CN109977768B - Closed-loop feedback type animal behavior analysis system, method and device - Google Patents
Closed-loop feedback type animal behavior analysis system, method and device Download PDFInfo
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Abstract
The invention is suitable for the technical field of biomedicine, and provides a closed-loop feedback type animal behavior analysis system, a method and a device, wherein the system comprises the following components: the invention discloses an animal behavior analysis experimental device, a visual control platform and a third-party control platform, wherein the visual control platform analyzes first behavior characteristics of an animal according to first behavior data sent by the animal behavior analysis experimental device to obtain corresponding first behavior characteristic data, when current stimulation parameters containing the first behavior characteristic data meet external stimulation experimental requirements, the third-party control platform gives various stimulations to the animal according to external stimulation experimental instructions sent by the visual control platform, and the corresponding experimental parameters are changed until the requirements of an experimenter are met by feeding back the performance of the stimulated animal to the visual control platform, so that the system cost is reduced, accurate analysis of animal behaviors is realized, and research on the relation between corresponding brain areas or nerves of the animal and the motor behaviors is facilitated for researchers.
Description
Technical Field
The invention belongs to the technical field of biomedicine, and particularly relates to a closed-loop feedback type animal behavior analysis system, method and device.
Background
At present, animal behavior analysis systems (ANY-maze) which are commercial software are mainly used for animal analysis in the market, however, ANY-maze is high in charge and poor in flexibility, can only analyze animal behaviors in a specific mode and cannot meet specific requirements of users, the software can only be installed on one computer due to the fact that the password dog exists, the efficiency of researchers is reduced to a great extent, and the ANY-maze software is only suitable for small animals and is not suitable for large animals, and the application range is limited.
In the invention patent application document with the publication number of CN101214168B, an animal behavior monitoring and analyzing system is disclosed, which comprises an experimental device and a computer analysis software system, wherein the experimental device is combined with a video signal acquisition device based on infrared ray, optical-mechanical-electrical integration technology, Light-Emitting Diode (LED) array analysis technology and digital camera technology, the computer analysis software system has a function of real-time/off-line analysis of the acquired animal behavior signals, can provide a database, various data interfaces, and track and record the input real-time monitoring signals, and also has a function of processing a series of test data for each test item, so as to extract the behavior track and characteristics of the animal, and calculate a plurality of behavioral parameters based on the behavior track and characteristics, thereby facilitating the recording of the monitoring data, The stored and later data analysis ensures that the test data has strong repeatability and high reliability, thereby having strong operability and practicability. Because the video signal acquisition device based on the infrared ray, the optical-mechanical-electrical integration technology, the LED array analysis technology and the digital camera technology is combined in the experimental device of the animal behavior monitoring and analyzing system, the animal behavior monitoring and analyzing system is complex in construction and operation and high in cost, is only suitable for specific paradigms, such as a Morri water maze, a shuttle box and the like, is limited in application range, is not suitable for large animals and the like, and cannot display the motion characteristics of the animals in real time.
In the invention patent application document with the patent publication number of CN105684935A, an animal behavior analysis method and system are disclosed, in which the animal shape is detected successively at a first time and a second time, and corresponding first animal shape information and second animal shape information are obtained, the second animal shape information is compared with the first animal shape information to obtain shape change information, corresponding animal behavior information is obtained from an animal behavior database according to the shape change information, that is, a detection device is used to detect the animal shape information, and the shape information obtained by the detection device is intelligently analyzed to obtain the animal behavior information. However, the animal behavior analysis method mainly detects and compares the shape information of the animal at different moments, and only can analyze large state changes of the animal, such as standing, squatting and the like, and cannot accurately analyze the behavior of the animal, so that the method has the defects of low algorithm precision and small application range, cannot analyze and display the behavior variable of the animal in real time, does not have closed-loop feedback, and is not beneficial for experimenters to adjust in time according to own needs.
Meanwhile, software for detecting the contact times of the two animals and the total times and the total time of the animals reaching a specific area are not available in the market, the statistics of the contact times of the two animals and the times and the time of the animals reaching the specific area still remain in a manual counting stage, the time is wasted, and errors are easy to make. Among the various stimulation patterns used for animal behavior analysis, one specific example of the visual stimulation pattern is to analyze the rigor status (Freezing) and fast running (Flate) behavior of mice under visual stimulation, however, the current phase does not have software for counting the starting time and ending time of each of Freezing and Flate, nor the total time and number of Freezing and Flate during the whole process.
Therefore, a new animal behavior analysis method is needed to solve the above problems.
Disclosure of Invention
The invention aims to provide a closed-loop feedback type animal behavior analysis system, method and device, and aims to solve the problems that the prior art cannot provide an effective animal behavior analysis method, so that the experimental cost of animal behavior analysis is high, and the animal behavior analysis is relatively rough and unilateral.
In one aspect, the present invention provides a closed loop feedback animal behavior analysis system, the system comprising:
the animal behavior analysis experimental device is used for generating external stimulation acting on an animal body, acquiring a corresponding animal behavior video, and performing image processing on the animal behavior video through a preset image processing algorithm to acquire corresponding behavior data, wherein the animal behavior video is an online video corresponding to the animal under the current activity acquired in real time or a stored offline video;
the visual control platform is connected with the animal experiment device and used for analyzing the behavior characteristics of the animal according to the received behavior data sent by the animal behavior analysis experiment device to obtain behavior characteristic data corresponding to the behavior characteristics and determining whether the behavior characteristic data meets the preset external stimulation experiment requirement or not so as to generate a corresponding external stimulation experiment instruction; and
and the third-party control platform is connected with the animal behavior analysis experiment device and the visual control platform and is used for controlling the corresponding stimulation paradigm according to the received external stimulation experiment instruction sent by the visual control platform, so that the animal behavior analysis experiment device generates the external stimulation acting on the animal body.
