CN103834699A - 使用改进量的氯和钾在微藻类中产生高水平的dha - Google Patents

使用改进量的氯和钾在微藻类中产生高水平的dha Download PDF

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Publication number
CN103834699A
CN103834699A CN201410126418.7A CN201410126418A CN103834699A CN 103834699 A CN103834699 A CN 103834699A CN 201410126418 A CN201410126418 A CN 201410126418A CN 103834699 A CN103834699 A CN 103834699A
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dha
less
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保罗.W.贝伦斯
约翰.M.汤普森
柯克.阿普特
约瑟夫.W.法伊弗第三
詹姆斯.P.温
詹姆斯.C.利普迈耶
贾奥亚德.菲克塔里
乔恩.汉森
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DSM IP Assets BV
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/02—Algae
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/10—Production of fats or fatty oils from raw materials by extracting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Biomedical Technology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Public Health (AREA)
  • Medical Informatics (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201410126418.7A 2003-10-02 2004-10-01 使用改进量的氯和钾在微藻类中产生高水平的dha Pending CN103834699A (zh)

Applications Claiming Priority (2)

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US50850503P 2003-10-02 2003-10-02
US60/508,505 2003-10-02

Related Parent Applications (1)

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CN2004800356858A Division CN1890376B (zh) 2003-10-02 2004-10-01 使用改进量的氯和钾在微藻类中产生高水平的dha

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CN103834699A true CN103834699A (zh) 2014-06-04

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CN201410126418.7A Pending CN103834699A (zh) 2003-10-02 2004-10-01 使用改进量的氯和钾在微藻类中产生高水平的dha
CN200810166137.9A Expired - Lifetime CN101386873B (zh) 2003-10-02 2004-10-01 使用改进量的氯和钾在微藻类中产生高水平的dha
CN2004800356858A Expired - Lifetime CN1890376B (zh) 2003-10-02 2004-10-01 使用改进量的氯和钾在微藻类中产生高水平的dha

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CN200810166137.9A Expired - Lifetime CN101386873B (zh) 2003-10-02 2004-10-01 使用改进量的氯和钾在微藻类中产生高水平的dha
CN2004800356858A Expired - Lifetime CN1890376B (zh) 2003-10-02 2004-10-01 使用改进量的氯和钾在微藻类中产生高水平的dha

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US (17) US7163811B2 (enExample)
EP (2) EP2759599A3 (enExample)
JP (4) JP4733043B2 (enExample)
KR (6) KR20140119198A (enExample)
CN (3) CN103834699A (enExample)
AU (2) AU2004280575B8 (enExample)
CA (2) CA2540617A1 (enExample)
DK (1) DK1702073T3 (enExample)
ES (1) ES2454196T3 (enExample)
HK (1) HK1199289A1 (enExample)
MX (4) MX351381B (enExample)
NO (1) NO20061916L (enExample)
WO (1) WO2005035775A1 (enExample)

