CN103849600A - 一种适用于培养nk细胞的无血清培养基 - Google Patents
一种适用于培养nk细胞的无血清培养基 Download PDFInfo
- Publication number
- CN103849600A CN103849600A CN201410118900.6A CN201410118900A CN103849600A CN 103849600 A CN103849600 A CN 103849600A CN 201410118900 A CN201410118900 A CN 201410118900A CN 103849600 A CN103849600 A CN 103849600A
- Authority
- CN
- China
- Prior art keywords
- cell
- component
- serum
- hydrochloride
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000012258 culturing Methods 0.000 title abstract description 6
- 239000004017 serum-free culture medium Substances 0.000 title abstract 5
- 210000000822 natural killer cell Anatomy 0.000 claims abstract description 72
- 239000000306 component Substances 0.000 claims abstract description 20
- 101000623895 Bos taurus Mucin-15 Proteins 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000012679 serum free medium Substances 0.000 claims description 23
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 8
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 claims description 8
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 claims description 8
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 6
- 239000012533 medium component Substances 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- ZUFQODAHGAHPFQ-UHFFFAOYSA-N pyridoxine hydrochloride Chemical compound Cl.CC1=NC=C(CO)C(CO)=C1O ZUFQODAHGAHPFQ-UHFFFAOYSA-N 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 6
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 claims description 6
- 101000917858 Homo sapiens Low affinity immunoglobulin gamma Fc region receptor III-A Proteins 0.000 claims description 5
- 101000917839 Homo sapiens Low affinity immunoglobulin gamma Fc region receptor III-B Proteins 0.000 claims description 5
- 102100029185 Low affinity immunoglobulin gamma Fc region receptor III-B Human genes 0.000 claims description 5
- 108010025020 Nerve Growth Factor Proteins 0.000 claims description 5
- 102000015336 Nerve Growth Factor Human genes 0.000 claims description 5
- 230000010261 cell growth Effects 0.000 claims description 5
- 239000001913 cellulose Substances 0.000 claims description 5
- 229920002678 cellulose Polymers 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 210000002394 ovarian follicle Anatomy 0.000 claims description 5
- 230000004936 stimulating effect Effects 0.000 claims description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 4
- QZNNVYOVQUKYSC-JEDNCBNOSA-N (2s)-2-amino-3-(1h-imidazol-5-yl)propanoic acid;hydron;chloride Chemical compound Cl.OC(=O)[C@@H](N)CC1=CN=CN1 QZNNVYOVQUKYSC-JEDNCBNOSA-N 0.000 claims description 4
- AGBQKNBQESQNJD-SSDOTTSWSA-N (R)-lipoic acid Chemical compound OC(=O)CCCC[C@@H]1CCSS1 AGBQKNBQESQNJD-SSDOTTSWSA-N 0.000 claims description 4
- KWTQSFXGGICVPE-UHFFFAOYSA-N 2-amino-5-(diaminomethylideneamino)pentanoic acid;hydron;chloride Chemical compound Cl.OC(=O)C(N)CCCN=C(N)N KWTQSFXGGICVPE-UHFFFAOYSA-N 0.000 claims description 4
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 claims description 4
- ODHCTXKNWHHXJC-VKHMYHEASA-N 5-oxo-L-proline Chemical compound OC(=O)[C@@H]1CCC(=O)N1 ODHCTXKNWHHXJC-VKHMYHEASA-N 0.000 claims description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 4
- 235000019743 Choline chloride Nutrition 0.000 claims description 4
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 239000004471 Glycine Substances 0.000 claims description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 4
