CN103843582A - Technique for selecting and culturing bacterial strains for flammulina velutipes - Google Patents
Technique for selecting and culturing bacterial strains for flammulina velutipes Download PDFInfo
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- CN103843582A CN103843582A CN201310753558.2A CN201310753558A CN103843582A CN 103843582 A CN103843582 A CN 103843582A CN 201310753558 A CN201310753558 A CN 201310753558A CN 103843582 A CN103843582 A CN 103843582A
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Abstract
The invention relates to the field of agriculture. A technique for selecting and culturing bacterial strains for flammulina velutipes comprises culture medium preparation, strain breeding, inoculation and regulation and control for the growing environment. The regulation and control for the growing environment comprises the steps of: controlling an indoor growing temperature to be at 4.5-5.5 DEG C, controlling the humidity to be at 97%-99%, controlling the volume ratio concentration of CO2 to be at 8000PPM-12000PPM, and controlling the total illumination time of the flammulina velutipes in the whole life cycle to be 38-42 hours. Under the growing environment, the grown flammulina velutipes are more uniform, the difference among individuals is smaller, the mushroom cap is difficult to amplify, the mushroom stem is tall and straight, the mushroom roots are compact and the appearance of the products is excellent.
Description
Technical field
The present invention relates to agriculture field, relate in particular to the culture technology of Asparagus.
Background technology
When plantation bacterium mushroom, indoor a lot of parameters all need flexible adjustment, are beneficial to the growth of bacterium mushroom, particularly Asparagus.How to choose indoor environment parameter, could acquisition product mutually preferably Asparagus Shi Ge great Asparagus manufacturer constantly exploring always.
Summary of the invention
The object of the present invention is to provide a kind of Asparagus to select strain culturing technique, the Asparagus product that adopt this culture technology to obtain are mutually better.
Technical problem solved by the invention can realize by the following technical solutions:
Asparagus is selected strain culturing technique, comprises medium preparation, strain improvement, inoculation, growing environment regulation and control, it is characterized in that:
Described growing environment regulation and control comprise:
Control growth indoor temperature and be 4.5 degrees Celsius~5.5 degrees Celsius, humidity and be 97%~99%, CO
2volume by volume concentration be 8000PPM~12000PPM;
Total light application time of controlling the whole growth cycle of Asparagus is 38 hours~42 hours.
Under above-mentioned growing environment, the Asparagus of growth is more even, and each interindividual variation is less, and mushroom cap is not easy to amplify, uprightly tall and straight, mushroom root compactness of mushroom handle, and product are very good mutually.
In the time of inoculation, in culture bottle, the moisture of medium is controlled at 66%-68%.Experiment shows, in moisture at 66%-68% in the situation that, is particularly conducive to the uniformity of mushroom handle, mushroom lid 67% time, and can promotes once to sprout, and can ensure more consolidation of root.
When strain improvement, in the Asparagus growing under above-mentioned growing environment, selection good bacterial strain mutually, separates cultivation by tissue, makes its stability of characteristics, as bacterial classification.
As a kind of preferred version, the temperature of described growth room is that 5 DEG C, humidity are 98%, carbonic acid gas volume by volume concentration is that total light application time in 10000ppm, the whole growth cycle of Asparagus is 40 hours;
In the time of inoculation, in culture bottle, the moisture of medium is 67%.Under this scheme, Asparagus product are mutually better, and evenly, and output is higher.
When described growing environment regulation and control, select strain culturing environment balance control system to regulate and control by Asparagus, Asparagus is selected strain culturing environment balance control system, comprise a growth room of cultivating for Asparagus, in described growth room, be provided with the information acquisition module of a collection growth room environmental parameter, the signal output part of described information acquisition module connects a master control system, and described master control system connects a calculator;
Described information acquisition module comprises temperature sensor for measuring growth room's temperature information, for measuring the humidity sensor of humidity information in growth room, for measuring the light sensor of light conditions in growth room, for measuring the carbon dioxide sensor of gas concentration lwevel in growth room, described temperature sensor, described humidity sensor, described light sensor, described carbon dioxide sensor all connect described master control system.
