CN103843582B - Strain culturing technique selected by Asparagus - Google Patents
Strain culturing technique selected by Asparagus Download PDFInfo
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- CN103843582B CN103843582B CN201310753558.2A CN201310753558A CN103843582B CN 103843582 B CN103843582 B CN 103843582B CN 201310753558 A CN201310753558 A CN 201310753558A CN 103843582 B CN103843582 B CN 103843582B
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Abstract
The present invention relates to agriculture field.Strain culturing technique selected by Asparagus, comprise medium preparing, strain improvement, inoculation, growing environment regulation and control, described growing environment regulation and control comprise: controlling the volume by volume concentration that growth indoor temperature is 4.5 degrees Celsius ~ 5.5 degrees Celsius, humidity is 97% ~ 99% carbonic acid gas is 8000PPM ~ 12000PPM; The total light application time controlling the whole growth cycle of Asparagus is 38 hours ~ 42 hours.Under above-mentioned growing environment, the Asparagus of growth is relatively more even, and each interindividual variation is less, and mushroom cap is not easy to amplify, mushroom handle uprightly tall and straight, mushroom root is compact, product are very good mutually.
Description
Technical field
The present invention relates to agriculture field, particularly relate to the culture technology of Asparagus.
Background technology
During plantation bacterium mushroom, indoor a lot of parameters all need flexible adjustment, are beneficial to the growth of bacterium mushroom, particularly Asparagus.How to select indoor environment parameter, could obtain product mutually preferably Asparagus Shi Ge great Asparagus manufacturer constantly to explore always.
Summary of the invention
The object of the present invention is to provide a kind of Asparagus to select strain culturing technique, the Asparagus product adopting this culture technology to obtain are mutually better.
Technical problem solved by the invention can realize by the following technical solutions:
Strain culturing technique selected by Asparagus, comprises medium preparing, strain improvement, inoculation, growing environment regulation and control, it is characterized in that:
Described growing environment regulation and control comprise:
Control growth indoor temperature is 4.5 degrees Celsius ~ 5.5 degrees Celsius, humidity is 97% ~ 99%, the volume by volume concentration of carbonic acid gas is 8000PPM ~ 12000PPM;
The total light application time controlling the whole growth cycle of Asparagus is 38 hours ~ 42 hours.
Under above-mentioned growing environment, the Asparagus of growth is relatively more even, and each interindividual variation is less, and mushroom cap is not easy to amplify, mushroom handle uprightly tall and straight, mushroom root is compact, product are very good mutually.
When inoculating, in culture bottle, the moisture of medium controls at 66%-68%.Experiment shows, when moisture at 66%-68%, particularly 67% time be conducive to the uniformity of mushroom handle, mushroom lid, and can promote once to sprout, and root more consolidation can be ensured.
During strain improvement, in the Asparagus grown under above-mentioned growing environment, selection good bacterial strain mutually, is separated by tissue and cultivates, make its stability of characteristics, as bacterial classification.
As a kind of preferred version, the temperature of described growth room is 5 DEG C, humidity is 98%, carbonic acid gas volume by volume concentration is 10000ppm, total light application time in the whole growth cycle of Asparagus is 40 hours;
When inoculating, in culture bottle, the moisture of medium is 67%.Under the program, Asparagus product are better mutually, and evenly, and output is higher.
During described growing environment regulation and control, strain culturing environment balance control system is selected to regulate and control by Asparagus, strain culturing environment balance control system selected by Asparagus, comprise a growth room of cultivating for Asparagus, the information acquisition module of a collection growth room environmental parameter is provided with in described growth room, the signal output part of described information acquisition module connects a master control system, and described master control system connects a calculator;
Described information acquisition module comprise temperature sensor for measuring growth room's temperature information, for measure growth room's humidity information humidity sensor, for measure light conditions in growth room light sensor, for measuring the carbon dioxide sensor of gas concentration lwevel in growth room, described temperature sensor, described humidity sensor, described light sensor, described carbon dioxide sensor all connect described master control system.