- FFEARJCKVFRZRR-UHFFFAOYSA-N L-Methionine Natural products CSCCC(N)C(O)=O FFEARJCKVFRZRR-UHFFFAOYSA-N 0.000 claims description 4
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims description 4
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims description 4
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 claims description 4
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims description 4
- BVHLGVCQOALMSV-JEDNCBNOSA-N L-lysine hydrochloride Chemical compound Cl.NCCCC[C@H](N)C(O)=O BVHLGVCQOALMSV-JEDNCBNOSA-N 0.000 claims description 4
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims description 4
- 229930195722 L-methionine Natural products 0.000 claims description 4
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 claims description 4
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims description 4
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 claims description 4
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims description 4
- 239000004473 Threonine Substances 0.000 claims description 4
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 claims description 4
- 229930003756 Vitamin B7 Natural products 0.000 claims description 4
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 4
- AGBQKNBQESQNJD-UHFFFAOYSA-N alpha-Lipoic acid Natural products OC(=O)CCCCC1CCSS1 AGBQKNBQESQNJD-UHFFFAOYSA-N 0.000 claims description 4
- 229960001230 asparagine Drugs 0.000 claims description 4
- 235000003704 aspartic acid Nutrition 0.000 claims description 4
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims description 4
- XXWCODXIQWIHQN-UHFFFAOYSA-N butane-1,4-diamine;hydron;dichloride Chemical compound Cl.Cl.NCCCCN XXWCODXIQWIHQN-UHFFFAOYSA-N 0.000 claims description 4
- 239000001110 calcium chloride Substances 0.000 claims description 4
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 4
- 229960003178 choline chloride Drugs 0.000 claims description 4
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 claims description 4
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 4
- 229910000397 disodium phosphate Inorganic materials 0.000 claims description 4
- 235000019800 disodium phosphate Nutrition 0.000 claims description 4
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 claims description 4
- 229960004232 linoleic acid Drugs 0.000 claims description 4
- 235000019136 lipoic acid Nutrition 0.000 claims description 4
- 229960004452 methionine Drugs 0.000 claims description 4
- 235000019171 pyridoxine hydrochloride Nutrition 0.000 claims description 4
- 239000011764 pyridoxine hydrochloride Substances 0.000 claims description 4
- 229960004172 pyridoxine hydrochloride Drugs 0.000 claims description 4
- 229960002477 riboflavin Drugs 0.000 claims description 4
- 235000019192 riboflavin Nutrition 0.000 claims description 4
- 239000002151 riboflavin Substances 0.000 claims description 4
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 claims description 4
- 239000011734 sodium Substances 0.000 claims description 4
- 229910052708 sodium Inorganic materials 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 4
- 229960000344 thiamine hydrochloride Drugs 0.000 claims description 4
- 235000019190 thiamine hydrochloride Nutrition 0.000 claims description 4
- 239000011747 thiamine hydrochloride Substances 0.000 claims description 4
- 229960002663 thioctic acid Drugs 0.000 claims description 4
- 229960002898 threonine Drugs 0.000 claims description 4
- 239000004474 valine Substances 0.000 claims description 4
- 235000011912 vitamin B7 Nutrition 0.000 claims description 4
