CN103858804B - The breeding method of one pearl polychrome pearl - Google Patents
The breeding method of one pearl polychrome pearl Download PDFInfo
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- CN103858804B CN103858804B CN201410122901.8A CN201410122901A CN103858804B CN 103858804 B CN103858804 B CN 103858804B CN 201410122901 A CN201410122901 A CN 201410122901A CN 103858804 B CN103858804 B CN 103858804B
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- PLXMOAALOJOTIY-FPTXNFDTSA-N Aesculin Natural products OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)[C@H]1Oc2cc3C=CC(=O)Oc3cc2O PLXMOAALOJOTIY-FPTXNFDTSA-N 0.000 title claims abstract description 41
- 238000009395 breeding Methods 0.000 title claims abstract description 25
- 235000015170 shellfish Nutrition 0.000 claims abstract description 84
- 210000001519 tissue Anatomy 0.000 claims abstract description 64
- 238000000034 method Methods 0.000 claims abstract description 25
- 210000002808 connective tissue Anatomy 0.000 claims abstract description 19
- 239000007943 implant Substances 0.000 claims abstract description 18
- 210000005257 cortical tissue Anatomy 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 230000008569 process Effects 0.000 claims description 15
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 9
- 241000237536 Mytilus edulis Species 0.000 claims description 7
- 239000013505 freshwater Substances 0.000 claims description 7
- 235000020638 mussel Nutrition 0.000 claims description 7
- 241000237519 Bivalvia Species 0.000 claims description 6
- 241000206751 Chrysophyceae Species 0.000 claims description 5
- 241000195493 Cryptophyta Species 0.000 claims description 5
- 239000002504 physiological saline solution Substances 0.000 claims description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 3
- 241000382825 Cristaria plicata Species 0.000 claims description 3
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 claims description 3
- 229930182566 Gentamicin Natural products 0.000 claims description 3
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 3
- 241000879903 Pteria Species 0.000 claims description 3
- 241000183300 Pteria penguin Species 0.000 claims description 3
- 241001464055 Pteriidae Species 0.000 claims description 3
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 3
- 239000011575 calcium Substances 0.000 claims description 3
- 229910052791 calcium Inorganic materials 0.000 claims description 3
- 229960002518 gentamicin Drugs 0.000 claims description 3
- 229910052742 iron Inorganic materials 0.000 claims description 3
- 239000011777 magnesium Substances 0.000 claims description 3
- 229910052749 magnesium Inorganic materials 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 239000011593 sulfur Substances 0.000 claims description 3
- 229910052717 sulfur Inorganic materials 0.000 claims description 3
- 238000010923 batch production Methods 0.000 abstract description 3
- 230000008859 change Effects 0.000 abstract description 3
- 239000011049 pearl Substances 0.000 description 142
- 239000000047 product Substances 0.000 description 33
- 230000028327 secretion Effects 0.000 description 6
- 239000003086 colorant Substances 0.000 description 5
- 241000382840 Hyriopsis cumingii Species 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 230000035764 nutrition Effects 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 241000217379 Unionidae Species 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 235000007926 Craterellus fallax Nutrition 0.000 description 1
- 240000007175 Datura inoxia Species 0.000 description 1
- 241000237852 Mollusca Species 0.000 description 1
- 244000225942 Viola tricolor Species 0.000 description 1
- 235000004031 Viola x wittrockiana Nutrition 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000002224 dissection Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009399 inbreeding Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
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- 230000001869 rapid Effects 0.000 description 1
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- 238000009394 selective breeding Methods 0.000 description 1
- 210000004872 soft tissue Anatomy 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention relates to pearl and cultivate field, the particularly breeding method of an a kind of pearl polychrome pearl, comprise the following steps: cultivate multiple first product pearl shellfish with different nacre color; Gather the cortical tissue on the outer embrane of all first product pearl shellfishes respectively, obtain multiple tissue; All tissue are merged, obtains treating transplanted tissue; To treat that transplanted tissue implants in the second pallial connective tissue producing pearl shellfish or internal sac, and cultivate, obtain a pearl polychrome pearl.This method provided by the invention has thoroughly overturned eternal law of an a kind of color of pearl, and make pearl sector produce historic shift, a pearl polychrome, changes the original concept of pearl completely, is the revolutionary achievement of pearl research field.The batch production of " a pearl polychrome " pearl, will thoroughly change pearl market to the appraisement system of pearl quality and world's pearl sector general layout.
