CN103804468A - Preparation method of nano pearl proteins - Google Patents
Preparation method of nano pearl proteins Download PDFInfo
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- CN103804468A CN103804468A CN201410055730.1A CN201410055730A CN103804468A CN 103804468 A CN103804468 A CN 103804468A CN 201410055730 A CN201410055730 A CN 201410055730A CN 103804468 A CN103804468 A CN 103804468A
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Abstract
The invention relates to a preparation method of nano pearl proteins. The preparation method comprises the following steps: crushing pearls into pearl micropowder with the average grain size of 1-4 microns, carrying out acidolysis, filtering, washing the filter residues by purified water to obtain coarse pearl proteins, adding a dispersant into the coarse pearl proteins to grind, and carrying out ultrasonic concussion in the period; and concentrating the grinded liquid by a membrane, and heating, sterilizing and storing to obtain the nano pearl proteins. The preparation method provided by the invention is simple in process, organically combines the nano grinding technology with the emulsifying and dispersing technology, and further enhances the emulsifying and dispersing effect by means of ultrasonic waves, so that the pearl protein particles effectively reach nanoscale. The pure nature and integrity of the pearl protein component can be maintained by pure physical processing, and the nano pearl proteins are uniform in particle and are easily assimilated by human body and further by skins, so that the nano pearl proteins are suitable for being used as an additive of medicines, nutrients and skin care products.
Description
Technical field
The present invention relates to a kind of pearl powder processing technique field medical or used for cosmetic, particularly a kind of preparation method of nano-pearl albumen.
Background technology
Pearl is a kind of famous and precious Chinese medicinal materials, have calm the nerves, effect of improving eyesight, removing toxic substances and beauty and health care.Modern science analysis shows, pearl is containing abundant pearl protein, amino acid, 20 various trace elements, porphyrin and abundant calcium class.Wherein pearl protein is cosmetic good merchantable brand, and external application is covered on skin, has the nutrition skin effects such as moisturizing, nutrition skin, whitening.
At present, the extraction of pearl protein mainly adopts acid-soluble method, by dissolving by edible acid after crushing pearl, filters, and filter residue is pearl protein by the washes clean of purifying waste water.Adopt acid-soluble method to extract pearl protein particle larger, the more difficult absorption of outer used time skin, is difficult to reach desired effects.
Summary of the invention
The preparation method who the object of the invention is to provide for the deficiencies in the prior art a kind of nano-pearl albumen, can be machined to pearl protein nano level fineness effectively, can guarantee pure natural property and the integrity of pearl protein composition, is suitable for makeup and uses.
The technical solution adopted in the present invention:
A preparation method for nano-pearl albumen, its concrete steps are as follows:
1, by broken pearl to 300 order fineness, then pearl powder being made to median size through comminution by gas stream is the Margarita micropowder of 1～4 micron, then by edible acid, Margarita micropowder is dissolved completely, filters, filter residue is thick pearl protein by the washes clean of purifying waste water.
Described edible acid is 5～25% alimentary acetic acids.Described filtration is the manual press filtration of filter cloth of using with plate-and-frame filter press.
2, in thick pearl protein, add dispersion agent, take the add-on of material dry weight basis dispersion agent as 0.2～4% of thick pearl protein, then grind 60～600 minutes with nano-level grinder, during this time with supersonic cleaning machine concussion 20～100min, pearl protein particle is fully uniformly dispersed, is conducive to improve mill efficiency.
Described dispersion agent is polysorbas20 or Sodium hexametaphosphate 99 liquid.
Described nano-level grinder refers to horizontal nanometer sand mill, and zirconium oxide balls specification used, 0.1～0.8 millimeter of particle diameter, is ground rotating speed between 200～3000 revs/min.
3, the liquid after grinding separates the liquid of 1000 molecular weight with membrane separation apparatus, (molecular weight cut-off is greater than 1000 daltonian compositions to obtain being greater than the solidliquid mixture of 1000 molecular weight, play and remove salinity, the effect of concentrated solid-liquid material nano-pearl albumen), and this mixture is concentrated to solid-to-liquid ratio is 0.01～0.1:1, then heat-sterilization is preserved and is obtained nano-pearl albumen.Detect through laser particle analyzer, median size is less than 100 nanometers.
