CN106366156A - Method of supercritical CO2 extraction of pearl protein fluid - Google Patents
Method of supercritical CO2 extraction of pearl protein fluid Download PDFInfo
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- CN106366156A CN106366156A CN201610743657.6A CN201610743657A CN106366156A CN 106366156 A CN106366156 A CN 106366156A CN 201610743657 A CN201610743657 A CN 201610743657A CN 106366156 A CN106366156 A CN 106366156A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/618—Molluscs, e.g. fresh-water molluscs, oysters, clams, squids, octopus, cuttlefish, snails or slugs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/01—Hydrolysed proteins; Derivatives thereof
- A61K38/012—Hydrolysed proteins; Derivatives thereof from animals
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Veterinary Medicine (AREA)
- Zoology (AREA)
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- Animal Behavior & Ethology (AREA)
- Molecular Biology (AREA)
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- Pharmacology & Pharmacy (AREA)
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- Bioinformatics & Cheminformatics (AREA)
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- Marine Sciences & Fisheries (AREA)
- Extraction Or Liquid Replacement (AREA)
Abstract
The invention provides a method of supercritical CO2 extraction of pearl protein fluid. The method comprises the following steps that 1, a certain quantity of pearl raw materials are weighed and ground into 40-90-mesh pearl powder; 2, the ground pearl powder in the first step is added into an ultrasonic water bath pot containing deionized water to be dispersed; 3, a HAc/NaAc buffer solution with the PH of 3.0 is added into the ultrasonic water bath pot in the second step to be hydrolyzed; 4, hydrolysate obtained in the third step is extracted through a supercritical CO2 extraction method; 5 obtained extracted protein is subjected to chemical component analysis and determination. According to the method of supercritical CO2 extraction of the pearl protein fluid, by improving a traditional pearl extraction technology, the extraction rate of pearl protein is effectively increased, the raw materials are saved, and meanwhile a reliable guarantee is provided for safety, effectiveness and quality of clinical medication of pearl medicines; the pearl protein fluid has the great use value and economic value.
Description
Technical field
The present invention relates to pearl protein abstraction technique field is and in particular to a kind of supercritical co2 extracts pearl protein liquid
Method.
Background technology
Margarita contains abundant nutritional labeling and all kinds of life source material, specifically includes: 1 amino acidses: mussel pearl angle
Protein can detect that several amino acids through hydrolysis, including essential amino acid, that is, serine, a word used in person's names propylhomoserin, leucine,
Scalable human endocrine, enhancing immunity and the defying age such as Phenylalanine, lysine etc., wherein serine, a word used in person's names propylhomoserin, are precious
Pearl plays the important substance basis of given efficacy；2 trace element: trace element includes copper, ferrum, zinc, magnesium etc., the height of their contents
Low often relevant with the color and luster of Margarita, some trace elements have health care simultaneously；3 calcium classes: calcium salt is the main composition of Margarita
Part；4 taurines: for nonprotein amino acid, human metabolism can be strengthened, and have tranquillizing and allaying excitement etc. to act on；5 small molecules are lived
Property peptide: the middle type product producing during for hydrolyzed pearl protein.Has good health-care effect；6 porphyrins and metalloporphyrin: tool is anti-
Old and feeble effect；7 vitamin b races: rich content, effect are notable, Margarita is as the character of medicine, identification, usage and consumption etc. all
Made the regulation of science, and the function of clear and definite Margarita with cure mainly for " arresting convulsion of calming the nerves, tomorrow removing nebula, removing toxic substances and promoting granulation, for palpitation with fear lose
Sleep, infantile convulsion epilepsy, mesh gives birth to nebula, and skin infection does not hold back power ", clinic also demonstrates that it to cardiovascular and cerebrovascular disease, hypertension, diabetes, mistake
Dormancy, constipation, osteoporosises, hyperosteogeny, old lumbago and skelalgia etc. are evident in efficacy, are also used to nurse one's health alcoholic liver etc..Margarita removes and contains
Chitosan-alginate microcapsules, water soluble protein, peptides, free amino acid, b vitamin, porphyrin, trace element is also contained outside Calcium Carbonate
Etc. function, however, at present in terms of the utilization of Margarita, the extraction of especially protein liquid is convenient, and the extraction ratio of pearl protein liquid is not
Height, seriously hinders the utilization to Margarita, and wastes the raw material of preciousness, need further to be improved.
