CN105165397A - Method for measuring respiratory capacity of lentinula edodes - Google Patents
Method for measuring respiratory capacity of lentinula edodes Download PDFInfo
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- CN105165397A CN105165397A CN201510586087.XA CN201510586087A CN105165397A CN 105165397 A CN105165397 A CN 105165397A CN 201510586087 A CN201510586087 A CN 201510586087A CN 105165397 A CN105165397 A CN 105165397A
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Abstract
The invention relates to the technical field of lentinula edodes, in particular to a method for measuring the respiratory capacity of lentinula edodes. The method for measuring the respiratory capacity of lentinula edodes includes the steps that 1, a culture bottle in which lentinula edodes is placed is placed in a seal box; 2, a carbon dioxide measuring instrument and a timer are placed, and the carbon dioxide measuring instrument is started; 3, the timer begins to conduct timing while the carbon dioxide measuring instrument is started; 4, in the whole culture process, the respiratory capacity of lentinula edodes is regularly detected every day; 5, the change of the respiratory capacity of lentinula edodes in the whole growth process is obtained through calculation. The respiratory capacity of lentinula edodes is measured, the change of production process parameters is adjusted and controlled by acquiring the numerical values of the respirator capacity, the optimal requirements of mycelial growth can be met, and the optimal yield is obtained.
Description
Technical field
The present invention relates to mushroom technical field, particularly relate to mushroom pneumatometry.
Background technology
Mushroom, having another name called flower mushroom, Hericium erinaceus, mushroom, fragrant letter, fragrant bacterium, dried mushroom, fragrant wild rice, is the fruit body of Pleurotaceae plant mushroom.Mushroom is second-biggest-in-the-world mushroom, one of Ye Shi China special product, at the title have " mountain delicacy " among the people.It is the fungi of a kind of growth on timber.Delicious flavour, fragrance oozes people, nutritious.Mushroom is rich in Cobastab group, iron, potassium, provitamin D (changing into vitamin D after Exposure to Sunlight), taste are sweet, and property is put down.Cure mainly anorexia, weak breath is weak.
The title that mushroom have " king of mountain delicacy " is high protein, low-fat nutritional health food.Chinese dynasties physician all has famous discussion to mushroom.Modern medicine and alimentology deepen continuously research, and the medical value of mushroom is also constantly excavated.In mushroom, Quantitative Determination of Ergosterol is very high, effective to preventing and treating rickets; Lentinan (β ~ 1,3 glucans) can strengthen cell immunocompetent, thus the growth of inhibition cancer cell; Mushroom contains more than 40 kind of enzyme of six large enzymes, can correct human chitinase deficiency disease; Fatty institute fatty acids in mushroom, reduces blood fat to human body useful.
Mushroom--fungi queen.Mushroom, also known as mushroom, dried mushroom, is the Mycophyta of a kind of growth on timber.Because its taste is more fragrant, pleasant aroma, nutritious, not only rank on straw mushroom, flat mushroom, white mushroom, and have the reputation of " fungi queen ".
Current most of mushroom can carry out artificial cultivation, and mushroom growth process be unable to do without air, and it absorbs oxygen by respiration and discharges carbonic acid gas, and can embody the situation of its growth in any case.In air, the content of oxygen is about 21%, and the content of carbonic acid gas is 0.03% (300ppm).Too high CO2 concentration can affect the respiratory activity of mushroom, and the CO2 of high concentration suppresses mycelial growth.Therefore seem to the control of mushroom respiratory capacity and be even more important, when carbonic acid gas exceedes a certain amount of, mushroom can growth retardation or produce deformity, thus causes and yield poorly, and even atrophy is dead.
Summary of the invention
The object of the present invention is to provide a kind of assay method of mushroom respiratory capacity, to solve the problems of the technologies described above.
Technical problem solved by the invention can realize by the following technical solutions:
An assay method for mushroom respiratory capacity, is characterized in that, described assay method comprises:
Step one, puts into seal box by the culture bottle being placed with mushroom;
Step 2, puts into CO-2 analyser and timer; CO-2 analyser is started shooting;
Step 3, open CO-2 analyser simultaneously timer start timing;
Step 4, in whole incubation, every day, timing detected the respiratory capacity of mushroom;
Step 5, calculates the change of the whole process of growth respiratory capacity of this kind mushroom.
The present invention measures mushroom respiratory capacity, by obtaining the change of the numerical value regulation and control production process parameters of respiratory capacity, being convenient to the best demand meeting mycelial growth, obtaining optimum point of production.