The present invention can gather the information of temperature in growth room, humidity, illumination, gas concentration lwevel by information acquisition module, and send to master control system, make user pass through calculator and can understand in real time the environmental parameter in growth room, in time environmental parameter is made adjustment.
Asparagus is selected strain culturing environment balance control system, also comprise an environmental parameter regulating system, described environmental parameter regulating system comprises a humidifier of adjusting for growth room's humidity, in growth room, be provided with one for placing the bedstead of blake bottle, described bedstead from bottom to top, at least be provided with six layers, the gas outlet of each humidifier is all communicated with Jun Gu growth room by least one humidification pipeline; The end of giving vent to anger of described humidification pipeline is arranged between the top of described bedstead and the second high level;
Described humidifier connects described master control system;
When humidity is higher than 99% time, deep bead governor motion is opened deep bead described in calculator or master control system control, new air is entered, thereby reduce indoor humidity, and when humidity is lower than 97% time, humidifier is opened in calculator or master control system control, and humidity is increased.Can realize the automatic adjustment of humidity by these means.
Described environmental parameter regulating system also comprises a new wind passage ventilating for growth room, the air inlet of described new wind passage and extraneous UNICOM, the gas outlet of described new wind passage and UNICOM of growth room, the gas outlet place of described new wind passage is provided with a part sieve oxygenerator, and described molecular-sieve oxygen generator connects described master control system;
When humidity is during higher than 12000ppm, calculator or master control system are opened molecular-sieve oxygen generator, thereby increase indoor oxygen content, thereby reduce indoor carbon dioxide content, humidity is during lower than 8000ppm, calculator or master control system closure molecule sieve oxygenerator, Growth of Flammulina Velutipes is breathed gas concentration lwevel is increased.The oneself that can realize gas concentration lwevel by these means adjusts.
Described environmental parameter regulating system also comprises an air-cooler for growth room's refrigeration, and the gas outlet of described air-cooler is positioned at described growth room, and described air-cooler connects described master control system;
When temperature is during higher than 5.5 DEG C, calculator or master control system control air-cooler are started working, and temperature is declined, and when temperature is during lower than 4.5 DEG C, calculator or master control system are closed air-cooler, and the growth of bacterium mushroom produces heat, makes temperature rise.Can realize the adjustment voluntarily of temperature by these means.
Described environmental parameter regulating system also comprises growth room's sun shield, and described growth room sun shield is positioned at the light inlet window place of growth room, and described growth room sun shield connects a sun shield governor motion, and described sun shield governor motion connects described master control system;
In the time that light application time exceedes 45 hours, sun shield governor motion cuts out growth room's sun shield described in calculator or master control system control, makes Asparagus avoid illumination.The oneself that can realize light conditions by these means adjusts.
As a kind of preferred version, the mode of controlled light is to adopt the mode of repeatedly irradiating in short-term, and repeatedly irradiation time is controlled at irradiation per hour once, in once irradiating 3.5 minutes;
Or irradiation time is controlled at and is irradiated once per half an hour, and each irradiation time was controlled in 1.8 minutes;
Or irradiation time is controlled to every 2 hours and irradiates once, each irradiation time was controlled in 7 minutes.
Embodiment
For technological means, creation characteristic that the present invention is realized, reach object and effect is easy to understand, below further set forth the present invention.
Asparagus is selected strain culturing technique, comprises medium preparation, strain improvement, inoculation, growing environment regulation and control, and growing environment regulation and control comprise: controlling growth indoor temperature and be 4.5 degrees Celsius~5.5 degrees Celsius, humidity is 97%~99%CO
2volume by volume concentration be 8000PPM~12000PPM; Total light application time of controlling the whole growth cycle of Asparagus is 38 hours~42 hours.Under above-mentioned growing environment, the Asparagus of growth is more even, and each interindividual variation is less, and mushroom cap is not easy to amplify, uprightly tall and straight, mushroom root compactness of mushroom handle, and product are very good mutually.