Content of the invention
The technical problem to be solved is to provide one kind to be easy to operation, extraction for above-mentioned the deficiencies in the prior art
Take the method that the high supercritical co2 of rate extracts pearl protein liquid.
The present invention is that the adopted technical scheme that solves the above problems is:
The present invention provides a kind of supercritical co2The method of extraction pearl protein liquid, comprises the following steps:
(1) weigh a certain amount of Margarita raw material to be ground, described Margarita raw mill is become the Margarita powder of 40～90 mesh；
(2) ground Margarita powder in step (1) is added in the ultrasonic water bath pot fill deionized water and is disperseed；
(3) the hac/naac buffer that ph is 3.0 is added in the ultrasonic water bath pot of step (2), is hydrolyzed；
(4) hydrolyzed solution obtaining in step (3) is utilized supercritical co2Extracting process is extracted；
(5) chemical composition analysis and mensure are carried out to the extracting protein of gained.
Further, 20～80 DEG C of water-bath in step (2).
Further, the time that is hydrolyzed in step (2) is 10～300min；
Further, the hydrolyzed solution in step (4) utilizes supercritical co2Extracting process comprises the following steps that step:
(4～1) weigh 50g hydrolyzed solution and are added in extractor；
(4～2) are respectively heated to extraction kettle, separating still, basin or cool down；
(4～3) open co2Steel cylinder, makes co2After gas enters ice chest liquefaction, preheater is pumped into by high pressure frequency modulation plunger pre-
Heat, purified enter back into extraction kettle, boost to predetermined set value co2Become supercritical fluid, the protein in hydrolyzed solution is carried out
Extraction；
(4～4) extraction terminates rear accurate weighing hydrolyzed solution residue granulated slag, and calculates the extraction yield of hydrolyzed solution.
Further, co in described step (43)2Separated kettle decompression is separated Posterior circle and uses with extract.
Further, hydrolyzed solution utilizes supercritical co2In extracting process, hydrolyzed solution extracting pressure is 10～80m pa, extraction
Take 25～60 DEG C of temperature, extraction time is 0.5～10h.
The beneficial effects of the present invention is:
The present invention provides a kind of supercritical co2The method of extraction pearl protein liquid, by the extraction process to traditional Margarita
Improvement, effectively increase pearl protein extraction ratio, saved raw material, simultaneously also be Margarita medical material clinical application safely, effectively
Provide reliable guarantee with quality, there is larger use value and economic worth.
Brief description
Fig. 1 is a kind of present invention supercritical co2The supercritical co of the method for extraction pearl protein liquid2Extraction process figure；
Fig. 2 is the infrared spectrogram of embodiment 2 gained a word used in person's names propylhomoserin；
Fig. 3 is the infrared spectrogram of embodiment 2 gained threonine；
Fig. 4 is the infrared spectrogram of embodiment 2 gained serine 4；
Fig. 5 is the infrared spectrogram of embodiment 2 gained Phenylalanine 5；
Fig. 6 is the infrared spectrogram of embodiment 2 gained lysine 6.
Specific embodiment
With reference to specifically illustrating embodiments of the present invention, the mesh being only given to illustrate of these embodiments
, limitation of the invention can not be interpreted as, only for reference and explanation uses, and does not constitute to scope of patent protection of the present invention
Limit, because without departing from the spirit and scope of the present invention, the present invention can be carried out with many changes.
Embodiment 1
The present embodiment provides a kind of supercritical co2The method of extraction pearl protein liquid, comprises the following steps:
(1) weigh a certain amount of Margarita raw material to be ground, described Margarita raw mill is become the Margarita powder of 80 mesh；
(2) ground Margarita powder in step (1) is added in the ultrasonic water bath pot fill deionized water and carries out point
Dissipate；
(3) the hac/naac buffer that ph is 3.0 is added in the ultrasonic water bath pot of step (2), is hydrolyzed；
(4) hydrolyzed solution obtaining in step (3) is utilized supercritical co2Extracting process is extracted；
(5) chemical composition analysis and mensure are carried out to the extracting protein of gained.