In another aspect, the present invention provides an animal behavior analysis method of the closed-loop feedback type animal behavior analysis system, including the steps of:
when a request for animal behavior analysis of the animal is received, the animal behavior analysis experimental device obtains a corresponding animal behavior video, and performs image processing on the animal behavior video through a preset image processing algorithm to obtain corresponding first behavior data, wherein the animal behavior video is an online video corresponding to the animal in the current activity acquired in real time or a stored offline video;
when the animal behavior video is an online video, the visual control platform analyzes the first behavior feature of the animal under the current activity according to the received first behavior data sent by the animal behavior analysis experiment device to obtain first behavior feature data corresponding to the first behavior feature;
detecting current stimulation parameters, wherein the current stimulation parameters comprise the first behavior characteristic data, the current stimulation times and the current stimulation interval duration;
judging whether the detected current stimulation parameters meet preset external stimulation experiment requirements or not;
if so, the visual control platform generates a corresponding external stimulation experiment instruction according to the current stimulation parameter and sends the external stimulation experiment instruction to the third-party control platform, otherwise, the step of detecting the current stimulation parameter is skipped to;
the third-party control platform controls a corresponding stimulation paradigm according to the received external stimulation experiment instruction to enable the animal behavior analysis experiment device to generate the external stimulation acting on the animal body, and the animal behavior analysis experiment device acquires corresponding second behavior data according to the collected stimulation behavior video of the animal under the external stimulation and sends the second behavior data to the visual control platform;
and the visual control platform analyzes the difference between the second behavior characteristic and the first behavior characteristic of the animal under the external stimulation according to the second behavior data to obtain a difference analysis result, adjusts the external stimulation experiment requirement according to the difference analysis result, sets the second behavior characteristic data corresponding to the second behavior characteristic as the first behavior characteristic data, and skips to the step of detecting the current stimulation parameter.
In another aspect, the present invention provides an animal behavior analysis apparatus of the closed-loop feedback type animal behavior analysis system, the apparatus including:
the behavior video processing unit is used for acquiring a corresponding animal behavior video by the animal behavior analysis experimental device when a request for animal behavior analysis of the animal is received, and performing image processing on the animal behavior video through a preset image processing algorithm to acquire corresponding first behavior data, wherein the animal behavior video is an online video corresponding to the animal in the current activity acquired in real time or a stored offline video;
a behavior feature analysis unit, configured to, when the animal behavior video is an online video, analyze, by the visual control platform, a first behavior feature of the animal in the current activity according to the received first behavior data sent by the animal behavior analysis experiment device, so as to obtain first behavior feature data corresponding to the first behavior feature;
the stimulation parameter detection unit is used for detecting current stimulation parameters, and the current stimulation parameters comprise the first behavior characteristic data, the current stimulation times and the current stimulation interval duration;
the experiment requirement judging unit is used for judging whether the detected current stimulation parameters meet the preset external stimulation experiment requirements or not;
the stimulation instruction generating unit is used for generating a corresponding external stimulation experiment instruction according to the current stimulation parameter and sending the external stimulation experiment instruction to the third-party control platform if the current stimulation parameter is the same as the current stimulation parameter, and otherwise, triggering the stimulation parameter detecting unit to detect the current stimulation parameter;
the external stimulation control unit is used for controlling a corresponding stimulation paradigm by the third-party control platform according to the received external stimulation experiment instruction so that the animal behavior analysis experiment device generates the external stimulation acting on the animal body, and the animal behavior analysis experiment device acquires corresponding second behavior data according to the acquired stimulation behavior video of the animal under the external stimulation and sends the second behavior data to the visual control platform; and
and the experiment requirement adjusting unit is used for analyzing the difference between the second behavior characteristic and the first behavior characteristic of the animal under the external stimulation according to the second behavior data by the visual control platform to obtain a difference analysis result, adjusting the external stimulation experiment requirement according to the difference analysis result, setting the second behavior characteristic data corresponding to the second behavior characteristic as the first behavior characteristic data, and triggering the stimulation parameter detecting unit to detect the current stimulation parameter.
The invention provides a closed loop feedback type animal behavior analysis system, which comprises an animal behavior analysis experimental device, a visual control platform and a third party control platform, the visual control platform analyzes the first behavior characteristics of the animal according to the first behavior data sent by the animal behavior analysis experimental device to obtain corresponding first behavior characteristic data, when the current stimulation parameters containing the first behavior characteristic data meet the requirements of the external stimulation experiment, the third-party control platform gives various stimulations to the animal according to the external stimulation experiment instruction sent by the visual control platform, the stimulated animal performance is fed back to the visual control platform, the corresponding experimental parameter values are changed until the requirements of experimenters are met, therefore, the system cost is reduced, the animal behaviors are accurately analyzed, and research on the relation between the corresponding brain areas or nerves of the animals and the motor behaviors by scientific research personnel is facilitated.
