CN103555527A - Snake peptide healthcare wine and preparation method thereof - Google Patents
Snake peptide healthcare wine and preparation method thereof Download PDFInfo
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- CN103555527A CN103555527A CN201310513544.3A CN201310513544A CN103555527A CN 103555527 A CN103555527 A CN 103555527A CN 201310513544 A CN201310513544 A CN 201310513544A CN 103555527 A CN103555527 A CN 103555527A
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Abstract
The invention discloses a snake peptide healthcare wine and a preparation method thereof. The snake peptide healthcare wine is characterized by consisting of the following raw material components in parts by volume: 670-680 parts of distilled spirit, 100-200 parts of a snake venom active peptide solution, 10-20 parts of fructus lycii soak and 110-210 parts of purified water. The preparation method of the snake peptide healthcare wine is characterized by comprising the process steps of (1) preparing the snake venom active peptide solution; (2) preparing the fructus lycii soak; (3) blending; (4) standing and filtering. The invention also discloses a herba houttuyniae scald spraying film agent and a preparation method thereof. The method is simple and convenient, the prepared snake peptide healthcare wine can be quickly and safely absorbed, snake venom can be obtained without killing a poisonous snake, the production cost is low, and the snake peptide healthcare wine can effectively relieve fatigue and arthritis.
Description
Technical field
The invention belongs to health promoting wine technical field, specifically, relate to a kind of snake peptide health promoting wine and preparation method thereof.
Background technology
Along with the quickening of modern life rhythm, the aggravation of social competition, hypoimmunity tired and that cause thus becomes a lot of people's of puzzlement health problem.In addition the aged old man's of China rapid growth, causes elderly population general health level to decline, and senile chronic Non Communicable Diseases (NCD) constantly increases.Therefore, develop the healthcare products that are applicable to this class crowd life-time service and there are wide market outlook.
Snake, as a kind of animal of integration of drinking and medicinal herbs, is all subject to the attention that people are suitable from ancient times to the present.Research shows, snake body, snake body enzymolysis functional polypeptide and snake venom all have the effect of alleviating physical fatigue.Among the people, with snake, steep in wine in the existing long history of China.With snake wine, cure the disease, as far back as Western Zhou period, China working people just starts to drink, and for thousands of years, motherland's medical science is being gained most valuable experience aspect snake wine.Along with improving constantly of living standards of the people, snake wine is more and more subject to people's attention and welcomes.
At present, the snake class health promoting wine product that China's approval is produced, the snake body using is mainly pallas pit viper, point kiss Pallas pit viper, Naja, coral snake, Zaocys etc., wherein has and take full snake as raw material, adds various Chinese medicinal materialss immersions to form, its formula is multifarious, its product efficacy is fuzzy and imprecise, its complicated component, and its functional component is often designated as the glycoside material of Chinese medicinal materials, and and not mentioned snake body component, thereby what more can not illustrate that its product plays efficacy effect is snake body or its component; Also have and directly take snake venom as raw material, add various medicinal materials and obtain by soaking in Chinese liquor, this mode snake venom toxicity is larger, although in the course of processing, the modes such as alcohol and heating can reduce venom toxicity, but the security of its use is still very low; Complete no matter take snake or snake venom is raw material, the specific absorption of its effective constituent is all very low.
Prior art shortcoming: the specific absorption of existing snake class health promoting wine effective constituent is low, and safety in utilization is low, large to the amount of slaughtering of snake, and Production of Health Wine cost is high.
Summary of the invention
For solving above technical problem, the object of the present invention is to provide a kind of effective constituent specific absorption high, use relatively low snake peptide health promoting wine of safety, Production of Health Wine cost and preparation method thereof.
The object of the present invention is achieved like this: a kind of snake peptide health promoting wine, and its key is to be comprised of the material composition of following volume parts:
White wine: 670～680 parts,
Snake venom bioactive peptide solution: 100～200 parts,
Wolfberry fruit soak solution: 10～20 parts,
Purified water: 110～210 parts;
Described wolfberry fruit soak solution is that wolfberry fruit soaks to filter and obtains in white wine.
The preparation of above-mentioned snake venom bioactive peptide solution is comprised of following steps:
1. take echidnotoxin matter, by purified water, fully dissolve, be warming up to 50 ℃ after insulation;
2. the ratio that is 10000U/g according to enzyme/substrate is added proteolytic enzyme and is carried out enzymolysis, stirs, and under the condition of 45 ℃, acts on 6 hours, and stirring per hour once;
3. enzymolysis solution is heated to 80 ℃ of effects 20 minutes, is cooled to room temperature;
4. the enzymolysis solution going out after enzyme is placed in to 8000 revs/min, whizzer, centrifugal 15 minutes, discard precipitation, obtain snake venom bioactive peptide solution.