In when inoculation, in culture bottle, the moisture of medium is controlled at 66%-68%, and preferably 67%.Experiment shows, in moisture at 66%-68% in the situation that, is particularly conducive to the uniformity of mushroom handle, mushroom lid 67% time, and can promotes once to sprout, and can ensure more consolidation of root.
When strain improvement, in the Asparagus growing under above-mentioned growing environment, selection good bacterial strain mutually, separates cultivation by tissue, makes its stability of characteristics, as bacterial classification.
When growing environment regulation and control, can select strain culturing environment balance control system to regulate and control by Asparagus, Asparagus is selected strain culturing environment balance control system, comprise a growth room of cultivating for Asparagus, in growth room, be provided with the information acquisition module of a collection growth room environmental parameter, the signal output part of information acquisition module connects a master control system, and master control system connects a calculator; Information acquisition module comprises temperature sensor for measuring growth room's temperature information, for measuring the humidity sensor of humidity information in growth room, for measuring the light sensor of light conditions in growth room, for measuring the carbon dioxide sensor of gas concentration lwevel in growth room, temperature sensor, humidity sensor, light sensor, carbon dioxide sensor all connect master control system.The present invention can gather the information of temperature in growth room, humidity, illumination, gas concentration lwevel by information acquisition module, and send to master control system, make user pass through calculator and can understand in real time the environmental parameter in growth room, in time environmental parameter is made adjustment.
Asparagus is selected strain culturing environment balance control system, also comprise an environmental parameter regulating system, environmental parameter regulating system comprises a humidifier of adjusting for growth room's humidity, in growth room, be provided with one for placing the bedstead of blake bottle, bedstead from bottom to top, at least be provided with six layers, the gas outlet of each humidifier is all communicated with Jun Gu growth room by least one humidification pipeline; The end of giving vent to anger of humidification pipeline is arranged between the top of bedstead and the second high level; Humidifier connects master control system.User can pass through the working condition of calculator or master control system control humidifier, and then adjusts the humidity in growth room.
Environmental parameter regulating system also comprises a new wind passage ventilating for growth room, air inlet and the extraneous UNICOM of new wind passage, gas outlet and the UNICOM of growth room of new wind passage, the gas outlet place of new wind passage is provided with a part sieve oxygenerator, and molecular-sieve oxygen generator connects master control system.User can pass through calculator or master control system control molecular-sieve oxygen generator, and then adjusts gas concentration lwevel by the oxygen concentration of adjusting in growth room.
Environmental parameter regulating system also comprises an air-cooler for growth room's refrigeration, and the gas outlet of air-cooler is positioned at growth room, and air-cooler connects master control system.User can pass through the working condition of calculator or master control system control air-cooler, and then adjusts the temperature in growth room.
Environmental parameter regulating system also comprises growth room's sun shield, and growth room's sun shield is positioned at the light inlet window place of growth room, and growth room's sun shield connects a sun shield governor motion, and sun shield governor motion connects master control system.User can pass through calculator or master control system control sun shield governor motion, and then adjusts the state of growth room's sun shield, thereby controls the logical light situation of growth room.
Preferably 5 DEG C of the temperature of growth room.When temperature is during higher than 5.5 DEG C, calculator or master control system control air-cooler are started working, and temperature is declined, and when temperature is during lower than 4.5 DEG C, calculator or master control system are closed air-cooler, and the growth of bacterium mushroom produces heat, makes temperature rise.
The humidity of growth room preferably 98%.When humidity is higher than 99% time, calculator or master control system control deep bead governor motion are opened deep bead, and new air is entered, thereby reduce indoor humidity.When humidity is lower than 97% time, humidifier is opened in calculator or master control system control, and humidity is increased.
The gas concentration lwevel of growth room is preferred 10000ppm.When humidity is during higher than 12000ppm, calculator or master control system are opened molecular-sieve oxygen generator, thereby increase indoor oxygen content, thereby reduce indoor carbon dioxide content.When humidity is during lower than 8000ppm, calculator or master control system closure molecule sieve oxygenerator, Growth of Flammulina Velutipes is breathed gas concentration lwevel is increased.
