CN103861078A - Snake-scorpion pain-relieving and wind-dispelling liquor and preparation method thereof - Google Patents
Snake-scorpion pain-relieving and wind-dispelling liquor and preparation method thereof Download PDFInfo
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Abstract
The invention relates to the technical field of medicinal liquor, and in particular relates to snake-scorpion pain-relieving and wind-dispelling liquor and a preparation method thereof. The liquor comprises medicinal cyathula root, angelica pubescens, cortex acanthopanacis, cortex erythrinae, cinnamon, ligusticum wallichii, Chinese angelica, radix codonopsis, radix clematidis, divaricate saposhnikovia root, eucommia, monkshood, tripterygium glycosides, prepred nux vomica, liquorice, snake venom polypeptide, scorpion venom polypeptide, honey and distilled spirit. The medicinal liquor is prepared by adopting an infiltration method, the preparation method is simple, the liquor is convenient to take and tasted comfortably, and especially can be used for effectively treating rheumatism arthralgia, tendon spasm and other diseases.
Description
Technical field
The present invention relates to medicated wine technical field, particularly relate to a kind of snakes and scorpions analgesia liquor for treating rheumatism and preparation method thereof.
Background technology
Wine have the title of " length of hundred medicines ".Modern study shows, the main component ethanol (being commonly called as ethanol) of wine is a kind of good semi-polarity organic solvent.The Multiple components of Chinese medicine is as all more soluble in ethanol in alkaloid, salt, tannin, volatile oil, organic acid, resin, saccharide and partial pigment (as chlorophyll, phylloxanthin) etc.Ethanol not only has good penetrance, is easy to enter in medical material histiocyte, and performance dissolution, promotes displacement, diffusion, is conducive to improve leaching velocity and leaching effect; Also have antisepsis, can delay the hydrolysis of many medicines, strengthen the stability of medicament.
Using the medicated wine being integrated as the wine of beverage and the medicine promoting health and curing diseases " molten ", not only there is preparation, take easyly, the property of medicine is stable, and advantage safely and effectively, more because wine assisting drug, wine help medicine gesture and give full play to effect, raising curative effect.
Just on the books in the Shennong's Herbal that Serpentis class is used as medicine before two thousand years, and whole body has medical value.Carnis Serpentis has the effect of the wind dispelling of invigorating blood circulation, expectorant damp eliminating, invigorating the spleen and replenishing QI, and the diseases such as rheumatic arthritis, numb limbs and tense tendons, Qi and blood deficiency, infantile convulsion epilepsy and skin pruritus are had to good curative effect.Fel Serpentis, snake bone, Periostracum Serpentis (a prescription name Exocarpium Juglandis Immaturum, is commonly called as Serpentis shell, Serpentis clothing), to treatment sciatica, migraine, rheumatoid arthrosis pain and terminal cancer, leprosy etc. have good curative effect.Wherein also expensive ten times of snake venom cost ratio gold are medicine sources very in short supply on international market.
Scorpio laudatory title mountain shrimp, Scorpio, the medical science medicinal history of existing several thousand, records Scorpio and has the effect of " endogenous wind stopping spasmolytic, dispersing pathogen accumulation, removing obstruction in the collateral to relieve pain " in detail according to Compendium of Material Medica.The diseases such as facial hemiparalysis, rheumatism, scapulohumeral periarthritis are had to significant curative effect, and have anti-cancer, anticancer effect.Scorpion wine, the analgesia of Chang Yinke endogenous wind stopping, detoxicating and resolving stagnation of pathogens, removing obstruction in the collateral to relieve pain..22 seed amino acids that scorpion wine contains needed by human body and various trace elements and vitamin, taurine etc.; without any side effects to patient; it is sutable for men, women, and children and high-grade nutrient agriculture tonic building body; its function can two-way raising function of human body, physiological function; nervous system, cardio-cerebrovascular regulating liver-QI, kidney, skin are had to good curative effect, treatment rheumatism, shoulder pain, shoulder inflammation, headache, facial paralysis, women's lumbago etc. are had to positive effect.
