CN103858804A - Method for cultivating multi-color pearl - Google Patents
Method for cultivating multi-color pearl Download PDFInfo
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- CN103858804A CN103858804A CN201410122901.8A CN201410122901A CN103858804A CN 103858804 A CN103858804 A CN 103858804A CN 201410122901 A CN201410122901 A CN 201410122901A CN 103858804 A CN103858804 A CN 103858804A
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- 238000000034 method Methods 0.000 title claims abstract description 29
- 235000015170 shellfish Nutrition 0.000 claims abstract description 84
- 210000001519 tissue Anatomy 0.000 claims abstract description 67
- 210000002808 connective tissue Anatomy 0.000 claims abstract description 19
- 239000003086 colorant Substances 0.000 claims abstract description 17
- PLXMOAALOJOTIY-FPTXNFDTSA-N Aesculin Natural products OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)[C@H]1Oc2cc3C=CC(=O)Oc3cc2O PLXMOAALOJOTIY-FPTXNFDTSA-N 0.000 claims description 36
- 238000009395 breeding Methods 0.000 claims description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 239000007943 implant Substances 0.000 claims description 17
- 230000008569 process Effects 0.000 claims description 15
- 210000005257 cortical tissue Anatomy 0.000 claims description 9
- 241000237536 Mytilus edulis Species 0.000 claims description 7
- 239000013505 freshwater Substances 0.000 claims description 7
- 235000020638 mussel Nutrition 0.000 claims description 7
- 241000237519 Bivalvia Species 0.000 claims description 6
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 6
- 241000206751 Chrysophyceae Species 0.000 claims description 5
- 241000195493 Cryptophyta Species 0.000 claims description 5
- 239000002504 physiological saline solution Substances 0.000 claims description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 3
- 241000382825 Cristaria plicata Species 0.000 claims description 3
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 claims description 3
- 229930182566 Gentamicin Natural products 0.000 claims description 3
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 3
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims description 3
- 241000879903 Pteria Species 0.000 claims description 3
- 241000183300 Pteria penguin Species 0.000 claims description 3
- 241001464055 Pteriidae Species 0.000 claims description 3
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 3
- 241000217379 Unionidae Species 0.000 claims description 3
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 3
- 239000011575 calcium Substances 0.000 claims description 3
- 229910052791 calcium Inorganic materials 0.000 claims description 3
- 229960002518 gentamicin Drugs 0.000 claims description 3
- 238000002513 implantation Methods 0.000 claims description 3
- 229910052742 iron Inorganic materials 0.000 claims description 3
- 239000011777 magnesium Substances 0.000 claims description 3
- 229910052749 magnesium Inorganic materials 0.000 claims description 3
- 229910052748 manganese Inorganic materials 0.000 claims description 3
- 239000011572 manganese Substances 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 239000011593 sulfur Substances 0.000 claims description 3
- 229910052717 sulfur Inorganic materials 0.000 claims description 3
- 239000011701 zinc Substances 0.000 claims description 3
- 229910052725 zinc Inorganic materials 0.000 claims description 3
- 230000008859 change Effects 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 238000011156 evaluation Methods 0.000 abstract 1
- 210000001835 viscera Anatomy 0.000 abstract 1
- 239000011049 pearl Substances 0.000 description 142
- 239000000047 product Substances 0.000 description 27
- 230000028327 secretion Effects 0.000 description 6
- 241000382840 Hyriopsis cumingii Species 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 230000035764 nutrition Effects 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
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- 241000237852 Mollusca Species 0.000 description 1
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- 235000013305 food Nutrition 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009399 inbreeding Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to the field of pearl cultivating, in particular to a method for cultivating a multi-color pearl. The method comprises the following steps that a plurality of first pearl producing shellfishes with different pearl layer colors are cultivated; skin tissues on the mantles of the first pearl producing shellfishes are respectively collected to obtain a plurality of tissue sheets; all the tissue sheets are combined to obtain a tissue to obtain a tissue to be transplanted; the tissue to be transplanted is transplanted into the connective tissue or the viscera sac of the mantel of a second pearl protruding shellfish to be cultivated, and then the multi-color pearl is obtained. The method thoroughly overturns the eternal rule that one pearl has one color, the pearl industry is historically changed, one pearl has multiple colors, the original concept on the pearl is completely changed, and the multi-color pearl is the revolutionary achievement in the pearl studying field. The batched production of the multi-color pearl will thoroughly change an evaluation system of the pearl industry to the pearl quality and the world pearl industrial pattern.