Drawings
Fig. 1 is a schematic structural diagram of a closed-loop feedback type animal behavior analysis system according to an embodiment of the present invention;
FIG. 2 is a flow chart of a method for analyzing animal behavior by closed loop feedback provided in the second embodiment of the present invention;
fig. 3 is a flowchart of an implementation of image processing in a closed-loop feedback type animal behavior analysis method according to a second embodiment of the present invention;
fig. 4 is a flow chart of data storage and display in the closed-loop feedback animal behavior analysis method according to the second embodiment of the present invention;
FIG. 5 is a flow chart of the implementation of the behavior analysis of the animal in the ROI in the closed-loop feedback animal behavior analysis method provided by the second embodiment of the present invention;
fig. 6 is a flow chart of the implementation of the analysis of the animal behavior state in the closed-loop feedback type animal behavior analysis method provided by the second embodiment of the invention; and
fig. 7 is a schematic structural diagram of a closed-loop feedback type animal behavior analysis apparatus according to a third embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The following detailed description of specific implementations of the present invention is provided in conjunction with specific embodiments:
the first embodiment is as follows:
fig. 1 shows a structure of a closed-loop feedback type animal behavior analysis system provided in an embodiment of the present invention, and only a part related to the embodiment of the present invention is shown for convenience of explanation.
The embodiment of the present invention provides a closed-loop feedback type animal behavior analysis system 1, the closed-loop feedback type animal behavior analysis system 1 is suitable for various animals (for example, large animals and small animals), and is suitable for various experimental places (for example, open field, maze, overhead cross, square area, and circular area, etc.), the closed-loop feedback type animal behavior analysis system 1 includes an animal behavior analysis experimental apparatus 11, a visual control platform 12, and a third-party control platform 12, wherein:
the animal behavior analysis experimental device 11 is used for generating external stimuli acting on animals, acquiring corresponding animal behavior videos, and performing image processing on the animal behavior videos through a preset image processing algorithm to obtain corresponding behavior data, wherein the animal behavior videos are online videos corresponding to the animals which are acquired in real time under current activities, or stored offline videos. The visual control platform 12 is connected to the animal experiment device 11, and is configured to analyze the behavior characteristics of the animal according to the received behavior data sent by the animal behavior analysis experiment device 11, obtain behavior characteristic data corresponding to the behavior characteristics, and determine whether the behavior characteristic data meets a preset external stimulation experiment requirement, so as to generate a corresponding external stimulation experiment instruction. The third-party control platform 13 is connected with the animal behavior analysis experiment device 11 and the visual control platform 12, and is used for controlling the corresponding stimulation paradigm according to the external stimulation experiment instruction sent by the received visual control platform 12, so that the animal behavior analysis experiment device 11 generates external stimulation acting on the animal body, and the third-party control platform 13 can be a third-party application program or a third-party hardware control platform.
Preferably, the animal behavior analysis experiment device 11 includes a video capture device (e.g., a wide-angle recording camera) for recording animal behavior videos, and the animal behavior analysis experiment device 11 can obtain animal behavior data only by analyzing the animal behavior videos captured by the video capture device, without depending on various sensors to analyze animal behaviors, thereby reducing the cost of the animal behavior analysis experiment device 11.
Still preferably, the animal behavior analysis experimental apparatus 11 further comprises a light stimulation component, a sound stimulation component, a foot electrical stimulation component and a visual stimulation component, so as to generate external stimulation of one or any combination of light stimulation, sound stimulation, foot electrical stimulation and visual stimulation to the animal, thereby improving the accuracy of animal behavior analysis.
Preferably, the visual control platform 12 is built by using an open-source visual programming language, so that the operability and the interactivity of the closed-loop feedback type animal behavior analysis system 1 are improved, and a user can adjust the external stimulation experiment requirements according to the experiment situation to better analyze the animal behaviors.
The embodiment of the invention provides a closed-loop feedback type animal behavior analysis system, which comprises an animal behavior analysis experimental device, a visual control platform and a third-party control platform, the visual control platform analyzes the first behavior characteristics of the animal according to the first behavior data sent by the animal behavior analysis experimental device to obtain corresponding first behavior characteristic data, when the current stimulation parameters containing the first behavior characteristic data meet the requirements of the external stimulation experiment, the third-party control platform gives various stimulations to the animal according to the external stimulation experiment instruction sent by the visual control platform, the stimulated animal performance is fed back to the visual control platform, the corresponding experimental parameter values are changed until the requirements of experimenters are met, therefore, the system cost is reduced, the animal behaviors are accurately analyzed, and research on the relation between the corresponding brain areas or nerves of the animals and the motor behaviors by scientific research personnel is facilitated.
Example two:
fig. 2 shows an implementation flow of a closed-loop feedback type animal behavior analysis method provided in an embodiment two of the present invention, the closed-loop feedback type animal behavior analysis method is applicable to the closed-loop feedback type animal behavior analysis system in the embodiment one, for convenience of description, only the parts related to the embodiment of the present invention are shown, and details are as follows:
in step S201, when a request for animal behavior analysis of an animal is received, the animal behavior analysis experimental apparatus obtains a corresponding animal behavior video, and performs image processing on the animal behavior video through a preset image processing algorithm to obtain corresponding first behavior data, where the animal behavior video is an online video corresponding to the animal collected in real time under the current activity, or a stored offline video.