The preparation method of above-mentioned wolfberry fruit soak solution is: take wolfberry fruit, and soak with white wine, wolfberry fruit is 0.1g/ml with the ratio of white wine consumption, soaks after 30 days, with flame filter press, filters to obtain wolfberry fruit soak solution.
Above-mentioned snake venom is taken from pallas pit viper or/and agkistrodon acutus.
Above-mentioned snake venom is taken from pit viper venom and agkistrodon acutus venom, and the adding proportion of described pit viper venom and agkistrodon acutus venom is 1:1.
A preparation method for snake peptide health promoting wine, its key is to comprise following processing step:
(1) prepare snake venom bioactive peptide solution:
1. take echidnotoxin matter, by purified water, fully dissolve, be warming up to 50 ℃ after insulation;
2. the ratio that is 10000U/g according to enzyme/substrate is added proteolytic enzyme and is carried out enzymolysis, stirs, and under the condition of 45 ℃, acts on 6 hours, during stirring per hour once;
3. enzymolysis solution is heated to 80 ℃ of effects 20 minutes, is cooled to room temperature;
4. the enzymolysis solution going out after enzyme is placed in to 8000 revs/min, whizzer, centrifugal 15 minutes, discard precipitation, obtain snake venom bioactive peptide solution;
(2) prepare wolfberry fruit soak solution: take wolfberry fruit, and soak with white wine, wolfberry fruit is 0.1g/ml with the ratio of white wine consumption, soaks after 30 days, with flame filter press, filters to obtain wolfberry fruit soak solution, saves backup;
(3) allotment: by volume umber joins white wine, wolfberry fruit soak solution, snake venom bioactive peptide solution in blend tank and mixes successively, places after 5 hours, and by volume umber adds purified water, more abundant stirring and evenly mixing;
(4) standing and filtration: standing 12 hours, by flame filter press and 10 microns of membrane filters, filter successively, obtain smart filtrate and be snake peptide health promoting wine;
The preparation method of above-mentioned snake peptide health promoting wine, also comprise the filling screening of step (5): filling front White Spirit Bottle is cleaned with bottle washing machine, inside and outside wall at least rinses 2 times, and hydraulic pressure is 0.10～0.15Mpa, then disinfects in alcohol, dry, bottle cap is soaked in water, and rinsing, uses 75% alcohol disinfecting, dry, then that smart filtrate is filling in White Spirit Bottle with automatic filling machine; White Spirit Bottle after filling and illumination are not less than to filter out wine body before the lamp box of 300Lx muddy, or have the defective snake peptide health promoting wine of suspended substance and the visible impurity of naked eyes, then qualified snake peptide health promoting wine is carried out to package test warehouse-in.
Above-mentioned snake venom is taken from pallas pit viper or/and agkistrodon acutus.
Above-mentioned snake venom is taken from pit viper venom and agkistrodon acutus venom, and wherein the adding proportion of pit viper venom and agkistrodon acutus venom is 1:1.
One, the various index parameters of snake peptide health promoting wine:
1, the Oranoleptic indicator of snake peptide health promoting wine: measure 30ml sample, pour in the clean dry flint glass cup of 50ml, detect by an unaided eye under available light its color and luster and proterties, with its smell of nasil, taste flavour with mouth, reaches shown in table 1:
The requirement of table 1 sense organ
2, physical and chemical index: the physical and chemical index of snake peptide health promoting wine of the present invention is as shown in table 2:
Table 2 physical and chemical index
| Project | Index | Detection method |
| Alcoholic strength (20 ℃)/(%vol) | 35±1 | GB/T15038 |
| Methyl alcohol/(g/100ml)≤ | 0.02 | GB/T5009.48 |
[0040]?