Description
Technical field
The present invention relates to pearl and cultivate field, in particular to the breeding method of an a kind of pearl polychrome pearl.
Background technology
The most important condition that pearl forms is to generate and have to internal secretion nacreous " pearl sac " at product pearl shellfish soft tissue position (connective tissue etc.); Traditional free pearl production method will be traced back to Japanese before more than 100 year and see the mantle tissue patch-graft method of rapids occasion equality, and this method generates the technology of pearl sac after the whole world is universal, becomes the basic technology that adopts modern artificial pearl culture so far.
But in correlation technique, the pearl homogeneous that traditional pearl cultivating method produces only possesses a kind of color (as white, gold, redness, yellow or purple etc.); Because its color is single, tone is simple; Therefore, can not meet business demand.
Summary of the invention
The object of the present invention is to provide the breeding method of an a kind of pearl polychrome pearl, to solve the above problems.
The breeding method that an a kind of pearl polychrome pearl is provided in an embodiment of the present invention, comprises the following steps:
Cultivate multiple first product pearl shellfishes with different nacre colors;
Gather respectively all described first cortical tissue producing on the outer embranes of pearl shellfishes, obtain multiple tissue;
All described tissue are merged, obtain treating transplanted tissue;
Treat that by described transplanted tissue implants second and produces in the pallial connective tissue or internal sac of pearl shellfish, and cultivate, obtain a pearl polychrome pearl.
The breeding method of the pearl polychrome pearl that the embodiment of the present invention provides, first it cultivated the first product pearl shellfish with different nacre colors, and multiple first produces pearl shellfishes can secrete the nacre that produces different colours; So, by gathering all first cortical tissue producing on the outer embranes of pearl shellfishes, obtain multiple tissue, multiple tissue are cultivated and can be obtained tissue to be transplanted (its similar pearl sac) by cell again, this tissue possesses the nacreous characteristic of secretion different colours, therefore, is implanted second and produces in the pallial connective tissue or internal sac of pearl shellfish, and cultivate, can obtain a pearl polychrome pearl.
Compared with prior art, the breeding method of a this pearl polychrome pearl provided by the invention, it can produce polychrome pearl, can specifically select the kind of the first product pearl shellfish according to required color, polychrome pearl rich color, there is higher enjoying value, therefore, can well meet business demand.
This method that the embodiment of the present invention provides has thoroughly been overturned eternal law of an a kind of color of pearl, makes pearl sector produce historic shift, and a pearl polychrome, has changed the original concept of pearl completely, is the revolutionary achievement of pearl research field.The batch production of " a pearl polychrome " pearl, will thoroughly change appraisement system and the world pearl sector general layout of pearl market to pearl quality.
Optionally, described first produce pearl shellfish and comprise: hydriopsis cumingii, pond dish freshwater mussel, cristaria plicata, anodon, beautiful freshwater mussel, large nacre, pteria martensii or Pteria penguin.
Described second produces pearl shellfish comprises: described second produces pearl shellfish comprises: the kind of Mollusca Bivalvia Eulamellibranchia Margaritanidae, Unionidae or Mollusca Bivalvia Anisomyaria Pteriidae.
Optionally, in the step of the multiple first product pearl shellfishes with different nacre colors of described cultivation,
Described first produce pearl shellfish at least through the seed selection of 4 generations, and can secrete the nacreous shellfish of pure color.
