IN2014CN03368A - - Google Patents

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Publication number
IN2014CN03368A
IN2014CN03368A IN3368CHN2014A IN2014CN03368A IN 2014CN03368 A IN2014CN03368 A IN 2014CN03368A IN 3368CHN2014 A IN3368CHN2014 A IN 3368CHN2014A IN 2014CN03368 A IN2014CN03368 A IN 2014CN03368A
Authority
IN
India
Prior art keywords
surface marker
cell surface
purification
vectors
viral
Prior art date
Application number
Inventor
Chiara Bovolenta
Anna Stornaiuolo
Original Assignee
Molmed Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Molmed Spa filed Critical Molmed Spa
Publication of IN2014CN03368A publication Critical patent/IN2014CN03368A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13051—Methods of production or purification of viral material
    • C12N2740/13052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15051—Methods of production or purification of viral material
    • C12N2740/15052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16051—Methods of production or purification of viral material
    • C12N2740/16052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present invention relates to a new method for purification of viral vectors particularly those belonging to the Retroviridae family which is based on the expression in the packaging cell line that produced such vectors of an exogenous gene encoding a cell surface marker. The incorporation of the cell surface marker in the viral envelope of the vector allows purification with immunological methods.
IN3368CHN2014 2011-10-05 2012-10-05 IN2014CN03368A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11183937 2011-10-05
PCT/EP2012/069713 WO2013050523A1 (en) 2011-10-05 2012-10-05 Viral vectors purification system

Publications (1)

Publication Number Publication Date
IN2014CN03368A true IN2014CN03368A (en) 2015-07-03

Family

ID=47071247

Family Applications (1)

Application Number Title Priority Date Filing Date
IN3368CHN2014 IN2014CN03368A (en) 2011-10-05 2012-10-05

Country Status (13)

Country Link
US (1) US20140248695A1 (en)
EP (1) EP2764094A1 (en)
JP (1) JP2014528729A (en)
KR (1) KR20140068259A (en)
CN (1) CN103842501B (en)
AU (1) AU2012320480A1 (en)
BR (1) BR112014008225A2 (en)
CA (1) CA2848939A1 (en)
IL (1) IL231876A0 (en)
IN (1) IN2014CN03368A (en)
RU (1) RU2607044C2 (en)
WO (1) WO2013050523A1 (en)
ZA (1) ZA201401481B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103228790B (en) 2010-09-02 2015-08-19 莫尔梅德股份有限公司 The stably manufactured of lentiviral vectors
GB201310664D0 (en) 2013-06-14 2013-07-31 Mars Inc Feline taste receptors
RU2754467C2 (en) 2016-03-31 2021-09-02 Спарк Терапьютикс, Инк. COMPLETELY SCALABLE METHOD FOR PRODUCING rAAV BASED ON COLUMNS

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5654183A (en) * 1992-07-27 1997-08-05 California Institute Of Technology Genetically engineered mammalian neural crest stem cells
IT1261847B (en) 1993-09-01 1996-06-03 San Romanello Centro Fond METHOD FOR MARKING EUCARIOT CELLS.
JPH09511405A (en) * 1994-09-08 1997-11-18 ベーリンガー マンハイム ゲゼルシャフト ミット ベシュレンクテル ハフツング Retroviral vector hybrid and its use for gene transfer
US5837520A (en) * 1995-03-07 1998-11-17 Canji, Inc. Method of purification of viral vectors
WO2001085928A1 (en) * 2000-05-10 2001-11-15 Mitsubishi Pharma Corporation Method of preparing virus vector
RU2301260C2 (en) * 2000-09-22 2007-06-20 Вирэкссис Корпорейшн Viral vectors with condition-dependent replication and their using
GB0224442D0 (en) * 2002-10-21 2002-11-27 Molmed Spa A delivery system
GB0413702D0 (en) 2004-06-18 2004-07-21 Molmed Spa Thymidine kinase
JP3968383B2 (en) * 2004-09-01 2007-08-29 株式会社日本メディカル総研 Novel classification method of blood cells and tailor-made treatment and prevention using it
EP1739180A1 (en) * 2005-06-27 2007-01-03 Max-Delbrück-Centrum für Molekulare Medizin (MDC) T cell line-based packaging cell line for the production of retroviruses by enriching of CD3 expressing cells
JP4921083B2 (en) * 2005-09-13 2012-04-18 タカラバイオ株式会社 Serum-free medium for retrovirus production
US20120251514A1 (en) * 2009-11-13 2012-10-04 University Health Network Modulated programmed death ligand-1
GB0920775D0 (en) * 2009-11-26 2010-01-13 King S College Cells
CA2809447C (en) 2010-09-02 2019-12-03 Molmed S.P.A. Semi-stable production of lentiviral vectors
CN103228790B (en) 2010-09-02 2015-08-19 莫尔梅德股份有限公司 The stably manufactured of lentiviral vectors

Also Published As

Publication number Publication date
IL231876A0 (en) 2014-05-28
RU2607044C2 (en) 2017-01-10
JP2014528729A (en) 2014-10-30
KR20140068259A (en) 2014-06-05
RU2014117466A (en) 2015-11-10
US20140248695A1 (en) 2014-09-04
WO2013050523A1 (en) 2013-04-11
ZA201401481B (en) 2015-04-29
AU2012320480A1 (en) 2014-04-24
EP2764094A1 (en) 2014-08-13
NZ623092A (en) 2016-07-29
BR112014008225A2 (en) 2017-04-11
CN103842501B (en) 2017-12-08
CN103842501A (en) 2014-06-04
CA2848939A1 (en) 2013-04-11

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