CN103828733B - Animal stimulating system - Google Patents

Animal stimulating system Download PDF

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Publication number
CN103828733B
CN103828733B CN201410040134.6A CN201410040134A CN103828733B CN 103828733 B CN103828733 B CN 103828733B CN 201410040134 A CN201410040134 A CN 201410040134A CN 103828733 B CN103828733 B CN 103828733B
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China
Prior art keywords
animal
control unit
opening
test casing
analysis
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CN201410040134.6A
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CN103828733A (en
Inventor
赵旭东
刘苏
王秀春
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Institute of Biophysics of CAS
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Institute of Biophysics of CAS
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Priority to CN201410040134.6A priority Critical patent/CN103828733B/en
Priority to CN201510292450.7A priority patent/CN104823872B/en
Publication of CN103828733A publication Critical patent/CN103828733A/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K15/00—Devices for taming animals, e.g. nose-rings or hobbles; Devices for overturning animals in general; Training or exercising equipment; Covering boxes
    • A01K15/02—Training or exercising equipment, e.g. mazes or labyrinths for animals ; Electric shock devices ; Toys specially adapted for animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K15/00—Devices for taming animals, e.g. nose-rings or hobbles; Devices for overturning animals in general; Training or exercising equipment; Covering boxes
    • A01K15/02—Training or exercising equipment, e.g. mazes or labyrinths for animals ; Electric shock devices ; Toys specially adapted for animals
    • A01K15/029—Electric or similar shock devices, e.g. prods
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K29/00—Other apparatus for animal husbandry
    • A01K29/005—Monitoring or measuring activity, e.g. detecting heat or mating

Abstract

The invention provides animal stimulating system and comprise at least one test casing, stimulating apparatus, the casing light source being arranged on described test casing top, harvester, the Analysis Control Unit being provided with analysis and Control software, heater and refrigerating plant.The stimulating apparatus that the present invention also provides animal stimulating method to comprise by being arranged on test casing stimulates animal; Gathered by the characteristic information of harvester to animal being arranged on test casing; Analyzed by the experimental data of Analysis Control Unit to the video acquisition device received, audio collecting device and temperature collecting device collection, send stimulation instructions to light stimulus module, Sound stimulat module, electrical stimulation module, send to food supply device and whether carry out food supply instruction and send temperature control instruction to heater or refrigerating plant.Animal stimulating system provided by the invention and method thereof, function is complicated, can reduce the zooperal interference of outer bound pair, improves the accuracy of experimental result.

Description

Animal stimulating system
Technical field
The present invention relates to zoopery field, particularly a kind of animal stimulating system.
Background technology
Existing animal experiment device capable is due to relatively simple for structure, function ratio is more single, complicated zoopery can not be done, need the many factors of human intervention (such as, manually remove feeding), artificial needs experimentally need to carry out artificial interference at the scene, because the interference in the external world can impact animal, thus impact the result accuracy of experiment.In addition, existing experiment condition, because experimenter needs in experimental field observation experiment result, also limit the activity space of experimenter.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of animal stimulating system.
For solving the problems of the technologies described above, the invention provides a kind of animal stimulating system, comprise at least one test casing, stimulating apparatus, the casing light source being arranged on described test casing top, harvester, the Analysis Control Unit being provided with analysis and Control software, heater and refrigerating plant; Described test casing is made up of thick PVC base and stainless steel faceplate, glass plate is provided with in described test casing, it is interior and parallel with the base of described test casing that described glass plate is arranged on described test casing, and form accommodation space between the base of described glass plate and described test casing; One jiao of described glass plate is provided with opening; Described opening part is provided with opening opening and closing device, for when carrying out feeding animal, opens described opening, when feeding animal is complete, closes described opening; Described test casing is divided into two intervals by the fence that movably shuttles back and forth; The described fence that shuttles back and forth is arranged in the middle part of described glass plate, and is provided with and carries out for animal the door that shuttles back and forth that shuttles back and forth; Described test box external body is provided with sound proof box, and described test casing top or sidewall are provided with the fan providing ventilation function; Described stimulating apparatus comprises light stimulus module, Sound stimulat module and electrical stimulation module; Described light stimulus module comprises stimulating light source and control circuit; Shuttle back and forth on fence or the top being arranged on described test casing or sidewall described in described stimulating light source is arranged on, described control circuit is electrically connected with described Analysis Control Unit, and the control command for receiving described Analysis Control Unit controls the opening and closing of described stimulating light source intensity of illumination, light application time and/or light source; Described Sound stimulat module installation, in described test casing, comprises audio-frequency power amplifier and sound source; Shuttle back and forth on fence or the top being arranged on described test casing or sidewall described in described sound source is arranged on, and be electrically connected with described Analysis Control Unit and described audio-frequency power amplifier, the control command for receiving described Analysis Control Unit controls the frequency of described sound, amplitude, duration and/or decibel; Described electrical stimulation module comprises electric stimulating instrument and exposed copper cash; It is external that described electric stimulating instrument is arranged on test box, and exposed copper cash is laid on the side of glass plate, and the output line of described electric stimulating instrument is electrically connected with exposed copper cash by the hole being located at test casing; Described harvester comprises camera head, speed acquisition device, temperature collecting device and voice collection device; Described camera head is separately positioned in described test casing top and described accommodation space, wherein, the camera head being arranged on described test casing top is for gathering the behavioural characteristic of animal and aspectual character thereof, the video data of collection for gathering the footprint of animal and aspectual character thereof, and is sent to described Analysis Control Unit by camera head in real time that be arranged on described test casing accommodation space inner bottom part; Described camera head is connected with described Analysis Control Unit by video card; Described voice collection device is connected with described Analysis Control Unit by interface circuit card, for gathering noise audio frequency and/or animal sounds, and the voice data of collection is sent to described Analysis Control Unit in real time; Described electrical stimulation module is connected with described Analysis Control Unit by interface circuit card; Described speed acquisition device is connected with described Analysis Control Unit by interface circuit card, for gathering the speed of animal, and the speed data of collection is sent to described Analysis Control Unit in real time; Described temperature collecting device for gathering the temperature in described test casing, and is provided with the Analysis Control Unit of analysis and Control software described in the temperature of collection being sent in real time;
Described Analysis Control Unit is used for the analysis according to the experimental data gathered the described video acquisition device received, audio collecting device and described temperature collecting device, send light stimulus instruction to described light stimulus module, send Sound stimulat instruction to described Sound stimulat module, send electro photoluminescence instruction to described electrical stimulation module, send to described food supply device and whether carry out food supply instruction, send temperature control instruction to described heater or described refrigerating plant, and send animal dead alarm;
Described food supply device is arranged on described accommodation space and is positioned at the below of the opening of described glass plate, and the control instruction for sending according to described Analysis Control Unit controls to carry out feeding to animal; Wherein, described food supply device comprises pallet, expansion link, motor, telescopic jig arm and microprocessor; Described microprocessor is connected with described Analysis Control Unit by interface circuit card, and the control instruction for sending according to described Analysis Control Unit controls expansion link described in motor driving and stretches, thus is convenient to feeding animal; Described pallet is square, and centre is provided with dividing plate, for food and moisture being separated; Described jig arm is connected with described microprocessor, after animal has reached food meal requirement, under described microprocessor-based control, by animal folder from described pallet;
The described control instruction for sending according to described Analysis Control Unit controls to carry out feeding to animal and comprises: analyzed the animal character information that described harvester gathers by described Analysis Control Unit, analyze and whether meet feeding requirement, when animal meets feeding requirement, control instruction is sent to described microprocessor, stretched by expansion link described in described Microprocessor S3C44B0X motor driving, control described opening by opening opening and closing device to open simultaneously, described pallet is delivered to described opening part, and parallel with the glass plate of opening part; When animal does not meet feeding requirement, control instruction is sent to described microprocessor, stretched by expansion link described in described Microprocessor S3C44B0X motor driving, described pallet is reduced to below described opening, control described opening by opening opening and closing device simultaneously and close.