Described culture bottle comprises a upper cover and a lower cover, and described upper cover comprises circular upper cover plate, has ring-type to the upper cover limit of downward-extension, have outer latch closure in the outside on upper cover limit below circular upper cover plate, described lower cover comprises circular lower cover, ring-type is had to the lower cover limit of downward-extension below circular lower cover, the inner becket be interlocked with cultivation bottleneck is had in the inside on lower cover limit, the outer ring body upwards extended and inner ring body is had on circular lower cover, there is the inner becket be interlocked with latch closure outside upper cover limit the inside of outer ring body, lower cover between outer ring body and inner ring body there are multiple ventilation holes of annular distribution, multiple ventilation breach of annular distribution are had on the upper edge of inner ring body, the lower cover at inner ring body center has reinforcement and multiple blow vent, this blow vent constitutes with ventilation breach and ventilation hole the scavenge duct communicated with the external world,
Reinforcement on described lower cover and blow vent are the blow vents of criss-crossing reinforcing ribs and four annular arrangements;
Described upper cover plate also comprises the anti-skidding salient point of multiple annular arrangement, and skid-proof point has spilehole.
The present invention is provided with some anti-skidding salient points upper covering, and culture bottle is not easy landing and damages bottle when stacking.Described ventilation breach and ventilation hole mutually staggered at circumferencial direction, define a mazy scavenge duct, between upper cover and lower cover, there is a cavity at the center of inner ring body, and this cavity can place filter pad, can effectively stop outside air to the pollution of medium.Upper cover and lower cover fasten when using, and can take apart, the cleaning of aspect lid portion inside narrow positions during cleaning.
Described CO-2 analyser is positioned at the inwall of seal box, and described CO-2 analyser connects a microprocessor system, the central axis of described culture bottle and the central axes of described seal box.
The present invention detects gas concentration lwevel more accurately by CO-2 analyser being placed on wall energy in seal box.
Described CO-2 analyser comprises the digital display screen of carbon dioxide sensor and display gas concentration lwevel, and described carbon dioxide sensor connects described microprocessor system, and described microprocessor system connects described digital display screen; Described carbon dioxide sensor is positioned at the inwall of culture bottle.
The present invention, by carbon dioxide sensor being placed on the inwall of culture bottle, can detecting mushroom more accurately and breathe the gas concentration lwevel produced, and digital display screen can be convenient to observe.
Described digital display screen connects a memory module, and described memory module stores dense carbon dioxide degrees of data; Described memory module connects a host computer.
The present invention is convenient to by memory module the data storing CO-2 analyser detection, can also, by data upload to host computer, be convenient to draw a diagram or figure.
Described memory module also connects a Resetting Switching.
The present invention carries out deletion by Resetting Switching to the data of memory module and resets, and resets, is convenient to use next time.
Also be provided with environment conditioning system in described seal box, described environment conditioning system comprises an attemperating unit;
Described attemperating unit comprises the information acquisition module of a collection seal box environment parameter, and the signal output part of described information acquisition module connects a master control system, and described master control system connects a calculator;
Described information acquisition module comprises the temperature sensor for measuring seal box temperature information.
Be convenient to detect the temperature variations in seal box.
Described temperature sensor is a radio temperature sensor, is provided with a wireless communication module in described temperature sensor, and described master control system connects a wireless receiving module matched with described wireless communication module;
Described temperature sensor is positioned at the inwall of seal box.
The change of sealing the temperature inside the box can be detected more accurately.
Described attemperating unit connects described master control system, and described master control system also connects a clock module.
By temperature sensor senses to signal give master control system, adjustment attemperating unit regulation and control situation is carried out to the temperature in seal box, make mushroom be in the temperature of suitable growth.
Described attemperating unit also comprises an air-cooler for freezing, and the gas outlet of described air-cooler is positioned at described seal box, and described air-cooler connects described master control system;
When temperature is higher than 5 DEG C ~ 10 DEG C, calculator or master control system control air-cooler and start working, and temperature is declined, and when temperature is lower than 1 DEG C ~ 3 DEG C, calculator or master control system close air-cooler, and mushroom growth produces heat, makes temperature increase.By these means can realize temperature from Row sum-equal matrix.
Described carbon dioxide sensor also connects described master control system, and described master control system also connects an illumination apparatus, and described illumination apparatus is positioned at the inwall of culture bottle, and the light emission direction of described illumination apparatus is towards mushroom.
When gas concentration lwevel is too high, master control system makes illumination apparatus open, and makes mushroom carry out photosynthesis absorbing carbon dioxide, increases oxygen, promotes mushroom growth.