| Total reducing sugar (with glucose meter)/(g/L)≤ | 100 | GB/T15038 |
| Plumbous (in Pb)/(mg/L)≤ | 1 | GB/T5009.12 |
| Manganese (in Mn)/(mg/L)≤ | 1.5 | GB/T5009.90 |
| Arsenic (in As)/(mg/L)≤ | 0.3 | GB/T5009.11 |
| Total acid (with acetometer)/(g/L)≤ | 6.0 | GB/T15038 |
3, microbiological indicator requires: the microbiological indicator of snake peptide health promoting wine of the present invention is as shown in table 3:
Table 3 microbiological indicator
Two, snake peptide health promoting wine is carried out to security detection and result:
1, acute toxicity test
Method and result: snake peptide health promoting wine 200ml/kg.bw dosage per os gives female, male SD rat take after, observe and record toxicity symptom or the untoward reaction of SD rat, after off-test in two weeks, cut open and kill all animals, internal organs show no obvious abnormalities change, illustrate that snake peptide health promoting wine of the present invention is greater than 200ml/kg.bw to acute oral MTD female, male SD rat, belong to non-toxic type, the results are shown in Table 4:
The lopsided Oral toxicity test-results of table 4 snake peptide health promoting wine to SD rat
2, genetic toxicity test
2.1Ames test
Carry out Salmonella reversion test, the results are shown in Table 5 and table 6, result shows: material adds and the test that does not add S9, every ware of spontaneous control group on average returns and becomes colony number all in range of normal value, and every ware that positive controls is brought out on average returns and becomes colony number and be spontaneous control group more than two times, be obvious positive reaction; The evaluation of each test group is returned and is become colony number and be a times over solvent control group, and the reaction that is negative, illustrates that snake peptide health promoting wine of the present invention is without the effect of induction experiment bacterial strain reverse mutation.
Table 5 snake peptide health promoting wine Salmonella reversion test result (for the first time) (individual/ware)
Table 6 snake peptide health promoting wine Salmonella reversion test result (for the second time) (individual/ware)
2.2 mouse polychromatic erythrocytes micronucleus tests
Snake peptide health promoting wine of the present invention negative result in mouse marrow cell micro nuclear test, the results are shown in Table 7, result shows: the micronuclear rates of the female tom of positive controls is all significantly higher than negative control group (P < 0.01), micronuclear rates of each group of tested material and negative control group comparison there are no significant difference (P > 0.05).
The impact of table 7 snake peptide health promoting wine on mouse Bone marrow cells micronucleus incidence
Note: compare * *: P<0.01 with negative control
2.3 mouse sperm deformity tests
Carry out mouse sperm deformity test, snake peptide health promoting wine of the present invention is negative result in the test of mouse deformity, the results are shown in Table 8, result shows: the rate of teratosperm of positive controls is significantly higher than negative control group (P < 0.01), the rate of teratosperm of each dosage group and negative control group comparison there are no significant difference (P > 0.05).
Table 8 snake peptide health promoting wine is to mouse sperm deformity test-results
Note: compare * *: P<0.01 with negative control; △ comprises double end, and two tails are fat first-class.
2.4 rat 30 days feeding studys
Negative control group and three dosage treated animals, during within 30 days, feeding, are ingested, drinking-water, stool and urine is normal, and the situation of growing and general performance are good, do not observe evident act and change and poisoning manifestations.
2.4.1 the impact on body weight and food utilization
Each experimental group at the rat body weight of whole experimental session, the measurement result of male and female rat food utilization respectively as shown in Table 9 and Table 10, the male mouse high dose group of animal subject animal ingests and slightly increases (P < 0.05) on the 2nd week, female book base wine treated animal the 3rd axle is ingested and is slightly reduced (P < 0.05), but female tom total foodstuff utilizes with control group and compares there was no significant difference (P > 0.05), snake peptide health promoting wine of the present invention is described, and to body growth, growth has no adverse effects.
The impact of table 9 snake peptide health promoting wine on rat body weight
result
The affect result of table 10 snake peptide health promoting wine on rat food utilization
Note: compare *: p<0.05 with negative control.
2.4.2 organ coefficient measurement result:
The weight of the important organ of each dosage group of snake peptide health promoting wine and organ coefficient measurement result are as shown in table 11 and draw: female mouse high dose group spleen weight in wet base is compared with control group high (P < 0.05), the weight of each dosage group important organ of all the other male and female mouse and organ coefficient and negative control group relatively there are no significant difference (P > 0.05).
Table 11 snake peptide health promoting wine on Rats Organs and Tissues weight and coefficient affect result (n=10,
)
Note: compare *: p<0.05 with negative control.
2.4.3 latter stage blood test result:
Measurement result is as shown in table 12 and draw for the conventional hematological indices (red corpuscle, oxyphorase, platelet) in test latter stage: male mouse base wine combination low dose group animal RBC slightly reduces (P < 0.05), three dosage group RBC of female mouse all slightly raise (P < 0.05) compared with control group, all the other indexs and negative control group relatively there are no significant difference.
Table 12 snake peptide health promoting wine is learned the result that affects of index on rat blood
Notes 1:RBC is red corpuscle, and Hb is erythrocyte, and WBC is white corpuscle, and GR is neutrophil leucocyte, and LY is lymphocyte; Note 2: with negative control to relatively, P < 0.05.
2.4.4 latter stage biochemical indicator detected result:
Each experimental group Biochemical Indices In Serum is as shown in table 13 and draw: in female mouse, dosage group Cr reduces (P < 0.05) compared with control group, abiology meaning, all the other each dosage groups of index male and female mouse and negative control group relatively there are no significant difference (P > 0.05).