Description
Technical field
The present invention relates to pearl and cultivate field, in particular to the breeding method of an a kind of pearl polychrome pearl.
Background technology
The most important condition that pearl is formed generates at product pearl shellfish soft tissue position (connective tissue etc.) to have to internal secretion nacreous " pearl sac "; Before traditional free pearl production method will trace back to more than 100 year, Japanese sees the mantle tissue patch-graft method of rapids occasion equality, and this method generates the technology of pearl sac after the whole world is universal, becomes the basic technology of the modern artificial pearl culture adopted so far.
But in the related, the pearl homogeneous that traditional pearl cultivating method produces only possesses a kind of color (as white, golden, redness, yellow or purple etc.); Because its color is single, tone is simple; Therefore, business demand can not be met.
Summary of the invention
The object of the present invention is to provide the breeding method of an a kind of pearl polychrome pearl, to solve the above problems.
Provide the breeding method of an a kind of pearl polychrome pearl in an embodiment of the present invention, comprise the following steps:
Cultivate multiple first product pearl shellfish with different nacre color;
Gather the cortical tissue on the outer embrane of all described first product pearl shellfishes respectively, obtain multiple tissue;
All described tissue are merged, obtains treating transplanted tissue;
Treat that transplanted tissue implants in the second pallial connective tissue producing pearl shellfish or internal sac by described, and cultivate, obtain a pearl polychrome pearl.
The breeding method of the pearl polychrome pearl that the embodiment of the present invention provides, first it cultivated the first product pearl shellfish with different nacre color, and multiple first produces pearl shellfishes can secrete the nacre producing different colours; So, by gathering the cortical tissue on the outer embrane of all first product pearl shellfishes, obtain multiple tissue, multiple tissue can obtain tissue (its similar pearl sac) to be transplanted by cell chulture again, this tissue possesses the nacreous characteristic of secretion different colours, therefore, is implanted in the second pallial connective tissue producing pearl shellfish or internal sac, and cultivate, a pearl polychrome pearl can be obtained.
Compared with prior art, the breeding method of a this pearl polychrome pearl provided by the invention, it can produce polychrome pearl, the kind of the first product pearl shellfish specifically can be selected according to required color, polychrome pearl rich color, there is higher enjoying value, therefore, can well business demand be met.
This method that the embodiment of the present invention provides thoroughly has overturned eternal law of an a kind of color of pearl, and make pearl sector produce historic shift, a pearl polychrome, changes the original concept of pearl completely, is the revolutionary achievement of pearl research field.The batch production of " a pearl polychrome " pearl, will thoroughly change pearl market to the appraisement system of pearl quality and world's pearl sector general layout.
Optionally, described first product pearl shellfish comprises: hydriopsis cumingii, pond dish freshwater mussel, cristaria plicata, anodon, beautiful freshwater mussel, large nacre, pteria martensii or Pteria penguin.
Described second produces pearl shellfish comprises: described second produces pearl shellfish comprises: the kind of Mollusca Bivalvia Eulamellibranchia Margaritanidae, Unionidae or Mollusca Bivalvia Anisomyaria Pteriidae.
Optionally, in the step of the multiple first product pearl shellfish with different nacre color of described cultivation,
Described first product pearl shellfish is at least through the seed selection of 4 generations, and can secrete the nacreous shellfish of pure color.
Optionally, cortical tissue on the described outer embrane gathering all described first product pearl shellfishes respectively, obtain in the step of multiple tissue, specifically comprise: long for the shell multiple first product pearl shellfishes for 7-10 centimetre are dissected, gather the epidermal tissue on described first product pearl shellfish jacket membrane respectively, and cut into massive texture; Utilize the mixed liquor containing physiological saline, trypsase and gentamicin to maintain all massive textures, obtain the tissue containing cell mass.