Described illumination apparatus comprises LED light-emitting component, and described LED light-emitting component connects the electric energy output end of power storage module, and described illumination apparatus is provided with a shell, and the outer wall of described shell is provided with USB interface, and described USB interface is connected with the electrical energy inputs of power storage module.
The present invention provides illumination by LED light-emitting component, provides electric energy by USB interface to LED light-emitting component.
As a kind of scheme, when the gas concentration lwevel that carbon dioxide sensor detects is 9000ppm-13000ppm, described illumination apparatus is in holding state;
When the gas concentration lwevel that carbon dioxide sensor detects is 13000ppm-17000ppm, described illumination apparatus is opened, and light emission direction is towards mushroom;
When the gas concentration lwevel that carbon dioxide sensor detects is 5000ppm-9000ppm, described illumination apparatus reenters holding state.
Now gas concentration lwevel scope is the optimum scope in mushroom incubation; Ensure that while illumination, the temperature in seal box keeps stable.
Alternatively, when the gas concentration lwevel that carbon dioxide sensor detects is 1000ppm-13000ppm, described illumination apparatus is in holding state;
When the gas concentration lwevel that carbon dioxide sensor detects is 13000ppm-16000ppm, described illumination apparatus is opened, and light emission direction is towards mushroom;
When the gas concentration lwevel that carbon dioxide sensor detects is 7000ppm-10000ppm, described illumination apparatus reenters holding state.
Optimum gas concentration lwevel when now gas concentration lwevel is mushroom fruiting.
Accompanying drawing explanation
Fig. 1 is flow chart of the present invention.
Embodiment
The technological means realized to make the present invention, creation characteristic, reaching object and effect is easy to understand, setting forth the present invention further below in conjunction with concrete diagram.
With reference to Fig. 1, a kind of assay method of mushroom respiratory capacity, assay method comprises: step one, and the culture bottle being placed with mushroom is put into seal box; Step 2, puts into CO-2 analyser and timer; CO-2 analyser is started shooting; Step 3, open CO-2 analyser simultaneously timer start timing; Step 4, in whole incubation, every day, timing detected the respiratory capacity of mushroom; Step 5, calculates the change of the whole process of growth respiratory capacity of this kind mushroom.The present invention measures mushroom respiratory capacity, by obtaining the change of the numerical value regulation and control production process parameters of respiratory capacity, being convenient to the best demand meeting mycelial growth, obtaining optimum point of production.Culture bottle comprises a upper cover and a lower cover, upper cover comprise circular upper cover plate, below circular upper cover plate, have ring-type to the upper cover limit of downward-extension, have outer latch closure in the outside on upper cover limit, lower cover comprise circular lower cover, ring-type is had to the lower cover limit of downward-extension below circular lower cover, the inner becket be interlocked with cultivation bottleneck is had in the inside on lower cover limit, the outer ring body upwards extended and inner ring body is had on circular lower cover, there is the inner becket be interlocked with latch closure outside upper cover limit the inside of outer ring body, lower cover between outer ring body and inner ring body there are multiple ventilation holes of annular distribution, multiple ventilation breach of annular distribution are had on the upper edge of inner ring body, the lower cover at inner ring body center has reinforcement and multiple blow vent, this blow vent constitutes with ventilation breach and ventilation hole the scavenge duct communicated with the external world, reinforcement on lower cover and blow vent are the blow vents of criss-crossing reinforcing ribs and four annular arrangements, upper cover plate also comprise the anti-skidding salient point of multiple annular arrangement, skid-proof point has spilehole.
The present invention is provided with some anti-skidding salient points upper covering, and culture bottle is not easy landing and damages bottle when stacking.Ventilation breach and ventilation hole mutually staggered at circumferencial direction, define a mazy scavenge duct, between upper cover and lower cover, there is a cavity at the center of inner ring body, and this cavity can place filter pad, can effectively stop outside air to the pollution of medium.Upper cover and lower cover fasten when using, and can take apart, the cleaning of aspect lid portion inside narrow positions during cleaning.CO-2 analyser is positioned at the inwall of seal box, and CO-2 analyser connects a microprocessor system, the central axis of culture bottle and the central axes of seal box.The present invention detects gas concentration lwevel more accurately by CO-2 analyser being placed on wall energy in seal box.CO-2 analyser comprises the digital display screen of carbon dioxide sensor and display gas concentration lwevel, and carbon dioxide sensor connects microprocessor system, and microprocessor system connects digital display screen; Carbon dioxide sensor is positioned at the inwall of culture bottle.The present invention, by carbon dioxide sensor being placed on the inwall of culture bottle, can detecting mushroom more accurately and breathe the gas concentration lwevel produced, and digital display screen can be convenient to observe.