But existing snakes and scorpions wine is all generally that whole piece Serpentis and Scorpio are arranged in pairs or groups after some Chinese medicines, is directly put in Chinese liquor and soaks, and directly drinks use when treatment.Drink on the one hand because there being medical material impurity inconvenient, its therapeutic efficiency is clear and definite not, on the other hand because snakes and scorpions is that whole piece is put into, makes to dissolve in wine multiple material.And snakes and scorpions has certain toxicity, it is steep in wine and can not disorderly drink: the toxicity of the poisonous snakes such as Naja, Trimeresurus stejnegeri is all very large, and can not take orally easily with the medicated wine of its bubble can not external.Snake venom permeable enter skin, affect renal function, also the unknown toxicity entail dangers to life in some Serpentis body.
Summary of the invention
In order to address the above problem, the object of the invention is to, provide a kind of safe, drink the liquor for treating rheumatism of snakes and scorpions analgesia easily; It not only effectively treats the disease such as rheumatic arthralgia, muscles and bones contracture, and manufacture method is simple, evident in efficacy.
Two of object of the present invention is to provide the preparation method of described snakes and scorpions analgesia liquor for treating rheumatism.
The present invention is achieved through the following technical solutions:
A kind of snakes and scorpions analgesia liquor for treating rheumatism, is mainly made up of former medicament extract and wine; Solid night between described former medicine and wine is than being 1:12～1:13(grams per milliliter);
Described former medicated bag is drawn together the following material according to listed as parts by weight:
5～15 parts of Radix Cyathulaes, 5～15 parts of Radix Angelicae Pubescentiss, 5～15 parts of Cortex Acanthopanciss, 5～15 parts of Cortex erythrinaes, 5～15 parts of Cortex Cinnamomis, 5～15 parts of Rhizoma Chuanxiongs, 5～10 parts of Radix Angelicae Sinensis, 5～15 parts of Radix Codonopsis, 5～10 parts of Radix Clematidis, 5～10 parts of Radix Saposhnikoviaes, 5～10 parts of the Cortexs Eucommiae, 5～10 parts of Radix Aconitis, 1～3 part of Radix Tripterygii Wilfordii, 1～3 part of Semen Strychni (processed), 15～25 parts, Radix Glycyrrhizae, 0.1～0.5 part of N-GEECDCGSPENPCCD, 0.1～0.3 part of Buthotoxin polypeptide.
Preferably, described former medicated bag is drawn together the following material according to listed as parts by weight:
0.15 part of 10 parts of Radix Cyathulaes, 10 parts of Radix Angelicae Pubescentiss, 10 parts of Cortex Acanthopanciss, 10 parts of Cortex erythrinaes, 10 parts of Cortex Cinnamomis, 10 parts of Rhizoma Chuanxiongs, 10 parts of Radix Angelicae Sinensis, 10 parts of Radix Codonopsis, 5 parts of Radix Clematidis, 5 parts of Radix Saposhnikoviaes, 5 parts of the Cortexs Eucommiae, 5 parts of Radix Aconitis, 3 parts of Radix Tripterygii Wilfordiis, 1.5 parts of Semen Strychni (processed)s, 20 parts, Radix Glycyrrhizae, 0.30 part of N-GEECDCGSPENPCCD and Buthotoxin polypeptide.
Preferably, described liquid substance also comprises Mel, and the volume ratio of described Mel and Chinese liquor is 1:30.
Preferably, described Radix Tripterygii Wilfordii band dermatome.
Preferably, described Semen Strychni (processed) is processed gained for sand scalds big gun.
Preferably, described wine is the Chinese liquor of 55 ± 3 °.