Optionally, treat that transplanted tissue implants in the second pallial connective tissue producing pearl shellfish or internal sac described by described, and cultivate, obtain in the step of a pearl polychrome pearl, specifically comprise: by described treat that transplanted tissue implants the second pallial connective tissue producing pearl shellfish or internal sac while, also in the pallial connective tissue or internal sac of described second product pearl shellfish, inserting strain core, by cultivating, obtaining a pearl polychrome pearl.
Optionally, treat that transplanted tissue implants in the second pallial connective tissue producing pearl shellfish or internal sac described by described, and cultivate, obtain in the step of a pearl polychrome pearl, described cultivation specifically comprises: need described second of transplanted tissue produce pearl shellfish hanging culture 2-5 in water by implanting.
Optionally, in the process of described hanging culture; The salinity of water body is lower than 2 ‰; The temperature of water body is 5-30 DEG C; In water body, calcium content is 30-50mg/L, content of magnesium is 0.5-1mg/L, sulfur content is 1-2mg/L, Fe content is 3-5mg/L, iron content is 0.5mg/L, Zn content is 0.05mg/L.
Optionally, in the process of described hanging culture: implant and need described second of transplanted tissue and produce pearl shellfish and be in below water surface 25-35 centimetre.
Optionally, in the process of described hanging culture: the bait in water body comprises: chrysophyceae, diatom, hidden algae.
Embodiment
Below by specific embodiment, the present invention is described in further detail.
Embodiment one
Provide the breeding method of an a kind of pearl polychrome pearl in an embodiment of the present invention, comprise the following steps:
1, multiple first product pearl shellfish with different nacre color is cultivated;
In this step, by directive breeding, can obtain multiple required color first produces pearl shellfish; Concrete color sets according to actual conditions.
Preferably, being built by inbreeding is the breeding group that method sets up different colours respectively, hybridizes between different breeding group again, obtains first and produces pearl shellfish, and this first product pearl shellfish can prevent the degeneration of color trait.
2, gather the cortical tissue on the outer embrane of all described first product pearl shellfishes respectively, obtain multiple tissue;
Because the first cortical tissue produced on the outer embrane of pearl shellfish has the nacreous potential quality of secretion, therefore, gather the epidermal tissue on its outer embrane produce the dissection of pearl shellfish by first after, and then obtain multiple tissue.
In the process gathering tissue, it should be noted that, in order to ensure the activity of tissue, need after acquisition to utilize physiological saline, trypsase and antimicrobial to maintain, therefore, the tissue obtained is the tissue containing a large amount of cell mass, so ensure its after go can be formed smoothly in the process of cell chulture and fusion to treat transplanted tissue.
3, all described tissue (cells containing a large amount of) are merged, obtain treating transplanted tissue;
After obtaining multiple tissue, by the cell culture technology of routine, multiple tissue can be fused into and treat transplanted tissue's (after cultivating and merging, its function class is like pearl sac).
4, treat that transplanted tissue implants in the second pallial connective tissue producing pearl shellfish or internal sac by described, and cultivate, obtain a pearl polychrome pearl.
In above-mentioned steps, by treat transplanted tissue implant second produce pearl shellfish in, in order to ensure the success rate implanted, in the pallial connective tissue needing to be implanted to the second product pearl shellfish or internal sac, and then the second product pearl shellfish is cultivated, in the process of growth, it can produce the nacre of polychrome, after growth certain hour, polychrome pearl can be obtained.
The breeding method of the pearl polychrome pearl that the embodiment of the present invention provides, first it cultivated the first product pearl shellfish with different nacre color, and multiple first produces pearl shellfishes can secrete the nacre producing different colours.So, by gathering the cortical tissue on the outer embrane of all first product pearl shellfishes, obtain multiple tissue, multiple tissue can obtain tissue (similar pearl sac) to be transplanted by cell culture technology again, this tissue possesses the nacreous characteristic of secretion different colours, therefore, is implanted second and produces in the pallial connective tissue of pearl shellfish, and cultivate, a pearl polychrome pearl can be obtained.
Compared with prior art, the breeding method of a this pearl polychrome pearl provided by the invention, it can produce polychrome pearl, the kind of the first product pearl shellfish specifically can be selected according to required color, polychrome pearl rich color, there is higher enjoying value, therefore, can well business demand be met.