The present invention can gather the information of temperature in growth room, humidity, illumination, gas concentration lwevel by information acquisition module, and send to master control system, make user can be understood environmental parameter in growth room in real time by calculator, to make adjustment to environmental parameter in time.
Strain culturing environment balance control system selected by Asparagus, also comprise an environmental parameter regulating system, described environmental parameter regulating system comprises a humidifier adjusted for growth room's humidity, one is provided with for placing the bedstead of blake bottle in growth room, described bedstead from bottom to top, at least be provided with six layers, the gas outlet of each humidifier is all communicated with Jun Gu growth room by least one humidification pipeline; The end of giving vent to anger of described humidification pipeline is arranged between the top of described bedstead and the second high level;
Described humidifier connects described master control system;
When humidity higher than 99% time, calculator or master control system control described deep bead governor motion and open deep bead, and new air is entered, thus reduction indoor humidity, when humidity lower than 97% time, calculator or master control system control to open humidifier, and humidity is increased.The automatic adjustment of humidity can be realized by these means.
Described environmental parameter regulating system also comprises a new wind passage ventilated for growth room, the air inlet of described new wind passage and extraneous UNICOM, the gas outlet of described new wind passage and UNICOM of growth room, the gas outlet place of described new wind passage is provided with a part sieve oxygenerator, and described molecular-sieve oxygen generator connects described master control system;
When humidity is higher than 12000ppm, calculator or master control system open molecular-sieve oxygen generator, thus increase indoor oxygen content, thus reduce indoor carbon dioxide content, when humidity is lower than 8000ppm, calculator or master control system closure molecule sieve oxygenerator, Growth of Flammulina Velutipes is breathed and gas concentration lwevel is increased.Oneself's adjustment of gas concentration lwevel can be realized by these means.
Described environmental parameter regulating system also comprises an air-cooler freezed for growth room, and the gas outlet of described air-cooler is positioned at described growth room, and described air-cooler connects described master control system;
When temperature is higher than 5.5 DEG C, calculator or master control system control air-cooler and start working, and temperature is declined, and when temperature is lower than 4.5 DEG C, calculator or master control system close air-cooler, and the growth of bacterium mushroom produces heat, makes temperature increase.By these means can realize temperature from Row sum-equal matrix.
Described environmental parameter regulating system also comprises growth room's sun shield, and described growth room sun shield is positioned at the light inlet window place of growth room, and described growth room sun shield connects a sun shield governor motion, and described sun shield governor motion connects described master control system;
When light application time is constantly little more than 45, calculator or master control system control described sun shield governor motion and close growth room's sun shield, make Asparagus avoid illumination.Oneself's adjustment of light conditions can be realized by these means.
As a kind of preferred version, the mode of controlled light is adopt the mode of repeatedly irradiating in short-term, and repeatedly irradiation time controls in irradiation per hour once, and once irradiating is within 3.5 minutes;
Or irradiation time controlled to irradiate once in per half an hour, each irradiation time controls within 1.8 minutes;
Or irradiation time controlled to irradiate once at every 2 hours, each irradiation time controlled within 7 minutes.
Embodiment
The technological means realized to make the present invention, creation characteristic, reaching object and effect is easy to understand, setting forth the present invention further below.
Strain culturing technique selected by Asparagus, comprise medium preparing, strain improvement, inoculation, growing environment regulation and control, growing environment regulation and control comprise: controlling the volume by volume concentration that growth indoor temperature is 4.5 degrees Celsius ~ 5.5 degrees Celsius, humidity is 97% ~ 99% carbonic acid gas is 8000PPM ~ 12000PPM; The total light application time controlling the whole growth cycle of Asparagus is 38 hours ~ 42 hours.Under above-mentioned growing environment, the Asparagus of growth is relatively more even, and each interindividual variation is less, and mushroom cap is not easy to amplify, mushroom handle uprightly tall and straight, mushroom root is compact, product are very good mutually.
