CN101167753A - Application of sea collagen peptide as preparation of hypoglycemic medicine, health-care food or food - Google Patents
Application of sea collagen peptide as preparation of hypoglycemic medicine, health-care food or food Download PDFInfo
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- CN101167753A CN101167753A CNA2007101576418A CN200710157641A CN101167753A CN 101167753 A CN101167753 A CN 101167753A CN A2007101576418 A CNA2007101576418 A CN A2007101576418A CN 200710157641 A CN200710157641 A CN 200710157641A CN 101167753 A CN101167753 A CN 101167753A
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Abstract
The invention discloses a use of ocean collagen peptide used in preparation of blood-glucose medicine, hypoglycemic health food or hypoglycemic food, which is characterized by high absorption, fast absorption, low viscosity, and good water-solubility etc. Experiment proves that the ocean collagen peptide given to a little mouse can lower alloxan hyperglycosemia of fasting blood-glucose level of a big mouse. Meanwhile, sugar tolerance of a hyperglycemia big mouse is improved, which indicates that the ocean collagen peptide has hypoglycemic action.
Description
Technical field:
The present invention relates to the new purposes of marine collagen, is the purposes of marine collagen as preparation hypoglycemic drug, hypoglycemia healthcare food or blood sugar lowering food (containing health food) specifically.Belong to health care of food and field of medicaments.
Background technology:
In recent years, along with the aggravation of environmental pollution and the prolongation of human longevity, cardiovascular and cerebrovascular disease, malignant tumor, diabetes, osteoporosis diseases are day by day seriously threatening human health.Diabetes are metabolic diseases of a kind of multi-pathogenesis, and prevalence increases day by day, have become disaster and challenge that the mankind face, are one of worldwide public health problems.For treatment of diabetes is based on blood sugar control, and some hypoglycemic drugs that adopt all have side effect in various degree at present, and life-time service can produce harm to health.Therefore, the mankind press for new, the effective and safe method of searching and prevent and treat diabetes.WHO diet control in the diabetes " modern composite treatment (five drive carriage) " that the whole world proposes makes number one, and the health food that therefore has the blood sugar regulation effect becomes the research focus in diabetes control field.
Tellurian marine resources is abundant, marine organism contains the rich in protein composition, by the bioactive peptide that biological enzymolysis technology extracts from marine organisms, nutritive value is very high, has special physiological functions such as antibiotic, antioxidation, blood pressure lowering, antitumor, adjusting immunity respectively.Contain abundant collagenic protein, aminoacid etc. in the fish skin, but went out of use usually as the leftover bits and pieces of aquatic products processing in the past, cause the wasting of resources to influence environment again.At present, (company standard number is Q/HSBS1-2006 to the marine collagen that Zhejiang Hai Shi bio tech ltd is produced, the number of putting on record of Zhoushan Zhejiang Bureau of Technical Supervision is Q330900.X20.1121-2006, hygiene permits is No. the 33090310003rd, Zhe Wei food card word 2006) be exactly with the marine fishes (Oncorhynchi widely of originating, anglerfish, salmon fish, salmon, tilapia etc.) fish skin is a raw material, produce molecular weight at 200～1000 oligo peptide by hydrolysis, belong to biologically active peptide, has easy absorption, absorb fast, low viscosity, characteristics such as good water solubility, a proteinic as a supplement nutrient additive for food at present.Up to now, any report of also in blood sugar lowering medicine, hypoglycemia healthcare food and blood sugar lowering food, not using about marine collagen.
Summary of the invention:
The present invention is in order to solve the problem of the existing existing side effect of blood sugar control medicine, a kind of easy absorption is provided, absorbs fast, low viscosity, the marine collagen of good water solubility is in the purposes of preparation hypoglycemic drug, hypoglycemia healthcare food or blood sugar lowering food.
Technical solution of the present invention is: the purposes of marine collagen in preparation hypoglycemic drug, hypoglycemia healthcare food or blood sugar lowering food.
