CN103843800A - Sterilization composition and application - Google Patents
Sterilization composition and application Download PDFInfo
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- CN103843800A CN103843800A CN201210514440.XA CN201210514440A CN103843800A CN 103843800 A CN103843800 A CN 103843800A CN 201210514440 A CN201210514440 A CN 201210514440A CN 103843800 A CN103843800 A CN 103843800A
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- cyazofamid
- bactericidal
- carbamates
- agent
- propineb
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Abstract
The invention belongs to the technical field of agricultural bactericides, and particularly relates to a sterilization composition containing cyazofamid and sulfo-carbamic acid salt bactericide compound and application of the sterilization composition. The sterilization composition contains cyazofamid and at least one sulfo-carbamic acid salt bactericide, wherein the weight ratio of the cyazofamid to the sulfo-carbamic acid salt bactericide is (100:1)-(1:100). The agricultural bactericide is used for preventing and curing disease caused by pathogenic fungus, particularly suitable for preventing and curing downy mildew and epidemic disease on multiple crops of melon and fruits and vegetables, achieves the effect of synergistic interaction, and has good effect for overcoming and delaying drug resistance of pathogenic bacteria.
Description
Technical field
The invention belongs to the technical field of disinfectant use in agriculture, be specifically related to a kind of contain cyazofamid and composite bactericidal composition and the application of Carbamates bactericidal agent.
Background technology
The chloro-2-cyano group-5-of cyazofamid (Cyazofamid) chemical name 4-p-methylphenyl-imidazoles-1-N, N-dimethyl methyl acid amides, belongs to imidazoles bactericide, is mitochondrial respiratory inhibitor, the all vegetative stages of oomycetes are all had to effect, the germ that metalaxyl is produced to resistance or sensitivity all has activity, has certain interior suction and therapeutic activity, and the lasting period is long, resistance of rainwater washing against, to crop, the mankind, Environmental security, but long-term single use can make germ develop immunity to drugs to it unavoidably.
Carbamates bactericidal agent comprises mancozeb (Mancozeb), the complex salt of chemical name ethylidene dithiocarbamate manganese and zinc; Zineb (Zineb), chemical name zinc ethylene bisdithiocarbamate; Carbatene (Metriam), chemical name three [ammonia (two (the two thiocarbamate esters) zinc (2+) of ethene)] [8-connects dithione for tetrahydrochysene-Isosorbide-5-Nitrae, 7-bis-thiophene diazocine-3], polymer; Propineb (Propineb), chemical name acrylic bisdithiocarbamic zinc; Tmtd (Thiram), chemical name tetramethyl thiuram disulfide; The bactericide of this class belongs to the wider protective fungicide of fungicidal spectrum; there is good prolection; but the therapeutic activity of this series bactericidal agent is very weak; result for the treatment of is not obvious, uses separately that not only quick-acting is poor, and easily produces resistance; produce little effect; peasant considers from the angle of economy and effect, uses seldom separately, thereby its use is subject to certain restrictions.The current composite report that has no about cyazofamid and Carbamates bactericidal agent.
Summary of the invention
Object of the present invention is exactly the problem for above-mentioned existence, for fear of the problem such as resistance generation, drug effect reduction that uses cyazofamid single dose to bring, provide a kind of and contained cyazofamid and Carbamates bactericidal agent carries out composite bactericidal composition, can be used to the multiple diseases that control is caused by disease fungus, be particularly useful for preventing and treating downy mildew, the epidemic disease etc. on fruit, vegetables, cereal crop, and can play the effect of Synergistic, the pesticide resistance overcoming and delay pathogen is also had to good effect.
In the present invention, the cyazofamid of mentioning, the weight of Carbamates bactericidal agent and the gross weight of the two etc., all refer to the weight after folding hundred, i.e. the weight of active principle.
Bactericidal composition of the present invention, contains cyazofamid and at least one Carbamates bactericidal agent, and described cyazofamid and the weight ratio of Carbamates bactericidal agent be, cyazofamid: Carbamates bactericidal agent=100:1～1:100.
Wherein, described cyazofamid and the weight ratio of Carbamates bactericidal agent be, cyazofamid: Carbamates bactericidal agent=1:5～1:20.
In bactericidal composition of the present invention, described Carbamates bactericidal agent is mancozeb, zineb, Propineb, Carbatene or tmtd.