Digital display screen connects a memory module, and memory module stores dense carbon dioxide degrees of data; Memory module connects a host computer.The present invention is convenient to by memory module the data storing CO-2 analyser detection, can also, by data upload to host computer, be convenient to draw a diagram or figure.Memory module also connects a Resetting Switching.The present invention carries out deletion by Resetting Switching to the data of memory module and resets, and resets, is convenient to use next time.Also be provided with environment conditioning system in seal box, environment conditioning system comprises an attemperating unit; Attemperating unit comprises the information acquisition module of a collection seal box environment parameter, and the signal output part of information acquisition module connects a master control system, and master control system connects a calculator; Information acquisition module comprises the temperature sensor for measuring seal box temperature information.Be convenient to detect the temperature variations in seal box.Temperature sensor is a radio temperature sensor, is provided with a wireless communication module in temperature sensor, and master control system connects a wireless receiving module matched with wireless communication module; Temperature sensor is positioned at the inwall of seal box.The change of sealing the temperature inside the box can be detected more accurately.
Attemperating unit connects master control system, and master control system also connects a clock module.By temperature sensor senses to signal give master control system, adjustment attemperating unit regulation and control situation is carried out to the temperature in seal box, make mushroom be in the temperature of suitable growth.Attemperating unit also comprises an air-cooler for freezing, and the gas outlet of air-cooler is positioned at seal box, and air-cooler connects master control system; When temperature is higher than 5 DEG C ~ 10 DEG C, calculator or master control system control air-cooler and start working, and temperature is declined, and when temperature is lower than 1 DEG C ~ 3 DEG C, calculator or master control system close air-cooler, and mushroom growth produces heat, makes temperature increase.By these means can realize temperature from Row sum-equal matrix.Carbon dioxide sensor also connects master control system, and master control system also connects an illumination apparatus, and illumination apparatus is positioned at the inwall of culture bottle, and the light emission direction of illumination apparatus is towards mushroom.When gas concentration lwevel is too high, master control system makes illumination apparatus open, and makes mushroom carry out photosynthesis absorbing carbon dioxide, increases oxygen, promotes mushroom growth.Illumination apparatus comprises LED light-emitting component, and LED light-emitting component connects the electric energy output end of power storage module, and illumination apparatus is provided with a shell, and the outer wall of shell is provided with USB interface, and USB interface is connected with the electrical energy inputs of power storage module.The present invention provides illumination by LED light-emitting component, provides electric energy by USB interface to LED light-emitting component.
As a kind of scheme, when the gas concentration lwevel that carbon dioxide sensor detects is 9000ppm-13000ppm, illumination apparatus is in holding state; When the gas concentration lwevel that carbon dioxide sensor detects is 13000ppm-17000ppm, illumination apparatus is opened, and light emission direction is towards mushroom; When the gas concentration lwevel that carbon dioxide sensor detects is 5000ppm-9000ppm, illumination apparatus reenters holding state.Now gas concentration lwevel scope is the optimum scope in mushroom incubation; Ensure that while illumination, the temperature in seal box keeps stable.Alternatively, when the gas concentration lwevel that carbon dioxide sensor detects is 1000ppm-13000ppm, illumination apparatus is in holding state; When the gas concentration lwevel that carbon dioxide sensor detects is 13000ppm-16000ppm, illumination apparatus is opened, and light emission direction is towards mushroom; When the gas concentration lwevel that carbon dioxide sensor detects is 7000ppm-10000ppm, illumination apparatus reenters holding state.Optimum gas concentration lwevel when now gas concentration lwevel is mushroom fruiting.
More than show and describe general principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (10)
1. an assay method for mushroom respiratory capacity, is characterized in that, described assay method comprises:
Step one, puts into seal box by the culture bottle being placed with mushroom;
Step 2, puts into CO-2 analyser and timer; CO-2 analyser is started shooting;
Step 3, open CO-2 analyser simultaneously timer start timing;
Step 4, in whole incubation, every day, timing detected the respiratory capacity of mushroom;
Step 5, calculates the change of the whole process of growth respiratory capacity of this kind mushroom.