When inoculating, in culture bottle, the moisture of medium controls at 66%-68%, and preferably 67%.Experiment shows, when moisture at 66%-68%, particularly 67% time be conducive to the uniformity of mushroom handle, mushroom lid, and can promote once to sprout, and root more consolidation can be ensured.
During strain improvement, in the Asparagus grown under above-mentioned growing environment, selection good bacterial strain mutually, is separated by tissue and cultivates, make its stability of characteristics, as bacterial classification.
During growing environment regulation and control, strain culturing environment balance control system is selected to regulate and control by Asparagus, strain culturing environment balance control system selected by Asparagus, comprise a growth room of cultivating for Asparagus, the information acquisition module of a collection growth room environmental parameter is provided with in growth room, the signal output part of information acquisition module connects a master control system, and master control system connects a calculator; Information acquisition module comprise temperature sensor for measuring growth room's temperature information, for measure growth room's humidity information humidity sensor, for measure light conditions in growth room light sensor, for measuring the carbon dioxide sensor of gas concentration lwevel in growth room, temperature sensor, humidity sensor, light sensor, carbon dioxide sensor all connect master control system.The present invention can gather the information of temperature in growth room, humidity, illumination, gas concentration lwevel by information acquisition module, and send to master control system, make user can be understood environmental parameter in growth room in real time by calculator, to make adjustment to environmental parameter in time.
Strain culturing environment balance control system selected by Asparagus, also comprise an environmental parameter regulating system, environmental parameter regulating system comprises a humidifier adjusted for growth room's humidity, one is provided with for placing the bedstead of blake bottle in growth room, bedstead from bottom to top, at least be provided with six layers, the gas outlet of each humidifier is all communicated with Jun Gu growth room by least one humidification pipeline; The end of giving vent to anger of humidification pipeline is arranged between the top of bedstead and the second high level; Humidifier connects master control system.User can control the working condition of humidifier by calculator or master control system, and then the humidity in adjustment growth room.
Environmental parameter regulating system also comprises a new wind passage ventilated for growth room, the air inlet of new wind passage and extraneous UNICOM, the gas outlet of new wind passage and UNICOM of growth room, the gas outlet place of new wind passage is provided with a part sieve oxygenerator, and molecular-sieve oxygen generator connects master control system.User can control molecular-sieve oxygen generator by calculator or master control system, and then adjusts gas concentration lwevel by the oxygen concentration in adjustment growth room.
Environmental parameter regulating system also comprises an air-cooler freezed for growth room, and the gas outlet of air-cooler is positioned at growth room, and air-cooler connects master control system.User can control the working condition of air-cooler by calculator or master control system, and then the temperature in adjustment growth room.
Environmental parameter regulating system also comprises growth room's sun shield, and growth room's sun shield is positioned at the light inlet window place of growth room, and growth room's sun shield connects a sun shield governor motion, and sun shield governor motion connects master control system.User can control sun shield governor motion by calculator or master control system, and then the state of adjustment growth room sun shield, thus controls the logical light situation of growth room.
The temperature of growth room preferably 5 DEG C.When temperature is higher than 5.5 DEG C, calculator or master control system control air-cooler and start working, and temperature is declined, and when temperature is lower than 4.5 DEG C, calculator or master control system close air-cooler, and the growth of bacterium mushroom produces heat, makes temperature increase.
The humidity of growth room preferably 98%.When humidity higher than 99% time, calculator or master control system control deep bead governor motion and open deep bead, and new air is entered, thus reduce indoor humidity.When humidity lower than 97% time, calculator or master control system control to open humidifier, and humidity is increased.
The gas concentration lwevel of growth room is preferred 10000ppm.When humidity is higher than 12000ppm, calculator or master control system open molecular-sieve oxygen generator, thus increase indoor oxygen content, thus reduce indoor carbon dioxide content.When humidity is lower than 8000ppm, calculator or master control system closure molecule sieve oxygenerator, Growth of Flammulina Velutipes is breathed and gas concentration lwevel is increased.