The preparation method of described snakes and scorpions analgesia liquor for treating rheumatism, is specially:
First the Radix Cyathulae of described amount, Radix Angelicae Pubescentis, Cortex Acanthopancis, Cortex erythrinae, Cortex Cinnamomi, Rhizoma Chuanxiong, Radix Angelicae Sinensis, Radix Codonopsis, Radix Clematidis, Radix Saposhnikoviae, the Cortex Eucommiae, Radix Aconiti, Radix Tripterygii Wilfordii, Semen Strychni (processed), Radix Glycyrrhizae 15 taste traditional Chinese medicine powders are broken into coarser powder, adopt percolation to make former drug filtrate;
In former drug filtrate, add the Mel of described amount to stir evenly again, after the standing a few days, leach medicinal liquid;
Finally add N-GEECDCGSPENPCCD and the Buthotoxin polypeptide of described amount, after stirring evenly, make described snakes and scorpions analgesia liquor for treating rheumatism.
Wherein, the preparation of described former drug filtrate:
First described 15 taste traditional Chinese medicine powders are broken into coarse powder, add appropriate Chinese liquor to infiltrate 2～4 hours, make medicinal material coarse powder fill a part expansion;
Then, gradation packs equably bottom into and is lined with in the percolation filter (percolation filtration extraction pot) of absorbent cotton, installs to compress with wooden stick afterwards at every turn; Install Chinese crude drug, gauze in upper cover, and press the clean handstone of one deck, in order to avoid medicated powder is floated after adding Chinese liquor.
Then open the switch of percolation filter end opening, add Chinese liquor from percolation filter top at leisure again, when liquid closes and closes switch in the time that end opening flows out, thereby the liquid flowing out is poured in percolation filter, continue to add Chinese liquor paramount go out till medicated powder face number centimetre, add a cover to place after 24～48 hours and open end opening switch, percolate is slowly flowed out, make described former drug filtrate.
Wherein, the preparation of described N-GEECDCGSPENPCCD:
Get the thick malicious solution of snake venom that the thick malicious lyophilized powder of snake venom is dissolved in deionized water and is made into 40g/L, 18000rpm4 ℃ of centrifugal 30min, gets supernatant, 4 ℃ of preservations;
Sephadex G-75 gel column (φ 55cm X50cm) spends the night with deionized water balance in advance, and snake venom supernatant sample is loaded to the Sephadex G-75 gel column after balance, and applied sample amount is 400mg/10ml;
After completion of the sample, with deionized water eluting, flow velocity is 1.6ml/min; After the thick poisons Sephadex G-75 of snake venom column chromatography, obtain altogether 4 eluting peaks; Collect 4 peaks (finally eluting), obtain described N-GEECDCGSPENPCCD, cryogenic vacuum lyophilizing is for subsequent use.In 4 peaks, consist predominantly of nearly 10 kinds of cells, neural activity polypeptide, one's share of expenses for a joint undertaking amount is all less than 10kDa.
Wherein, the preparation of described Buthotoxin polypeptide:
Get the thick malicious solution of scorpion venom that the thick malicious lyophilized powder of scorpion venom is dissolved in deionized water and is made into 40g/L, 18000rpm4 ℃ of centrifugal 30min, gets supernatant, 4 ℃ of preservations;
Sephadex G-50 gel column (φ 55cm X50cm) spends the night with deionized water balance in advance, and scorpion venom supernatant sample is loaded to the Sephadex G-50 gel column after balance, and applied sample amount is 400mg/10ml;
After completion of the sample, with deionized water eluting, flow velocity is 1.6ml/min; After the thick poisons SephadexG-50 column chromatography of scorpion venom, obtain altogether 3 eluting peaks; Collect 3 peaks (finally eluting), obtain described Buthotoxin polypeptide, cryogenic vacuum lyophilizing is for subsequent use.In 3 peaks, consist predominantly of more than 10 and plant cell, neural activity polypeptide, one's share of expenses for a joint undertaking amount is all less than 10kDa.
The usage of snakes and scorpions wine of the present invention: oral, each serving 50-100 milliliter, day takes 2-3 time.