Further, described Analysis Control Unit comprises the database server of the database and control software design be provided with for storing data and is provided with the mobile terminal of analysis software;
Described database server, receive the data message of described harvester collection and receive the control instruction of described mobile terminal by the transmission of wireless or internet, and control described harvester, stimulating apparatus, heater, refrigerating plant and food supply device according to described control instruction, and the data message of collection is sent to described mobile terminal;
Described mobile terminal, the data message that database server described in Real-time Obtaining obtains, in real time by wireless or internet to described database server sending controling instruction, and the data that described database server gathers to be analyzed the behavioural information of animal or electrophysiology information.
Further, described mobile terminal comprises data acquisition module, obtains the data message of animal under different stimulated from described database server; Analysis module, analyzes the data message of the animal obtained from described database server under different stimulated; The animal footprint information that data message analyze of the described animal to obtaining from described database server under different stimulated comprises described database server obtains is analyzed, and analyzes the animal footprint under different stimulated, and sorts out; Described animal footprint information comprises touchdown time, hang time, step-length, pressure, left and right pin spacing, front and back footprint note spacing and step sequence; And/or the animal that described database server obtains is analyzed under different stimulations, analyze under different stimulated, animal is to the reaction mean value of the duration of the reaction of response latency of the reaction amplitude peak stimulated, stimulation, stimulation, the speed of stimulation, the sound frequency of stimulation and stimulation; And/or judge whether animal meets feeding requirement according to the characteristic information of animal; And/or whether analyzing animal there is dead symptom; And/or according to the temperature data gathered, judge whether the temperature of testing in casing meets experiment needs; Control instruction sending module, the result analyzed according to described analysis module is to described database server sending controling instruction; Alarm module, when dead symptom appears in animal, sends warning message to user, to change laboratory animal in time.
Further, described opening opening and closing device comprises microprocessor, square glass, motor, rope and mechanical arm; Described mechanical arm is fixedly connected with described square glass; Described square glass and described opening slitless connection; Described motor is connected with described microprocessor and described rope respectively, for receiving described microprocessor-based control order, controlling described rope and stretching, fallen or put down described square glass, thus having realized the opening and closing of described opening; Described motor is fixed on the sidewall of described test box; Described motor is fixed on the sidewall of test casing; Described microprocessor is connected with Analysis Control Unit.
Further, four limits of described square glass are ramped shaped, and four limits of described opening are ramped shaped, thus realize the slitless connection of square glass and described opening, facilitate square glass can realize the disengaging with described opening smoothly simultaneously.
Further, describedly by described Analysis Control Unit, the animal character information that described harvester gathers to be analyzed, analyze and whether meet feeding and requires to include: the sign information of the animal of camera head collection is analyzed; The sign information of described animal comprises the size in animal belly region; When the size in described animal belly region is less than threshold value, judges that animal is in starvation, meet feeding requirement; When the size in described animal belly region is greater than threshold value, judges that animal is in fed to appetite state, do not meet feeding requirement.
Further, describedly by described Analysis Control Unit, the animal character information that described harvester gathers to be analyzed, analyze and whether meet feeding and requires to include: the speed of the animal of speed acquisition device collection and cry are analyzed; When the speed of described animal and/or cry exceed threshold value, judge that animal is in starvation, meet feeding requirement; When the time of feeding exceedes the time of setting, judge not meet feeding requirement.
Further, this stimulating system also comprises: animal position indicator; Described animal position indicator is fixed on described test casing top by electric expansion bar, and when needs do electro physiology experiment, described animal position indicator, under the control of extraneous control instruction, drops on described glass plate by described electric expansion bar.
Further, described animal positioner comprises: for the first fixture of fixing animal ear, for fixing animal face the second fixture, with rule and the support of the shape that takes the shape of the letter U, electric expansion bar, the first slide unit, the second slide unit; Described first fixture is connected respectively by the two ends with rule of described first slide unit with the support of the described shape that takes the shape of the letter U; Described first fixture is provided with adjustment bolt, for adjusting described first fixture Distance geometry described first slide unit of adjustment distance in the horizontal direction in vertical direction respectively; Described second fixture comprises Support bracket, the clip be made up of arc shaped hook and lateral frame; Described Support bracket is connected with described lateral frame; Described arc shaped hook is separately fixed at the two ends of described lateral frame; Described lateral frame is through described second slide unit; Described second slide unit is provided with adjustment bolt, for adjusting described Support bracket Distance geometry described second slide unit of adjustment distance in the vertical direction in the horizontal direction respectively; Described electric expansion bar one end is fixed on described test casing top, and the other end is fixedly connected with described second slide unit, and when doing electro physiology experiment, the control command according to the external world is stretched, and is placed on described glass plate by described brain positioner; After electro physiology experiment, the control command according to the external world is stretched, and described brain positioner is retracted to described test box top.
The present invention also provides a kind of animal stimulating method of described animal stimulating apparatus, comprising:
By the stimulating apparatus being arranged on test casing, animal is stimulated; Described stimulating apparatus comprises light stimulus module, Sound stimulat module and electrical stimulation module; Described light stimulus module comprises stimulating light source and control circuit; Shuttle back and forth on fence or the top being arranged on described test casing or sidewall described in described stimulating light source is arranged on, described control circuit is electrically connected with described Analysis Control Unit, and the control command for receiving described Analysis Control Unit controls the opening and closing of described stimulating light source intensity of illumination, light application time and/or light source; Described Sound stimulat module installation, in described test casing, comprises audio-frequency power amplifier and sound source; Shuttle back and forth on fence or the top being arranged on described test casing or sidewall described in described sound source is arranged on, and be electrically connected with described Analysis Control Unit and described audio-frequency power amplifier, the control command for receiving described Analysis Control Unit controls the frequency of described sound, amplitude, duration and/or decibel; Described electrical stimulation module comprises electric stimulating instrument and exposed copper cash; It is external that described electric stimulating instrument is arranged on test box, and exposed copper cash is laid on one side of something of glass plate, and the output line of described electric stimulating instrument is electrically connected with exposed copper cash by the hole being located at test casing;
Gathered by the characteristic information of harvester to animal being arranged on test casing; Described harvester comprises camera head, speed acquisition device, temperature collecting device and voice collection device; Described camera head is separately positioned in described test casing top and described accommodation space, wherein, the camera head being arranged on described test casing top is for gathering the behavioural characteristic of animal and aspectual character thereof, the video data of collection for gathering the footprint of animal and aspectual character thereof, and is sent to described Analysis Control Unit by camera head in real time that be arranged on described test casing accommodation space inner bottom part; Described camera head is connected with described Analysis Control Unit by video card; Described voice collection device is connected with described Analysis Control Unit by interface circuit card, for gathering noise audio frequency and/or animal sounds, and the voice data of collection is sent to described Analysis Control Unit in real time; Described electrical stimulation module is connected with described Analysis Control Unit by interface circuit card; Described speed acquisition device is connected with described Analysis Control Unit by interface circuit card, for gathering the speed of animal, and the speed data of collection is sent to described Analysis Control Unit in real time; Described temperature collecting device for gathering the temperature in described test casing, and is provided with the Analysis Control Unit of analysis and Control software described in the temperature of collection being sent in real time;
Analyzed by the experimental data of described Analysis Control Unit to the described video acquisition device received, audio collecting device and described temperature collecting device collection, send light stimulus instruction to described light stimulus module, send Sound stimulat instruction to described Sound stimulat module, send electro photoluminescence instruction to described electrical stimulation module, send to described food supply device and whether carry out food supply instruction, send temperature control instruction to described heater or described refrigerating plant and send animal dead reporting to the police; Described food supply device is arranged on described accommodation space and is positioned at the below of the opening of described glass plate, and the control instruction for sending according to described Analysis Control Unit controls to carry out feeding to animal; Wherein, described food supply device comprises pallet, expansion link, motor, telescopic jig arm and microprocessor; Described microprocessor is connected with described Analysis Control Unit by interface circuit card, and the control instruction for sending according to described Analysis Control Unit controls expansion link described in motor driving and stretches, thus is convenient to feeding animal; Described pallet is square, and centre is provided with dividing plate, for food and moisture being separated; Described jig arm is connected with described microprocessor, after animal has reached food meal requirement, under described microprocessor-based control, by animal folder from described pallet; The described control instruction for sending according to described Analysis Control Unit controls to carry out feeding to animal and comprises: analyzed the animal character information that described harvester gathers by described Analysis Control Unit, analyze and whether meet feeding requirement, when animal meets feeding requirement, control instruction is sent to described microprocessor, stretched by expansion link described in described Microprocessor S3C44B0X motor driving, control described opening by opening opening and closing device to open simultaneously, described pallet is delivered to described opening part, and parallel with the glass plate of opening part; When animal does not meet feeding requirement, control instruction is sent to described microprocessor, stretched by expansion link described in described Microprocessor S3C44B0X motor driving, described pallet is reduced to below described opening, control described opening by opening opening and closing device simultaneously and close.