Table 13 snake peptide wine is on the impact of rat blood serum biochemical indicator (x ± s, n=10) result
Notes 1:ALT is gpt, and AST is glutamic-oxal(o)acetic transaminase, and TP is total protein, and ALB is albumin, and BUN is blood urea nitrogen, and CR is creatinine, and GLU is blood sugar, and CHO is cholesterol, and TG is triglyceride level;
Note 2: with negative control comparison, P<0.05.
2.4.5 histology result: off-test is that liver, kidney, stomach, spleen and jejunum are checked and histopathological examination; Result is respectively as shown in table 14,15,16,17,18.
Table 14 snake peptide health promoting wine rat is fed experiment liver histological observations for 30 days
Table 15 snake peptide health promoting wine wine rat is fed experiment nephridial tissue for 30 days and observes
Table 16 snake peptide health promoting wine is learned and is observed 30 days feeding study stomach-tissues of rat
The histological observation of table 17 snake peptide health promoting wine to 30 days feeding study spleens of rat
Table 18 snake peptide health promoting wine is to 30 days feeding study jejunum histological observations of rat
Result shows: each 1 example of blank combination high dose group kidney interstitial nephritis, and incidence is 5%, but base wine group kidney interstitial nephritis number of cases is more, has 5 examples, and incidence accounts for 25%; Steatosis that liver is slight; Each 1 example of blank group and high dose group belongs to the spontaneous pathology of animal, and base wine group shows surperficial base wine has mild damage to act on to kidney.
In sum, each dosage group of snake peptide health promoting wine of the present invention is showed no to animal viscera the damaging being caused by it and changes.
Three, efficacy detection and result thereof
1, snake peptide health promoting wine is measured for alleviating physical fatigue effect: adopt 60 of SPF level male ICR mouses, and body weight 18～22g, by Chongqing, Teng Xin Bioisystech Co., Ltd provides.Snake peptide health promoting wine is pressed 10 of human body recommended dose, 20, 30 multiple doses, 60 small white mouses are divided into 6 groups at random, every group 10, be snake peptide health promoting wine low dose group (snake peptide health promoting wine 8.3ml/kg), middle dosage group (snake peptide health promoting wine 16.6ml/kg), high dose group (snake peptide health promoting wine 33.2ml/kg), positive controls (rhodioside 180mgkgd) E83, base wine control group (15% wine base), negative control group (distilled water), gavage is 30 days continuously, measure the mice burden swimming time, blood lactic acid and blood urea nitrogen, result is respectively as table 19, 20, shown in 21:
(1) as seen from Table 19, the swimming time of the snake peptide health promoting wine group mouse of 3 kinds of dosage groups is all higher than negative control group and base wine control group, and middle and high dosage group obvious difference (P<0.05), shows that swimming time has concentration dependent to snake peptide health promoting wine; High dose group mouse swimming time and positive controls approach.
The impact of table 19 snake peptide health promoting wine on the mice burden swimming time
| Group | Number of animals | Swimming time (min) |
| Negative control group | 10 | 6.41±0.22 |
| Base wine control group | 10 | 6.59±1.33 |
| Snake peptide health promoting wine low dose group | 10 | 7.50±0.56*# |
[0111]?
| Dosage group in snake peptide health promoting wine | 10 | 8.20±1.31*# |
| Snake peptide health promoting wine high dose group | 10 | 10.55±0.54*# |
| Positive controls | 10 | 13.42±1.30*# |
Note: with the comparison of wine base control group, *: P < 0.05; With negative control group comparison, #:P < 0.05.
(2) as seen from Table 20, the mouse of low, middle dosage snake peptide health promoting wine group is starkly lower than base wine control group and negative control group (P<0.05), and high dosage snake peptide health promoting wine group and base wine control group, negative control group comparing difference have remarkable statistical significance (P < 0.01).
The impact of table 20 snake peptide health promoting wine on Mouse Blood lactic acid (LA) content after swimming
| Group | Number of animals | Lactic acid content (mmol/L) |
| Negative control group | 10 | 34.06±1.33 |
| Base wine control group | 10 | 33.65±2.65 |
| Snake peptide health promoting wine low dose group | 10 | 20.56±1.98*# |
| Dosage group in snake peptide health promoting wine | 10 | 16.87±2.34*# |
| Snake peptide health promoting wine high dose group | 10 | 9.35±1.78** |
| Positive controls | 10 | 9.60±2.54** |
Note: with the comparison of wine base control group, *: P < 0.05; With negative control group comparison, #:P < 0.05; With base wine control group and negative control group comparison, * *: P < 0.01.