The present invention includes Radix Cyathulae, Radix Angelicae Pubescentis, Cortex Acanthopancis, Cortex erythrinae, Cortex Cinnamomi, Rhizoma Chuanxiong, Radix Angelicae Sinensis, Radix Codonopsis, Radix Clematidis, Radix Saposhnikoviae, the Cortex Eucommiae, Radix Aconiti, Radix Tripterygii Wilfordii, Semen Strychni (processed), Radix Glycyrrhizae, N-GEECDCGSPENPCCD, Buthotoxin polypeptide, Mel and wine.Wherein said N-GEECDCGSPENPCCD and Buthotoxin polypeptide are the active component that a class is active by force, sphere of action is wide.They and above-mentioned Chinese medicine and wine carry out compatibility, utilize synergism mutual between them, by adopting percolation to be prepared.Not only taking convenience, comfortable taste.And can effectively treat the disease such as rheumatic arthralgia, muscles and bones contracture, manufacture method is simple, evident in efficacy.Function: benefiting QI for activating blood circulation, dispelling wind and removing obstruction in the collateral, analgesia.Cure mainly: the diseases such as disharmony between QI and blood, limbs pain, Feng Shi Paralysis resistance, the contracture of muscle arteries and veins.
Concrete embodiment
Below in conjunction with the specific embodiment, the present invention is described in further detail, understands the present invention to help those skilled in the art.
Embodiment 1
A kind of snakes and scorpions analgesia liquor for treating rheumatism, is obtained by following former medicine preparation: 10 grams of Radix Cyathulaes, 10 grams of Radix Angelicae Pubescentiss, 10 grams of Cortex Acanthopanciss, 10 grams of Cortex erythrinaes, 10 grams of Cortex Cinnamomis, 10 grams of Rhizoma Chuanxiongs, 10 grams of Radix Angelicae Sinensis, 10 grams of Radix Codonopsis, 5 grams of Radix Clematidis, 5 grams of Radix Saposhnikoviaes, 5 grams of the Cortexs Eucommiae, 5 grams of Radix Aconitis, 3 grams of Radix Tripterygii Wilfordiis (band dermatome), 1.5 grams of Semen Strychni (processed)s (husky scalding), 20 grams, Radix Glycyrrhizae, 0.30 gram of N-GEECDCGSPENPCCD, 0.15 gram of Buthotoxin polypeptide, Mel 50ml, Chinese liquor 1500ml(55 ± 3 °).
The preparation method of snakes and scorpions analgesia liquor for treating rheumatism, is specially:
(1), the preparation of N-GEECDCGSPENPCCD:
Get the thick malicious solution of snake venom that the thick malicious lyophilized powder of snake venom is dissolved in deionized water and is made into 40g/L, 18000rpm4 ℃ of centrifugal 30min, gets supernatant, 4 ℃ of preservations;
Sephadex G-75 gel column (φ 55cm X50cm) spends the night with deionized water balance in advance, and snake venom supernatant sample is loaded to the Sephadex G-75 gel column after balance, and applied sample amount is 400mg/10ml;
After completion of the sample, with deionized water eluting, flow velocity is 1.6ml/min; After the thick poisons Sephadex G-75 of snake venom column chromatography, obtain altogether 4 eluting peaks; Collect 4 peaks (finally eluting), obtain described N-GEECDCGSPENPCCD, cryogenic vacuum lyophilizing is for subsequent use.
(2), the preparation of Buthotoxin polypeptide:
Get the thick malicious solution of scorpion venom that the thick malicious lyophilized powder of scorpion venom is dissolved in deionized water and is made into 40g/L, 18000rpm4 ℃ of centrifugal 30min, gets supernatant, 4 ℃ of preservations;
Sephadex G-50 gel column (φ 55cm X50cm) spends the night with deionized water balance in advance, and scorpion venom supernatant sample is loaded to the Sephadex G-50 gel column after balance, and applied sample amount is 400mg/10ml;
After completion of the sample, with deionized water eluting, flow velocity is 1.6ml/min; After the thick poisons Sephadex G-50 of scorpion venom column chromatography, obtain altogether 3 eluting peaks; Collect 3 peaks (finally eluting), obtain described Buthotoxin polypeptide, cryogenic vacuum lyophilizing is for subsequent use.