The embodiment of the invention is suitable for the closed-loop feedback type animal behavior analysis system in the first embodiment to analyze animal behaviors, and the system comprises an animal behavior analysis experiment device, a visual control platform and a third-party control platform, wherein the animal behavior analysis experiment device is used for generating external stimulation acting on an animal body, acquiring animal behavior videos of the animal corresponding to animal activities, and analyzing the acquired animal behavior videos to obtain corresponding behavior data. The visual control platform is connected with the animal experiment device and used for analyzing the behavior characteristics of the animal according to the received behavior data sent by the animal behavior analysis experiment device to obtain behavior characteristic data corresponding to the behavior characteristics and determining whether the behavior characteristic data meets the preset external stimulation experiment requirement or not so as to generate a corresponding external stimulation experiment instruction. The third-party control platform is connected with the animal behavior analysis experiment device and the visual control platform and used for controlling the corresponding stimulation paradigm according to the received external stimulation experiment instruction sent by the visual control platform, so that the animal behavior analysis experiment device generates external stimulation acting on the animal body.
In the embodiment of the invention, when a request for animal behavior analysis of an animal is received, an animal behavior analysis experimental device acquires an animal behavior video of the animal under current activity, which is acquired in real time by a video acquisition device, or acquires a stored offline animal behavior video according to the request, performs image processing on the animal behavior video through a preset image processing algorithm to obtain corresponding first behavior data, and transmits the obtained first behavior data to a visualization control platform, wherein the first behavior data comprises body centroid coordinates, head centroid coordinates and tail centroid coordinates of the animal.
When the animal behavior video is subjected to image processing through a preset image processing algorithm, as shown in fig. 3, the image processing on the animal behavior video is preferably realized through the following steps:
in step S301, the animal behavior video is subjected to distortion removal processing, so that the measurement accuracy of the processed animal behavior video is improved, and a corresponding first video is obtained;
in step S302, color balance processing is performed on the first video to obtain a second video;
in step S303, performing graying processing on the second video to distinguish the animal in the second video from the surrounding environment where the animal is located, so as to obtain a corresponding third video;
in step S304, the surrounding environment of the animal is cut from the third video by an adaptive threshold algorithm to obtain a corresponding fourth video, so that the animal behavior can be better analyzed by the fourth video;
in step S305, recognizing the contour of the animal in the fourth video according to a preset image contour recognition algorithm;
in step S306, the identified animal contour and the environmental noise are distinguished by a binary region analysis algorithm and a maximized binary region algorithm, so that the obtained animal contour is clearer and more accurate;
in step S307, body centroid coordinates, head centroid coordinates, and tail centroid coordinates of the animal are calculated from the animal contour.
Therefore, the animal behavior video is subjected to image processing through the steps S301-S307, so that the processed animal behavior video has a pure background and clear animal outline, and the accuracy of the first behavior data is improved.
After image processing is carried out on the animal behavior video through a preset image processing algorithm to obtain corresponding first behavior data, preferably, when the animal behavior video is an offline video, the motion track characteristics of the animal are extracted according to the first behavior data, and the track thermodynamic diagram of the animal in a preset experiment site is drawn according to the extracted motion track characteristics to analyze the frequency of the animal appearing in the experiment site, so that the diversity of animal behavior analysis is improved.
In step S202, when the animal behavior video is an online video, the visualization control platform analyzes the first behavior feature of the animal in the current activity according to the received first behavior data sent by the animal behavior analysis experimental apparatus, so as to obtain first behavior feature data corresponding to the first behavior feature.
In the embodiment of the present invention, after the visualization control platform receives the first behavior data of the animal, the first behavior feature (for example, the movement speed, the movement acceleration, the head deflection angle, and the like) of the animal in the current activity is analyzed, so as to obtain the first behavior feature data corresponding to the first behavior feature, where the first behavior feature data refers to a specific numerical value corresponding to each first behavior feature. Specifically, the movement speed of the animal and the acceleration of the movement of the animal are calculated according to the coordinates of the mass center of the animal body, the real-time track of the movement of the animal is obtained, the head deflection angle of the animal is calculated according to the coordinates of the mass center of the head of the animal, and the tail deflection angle of the animal is calculated according to the coordinates of the mass center of the tail of the animal, so that the accuracy of the first behavior characteristic data is improved.
In step S203, a current stimulation parameter is detected, where the current stimulation parameter includes the first behavior feature data, the current stimulation frequency, and the current stimulation interval duration.
In an embodiment of the present invention, when the visual control platform detects the current stimulation parameters, the current stimulation parameters include the first behavior feature data (e.g., the current speed value of animal movement, the current acceleration value, the current deflection angle of head, etc.), the current stimulation times, and the current stimulation interval duration.
After the current stimulation parameters are detected, as shown in fig. 4, preferably,
in step S401, a preset data saving instruction is detected to determine whether the data saving instruction is triggered.
In the embodiment of the present invention, the data saving command may be triggered by a user through an interactive interface (e.g., a data saving button), or may be automatically triggered through a preset data saving time (e.g., AM 11:00) or a preset data saving period (e.g., every 2 hours).
In step S402, when the data saving instruction is detected, the current stimulation parameters, the external stimulation experiment requirement, the animal behavior video, and the image-processed animal behavior video are stored.