Further, described Analysis Control Unit comprises: be provided with the database server of the database and control software design for storing data and be provided with the mobile terminal of analysis software; Described database server, receive the data message of described harvester collection and receive the control instruction of described mobile terminal by the transmission of wireless or internet, and control described harvester and stimulating apparatus according to described control instruction, and the data message of collection is sent to described mobile terminal; Described mobile terminal, the data message that database server described in Real-time Obtaining obtains, in real time by wireless or internet to described database server sending controling instruction, and the data that described database server gathers to be analyzed the behavioural information of animal or electrophysiology information.
Further, described in real time by wireless or internet to described database server sending controling instruction, and the data that described database server is gathered to the behavioural information of animal or electrophysiology information carry out analysis comprise in real time by wireless or internet to described database server sending controling instruction; The data message of the animal obtained from described database server under different stimulated is analyzed; The animal footprint information that data message analyze of the described animal to obtaining from described database server under different stimulated comprises described database server obtains is analyzed, and analyzes the animal footprint under different stimulated, and sorts out; Described animal footprint information comprises touchdown time, hang time, step-length, pressure, left and right pin spacing, front and back footprint note spacing and step sequence; And/or the animal that described database server obtains is analyzed under different stimulations, analyze under different stimulated, animal is to the reaction mean value of the duration of the reaction of response latency of the reaction amplitude peak stimulated, stimulation, stimulation, the speed of stimulation, the sound frequency of stimulation and stimulation; And/or whether analyzing animal there is dead symptom; And/or according to the temperature data gathered, judge whether the temperature of testing in casing meets experiment needs.
Further, describedly by described Analysis Control Unit, the animal character information that described harvester gathers to be analyzed, analyzes and whether meet feeding and requires to include:
The sign information of the animal of camera head collection is analyzed; The sign information of described animal comprises the size in animal belly region;
When the size in described animal belly region is less than threshold value, judges that animal is in starvation, meet feeding requirement; When the size in described animal belly region is greater than threshold value, judges that animal is in fed to appetite state, do not meet feeding requirement.
Further, describedly by described Analysis Control Unit, the animal character information that described harvester gathers to be analyzed, analyze and whether meet feeding and requires to include: the speed of the animal of speed acquisition device collection and cry are analyzed; When the speed of described animal and/or cry exceed threshold value, judge that animal is in starvation, meet feeding requirement; When the time of feeding exceedes the time of setting, judge not meet feeding requirement.
Animal stimulating system provided by the invention, make to use the experimenter of mobile terminal all long-rangely can carry out remote control, transfer experimental data in real time, send experiment control instruction, because mobile terminal has portability, experimenter the is broken away from restriction of physical space, greatly facilitates the operation of experimenter, saves the time of experimenter.Due to be all by mobile terminal by long-range sending controling instruction to database server, then control each device by database server and carry out electric automation and operate, human factor can be reduced to zooperal interference, improve the accuracy of experimental result.
Accompanying drawing explanation
The structured flowchart of the animal stimulating system that Fig. 1 provides for the embodiment of the present invention;
Casing and in-built structural representation thereof is tested in the animal stimulating system that Fig. 2 provides for the embodiment of the present invention;
Copper cash distribution schematic diagram in the test casing that Fig. 3 provides for the embodiment of the present invention;
The structured flowchart of mobile terminal in the animal stimulating system that Fig. 4 provides for the embodiment of the present invention;
The structural representation of animal positioner in the animal stimulating system that Fig. 5 provides for the embodiment of the present invention;
The schematic flow sheet of the animal stimulating method that Fig. 6 provides for the embodiment of the present invention.
Embodiment
See Fig. 1, a kind of animal stimulating system that the embodiment of the present invention provides, it Analysis Control Unit comprising at least one test casing 130 and be provided with analysis and Control software.Analysis Control Unit comprises the database server 120 of database and the control software design be provided with for storing data and is provided with the mobile terminal 110 of analysis software.Database server 120 receives data message that harvester gathers and mobile terminal receive 110 control instruction by the transmission of wireless or internet, and control harvester and stimulating apparatus according to control instruction, and the data message of collection is sent to mobile terminal 110; The data message that mobile terminal 110 Real-time Obtaining database server 120 obtains, in real time by wireless or internet to database server 120 sending controling instruction, and the data that database server 120 gathers to be analyzed the behavioural information of animal or electrophysiology information.