(3), the preparation of former drug filtrate:
First 15 taste traditional Chinese medicine powders are broken into coarse powder, add appropriate Chinese liquor to infiltrate 2～4 hours, make medicinal material coarse powder fill a part expansion;
Then, gradation packs equably bottom into and is lined with in the percolation filter (percolation filtration extraction pot) of absorbent cotton, installs to compress with wooden stick afterwards at every turn; Install Chinese crude drug, gauze in upper cover, and press the clean handstone of one deck, in order to avoid medicated powder is floated after adding Chinese liquor.
Then open the switch of percolation filter end opening, add Chinese liquor from percolation filter top at leisure again, when liquid closes and closes switch in the time that end opening flows out, thereby the liquid flowing out is poured in percolation filter, continue to add Chinese liquor paramount go out till medicated powder face number centimetre, add a cover to place after 24～48 hours and open end opening switch, percolate is slowly flowed out, make described former drug filtrate.
(4), the preparation of snakes and scorpions analgesia liquor for treating rheumatism:
First the Radix Cyathulae of described amount, Radix Angelicae Pubescentis, Cortex Acanthopancis, Cortex erythrinae, Cortex Cinnamomi, Rhizoma Chuanxiong, Radix Angelicae Sinensis, Radix Codonopsis, Radix Clematidis, Radix Saposhnikoviae, the Cortex Eucommiae, Radix Aconiti, Radix Tripterygii Wilfordii, Semen Strychni (processed), Radix Glycyrrhizae 15 taste traditional Chinese medicine powders are broken into coarser powder, adopt percolation to make former drug filtrate; In former drug filtrate, add the Mel of described amount to stir evenly again, after the standing a few days, leach medicinal liquid; Finally add N-GEECDCGSPENPCCD and the Buthotoxin polypeptide of described amount, after stirring evenly, make described snakes and scorpions analgesia liquor for treating rheumatism.
Embodiment 2
A kind of snakes and scorpions analgesia liquor for treating rheumatism, is obtained by following former medicine preparation: 15 grams of Radix Cyathulaes, 15 grams of Radix Angelicae Pubescentiss, 15 grams of Cortex Acanthopanciss, 5 grams of Cortex erythrinaes, 5 grams of Cortex Cinnamomis, 5 grams of Rhizoma Chuanxiongs, 5 grams of Radix Angelicae Sinensis, 15 grams of Radix Codonopsis, 10 grams of Radix Clematidis, 5 grams of Radix Saposhnikoviaes, 5 grams of the Cortexs Eucommiae, 5 grams of Radix Aconitis, 2 grams of Radix Tripterygii Wilfordiis (band dermatome), 2.4 grams of Semen Strychni (processed)s (husky scalding), 15 grams, Radix Glycyrrhizae, 0.45 gram of N-GEECDCGSPENPCCD, 0.1 gram of Buthotoxin polypeptide, Mel 50ml, Chinese liquor 1500ml(55 ± 3 °).
The preparation method of snakes and scorpions analgesia liquor for treating rheumatism, with embodiment 1.
Embodiment 3
A kind of snakes and scorpions analgesia liquor for treating rheumatism, is obtained by following former medicine preparation: 5 grams of Radix Cyathulaes, 5 grams of Radix Angelicae Pubescentiss, 5 grams of Cortex Acanthopanciss, 10 grams of Cortex erythrinaes, 10 grams of Cortex Cinnamomis, 10 grams of Rhizoma Chuanxiongs, 10 grams of Radix Angelicae Sinensis, 5 grams of Radix Codonopsis, 5 grams of Radix Clematidis, 10 grams of Radix Saposhnikoviaes, 10 grams of the Cortexs Eucommiae, 10 grams of Radix Aconitis, 1.55 grams of Radix Tripterygii Wilfordiis (band dermatome), 3 grams of Semen Strychni (processed)s (husky scalding), 25 grams, Radix Glycyrrhizae, 0.1 gram of N-GEECDCGSPENPCCD, 0.3 gram of Buthotoxin polypeptide, Mel 50ml, Chinese liquor 1500ml(55 ± 3 °).