Optionally, cortical tissue on the described outer embrane that gathers respectively all described the first product pearl shellfishes, obtain in the step of multiple tissue, specifically comprise: long shell multiple the first product pearl shellfishes for 7-10 centimetre are dissected, gather respectively the described first epidermal tissue producing on pearl shellfish jacket membrane, and cut into massive texture; The mixed liquor that all massive texture utilizations are contained to physiological saline, trypsase and gentamicin maintains, and obtains the tissue that contains cell mass.
Optionally, treat that by described transplanted tissue implants second and produces in the pallial connective tissue or internal sac of pearl shellfish described, and cultivate, obtain in the step of a pearl polychrome pearl, specifically comprise: treat that by described transplanted tissue implants second when producing the pallial connective tissue of pearl shellfish or internal sac, also in described second produces the pallial connective tissue of pearl shellfish or internal sac, insert strain core, by cultivating, obtain a pearl polychrome pearl.
Optionally, treat that by described transplanted tissue implants second and produces in the pallial connective tissue or internal sac of pearl shellfish described, and cultivate, obtain in the step of a pearl polychrome pearl, described cultivation specifically comprises: implantation is needed to described second of transplanted tissue and produce pearl shellfish hanging culture 2-5 in water.
Optionally, in the process of described hanging culture; The salinity of water body is lower than 2 ‰; The temperature of water body is 5-30 DEG C; In water body, calcium content is that 30-50mg/L, content of magnesium are that 0.5-1mg/L, sulfur content are that 1-2mg/L, manganese content are that 3-5mg/L, iron content are that 0.5mg/L, zinc content are 0.05mg/L.
Optionally, in the process of described hanging culture: implant and need described second of transplanted tissue and produce pearl shellfish in the following 25-35 centimetre of the water surface.
Optionally, in the process of described hanging culture: the bait in water body comprises: chrysophyceae, diatom, hidden algae.
Embodiment
Below by specific embodiment, the present invention is described in further detail.
Embodiment mono-
The breeding method that an a kind of pearl polychrome pearl is provided in an embodiment of the present invention, comprises the following steps:
1, cultivate multiple first product pearl shellfishes with different nacre colors;
In this step, by directive breeding, can obtain first of multiple required colors and produce pearl shellfish; Concrete color is set according to actual conditions.
Preferably, building by inbreeding is the breeding group that method is set up respectively different colours, between different breeding groups, hybridizes again, obtains first and produces pearl shellfish, and this first product pearl shellfish can prevent the degeneration of color proterties.
2, gather respectively all described first cortical tissue producing on the outer embranes of pearl shellfishes, obtain multiple tissue;
Because the cortical tissue on the outer embrane of the first product pearl shellfish has the nacreous potential quality of secretion, therefore, after the first product pearl shellfish is dissected, gather the epidermal tissue on its outer embrane, and then obtain multiple tissue.
In the process of collection tissue, it should be noted that, in order to ensure the activity of tissue, after collection, need to utilize physiological saline, trypsase and antimicrobial to maintain, therefore, the tissue obtaining is the tissue that contains a large amount of cell masses, so ensure its after go cell cultivate and the process that merges in can form smoothly and treat transplanted tissue.
3, all described tissue (containing a large amount of cells) are merged, obtain treating transplanted tissue;
After obtaining multiple tissue, by conventional cell culture technology, multiple tissue can be fused into and treat transplanted tissue's (by after cultivating and merging, its function class is like pearl sac).
4, treat that by described transplanted tissue implants second and produces in the pallial connective tissue or internal sac of pearl shellfish, and cultivate, obtain a pearl polychrome pearl.
In above-mentioned steps, by treat transplanted tissue implant second produce pearl shellfish in, for the success rate that ensures to implant, need to be implanted in the pallial connective tissue or internal sac of the second product pearl shellfish, and then the second product pearl shellfish is cultivated, in the process of growth, it can produce the nacre of polychrome, after growth certain hour, can obtain polychrome pearl.