Described medicine and health food are powder, granule, capsule, tablet, pill or oral liquid.
Described food is starch-based product, milk and milk products, bean product or baking goods.
The present invention has found that existing material-marine collagen has the hypoglycemic function of prevention, invented the purposes of marine collagen, had easy absorption, absorb fast, low viscosity, characteristics such as good water solubility as preparation hypoglycemic drug, hypoglycemia healthcare food or blood sugar lowering food.Experiment confirm gives the fasting blood glucose level that the mice marine collagen can obviously reduce the alloxan hyperglycemic rat; Can improve simultaneously the carbohydrate tolerance of hyperglycemic rat, show that marine collagen has hypoglycemic activity.
The specific embodiment:
Embodiment:
(company standard number is Q/HSBS1-2006 to the marine collagen of producing with Zhejiang Hai Shi bio tech ltd, the number of putting on record of Zhoushan Zhejiang Bureau of Technical Supervision is Q330900.X20.1121-2006) be raw material, can in preparation hypoglycemic drug or blood sugar lowering food, use.Marine collagen and other are medicinal or edible adjuvant is made hypoglycemic drug or hypoglycemia healthcare food can be powder, granule, capsule, tablet, pill, oral liquid etc.; Blood sugar lowering food is that marine collagen is added on starch-based product, milk and milk products, bean product, baking goods is medium.
The radiation-resistant animal experiment study of preparation of the present invention is as follows:
1. material and method
1.1 sample: marine collagen (the MarineCollagen Peptides that Beijing crapulous syncope Hai Shi Bioisystech Co., Ltd produces, MCPs), fish skin with deep-sea salmon fish, salmon, tilapia etc. is a raw material, through cleaning homogenate → compound coupling enzymolysis → enzyme deactivation → defat → multistage membrance separation → infiltration refining → concentrated → technologies such as decolouring → granulating and drying make, company standard number is Zhoushan, Zhejiang Q330900.X20.1121-2006;
1.2 laboratory animal: cleaning level Sprague-Dawley (SD) rat, male, body weight 160～250g, provided by Department Of Medicine, Peking University's Experimental Animal Center, approval number is SCXK11-00-0004, and cleaning level Animal House is raised, 21～23 ℃ of feeding environment temperature, humidity 50～60%RH.
1.3 key instrument and reagent
1.3.1 reagent: alloxan (U.S. Sigma company); Blood glucose (Glu) is measured test kit (Zhongsheng Beikong Biological Science ﹠ Technology Co., Ltd.); Other reagent are analytical pure.
1.3.2 instrument: HITACHI7170 automatic clinical chemistry analyzer (Japan).
1.4 experimental technique
After the animal fasting 24 hours, lumbar injection alloxan (170mg/kg) is irritated stomach and is given 50% glucose filling stomach after 3～5 hours.After 7 days, fasting 3-5 hour, to get tail vein and measure blood glucose, blood glucose value is a hyperglycemia model success animal greater than 10mmol/L person.
Select the hyperglycemia model animal to be divided into 4 groups at random, comprise basic, normal, high 3 the dosage groups of hyperglycemia model matched group and MCPs by blood sugar level.The MCPs group is irritated stomach and is given variable concentrations MCPs, is respectively 0.225g/kg.bw, 0.45g/kg.bw and 1.35g/kg.bw, is equivalent to 5 times, 10 times and 30 times of human body recommended dose (2.7g/60kg.bw), and the hyperglycemia model matched group is irritated stomach and given the equal-volume solvent.The alternative normal rat is divided into 2 groups at random by fasting blood glucose level, comprises normal control group (irritating stomach equal-volume solvent, 12) and high dose matched group (irritating stomach equal-volume high dose MCPs peptide, 13).More than 6 treated animal ad libs (normal diet), the water inlet, raised for 8 weeks continuously.