The preparation method of snakes and scorpions analgesia liquor for treating rheumatism, with embodiment 1.
Embodiment 4
A kind of snakes and scorpions analgesia liquor for treating rheumatism, is obtained by following former medicine preparation: 5 grams of Radix Cyathulaes, 5 grams of Radix Angelicae Pubescentiss, 5 grams of Cortex Acanthopanciss, 15 grams of Cortex erythrinaes, 15 grams of Cortex Cinnamomis, 15 grams of Rhizoma Chuanxiongs, 10 grams of Radix Angelicae Sinensis, 5 grams of Radix Codonopsis, 5 grams of Radix Clematidis, 5 grams of Radix Saposhnikoviaes, 5 grams of the Cortexs Eucommiae, 5 grams of Radix Aconitis, 2.2 grams of Radix Tripterygii Wilfordiis (band dermatome), 2 grams of Semen Strychni (processed)s (husky scalding), 15 grams, Radix Glycyrrhizae, 0.5 gram of N-GEECDCGSPENPCCD, 0.25 gram of Buthotoxin polypeptide, Mel 50ml, Chinese liquor 1500ml(55 ± 3 °).
The preparation method of snakes and scorpions analgesia liquor for treating rheumatism, with embodiment 1.
Above-described embodiment, is preferred embodiment of the present invention, is not used for limiting the scope of the present invention, and the equivalence of being done with the feature described in the claims in the present invention and principle therefore all changes or modifies, within all should being included in the claims in the present invention scope.
The present invention's process is carried out follow-up investigation to more than 30 patients,
Certain man, the age: 45 years old, native place: Guangdong, medical history: knee joint rheumatalgia 5 years
Treatment: oral, each serving 50 milliliters, day takes 2-3 time, takes 15 days, and pain symptom improves, and takes after 3 months, and knee joint closes without pain.
Certain man, the age: 60 years old, native place: Guangdong, medical history: knee joint rheumatalgia 10 years
Treatment: oral, each serving 100 milliliters, day takes 2-3 time, takes 30 days, and pain symptom improves, and takes after 3 months, and knee joint closes without pain.
Certain female, the age: 45 years old, native place: Hunan, medical history: ten articulations digitorum manus rheumatalgia 3 years
Treatment: oral, each serving 50 milliliters, day takes 2-3 time, takes 10 days, and pain symptom improves, and takes after 1 month, and knee joint closes without pain.
Certain man, the age: 70 years old, native place: Guizhou, medical history: have a pain in the leg due to muscles and bones contracture more than 10 years
Treatment: oral, each serving 60 milliliters, day takes 2-3 time, takes 20 days, and the symptom that has a pain in the leg is improved, and takes after 2 months, and lower limb is without pain.
More than just partial results illustrates effect of the present invention, and other does not enumerate.
Claims (10)
1. a snakes and scorpions analgesia liquor for treating rheumatism, is mainly made up of former medicament extract and wine; It is characterized in that, the solid night between described former medicine and wine is than being 1:12～1:13(grams per milliliter);
Described former medicated bag is drawn together the following material according to listed as parts by weight:
0.1～0.3 part of 5～15 parts of Radix Cyathulaes, 5～15 parts of Radix Angelicae Pubescentiss, 5～15 parts of Cortex Acanthopanciss, 5～15 parts of Cortex erythrinaes, 5～15 parts of Cortex Cinnamomis, 5～15 parts of Rhizoma Chuanxiongs, 5～10 parts of Radix Angelicae Sinensis, 5～15 parts of Radix Codonopsis, 5～10 parts of Radix Clematidis, 5～10 parts of Radix Saposhnikoviaes, 5～10 parts of the Cortexs Eucommiae, 5～10 parts of Radix Aconitis, 1～3 part of Radix Tripterygii Wilfordii, 1～3 part of Semen Strychni (processed), 15～25 parts, Radix Glycyrrhizae, 0.1～0.5 part of N-GEECDCGSPENPCCD and Buthotoxin polypeptide.