In the embodiment of the invention, the current stimulation parameters, the external stimulation experiment instruction, the animal behavior video and the animal behavior video after image processing are correspondingly stored, so that a basis is provided for animal behavior analysis in an off-line state. Specifically, the body barycentric coordinate, the head barycentric coordinate, the tail barycentric coordinate, the movement speed/acceleration, the head deflection angle, the tail deflection angle, the movement distance, the movement time, the times and time of entering the interested area (within a certain period of time or the whole experiment time), the times and time of interacting with the animal at the fixed position (within a certain period of time or the whole experiment time), the coordinates when the external stimulus is triggered, the time, the times and time of Freezzing and Flate (within a certain period of time or the whole experiment time), and the non-video data such as other various animal behavior variables are stored together in an excel or txt, the video data of the animal behavior and the video data of the animal behavior after image processing are respectively and independently stored, and the non-video data and the video data can be automatically and completely stored, the data can be selectively stored according to the needs of the user, so that the user can conveniently view the data later, or perform off-line analysis on animal behaviors.
Therefore, the step S401 to the step S402 realize off-line storage of experimental data, and the convenience of animal behavior analysis in an off-line state is improved.
After the current stimulation parameter is detected, preferably, the current stimulation parameter and the preset stimulation condition trigger mark are displayed on a preset graphical user interface in real time on line, and a behavior video and related information of the animal under the current activity or the current external stimulation are displayed on a preset display device.
In the embodiment of the invention, current stimulation parameters (such as real-time track of an animal, movement speed of the animal, head deflection angle of the animal, movement acceleration of the animal and the like) and a preset stimulation condition trigger mark are displayed on a preset Graphic User Interface (GUI) in real time on line, and a behavior video and related information (such as speed, topological structure, moving direction and the like of a mouse under visual stimulation) of the animal under the current activity or the current external stimulation are displayed on a preset display device (such as a display screen and an LED giant screen), so that the intuitiveness and convenience of animal behavior analysis are improved, wherein the stimulation condition trigger mark indicates that when animal behavior meets the condition set by a researcher, the researcher is reminded in real time through a mark to meet the set external stimulation experiment requirement, the mark may be a circle or a color change, and is not fixed.
In step S204, it is determined whether the detected current stimulation parameter meets a preset external stimulation experiment requirement.
In the embodiment of the present invention, the detected current stimulation parameter is compared with the preset external stimulation experiment requirement, and whether the external stimulation experiment requirement is met is determined, if yes, step S205 is executed, otherwise, step S203 is skipped, wherein, the external stimulation experiment requirement includes a speed limit threshold, a distance limit threshold, a time limit threshold, and a stimulation number limit threshold, and the external stimulation experiment requirement may be one of the speed limit threshold, the distance limit threshold, the time limit threshold, and the stimulation number limit threshold, or may also be any combination of the speed limit threshold, the distance limit threshold, the time limit threshold, and the stimulation number limit threshold, for example, determining whether the current speed value of the animal movement reaches the speed limit threshold, or judging whether the current speed value and the current stimulation times of the animal movement meet the speed limit threshold and the stimulation times limit threshold simultaneously.
Before judging whether the detected current stimulation parameters meet the preset external stimulation experiment requirements or not, preferably, the visual control platform receives adjustment (for example, adjustment speed limit threshold) of corresponding experiment parameters of the user to the external stimulation experiment requirements according to the animal behavior video, and sets the external stimulation experiment requirements according to the received and adjusted corresponding experiment parameters, so that the rationality of the external stimulation experiment requirements is improved.
In step S205, the visual control platform generates a corresponding external stimulation experiment instruction according to the current stimulation parameter, and sends the external stimulation experiment instruction to the third-party control platform.
In the embodiment of the present invention, when it is determined that the current stimulation parameter meets the external stimulation experiment requirement, the visual control platform generates a corresponding external stimulation experiment instruction according to the trigger condition meeting the external stimulation experiment requirement, the trigger condition meeting the external stimulation experiment requirement may be one (that is, the corresponding external stimulation experiment instruction is generated if the one trigger condition is met) or multiple (that is, the corresponding external stimulation experiment instruction is generated if multiple trigger conditions (for example, three trigger conditions of speed, distance, and two-time stimulation time interval are met simultaneously) are met), the external stimulation experiment instruction corresponds to a certain external stimulation acting on an animal, and the external stimulation experiment instruction is sent to a third-party control platform, the external stimulation experiment instruction includes the position, the direction vector, and the external stimulation mode, the external stimulation mode includes an acoustic stimulation mode, a mode, and a mode, and a mode, a mode, The light stimulation, the electric stimulation and the visual stimulation modes, and the corresponding external stimulation generated by each external stimulation mode is independent. As an example, when the movement speed of the animal reaches the primary speed limit threshold, the visual control platform generates a light stimulation instruction according to the trigger condition, or when the movement speed of the animal reaches the secondary speed limit threshold and the distance between the animal and a specific point reaches a preset distance threshold, the visual control platform generates a sound stimulation instruction according to the trigger condition, and the like.
In step S206, the third-party control platform controls the corresponding stimulation paradigm according to the received external stimulation experiment instruction, so that the animal behavior analysis experiment device generates an external stimulation acting on the animal, and the animal behavior analysis experiment device obtains corresponding second behavior data according to the collected stimulation behavior video of the animal under the external stimulation, and sends the second behavior data to the visual control platform.
In the embodiment of the invention, the third-party control platform controls a stimulation paradigm corresponding to an external stimulation experiment instruction, the stimulation paradigm corresponds to sound, light, electricity, vision and other stimulation modes required by an animal behavior analysis experiment, so that the animal behavior analysis experiment device generates corresponding external stimulation acting on an animal body, under the external stimulation, the animal behavior analysis experiment device acquires corresponding second behavior data according to the acquired stimulation behavior video and sends the second behavior data to the visual control platform to realize the analysis of animal behavior of the animal under the external stimulation, wherein the external stimulation comprises visual stimulation, light stimulation, sound stimulation and plantar electrical stimulation, and the most common visual stimulation is looming stimulation.