1.5 detection index:
1.5.1 fasting glucose is measured: each is organized rat and gets tail vein on an empty stomach at the 0th week, 2 weekends, 4 weekends, 6 weekends, 8 weekends respectively, and separation of serum is measured fasting blood glucose level.
1.5.2 carbohydrate tolerance experiment: irritate stomach after 60 days, hyperglycemia model or the fasting of insulin resistant model animal are after 4 hours, the MCPs group gives variable concentrations MCPs, model control group gives to irritate stomach with the volume solvent, per os gives glucose 2.0g/kg.bw after 15～20 minutes, measure to give behind the glucose 0,0.5,2 hour blood glucose value, calculate area under the blood glucose curve.
1.6 statistical method:
Numerical value is represented with mean ± standard deviation, uses the SPSS statistical analysis software, adopts the difference of the more every index of one factor analysis of variance between experimental group and matched group.
2. result
2.1 MCPs is to the influence of the inductive hyperglycemia model rat fasting blood-glucose of alloxan
Before the high sugared model control group rat experiment and the fasting blood glucose level after the experiment all apparently higher than the normal control group, show that high sugared model sets up; The administration of MCPs high dose group rat during 2 weekends fasting blood glucose level be starkly lower than high sugared model control group (P＜0.05); During 8 weekends, the basic, normal, high dosage group of MCPs fasting blood glucose level all is starkly lower than high sugared model control group (P＜0.05); Fasting blood glucose level all is starkly lower than high sugared model control group before and after the normal MCPs high dose control rats experiment, compares no significant difference (table 1) after the experiment with before the experiment.The above results shows that MCPs has the effect that reduces the diabetes rat fasting blood glucose level; But the fasting blood glucose level to normal rat does not have obvious influence.
Table 1.MCPs is to the influence of the inductive hyperglycemic rat fasting blood glucose level of alloxan
| Group | Dosage (g/kg.bw) | Number of animals (only) | Fasting blood sugar (mmol/L) | ||||
| 0w | 2w | 4w | 6w | 8w | |||
| The normal high dose matched group of the high sugared model control group normal control group of dosage group high dose group in the low dose group | 0.225 0.450 1.350 0 0 1.350 | 12 8 11 11 12 13 | 27.73±4.52 25.71±4.11 25.84±5.59 26.43±7.24 5.66±0.38 * 5.66±0.43 * | 38.53±1.63 38.80±0.95 35.78±10.01 * 40.20±3.92 6.28±0.78 * 5.65±1.01 * | 41.19±3.20 40.60±1.13 39.75±1.32 39.73±3.31 5.90±0.66 * 6.20±0.30 * | 47.63±3.86 43.45±5.28 * 43.80±2.74 * 48.05±2.05 6.05±0.38 * 5.92±0.41 * | 42.71±7.86 * 39.06±6.66 * 41.32±13.02 * 51.12±10.94 8.08±0.70 * 8.28±0.54 * |
*Difference is compared in expression with the sugared model control group of height have significance (P＜0.05)
2.2 MCPs is to the influence of the inductive hyperglycemia model rat of alloxan carbohydrate tolerance
The carbohydrate tolerance experimental result sees Table 2, in the MCPs high dose group rat carbohydrate tolerance experiment under 0h blood sugar level and the blood glucose curve area be starkly lower than high sugared model control group (P＜0.05), do not have notable difference between normal high dose group and the normal control group.The above results shows that MCPs has some improvement for the carbohydrate tolerance tool of hyperglycemic rat.
