CN103842501B - 病毒载体纯化系统 - Google Patents
病毒载体纯化系统 Download PDFInfo
- Publication number
- CN103842501B CN103842501B CN201280049032.XA CN201280049032A CN103842501B CN 103842501 B CN103842501 B CN 103842501B CN 201280049032 A CN201280049032 A CN 201280049032A CN 103842501 B CN103842501 B CN 103842501B
- Authority
- CN
- China
- Prior art keywords
- cell surface
- surface marker
- vector
- purification
- viral
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
- C12N7/02—Recovery or purification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
- C12N2740/13051—Methods of production or purification of viral material
- C12N2740/13052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
- C12N2740/15051—Methods of production or purification of viral material
- C12N2740/15052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16051—Methods of production or purification of viral material
- C12N2740/16052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP11183937.9 | 2011-10-05 | ||
| EP11183937 | 2011-10-05 | ||
| PCT/EP2012/069713 WO2013050523A1 (en) | 2011-10-05 | 2012-10-05 | Viral vectors purification system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103842501A CN103842501A (zh) | 2014-06-04 |
| CN103842501B true CN103842501B (zh) | 2017-12-08 |
Family
ID=47071247
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201280049032.XA Expired - Fee Related CN103842501B (zh) | 2011-10-05 | 2012-10-05 | 病毒载体纯化系统 |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US20140248695A1 (https=) |
| EP (1) | EP2764094A1 (https=) |
| JP (1) | JP2014528729A (https=) |
| KR (1) | KR20140068259A (https=) |
| CN (1) | CN103842501B (https=) |
| AU (1) | AU2012320480A1 (https=) |
| BR (1) | BR112014008225A2 (https=) |
| CA (1) | CA2848939A1 (https=) |
| IL (1) | IL231876A0 (https=) |
| IN (1) | IN2014CN03368A (https=) |
| RU (1) | RU2607044C2 (https=) |
| WO (1) | WO2013050523A1 (https=) |
| ZA (1) | ZA201401481B (https=) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2480677T3 (en) | 2010-09-02 | 2015-06-15 | Molmed Spa | Semi-stable production of lentiviral vectors |
| GB201310664D0 (en) | 2013-06-14 | 2013-07-31 | Mars Inc | Feline taste receptors |
| BR112018070256A2 (pt) | 2016-03-31 | 2019-01-29 | Spark Therapeutics, Inc. | processo de fabricação de raav totalmente escalável à base de colunas |
| JP2025143019A (ja) * | 2024-03-18 | 2025-10-01 | アイポア株式会社 | ウイルスベクターの検査方法及び機械学習システム |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5654183A (en) * | 1992-07-27 | 1997-08-05 | California Institute Of Technology | Genetically engineered mammalian neural crest stem cells |
| IT1261847B (it) | 1993-09-01 | 1996-06-03 | San Romanello Centro Fond | Metodo per marcare cellule eucariote. |
| EP0781343B1 (de) * | 1994-09-08 | 2003-07-02 | Vision 7 GmbH | Retrovirale vektorhybride und deren verwendung zum gentransfer |
| US5837520A (en) * | 1995-03-07 | 1998-11-17 | Canji, Inc. | Method of purification of viral vectors |
| EP1284287A4 (en) * | 2000-05-10 | 2004-10-13 | Mitsubishi Pharma Corp | METHOD FOR PRODUCING A VIRUS VECTOR |
| IL154987A0 (en) * | 2000-09-22 | 2003-10-31 | Virxsys | Conditionally replicating viral vectors and their use |
| GB0224442D0 (en) * | 2002-10-21 | 2002-11-27 | Molmed Spa | A delivery system |
