CN103805566A - 人脂肪间充质干细胞三维培养分化成神经样干细胞的方法 - Google Patents
人脂肪间充质干细胞三维培养分化成神经样干细胞的方法 Download PDFInfo
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- CN103805566A CN103805566A CN201310737266.XA CN201310737266A CN103805566A CN 103805566 A CN103805566 A CN 103805566A CN 201310737266 A CN201310737266 A CN 201310737266A CN 103805566 A CN103805566 A CN 103805566A
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- human adipose
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total RNA | 10ng-2μg |
oligo(dT)15(50μM) | 1μL |
random6mers(50μM) | 1μL |
10mM dNTP mix | 1μL |
无核酸酶水 | to18μL |
5×RT Buffer | 5μL |
M-MLV Reverase Transcriptase | 1μL |
RNase Inhibitor(40U/μL) | 1μL |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN106635993A (zh) * | 2015-10-30 | 2017-05-10 | 广州思丹福生物科技有限公司 | 一种诱导人胎盘绒膜间充质干细胞分化为神经样干细胞的方法 |
CN113373111A (zh) * | 2021-06-25 | 2021-09-10 | 贺巾帼 | 一种培养脂肪间充质干细胞球状体的方法 |
Citations (4)
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CN101831401A (zh) * | 2010-04-23 | 2010-09-15 | 天津昂赛细胞基因工程有限公司 | 体外诱导间充质干细胞分化为神经干细胞的方法 |
CN102836016A (zh) * | 2011-06-20 | 2012-12-26 | 中山大学附属第一医院 | 促进周围神经移植后神经再生的植入式可降解装置 |
CN103013917A (zh) * | 2012-11-30 | 2013-04-03 | 陆华 | 人羊膜间充质干细胞分化为神经元样细胞的诱导方法 |
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Patent Citations (4)
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CN101831401A (zh) * | 2010-04-23 | 2010-09-15 | 天津昂赛细胞基因工程有限公司 | 体外诱导间充质干细胞分化为神经干细胞的方法 |
CN102836016A (zh) * | 2011-06-20 | 2012-12-26 | 中山大学附属第一医院 | 促进周围神经移植后神经再生的植入式可降解装置 |
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Non-Patent Citations (2)
Title |
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于志永 等: ""不同种植密度对大鼠脂肪间充质干细胞三维培养下成软骨能力的影响"", 《中国组织工程研究与临床康复》 * |
耿涛 等: ""骨髓间充质干细胞三维培养—构建工程组织"", 《科技导报》 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106635993A (zh) * | 2015-10-30 | 2017-05-10 | 广州思丹福生物科技有限公司 | 一种诱导人胎盘绒膜间充质干细胞分化为神经样干细胞的方法 |
CN113373111A (zh) * | 2021-06-25 | 2021-09-10 | 贺巾帼 | 一种培养脂肪间充质干细胞球状体的方法 |
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