Stimulating apparatus, harvester, casing light source, heater and refrigerating plant is provided with in test casing 130.See Fig. 2, test casing 130 is provided with thick PVC base and stainless steel faceplate is formed.Glass plate 1 is provided with in test casing.It is interior and parallel with the base of test casing that glass plate 1 is arranged on test casing, and form accommodation space between the base of glass plate 1 and test casing; One jiao of glass plate 1 is provided with opening 7; Opening 7 place is provided with opening opening and closing device, and for when carrying out feeding animal, Electronic control opens opening 7, and when feeding animal is complete, Electronic control closes opening 7.Test casing is divided into two intervals by the fence 4 that movably shuttles back and forth.The fence 4 that shuttles back and forth is arranged in the middle part of glass plate 1, and is provided with and carries out for animal the door 5 that shuttles back and forth that shuttles back and forth.Test box external body is provided with sound proof box (not shown), and test casing top or sidewall are provided with the fan (not shown) providing ventilation function.Stimulating apparatus comprises light stimulus module, Sound stimulat module and electrical stimulation module.Light stimulus module comprises stimulates stimulating light source and control circuit; Stimulating light source is arranged on the fence 4 or be arranged on the test top of casing or sidewall of shuttling back and forth.Stimulating light source can be light emitting diode matrix.Control circuit is electrically connected with the database server in Analysis Control Unit, and the control command for receiving and analyzing control device controls the opening and closing of stimulating light source intensity of illumination, light application time and/or light source.Specifically: this control command is mobile terminal is the control instruction sent to database server 120 by wireless or internet, then control according to control instruction the opening and closing that control circuit controls stimulating light source intensity of illumination, light application time and/or light source by database server 120.Control circuit is prior art, and concrete structure repeats no more.Sound stimulat module installation, in test casing, comprises audio-frequency power amplifier and sound source; Sound source is arranged on the fence 4 or be arranged on the test top of casing or sidewall of shuttling back and forth, and be electrically connected with the database server in Analysis Control Unit and audio-frequency power amplifier, the control command for receiving and analyzing control device controls the frequency of sound, amplitude, duration and/or decibel.Such as, pure tone: frequency: 10-35000Hz, amplitude: 20-100dB, increases/reduces time 1ms-1s, the duration: 1s-1min.White noise: frequency=0, other are the same with pure tone.Specifically: this control command is mobile terminal is the control instruction sent to database server 120 by wireless or internet, then the frequency of sound, amplitude, duration and/or decibel is controlled by database server 120 according to control instruction.See Fig. 3, electrical stimulation module comprises electric stimulating instrument 150 and exposed copper cash 140; It is external that electric stimulating instrument 150 is arranged on test box, and exposed copper cash 140 is laid on one side of something of glass plate 1.The output line of electric stimulating instrument 150 is electrically connected with exposed copper cash 140 by the hole being located at test casing.Electric stimulating instrument is connected with Analysis Control Unit.Electric stimulating instrument 150 is specifically designed to the stimulation test of low pressure, eliminates the harm to animal.Electricity gate voltage adjusting range: 30V ~ 120V (square wave peak value), adjustment step-length 1V; Electricity grid current limliting adjusting range: 0.06mA ~ 4.00mA, adjustment step-length 0.02mA.Specifically: this control command is mobile terminal is the control instruction sent to database server 120 by wireless or internet, then control electricity thorn instrument by database server 120 according to control instruction to discharge.Harvester comprises camera head 2, camera head 3, speed acquisition device, temperature collecting device and voice collection device.Camera head 2 is arranged on test test casing top, camera head 3 is located at test casing accommodation space inner bottom part and is positioned at accommodation space, wherein, be arranged on the camera head 2 at test casing top for gathering the behavioural characteristic of animal and aspectual character thereof, the video data of collection for gathering the footprint of animal and aspectual character thereof, and is sent to Analysis Control Unit by camera head 3 in real time that be arranged on test casing accommodation space inner bottom part.Camera head 2 is connected with the database server in Analysis Control Unit by video card with camera head 3; Voice collection device is connected with the database server in Analysis Control Unit by interface circuit card, for gathering noise audio frequency and/or animal sounds, and the voice data of collection is sent to Analysis Control Unit in real time.Electrical stimulation module is connected with Analysis Control Unit by interface circuit card; Speed acquisition device is connected with the database server in Analysis Control Unit by interface circuit card, for gathering the speed of animal, and the speed data of collection is sent to the database server in Analysis Control Unit in real time; Temperature collecting device is used for the temperature in collecting test casing, and the temperature of collection is sent in real time the database server in Analysis Control Unit; Analysis Control Unit is used for the analysis according to the experimental data gathered the video acquisition device received, audio collecting device and temperature collecting device, send light stimulus instruction to light stimulus module, send Sound stimulat instruction to Sound stimulat module, send electro photoluminescence instruction to electrical stimulation module, send to food supply device and whether carry out food supply instruction and send temperature control instruction to heater or refrigerating plant, and send animal dead alarm.Specifically: the database server in Analysis Control Unit receives video acquisition device, the experimental data that audio collecting device and temperature collecting device gather, mobile terminal transfers the experimental data of collection from the database server Analysis Control Unit, then the analysis software arranged according to mobile terminal self is analyzed experimental data, then light stimulus instruction is sent to light stimulus module, Sound stimulat instruction is sent to Sound stimulat module, electro photoluminescence instruction is sent to electrical stimulation module, send to food supply device and whether carry out food supply instruction and send temperature control instruction to heater or refrigerating plant, and send animal dead alarm.
Food supply device is arranged on accommodation space and is positioned at the below of the opening of glass plate 1, and the control instruction for sending according to Analysis Control Unit controls to carry out feeding to animal; Wherein, see Fig. 2, food supply device comprises pallet 8, expansion link 12, motor 10, telescopic jig arm 11 and microprocessor (not shown); Microprocessor is connected with the database server in Analysis Control Unit by interface circuit card, and the control instruction for sending according to Analysis Control Unit controls motor 10 and drives expansion link 12 to stretch, thus is convenient to feeding animal.Pallet 8 is square, and centre is provided with dividing plate 9, for food and moisture being separated; Jig arm 11 is connected with microprocessor, after animal has reached food meal requirement, under control of the microprocessor, by animal folder from pallet 8.Wherein, control instruction for sending according to Analysis Control Unit controls to carry out feeding to animal and comprises: the experimental data obtained by mobile terminal called data storehouse server, i.e. animal character information, and animal character information is analyzed, analyze and whether meet feeding requirement (as how to judge, to describe in detail below), when animal meets feeding requirement, mobile terminal sends feeding instruction to database server, and then send control instruction by database server to microprocessor, expansion link 12 is driven to stretch by Microprocessor S3C44B0X motor 10, control opening 7 by opening opening and closing device to open simultaneously, by pallet 8 to described opening part, and it is parallel with the glass plate at opening 7 place, when animal does not meet feeding requirement, send control instruction to microprocessor, drive expansion link 12 to stretch by Microprocessor S3C44B0X motor 10, be reduced to by pallet 8 below opening 7, the opening 7 of opening opening and closing device control is simultaneously closed.
Opening opening and closing device comprises microprocessor, square glass, motor 15, rope 14 and mechanical arm 13.Mechanical arm 13 is fixedly connected with square glass.Square glass and opening 7 slitless connection.Motor 15 is connected with microprocessor and rope 14 respectively, for receiving microprocessor-based control order, controlling rope 14 and stretching, fallen or put down square glass, thus having realized the opening and closing of opening; Motor 15 is fixed on the sidewall of test casing; Microprocessor is connected with Analysis Control Unit, for the control instruction that receiving and analyzing control device sends, controls rope 14 and stretches.Four limits of square glass are ramped shaped, and four limits of opening 7 are ramped shaped, thus realize the slitless connection of square glass and opening 7, facilitate square glass can realize the disengaging with opening 7 smoothly simultaneously.By the long-range Automated condtrol to food supply device and opening opening and closing device, artificial feed effectively can be reduced on zooperal impact.
See Fig. 4, mobile terminal 110 comprises data acquisition module 200, analysis module 210, control instruction sending module 220 and alarm module 230.The data message of animal under different stimulated that data acquisition module 200 obtains from database server 120.Analysis module 210 is analyzed the data message of animal under different stimulated obtained from database server 120.Wherein, the animal footprint information that data message analyze of animal under different stimulated obtained from database server 120 comprises data storehouse server 120 obtains is analyzed, analyze the animal footprint under different stimulated, and sort out; Animal footprint information comprises touchdown time, hang time, step-length, pressure, left and right pin spacing, front and back footprint note spacing and step sequence; And/or the animal that database server obtains is analyzed under different stimulations, analyze under different stimulated, animal is to the reaction mean value of the duration of the reaction of response latency of the reaction amplitude peak stimulated, stimulation, stimulation, the speed of stimulation, the sound frequency of stimulation and stimulation; And/or judge whether animal meets feeding requirement according to the characteristic information of animal; And/or whether analyzing animal there is dead symptom; And/or according to the temperature data gathered, judge whether the temperature of testing in casing meets experiment needs; Alarm module 230, for when dead symptom appears in animal, sends warning message to user, to change laboratory animal in time.Control instruction sending module 220 is for database server 120 sending controling instruction.