In step S207, the visualization control platform analyzes the difference between the second behavior feature and the first behavior feature of the animal under the external stimulus according to the second behavior data to obtain a difference analysis result, adjusts the external stimulus experiment requirement according to the difference analysis result, and sets the second behavior feature data corresponding to the second behavior feature as the first behavior feature data.
In the embodiment of the present invention, the visualization control platform analyzes a difference between a second behavior feature of the animal under the external stimulus and the first behavior feature according to the second behavior data (for example, analyzes a difference between the second behavior feature of the animal under the light stimulus and the first behavior feature generated by the previous electrical stimulus, or a difference between the second behavior feature and the first behavior feature without the external stimulus), obtains a difference analysis result (for example, a change in speed, a change in acceleration, a change in the movement direction of the animal, etc.), adjusts the external stimulus experiment requirement according to the difference analysis result (for example, adjusts a threshold of stimulation times, a threshold of stimulation interval duration, etc.), sets the second behavior feature data corresponding to the second behavior feature as the first behavior feature data, and moves to step S203 to continue to detect the current stimulation parameter for the next round of stimulus experiment, or a characterization of the behavior of the next round of animals.
After the second behavior feature data corresponding to the second behavior feature is set as the first behavior feature data, as shown in fig. 5, preferably,
in step S501, it is determined whether the animal enters a preset region of interest according to the first behavior feature data.
In the embodiment of the invention, the distance from the animal in the first behavior feature data to the specific point is compared with a preset specific point distance threshold value, and whether the animal enters a Region of interest (ROI) is determined according to the comparison result.
In step S502, when it is determined that the animal enters the region of interest, the time and the number of times that the animal enters the region of interest are counted, or the time and the number of times that the animal enters the region of interest within a preset first time period are counted.
Therefore, the animal behavioral analysis of the ROI is realized through the steps S501-S502, and the multifaceted animal behavioral analysis is improved.
After obtaining the difference analysis result, as shown in fig. 6, preferably,
in step S601, the behavior state of the animal under the external stimulus is analyzed according to the difference analysis result and the second behavior feature data corresponding to the second behavior feature, so as to obtain a corresponding behavior state analysis result, where the behavior state includes a stiff state, a fast running state, and other states.
In the embodiment of the present invention, the behavior state of the animal under the external stimulus is analyzed according to the difference analysis result and the second behavior feature data (for example, the movement speed and the movement time of the animal) corresponding to the second behavior feature, so as to obtain a corresponding behavior state analysis result, where the behavior state includes a stiff state (speeding state), a fast running state (flash state), and other states (i.e., neither the speeding state nor the flash state).
In step S602, the time and the number of times that the animal is in the stiff state/fast running state are counted or the time and the number of times that the animal is in the stiff state/fast running state for a preset second time period are counted according to the state analysis result.
Therefore, the behavioral analysis of the animal free/flat state is realized through the steps S601-S602, so that scientific researchers can conveniently understand the relationship between the instinct fear and the conditional fear of the animal, and further help is provided for researching the neural loop and the brain area related to the instinct fear and the conditional fear.
In the embodiment of the present invention, it is further preferable that the number of times and the time of contact between the animal and one or more other animals in the preset region of interest are analyzed according to the animal behavior video, wherein the animal behavior video includes an online video acquired in real time and a stored offline video, so that the versatility of animal behavior analysis is further improved by analyzing the interaction behavior between the animal and the animal.
In the embodiment of the invention, the visual control platform analyzes the first behavioral characteristics of the animal according to the first behavioral data sent by the animal behavior analysis experimental device to obtain corresponding first behavioral characteristic data, when the current stimulation parameters including the first behavioral characteristic data meet the requirements of external stimulation experiments, the third-party control platform gives various stimulations to the animal according to the external stimulation experiment instructions sent by the visual control platform, and the stimulated animal performance is fed back to the visual control platform to change corresponding experiment parameter values until the requirements of experimenters are met, so that the accurate analysis of animal behaviors is realized, the comprehensiveness, diversity and accuracy of animal behavior analysis are improved, and the research on the relationship between corresponding brain areas or nerves of the animal and the motor behaviors by scientific researchers is facilitated.