2. snakes and scorpions analgesia liquor for treating rheumatism according to claim 1, is characterized in that, described former medicated bag is drawn together the following material according to listed as parts by weight:
0.15 part of 10 parts of Radix Cyathulaes, 10 parts of Radix Angelicae Pubescentiss, 10 parts of Cortex Acanthopanciss, 10 parts of Cortex erythrinaes, 10 parts of Cortex Cinnamomis, 10 parts of Rhizoma Chuanxiongs, 10 parts of Radix Angelicae Sinensis, 10 parts of Radix Codonopsis, 5 parts of Radix Clematidis, 5 parts of Radix Saposhnikoviaes, 5 parts of the Cortexs Eucommiae, 5 parts of Radix Aconitis, 3 parts of Radix Tripterygii Wilfordiis, 1.5 parts of Semen Strychni (processed)s, 20 parts, Radix Glycyrrhizae, 0.30 part of N-GEECDCGSPENPCCD and Buthotoxin polypeptide.
3. snakes and scorpions analgesia liquor for treating rheumatism according to claim 1 and 2, is characterized in that, described snakes and scorpions analgesia liquor for treating rheumatism also comprises Mel, and the volume ratio of described Mel and wine is 1:30.
4. snakes and scorpions analgesia liquor for treating rheumatism according to claim 1 and 2, is characterized in that, described Radix Tripterygii Wilfordii band dermatome.
5. snakes and scorpions analgesia liquor for treating rheumatism according to claim 1 and 2, is characterized in that, described Semen Strychni (processed) is processed gained for sand scalds big gun.
6. snakes and scorpions analgesia liquor for treating rheumatism according to claim 1 and 2, is characterized in that, described wine is the Chinese liquor of 55 ± 3 °.
7. the preparation method of snakes and scorpions analgesia liquor for treating rheumatism described in any one in claim 1～6, is characterized in that, is specially:
First the Radix Cyathulae of described amount, Radix Angelicae Pubescentis, Cortex Acanthopancis, Cortex erythrinae, Cortex Cinnamomi, Rhizoma Chuanxiong, Radix Angelicae Sinensis, Radix Codonopsis, Radix Clematidis, Radix Saposhnikoviae, the Cortex Eucommiae, Radix Aconiti, Radix Tripterygii Wilfordii, Semen Strychni (processed), Radix Glycyrrhizae 15 taste traditional Chinese medicine powders are broken into coarser powder, adopt percolation to make former drug filtrate; After leaving standstill a few days, leach medicinal liquid;
Add again N-GEECDCGSPENPCCD and the Buthotoxin polypeptide of described amount, after stirring evenly, make described snakes and scorpions analgesia liquor for treating rheumatism.
8. according to the preparation method of the analgesia of snakes and scorpions described in claim 7 liquor for treating rheumatism, it is characterized in that the preparation of described former drug filtrate:
First Radix Cyathulae, Radix Angelicae Pubescentis, Cortex Acanthopancis, Cortex erythrinae, Cortex Cinnamomi, Rhizoma Chuanxiong, Radix Angelicae Sinensis, Radix Codonopsis, Radix Clematidis, Radix Saposhnikoviae, the Cortex Eucommiae, Radix Aconiti, Radix Tripterygii Wilfordii, Semen Strychni (processed), Radix Glycyrrhizae 15 taste traditional Chinese medicine powders are broken into coarse powder, add appropriate Chinese liquor to infiltrate 2～4 hours, make medicinal material coarse powder fill a part expansion;
Then, gradation packs equably bottom into and is lined with in the percolation filter of absorbent cotton, installs to compress with wooden stick afterwards at every turn;
Then open the switch of percolation filter end opening, add Chinese liquor from percolation filter top at leisure again, when liquid closes and closes switch in the time that end opening flows out, thereby the liquid flowing out is poured in percolation filter, continue to add Chinese liquor paramount go out medicated powder face, add a cover to place after 24～48 hours and open end opening switch, percolate is slowly flowed out, make described former drug filtrate.