Wherein, analyze and whether meet feeding and require to include: the sign information of the animal of camera head collection is analyzed; The sign information of described animal comprises the size in animal belly region; When the size in animal belly region is less than threshold value, judges that animal is in starvation, meet feeding requirement; When the size in animal belly region is greater than threshold value, judges that animal is in fed to appetite state, do not meet feeding requirement.In concrete example, can on pretreatment, setting respective threshold, that is, collection animal is in size a when having enough state, and collection animal is in size b during starvation, and a>b, can get a, b median between the two as threshold value.For mouse, can measure the mouse that will test in advance, measure the size of the belly being in mouse under starvation, measure the size of the belly of the mouse be under fed to appetite state, then take out a median as threshold value.When testing, the video information being arranged on the collection of accommodation space photographic device inside can be relied on to analyze the size of mouse belly to the size in mouse belly region.In order to improve the accuracy of analysis further, analysis can be combined to the video information of the camera head collection being arranged on accommodation space photographic device inside and being arranged on test casing top, namely, be arranged on the bottom section that accommodation space photographic device inside mainly gathers mouse belly, and the camera head at casing top gathers the two side areas of mouse belly, go out the change of mouse belly size from the mutation analysis of bottom section and two side areas, and then judge whether mouse is in starvation.In another embodiment, also can analyze by the speed of the animal to the collection of speed acquisition device and cry; When the speed of described animal and/or cry exceed setting threshold value (, the speed of animal exceedes the threshold speed of setting, or cry exceedes the decibel of setting, or cry exceedes the time etc. of setting), judge that animal is in starvation, meet feeding requirement; When the time of feeding exceedes the time of setting, judge not meet feeding requirement.By with upper type, effectively can ensure the reasonable feed of animal, avoid occurring crapulent situation.
Because the situation of experimental situation is changeable, such as, summer compares higher temperatures, winter is colder, this has applicable temperature environment with regard to needs guarantee test casing, so by the temperature in temperature collecting device Real-time Collection test casing, and the database server temperature of collection is sent in real time in Analysis Control Unit, after mobile terminal obtains these temperature datas from database server, whether the temperature judging to test casing meets the temperature threshold of setting, when temperature is lower than temperature threshold, send heating instructions, control heater and heat; When temperature is higher than temperature threshold, send refrigeration instruction, control refrigerating plant and freeze, thus the temperature controlling test casing meets the temperature threshold of setting.
In addition, in order to due in experimental situation, animal dead phenomenon can be there is unavoidably, this just needs the very first time to notify experimenter, so that experimenter changes laboratory animal in time, ensure normally carrying out of experiment, so just need send animal dead alarm in time when animal dead.Wherein, send animal dead alarm to comprise: judge whether animal occurs Death actualty, such as, find that animal is motionless for a long time by video acquisition device, and after occurring that pupil sends out large or that health is stiff present situation, in time to using the user of mobile terminal to send dead warning message.
Above control mode, use the experimenter of mobile terminal all long-rangely can carry out remote control, transfer experimental data in real time, send experiment control instruction, because mobile terminal has portability, experimenter the is broken away from restriction of physical space, greatly facilitates the operation of experimenter, saves the time of experimenter.Such as, test in Beijing, go on business the U.S., also can the related data of Real-time Obtaining experimental field in the U.S., sends control instruction.Due to be all by mobile terminal by long-range sending controling instruction to database server, then control each device by database server to carry out electric automation and operate, such as, feeding, thus human factor can be reduced to zooperal interference, improve the accuracy of experimental result.
See Fig. 5, in order to do electro physiology experiment, animal stimulating system also comprises animal position indicator.Animal position indicator is fixed on test casing top by electric expansion bar 21, and when needs do electro physiology experiment, animal position indicator, under the control of extraneous control instruction, drops on glass plate by electric expansion bar 21.Animal positioner comprises the first fixture 16 for fixing animal ear, for fixing animal face the second fixture, with rule and the support 24 of the shape that takes the shape of the letter U, electric expansion bar 21, first slide unit 23 and the second slide unit 28.First fixture 16 is connected respectively by the two ends with rule of the first slide unit 23 with the support of the shape that takes the shape of the letter U.First fixture is provided with adjustment bolt 25 and adjustment bolt 18, adjusts the first slide unit 23 distance in the horizontal direction for adjusting the first fixture Distance geometry in vertical direction respectively.Second fixture comprises Support bracket 27, the clip 17 be made up of arc shaped hook and lateral frame 26.Support bracket 27 is connected with lateral frame 26.Arc shaped hook 17 is separately fixed at the two ends of lateral frame 26.Lateral frame 26 is through the second slide unit 28.Second slide unit 28 is provided with two adjustment bolts (wherein, one is adjustment bolt 29, another adjustment bolt is not shown), adjust the second slide unit 28 distance in the vertical direction for adjusting Support bracket 27 Distance geometry in the horizontal direction respectively.Electric expansion bar 21 one end is fixed on test casing top, and the other end is fixedly connected with the second slide unit 28, and when doing electro physiology experiment, the control command according to the external world is stretched, and is placed on glass plate by brain positioner; After electro physiology experiment, the control command according to the external world is stretched, and brain positioner is retracted to test box top.
