CN103829097A - Feeding attractant for juvenile procambarus clarkii as well as preparation method and application of feeding attractant - Google Patents
Feeding attractant for juvenile procambarus clarkii as well as preparation method and application of feeding attractant Download PDFInfo
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- CN103829097A CN103829097A CN201410092186.8A CN201410092186A CN103829097A CN 103829097 A CN103829097 A CN 103829097A CN 201410092186 A CN201410092186 A CN 201410092186A CN 103829097 A CN103829097 A CN 103829097A
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Abstract
The invention discloses a feeding attractant for juvenile procambarus clarkii as well as a preparation method and application of the feed attractant. The feeding attractant comprises the following components in parts by weight: 30-40 parts of lycine, 15-25 parts of kanirin, 15-20 parts of dimethyl-beta-propiothetin and 20-30 of allicin. The preparation method of the feeding attractant comprises the following steps: weighing the components according to the weight parts and then mixing uniformly. The raw materials of the feeding attractant is stable in source and can be taken from a wide range, the production process is simple, the cost is low, and the effect is obvious; from the food intake and digestion mechanism of crustacean, the feeding attractant improves the food intake rate of procambarus clarkia bodies and reduces the waste of baits, simultaneously, the activity of digestive enzymes of the intestines and pancreases of the juvenile procambarus clarkii is improved to promote the growth of the procambarus clarkii.
Description
Technical field
The invention belongs to aquaculture field, relate to a kind of Procambius clarkii juvenile prawn phagostimulant and its preparation method and application.
Background technology
Procambius clarkii (Procambarus clarkii) is commonly called as " cray ", belongs to Arthropoda, Crustachia, is most important crayfist cultivation kind in the world today, and its output accounts for 70%-80% of whole huge legendary turtle shrimp output.Introduced after China from 1981, because of its delicious meat, be nutritiously subject to consumer's favor, be now distributed widely in the ground such as Jiangsu, Anhui, Hubei, Zhejiang and Shanghai, become China's important economic aquaculture object at present.Along with the continuous expansion of cultivation scale and the raising of intensive degree, Procambius clarkii---especially juvenile prawn artifical compound feed in period demand constantly expands.Therefore, in order to optimize feed formula, to reduce feed cost, increasing plant protein source substitute is used, and often has that palatability is poor, the problem of feed waste but add plant protein source in feed.Therefore, must develop effective Procambius clarkii juvenile prawn phagostimulant.
Phagostimulant, cites approvingly again lure, Appetizer or palatability additive, is a kind of phagostimulant non-nutritional feed additive.Phagostimulant can produce and stimulate the sense of smell of aquatic livestock, the sense of taste and vision etc., in bait, add the phagostimulant aquatic livestock of throwing something and feeding, can improve on the one hand palatability, strengthen animal appetite, improve animal feed intake and promote digesting and assimilating and utilizing etc. of feed, on the other hand, also reduced surplus bait in water, alleviated residual bait and degenerated the pollution to water quality.Study and show simultaneously, phagostimulant can also improve the digestive function of some aquatic livestock, improves protease in its enteron aisle and pancreas, lipase and diastatic activity, and promotion animal digests and assimilates feed, promotes growth; In addition be often used as, the also nutritious effect of tool of material of phagostimulant.But the shortcomings such as a lot of phagostimulants exist that cost is not low in the market, poor stability, food calling effect is not high, trophic level is general and specific aim is poor, the phagostimulant that therefore exploitation is targetedly, effective and cost is low becomes study hotspot,
At present more existing a small amount of uses in aquaculture about phagostimulants such as betaine, TMAO (TMAO), dimethyl-β-DMPT (DMPT) and allicins, but about the compatibility use between these several persons, have not been reported.Therefore, develop a kind of safe, efficient phagostimulant for Procambius clarkii juvenile prawn, significant to Procambius clarkii healthy aquaculture and sustainable development.
Summary of the invention
The object of this invention is to provide a kind of Procambius clarkii juvenile prawn phagostimulant and preparation method thereof, promote the Procambius clarkii juvenile prawn artificial granular mixed feed of ingesting with this, reduce production cost, maintain the sustainable development of Procambius clarkii juvenile prawn aquaculture.