- 239000011735 vitamin B7 Substances 0.000 claims description 4
- 239000003102 growth factor Substances 0.000 claims description 3
- 238000005374 membrane filtration Methods 0.000 claims description 3
- 229940053128 nerve growth factor Drugs 0.000 claims description 3
- 229910052757 nitrogen Inorganic materials 0.000 claims description 3
- LXNHXLLTXMVWPM-UHFFFAOYSA-N Vitamin B6 Natural products CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 claims description 2
- 230000012010 growth Effects 0.000 claims description 2
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 claims description 2
- 235000019158 vitamin B6 Nutrition 0.000 claims description 2
- 239000011726 vitamin B6 Substances 0.000 claims description 2
- 229940011671 vitamin b6 Drugs 0.000 claims description 2
- 210000004475 gamma-delta t lymphocyte Anatomy 0.000 claims 1
- 210000004027 cell Anatomy 0.000 abstract description 24
- 210000002966 serum Anatomy 0.000 abstract description 23
- 238000004113 cell culture Methods 0.000 abstract description 18
- 239000001963 growth medium Substances 0.000 abstract description 9
- 238000002659 cell therapy Methods 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 abstract description 3
- 230000002147 killing effect Effects 0.000 abstract description 3
- 239000004615 ingredient Substances 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 12
- 230000000694 effects Effects 0.000 description 11
- 239000000203 mixture Substances 0.000 description 8
- 206010028980 Neoplasm Diseases 0.000 description 6
- 239000000463 material Substances 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- 230000004663 cell proliferation Effects 0.000 description 4
- 238000004043 dyeing Methods 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- PFNFFQXMRSDOHW-UHFFFAOYSA-N spermine Chemical compound NCCCNCCCCNCCCN PFNFFQXMRSDOHW-UHFFFAOYSA-N 0.000 description 3
- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 description 2
- MUMGGOZAMZWBJJ-DYKIIFRCSA-N Testostosterone Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 MUMGGOZAMZWBJJ-DYKIIFRCSA-N 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 210000005087 mononuclear cell Anatomy 0.000 description 2
- 210000001915 nurse cell Anatomy 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 2
- ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 description 2
- 229940063675 spermine Drugs 0.000 description 2
- 210000004881 tumor cell Anatomy 0.000 description 2
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 description 1
- 108010001857 Cell Surface Receptors Proteins 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 108010039471 Fas Ligand Protein Proteins 0.000 description 1
- 108050007372 Fibroblast Growth Factor Proteins 0.000 description 1
- 102000018233 Fibroblast Growth Factor Human genes 0.000 description 1
- 102000001398 Granzyme Human genes 0.000 description 1
- 108060005986 Granzyme Proteins 0.000 description 1
- 101100369992 Homo sapiens TNFSF10 gene Proteins 0.000 description 1
- 101000611183 Homo sapiens Tumor necrosis factor Proteins 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- 102100037209 Peroxisomal N(1)-acetyl-spermine/spermidine oxidase Human genes 0.000 description 1
- 102000013275 Somatomedins Human genes 0.000 description 1
- 108700012411 TNFSF10 Proteins 0.000 description 1
- 102100040247 Tumor necrosis factor Human genes 0.000 description 1
- 102100024598 Tumor necrosis factor ligand superfamily member 10 Human genes 0.000 description 1