(3) as seen from Table 21, the BUN content of snake peptide health promoting wine group mouse reduces gradually with the increase of dosage, and middle and high dosage group and negative control group and base wine control group comparing difference have remarkable statistical significance (P<O.05).
Table 21 snake peptide health promoting wine exhausts the impact of rear Mouse Blood blood urea nitrogen (BUN) content of swimming on power
| Group | Number of animals | Lactic acid content (mmol/L) |
[0120]?
| Negative control group | 10 | 182.951±20.23 |
| Base wine control group | 10 | 180.562±16.52 |
| Snake peptide health promoting wine low dose group | 10 | 175.352±13.79 |
| Dosage group in snake peptide health promoting wine | 10 | 171.521±15.34* |
| Snake peptide health promoting wine high dose group | 10 | 160.203±10.33* |
| Positive controls | 10 | 155.239±19.54*# |
Note: with the comparison of wine base control group, *: P < 0.05; With negative control group comparison, #:P < 0.01.
(4) in sum: snake peptide health promoting wine has the disposable swimming time of obvious prolongation mouse, the content of blood lactate level and BUN is starkly lower than base wine control group and negative control group, illustrate that snake peptide health promoting wine can be by the generation of lactic acid in minimizing campaign, the katabolism of protein while reducing motion, reduce the generation of serum urea nitrogen, reduce the ammonia toxic action that metabolism produces simultaneously, make mouse more can adapt to high-intensity motion, reach the effect of delaying the emergence of fatigue.
2, snake peptide health promoting wine is measured for releasing arthritis effect:
With adjuvant-induced arthritis (adjunctive arthritis, AA) mouse is model, choose 60 of healthy male adult mouse, body weight 20 ± 2g, 60 mouse are divided into 6 groups at random, be control group, model group, wine base group, snake peptide low dose group and high dose group, positive controls, 101 every group.Except control group injecting normal saline, at other, respectively organize the right back sufficient sole of the foot intradermal injection Freund's complete adjuvant of mouse (FCA) 0.02mL induction inflammation and occur, obtain model: adjuvant-induced arthritis (adjunctive arthritis, AA) mouse.After modeling success the 2nd day to 34 days, start mouse to carry out gavage every mouse every day 1 time, each 0.2mL.Positive controls is carried out gavage with 1mg/kg dexamethasone, and wine base group is pressed 10mL/kg gavage, and snake peptide health promoting wine group is pressed respectively 8.3mL/kg(low dosage), 33.2mL/kg(high dosage) gavage, control group and model group are carried out gavage with pure water to mouse.Then the mensuration to AA mouse toes swelling, ankle joint, body weight and CIC.
(1) mensuration of AA mouse toes and ankle swelling degree
Within the 2nd, 10,18,26,34 days after modeling, with electronics vernier callipers, measure and respectively organize mouse right hind toes and ankle joint diameter.Result is as shown in table 22 and 23:
Table 22 snake peptide health promoting wine on the impact of the right back toes swelling of AA mouse (mm,
n=10)
Wherein a:P < 0.05, and b:P < 0.01, with model group comparison; C:P < 0.01, with control group comparison.
Table 23 snake peptide health promoting wine on AA mouse after ankle swelling degree impact (mm,
n=10)
Wherein a:P < 0.05, and b:P < 0.01, with model group comparison; C:P < 0.01, with control group comparison.
From table 22, the 2nd day from modeling starts, and after other respectively organize mouse, toes swelling is significantly higher than control group (P < 0.01).With model group comparison, within the 17th day from administration, be to start for after modeling the 18th day, the swelling of positive controls mouse toes obviously reduces (P < 0.05); The 26th day from modeling starts, and snake peptide low dose group mouse toes swelling significantly reduces (P < 0.01); Wine base group and snake peptide high dose group are all without noticeable change.
From table 23, the 2nd day from modeling starts, and other are respectively organized the right back ankle swelling degree of mouse and are significantly higher than control group (P < 0.01).After modeling the 18th day, positive controls mouse ankle swelling degree significantly reduced (P < 0.05); After modeling the 26th day, snake peptide low dose group, snake peptide high dose group, wine base group and positive controls mouse ankle swelling degree all significantly reduced (P < 0.05); After modeling the 34th day, positive controls, snake peptide high dose group mouse ankle swelling degree all significantly reduce (P < 0.05), snake peptide low dose group and wine base group mouse ankle swelling degree reduce, and difference has statistical significance (P < 0.01).