Families Citing this family (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1252324T3 (da) 2000-01-19 2010-12-20 Martek Biosciences Corp Solventfri ekstraktionsfremgangsmåde
CA2540617A1 (en) 2003-10-02 2005-04-21 Martek Biosciences Corporation Production of high levels of dha in microalgae using modified amounts of chloride and potassium
WO2007120801A2 (en) * 2006-04-13 2007-10-25 Nereus Pharmaceuticals, Inc. Fermentation method
EP1847543A1 (de) 2006-04-19 2007-10-24 Boehringer Ingelheim Pharma GmbH & Co. KG Dihydrothienopyrimidine zur Behandlung von entzündlichen Erkrankungen
WO2008049512A1 (en) * 2006-10-27 2008-05-02 Lonza Ag Production of omega-3 fatty acids in microflora of thraustochytriales using modified media
EP2205609B1 (de) * 2007-10-19 2017-03-29 Boehringer Ingelheim International GmbH Heterocyclus-substituierte piperazino-dihydrothienopyrimidine
BRPI0818006B8 (pt) * 2007-10-19 2021-05-25 Boehringer Ingelheim Int compostos de piperidino-di-hidrotienopirimidinas substituídas, uso dos mesmos e formulações farmacêuticas
JP5563466B2 (ja) 2007-10-19 2014-07-30 ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング 新規ピペラジノ−ジヒドロチエノピリミジン誘導体
US20100239712A1 (en) 2008-10-14 2010-09-23 Solazyme, Inc. Food Compositions of Microalgal Biomass
US8809037B2 (en) 2008-10-24 2014-08-19 Bioprocessh20 Llc Systems, apparatuses and methods for treating wastewater
EP2194138A1 (en) * 2008-12-08 2010-06-09 Marenutrica GmbH Compositions containing docosahexaenoic acid and method for its production
CN102333880A (zh) 2009-02-25 2012-01-25 V.B.医疗私人有限公司 发酵生产二十二碳六烯酸的改进方法
US8207363B2 (en) * 2009-03-19 2012-06-26 Martek Biosciences Corporation Thraustochytrids, fatty acid compositions, and methods of making and uses thereof
CA2758479C (en) 2009-04-14 2019-09-10 Solazyme, Inc. Novel microalgal food compositions
AU2010343305B2 (en) 2010-01-19 2015-06-11 Dsm Ip Assets B.V. Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof
ES2784774T3 (es) 2010-04-14 2020-09-30 Corbion Biotech Inc Composiciones alimenticias de harina de microalgas ricas en lípidos
US8940520B2 (en) 2010-05-20 2015-01-27 Pond Biofuels Inc. Process for growing biomass by modulating inputs to reaction zone based on changes to exhaust supply
US11512278B2 (en) 2010-05-20 2022-11-29 Pond Technologies Inc. Biomass production
US20120156669A1 (en) 2010-05-20 2012-06-21 Pond Biofuels Inc. Biomass Production
US8889400B2 (en) 2010-05-20 2014-11-18 Pond Biofuels Inc. Diluting exhaust gas being supplied to bioreactor
US8969067B2 (en) 2010-05-20 2015-03-03 Pond Biofuels Inc. Process for growing biomass by modulating supply of gas to reaction zone
JP5911479B2 (ja) 2010-06-01 2016-04-27 ディーエスエム アイピー アセッツ ビー．ブイ． 細胞からの脂質の抽出およびそれに由来する生産物
MY163938A (en) 2010-06-14 2017-11-15 Io-Mega Holding Corp Method for the production of algae derived oils
US20120276633A1 (en) 2011-04-27 2012-11-01 Pond Biofuels Inc. Supplying treated exhaust gases for effecting growth of phototrophic biomass
US8795589B1 (en) 2011-04-29 2014-08-05 Cortec Corporation Bio-based volatile corrosion inhibitors
TWI582235B (zh) 2011-07-21 2017-05-11 Ｄｓｍ智慧財產有限公司 生產二十碳五烯酸之微生物、脂肪酸組成物及其製作方法與用途
US9802954B2 (en) 2011-08-24 2017-10-31 Boehringer Ingelheim International Gmbh Piperidino-dihydrothienopyrimidine sulfoxides and their use for treating COPD and asthma
US20130059866A1 (en) 2011-08-24 2013-03-07 Boehringer Ingelheim International Gmbh Novel piperidino-dihydrothienopyrimidine sulfoxides and their use for treating copd and asthma
CN103773687B (zh) * 2012-10-23 2019-07-12 中国石油化工股份有限公司 一种微藻的培养方法
US9534261B2 (en) 2012-10-24 2017-01-03 Pond Biofuels Inc. Recovering off-gas from photobioreactor
JP2014223024A (ja) * 2013-05-15 2014-12-04 日本電信電話株式会社 微細藻類の培養方法および培養装置
CN103627640B (zh) * 2013-08-10 2018-03-02 青岛海智源生命科技有限公司 一种培养裂殖壶菌的低氯培养基及用该培养基产生dha的方法
CN103468575B (zh) * 2013-09-18 2015-05-06 武汉轻工大学 一株高产dha的寇氏隐甲藻突变株及发酵方法和应用
KR101494649B1 (ko) * 2013-12-03 2015-02-23 한국에너지기술연구원 미생물로부터 차아염소산을 이용한 바이오오일 추출 방법
WO2015095690A2 (en) 2013-12-20 2015-06-25 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
NZ721409A (en) 2013-12-20 2022-10-28 Dsm Ip Assets Bv Processes for obtaining microbial oil from microbial cells
JP6705581B2 (ja) 2013-12-20 2020-06-03 ディーエスエム アイピー アセッツ ビー．ブイ．Ｄｓｍ Ｉｐ Ａｓｓｅｔｓ Ｂ．Ｖ． 微生物細胞から微生物油を入手するための方法
CA2934490C (en) 2013-12-20 2022-05-03 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
CA2934506C (en) 2013-12-20 2022-05-03 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
FR3019559B1 (fr) * 2014-04-03 2018-01-05 Fermentalg Procede de culture des microalgues du genre aurantiochytrium dans un milieu de culture a teneur reduite en sodium et en chlorure pour la production de dha
CA2953060C (en) 2014-05-22 2022-09-20 MARA Renewables Corporation Methods of oil production in microorganisms
US20150352034A1 (en) 2014-06-08 2015-12-10 Solazyme, Inc. Personal Care Products Containing Microalgae or Extracts Thereof
FR3025215A1 (fr) * 2014-08-27 2016-03-04 Fermentalg Nouveau procede de culture de microalgues
WO2016100681A1 (en) 2014-12-17 2016-06-23 Solazyme, Inc. Microalgal derived cleaners and surfactants
US9181523B1 (en) 2014-12-29 2015-11-10 Heliae Development Llc Method of treating bacterial contamination in a microalgae culture with pH shock
DK3274430T3 (da) 2015-03-24 2022-10-03 Corbion Biotech Inc Mikroalgesammensætninger og deres anvendelser
EP3445167B1 (en) * 2016-04-18 2021-01-20 Planktonic AS Cryopreservation of juvenile stages of barnacles
US11946017B2 (en) 2016-07-13 2024-04-02 Evonik Operations Gmbh Method of separating lipids from a lysed lipids containing biomass
US10773978B2 (en) 2016-08-19 2020-09-15 Noblegen, Inc. Methods and uses of dissolved organic material fractions for binding metal ions
EP3562925B1 (en) 2016-12-27 2021-03-10 Evonik Operations GmbH Method of isolating lipids from a lipids containing biomass
EP3606663A4 (en) 2017-04-07 2021-01-27 Noblegen Inc. PROCESSES AND USES OF ENCAPSULATED EXUDATES AND EUGLENA BIOMASS
EP3470502A1 (en) 2017-10-13 2019-04-17 Evonik Degussa GmbH Method of separating lipids from a lysed lipids containing biomass
EP3527664A1 (en) 2018-02-15 2019-08-21 Evonik Degussa GmbH Method of isolating lipids from a lipids containing biomass
WO2019219396A1 (en) 2018-05-15 2019-11-21 Evonik Operations Gmbh Method of isolating lipids from a lysed lipids containing biomass by emulsion inversion
DK3794097T3 (da) 2018-05-15 2024-12-16 Evonik Operations Gmbh Fremgangsmåde til isolering af lipider fra en lipid-indeholdende biomasse ved hjælp af hydrofob silica
KR102455795B1 (ko) * 2020-01-17 2022-10-17 고려대학교 산학협력단 지질 및 오메가-3 함량 증진을 위한 미세조류 배양 방법
EP4469588A1 (en) * 2022-01-25 2024-12-04 DSM IP Assets B.V. Media refinement and nutrient feeding approaches to increase polyunsaturated fatty acid production