See Fig. 6, based on the animal stimulating method of the stimulating apparatus of animal shown in Fig. 1-5, comprising:
Step 300: animal is stimulated by the stimulating apparatus being arranged on test casing; Described stimulating apparatus comprises light stimulus module, Sound stimulat module and electrical stimulation module; Described light stimulus module comprises stimulating light source and control circuit; Shuttle back and forth on fence or the top being arranged on described test casing or sidewall described in described stimulating light source is arranged on, described control circuit is electrically connected with described Analysis Control Unit, and the control command for receiving described Analysis Control Unit controls the opening and closing of described stimulating light source intensity of illumination, light application time and/or light source; Described Sound stimulat module installation, in described test casing, comprises audio-frequency power amplifier and sound source; Shuttle back and forth on fence or the top being arranged on described test casing or sidewall described in described sound source is arranged on, and be electrically connected with described Analysis Control Unit and described audio-frequency power amplifier, the control command for receiving described Analysis Control Unit controls the frequency of described sound, amplitude, duration and/or decibel; Described electrical stimulation module comprises electric stimulating instrument and exposed copper cash; It is external that described electric stimulating instrument is arranged on test box, and exposed copper cash is laid on one side of something of glass plate, and the output line of described electric stimulating instrument is electrically connected with exposed copper cash by the hole being located at test casing;
Step 400, by be arranged on test casing the characteristic information of harvester to animal gather; Described harvester comprises camera head, speed acquisition device, temperature collecting device and voice collection device; Described camera head is separately positioned in described test casing top and described accommodation space, wherein, the camera head being arranged on described test casing top is for gathering the behavioural characteristic of animal and aspectual character thereof, the video data of collection for gathering the footprint of animal and aspectual character thereof, and is sent to described Analysis Control Unit by camera head in real time that be arranged on described test casing accommodation space inner bottom part; Described camera head is connected with described Analysis Control Unit by video card; Described voice collection device is connected with described Analysis Control Unit by interface circuit card, for gathering noise audio frequency and/or animal sounds, and the voice data of collection is sent to described Analysis Control Unit in real time; Described electrical stimulation module is connected with described Analysis Control Unit by interface circuit card; Described speed acquisition device is connected with described Analysis Control Unit by interface circuit card, for gathering the speed of animal, and the speed data of collection is sent to described Analysis Control Unit in real time; Described temperature collecting device for gathering the temperature in described test casing, and is provided with the Analysis Control Unit of analysis and Control software described in the temperature of collection being sent in real time;
Step 500, to be analyzed by the experimental data of described Analysis Control Unit to the described video acquisition device received, audio collecting device and described temperature collecting device collection, send light stimulus instruction to described light stimulus module, send Sound stimulat instruction to described Sound stimulat module, send electro photoluminescence instruction to described electrical stimulation module, send to described food supply device and whether carry out food supply instruction, send temperature control instruction to described heater or described refrigerating plant and send animal dead reporting to the police; Described food supply device is arranged on described accommodation space and is positioned at the below of the opening of described glass plate, and the control instruction for sending according to described Analysis Control Unit controls to carry out feeding to animal; Wherein, described food supply device comprises pallet, expansion link, motor, telescopic jig arm and microprocessor; Described microprocessor is connected with described Analysis Control Unit by interface circuit card, and the control instruction for sending according to described Analysis Control Unit controls expansion link described in motor driving and stretches, thus is convenient to feeding animal; Described pallet is square, and centre is provided with dividing plate, for food and moisture being separated; Described jig arm is connected with described microprocessor, after animal has reached food meal requirement, under described microprocessor-based control, by animal folder from described pallet; The described control instruction for sending according to described Analysis Control Unit controls to carry out feeding to animal and comprises: analyzed the animal character information that described harvester gathers by described Analysis Control Unit, analyze and whether meet feeding requirement, when animal meets feeding requirement, control instruction is sent to described microprocessor, stretched by expansion link described in described Microprocessor S3C44B0X motor driving, control described opening by opening opening and closing device to open simultaneously, described pallet is delivered to described opening part, and parallel with the glass plate of opening part; When animal does not meet feeding requirement, control instruction is sent to described microprocessor, stretched by expansion link described in described Microprocessor S3C44B0X motor driving, described pallet is reduced to below described opening, control described opening by opening opening and closing device simultaneously and close.
In above-mentioned steps, Analysis Control Unit comprises the database server of the database and control software design be provided with for storing data and is provided with the mobile terminal of analysis software; Described database server, receive the data message of described harvester collection and receive the control instruction of described mobile terminal by the transmission of wireless or internet, and control described harvester and stimulating apparatus according to described control instruction, and the data message of collection is sent to described mobile terminal; Described mobile terminal, the data message that database server described in Real-time Obtaining obtains, in real time by wireless or internet to described database server sending controling instruction, and the data that described database server gathers to be analyzed the behavioural information of animal or electrophysiology information.
Wherein, in real time by wireless or internet to described database server sending controling instruction, and to the behavioural information of animal or electrophysiology information, analysis carried out to the data that described database server gathers comprise: in real time by wireless or internet to described database server sending controling instruction; The data message of the animal obtained from described database server under different stimulated is analyzed; The animal footprint information that data message analyze of the described animal to obtaining from described database server under different stimulated comprises described database server obtains is analyzed, and analyzes the animal footprint under different stimulated, and sorts out; Described animal footprint information comprises touchdown time, hang time, step-length, pressure, left and right pin spacing, front and back footprint note spacing and step sequence; And/or the animal that described database server obtains is analyzed under different stimulations, analyze under different stimulated, animal is to the reaction mean value of the duration of the reaction of response latency of the reaction amplitude peak stimulated, stimulation, stimulation, the speed of stimulation, the sound frequency of stimulation and stimulation; And/or whether analyzing animal there is dead symptom; And/or according to the temperature data gathered, judge whether the temperature of testing in casing meets experiment needs.
Wherein, in one embodiment, analyzed the animal character information that described harvester gathers by described Analysis Control Unit, whether analysis meets feeding requires to include: analyze the sign information of the animal of camera head collection; The sign information of described animal comprises the size in animal belly region; When the size in described animal belly region is less than threshold value, judges that animal is in starvation, meet feeding requirement; When the size in described animal belly region is greater than threshold value, judges that animal is in fed to appetite state, do not meet feeding requirement.
In another embodiment, describedly by described Analysis Control Unit, the animal character information that described harvester gathers to be analyzed, analyze and whether meet feeding and require to include the speed of the animal of speed acquisition device collection and cry are analyzed; When the speed of described animal and/or cry exceed threshold value, judge that animal is in starvation, meet feeding requirement; When the time of feeding exceedes the time of setting, judge not meet feeding requirement.
The animal stimulating method that the embodiment of the present invention provides, make to use the experimenter of mobile terminal all long-rangely can carry out remote control, transfer experimental data in real time, send experiment control instruction, because mobile terminal has portability, experimenter the is broken away from restriction of physical space, greatly facilitates the operation of experimenter, saves the time of experimenter.Such as, test in Beijing, go on business the U.S., also can the related data of Real-time Obtaining experimental field in the U.S., sends control instruction.Due to be all by mobile terminal by long-range sending controling instruction to database server, then control each device by database server to carry out electric automation and operate, such as, feeding, thus human factor can be reduced to zooperal interference, improve the accuracy of experimental result.
It should be noted last that, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although with reference to example to invention has been detailed description, those of ordinary skill in the art is to be understood that, can modify to technical scheme of the present invention or equivalent replacement, and not departing from the spirit and scope of technical solution of the present invention, it all should be encompassed in the middle of right of the present invention.