This method that the embodiment of the present invention provides thoroughly has overturned eternal law of an a kind of color of pearl, and make pearl sector produce historic shift, a pearl polychrome, changes the original concept of pearl completely, is the revolutionary achievement of pearl research field.The batch production of " a pearl polychrome " pearl, will thoroughly change pearl market to the appraisement system of pearl quality and world's pearl sector general layout.
In order to the breeding method of the pearl polychrome pearl making the embodiment of the present invention one is better applied, more effectively in employing multicolor pearl cultivating, the present invention also provides embodiment two on the basis of above-described embodiment, embodiment two is further restriction to the method in embodiment one and increase, is now described in detail and explains:
Embodiment two
The preparation method that the embodiment of the present invention provides comprises the following steps:
A, cultivate and multiplely there is first of different nacre color produce pearl shellfish;
Concrete breeding method is identical with the step 1 in embodiment one, in the present embodiment, only the kind that first produces pearl shellfish is specifically defined as hydriopsis cumingii (Hyriopsiscumingii).And, preferred, in order to can the colour system of genetic stability, above-mentioned hydriopsis cumingii (Hyriopsis cumingii) be for taking unexpected mass incident, family selective breeding method, through the selection at least 4 generations, the nacreous freshwater mussel offspring of white, gold, redness and pansy can be secreted.
In the present embodiment, hydriopsis cumingii (Hyriopsis cumingii) why is selected to produce pearl shellfish as first, because it possesses the nacreous ability of stronger secretion on the one hand, on the other hand, the color of its nacre possessed can be white, gold, redness and violet stock violet, this several color is compared with other pearls, and it is more subject to liking of consumers in general.
In addition, the selection of the second product pearl shellfish is then comparatively various, as long as the kind of Mollusca, Bivalvia, Eulamellibranchia Margaritanidae, Unionidae or Mollusca Bivalvia Anisomyaria Pteriidae, the present embodiment selects hydriopsis cumingii (Hyriopsis cumingii).
It is pointed out that and produce except pearl shellfish except choosing hydriopsis cumingii as first, pond dish freshwater mussel, cristaria plicata, anodon, beautiful freshwater mussel, pteria martensii, large nacre or Pteria penguin also can produce pearl shellfish as first.
B, the cortical tissue gathered respectively on the outer embrane of all described first product pearl shellfishes, obtain multiple tissue;
In this step, preferably, choose and long for the shell multiple first product pearl shellfishes for 7-10 centimetre are dissected, gather the epidermal tissue on described first product pearl shellfish jacket membrane respectively, and cut into massive texture, have 7-10 centimetre, the physiologically active of its whole shellfish is comparatively stable, vitality is comparatively vigorous, therefore can improve the success rate of collection and subsequent operation.
It is to be noted, in order to ensure the physiologically active of the tissue of gained, and then provide safeguard for producing nacre, after obtaining multiple massive texture, need all massive textures to utilize the mixed liquor containing physiological saline, trypsase and gentamicin to maintain, obtain the tissue containing cell mass.
C, all described tissue to be merged, obtain treating transplanted tissue;
This step is that multiple tissue is treated the process of transplanted tissue by it by cell chulture and then acquisition, in the process of cultivating and merge, all tissues or cell all can be bred and be healed, and then be grown to function class like pearl sac treat transplanted tissue, after the second product pearl shellfish is ready to, then implant procedure can be carried out.
D, treat that transplanted tissue implants the pallial connective tissue that second produces pearl shellfish by described;
In this step, concrete, while treating that transplanted tissue implants the pallial connective tissue of the second product pearl shellfish, also in the pallial connective tissue or internal sac of described second product pearl shellfish, inserting strain core, by cultivating, obtaining a pearl polychrome pearl.
Like this, after cultivating (hanging culture 2-5), the nacre of its secretion can be accumulated in the surface of pearl core and then form polychrome nucleated pearl.
Generally speaking, in the process of hanging culture, through the cultivation of 3-5 year, can obtain a pearl polychrome without nucleus pearl (namely do not implant pearl core, but which produce the pearl time longer, therefore be of little use); Through the cultivation of 2 years, the nucleated pearl of a pearl polychrome can be obtained.