- 102100031988 Tumor necrosis factor ligand superfamily member 6 Human genes 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000003470 adrenal cortex hormone Substances 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000002924 anti-infective effect Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 210000001772 blood platelet Anatomy 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 231100000433 cytotoxic Toxicity 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 229960003957 dexamethasone Drugs 0.000 description 1
- UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000012636 effector Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229930182833 estradiol Natural products 0.000 description 1
- 229960005309 estradiol Drugs 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 229940126864 fibroblast growth factor Drugs 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229960000890 hydrocortisone Drugs 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- -1 inorganics Chemical class 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 102000006240 membrane receptors Human genes 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 229920000768 polyamine Polymers 0.000 description 1
- 108010089000 polyamine oxidase Proteins 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000000186 progesterone Substances 0.000 description 1
- 229960003387 progesterone Drugs 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 229940063673 spermidine Drugs 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000003270 steroid hormone Substances 0.000 description 1
- 229960003604 testosterone Drugs 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
培养基类型 | 培养20天NK细胞百分比(%) |
M1 | 85 |
M2 | 47 |
M3 | 35 |
M4 | 27 |
Claims (4)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410118900.6A CN103849600A (zh) | 2014-03-27 | 2014-03-27 | 一种适用于培养nk细胞的无血清培养基 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410118900.6A CN103849600A (zh) | 2014-03-27 | 2014-03-27 | 一种适用于培养nk细胞的无血清培养基 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN103849600A true CN103849600A (zh) | 2014-06-11 |
Family
ID=50857725
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410118900.6A Pending CN103849600A (zh) | 2014-03-27 | 2014-03-27 | 一种适用于培养nk细胞的无血清培养基 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN103849600A (zh) |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104152412A (zh) * | 2014-08-13 | 2014-11-19 | 赛业(苏州)生物科技有限公司 | 一种适用于临床的高效扩增nk细胞的培养体系 |
CN104152413A (zh) * | 2014-08-13 | 2014-11-19 | 赛业(苏州)生物科技有限公司 | 一种适用于临床的高效扩增nk细胞的培养体系的应用 |
CN104450614A (zh) * | 2014-12-05 | 2015-03-25 | 上海安集协康生物技术有限公司 | 一种无动物蛋白源的免疫细胞无血清培养基及其使用方法 |
CN104593324A (zh) * | 2014-11-28 | 2015-05-06 | 广州赛莱拉干细胞科技股份有限公司 | 一种自然杀伤细胞的培养基及自然杀伤细胞的扩增培养方法 |
CN104818248A (zh) * | 2015-03-25 | 2015-08-05 | 苏州佰通生物科技有限公司 | 一种免疫细胞培养基、培养方法及应用 |
CN105462924A (zh) * | 2015-12-04 | 2016-04-06 | 广州赛莱拉干细胞科技股份有限公司 | Nk细胞的培养方法及无血清培养基组合 |
CN113564119A (zh) * | 2021-09-03 | 2021-10-29 | 东莞再立健生物科技有限公司 | 一种nk细胞培养基及nk细胞培养方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102978160A (zh) * | 2012-12-13 | 2013-03-20 | 上海柯莱逊生物技术有限公司 | 一种体外诱导扩增nkt细胞的方法 |
CN102994449A (zh) * | 2012-12-13 | 2013-03-27 | 上海柯莱逊生物技术有限公司 | 一种体外扩增nk细胞的方法 |
-
2014
- 2014-03-27 CN CN201410118900.6A patent/CN103849600A/zh active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102978160A (zh) * | 2012-12-13 | 2013-03-20 | 上海柯莱逊生物技术有限公司 | 一种体外诱导扩增nkt细胞的方法 |
CN102994449A (zh) * | 2012-12-13 | 2013-03-27 | 上海柯莱逊生物技术有限公司 | 一种体外扩增nk细胞的方法 |