Described membrane separation apparatus refers to middle control cellulosic ultrafiltration membrane separation apparatus or Ceramic excessive filtration membrane separation apparatus.
Technique of the present invention is simple, nano-milled technology and emulsification, dispersion technology are organically combined, and utilize ultrasonic wave further to strengthen emulsification dispersion effect, can effectively make pearl protein particle reach nano level, adopt pure Physical Processing can retain pure natural property and the integrity of pearl protein composition, the uniform particles of gained nano-pearl albumen, is easy to digested, also be easily absorbed by the skin, be suitable for medicine, nutrition and skin care product and use as additive.
Embodiment
Below in conjunction with embodiment, the specific embodiment of the present invention is described in further detail.Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.The experimental technique of unreceipted actual conditions in the following example, conventionally according to normal condition, or the condition of advising according to manufacturer.
Embodiment mono-
Get pearl grain 10kg through crusher in crushing, be crushed to 300 order particles through supper micron mill, then pulverize through micronizer mill, obtaining median size is 1-4 μ m pearl powder 7.83kg.
Soak this 7.83kg pearl powder 72 hours with 15% alimentary acetic acid solution 100kg, pearl powder fully dissolves, and with filter-cloth filtering, obtains filter residue, with the repetitive scrubbing filter residue of purifying waste water between Ph5-8.Obtain filter residue 0.310kg(moisture), get 10g and dry detection, must be dried thing 4.723g, calculate this filter residue solid content 47.23%.
Filter residue adds purifies waste water to 10kg, adds 1g polysorbas20, and 3g Sodium hexametaphosphate 99 stirs and then grinds with the horizontal nanometer sand mill of KLZr-3, rotating speed 2000r/min, and abrading-ball 0.2mm, grinds 6h.After grinding 1h, emit lapping liquid, the ultrasonic concussion of supersonic cleaning machine 30min for lapping liquid, then continues to grind 5h.Sampling 1ml detects through Ma Erwen 2000 laser particle analyzers, d0.1=0.038 μ m, and d0.5=0.087 μ m, d0.9=0.183 μ m, reaches the requirement of Nano grade.
Adopt Chu Maer lab membrane phase study machine concentrating and desalinating, filter core molecular weight cut-off 1000 dalton, pressure 0.10mpa, in concentration process, gradation adds and purifies waste water, and each 3kg, adds 30kg altogether, is concentrated to 2kg.Heat 100 degrees Celsius of sterilizations in 10 minutes for subsequent use.Adopt Keyes's nitriding to measure, concentrated solution is containing total protein 5.12%.Embodiment bis-
Get pearl grain 15kg through crusher in crushing, be crushed to 300 order particles through supper micron mill, then pulverize through micronizer mill, obtaining median size is 1-4 μ m pearl powder 12.6kg.
Soak this 12.6kg pearl powder 72 hours with 15% alimentary acetic acid solution 150kg, pearl powder fully dissolves, and with filter-cloth filtering, obtains filter residue, with the repetitive scrubbing filter residue of purifying waste water between Ph5-8.Obtain filter residue 0.453kg(moisture), get 10g and dry detection, must be dried thing 4.693g, calculate this filter residue solid content 46.93%.
Filter residue adds purifies waste water to 15kg, adds 1.5g polysorbas20, and 4.5g Sodium hexametaphosphate 99 stirs and then grinds with the horizontal nanometer sand mill of KLZr-3, rotating speed 2000r/min, and abrading-ball 0.2mm, grinds 9h.After grinding 2h, emit lapping liquid, the ultrasonic concussion of supersonic cleaning machine 30min for lapping liquid, then continues to grind 7h.Sampling 1ml detects through Ma Erwen 2000 laser particle analyzers, d0.1=0.031 μ m, and d0.5=0.082 μ m, d0.9=0.163 μ m, reaches the requirement of Nano grade.