The breeding method of the pearl polychrome pearl that the embodiment of the present invention provides, first it cultivated the first product pearl shellfish with different nacre colors, and multiple first produces pearl shellfishes can secrete the nacre that produces different colours.So, by gathering all first cortical tissue producing on the outer embranes of pearl shellfishes, obtain multiple tissue, multiple tissue can obtain tissue to be transplanted (similar pearl sac) by cell culture technology again, this tissue possesses the nacreous characteristic of secretion different colours, therefore, is implanted second and produces in the pallial connective tissue of pearl shellfish, and cultivate, can obtain a pearl polychrome pearl.
Compared with prior art, the breeding method of a this pearl polychrome pearl provided by the invention, it can produce polychrome pearl, can specifically select the kind of the first product pearl shellfish according to required color, polychrome pearl rich color, there is higher enjoying value, therefore, can well meet business demand.
This method that the embodiment of the present invention provides has thoroughly been overturned eternal law of an a kind of color of pearl, makes pearl sector produce historic shift, and a pearl polychrome, has changed the original concept of pearl completely, is the revolutionary achievement of pearl research field.The batch production of " a pearl polychrome " pearl, will thoroughly change appraisement system and the world pearl sector general layout of pearl market to pearl quality.
For the breeding method of the pearl polychrome pearl that makes the embodiment of the present invention one is better applied, more effectively in employing multicolor pearl cultivating, the present invention also provides embodiment bis-on the basis of above-described embodiment, embodiment bis-is further restriction and the increases to the method in embodiment mono-, is now described in detail and explains:
Embodiment bis-
The preparation method that the embodiment of the present invention provides comprises the following steps:
A, cultivate and multiplely there are first of different nacre colors and produce pearl shellfishes;
Concrete breeding method is identical with the step 1 in embodiment mono-, in the present embodiment, only the kind of the first product pearl shellfish is specifically defined as to hydriopsis cumingii (Hyriopsis cumingii).And, preferred, for colour system that can genetic stability, above-mentioned hydriopsis cumingii (Hyriopsis cumingii) is for taking colony's seed selection, family selective breeding method, through the selection at least 4 generations, can secrete the nacreous freshwater mussel offspring of white, gold, redness and pansy.
In the present embodiment, why select hydriopsis cumingii (Hyriopsis cumingii) as the first product pearl shellfish, because it possesses the nacreous ability of stronger secretion on the one hand, on the other hand, the color of its nacre possessing can be white, gold, redness and violet stock violet, these several colors are compared with other pearls, and it is more subject to liking of consumers in general.
In addition, the selection of the second product pearl shellfish is comparatively various, as long as the kind of Mollusca, Bivalvia, Eulamellibranchia Margaritanidae, Unionidae or Mollusca Bivalvia Anisomyaria Pteriidae, the present embodiment is selected hydriopsis cumingii (Hyriopsis cumingii).
It is pointed out that pond dish freshwater mussel, cristaria plicata, anodon, beautiful freshwater mussel, pteria martensii, large nacre or Pteria penguin also can be served as the first product pearl shellfish except choosing hydriopsis cumingii as the first product pearl shellfish.
B, gather all described first cortical tissue producing on the outer embranes of pearl shellfishes respectively, obtain multiple tissue;
In this step, preferably, choose long shell multiple the first product pearl shellfishes for 7-10 centimetre are dissected, gather respectively the described first epidermal tissue producing on pearl shellfish jacket membrane, and cut into massive texture, and thering is 7-10 centimetre, the physiologically active of its whole shellfish is comparatively stable, vitality is comparatively vigorous, therefore can improve the success rate of collection and subsequent operation.
It is to be noted, in order to ensure the physiologically active of tissue of gained, and then provide safeguard for producing nacre, after obtaining multiple massive textures, the mixed liquor that all massive texture utilizations need to be contained to physiological saline, trypsase and gentamicin maintains, and obtains the tissue that contains cell mass.