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5130242A (en) 1988-09-07 1992-07-14 Phycotech, Inc. Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids
US5340742A (en) 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US5407957A (en) * 1990-02-13 1995-04-18 Martek Corporation Production of docosahexaenoic acid by dinoflagellates
US6410281B1 (en) * 1992-07-10 2002-06-25 Omegatech, Inc. Reducing corrosion in a fermentor by providing sodium with a non-chloride sodium salt
JPH0775556A (ja) 1993-09-09 1995-03-20 Nisshin Oil Mills Ltd:The ドコサヘキサエン酸含有藻類の培養法
JP3506740B2 (ja) * 1993-09-09 2004-03-15 日清オイリオ株式会社 ドコサヘキサエン酸含有藻類の培養方法
JPH0787959A (ja) * 1993-09-24 1995-04-04 Kawasaki Steel Corp 海洋性微細藻類の培養方法
JPH08294384A (ja) * 1995-04-27 1996-11-12 Kawasaki Steel Corp 海洋性微細藻類のシード培養方法
PT935667E (pt) 1996-07-23 2007-02-28 Nagase Chemtex Corp Processo para a preparação de áçido docosa-hexaenóico e de áçido docosapentaenóico
ES2446982T3 (es) * 1996-12-27 2014-03-11 Suntory Holdings Limited Medios para cultivar microorganismos y método para producir ácidos grasos insaturados o lípidos que los contienen
JP4283351B2 (ja) * 1998-08-27 2009-06-24 サントリー酒類株式会社 新規な油脂組成物の製造方法および用途
KR19990046733A (ko) * 1999-04-20 1999-07-05 류성구 미생물슈도모나스에의한도코사핵사노인산(ｄｈａ)의제조방법
GB9916537D0 (en) 1999-07-14 1999-09-15 Univ Hull Culture of microorganisms for the synthesis of a polyunsaturated fatty acid
DK1252324T3 (da) 2000-01-19 2010-12-20 Martek Biosciences Corp Solventfri ekstraktionsfremgangsmåde
IL150770A0 (en) 2000-01-28 2003-02-12 Omegatech Inc Enhanced production of lipids containing polyenoic fatty acids by high density cultures of eukaryotic microbes in fermentors
ES2336075T3 (es) 2000-04-12 2010-04-08 Gea Westfalia Separator Gmbh Metodo para el funcionamiento de materias primas nativas que contienen aceite y lipidos polares utilizando alcohol y centrifugacion.
CA2540617A1 (en) 2003-10-02 2005-04-21 Martek Biosciences Corporation Production of high levels of dha in microalgae using modified amounts of chloride and potassium