A kind of Procambius clarkii juvenile prawn phagostimulant, it contains by weight: betaine 30～40 weight portions, TMAO (TMAO) 15～25 weight portions, dimethyl-β-DMPT (DMPT) 15～20 weight portions, allicin 20～30 weight portions.
A preparation method for Procambius clarkii juvenile prawn phagostimulant, prepares by following steps:
1. take by weight: betaine 30～35 weight portions, TMAO (TMAO) 15～25 weight portions, dimethyl-β-DMPT (DMPT) 15～20 weight portions, allicin 20～30 weight portions;
2. each component is pulverized through micronizer, crossed after 120 object screen clothes, in mixer, fully mix, make described composite feeding promoting agent.
Compared with prior art, the invention has the advantages that:
1. from present conventional aquaculture phagostimulant, to filter out betaine, TMAO, dimethyl-β-DMPT and allicin composite according to specific proportioning in the present invention, use in Procambius clarkii juvenile prawn feed as feed addictive, addition (percetage by weight) is only 0.5～1.5%, measure littlely, successful is better than one-component and by the composite composition of other proportionings;
2. show through juvenile prawn verification experimental verification, obviously promote ingesting of Procambius clarkii juvenile prawn with phagostimulant of the present invention, promote growth, improve the nutrition of shrimp body, raising digestive enzyme activity, reduces residual bait, is conducive to protection of the environment;
3. the present invention's raw materials enjoy stable sources used, cheap, production technology is simple.
The specific embodiment
Embodiment 1
Get betaine 30 weight portions, TMAO20 weight portion, DMPT20 weight portion, allicin 30 weight portions; Above-mentioned each component is pulverized through micronizer, crossed after 120 object screen clothes, in mixer, fully mix, make composite feeding promoting agent.
Embodiment 2
Growth test: Procambius clarkii is provided by the Lu Kou of Jiangsu Fresh Water Aquatic Product Inst. base.Health, specification, the basically identical shrimp of weight are selected in test, the individual just about 4.31g of counterpoise.Random packet, be provided with control group A (basal diet of throwing something and feeding), control group B(adds 1.0% betaine in basal diet), control group C(adds 1.0%TMAO in basal diet), control group D(adds 1.0%DMPT in basal diet), control group E(adds 1.0% allicin in basal diet), control group F(adds 0.5% betaine+0.5% allicin at basal diet), control group G(adds 0.5%TMAO+0.5% allicin at basal diet), control group H(is day two kinds of interpolation 0.5%DMPT+0.5% allicins on basis), control group I(adds 0.2% betaine+0.2%TMAO+0.1%DMPT+0.5% allicin at basal diet), control group J(adds 0.5% betaine+0.2%TMAO+0.2%DMPT+0.1% allicin at basal diet) and test group (adding 1.0% composite feeding promoting agent in basal diet), establish 3 parallel tests for every group, every parallel group of 11 tail test shrimps, totally 15 box for breeding (volume is 160L).Shrimp domestication after 7 days, throw something and feed 10 groups of control group daily rations and 1.0% composite feeding promoting agent 30 days, measure the indexs such as its growth, digestive ferment.
Table 1 basal diet formula
Note: compound premix (every kg feed): vitamin e1 50mg, vitamin K5 0mg, vitamin B1 80mg, vitamin B2 50mg, nicotinic acid 150mg, pantothenic acid 150mg, vitamin B6 50mg, biotin 1mg, cobalamin 0.02mg, folic acid 10mg, vitamin C 300IU, retinol1 0000IU, calciferol 000IU. copper sulphate 2.0g, ferric sulfate 25g, zinc sulfate 22g, manganese sulfate 7g, sodium selenite 0.04g, KI 0.026g, cobalt chloride 0.1g.