A10, animal stimulating method based on animal stimulating apparatus, comprising:
By the stimulating apparatus being arranged on test casing, animal is stimulated; Described stimulating apparatus comprises light stimulus module, Sound stimulat module and electrical stimulation module; Described light stimulus module comprises stimulating light source and control circuit; Shuttle back and forth on fence or the top being arranged on described test casing or sidewall described in described stimulating light source is arranged on, described control circuit is electrically connected with described Analysis Control Unit, and the control command for receiving described Analysis Control Unit controls the opening and closing of described stimulating light source intensity of illumination, light application time and/or light source; Described Sound stimulat module installation, in described test casing, comprises audio-frequency power amplifier and sound source; Shuttle back and forth on fence or the top being arranged on described test casing or sidewall described in described sound source is arranged on, and be electrically connected with described Analysis Control Unit and described audio-frequency power amplifier, the control command for receiving described Analysis Control Unit controls the frequency of described sound, amplitude, duration and/or decibel; Described electrical stimulation module comprises electric stimulating instrument and exposed copper cash; It is external that described electric stimulating instrument is arranged on test box, and exposed copper cash is laid on one side of something of glass plate, and the output line of described electric stimulating instrument is electrically connected with exposed copper cash by the hole being located at test casing;
Gathered by the characteristic information of harvester to animal being arranged on test casing; Described harvester comprises camera head, speed acquisition device, temperature collecting device and voice collection device; Described camera head is separately positioned in described test casing top and described accommodation space, wherein, the camera head being arranged on described test casing top is for gathering the behavioural characteristic of animal and aspectual character thereof, the video data of collection for gathering the footprint of animal and aspectual character thereof, and is sent to described Analysis Control Unit by camera head in real time that be arranged on described test casing accommodation space inner bottom part; Described camera head is connected with described Analysis Control Unit by video card; Described voice collection device is connected with described Analysis Control Unit by interface circuit card, for gathering noise audio frequency and/or animal sounds, and the voice data of collection is sent to described Analysis Control Unit in real time; Described electrical stimulation module is connected with described Analysis Control Unit by interface circuit card; Described speed acquisition device is connected with described Analysis Control Unit by interface circuit card, for gathering the speed of animal, and the speed data of collection is sent to described Analysis Control Unit in real time; Described temperature collecting device for gathering the temperature in described test casing, and is provided with the Analysis Control Unit of analysis and Control software described in the temperature of collection being sent in real time;
Analyzed by the experimental data of described Analysis Control Unit to the described video acquisition device received, audio collecting device and described temperature collecting device collection, send light stimulus instruction to described light stimulus module, send Sound stimulat instruction to described Sound stimulat module, send electro photoluminescence instruction to described electrical stimulation module, send to described food supply device and whether carry out food supply instruction, send temperature control instruction to described heater or described refrigerating plant and send animal dead reporting to the police; Described food supply device is arranged on described accommodation space and is positioned at the below of the opening of described glass plate, and the control instruction for sending according to described Analysis Control Unit controls to carry out feeding to animal; Wherein, described food supply device comprises pallet, expansion link, motor, telescopic jig arm and microprocessor; Described microprocessor is connected with described Analysis Control Unit by interface circuit card, and the control instruction for sending according to described Analysis Control Unit controls expansion link described in motor driving and stretches, thus is convenient to feeding animal; Described pallet is square, and centre is provided with dividing plate, for food and moisture being separated; Described jig arm is connected with described microprocessor, after animal has reached food meal requirement, under described microprocessor-based control, by animal folder from described pallet; The described control instruction for sending according to described Analysis Control Unit controls to carry out feeding to animal and comprises: analyzed the animal character information that described harvester gathers by described Analysis Control Unit, analyze and whether meet feeding requirement, when animal meets feeding requirement, control instruction is sent to described microprocessor, stretched by expansion link described in described Microprocessor S3C44B0X motor driving, control described opening by opening opening and closing device to open simultaneously, described pallet is delivered to described opening part, and parallel with the glass plate of opening part; When animal does not meet feeding requirement, control instruction is sent to described microprocessor, stretched by expansion link described in described Microprocessor S3C44B0X motor driving, described pallet is reduced to below described opening, control described opening by opening opening and closing device simultaneously and close.
A11, method according to A10, described Analysis Control Unit comprises:
Be provided with the database server of the database and control software design for storing data and be provided with the mobile terminal of analysis software;
Described database server, receive the data message of described harvester collection and receive the control instruction of described mobile terminal by the transmission of wireless or internet, and control described harvester and stimulating apparatus according to described control instruction, and the data message of collection is sent to described mobile terminal;
Described mobile terminal, the data message that database server described in Real-time Obtaining obtains, in real time by wireless or internet to described database server sending controling instruction, and the data that described database server gathers to be analyzed the behavioural information of animal or electrophysiology information.
A12, method according to A11, described in real time by wireless or internet to described database server sending controling instruction, and to the behavioural information of animal or electrophysiology information, analysis carried out to the data that described database server gathers comprise:
In real time by wireless or internet to described database server sending controling instruction; The data message of the animal obtained from described database server under different stimulated is analyzed; The animal footprint information that data message analyze of the described animal to obtaining from described database server under different stimulated comprises described database server obtains is analyzed, and analyzes the animal footprint under different stimulated, and sorts out; Described animal footprint information comprises touchdown time, hang time, step-length, pressure, left and right pin spacing, front and back footprint note spacing and step sequence; And/or the animal that described database server obtains is analyzed under different stimulations, analyze under different stimulated, animal is to the reaction mean value of the duration of the reaction of response latency of the reaction amplitude peak stimulated, stimulation, stimulation, the speed of stimulation, the sound frequency of stimulation and stimulation; And/or whether analyzing animal there is dead symptom; And/or according to the temperature data gathered, judge whether the temperature of testing in casing meets experiment needs.
A13, method according to A11, describedly analyzed the animal character information that described harvester gathers by described Analysis Control Unit, analyzes whether to meet feeding and requires to include:
The sign information of the animal of camera head collection is analyzed; The sign information of described animal comprises the size in animal belly region;
When the size in described animal belly region is less than threshold value, judges that animal is in starvation, meet feeding requirement; When the size in described animal belly region is greater than threshold value, judges that animal is in fed to appetite state, do not meet feeding requirement.
A14, method according to A11, describedly analyzed the animal character information that described harvester gathers by described Analysis Control Unit, analyzes whether to meet feeding and requires to include:
The speed of the animal of speed acquisition device collection and cry are analyzed;
When the speed of described animal and/or cry exceed threshold value, judge that animal is in starvation, meet feeding requirement;
When the time of feeding exceedes the time of setting, judge not meet feeding requirement.

Claims (9)

1. an animal stimulating system, is characterized in that, comprising:
At least one test casing, stimulating apparatus, the casing light source being arranged on described test casing top, harvester, the Analysis Control Unit being provided with analysis and Control software, heater and refrigerating plant;
Described test casing is made up of thick PVC base and stainless steel faceplate, glass plate is provided with in described test casing, it is interior and parallel with the base of described test casing that described glass plate is arranged on described test casing, and form accommodation space between the base of described glass plate and described test casing; One jiao of described glass plate is provided with opening; Described opening part is provided with opening opening and closing device, for when carrying out feeding animal, opens described opening, when feeding animal is complete, closes described opening; Described test casing is divided into two intervals by the fence that movably shuttles back and forth; The described fence that shuttles back and forth is arranged in the middle part of described glass plate, and is provided with and carries out for animal the door that shuttles back and forth that shuttles back and forth; Described test box external body is provided with sound proof box, and described test casing top or sidewall are provided with the fan providing ventilation function; Described stimulating apparatus comprises light stimulus module, Sound stimulat module and electrical stimulation module; Described light stimulus module comprises stimulating light source and control circuit; Shuttle back and forth on fence or the top being arranged on described test casing or sidewall described in described stimulating light source is arranged on, described control circuit is electrically connected with described Analysis Control Unit, and the control command for receiving described Analysis Control Unit controls the opening and closing of described stimulating light source intensity of illumination, light application time and/or light source; Described Sound stimulat module installation, in described test casing, comprises audio-frequency power amplifier and sound source; Shuttle back and forth on fence or the top being arranged on described test casing or sidewall described in described sound source is arranged on, and be electrically connected with described Analysis Control Unit and described audio-frequency power amplifier, control the frequency