In the present embodiment, 45 DEG C of water-bath in step (2), hydrolysis time is 35min
As shown in figure 1, the hydrolyzed solution in step (4) utilizes supercritical co2Extracting process comprises the following steps that step:
(4～1) weigh 50g hydrolyzed solution and are added in extractor；
(4～2) are respectively heated to extraction kettle, separating still, basin or cool down；
(4～3) open co2Steel cylinder, makes co2After gas enters ice chest liquefaction, preheater is pumped into by high pressure frequency modulation plunger pre-
Heat, purified enter back into extraction kettle, boost to predetermined set value co2Become supercritical fluid, the protein in hydrolyzed solution is carried out
Extraction；
(4～4) extraction terminates rear accurate weighing hydrolyzed solution residue granulated slag, and calculates the extraction yield of hydrolyzed solution.
In the present embodiment, co in described step (43)2It is 4 times that separated kettle decompression separates Posterior circle number of times with extract.
In the present embodiment, hydrolyzed solution utilizes supercritical co2In extracting process, hydrolyzed solution extracting pressure is 10m pa, extraction temperature
25 DEG C of degree, extraction time is 0.5h.
Embodiment 2
The present embodiment provides a kind of supercritical co2The method of extraction pearl protein liquid, comprises the following steps:
(1) weigh a certain amount of Margarita raw material to be ground, described Margarita raw mill is become the Margarita powder of 80 mesh；
(2) ground Margarita powder in step (1) is added in the ultrasonic water bath pot fill deionized water and is disperseed；
(3) the hac/naac buffer that ph is 3.0 is added in the ultrasonic water bath pot of step (2), is hydrolyzed；
(4) hydrolyzed solution obtaining in step (3) is utilized supercritical co2Extracting process is extracted；
(5) chemical composition analysis and mensure are carried out to the extracting protein of gained.
In the present embodiment, 45 DEG C of water-bath in step (2), hydrolysis time is 35min
Hydrolyzed solution in step (4) utilizes supercritical co2Extracting process comprises the following steps that step:
(4～1) weigh 50g hydrolyzed solution and are added in extractor；
(4～2) are respectively heated to extraction kettle, separating still, basin or cool down；
(4～3) open co2Steel cylinder, makes co2After gas enters ice chest liquefaction, preheater is pumped into by high pressure frequency modulation plunger pre-
Heat, purified enter back into extraction kettle, boost to predetermined set value co2Become supercritical fluid, the protein in hydrolyzed solution is carried out
Extraction；
(4～4) extraction terminates rear accurate weighing hydrolyzed solution residue granulated slag, and calculates the extraction yield of hydrolyzed solution.
In the present embodiment, co in described step (43)2It is 4 times that separated kettle decompression separates Posterior circle number of times with extract.
In the present embodiment, hydrolyzed solution utilizes supercritical co2In extracting process, hydrolyzed solution extracting pressure is 45mpa, extraction temperature
45 DEG C of degree, extraction time is 1h.
Embodiment 3
The present embodiment provides a kind of supercritical co2The method of extraction pearl protein liquid, comprises the following steps:
(1) weigh a certain amount of Margarita raw material to be ground, described Margarita raw mill is become the Margarita powder of 80 mesh；
(2) ground Margarita powder in step (1) is added in the ultrasonic water bath pot fill deionized water and is disperseed；
(3) the hac/naac buffer that ph is 3.0 is added in the ultrasonic water bath pot of step (2), is hydrolyzed；
(4) hydrolyzed solution obtaining in step (3) is utilized supercritical co2Extracting process is extracted；
(5) chemical composition analysis and mensure are carried out to the extracting protein of gained.
In the present embodiment, 45 DEG C of water-bath in step (2), hydrolysis time is 35min
Hydrolyzed solution in step (4) utilizes supercritical co2Extracting process comprises the following steps that step:
(4～1) weigh 50g hydrolyzed solution and are added in extractor；
(4～2) are respectively heated to extraction kettle, separating still, basin or cool down；
(4～3) open co2Steel cylinder, makes co2After gas enters ice chest liquefaction, preheater is pumped into by high pressure frequency modulation plunger pre-
Heat, purified enter back into extraction kettle, boost to predetermined set value co2Become supercritical fluid, the protein in hydrolyzed solution is carried out
Extraction；
(4～4) extraction terminates rear accurate weighing hydrolyzed solution residue granulated slag, and calculates the extraction yield of hydrolyzed solution.
In the present embodiment, co in described step (43)2It is 4 times that separated kettle decompression separates Posterior circle number of times with extract.