Result shows: compared with control group A, test group has significantly improved: rate of body weight gain 43.92%, grazing rate 34.51%; Significantly reduce feed coefficient 21.03%.Compared with control group B, test group has significantly improved rate of body weight gain 24.38%, grazing rate 17.87%; Significantly reduce feed coefficient 13.00%.Compared with control group C, test group has significantly improved: rate of body weight gain 25.58%, grazing rate 25.53%; Significantly reduce feed coefficient 12.00%.Compared with control group D, test group has significantly improved: rate of body weight gain 34.01%, grazing rate 28.30%; Significantly reduce feed coefficient 18.52%.Compared with control group E, test group has significantly improved: rate of body weight gain 20.76%, grazing rate 16.98%; Significantly reduce feed coefficient 12.00%.Compared with control group F, test group has significantly improved: rate of body weight gain 18.86%.Compared with control group G, test group has significantly improved: rate of body weight gain 16.83%, grazing rate 15.73%.Compared with control group H, test group has significantly improved: rate of body weight gain 19.77%, grazing rate 15.58%; Significantly reduce feed coefficient 11.49%.Compared with control group I, test group has significantly improved: rate of body weight gain 14.69%, grazing rate 10.22%.Compared with control group J, test group has significantly improved: rate of body weight gain 16.68%, grazing rate 12.42%.
Therefore, phagostimulant of the present invention can significantly improve rate of body weight gain and the grazing rate of Procambius clarkii juvenile prawn than each control group (A-I), significantly reduced feed coefficient; Thereby more can promote shrimp bulk-growth, reduce residual bait, be conducive to environmental protection.
Table 2
Result shows: compared with control group A, test group has significantly improved: enteron aisle lipase 47.86%, enteron aisle amylase 75.24%, pancreas trypsase 118.82%.Compared with control group B, test group has significantly improved: enteron aisle lipase 25.56%, enteron aisle amylase 2 0.52%, pancreas trypsase 78.92%.Compared with control group C, test group has significantly improved: enteron aisle lipase 64.35%, pancreas trypsase 70.74%.Compared with control group D, test group has significantly improved: enteron aisle amylase 2 5.34%, pancreas trypsase 61.45%.With control group E ratio, test group has significantly improved: enteron aisle amylase 67.74%, pancreas trypsase 15.65%.Compared with control group F, test group has significantly improved: enteron aisle amylase 41.72%, pancreas trypsase 43.44%.Compared with control group G, test group has significantly improved: enteron aisle lipase 25.88%, pancreas trypsase 49.34%.Compared with control group H, test group has significantly improved: enteron aisle amylase 43.32%, pancreas trypsase 22.73%.With control group I ratio, test group has significantly improved: enteron aisle amylase 2 9.56%. and control group J ratio, test group has significantly improved: pancreas trypsase 33.40%.
Therefore, phagostimulant of the present invention can significantly improve the digestive enzyme activity of Procambius clarkii juvenile prawn enteron aisle and pancreas than each control group, effectively utilize bait, promotes shrimp bulk-growth, reduces excretion, reduces water environment pollution.
Table 3
Embodiment 3
Get betaine 35 weight portions, TMAO22 weight portion, DMPT15 weight portion, allicin 28 weight portions; Above-mentioned each component is pulverized through micronizer, crossed after 120 object screen clothes, in mixer, fully mix, make composite feeding promoting agent.
Embodiment 4
Get betaine 40 weight portions, TMAO15 weight portion, DMPT15 weight portion, allicin 30 weight portions; Above-mentioned each component is pulverized through micronizer, crossed after 120 object screen clothes, in mixer, fully mix, make composite feeding promoting agent.
Embodiment 5
Get betaine 33 weight portions, TMAO17 weight portion, DMPT20 weight portion, allicin 30 weight portions; Above-mentioned each component is pulverized through micronizer, crossed after 120 object screen clothes, in mixer, fully mix, make composite feeding promoting agent.
Embodiment 6
Get betaine 37 weight portions, TMAO25 weight portion, DMPT18 weight portion, allicin 20 weight portions; Above-mentioned each component is pulverized through micronizer, crossed after 120 object screen clothes, in mixer, fully mix, make composite feeding promoting agent.
Embodiment 7
Get betaine 40 weight portions, TMAO22 weight portion, DMPT15 weight portion, allicin 23 weight portions; Above-mentioned each component is pulverized through micronizer, crossed after 120 object screen clothes, in mixer, fully mix, make composite feeding promoting agent.