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
王永华，等: "隐甲藻发酵产DHA最佳无机盐浓度的确定", 《中国油脂》 *

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US20120071677A1 (en) 2012-03-22
US20080032353A1 (en) 2008-02-07
US20080032354A1 (en) 2008-02-07
JP5466120B2 (ja) 2014-04-09
US7824892B2 (en) 2010-11-02
MX304474B (enExample) 2012-10-22
HK1089482A1 (en) 2006-12-01
US8008050B2 (en) 2011-08-30
US7745183B2 (en) 2010-06-29
KR20130041366A (ko) 2013-04-24
US7871809B2 (en) 2011-01-18
WO2005035775A1 (en) 2005-04-21
EP2759599A3 (en) 2014-12-10
KR20140006109A (ko) 2014-01-15
NO20061916L (no) 2006-06-12
AU2004280575B8 (en) 2010-05-13
AU2004280575A1 (en) 2005-04-21
AU2010202691B2 (en) 2013-09-12
JP2011036261A (ja) 2011-02-24
US20080032355A1 (en) 2008-02-07
US20060100279A1 (en) 2006-05-11
CA2801065C (en) 2020-04-28
US20080032359A1 (en) 2008-02-07
JP5940194B2 (ja) 2016-06-29
US9834799B2 (en) 2017-12-05
CN1890376A (zh) 2007-01-03
US7252979B2 (en) 2007-08-07
US7163811B2 (en) 2007-01-16
CN1890376B (zh) 2012-06-13
JP2015130886A (ja) 2015-07-23
US20130150604A1 (en) 2013-06-13
MX351381B (es) 2017-10-12
US20080044866A1 (en) 2008-02-21
CA2540617A1 (en) 2005-04-21
EP1702073A1 (en) 2006-09-20
JP5813061B2 (ja) 2015-11-17
MX338455B (es) 2016-04-18
JP2013212126A (ja) 2013-10-17
EP2759599A2 (en) 2014-07-30
US20130158280A1 (en) 2013-06-20
US8663953B2 (en) 2014-03-04
US20080032356A1 (en) 2008-02-07
US8669090B2 (en) 2014-03-11
EP1702073A4 (en) 2011-08-24
JP4733043B2 (ja) 2011-07-27
MX271416B (es) 2009-10-30
KR20110033283A (ko) 2011-03-30
US20080032358A1 (en) 2008-02-07
CA2801065A1 (en) 2005-04-21
US20080032352A1 (en) 2008-02-07
ES2454196T3 (es) 2014-04-09
CN101386873A (zh) 2009-03-18
CN101386873B (zh) 2014-05-07
DK1702073T3 (da) 2014-04-07
US7829129B2 (en) 2010-11-09
KR101761164B1 (ko) 2017-07-25
US20050112736A1 (en) 2005-05-26
US20080044865A1 (en) 2008-02-21
US20080032357A1 (en) 2008-02-07
KR20150125731A (ko) 2015-11-09
AU2010202691A1 (en) 2010-07-15
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US20140142331A1 (en) 2014-05-22
MXPA06003467A (es) 2006-06-20
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US9249434B2 (en) 2016-02-02
US20160108443A1 (en) 2016-04-21
HK1199289A1 (en) 2015-06-26
AU2004280575B2 (en) 2010-04-22
EP1702073B1 (en) 2014-01-01
KR101295390B1 (ko) 2013-09-17
KR20060097009A (ko) 2006-09-13
KR20140119198A (ko) 2014-10-08

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