In this bactericidal composition, the total weight percentage of described cyazofamid and Carbamates bactericidal agent is 0.5%～80%.
This bactericidal composition formulation comprises suspending agent, wetting powder and water dispersible granules, adds auxiliary agent and excipient, and the production technology of all knowing according to those skilled in the art can be made into needed formulation.
This bactericidal composition, for preventing and treating the disease being caused by disease fungus, is particularly useful for preventing and treating downy mildew, the epidemic disease etc. in the various crop such as melon and fruit, vegetables.
Beneficial effect of the present invention is: on the one hand, cyazofamid and Carbamates bactericidal agent are carried out composite, not only make composition have protection and therapeutic activity concurrently; improve quick-acting; extend the protection period, and shown good synergistic effect in certain ratio range, compared with single dose; obviously improve bactericidal effect; and as a kind of compound medicine, easy to use, thus reduce medicament usage amount; in reducing peasant's drug cost, reduce influence degree.On the other hand, two kinds of active substance structures in composition differ larger, do not have cross resistance, and mixture is used the resistance that can effectively delay single dose to occur and development, thereby have extended the life cycle of single dose.In a word, this combination sterilization composition, effectively combines both advantage, has played synergistic effect, obviously improves result for the treatment of, effectively delaying drug resistance problem.
Study the indoor virulence of cyazofamid from the mixture Phytophthora infestans of the different proportionings of Carbamates bactericidal agent with mycelial growth rate method, experimental technique adopts the abundant method of Sun Yun to calculate co-toxicity coefficient (CTC) and evaluates mixed effect, and in the time that CTC value is greater than 120, mixture has synergistic effect.Concrete grammar is as follows:
Get the liquid preparing and be placed in 100ml 50-60 DEG C of PDA sterilising medium, shake up, pour in the sterilizing culture dish of diameter 60 mm, after cooled and solidified, move respectively the bacterium cake that length is consistent, diameter is 4mm of delivering a child, to add the sterilising medium of equivalent coordinative solvent as blank, all operations all carries out sterile working at superclean bench, and 3 repetitions are established in every processing.Put into 27 DEG C of constant incubators and cultivate after 3-8 days, adopt right-angled intersection method to measure colony diameter, calculate the relevant parameters such as bacteriostasis rate, EC50, CTC, relevant calculating formulas is as follows:
Pure amount of growth=bacterium colony average diameter-bacterium cake diameter (0.4cm)
Bacteriostasis rate=[the pure amount of growth of (contrast pure amount of growth-process pure amount of growth) × 100/ contrast] × 100%
Toxicity index TI(B) EC50*100 of EC50/B agent of=standard agent A
The EC50*100 of the EC50/AB of actual toxicity index ATI (AB)=A
Theoretical toxicity index TTI (the AB)=percentage of percentage+TI (B) the * B of TI (A) * A in mixture in mixture
Co-toxicity coefficient CTC=ATI(AB)/TTI(AB) * 100
Experimental result is as follows:
The Toxicity Determination of table 1 cyazofamid and the mixture Phytophthora infestans of the different proportionings of Propineb
| Reagent agent | Two kinds of active component ratios | EC50(ppm) | CTC |
| Cyazofamid | —— | 1.35 | —— |
| Propineb | —— | 4.18 | —— |
| Cyazofamid: Propineb | 100：1 | 2.21 | 122.99 |
| Cyazofamid: Propineb | 80：1 | 1.62 | 168.07 |
| Cyazofamid: Propineb | 40：1 | 1.28 | 214.47 |
| Cyazofamid: Propineb | 20：1 | 1.43 | 195.10 |
| Cyazofamid: Propineb | 10：1 | 1.62 | 177.59 |
| Cyazofamid: Propineb | 5：1 | 1.61 | 189.03 |
| Cyazofamid: Propineb | 1：1 | 1.78 | 229.31 |
| Cyazofamid: Propineb | 1：5 | 2.02 | 306.70 |
| Cyazofamid: Propineb | 1：10 | 2.18 | 322.10 |
| Cyazofamid: Propineb | 1：20 | 2.89 | 263.01 |
| Cyazofamid: Propineb | 1：40 | 5.45 | 145.93 |
| Cyazofamid: Propineb | 1：80 | 4.26 | 191.29 |
| Cyazofamid: Propineb | 1:100 | 5.46 | 150.0 |
From table 1, cyazofamid and Propineb carry out composite, Phytophthora infestans has good indoor virulence, the CTC value of all proportionings all exceedes 120, show that these two bactericide have good synergistic function under this proportioning, and in the time that mixed ratio is between 1: 5～1: 20, synergy is particularly remarkable.