of sound, amplitude, duration and/or decibel for the control command receiving described Analysis Control Unit; Described electrical stimulation module comprises electric stimulating instrument and exposed copper cash; It is external that described electric stimulating instrument is arranged on test box, and exposed copper cash is laid on the side of glass plate, and the output line of described electric stimulating instrument is electrically connected with exposed copper cash by the hole being located at test casing; Described harvester comprises camera head, speed acquisition device, temperature collecting device and voice collection device; Described camera head is separately positioned in described test casing top and described accommodation space, wherein, the camera head being arranged on described test casing top is for gathering the behavioural characteristic of animal and aspectual character thereof, the video data of collection for gathering the footprint of animal and aspectual character thereof, and is sent to described Analysis Control Unit by camera head in real time that be arranged on described test casing accommodation space inner bottom part; Described camera head is connected with described Analysis Control Unit by video card; Described voice collection device is connected with described Analysis Control Unit by interface circuit card, for gathering noise audio frequency and/or animal sounds, and the voice data of collection is sent to described Analysis Control Unit in real time; Described electrical stimulation module is connected with described Analysis Control Unit by interface circuit card; Described speed acquisition device is connected with described Analysis Control Unit by interface circuit card, for gathering the speed of animal, and the speed data of collection is sent to described Analysis Control Unit in real time; Described temperature collecting device for gathering the temperature in described test casing, and is provided with the Analysis Control Unit of analysis and Control software described in the temperature of collection being sent in real time;
Described Analysis Control Unit is used for the analysis according to the experimental data gathered the described camera head received, voice collection device and described temperature collecting device, send light stimulus instruction to described light stimulus module, send Sound stimulat instruction to described Sound stimulat module, send electro photoluminescence instruction to described electrical stimulation module, send to food supply device and whether carry out food supply instruction, send temperature control instruction to described heater or described refrigerating plant, and send animal dead alarm;
Described food supply device is arranged on described accommodation space and is positioned at the below of the opening of described glass plate, and the control instruction for sending according to described Analysis Control Unit controls to carry out feeding to animal; Wherein, described food supply device comprises pallet, expansion link, motor, telescopic jig arm and microprocessor; Described microprocessor is connected with described Analysis Control Unit by interface circuit card, and the control instruction for sending according to described Analysis Control Unit controls expansion link described in motor driving and stretches, thus is convenient to feeding animal; Described pallet is square, and centre is provided with dividing plate, for food and moisture being separated; Described jig arm is connected with described microprocessor, after animal has reached food meal requirement, under described microprocessor-based control, by animal folder from described pallet;
The described control instruction for sending according to described Analysis Control Unit controls to carry out feeding to animal and comprises: analyzed the animal character information that described harvester gathers by described Analysis Control Unit, analyze and whether meet feeding requirement, when animal meets feeding requirement, control instruction is sent to described microprocessor, stretched by expansion link described in described Microprocessor S3C44B0X motor driving, control described opening by opening opening and closing device to open simultaneously, described pallet is delivered to described opening part, and parallel with the glass plate of opening part; When animal does not meet feeding requirement, control instruction is sent to described microprocessor, stretched by expansion link described in described Microprocessor S3C44B0X motor driving, described pallet is reduced to below described opening, control described opening by opening opening and closing device simultaneously and close.
2. animal stimulating system according to claim 1, is characterized in that, described Analysis Control Unit comprises:
Be provided with the database server of the database and control software design for storing data and be provided with the mobile terminal of analysis software;
Described database server, receive the data message of described harvester collection and receive the control instruction of described mobile terminal by the transmission of wireless or internet, and control described harvester, stimulating apparatus, heater, refrigerating plant and food supply device according to described control instruction, and the data message of collection is sent to described mobile terminal;
Described mobile terminal, the data message that database server described in Real-time Obtaining obtains, in real time by wireless or internet to described database server sending controling instruction, and the data that described database server gathers to be analyzed the behavioural information of animal or electrophysiology information.
3. animal stimulating system according to claim 2, is characterized in that, described mobile terminal comprises:
Data acquisition module, obtains the data message of animal under different stimulated from described database server;
Analysis module, analyzes the data message of the animal obtained from described database server under different stimulated; The animal footprint information that data message analyze of the described animal to obtaining from described database server under different stimulated comprises described database server obtains is analyzed, and analyzes the animal footprint under different stimulated, and sorts out; Described animal footprint information comprises touchdown time, hang time, step-length, pressure, left and right pin spacing, front and back footprint note spacing and step sequence; And/or the animal that described database server obtains is analyzed under different stimulations, analyze under different stimulated, animal is to the reaction mean value of the duration of the reaction of response latency of the reaction amplitude peak stimulated, stimulation, stimulation, the speed of stimulation, the sound frequency of stimulation and stimulation; And/or judge whether animal meets feeding requirement according to the characteristic information of animal; And/or whether analyzing animal there is dead symptom; And/or according to the temperature data gathered, judge whether the temperature of testing in casing meets experiment needs;
Control instruction sending module, the result analyzed according to described analysis module is to described database server sending controling instruction;
Alarm module, when dead symptom appears in animal, sends warning message to user, to change laboratory animal in time.
4. animal stimulating system according to claim 1, is characterized in that, described opening opening and closing device comprises:
Microprocessor, square glass, motor, rope and mechanical arm; Described mechanical arm is fixedly connected with described square glass; Described square glass and described opening slitless connection; Described motor is connected with described microprocessor and described rope respectively, for receiving described microprocessor-based control order, controlling described rope and stretching, sling or put down described square glass, thus realizing the opening and closing of described opening; Described motor is fixed on the sidewall of described test casing; Described microprocessor is connected with Analysis Control Unit.
5. animal stimulating system according to claim 4, it is characterized in that, four limits of described square glass are ramped shaped, and four limits of described opening are ramped shaped, thus realize the slitless connection of square glass and described opening, facilitate square glass can realize the disengaging with described opening smoothly simultaneously.
6. animal stimulating system according to claim 1, is characterized in that, is describedly analyzed the animal character information that described harvester gathers by described Analysis Control Unit, analyzes whether to meet feeding and requires to include:
The sign information of the animal of camera head collection is analyzed; The sign information of described animal comprises the size in animal belly region;
When the size in described animal belly region is less than threshold value, judges that animal is in starvation, meet feeding requirement; When the size in described animal belly region is greater than threshold value, judges that animal is in fed to appetite state, do not meet feeding requirement.
7. animal stimulating system according to claim 1, is characterized in that, is describedly analyzed the animal character information that described harvester gathers by described Analysis Control Unit, analyzes whether to meet feeding and requires to include:
The speed of the animal of speed acquisition device collection and cry are analyzed;
When the speed of described animal and/or cry exceed threshold value, judge that animal is in starvation, meet feeding requirement;
When the time of feeding exceedes the time of setting, judge not meet feeding requirement.
8. animal stimulating system according to claim 1, is characterized in that, also comprise: animal position indicator; Described animal position indicator is fixed on described test casing top by electric expansion bar, and when needs do electro physiology experiment, described animal position indicator, under the control of extraneous control instruction, drops on described glass plate by described electric expansion bar.
9. animal stimulating system according to claim 8, is characterized in that, described animal position indicator comprises:
For the first fixture of fixing animal ear, for fixing animal face the second fixture, with rule and the support of the shape that takes the shape of the letter U, electric expansion bar, the first slide unit, the second slide unit; Described first fixture is connected respectively by the two ends with rule of described first slide unit with the support of the described shape that takes the shape of the letter U; Described first fixture is provided with adjustment bolt, for adjusting described first fixture Distance geometry described first slide unit of adjustment distance in the horizontal direction in vertical direction respectively; Described second fixture comprises Support bracket, the clip be made up of arc shaped hook and lateral frame; Described Support bracket is connected with described lateral frame; Described arc shaped hook is separately fixed at the two ends of described lateral frame; Described lateral frame is through described second slide unit; Described second slide unit is provided with adjustment bolt, for adjusting described Support bracket Distance geometry described second slide unit of adjustment distance in the vertical direction in the horizontal direction respectively; Described electric expansion bar one end is fixed on described test casing top, and the other end is fixedly connected with described second slide unit, and when doing electro physiology experiment, the control command according to the external world is stretched, and is placed into by animal position indicator on described glass plate; After electro physiology experiment, the control command according to the external world is stretched, and described animal position indicator is retracted to described test casing top.
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