Example three:
fig. 7 shows a structure of a closed-loop feedback type animal behavior analysis apparatus provided in a third embodiment of the present invention, which is applied to a closed-loop feedback type animal behavior analysis system in the first embodiment, and for convenience of illustration, only a part related to the first embodiment of the present invention is shown, where the closed-loop feedback type animal behavior analysis apparatus includes:
the behavior video processing unit 71 is configured to, when a request for animal behavior analysis of an animal is received, acquire a corresponding animal behavior video by the animal behavior analysis experimental apparatus, and perform image processing on the animal behavior video through a preset image processing algorithm to obtain corresponding first behavior data, where the animal behavior video is an online video corresponding to the animal acquired in real time under the current activity, or a stored offline video;
the behavior feature analysis unit 72 is configured to, when the animal behavior video is an online video, analyze the first behavior feature of the animal in the current activity according to the received first behavior data sent by the animal behavior analysis experimental apparatus, and obtain first behavior feature data corresponding to the first behavior feature;
a stimulation parameter detection unit 73, configured to detect a current stimulation parameter, where the current stimulation parameter includes first behavior feature data, a current stimulation frequency, and a current stimulation interval duration;
an experiment requirement judging unit 74, configured to judge whether the detected current stimulation parameter meets a preset external stimulation experiment requirement;
the stimulation instruction generating unit 75 is configured to, if yes, the visualization control platform generates a corresponding external stimulation experiment instruction according to the current stimulation parameter, and sends the external stimulation experiment instruction to the third-party control platform, and otherwise, the stimulation parameter detecting unit 73 is triggered to perform detection on the current stimulation parameter;
the external stimulation control unit 76 is used for controlling the corresponding stimulation paradigm by the third-party control platform according to the received external stimulation experiment instruction, so that the animal behavior analysis experiment device generates external stimulation acting on the animal body, and acquires corresponding second behavior data according to the collected stimulation behavior video of the animal under the external stimulation, and sends the second behavior data to the visual control platform; and
and the experiment requirement adjusting unit 77 is configured to analyze, by the visual control platform, a difference between the second behavior characteristic and the first behavior characteristic of the animal under the external stimulus according to the second behavior data, obtain a difference analysis result, adjust the external stimulus experiment requirement according to the difference analysis result, set the second behavior characteristic data corresponding to the second behavior characteristic as the first behavior characteristic data, and trigger the stimulus parameter detecting unit 73 to perform detection on the current stimulus parameter.
Preferably, the closed-loop feedback type animal behavior analysis apparatus according to the embodiment of the present invention further includes:
the data saving device comprises a saving instruction detection unit, a data saving instruction processing unit and a data saving instruction processing unit, wherein the saving instruction detection unit is used for detecting a preset data saving instruction so as to determine whether the data saving instruction is triggered or not; and
and the data storage unit is used for storing the current stimulation parameters, the external stimulation experiment requirements, the animal behavior videos and the animal behavior videos after image processing when the data storage instruction is detected.
Preferably, the closed-loop feedback type animal behavior analysis apparatus according to the embodiment of the present invention further includes:
and the thermodynamic diagram drawing unit is used for extracting the motion track characteristics of the animal according to the first behavior data when the animal behavior video is an offline video, and drawing the track thermodynamic diagram of the animal in a preset experimental site according to the extracted motion track characteristics.
Preferably, the closed-loop feedback type animal behavior analysis apparatus according to the embodiment of the present invention further includes:
the area state determining unit is used for determining whether the animal enters a preset area of interest or not according to the first behavior characteristic data; and
the first parameter statistical unit is used for counting the time and the times of the animal entering the region of interest when the animal is determined to enter the region of interest, or counting the time and the times of the animal entering the region of interest within a preset first time period.
Preferably, the closed-loop feedback type animal behavior analysis apparatus according to the embodiment of the present invention further includes:
the behavior state analysis unit is used for analyzing the behavior state of the animal under the external stimulation according to the difference analysis result and second behavior characteristic data corresponding to the second behavior characteristics to obtain a corresponding behavior state analysis result, wherein the behavior state comprises a stiff state, a rapid running state and other states; and
and the second parameter counting unit is used for counting the time and the times of the animal in the stiff state/the fast running state according to the state analysis result, or counting the time and the times of the animal in the stiff state/the fast running state in a preset second time period.
In the embodiment of the present invention, each unit of the closed-loop feedback type animal behavior analysis apparatus may be implemented by a corresponding hardware or software unit, and each unit may be an independent software or hardware unit, or may be integrated into a software or hardware unit, which is not limited herein. Specifically, the implementation of each unit can refer to the description of the foregoing method embodiment, and is not repeated herein.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (10)
1. A method of analyzing animal behavior, comprising the steps of:
when a request for animal behavior analysis of the animal is received, the animal behavior analysis experimental device obtains a corresponding animal behavior video, and performs image processing on the animal behavior video through a preset image processing algorithm to obtain corresponding first behavior data, wherein the animal behavior video is an online video corresponding to the animal in the current activity acquired in real time or a stored offline video;
when the animal behavior video is an online video, analyzing the first behavior characteristics of the animal under the current activity by a visual control platform according to the received first behavior data sent by the animal behavior analysis experiment device to obtain first behavior characteristic data corresponding to the first behavior characteristics;
detecting current stimulation parameters, wherein the current stimulation parameters comprise the first behavior characteristic data, the current stimulation times and the current stimulation interval duration;
judging whether the detected current stimulation parameters meet preset external stimulation experiment requirements or not;
if so, the visual control platform generates a corresponding external stimulation experiment instruction according to the current stimulation parameter and sends the external stimulation experiment instruction to a third-party control platform, otherwise, the step of detecting the current stimulation parameter is skipped;
the third-party control platform controls a corresponding stimulation paradigm according to the received external stimulation experiment instruction to enable the animal behavior analysis experiment device to generate the external stimulation acting on the animal body, and the animal behavior analysis experiment device acquires corresponding second behavior data according to the collected stimulation behavior video of the animal under the external stimulation and sends the second behavior data to the visual control platform;
and the visual control platform analyzes the difference between the second behavior characteristic and the first behavior characteristic of the animal under the external stimulation according to the second behavior data to obtain a difference analysis result, adjusts the external stimulation experiment requirement according to the difference analysis result, sets the second behavior characteristic data corresponding to the second behavior characteristic as the first behavior characteristic data, and skips to the step of detecting the current stimulation parameter.