In concrete cultivating process, preferably, needed for the growth conditions that ensures the second product pearl shellfish and nutrition, following condition control can be carried out:
The salinity of water body is lower than 2 ‰ (preferably 2 ‰); The temperature of water body is 5-30 DEG C; In water body, calcium content is 30-50mg/L, content of magnesium is 0.5-1mg/L, sulfur content is 1-2mg/L, Fe content is 3-5mg/L, iron content is 0.5mg/L, Zn content is 0.05mg/L;
In above-mentioned salinity and temperature range, every physical signs of the second product pearl shellfish is all more stable, therefore, is applicable to very much the quick growth that second produces pearl shellfish.
In addition, in the process of cultivating, preferably, also defining the concentration of each mineral salt in water body, is further that the growth of the second product pearl shellfish provides nutrition assurance, growth that can be healthy fast.
It is pointed out that in the process of hanging culture, for the ease of operation, and ensure suitable illumination, implant and need described second of transplanted tissue and produce pearl shellfish and be in below water surface 25-35 centimetre.Like this, on the one hand, be convenient to manual operation, can ensure that the second product pearl shellfish can have sufficient illumination on the other hand.
When concrete setting, also the transparency of water body is limited (25-40 centimetres), so that operator observes the growing state of shellfish at any time.
Because bivalve shellfish is generally all using phytoplankton as bait, by filter food, to supplement its nutrition, in the present embodiment, preferred bait comprises chrysophyceae, diatom, hidden algae; Chrysophyceae, diatom, hidden algae its wide material sources on the one hand, cost is low, and more easily cultivates; On the other hand, above-mentioned food nutrition comparatively horn of plenty, is highly susceptible to being ingested by bivalve shellfish.And chrysophyceae, diatom, hidden algae also contribute to the nacre of the second product pearl shellfish stably excreting given color.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (9)
1. the breeding method of a pearl polychrome pearl, is characterized in that, comprise the following steps:
Cultivate multiple first product pearl shellfish with different nacre color;
Gather the cortical tissue on the outer embrane of all described first product pearl shellfishes respectively, obtain multiple tissue;
All described tissue are merged, obtains treating transplanted tissue;
Treat that transplanted tissue implants in the second pallial connective tissue producing pearl shellfish or internal sac by described, and cultivate, obtain a pearl polychrome pearl.
2. breeding method according to claim 1, is characterized in that, described first produces pearl shellfish comprises: hydriopsis cumingii, pond dish freshwater mussel, cristaria plicata, anodon, beautiful freshwater mussel, large nacre, pteria martensii or Pteria penguin;
Described second produces pearl shellfish comprises: the kind of Mollusca Bivalvia Eulamellibranchia Margaritanidae or Mollusca Bivalvia Anisomyaria Pteriidae.
3. breeding method according to claim 1 and 2, is characterized in that, in the step of the multiple first product pearl shellfish with different nacre color of described cultivation,
Described first product pearl shellfish is at least through the seed selection of 4 generations, and can secrete the nacreous shellfish of pure color.
4. breeding method according to claim 3, is characterized in that, the cortical tissue on the described outer embrane gathering all described first product pearl shellfishes respectively, obtains, in the step of multiple tissue, specifically comprising:
Long for the shell multiple first product pearl shellfishes for 7-10 centimetre are dissected, gathers the cortical tissue on described first product pearl shellfish jacket membrane respectively, and cut into massive texture;
Utilize the mixed liquor containing physiological saline, trypsase and gentamicin to maintain all massive textures, obtain the tissue containing cell mass.
5. breeding method according to claim 4, is characterized in that, treats that transplanted tissue implants in the second pallial connective tissue producing pearl shellfish or internal sac, and cultivates, obtain in the step of a pearl polychrome pearl, specifically comprise described by described:
By described treat that transplanted tissue implants the second pallial connective tissue producing pearl shellfish or internal sac while, also producing in the pallial connective tissue of pearl shellfish or internal sac described second and insert strain core, by cultivating, obtaining a pearl polychrome pearl.