(2) mensuration of Mice Body quality and index and spleen index
The 2nd, 10,18,26,34 natural gift another name amount Mice Body quality after modeling.After last administration 24h, Weighing body quality, result is shown in table 24:
Table 24 snake peptide health promoting wine on the impact of AA Mice Body quality (mm,
n=10)
Wherein a:P < 0.05, with positive controls comparison.
From table 24, from modeling the 18th day, with control group comparison, positive controls Weight gain was slow, but does not present significant difference; But snake peptide low dose group difference has statistical significance (P < 0.05), when the 26th day and the 34th day, positive controls and the comparison of snake peptide low dose group, difference has statistical significance (P < 0.05).
(3) mensuration of CIC
After modeling the 34th day, eyeball of mouse was got blood, centrifugal (3000r/min, 10min) separation of serum, 2 times of volume 0.1mol/L borate buffers (pH=8.4) diluted for use for serum.Measure pipe and respectively add dilute serum 30 μ L with control tube, measure pipe and add 4.1% polyglycol solution 300 μ L, control tube adds above-mentioned borate buffer 300 μ L, mixes latter 4 ℃ and places 1h.By microplate reader, with 495nm wavelength, measure and respectively manage absorbancy (A) value, detected result is expressed as measures pipe A495nm-control tube 495nm.
Result: after modeling the 34th day, to measure and respectively organize mice serum CIC index, result display model group (2.38 ± 1.22) mice serum CIC index is significantly higher than control group (0.52 ± 0.10) (P < 0.05); Compare with model group, the Mouse Blood CIC index of snake peptide low dose group (0.65 ± 0.14) and positive controls (0.37 ± 0.09) all significantly reduces (P < 0.05).
By found that: only snake peptide health promoting wine low dose group and positive controls mouse CIC significantly reduce, and revert to normal level.
(4) in sum, low dosage snake peptide wine and dexamethasone all have significant result for the treatment of to AA improvement of adjuvant arthritis in mice, but low dosage snake peptide wine result for the treatment of is better, and have no untoward reaction.
Beneficial effect:
A kind of Herba Houttuyniae of the present invention is scalded spray-filming agent and preparation method thereof, and method is simple and convenient, and the snake peptide health promoting wine preparing absorbs fast, safety, need not slaughter poisonous snake to obtain snake venom, and production cost is low, effectively alleviating fatigue and sacroiliitis.
Embodiment
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1: a kind of snake peptide health promoting wine, is characterized in that being comprised of the material composition of following volume parts:
White wine: 680ml,
Snake venom bioactive peptide solution: 200ml,
Wolfberry fruit soak solution: 10ml,
Purified water: 110ml.
A preparation method for snake peptide health promoting wine, comprises following processing step:
(1) prepare snake venom bioactive peptide solution:
1. take viper venom protein 800g, by the purified water of 200ml, fully dissolve, be warming up to 50 ℃ after insulation;
2. the ratio that is 10000U/g according to enzyme/substrate is added proteolytic enzyme and is carried out enzymolysis, stirs, and under the condition of 45 ℃, acts on 6 hours, during stirring per hour once;
3. enzymolysis solution is heated to 80 ℃ of effects 20 minutes, is cooled to room temperature;
4. the enzymolysis solution going out after enzyme is placed in to 8000 revs/min, whizzer, centrifugal 15 minutes, discard precipitation, obtain viper venom bioactive peptide solution, save backup;
(2) prepare wolfberry fruit soak solution: take wolfberry fruit, and soak with 52% aromatic Chinese spirit, wolfberry fruit is 0.1g/ml with the ratio of white wine consumption, soaks after 30 days, with flame filter press, filters to obtain wolfberry fruit soak solution, saves backup;
(3) allotment: successively white wine 680ml, wolfberry fruit soak solution 10ml, viper venom bioactive peptide solution 200ml are joined in blend tank and mixed, place after 5 hours, add 110ml purified water, more abundant stirring and evenly mixing;
(4) standing and filtration: standing 12 hours, by flame filter press and 10 microns of membrane filters, filter successively, obtain smart filtrate and be snake peptide health promoting wine;
(5) filling screening: filling front White Spirit Bottle is cleaned with bottle washing machine, inside and outside wall rinses 4 times, and hydraulic pressure is 0.10～0.15Mpa, then disinfects in alcohol, dry, bottle cap is soaked in water, and is rinsing, use 75% alcohol disinfecting, dry, then that smart filtrate is filling in White Spirit Bottle with automatic filling machine; White Spirit Bottle after filling and illumination are not less than to filter out wine body before the lamp box of 300Lx muddy, or have the defective snake peptide health promoting wine of suspended substance and the visible impurity of naked eyes, then qualified snake peptide health promoting wine is carried out to package test warehouse-in.
