mainly contains copper sulfate, mixture of ferrous sulfate, ambam, Mercury protonitrate., malachite green oxalate and formalin etc.But what life-time service brought is the series of problems such as environmental pollution, drug residue, drug resistance, also become the Green Trade Barrier problem of present food security and product water outlet.This Chinese herbal and crude drugs preparations is the medicine from extracted form natural plant, there is low toxicity, low-residual, poison lower to non-target organism, the advantages such as environmentally safe, " the medicine public hazards " that can reduce or avoid current chemicals to bring, are development trend and a large focus of Aquatic product disease control.
Summary of the invention
The object of the present invention is to provide a kind of Chinese herbal and crude drugs preparations for the treatment of Fish trichodinasis.The present invention also aims to provide a kind of preparation method of Chinese herbal and crude drugs preparations for the treatment of Fish trichodinasis.Object of the present invention is further a kind of application of Chinese herbal and crude drugs preparations for the treatment of Fish trichodinasis.
Technical scheme of the present invention is: a kind of Chinese herbal and crude drugs preparations for the treatment of Fish trichodinasis, it is characterized in that being formed by the following material of weight percentage: Radix Sophorae Flavescentis water extract 5%-25%, Melia azedarach L. water extract 5%-25%, surfactant 5%-25%, lytic agent 10%-20%, surplus is solvent; Described surfactant is tween 20, and described lytic agent is dimethyl sulfoxide, and described solvent is water.
Described a kind of Chinese herbal and crude drugs preparations for the treatment of Fish trichodinasis, is characterized in that the weight percentage of described Radix Sophorae Flavescentis water extract is 5%-25%, is preferably 15%-20%.
Described a kind of Chinese herbal and crude drugs preparations for the treatment of Fish trichodinasis, is characterized in that the weight percentage of described Melia azedarach L. water extract is 5%-25%, is preferably 15%-20%.
Described a kind of Chinese herbal and crude drugs preparations for the treatment of Fish trichodinasis, the weight percentage that it is characterized in that described surfactant is 6%-8%.
The technical scheme of preparation method of the present invention is:
Treat a preparation method for Fish Trichodina Chinese herbal and crude drugs preparations, it is characterized in that comprising the following steps:
1) take a certain amount of Radix Sophorae Flavescentis and add the water of 5 times of volumes, at 60-70 DEG C, extracting 4 hours, become extractum and save backup by filtering drying under reduced pressure;
2) take a certain amount of Melia azedarach L. and add the water of 5 times of volumes, at 60-70 DEG C, extracting 4 hours, become extractum and save backup by filtering drying under reduced pressure;
3) according to above-mentioned substance ratio, take Radix Sophorae Flavescentis water extract, Melia azedarach L. water extract, surfactant, lytic agent, solvent, for subsequent use;
4) Radix Sophorae Flavescentis water extract, Melia azedarach L. water extract and lytic agent are mixed to completely and dissolve;
5) surfactant, solvent are added in the mixed solution of step 4) gained and stir, must treat the Chinese herbal and crude drugs preparations of Fish trichodinasis.
The application of Chinese herbal and crude drugs preparations of the present invention, is characterized in that: the Chinese herbal and crude drugs preparations of preparing by the present invention is directly splashed in pond, and using dosage is 0.75g-1.5g/m
3.
The Chinese herbal and crude drugs preparations of above-mentioned treatment Fish trichodinasis is in the application of killing in Fish trichodinasis, and insect killing effect is good, and also has low toxicity, low-residual, lower to non-target organism murder by poisoning, and the advantages such as environmentally safe are safe and reliable.
The present invention is that prescription is carried out in the pharmacological action based on following medicine:
Radix Sophorae Flavescentis: parasite killing goes to amass; Diuretic;
Melia azedarach L.: parasite killing; Clearing heat and expelling damp.