9. according to the preparation method of the analgesia of snakes and scorpions described in claim 7 liquor for treating rheumatism, it is characterized in that the preparation of described N-GEECDCGSPENPCCD:
Get the thick malicious solution of snake venom that the thick malicious lyophilized powder of snake venom is dissolved in deionized water and is made into 40g/L, 18000rpm4 ℃ of centrifugal 30min, gets supernatant, 4 ℃ of preservations;
Sephadex G-75 gel column (φ 55cm X50cm) spends the night with deionized water balance in advance, and snake venom supernatant sample is loaded to the Sephadex G-75 gel column after balance, and applied sample amount is 400mg/10ml;
After completion of the sample, with deionized water eluting, flow velocity is 1.6ml/min; After the thick poisons Sephadex G-75 of snake venom column chromatography, obtain altogether 4 eluting peaks; Collect 4 peaks, obtain described N-GEECDCGSPENPCCD, cryogenic vacuum lyophilizing is for subsequent use.
10. according to the preparation method of the analgesia of snakes and scorpions described in claim 7 liquor for treating rheumatism, it is characterized in that the preparation of described Buthotoxin polypeptide:
Get the thick malicious solution of scorpion venom that the thick malicious lyophilized powder of scorpion venom is dissolved in deionized water and is made into 40g/L, 18000rpm4 ℃ of centrifugal 30min, gets supernatant, 4 ℃ of preservations;
Sephadex G-50 gel column (φ 55cm X50cm) spends the night with deionized water balance in advance, and scorpion venom supernatant sample is loaded to the Sephadex G-50 gel column after balance, and applied sample amount is 400mg/10ml;
After completion of the sample, with deionized water eluting, flow velocity is 1.6ml/min; After the thick poisons Sephadex G-50 of scorpion venom column chromatography, obtain altogether 3 eluting peaks; Collect 3 peaks, obtain described Buthotoxin polypeptide, cryogenic vacuum lyophilizing is for subsequent use.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105311074A (en) * | 2014-07-30 | 2016-02-10 | 高晓燕 | Formulation for treating arthralgia |
| CN115006486A (en) * | 2022-05-30 | 2022-09-06 | 刘汉有 | Antirheumatic medicinal liquor and using method thereof |
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| CN1198934A (en) * | 1997-05-09 | 1998-11-18 | 司纪圣 | Medicated wine for curing rheumarthritis and rheumatoid arthritis and its preparation process |
| CN1891233A (en) * | 2005-07-08 | 2007-01-10 | 黄巨轮 | Peptide nutrient liquid |
| CN103555527A (en) * | 2013-10-24 | 2014-02-05 | 重庆师范大学 | Snake peptide healthcare wine and preparation method thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1198934A (en) * | 1997-05-09 | 1998-11-18 | 司纪圣 | Medicated wine for curing rheumarthritis and rheumatoid arthritis and its preparation process |
| CN1891233A (en) * | 2005-07-08 | 2007-01-10 | 黄巨轮 | Peptide nutrient liquid |
| CN103555527A (en) * | 2013-10-24 | 2014-02-05 | 重庆师范大学 | Snake peptide healthcare wine and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105311074A (en) * | 2014-07-30 | 2016-02-10 | 高晓燕 | Formulation for treating arthralgia |
| CN115006486A (en) * | 2022-05-30 | 2022-09-06 | 刘汉有 | Antirheumatic medicinal liquor and using method thereof |
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| CN103861078B (en) | 2018-04-27 |
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