| GB0413702D0 (en) | 2004-06-18 | 2004-07-21 | Molmed Spa | Thymidine kinase |
| JP3968383B2 (ja) * | 2004-09-01 | 2007-08-29 | 株式会社日本メディカル総研 | 血液細胞の新規分類法ならびにそれを利用したテイラーメード治療および予防 |
| EP1739180A1 (en) * | 2005-06-27 | 2007-01-03 | Max-Delbrück-Centrum für Molekulare Medizin (MDC) | T cell line-based packaging cell line for the production of retroviruses by enriching of CD3 expressing cells |
| JP4921083B2 (ja) * | 2005-09-13 | 2012-04-18 | タカラバイオ株式会社 | レトロウィルス産生用無血清培地 |
| WO2011060218A1 (en) * | 2009-11-13 | 2011-05-19 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Modulated programmed death ligand-1 |
| GB0920775D0 (en) * | 2009-11-26 | 2010-01-13 | King S College | Cells |
| DK2480677T3 (en) | 2010-09-02 | 2015-06-15 | Molmed Spa | Semi-stable production of lentiviral vectors |
| WO2012028680A1 (en) | 2010-09-02 | 2012-03-08 | Molmed Spa | Semi-stable production of lentiviral vectors |
-
2012
- 2012-10-05 AU AU2012320480A patent/AU2012320480A1/en not_active Abandoned
- 2012-10-05 CN CN201280049032.XA patent/CN103842501B/zh not_active Expired - Fee Related
- 2012-10-05 EP EP12775654.2A patent/EP2764094A1/en not_active Withdrawn
- 2012-10-05 RU RU2014117466A patent/RU2607044C2/ru not_active IP Right Cessation
- 2012-10-05 JP JP2014533916A patent/JP2014528729A/ja not_active Ceased
- 2012-10-05 BR BR112014008225A patent/BR112014008225A2/pt not_active IP Right Cessation
- 2012-10-05 WO PCT/EP2012/069713 patent/WO2013050523A1/en not_active Ceased
- 2012-10-05 KR KR1020147011842A patent/KR20140068259A/ko not_active Ceased
- 2012-10-05 US US14/349,531 patent/US20140248695A1/en not_active Abandoned
- 2012-10-05 IN IN3368CHN2014 patent/IN2014CN03368A/en unknown
- 2012-10-05 CA CA2848939A patent/CA2848939A1/en not_active Abandoned
-
2014
- 2014-02-26 ZA ZA2014/01481A patent/ZA201401481B/en unknown
- 2014-04-02 IL IL231876A patent/IL231876A0/en unknown
Non-Patent Citations (3)
| Title |
|---|
| Cellular Compartments of Human Immunodeficiency Virus Type 1 Replication In Vivo: Determination by Presence of Virion-Associated Host Proteins and Impact of Opportunistic Infection;STEPHEN D. LAWN等;《Journal of Virology》;20000131;第74卷(第1期);139-145页 * |
| Selective Immunoaffinity-Based Enrichment of CD34+ Cells Transduced with Retroviral Vectors Containing an Intracytoplasmatically Truncated Version of the Human Low-Affinity Nerve Growth Factor Receptor (ΔLNGFR) Gene;BORIS FEHSE等;《Human Gene Therapy》;19971010;第8卷;第1815页摘要 * |
| Tagging Retrovirus Vectors with a Metal Binding Peptide and One-Step Purification by Immobilized Metal Affinity Chromatography;Kaiming Ye等;《Journal of Virology》;20040930;第78卷(第18期);第9821页左栏第4段、右栏第1、4-5段 * |
Also Published As
| Publication number | Publication date |
|---|---|
| US20140248695A1 (en) | 2014-09-04 |
| IL231876A0 (en) | 2014-05-28 |
| AU2012320480A1 (en) | 2014-04-24 |
| WO2013050523A1 (en) | 2013-04-11 |
| JP2014528729A (ja) | 2014-10-30 |
| RU2014117466A (ru) | 2015-11-10 |
| KR20140068259A (ko) | 2014-06-05 |
| ZA201401481B (en) | 2015-04-29 |
| IN2014CN03368A (https=) | 2015-07-03 |
| EP2764094A1 (en) | 2014-08-13 |
| RU2607044C2 (ru) | 2017-01-10 |
| NZ623092A (en) | 2016-07-29 |
| BR112014008225A2 (pt) | 2017-04-11 |
| CA2848939A1 (en) | 2013-04-11 |
| CN103842501A (zh) | 2014-06-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171208 Termination date: 20181005 |