Total light application time in the whole growth cycle of Asparagus preferably 40 hours, in the time that light application time exceedes 45 hours, calculator or master control system control sun shield governor motion are closed growth room's sun shield, make Asparagus avoid illumination.The mode of controlled light is to adopt the mode of repeatedly irradiating in short-term, and repeatedly irradiation time is controlled at irradiation per hour once, in once irradiating 3.5 minutes; Also irradiation time can be controlled at and irradiate once per half an hour, each irradiation time was controlled in 1.8 minutes; Also irradiation time can be controlled to every 2 hours and irradiate once, each irradiation time was controlled in 7 minutes.
Calculator is established a network interface, and calculator connects computer network by network interface.Equipment so at a distance just can be understood the ambient conditions in growth room by network, to make adjustment in time.
The signal output part of information acquisition module can connect master control system by a wireless communication module, preferably, and the signal output part wired connection wireless communication module of information acquisition module, wireless communication module wireless connections master control system.The present invention gathers the information of growth room's environmental parameter by information acquisition module like this, send to wirelessly master control system by wireless communication module, information acquisition module, wireless communication module, master control system, calculator and computer network form an Internet of things system.
Temperature sensor connects a temperature wireless transmitter module, and temperature sensor connects master control system by temperature wireless transmitter module.Humidity sensor connects a humidity wireless transmitter module, and humidity sensor connects master control system by humidity wireless transmitter module.Light sensor connects a light conditions wireless transmitter module, and light sensor connects master control system by light conditions wireless transmitter module.Carbon dioxide sensor connects a gas concentration lwevel wireless transmitter module, and carbon dioxide sensor connects master control system by gas concentration lwevel wireless transmitter module.
More than show and described general principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; that in above-described embodiment and specification, describes just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (10)
1. Asparagus is selected strain culturing technique, comprises medium preparation, strain improvement, inoculation, growing environment regulation and control, it is characterized in that:
Described growing environment regulation and control comprise:
Control growth indoor temperature and be 4.5 degrees Celsius~5.5 degrees Celsius, humidity and be 97%~99%, CO
2volume by volume concentration be 8000PPM~12000PPM;
Total light application time of controlling the whole growth cycle of Asparagus is 38 hours~42 hours.
2. Asparagus according to claim 1 is selected strain culturing technique, it is characterized in that: in the time of inoculation, in culture bottle, the moisture of medium is controlled at 66%-68%.
3. Asparagus according to claim 1 is selected strain culturing technique, it is characterized in that: when strain improvement, in the Asparagus growing under growing environment claimed in claim 1, selection good bacterial strain mutually, separates cultivation by tissue, make its stability of characteristics, as bacterial classification.
4. Asparagus according to claim 2 is selected strain culturing technique, it is characterized in that: the temperature of described growth room is that 5 DEG C, humidity are 98%, carbonic acid gas volume by volume concentration is that total light application time in 10000ppm, the whole growth cycle of Asparagus is 40 hours;
In the time of inoculation, in culture bottle, the moisture of medium is 67%.
5. select strain culturing technique according to the Asparagus described in claim 1,2,3 or 4, it is characterized in that: when described growing environment regulation and control, select strain culturing environment balance control system to regulate and control by Asparagus, Asparagus is selected strain culturing environment balance control system, comprise a growth room of cultivating for Asparagus, in described growth room, be provided with the information acquisition module of a collection growth room environmental parameter, the signal output part of described information acquisition module connects a master control system, and described master control system connects a calculator;
Described information acquisition module comprises temperature sensor for measuring growth room's temperature information, for measuring the humidity sensor of humidity information in growth room, for measuring the light sensor of light conditions in growth room, for measuring the carbon dioxide sensor of gas concentration lwevel in growth room, described temperature sensor, described humidity sensor, described light sensor, described carbon dioxide sensor all connect described master control system.