C, all described tissue are merged, obtain treating transplanted tissue;
This step is that it cultivates multiple tissue and then obtain by cell the process for the treatment of transplanted tissue, in the process of cultivating and merge, all tissues or cell all can be bred and heal, and then be grown to function class and treat transplanted tissue like pearl sac, after the second product pearl shellfish is ready to, can carry out implant procedure.
D, treat that by described transplanted tissue implants second and produces the pallial connective tissue of pearl shellfish;
In this step, concrete, will treat that transplanted tissue implants second when producing the pallial connective tissue of pearl shellfish, also in described second produces the pallial connective tissue of pearl shellfish or internal sac, insert strain core, by cultivating, obtain a pearl polychrome pearl.
Like this, by cultivating (hanging culture 2-5) afterwards, the nacre of its secretion can be accumulated in the surface of pearl core and then form polychrome nucleated pearl.
Generally speaking, in the process of hanging culture, through the cultivation of 3-5 year, can obtain a pearl polychrome without nucleus pearl (do not implant pearl core, but which produce the pearl time longer, therefore be of little use); Through the cultivation of 2 years, can obtain the nucleated pearl of a pearl polychrome.
In concrete cultivating process, preferred, required in order to ensure growth conditions and the nutrition of the second product pearl shellfish, can carry out following condition control:
The salinity of water body is lower than 2 ‰ (preferably 2 ‰); The temperature of water body is 5-30 DEG C; In water body, calcium content is that 30-50mg/L, content of magnesium are that 0.5-1mg/L, sulfur content are that 1-2mg/L, manganese content are that 3-5mg/L, iron content are that 0.5mg/L, zinc content are 0.05mg/L;
In above-mentioned salinity and temperature range, every physical signs of the second product pearl shellfish is all more stable, therefore, is applicable to very much second and produces the Fast Growth of pearl shellfish.
In addition, in the process of cultivating, preferably, also define the concentration of each mineral salt in water body, further for the growth of the second product pearl shellfish provides nutrition guarantee, growth that can be healthy fast.
It is pointed out that in the process of hanging culture, for the ease of operation, and ensure suitable illumination, implant and need described second of transplanted tissue and produce pearl shellfish in the following 25-35 centimetre of the water surface.Like this, on the one hand, be convenient to manual operation, can ensure that on the other hand the second product pearl shellfish can have sufficient illumination.
In concrete setting, also the transparency of water body is limited to (25-40 centimetres), so that operator observes the growing state of shellfish at any time.
Eat by filter all using phytoplankton as bait because bivalve shellfish is general, to supplement its nutrition, in the present embodiment, preferred bait comprises chrysophyceae, diatom, hidden algae; Chrysophyceae, diatom, hidden algae its wide material sources on the one hand, cost is low, and more easily cultivates; On the other hand, above-mentioned food nutrition, compared with horn of plenty, is highly susceptible to being ingested by bivalve shellfish.And chrysophyceae, diatom, hidden algae also contribute to the nacre of the set color of the second product pearl shellfish stably excreting.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (9)
1. the breeding method of a pearl polychrome pearl, is characterized in that, comprises the following steps:
Cultivate multiple first product pearl shellfishes with different nacre colors;
Gather respectively all described first cortical tissue producing on the outer embranes of pearl shellfishes, obtain multiple tissue;
All described tissue are merged, obtain treating transplanted tissue;
Treat that by described transplanted tissue implants second and produces in the pallial connective tissue or internal sac of pearl shellfish, and cultivate, obtain a pearl polychrome pearl.
2. breeding method according to claim 1, is characterized in that, described first produces pearl shellfish comprises: hydriopsis cumingii, pond dish freshwater mussel, cristaria plicata, anodon, beautiful freshwater mussel, large nacre, pteria martensii or Pteria penguin;
Described second produces pearl shellfish comprises: the kind of Mollusca Bivalvia Eulamellibranchia Margaritanidae, Unionidae or Mollusca Bivalvia Anisomyaria Pteriidae.
3. breeding method according to claim 1 and 2, is characterized in that, in the step of the multiple first product pearl shellfishes with different nacre colors of described cultivation,
Described first produce pearl shellfish at least through the seed selection of 4 generations, and can secrete the nacreous shellfish of pure color.