When cyazofamid and mancozeb, zineb, Carbatene or tmtd carry out combine experiment with aforementioned proportion, show the synergy rule identical with Propineb, under the ratio of 1:5～1:20, the synergistic effect of Phytophthora infestans is more obvious.
Embodiment
Below in conjunction with embodiment, bactericidal composition of the present invention is described further, but the present invention is not limited thereto.
Embodiment 1: cyazofamid and the Antibacterial Activity of zineb list mixture to Chinese cabbage Pseudoperonospora cubensis
Test method: adopt greenhouse pot culture Chinese cabbage seedling to the 2-3 leaf phase, carry out foliar spray processing by designer drug agent dose, contrast spray equivalent clear water.After 24 hours, inoculate Chinese cabbage downy mildew bacterium, under moisturizing condition, cultivate, investigation result in the time that the blank of not dispenser is fully fallen ill, test result is in table 2.
Table 2 cyazofamid and the Antibacterial Activity of zineb list mixture to Chinese cabbage Pseudoperonospora cubensis
As shown in Table 2, cyazofamid and zineb are composite, and Chinese cabbage Pseudoperonospora cubensis is had to good indoor bacteriostatic activity, and higher than the bacteriostatic activity of single dose.
Embodiment 2: cyazofamid and the Antibacterial Activity of mancozeb list mixture to Plasmopara viticola
Adopt greenhouse cuttage grape seedling, measure cyazofamid, the fungistatic effect of mancozeb list mixture to Plasmopara viticola.In the time that grape seedling grows to 3～4 leaves, select 2 leaves of growth uniformity, adopt manual sprayer to carry out blade face reagent spray processing, contrast spray equivalent clear water.After 24 hours, inoculate pathogen, under moisturizing condition, cultivate 7d " Invest, Then Investigate " result, the results are shown in Table 3.
Table 3 cyazofamid and the Antibacterial Activity of mancozeb list mixture to Plasmopara viticola
As shown in Table 3, cyazofamid and mancozeb are composite, Plasmopara viticola is had to good indoor bacteriostatic activity, and mixed ratio bacteriostatic activity between 1:5～1:20 are higher.
Embodiment 3: the test of pesticide effectiveness of cyazofamid and Propineb list mixture control tomato late blight
74% cyazofamid Wettable propineb powder, comprises following component by weight:
Cyazofamid 4%
Propineb 70%
Neopelex 2.5%
White Carbon black 10%
Wooden calcium 4%
Pull open powder 3%
K12 3%
Precipitated calcium carbonate surplus
Said components is fully mixed by formula rate, after ultra-fine pulverizer disintegrating, obtain 74% cyazofamid Wettable propineb powder.
Adopt greenhouse pot culture tomato seedling, measure cyazofamid, Propineb single dose and the control efficiency of above-mentioned mixture to tomato late blight.In the time that tomato grows to 3～4 leaves, select the blade of growing way uniformity to adopt manual sprayer to carry out foliar spray processing, contrast spray equivalent clear water.After 24 hours, inoculate pathogen, 7d " Invest, Then Investigate " result is cultivated in moisturizing, and result of the test is in table 4.
The test of pesticide effectiveness result of table 4 cyazofamid and Propineb list mixture control tomato late blight
As can be seen from Table 4, the mixture preparation of cyazofamid and Propineb can effectively be prevented and treated tomato late blight, and is better than the control efficiency of single dose.
Embodiment 4: the test of pesticide effectiveness of cyazofamid and Carbatene list mixture control cucumber downy mildew
12% cyazofamid Carbatene water dispersible granules, comprises following component by weight:
Cyazofamid 2%
Carbatene 10%
Methanonaphthalene sodium sulfonate 3%
Neopelex 7%
Polyvinylpyrrolidone 2.5%
Attapulgite surplus
By said components co-grinding, after adding water and mediating, pack granulation in comminutor into, then drying, screening make 12% cyazofamid Carbatene water dispersible granules.