Total light application time in the whole growth cycle of Asparagus preferably 40 hours, when light application time is constantly little more than 45, calculator or master control system control sun shield governor motion and close growth room's sun shield, make Asparagus avoid illumination.The mode of controlled light is adopt the mode of repeatedly irradiating in short-term, and repeatedly irradiation time controls in irradiation per hour once, and once irradiating is within 3.5 minutes; Also irradiation time can be controlled to irradiate once in per half an hour, each irradiation time controls within 1.8 minutes; Also irradiation time can be controlled to irradiate once at every 2 hours, each irradiation time controlled within 7 minutes.
A network interface established by calculator, and calculator connects computer network by network interface.The equipment of such distant place just can understand the ambient conditions in growth room by network, to make adjustment in time.
The signal output part of information acquisition module can connect master control system by a wireless communication module, preferably, and the signal output part wired connection wireless communication module of information acquisition module, wireless communication module wireless connections master control system.Such the present invention gathers the information of growth room's environmental parameter by information acquisition module, send to master control system wirelessly by wireless communication module, information acquisition module, wireless communication module, master control system, calculator and computer network form an Internet of things system.
Temperature sensor connects a temperature wireless transmitter module, and temperature sensor connects master control system by temperature wireless transmitter module.Humidity sensor connects a humidity wireless transmitter module, and humidity sensor connects master control system by humidity wireless transmitter module.Light sensor connects a light conditions wireless transmitter module, and light sensor connects master control system by light conditions wireless transmitter module.Carbon dioxide sensor connects a gas concentration lwevel wireless transmitter module, and carbon dioxide sensor connects master control system by gas concentration lwevel wireless transmitter module.
More than show and describe general principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (9)
1. strain culturing technique selected by Asparagus, comprises medium preparing, strain improvement, inoculation, growing environment regulation and control, it is characterized in that:
Described growing environment regulation and control comprise:
Control growth indoor temperature is 4.5 degrees Celsius ~ 5.5 degrees Celsius, humidity is 97% ~ 99%, the volume by volume concentration of carbonic acid gas is 8000PPM ~ 12000PPM;
The total light application time controlling the whole growth cycle of Asparagus is 38 hours ~ 42 hours;
During described growing environment regulation and control, strain culturing environment balance control system is selected to regulate and control by Asparagus, strain culturing environment balance control system selected by Asparagus, comprise an environmental parameter regulating system, described environmental parameter regulating system comprises a humidifier adjusted for growth room's humidity, and be provided with one in growth room for placing the bedstead of blake bottle, described bedstead from bottom to top, at least be provided with six layers, the gas outlet of each humidifier is all communicated with Jun Gu growth room by least one humidification pipeline; The end of giving vent to anger of described humidification pipeline is arranged between the top of described bedstead and the second high level;
Described humidifier connects a master control system, and described master control system connects a calculator;
When humidity higher than 99% time, calculator or master control system control deep bead governor motion and open deep bead, and new air is entered, thus reduce indoor humidity, when humidity lower than 97% time, calculator or master control system control to open humidifier, and humidity is increased.
2. strain culturing technique selected by Asparagus according to claim 1, it is characterized in that: when inoculating, in culture bottle, the moisture of medium controls at 66%-68%.
3. strain culturing technique selected by Asparagus according to claim 1, it is characterized in that: during strain improvement, in the Asparagus grown under growing environment according to claim 1, selection good bacterial strain mutually, is separated by tissue and cultivates, make its stability of characteristics, as bacterial classification.
4. strain culturing technique selected by Asparagus according to claim 2, it is characterized in that: the temperature of described growth room is 5 DEG C, humidity is 98%, carbonic acid gas volume by volume concentration is 10000ppm, total light application time in the whole growth cycle of Asparagus is 40 hours;
When inoculating, in culture bottle, the moisture of medium is 67%.