2. the assay method of a kind of mushroom respiratory capacity according to claim 1, it is characterized in that: described culture bottle comprises a upper cover and a lower cover, described upper cover comprises circular upper cover plate, below circular upper cover plate, there is ring-type to the upper cover limit of downward-extension, have outer latch closure in the outside on upper cover limit, described lower cover comprises circular lower cover, ring-type is had to the lower cover limit of downward-extension below circular lower cover, the inner becket be interlocked with cultivation bottleneck is had in the inside on lower cover limit, the outer ring body upwards extended and inner ring body is had on circular lower cover, there is the inner becket be interlocked with latch closure outside upper cover limit the inside of outer ring body, lower cover between outer ring body and inner ring body there are multiple ventilation holes of annular distribution, multiple ventilation breach of annular distribution are had on the upper edge of inner ring body, the lower cover at inner ring body center has reinforcement and multiple blow vent, this blow vent constitutes with ventilation breach and ventilation hole the scavenge duct communicated with the external world,
Reinforcement on described lower cover and blow vent are the blow vents of criss-crossing reinforcing ribs and four annular arrangements;
Described upper cover plate also comprises the anti-skidding salient point of multiple annular arrangement, and skid-proof point has spilehole.
3. the assay method of a kind of mushroom respiratory capacity according to claim 1, it is characterized in that: described CO-2 analyser is positioned at the inwall of seal box, described CO-2 analyser connects a microprocessor system, the central axis of described culture bottle and the central axes of described seal box;
Described CO-2 analyser comprises the digital display screen of carbon dioxide sensor and display gas concentration lwevel, and described carbon dioxide sensor connects described microprocessor system, and described microprocessor system connects described digital display screen; Described carbon dioxide sensor is positioned at the inwall of culture bottle.
4. the assay method of a kind of mushroom respiratory capacity according to claim 3, is characterized in that: described digital display screen connects a memory module, and described memory module stores dense carbon dioxide degrees of data; Described memory module connects a host computer; Described memory module also connects a Resetting Switching.
5. the assay method of a kind of mushroom respiratory capacity according to claim 3, is characterized in that: be also provided with environment conditioning system in described seal box, and described environment conditioning system comprises an attemperating unit;
Described attemperating unit comprises the information acquisition module of a collection seal box environment parameter, and the signal output part of described information acquisition module connects a master control system, and described master control system connects a calculator;
Described information acquisition module comprises the temperature sensor for measuring seal box temperature information;
Described attemperating unit connects described master control system, and described master control system also connects a clock module.
6. the assay method of a kind of mushroom respiratory capacity according to claim 5, it is characterized in that: described temperature sensor is a radio temperature sensor, be provided with a wireless communication module in described temperature sensor, described master control system connects a wireless receiving module matched with described wireless communication module;
Described temperature sensor is positioned at the inwall of seal box.
7. the assay method of a kind of mushroom respiratory capacity according to claim 5, it is characterized in that: described attemperating unit also comprises an air-cooler for freezing, the gas outlet of described air-cooler is positioned at described seal box, and described air-cooler connects described master control system;
When temperature is higher than 5 DEG C ~ 10 DEG C, calculator or master control system control air-cooler and start working, and temperature is declined, and when temperature is lower than 1 DEG C ~ 3 DEG C, calculator or master control system close air-cooler, and mushroom growth produces heat, makes temperature increase.
8. the assay method of a kind of mushroom respiratory capacity according to claim 3, it is characterized in that: described carbon dioxide sensor also connects described master control system, described master control system also connects an illumination apparatus, described illumination apparatus is positioned at the inwall of culture bottle, and the light emission direction of described illumination apparatus is towards mushroom.
9. the assay method of a kind of mushroom respiratory capacity according to claim 8, it is characterized in that: described illumination apparatus comprises LED light-emitting component, described LED light-emitting component connects the electric energy output end of power storage module, described illumination apparatus is provided with a shell, the outer wall of described shell is provided with USB interface, and described USB interface is connected with the electrical energy inputs of power storage module.
10. the assay method of a kind of mushroom respiratory capacity according to claim 8, is characterized in that: when the gas concentration lwevel that carbon dioxide sensor detects is 1000ppm-13000ppm, described illumination apparatus is in holding state;
When the gas concentration lwevel that carbon dioxide sensor detects is 13000ppm-16000ppm, described illumination apparatus is opened, and light emission direction is towards mushroom;
When the gas concentration lwevel that carbon dioxide sensor detects is 7000ppm-10000ppm, described illumination apparatus reenters holding state.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114994263A (en) * | 2022-07-15 | 2022-09-02 | 华中农业大学 | Fruit and vegetable respiration physiology measuring device |
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