Non-Patent Citations (1)
Title |
---|
MAILLIARD,ET AL.: "IL-18-induced CD83+CCR7+NK helper cell", 《JEM》 * |
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104152412A (zh) * | 2014-08-13 | 2014-11-19 | 赛业(苏州)生物科技有限公司 | 一种适用于临床的高效扩增nk细胞的培养体系 |
CN104152413A (zh) * | 2014-08-13 | 2014-11-19 | 赛业(苏州)生物科技有限公司 | 一种适用于临床的高效扩增nk细胞的培养体系的应用 |
CN104593324A (zh) * | 2014-11-28 | 2015-05-06 | 广州赛莱拉干细胞科技股份有限公司 | 一种自然杀伤细胞的培养基及自然杀伤细胞的扩增培养方法 |
CN104593324B (zh) * | 2014-11-28 | 2018-04-13 | 广州赛莱拉干细胞科技股份有限公司 | 一种自然杀伤细胞的培养基及自然杀伤细胞的扩增培养方法 |
CN104450614A (zh) * | 2014-12-05 | 2015-03-25 | 上海安集协康生物技术有限公司 | 一种无动物蛋白源的免疫细胞无血清培养基及其使用方法 |
CN104450614B (zh) * | 2014-12-05 | 2017-07-07 | 上海安集协康生物技术股份有限公司 | 一种无动物蛋白源的免疫细胞无血清培养基及其使用方法 |
CN104818248A (zh) * | 2015-03-25 | 2015-08-05 | 苏州佰通生物科技有限公司 | 一种免疫细胞培养基、培养方法及应用 |
CN105462924A (zh) * | 2015-12-04 | 2016-04-06 | 广州赛莱拉干细胞科技股份有限公司 | Nk细胞的培养方法及无血清培养基组合 |
CN105462924B (zh) * | 2015-12-04 | 2019-01-18 | 广州赛莱拉干细胞科技股份有限公司 | Nk细胞的培养方法及无血清培养基组合 |
CN113564119A (zh) * | 2021-09-03 | 2021-10-29 | 东莞再立健生物科技有限公司 | 一种nk细胞培养基及nk细胞培养方法 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103849600A (zh) | 一种适用于培养nk细胞的无血清培养基 | |
CN107922925B (zh) | 用于自然杀伤细胞扩增的方法 | |
CN105462924B (zh) | Nk细胞的培养方法及无血清培养基组合 | |
Sadeghi et al. | Large-scale bioreactor expansion of tumor-infiltrating lymphocytes | |
CN101519646B (zh) | 一种cik细胞及其制备方法和细胞制剂 | |
CN104371974B (zh) | 一种自体外周血淋巴细胞cik的培养方法 | |
CN104357390A (zh) | 同时高效扩增cd3+cd56+cik细胞和cd3-cd56+nk细胞的方法 | |
CN103834615A (zh) | 一种适用于培养γδT细胞的无血清培养基 | |
CN108699529A (zh) | 用于培养含癌症干细胞(csc)的细胞群的化学成分确定的培养基 | |
CN104894065A (zh) | 一种nk细胞培养基及nk细胞的培养方法 | |
CN109422815A (zh) | 双特异性嵌合抗原受体c-Met/PD-1 scFv-CAR-T及其构建方法和应用 | |
CN103436583B (zh) | 一种生产治疗性犬瘟热病毒单克隆抗体的方法、其制品及杂交瘤细胞 | |
CN104073463A (zh) | 一种支持cho高密度悬浮培养的无血清无蛋白培养基 | |
CN104087558A (zh) | 一种杂交瘤细胞无血清培养基 | |
CN104152412A (zh) | 一种适用于临床的高效扩增nk细胞的培养体系 | |
Jones et al. | Emerging challenges in cell therapy manufacturing | |
CN103160458A (zh) | 一种适合Vero细胞生长的低血清培养基 | |
CN107904175B (zh) | 用于免疫细胞的诱导扩增的培养箱 | |
CN108085342A (zh) | 一种嵌合抗原受体基因修饰的t淋巴细胞的制备方法、制得的car-t细胞及其用途 | |
CN104152413A (zh) | 一种适用于临床的高效扩增nk细胞的培养体系的应用 | |
CN106085957A (zh) | 一种微载体三维扩增并激活自然杀伤细胞的方法 | |
CN105779375A (zh) | 一种新型无血清培养基 | |
CN104726400A (zh) | 人多能干细胞向生殖细胞分化的无动物源组分培养方法 | |
CN102268407B (zh) | 一种rhIL-12工程细胞大规模无血清培养方法 | |
CN102327609B (zh) | 一种乙型脑炎疫苗的生产方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
ASS | Succession or assignment of patent right |
Owner name: SHANGHAI CLAISON BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: YE YONGQING Effective date: 20140902 |
|
C10 | Entry into substantive examination | ||
C41 | Transfer of patent application or patent right or utility model | ||
C53 | Correction of patent of invention or patent application | ||
CB03 | Change of inventor or designer information |
Inventor after: Ye Yongqing Inventor after: Chen Xinxi Inventor before: Ye Yongqing |
|
COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: YE YONGQING TO: YE YONGQING CHEN XINXI Free format text: CORRECT: ADDRESS; FROM: 350000 FUZHOU, FUJIAN PROVINCE TO: 200120 PUDONG NEW AREA, SHANGHAI |
|
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20140902 Address after: 200120 Shanghai city Pudong New Area college town right Li No. 1628 building 4 room 3072 Applicant after: SHANGHAI CLAISON BIOTECHNOLOGY CO.,LTD. Address before: 350000, 338 Fu Cheng garden, Hualin Road, Fujian, Fuzhou, 13 71D Applicant before: Ye Yongqing |
|
C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140611 |