Detailed description of the invention
Now, in conjunction with embodiments of the invention, the present invention is described in detail, so that advantage of the present invention is more obvious, and by test, beneficial effect of the present invention is described.
Embodiment 1
Take Radix Sophorae Flavescentis and the each 5kg of Melia azedarach L. water extract, stirring and dissolving in 10 kg dimethyl sulfoxide, takes 5 kg surfactant tween 20s, aqueous solvent 75 kg, mixing and stirring and get final product.
Embodiment 2
Take each 10 kg of Radix Sophorae Flavescentis and Melia azedarach L. water extract, stirring and dissolving in 20 kg dimethyl sulfoxide, takes 10 kg surfactant tween 20s, aqueous solvent 50 kg, mixing and stirring and get final product.
Embodiment 3
Take each 15 kg of Radix Sophorae Flavescentis and Melia azedarach L. water extract, stirring and dissolving in 20 kg dimethyl sulfoxide, takes 10 kg surfactant tween 20s, aqueous solvent 40 kg, mixing and stirring and get final product.
Embodiment 4
Take each 20 kg of Radix Sophorae Flavescentis and Melia azedarach L. water extract, stirring and dissolving in 20 kg dimethyl sulfoxide, takes 10 kg surfactant tween 20s, aqueous solvent 30 kg, mixing and stirring and get final product.
Embodiment 5
Take Radix Sophorae Flavescentis and Melia azedarach L. water extract 25 kg, stirring and dissolving in 25 kg dimethyl sulfoxide, takes 10 kg surfactant tween 20s, aqueous solvent 15 kg, mixing and stirring and get final product.
Above-described embodiment can be enumerated a lot, does not enumerate at this, selects as long as the parameter area according to technical scheme of the present invention, can prepare the Chinese herbal and crude drugs preparations that reaches beneficial effect of the present invention.Said herbal medicine preparation can directly be splashed in pond, and using dosage is: 0.75g-1.5g/m
3.
Below by test, control and the killing effect of said herbal medicine preparation to Fish trichodinasis is described.
Experiment 1
(1) experimental condition
No. 1 fishpond of A, 11.2 mu of aperture areas, 1.5 meters of the depth of waters, soil property is loam, south-north direction, east is water channel, fishpond is adjoined in south, north, west.No. 2 fishponds, 10.7 mu of aperture areas, 1.5 meters of the depth of waters, soil property is loam, south-north direction, west is water channel, fishpond is adjoined in south, north, the east.
B breeding water
Water source: Dongting Lake lake water, Ph value 7.2, transparency 30cm, water temperature 18-26 degree.
C test fish products kind
No. 1 fishpond be local produce two age Ctenopharyngodon idellus 2240 tails, average specification is 350g, separately joins the Aristichthys nobilis, silver carp, Carassius auratus of some etc.No. 2 fishponds be local produce two age Ctenopharyngodon idellus 2100 tails, average specification is 360g, separately joins the Aristichthys nobilis, silver carp, Carassius auratus of some etc.
D disease
No. 1 the sick fish in fishpond shows as fish body blackout, the solely trip that peels off, and dead 51 tails before test, the sick fish of microscopy, has 8 of Trichodinas on the fish gill in an average visual field.No. 2 the sick fish in fishpond shows as fish body blackout, the solely trip that peels off, and dead 37 tails before test, the sick fish of microscopy, has 5 of Trichodinas on the fish gill in an average visual field.