4. breeding method according to claim 3, is characterized in that, the cortical tissue on the described outer embrane that gathers respectively all described the first product pearl shellfishes, obtains, in the step of multiple tissue, specifically comprising:
Long shell multiple the first product pearl shellfishes for 7-10 centimetre are dissected, gathered respectively the described first epidermal tissue producing on pearl shellfish jacket membrane, and cut into massive texture;
The mixed liquor that all massive texture utilizations are contained to physiological saline, trypsase and gentamicin maintains, and obtains the tissue that contains cell mass.
5. breeding method according to claim 4, is characterized in that, treats that transplanted tissue implants second and produces in the pallial connective tissue or internal sac of pearl shellfish, and cultivate described by described, obtains in the step of a pearl polychrome pearl, specifically comprises:
Treat that by described transplanted tissue implants second when producing the pallial connective tissue of pearl shellfish or internal sac, also in described second produces the pallial connective tissue of pearl shellfish or internal sac, insert strain core, by cultivating, obtain a pearl polychrome pearl.
6. breeding method according to claim 5, it is characterized in that, treat that by described transplanted tissue implants second and produces in the pallial connective tissue or internal sac of pearl shellfish described, and cultivate, obtain in the step of a pearl polychrome pearl, described cultivation specifically comprises:
Implantation is needed to described second of transplanted tissue and produce pearl shellfish hanging culture 2-5 in water.
7. breeding method according to claim 6, is characterized in that, in the process of described hanging culture;
The salinity of water body is lower than 2 ‰; The temperature of water body is 5-30 DEG C;
In water body, calcium content is that 30-50mg/L, content of magnesium are that 0.5-1mg/L, sulfur content are that 1-2mg/L, manganese content are that 3-5mg/L, iron content are that 0.5mg/L, zinc content are 0.05mg/L.
8. according to the breeding method described in claim 6 or 7, it is characterized in that, in the process of described hanging culture:
Implantation needs described second of transplanted tissue and produces pearl shellfish in the following 25-35 centimetre of the water surface.
9. breeding method according to claim 8, is characterized in that, in the process of described hanging culture:
Bait in water body comprises: chrysophyceae, diatom, hidden algae.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410122901.8A CN103858804B (en) | 2014-03-28 | 2014-03-28 | The breeding method of one pearl polychrome pearl |
| PCT/CN2014/076706 WO2015143755A1 (en) | 2014-03-28 | 2014-05-04 | Method for cultivating multi-color pearl |
| AU2014321176A AU2014321176B2 (en) | 2014-03-28 | 2014-05-04 | Cultivating method for multicolor pearl |
| JP2016509288A JP6096982B2 (en) | 2014-03-28 | 2014-05-04 | Multi-colored pearl farming method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410122901.8A CN103858804B (en) | 2014-03-28 | 2014-03-28 | The breeding method of one pearl polychrome pearl |
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| Publication Number | Publication Date |
|---|---|
| CN103858804A true CN103858804A (en) | 2014-06-18 |
| CN103858804B CN103858804B (en) | 2015-09-02 |
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| CN201410122901.8A Active CN103858804B (en) | 2014-03-28 | 2014-03-28 | The breeding method of one pearl polychrome pearl |
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| Country | Link |
|---|---|
| JP (1) | JP6096982B2 (en) |
| CN (1) | CN103858804B (en) |
| AU (1) | AU2014321176B2 (en) |
| WO (1) | WO2015143755A1 (en) |
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Also Published As
| Publication number | Publication date |
|---|---|
| JP6096982B2 (en) | 2017-03-15 |
| AU2014321176B2 (en) | 2015-12-10 |
| WO2015143755A1 (en) | 2015-10-01 |
| JP2016519578A (en) | 2016-07-07 |
| CN103858804B (en) | 2015-09-02 |
| AU2014321176A1 (en) | 2015-10-15 |
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