In the present embodiment, hydrolyzed solution utilizes supercritical co2In extracting process, hydrolyzed solution extracting pressure is 70mpa, extraction temperature
50 DEG C of degree, extraction time is 3h.
Embodiment 2 obtained experimental data is analyzed find, in the pearl protein liquid being extracted, is rich in ammonia needed by human
Base acid, i.e. a word used in person's names propylhomoserin (as shown in Figure 2), threonine (as shown in Figure 3), serine 4, Phenylalanine 5 and lysine 6.
The present invention provides a kind of supercritical co2The method of extraction pearl protein liquid, by the extraction process to traditional Margarita
Improvement, effectively increase pearl protein extraction ratio, saved raw material, simultaneously also be Margarita medical material clinical application safely, effectively
Provide reliable guarantee with quality, there is larger use value and economic worth.
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention are not subject to above-described embodiment
Limit, other any spirit without departing from the present invention and the change made under principle, modification, replacement, combine, simplify,
All should be equivalent substitute mode, be included within protection scope of the present invention.
Claims (6)
1. a kind of supercritical co2The method of extraction pearl protein liquid is it is characterised in that comprise the following steps:
(1) weigh a certain amount of Margarita raw material to be ground, described Margarita raw mill is become the Margarita powder of 40～90 mesh；
(2) ground Margarita powder in step (1) is added in the ultrasonic water bath pot fill deionized water and is disperseed；
(3) the hac/naac buffer that ph is 3.0 is added in the ultrasonic water bath pot of step (2), is hydrolyzed；
(4) hydrolyzed solution obtaining in step (3) is utilized supercritical co2Extracting process is extracted；
(5) chemical composition analysis and mensure are carried out to the extracting protein of gained.
2. a kind of supercritical co according to claim 12Extraction pearl protein liquid method it is characterised in that: step (2)
20～80 DEG C of middle water-bath.
3. a kind of supercritical co according to claim 12Extraction pearl protein liquid method it is characterised in that: step (2)
In be hydrolyzed the time be 10～300min.
4. a kind of supercritical co according to claim 12Extraction pearl protein liquid method it is characterised in that: step (4)
In hydrolyzed solution utilize supercritical co2Extracting process comprises the following steps that step:
(4～1) weigh 50g hydrolyzed solution and are added in extractor；
(4～2) are respectively heated to extraction kettle, separating still, basin or cool down；
(4～3) open co2Steel cylinder, makes co2After gas enters ice chest liquefaction, preheater preheating is pumped into by high pressure frequency modulation plunger,
Purified enter back into extraction kettle, boost to predetermined set value co2Become supercritical fluid, the protein in hydrolyzed solution is extracted
Take；
(4～4) extraction terminates rear accurate weighing hydrolyzed solution residue granulated slag, and calculates the extraction yield of hydrolyzed solution.
5. a kind of supercritical co according to claim 42Extraction pearl protein liquid method it is characterised in that: described step
Suddenly co in (43)2Separated kettle decompression is separated Posterior circle and uses with extract.
6. a kind of supercritical co according to claim 42Extraction pearl protein liquid method it is characterised in that: hydrolyzed solution profit
Use supercritical co2In extracting process, hydrolyzed solution extracting pressure is 10～80mpa, 25～60 DEG C of extraction temperature, and extraction time is 0.5
～10h.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610743657.6A CN106366156A (en) | 2016-08-27 | 2016-08-27 | Method of supercritical CO2 extraction of pearl protein fluid |
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| CN201610743657.6A CN106366156A (en) | 2016-08-27 | 2016-08-27 | Method of supercritical CO2 extraction of pearl protein fluid |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111363003A (en) * | 2020-04-02 | 2020-07-03 | 浙江长生鸟健康科技股份有限公司 | By using CO2Method for extracting pearl protein by supercritical fluid technology |
| CN114080229A (en) * | 2019-05-13 | 2022-02-22 | Mbp(毛里求斯)有限公司 | Method for separating molecules contained in organic mineral layers of marine bivalve mollusk shells |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114080229A (en) * | 2019-05-13 | 2022-02-22 | Mbp(毛里求斯)有限公司 | Method for separating molecules contained in organic mineral layers of marine bivalve mollusk shells |
| CN111363003A (en) * | 2020-04-02 | 2020-07-03 | 浙江长生鸟健康科技股份有限公司 | By using CO2Method for extracting pearl protein by supercritical fluid technology |
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