E test method
No. 1 fishpond Chinese herbal and crude drugs preparations 8.4kg of the present invention for first day, full pool spilling head, dead fish 13 tails, the sick fish of microscopy, has 4 of Trichodinas on the fish gill in an average visual field, and trichodinasis symptom is improved, second day with Chinese herbal and crude drugs preparations 8.4kg of the present invention, full pool spilling head, dead fish 7 tails, the sick fish of microscopy, has 1 of Trichodina on the fish gill in an average visual field, and the 3rd day with Chinese herbal and crude drugs preparations 8.4kg of the present invention, full pool spilling head, fish swimming freely, is quick on the draw, without dead fish, microscopy is not found Trichodina.No. 2 not medications of fishpond, dead fish 11 tails of first day, the sick fish of microscopy, on the fish gill in an average visual field, there are 5 of Trichodinas, second day dead fish 20 tail, the sick fish of microscopy, on the fish gill in an average visual field, there are 6 of Trichodinas, the 3rd day dead fish 27 tails, the sick fish of microscopy, has 8 of Trichodinas on the fish gill in an average visual field.
(2) result of the test
No. 1 dead 14 tails of fishpond fish, cure rate is 99.1%, No. 2 not medication of fishpond, fish death is on the rise.
Experiment 2
(1) experimental condition
No. 1 fishpond of A, 4.5 mu of aperture areas, 1.5 meters of the depth of waters, soil property is loam, east-west, and east is road, and north is water channel, and fishpond is adjoined in south, west.No. 2 fishponds, 4.8 mu of aperture areas, 1.6 meters of the depth of waters, soil property is loam, south-north direction, west is water channel, and south is road, and fishpond is adjoined on east, north.
B breeding water
Water source: Yangtze River Water, Ph value 7.1, transparency 30cm, water temperature 18-28 degree.
C test fish products kind
No. 1 fishpond is local Ctenopharyngodon idellus Seedling 1800 tails of producing, and average specification is 150g.No. 2 fishponds are local Ctenopharyngodon idellus 2000 tails of producing, and average specification is 152g.
D disease
No. 1 the sick fish in fishpond shows as fish body blackout, and solely trip peels off.Have again in groups around the mad trip of pool side.Dead 21 tails before test, the sick fish of microscopy, has 5 of Trichodinas on the fish gill in an average visual field.No. 2 the sick fish in fishpond shows as fish body blackout, and solely trip peels off.Have again in groups around the mad trip of pool side.Dead 32 tails before test, the sick fish of microscopy, has 6 of Trichodinas on the fish gill in an average visual field.
E test method
No. 1 fishpond Chinese herbal and crude drugs preparations 3.4kg of the present invention for first day, full pool spilling head, dead fish 17 tails, the sick fish of microscopy, has 2 of Trichodinas on the fish gill in an average visual field, and trichodinasis symptom is improved, second day with Chinese herbal and crude drugs preparations 3.4kg of the present invention, full pool spilling head, dead fish 5 tails, the sick fish of microscopy, has 1 of Trichodina on the fish gill in an average visual field, and the 3rd day with Chinese herbal and crude drugs preparations 3.4kg of the present invention, full pool spilling head, fish swimming freely, is quick on the draw, without dead fish, microscopy is not found Trichodina.No. 2 not medications of fishpond, dead fish 23 tails of first day, the sick fish of microscopy, on the fish gill in an average visual field, there are 6 of Trichodinas, second day dead fish 37 tail, the sick fish of microscopy, on the fish gill in an average visual field, there are 6 of Trichodinas, the 3rd day dead fish 40 tails, the sick fish of microscopy, has 8 of Trichodinas on the fish gill in an average visual field.
(3) result of the test
No. 1 dead 22 tails of fishpond fish, cure rate is 98.8%, No. 2 not medication of fishpond, fish death is on the rise.
Claims (6)
1. treat the Chinese herbal and crude drugs preparations of Fish trichodinasis for one kind, it is characterized in that being formed by the following material of weight percentage: Radix Sophorae Flavescentis water extract 5%-25%, Melia azedarach L. water extract 5%-25%, surfactant 5%-25%, lytic agent 10%-20%, surplus is solvent; Described surfactant is tween 20, and described lytic agent is dimethyl sulfoxide, and described solvent is water.