Embodiment 8
Get betaine 35 weight portions, TMAO24 weight portion, DMPT15 weight portion, allicin 26 weight portions; Above-mentioned each component is pulverized through micronizer, crossed after 120 object screen clothes, in mixer, fully mix, make composite feeding promoting agent.
Embodiment 9
According to the growth test method in embodiment 2, the control group basal diet of throwing something and feeding, each test group experimental group (A～F) is measured respectively the composite feeding promoting agent of throwing something and feeding containing preparation in embodiment 3～8, and measures the indexs such as each group of Procambius clarkii juvenile prawn growth, digestive ferment, the results are shown in Table 4～5.
Table 4
Table 5
Claims (4)
1. a Procambius clarkii juvenile prawn phagostimulant, is characterized in that composed of the following components: betaine 30～40 weight portions, TMAO TMAO15～25 weight portion, dimethyl-β-DMPT DMPT15～20 weight portion, allicin 20～30 weight portions.
2. a preparation method for Procambius clarkii juvenile prawn phagostimulant, is characterized in that comprising the steps:
1. take by weight: betaine 30～35 weight portions, TMAO TMAO15～25 weight portion, dimethyl-β-DMPT DMPT15～20 weight portion, allicin 20～30 weight portions;
2. each component is pulverized through micronizer, crossed after 120 object screen clothes, in mixer, fully mix, make described composite feeding promoting agent.
3. the preparation method of Procambius clarkii juvenile prawn phagostimulant according to claim 2, is characterized in that: described each component will be dried, then pulverizes through micronizer, crosses after 120 object screen clothes, in mixer, fully mixes and makes.
4. composite feeding promoting agent according to claim 2, adds gained in conventional freshwater crayfish feed stuff to according to the amount of 0.5wt%～1.5wt%.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104543593A (en) * | 2015-01-28 | 2015-04-29 | 阳东县澳琦饲料添加剂有限公司 | Fish and shrimp attractant and preparation method thereof |
| CN105231095A (en) * | 2015-11-06 | 2016-01-13 | 中国水产科学研究院淡水渔业研究中心 | Composite phagostimulant for juvenile freshwater shrimps, preparation method of composite phagostimulant and freshwater shrimp compound feed |
| CN107996882A (en) * | 2017-12-13 | 2018-05-08 | 无锡华诺威动物保健品有限公司 | One seed shrimp crab special feed premix and its preparation method and application |
| CN109601704A (en) * | 2019-01-31 | 2019-04-12 | 盐城工学院 | A kind of lobster nutrient fodder and preparation method thereof |
| CN112913989A (en) * | 2021-03-19 | 2021-06-08 | 上海市农业科学院 | Macaca crayfish phagostimulant and preparation method and application thereof |
| CN115316558A (en) * | 2022-08-11 | 2022-11-11 | 盐城工学院 | Formula of efficient special feed phagostimulant for procambarus clarkii |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104543593A (en) * | 2015-01-28 | 2015-04-29 | 阳东县澳琦饲料添加剂有限公司 | Fish and shrimp attractant and preparation method thereof |
| CN105231095A (en) * | 2015-11-06 | 2016-01-13 | 中国水产科学研究院淡水渔业研究中心 | Composite phagostimulant for juvenile freshwater shrimps, preparation method of composite phagostimulant and freshwater shrimp compound feed |
| CN107996882A (en) * | 2017-12-13 | 2018-05-08 | 无锡华诺威动物保健品有限公司 | One seed shrimp crab special feed premix and its preparation method and application |
| CN109601704A (en) * | 2019-01-31 | 2019-04-12 | 盐城工学院 | A kind of lobster nutrient fodder and preparation method thereof |
| CN112913989A (en) * | 2021-03-19 | 2021-06-08 | 上海市农业科学院 | Macaca crayfish phagostimulant and preparation method and application thereof |
| CN115316558A (en) * | 2022-08-11 | 2022-11-11 | 盐城工学院 | Formula of efficient special feed phagostimulant for procambarus clarkii |
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