Embodiment 2: a kind of snake peptide health promoting wine, is characterized in that being comprised of the material composition of following volume parts:
White wine: 670ml,
Snake venom bioactive peptide solution: 100ml,
Wolfberry fruit soak solution: 20ml,
Purified water: 210ml;
A preparation method for snake peptide health promoting wine, comprises following processing step:
(1) prepare snake venom bioactive peptide solution:
1. take agkistrodon acutus echidnotoxin matter 400g, by the purified water of 100ml, fully dissolve, be warming up to 50 ℃ after insulation;
2. the ratio that is 10000U/g according to enzyme/substrate is added proteolytic enzyme and is carried out enzymolysis, stirs, and under the condition of 45 ℃, acts on 6 hours, during stirring per hour once;
3. enzymolysis solution is heated to 80 ℃ of effects 20 minutes, is cooled to room temperature;
4. the enzymolysis solution going out after enzyme is placed in to 8000 revs/min, whizzer, centrifugal 15 minutes, discard precipitation, obtain agkistrodon acutus snake venom bioactive peptide solution, save backup;
(2) prepare wolfberry fruit soak solution: take wolfberry fruit, and soak with 52% aromatic Chinese spirit, wolfberry fruit is 0.1g/ml with the ratio of white wine consumption, soaks after 30 days, with flame filter press, filters to obtain wolfberry fruit soak solution, saves backup;
(3) allotment: successively white wine 670ml, wolfberry fruit soak solution 20ml, agkistrodon acutus snake venom bioactive peptide solution 100ml are joined in blend tank and mixed, place after 5 hours, add 210ml purified water, more abundant stirring and evenly mixing;
(4) standing and filtration: standing 12 hours, by flame filter press and 10 microns of membrane filters, filter successively, obtain smart filtrate and be snake peptide health promoting wine;
(5) filling screening: filling front White Spirit Bottle is cleaned with bottle washing machine, inside and outside wall rinses 2 times, and hydraulic pressure is 0.10～0.15Mpa, then disinfects in alcohol, dry, bottle cap is soaked in water, and is rinsing, use 75% alcohol disinfecting, dry, then that smart filtrate is filling in White Spirit Bottle with automatic filling machine; White Spirit Bottle after filling and illumination are not less than to filter out wine body before the lamp box of 300Lx muddy, or have the defective snake peptide health promoting wine of suspended substance and the visible impurity of naked eyes, then qualified snake peptide health promoting wine is carried out to package test warehouse-in.
Embodiment 3: a kind of snake peptide health promoting wine, is characterized in that being comprised of the material composition of following volume parts:
White wine: 675ml,
Snake venom bioactive peptide solution: 150ml,
Wolfberry fruit soak solution: 15ml,
Purified water: 160ml.
A preparation method for snake peptide health promoting wine, comprises following processing step:
(1) prepare snake venom bioactive peptide solution:
1. take viper venom protein and each 400g of agkistrodon acutus echidnotoxin matter, after mixing, with the purified water of 200ml, fully dissolves, be incubated after being warming up to 50 ℃;
2. the ratio that is 10000U/g according to enzyme/substrate is added proteolytic enzyme and is carried out enzymolysis, stirs, and under the condition of 45 ℃, acts on 6 hours, during stirring per hour once;
3. enzymolysis solution is heated to 80 ℃ of effects 20 minutes, is cooled to room temperature;
4. the enzymolysis solution going out after enzyme is placed in to 8000 revs/min, whizzer, centrifugal 15 minutes, discard precipitation, obtain snake venom bioactive peptide solution;
(2) prepare wolfberry fruit soak solution: take wolfberry fruit, and soak with 52% aromatic Chinese spirit, wolfberry fruit is 0.1g/ml with the ratio of white wine consumption, soaks after 30 days, with flame filter press, filters to obtain wolfberry fruit soak solution, saves backup;
(3) allotment: successively white wine 675ml, wolfberry fruit soak solution 15ml, snake venom bioactive peptide solution 150ml are joined in blend tank and mixed, place after 5 hours, add 160ml purified water, more abundant stirring and evenly mixing;
(4) standing and filtration: standing 12 hours, by flame filter press and 10 microns of membrane filters, filter successively, obtain smart filtrate and be snake peptide health promoting wine;
(5) filling screening: filling front White Spirit Bottle is cleaned with bottle washing machine, inside and outside wall rinses 3 times, and hydraulic pressure is 0.10～0.15Mpa, then disinfects in alcohol, dry, bottle cap is soaked in water, and is rinsing, use 75% alcohol disinfecting, dry, then that smart filtrate is filling in White Spirit Bottle with automatic filling machine; White Spirit Bottle after filling and illumination are not less than to filter out wine body before the lamp box of 300Lx muddy, or have the defective snake peptide health promoting wine of suspended substance and the visible impurity of naked eyes, then qualified snake peptide health promoting wine is carried out to package test warehouse-in.