2. the Chinese herbal and crude drugs preparations for the treatment of Fish trichodinasis according to claim 1, is characterized in that the weight percentage of described Radix Sophorae Flavescentis water extract is 5%-25%, is preferably 15%-20%.
3. treatment Fish Trichodina Chinese herbal and crude drugs preparations according to claim 1, is characterized in that the weight percentage of described Melia azedarach L. water extract is 5%-25%, is preferably 15%-20%.
4. the Chinese herbal and crude drugs preparations for the treatment of Fish trichodinasis according to claim 1, is characterized in that the weight percentage of described surfactant is preferably 6%-8%.
5. the preparation method of the Chinese herbal and crude drugs preparations for the treatment of Fish trichodinasis as claimed in claim 1, is characterized in that comprising the following steps:
1) take a certain amount of Radix Sophorae Flavescentis and add the water of 5 times of volumes, at 60-70 DEG C, extracting 4 hours, become extractum and save backup by filtering drying under reduced pressure;
2) take a certain amount of Melia azedarach L. and add the water of 5 times of volumes, at 60-70 DEG C, extracting 4 hours, become extractum and save backup by filtering drying under reduced pressure;
3) according to above-mentioned substance ratio, take Radix Sophorae Flavescentis water extract, Melia azedarach L. water extract, surfactant, lytic agent, solvent, for subsequent use;
4) Radix Sophorae Flavescentis water extract, Melia azedarach L. water extract and lytic agent are mixed to completely and dissolve;
5) surfactant, solvent are added in the mixed solution of step 4) gained and stir, must treat Fish Trichodina Chinese herbal and crude drugs preparations.
6. the application of the Chinese herbal and crude drugs preparations for the treatment of Fish trichodinasis as claimed in claim 1, is characterized in that: the Chinese herbal and crude drugs preparations of preparing by the present invention is directly splashed in pond, and using dosage is 0.75g-1.5g/m
3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410137571.XA CN103877155A (en) | 2014-04-08 | 2014-04-08 | Chinese herbal preparation for treating trichodiniasis of fishes |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410137571.XA CN103877155A (en) | 2014-04-08 | 2014-04-08 | Chinese herbal preparation for treating trichodiniasis of fishes |
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| CN103877155A true CN103877155A (en) | 2014-06-25 |
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| CN201410137571.XA Pending CN103877155A (en) | 2014-04-08 | 2014-04-08 | Chinese herbal preparation for treating trichodiniasis of fishes |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105878838A (en) * | 2016-06-03 | 2016-08-24 | 李良平 | Composition for controlling fry trichodiniasis |
| CN107088221A (en) * | 2017-03-29 | 2017-08-25 | 华南农业大学 | A kind of Chinese herbal medicine for preventing and treating fish trichodinasis, pharmaceutical chemistry and preparation method thereof |
| CN107744550A (en) * | 2017-10-29 | 2018-03-02 | 南宁学院 | A kind of composition for preventing and treating mandarin sturgeon trichodinasis and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101961399A (en) * | 2010-06-23 | 2011-02-02 | 天津生机集团股份有限公司 | Preparation method of insecticide for aquatic animals |
-
2014
- 2014-04-08 CN CN201410137571.XA patent/CN103877155A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101961399A (en) * | 2010-06-23 | 2011-02-02 | 天津生机集团股份有限公司 | Preparation method of insecticide for aquatic animals |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105878838A (en) * | 2016-06-03 | 2016-08-24 | 李良平 | Composition for controlling fry trichodiniasis |
| CN107088221A (en) * | 2017-03-29 | 2017-08-25 | 华南农业大学 | A kind of Chinese herbal medicine for preventing and treating fish trichodinasis, pharmaceutical chemistry and preparation method thereof |
| CN107744550A (en) * | 2017-10-29 | 2018-03-02 | 南宁学院 | A kind of composition for preventing and treating mandarin sturgeon trichodinasis and preparation method thereof |
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Application publication date: 20140625 |