Adopt Chu Maer lab membrane phase study machine concentrating and desalinating, filter core molecular weight cut-off 1000 dalton, pressure 0.10mpa, in concentration process, gradation adds and purifies waste water, and each 3kg, adds 45kg altogether, is concentrated to 3kg.Heat 100 degrees Celsius of sterilizations in 10 minutes for subsequent use.Adopt Keyes's nitriding to measure, concentrated solution is containing total protein 5.32%.Embodiment tri-
Get pearl grain 20kg through crusher in crushing, be crushed to 300 order particles through supper micron mill, then pulverize through micronizer mill, obtaining median size is 1-4 μ m pearl powder 17.4kg.
Soak this 17.4kg pearl powder 72 hours with 15% alimentary acetic acid solution 200kg, pearl powder fully dissolves, and with filter-cloth filtering, obtains filter residue, with the repetitive scrubbing filter residue of purifying waste water between Ph5-8.Obtain filter residue 0.680kg(moisture), get 10g and dry detection, must be dried thing 4.771g, calculate this filter residue solid content 47.771%.
Filter residue adds purifies waste water to 20kg, adds 2g polysorbas20, and 6g Sodium hexametaphosphate 99 stirs and then grinds with the horizontal nanometer sand mill of KLZr-3, rotating speed 2000r/min, and abrading-ball 0.2mm, grinds 11h.After grinding 1h, emit lapping liquid, the ultrasonic concussion of supersonic cleaning machine 30min for lapping liquid, then continues to grind 10h.Sampling 1ml detects through Ma Erwen 2000 laser particle analyzers, d0.1=0.035 μ m, and d0.5=0.092 μ m, d0.9=0.167 μ m, reaches the requirement of Nano grade.
Adopt Chu Maer lab membrane phase study machine concentrating and desalinating, filter core molecular weight cut-off 1000 dalton, pressure 0.10mpa, in concentration process, gradation adds and purifies waste water, and each 3kg, adds 60kg altogether, is concentrated to 4kg.Heat 100 degrees Celsius of sterilizations in 10 minutes for subsequent use.Adopt Keyes's nitriding to measure, concentrated solution is containing total protein 5.10%.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, do not departing under the prerequisite of the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (5)
1. a preparation method for nano-pearl albumen, is characterized in that, its concrete steps are as follows:
1), by broken pearl to 300 order fineness, then pearl powder being made to median size through comminution by gas stream is the Margarita micropowder of 1～4 micron, then by edible acid, Margarita micropowder is dissolved completely, filters, filter residue is thick pearl protein by the washes clean of purifying waste water;
2), in thick pearl protein, add dispersion agent, take the add-on of material dry weight basis dispersion agent as 0.2～4% of thick pearl protein, then grind 60～600 minutes with nano-level grinder, during this time with supersonic cleaning machine concussion 20～100min, pearl protein particle is fully uniformly dispersed, is conducive to improve mill efficiency;
3) liquid, after grinding separates the liquid of 1000 molecular weight with membrane separation apparatus, obtain being greater than the solidliquid mixture of 1000 molecular weight, and this mixture is concentrated to solid-to-liquid ratio is 0.01～0.1:1, and then heat-sterilization is preserved and obtained nano-pearl albumen.
2. the preparation method of nano-pearl albumen according to claim 1, is characterized in that: described edible acid is 5～25% alimentary acetic acids.
3. the preparation method of nano-pearl albumen according to claim 1, is characterized in that: described dispersion agent is polysorbas20 or Sodium hexametaphosphate 99 liquid.
4. the preparation method of nano-pearl albumen according to claim 1, is characterized in that: described nano-level grinder refers to horizontal nanometer sand mill, and zirconium oxide balls specification used, 0.1～0.8 millimeter of particle diameter, is ground rotating speed between 200～3000 revs/min.