2. The method of claim 1, wherein after the step of detecting the current stimulation parameter, the method further comprises:
detecting a preset data storage instruction to determine whether the data storage instruction is triggered;
and when the data storage instruction is detected, storing the current stimulation parameter, the external stimulation experiment requirement, the animal behavior video and the animal behavior video after image processing.
3. The method of claim 1, wherein after the step of performing image processing on the animal behavior video through a preset image processing algorithm to obtain corresponding first behavior data, the method further comprises:
when the animal behavior video is an offline video, extracting the motion track characteristics of the animal according to the first behavior data, and drawing a track thermodynamic diagram of the animal in a preset experimental site according to the extracted motion track characteristics.
4. The method of claim 1, wherein after the step of setting second behavior feature data corresponding to the second behavior feature as the first behavior feature data, the method further comprises:
determining whether the animal enters a preset region of interest or not according to the first behavior feature data;
when the animal is determined to enter the region of interest, counting the time and the times of the animal entering the region of interest, or counting the time and the times of the animal entering the region of interest within a preset first time period.
5. The method of claim 1, wherein after the step of obtaining the difference analysis results, the method further comprises:
analyzing the behavior state of the animal under the external stimulus according to the difference analysis result and second behavior feature data corresponding to the second behavior feature to obtain a corresponding behavior state analysis result, wherein the behavior state comprises a stiff state, a rapid running state and other states;
and according to the state analysis result, counting the time and the times of the animal in the stiff state/fast running state, or counting the time and the times of the animal in the stiff state/fast running state in a preset second time period.
6. An animal behavior analysis device, characterized in that the device comprises:
the behavior video processing unit is used for acquiring a corresponding animal behavior video by the animal behavior analysis experimental device when a request for animal behavior analysis of the animal is received, and performing image processing on the animal behavior video through a preset image processing algorithm to acquire corresponding first behavior data, wherein the animal behavior video is an online video corresponding to the animal in the current activity acquired in real time or a stored offline video;
the behavior feature analysis unit is used for analyzing the first behavior feature of the animal under the current activity according to the received first behavior data sent by the animal behavior analysis experimental device by the visual control platform when the animal behavior video is an online video to obtain first behavior feature data corresponding to the first behavior feature;
the stimulation parameter detection unit is used for detecting current stimulation parameters, and the current stimulation parameters comprise the first behavior characteristic data, the current stimulation times and the current stimulation interval duration;
the experiment requirement judging unit is used for judging whether the detected current stimulation parameters meet the preset external stimulation experiment requirements or not; the stimulation instruction generating unit is used for generating a corresponding external stimulation experiment instruction according to the current stimulation parameter through the visual control platform and sending the external stimulation experiment instruction to a third-party control platform, and otherwise, triggering the stimulation parameter detecting unit to detect the current stimulation parameter;
the external stimulation control unit is used for controlling a corresponding stimulation paradigm by the third-party control platform according to the received external stimulation experiment instruction so that the animal behavior analysis experiment device generates the external stimulation acting on the animal body, and the animal behavior analysis experiment device acquires corresponding second behavior data according to the acquired stimulation behavior video of the animal under the external stimulation and sends the second behavior data to the visual control platform; and
and the experiment requirement adjusting unit is used for analyzing the difference between the second behavior characteristic and the first behavior characteristic of the animal under the external stimulation according to the second behavior data by the visual control platform to obtain a difference analysis result, adjusting the external stimulation experiment requirement according to the difference analysis result, setting the second behavior characteristic data corresponding to the second behavior characteristic as the first behavior characteristic data, and triggering the stimulation parameter detecting unit to detect the current stimulation parameter.
7. The apparatus of claim 6, wherein the apparatus further comprises:
the data saving device comprises a saving instruction detection unit, a data saving instruction processing unit and a data saving instruction processing unit, wherein the saving instruction detection unit is used for detecting a preset data saving instruction so as to determine whether the data saving instruction is triggered or not; and
and the data storage unit is used for storing the current stimulation parameters, the external stimulation experiment requirements, the animal behavior videos and the animal behavior videos after image processing when the data storage instruction is detected.
8. The apparatus of claim 6, wherein the apparatus further comprises:
and the thermodynamic diagram drawing unit is used for extracting the motion track characteristics of the animal according to the first behavior data when the animal behavior video is an offline video, and drawing the track thermodynamic diagram of the animal in a preset experimental site according to the extracted motion track characteristics.
9. The apparatus of claim 6, wherein the apparatus further comprises:
the area state determining unit is used for determining whether the animal enters a preset area of interest or not according to the first behavior feature data; and
the first parameter statistical unit is used for counting the time and the times of the animal entering the region of interest when the animal is determined to enter the region of interest, or counting the time and the times of the animal entering the region of interest within a preset first time period.
10. The apparatus of claim 6, wherein the apparatus further comprises:
the behavior state analysis unit is used for analyzing the behavior state of the animal under the external stimulus according to the difference analysis result and second behavior characteristic data corresponding to the second behavior characteristic to obtain a corresponding behavior state analysis result, wherein the behavior state comprises a stiff state, a fast running state and other states; and
and the second parameter counting unit is used for counting the time and the times of the animal in the stiff state/fast running state according to the state analysis result, or counting the time and the times of the animal in the stiff state/fast running state in a preset second time period.
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