Table 2.MCPs induces the influence of inductive hyperglycemic rat carbohydrate tolerance to alloxan
| Group | Dosage (g/kg.bw) | Number of animals (only) | Blood glucose value (mmol/L) | Area under the blood glucose curve | ||
| 0h | 0.5h | 2h | (mmol/L) | |||
| The normal high dose matched group of the high sugared model control group normal control group of dosage group high dose group in the low dose group | 0.225 0.450 1.350 0 0 1.350 | 12 8 11 11 12 13 | 48.49±7.66 45.76±7.65 42.61±10.22 * 47.63±10.18 4.82±0.64 * 5.12±0.49 * | 57.44±7.71 53.09±10.56 50.02±9.07 54.21±6.91 5.96±0.56 * 6.20±0.86 * | 44.44±7.27 49.48±9.89 43.69±11.88 46.16±6.20 6.88±0.61 * 7.97±0.50 * | 102.89±13.69 101.64±17.47 90.34±16.97 * 100.74±13.05 12.32±1.03 * 13.46±1.17 * |
*Difference is compared in expression with the sugared model control group of height have significance (P＜0.05)
3. conclusion
Alloxan hyperglycemic rat per os gives marine collagen after 8 weeks, and its testing result shows that marine collagen has tangible reduction effect to the fasting blood glucose level of alloxan hyperglycemia model rat; Carbohydrate tolerance tool to the hyperglycemia model rat has some improvement simultaneously; To sum up the result shows that marine collagen has the blood sugar lowering effect, can use in preparation hypoglycemic drug, hypoglycemia healthcare food or blood sugar lowering food.
Claims (3)
1. marine collagen is as the purposes of preparation hypoglycemic drug, hypoglycemia healthcare food or blood sugar lowering food.
2. marine collagen according to claim 1 is as the purposes of preparation hypoglycemic drug, hypoglycemia healthcare food or blood sugar lowering food, and it is characterized in that: described medicine, health food are powder, granule, capsule, tablet, pill or oral liquid.
3. marine collagen according to claim 1 is as the purposes of preparation hypoglycemic drug, hypoglycemia healthcare food or blood sugar lowering food, and it is characterized in that: described food is starch-based product, milk and milk products, bean product or baking goods.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2007101576418A CN101167753A (en) | 2007-10-24 | 2007-10-24 | Application of sea collagen peptide as preparation of hypoglycemic medicine, health-care food or food |
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| CNA2007101576418A CN101167753A (en) | 2007-10-24 | 2007-10-24 | Application of sea collagen peptide as preparation of hypoglycemic medicine, health-care food or food |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102077934A (en) * | 2010-09-20 | 2011-06-01 | 王强 | Special dietary marine food for patients with high blood pressure |
| CN102077936A (en) * | 2010-09-20 | 2011-06-01 | 王强 | Special sea dietary food for diabetics |
| CN109362940A (en) * | 2018-10-12 | 2019-02-22 | 常同喜 | A kind of preparation method of marine collagen peptide and antihypelipidemic preparation |
| CN112569347A (en) * | 2020-12-30 | 2021-03-30 | 青海瑞肽生物科技有限公司 | Application of yak collagen peptide in hypoglycemic drugs or hypoglycemic health foods |
-
2007
- 2007-10-24 CN CNA2007101576418A patent/CN101167753A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102077934A (en) * | 2010-09-20 | 2011-06-01 | 王强 | Special dietary marine food for patients with high blood pressure |
| CN102077936A (en) * | 2010-09-20 | 2011-06-01 | 王强 | Special sea dietary food for diabetics |
| CN102077934B (en) * | 2010-09-20 | 2013-02-27 | 王强 | Special dietary marine food for patients with high blood pressure |
| CN102077936B (en) * | 2010-09-20 | 2013-04-24 | 王强 | Special sea dietary food for diabetics |
| CN109362940A (en) * | 2018-10-12 | 2019-02-22 | 常同喜 | A kind of preparation method of marine collagen peptide and antihypelipidemic preparation |
| CN112569347A (en) * | 2020-12-30 | 2021-03-30 | 青海瑞肽生物科技有限公司 | Application of yak collagen peptide in hypoglycemic drugs or hypoglycemic health foods |
| CN112569347B (en) * | 2020-12-30 | 2023-09-22 | 青海瑞肽生物科技有限公司 | Application of yak collagen peptide in hypoglycemic drugs or hypoglycemic health-care foods |
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Open date: 20080430 |