5. the preparation method of nano-pearl albumen according to claim 1, is characterized in that: described membrane separation apparatus refers to middle control cellulosic ultrafiltration membrane separation apparatus or Ceramic excessive filtration membrane separation apparatus.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104558137A (en) * | 2015-01-15 | 2015-04-29 | 浙江欧诗漫生物股份有限公司 | Preparation method of conchiolin as well as water-soluble conchiolin and acid-soluble conchiolin prepared by virtue of preparation method |
| CN106366156A (en) * | 2016-08-27 | 2017-02-01 | 广东澳珍药业有限公司 | Method of supercritical CO2 extraction of pearl protein fluid |
| CN107259627A (en) * | 2017-06-22 | 2017-10-20 | 黄斌华 | Integration of drinking and medicinal herbs nano micromolecule and its preparation technology and integration of drinking and medicinal herbs food |
| CN105343870B (en) * | 2015-11-30 | 2018-10-26 | 江苏邦泽生物医药技术股份有限公司 | A kind of keratin nanoparticle and preparation method thereof |
| CN111363003A (en) * | 2020-04-02 | 2020-07-03 | 浙江长生鸟健康科技股份有限公司 | By using CO2Method for extracting pearl protein by supercritical fluid technology |
| CN111777660A (en) * | 2020-07-16 | 2020-10-16 | 德兰梅勒（北京）分离技术股份有限公司 | Efficient membrane separation process for pearl powder |
| CN111850073A (en) * | 2019-04-29 | 2020-10-30 | 欧诗漫生物股份有限公司 | Pearl oyster whitening factor, preparation method and application thereof |
| TWI767343B (en) * | 2020-09-28 | 2022-06-11 | 歐詩漫生物股份有限公司 | Pearl oyster whitening factor, its preparation method and use |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104558137A (en) * | 2015-01-15 | 2015-04-29 | 浙江欧诗漫生物股份有限公司 | Preparation method of conchiolin as well as water-soluble conchiolin and acid-soluble conchiolin prepared by virtue of preparation method |
| WO2016112722A1 (en) * | 2015-01-15 | 2016-07-21 | 欧诗漫生物股份有限公司 | Preparation method for conchiolin, and water-soluble conchiolin and acid-soluble conchiolin prepared by using method |
| TWI597017B (en) * | 2015-01-15 | 2017-09-01 | Osmun Biological Co Ltd | Method for preparing pearl protein and water-soluble pearl protein and acid-soluble pearl protein prepared by the method |
| CN105343870B (en) * | 2015-11-30 | 2018-10-26 | 江苏邦泽生物医药技术股份有限公司 | A kind of keratin nanoparticle and preparation method thereof |
| CN106366156A (en) * | 2016-08-27 | 2017-02-01 | 广东澳珍药业有限公司 | Method of supercritical CO2 extraction of pearl protein fluid |
| CN107259627A (en) * | 2017-06-22 | 2017-10-20 | 黄斌华 | Integration of drinking and medicinal herbs nano micromolecule and its preparation technology and integration of drinking and medicinal herbs food |
| CN111850073A (en) * | 2019-04-29 | 2020-10-30 | 欧诗漫生物股份有限公司 | Pearl oyster whitening factor, preparation method and application thereof |
| WO2020221145A1 (en) * | 2019-04-29 | 2020-11-05 | 欧诗漫生物股份有限公司 | Pearl shell whitening factor and preparation method therefor and use thereof |
| CN111850073B (en) * | 2019-04-29 | 2024-02-13 | 欧诗漫生物股份有限公司 | Pearl Bei Meibai factor, preparation method and application thereof |
| CN111363003A (en) * | 2020-04-02 | 2020-07-03 | 浙江长生鸟健康科技股份有限公司 | By using CO2Method for extracting pearl protein by supercritical fluid technology |
| CN111777660A (en) * | 2020-07-16 | 2020-10-16 | 德兰梅勒（北京）分离技术股份有限公司 | Efficient membrane separation process for pearl powder |
| TWI767343B (en) * | 2020-09-28 | 2022-06-11 | 歐詩漫生物股份有限公司 | Pearl oyster whitening factor, its preparation method and use |
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Application publication date: 20140521 |