6. breeding method according to claim 5, it is characterized in that, treat that transplanted tissue implants in the second pallial connective tissue producing pearl shellfish or internal sac described by described, and cultivate, obtain in the step of a pearl polychrome pearl, described cultivation specifically comprises:
Need described second of transplanted tissue produce pearl shellfish hanging culture 2-5 in water by implanting.
7. breeding method according to claim 6, is characterized in that, in the process of described hanging culture:
The salinity of water body is lower than 2 ‰; The temperature of water body is 5-30 DEG C;
In water body, calcium content is 30-50mg/L, content of magnesium is 0.5-1mg/L, sulfur content is 1-2mg/L, Fe content is 3-5mg/L, iron content is 0.5mg/L, Zn content is 0.05mg/L.
8. the breeding method according to claim 6 or 7, is characterized in that, in the process of described hanging culture:
Implant and need described second of transplanted tissue and produce pearl shellfish and be in below water surface 25-35 centimetre.
9. breeding method according to claim 8, is characterized in that, in the process of described hanging culture:
Bait in water body comprises: chrysophyceae, diatom, hidden algae.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410122901.8A CN103858804B (en) | 2014-03-28 | 2014-03-28 | The breeding method of one pearl polychrome pearl |
| AU2014321176A AU2014321176B2 (en) | 2014-03-28 | 2014-05-04 | Cultivating method for multicolor pearl |
| JP2016509288A JP6096982B2 (en) | 2014-03-28 | 2014-05-04 | Multi-colored pearl farming method |
| PCT/CN2014/076706 WO2015143755A1 (en) | 2014-03-28 | 2014-05-04 | Method for cultivating multi-color pearl |
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| CN201410122901.8A CN103858804B (en) | 2014-03-28 | 2014-03-28 | The breeding method of one pearl polychrome pearl |
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| CN103858804A CN103858804A (en) | 2014-06-18 |
| CN103858804B true CN103858804B (en) | 2015-09-02 |
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| JP (1) | JP6096982B2 (en) |
| CN (1) | CN103858804B (en) |
| AU (1) | AU2014321176B2 (en) |
| WO (1) | WO2015143755A1 (en) |
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| CN111528148A (en) * | 2020-05-30 | 2020-08-14 | 抚州市水产科学研究所 | Method for rapidly cultivating high-quality nucleated pearls by using hyriopsis schlegeli |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06245667A (en) * | 1993-02-24 | 1994-09-06 | Yoshinaga Nakai | Nucleus for culturing pearl and its production |
| CN1351825A (en) * | 2001-12-25 | 2002-06-05 | 胡耀华 | Indoor stereo fast high efficiency high quality pearl inoculation technology |
| FR2835700B1 (en) * | 2002-02-13 | 2006-04-07 | Robert Wan Holding | PROCESS FOR CULTIVATION OF PEARLS |
| JP2004024062A (en) * | 2002-06-24 | 2004-01-29 | Matsushita Pearl Co Ltd | Pearl breeding method |
| CN1416694A (en) * | 2002-11-13 | 2003-05-14 | 石子聪 | Color pearl growing method |
| US7404378B2 (en) * | 2006-02-17 | 2008-07-29 | Batzer William B | Pearl culture method and product |
| CN103210860B (en) * | 2013-04-08 | 2015-02-04 | 诸暨博师珍珠业生产力促进中心有限公司 | Method for culturing fresh water colored pearls |
| CN103478028B (en) * | 2013-09-03 | 2015-02-25 | 金华职业技术学院 | Directional cultivation method of white or purple fresh water pearl |
| CN103598129B (en) * | 2013-12-02 | 2015-10-07 | 浙江海洋学院 | The breeding method of iris fresh water pearl |
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2014
- 2014-03-28 CN CN201410122901.8A patent/CN103858804B/en active Active
- 2014-05-04 WO PCT/CN2014/076706 patent/WO2015143755A1/en active Application Filing
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| CN103858804A (en) | 2014-06-18 |
| WO2015143755A1 (en) | 2015-10-01 |
| AU2014321176B2 (en) | 2015-12-10 |
| JP2016519578A (en) | 2016-07-07 |
| AU2014321176A1 (en) | 2015-10-15 |
| JP6096982B2 (en) | 2017-03-15 |
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