5. strain culturing technique selected by the Asparagus according to claim 1,2,3 or 4, it is characterized in that: strain culturing environment balance control system selected by Asparagus, also comprise a growth room of cultivating for Asparagus, be provided with the information acquisition module of a collection growth room environmental parameter in described growth room, the signal output part of described information acquisition module connects described master control system;
Described information acquisition module comprise temperature sensor for measuring growth room's temperature information, for measure growth room's humidity information humidity sensor, for measure light conditions in growth room light sensor, for measuring the carbon dioxide sensor of gas concentration lwevel in growth room, described temperature sensor, described humidity sensor, described light sensor, described carbon dioxide sensor all connect described master control system.
6. strain culturing technique selected by Asparagus according to claim 5, it is characterized in that: described environmental parameter regulating system also comprises a new wind passage ventilated for growth room, the air inlet of described new wind passage and extraneous UNICOM, the gas outlet of described new wind passage and UNICOM of growth room, the gas outlet place of described new wind passage is provided with a part sieve oxygenerator, and described molecular-sieve oxygen generator connects described master control system;
When gas concentration lwevel is higher than 12000ppm, calculator or master control system open molecular-sieve oxygen generator, thus increase indoor oxygen content, thus reduce indoor carbon dioxide content, when gas concentration lwevel is lower than 8000ppm, calculator or master control system closure molecule sieve oxygenerator, Growth of Flammulina Velutipes is breathed and gas concentration lwevel is increased.
7. strain culturing technique selected by Asparagus according to claim 6, it is characterized in that: described environmental parameter regulating system also comprises an air-cooler freezed for growth room, the gas outlet of described air-cooler is positioned at described growth room, and described air-cooler connects described master control system;
When temperature is higher than 5.5 DEG C, calculator or master control system control air-cooler and start working, and temperature is declined, and when temperature is lower than 4.5 DEG C, calculator or master control system close air-cooler, and the growth of bacterium mushroom produces heat, makes temperature increase.
8. strain culturing technique selected by Asparagus according to claim 7, it is characterized in that: described environmental parameter regulating system also comprises growth room's sun shield, described growth room sun shield is positioned at the light inlet window place of growth room, described growth room sun shield connects a sun shield governor motion, and described sun shield governor motion connects described master control system;
When light application time is constantly little more than 42, calculator or master control system control described sun shield governor motion and close growth room's sun shield, make Asparagus avoid illumination.
9. strain culturing technique selected by Asparagus according to claim 8, it is characterized in that: the mode of controlled light is adopt the mode of repeatedly irradiating in short-term, and repeatedly irradiation time controls in irradiation per hour once, and once irradiating is within 3.5 minutes;
Or irradiation time controlled to irradiate once in per half an hour, each irradiation time controls within 1.8 minutes;
Or irradiation time controlled to irradiate once at every 2 hours, each irradiation time controlled within 7 minutes.
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| CN105165400B (en) * | 2015-09-15 | 2018-07-27 | 上海雪榕生物科技股份有限公司 | The method of the quick fruiting of Pleurotus nebrodensis low temperature after-ripening |
| CN105165399A (en) * | 2015-09-15 | 2015-12-23 | 上海雪榕生物科技股份有限公司 | Method for adjusting production states of edible fungi through measurement of respiratory capacity of edible fungi |
| CN105165397A (en) * | 2015-09-15 | 2015-12-23 | 上海雪榕生物科技股份有限公司 | Method for measuring respiratory capacity of lentinula edodes |
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| CN101940126B (en) * | 2010-08-09 | 2012-02-29 | 镇江市丹徒区正东生态农业发展中心 | Fruiting shaping technology in golden mushroom industrial cultivation |
| CN102640662A (en) * | 2012-05-23 | 2012-08-22 | 李珏成 | Plateau flammulina velutipes culture method |
| CN102860220B (en) * | 2012-10-25 | 2014-02-19 | 广东星河生物科技股份有限公司 | Cultivation method of bottle planted needle mushroom |
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