6. Asparagus according to claim 5 is selected strain culturing technique, it is characterized in that: Asparagus is selected strain culturing environment balance control system, also comprise an environmental parameter regulating system, described environmental parameter regulating system comprises a humidifier of adjusting for growth room's humidity, in growth room, be provided with one for placing the bedstead of blake bottle, described bedstead, is at least provided with six layers from bottom to top, and the gas outlet of each humidifier is all communicated with Jun Gu growth room by least one humidification pipeline; The end of giving vent to anger of described humidification pipeline is arranged between the top of described bedstead and the second high level;
Described humidifier connects described master control system;
When humidity is higher than 99% time, deep bead governor motion is opened deep bead described in calculator or master control system control, new air is entered, thereby reduce indoor humidity, and when humidity is lower than 97% time, humidifier is opened in calculator or master control system control, and humidity is increased.
7. Asparagus according to claim 6 is selected strain culturing technique, it is characterized in that: described environmental parameter regulating system also comprises a new wind passage ventilating for growth room, the air inlet of described new wind passage and extraneous UNICOM, the gas outlet of described new wind passage and UNICOM of growth room, the gas outlet place of described new wind passage is provided with a part sieve oxygenerator, and described molecular-sieve oxygen generator connects described master control system;
When humidity is during higher than 12000ppm, calculator or master control system are opened molecular-sieve oxygen generator, thereby increase indoor oxygen content, thereby reduce indoor carbon dioxide content, humidity is during lower than 8000ppm, calculator or master control system closure molecule sieve oxygenerator, Growth of Flammulina Velutipes is breathed gas concentration lwevel is increased.
8. Asparagus according to claim 7 is selected strain culturing technique, it is characterized in that: described environmental parameter regulating system also comprises an air-cooler for growth room's refrigeration, the gas outlet of described air-cooler is positioned at described growth room, and described air-cooler connects described master control system;
When temperature is during higher than 5.5 DEG C, calculator or master control system control air-cooler are started working, and temperature is declined, and when temperature is during lower than 4.5 DEG C, calculator or master control system are closed air-cooler, and the growth of bacterium mushroom produces heat, makes temperature rise.
9. Asparagus according to claim 8 is selected strain culturing technique, it is characterized in that: described environmental parameter regulating system also comprises growth room's sun shield, described growth room sun shield is positioned at the light inlet window place of growth room, described growth room sun shield connects a sun shield governor motion, and described sun shield governor motion connects described master control system;
In the time that light application time exceedes 45 hours, sun shield governor motion cuts out growth room's sun shield described in calculator or master control system control, makes Asparagus avoid illumination.
10. Asparagus according to claim 9 is selected strain culturing technique, it is characterized in that: the mode of controlled light is to adopt the mode of repeatedly irradiating in short-term, and repeatedly irradiation time is controlled at irradiation per hour once, in once irradiating 3.5 minutes;
Or irradiation time is controlled at and is irradiated once per half an hour, and each irradiation time was controlled in 1.8 minutes;
Or irradiation time is controlled to every 2 hours and irradiates once, each irradiation time was controlled in 7 minutes.
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CN105165399A (en) * | 2015-09-15 | 2015-12-23 | 上海雪榕生物科技股份有限公司 | Method for adjusting production states of edible fungi through measurement of respiratory capacity of edible fungi |
CN105165400A (en) * | 2015-09-15 | 2015-12-23 | 上海雪榕生物科技股份有限公司 | Method for quick fruiting of after-ripening pleurotus nebrodensis at low temperature |
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CN105165400A (en) * | 2015-09-15 | 2015-12-23 | 上海雪榕生物科技股份有限公司 | Method for quick fruiting of after-ripening pleurotus nebrodensis at low temperature |
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CN105165400B (en) * | 2015-09-15 | 2018-07-27 | 上海雪榕生物科技股份有限公司 | The method of the quick fruiting of Pleurotus nebrodensis low temperature after-ripening |
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