Claims (9)
1. a snake peptide health promoting wine, is characterized in that being comprised of the material composition of following volume parts:
White wine: 670～680 parts,
Snake venom bioactive peptide solution: 100～200 parts,
Wolfberry fruit soak solution: 10～20 parts,
Purified water: 110～210 parts;
Described wolfberry fruit soak solution is that wolfberry fruit soaks to filter and obtains in white wine.
2. snake peptide health promoting wine according to claim 1, is characterized in that the preparation of described snake venom bioactive peptide solution is comprised of following steps:
1. take echidnotoxin matter, by purified water, fully dissolve, be warming up to 50 ℃ after insulation;
2. the ratio that is 10000U/g according to enzyme/substrate is added proteolytic enzyme and is carried out enzymolysis, stirs, and under the condition of 45 ℃, acts on 6 hours, and stirring per hour once;
3. enzymolysis solution is heated to 80 ℃ of effects 20 minutes, is cooled to room temperature;
4. the enzymolysis solution going out after enzyme is placed in to 8000 revs/min, whizzer, centrifugal 15 minutes, discard precipitation, obtain snake venom bioactive peptide solution.
3. snake peptide health promoting wine according to claim 1 and 2, the preparation method who it is characterized in that described wolfberry fruit soak solution is: take wolfberry fruit, and soak with white wine, wolfberry fruit is 0.1g/ml with the ratio of white wine consumption, soak after 30 days, with flame filter press, filter to obtain wolfberry fruit soak solution.
4. snake peptide health promoting wine according to claim 3, is characterized in that: described snake venom is taken from pallas pit viper or/and agkistrodon acutus.
5. snake peptide health promoting wine according to claim 4, is characterized in that: described snake venom is taken from pit viper venom and agkistrodon acutus venom, and the adding proportion of described pit viper venom and agkistrodon acutus venom is 1:1.
6. a preparation method for snake peptide health promoting wine claimed in claim 1, is characterized in that comprising following processing step:
(1) prepare snake venom bioactive peptide solution:
1. take echidnotoxin matter, by purified water, fully dissolve, be warming up to 50 ℃ after insulation;
2. the ratio that is 10000U/g according to enzyme/substrate is added proteolytic enzyme and is carried out enzymolysis, stirs, and under the condition of 45 ℃, acts on 6 hours, during stirring per hour once;
3. enzymolysis solution is heated to 80 ℃ of effects 20 minutes, is cooled to room temperature;
4. the enzymolysis solution going out after enzyme is placed in to 8000 revs/min, whizzer, centrifugal 15 minutes, discard precipitation, obtain snake venom bioactive peptide solution;
(2) prepare wolfberry fruit soak solution: take wolfberry fruit, and soak with white wine, wolfberry fruit is 0.1g/ml with the ratio of white wine consumption, soaks after 30 days, with flame filter press, filters to obtain wolfberry fruit soak solution, saves backup;
(3) allotment: by volume umber joins white wine, wolfberry fruit soak solution, snake venom bioactive peptide solution in blend tank and mixes successively, places after 5 hours, and by volume umber adds purified water, more abundant stirring and evenly mixing;
(4) standing and filtration: standing 12 hours, by flame filter press and 10 microns of membrane filters, filter successively, obtain smart filtrate and be snake peptide health promoting wine.
7. the preparation method of snake peptide health promoting wine according to claim 6, characterized by further comprising the filling screening of step (5): filling front White Spirit Bottle is cleaned with bottle washing machine, inside and outside wall at least rinses 2 times, and hydraulic pressure is 0.10～0.15Mpa, then disinfects in alcohol, dry, bottle cap is soaked in water, and rinsing, uses 75% alcohol disinfecting, dry, then that smart filtrate is filling in White Spirit Bottle with automatic filling machine; White Spirit Bottle after filling and illumination are not less than to filter out wine body before the lamp box of 300Lx muddy, or have the defective snake peptide health promoting wine of suspended substance and the visible impurity of naked eyes, then qualified snake peptide health promoting wine is carried out to package test warehouse-in.
8. the preparation method of snake peptide health promoting wine according to claim 6, is characterized in that: described snake venom is taken from pallas pit viper or/and agkistrodon acutus.
9. the preparation method of snake peptide health promoting wine according to claim 7, is characterized in that: described snake venom is taken from pit viper venom and agkistrodon acutus venom, and wherein the adding proportion of pit viper venom and agkistrodon acutus venom is 1:1.
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| CN103555527B (en) | 2014-12-31 |
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