Adopt greenhouse pot culture cucumber seedling, measure cyazofamid, Carbatene single dose and the control efficiency of above-mentioned mixture to cucumber downy mildew.In the time that cucumber grows to 3～4 leaves, select the blade of growing way uniformity to adopt manual sprayer to carry out foliar spray processing, contrast spray equivalent clear water.After 24 hours, inoculate pathogen, 7d " Invest, Then Investigate " result is cultivated in moisturizing, and result of the test is in table 5.
The test of pesticide effectiveness result of table 5 cyazofamid and Carbatene list mixture control cucumber downy mildew
As can be seen from Table 5, the mixture preparation of cyazofamid and Carbatene can effectively be prevented and treated cucumber downy mildew, and is better than the control efficiency of single dose.
Embodiment 5: the test of pesticide effectiveness of cyazofamid and tmtd list mixture control peronophythora litchi
33% cyazofamid tmtd suspending agent, comprises following component by weight:
Cyazofamid 3%
Tmtd 30%
Methyl naphthalene sulfonate formaldehyde condensate 5.5%
White Carbon black 5%
Aluminium-magnesium silicate 2%
Ethylene glycol 10%
Tributyl phosphate 0.1%
Diatomite 5%
Water surplus
Above-mentioned former medicine, various auxiliary agent and filler etc. are added in sand milling still by formula rate, carry out porphyrize, make 33% cyazofamid tmtd suspending agent.
In field test cyazofamid, tmtd single dose and the control efficiency of above-mentioned mixture to peronophythora litchi.Dispenser in the time of lichee field first meeting scab, dispenser 3 times altogether, interval 7d, 8d investigation result after the 3rd dispenser, result of the test is in table 6.
The test of pesticide effectiveness result of table 6 cyazofamid and tmtd list mixture control peronophythora litchi
As can be seen from Table 6, the mixture preparation of cyazofamid and tmtd can effectively be prevented and treated peronophythora litchi, and is better than the control efficiency of single dose.
Claims (7)
1. a bactericidal composition, contains cyazofamid and at least one Carbamates bactericidal agent, and described cyazofamid and the weight ratio of Carbamates bactericidal agent be, cyazofamid: Carbamates bactericidal agent=100:1～1:100.
2. bactericidal composition according to claim 1, is characterized in that, described cyazofamid and the weight ratio of Carbamates bactericidal agent be, cyazofamid: Carbamates bactericidal agent=1:5～1:20.
3. bactericidal composition according to claim 1 and 2, is characterized in that, described Carbamates bactericidal agent is mancozeb, zineb, Propineb, Carbatene or tmtd.
4. bactericidal composition according to claim 3, is characterized in that, the total weight percentage of described cyazofamid and Carbamates bactericidal agent is 0.5%～80%.
5. bactericidal composition according to claim 4, is characterized in that, this bactericidal composition formulation comprises suspending agent, wetting powder and water dispersible granules.
6. bactericidal composition as claimed in claim 1, for preventing and treating the disease being caused by disease fungus.
7. bactericidal composition as claimed in claim 6, for preventing and treating downy mildew, the epidemic disease in the various crop such as melon and fruit, vegetables.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210514440.XA CN103843800A (en) | 2012-12-05 | 2012-12-05 | Sterilization composition and application |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210514440.XA CN103843800A (en) | 2012-12-05 | 2012-12-05 | Sterilization composition and application |
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| CN103843800A true CN103843800A (en) | 2014-06-11 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015079334A1 (en) * | 2013-11-26 | 2015-06-04 | Upl Limited | A method for controlling rust |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015079334A1 (en) * | 2013-11-26 | 2015-06-04 | Upl Limited | A method for controlling rust |
| GB2532700A (en) * | 2013-11-26 | 2016-05-25 | Upl Ltd | A method for controlling rust |
| GB2532700B (en) * | 2013-11-26 | 2016-08-31 | Upl Ltd | A method for controlling rust |
| US9788544B2 (en) | 2013-11-26 | 2017-10-17 | Upl Limited | Method for controlling rust |
| US10362782B2 (en) | 2013-11-26 | 2019-07-30 | Upl Limited | Method for controlling rust |
| US10694745B2 (en) | 2013-11-26 | 2020-06-30 | Upl Limited | Method for controlling rust |
| US10694744B2 (en) | 2013-11-26 | 2020-06-30 | Upl Limited | Method for controlling rust |
| US10701935B2 (en) | 2013-11-26 | 2020-07-07 | Upl Limited | Method for controlling rust |
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Application publication date: 20140611 |