CN101999671A - Application of silkworm pupa protein - Google Patents
Application of silkworm pupa protein Download PDFInfo
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- CN101999671A CN101999671A CN2010105332556A CN201010533255A CN101999671A CN 101999671 A CN101999671 A CN 101999671A CN 2010105332556 A CN2010105332556 A CN 2010105332556A CN 201010533255 A CN201010533255 A CN 201010533255A CN 101999671 A CN101999671 A CN 101999671A
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- pupa albumen
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Abstract
The invention discloses application of silkworm pupa protein in fatigue resistance under an anaerobic environment. The silkworm pupa protein is used for delaying the generation of fatigue and accelerating fatigue elimination. The silkworm pupa protein has the effect of improving the exercise tolerance, delaying the generation of the fatigue and accelerating the fatigue elimination under the anaerobic environment, and the silkworm pupa protein has certain application prospect on the aspect of improving plateau working capacity.
Description
Technical field
The present invention relates to biological technical field, be specifically related to pupa albumen application in the antifatigue under anaerobic environment.
Background technology
Silkworm chrysalis (Silkworm pupa) is the pupa of Bombycidae insect bombyx mori, is a kind of cuisines of high nutrition, also is a kind of Chinese medicine, has the effect with taste, wet, the long yang-energy that dispels the wind, and just obtains extensive use since ancient times.Modern medicine proves that silkworm chrysalis has effects such as the cholesterolemia of reduction, treatment coronary insufficiency.
Edible silkworm protein powder is 21 century novel nourishing source, and the pupa albumen powder of producing behind deodorization decoloring has kept the beneficiating ingredient of former silkworm chrysalis, and it is smelly to have removed unpleasant pupa.Do not add any anticorrisive agent, have natural prescription, comprehensive nutrition, equilibrium and replenish, be easy to distinguishing features such as absorption.Edible silkworm protein powder is high activity, low-fat full-valence animal protein, 9 kinds of essential amino acids that amino acid A wide selection of colours and designs, especially human body can not synthesize voluntarily, vitamin, mineral matter, micro-equal size abundance, ratio is suitable, is fit to very much the human body needs.
Edible silkworm protein powder is a high-quality natural animal albumen, have promote to grow, build up health, diseases prevention and the anti-ageing multiple function of waiting for a long time, can be widely used in functional health-care food, the personnel that are fit to children, old man, pregnant woman, valetudinarian, sportsman and other extraordinary work down are edible.
The concrete effect of pupa albumen is as follows:
One, trophism.Research thinks that silkworm compound amino acid can promote the growth of children rat in age and keep the function of nitrogen metabolism.Pupa albumen is identical with casein to the effect that promotes animal growth.Pupa albumen is a kind of good protein, and every nutrition index is consistent with casein.The pupa albumen powder can also add biscuit to and make silkworm chrysalis child high protein biscuit, to promoting that upgrowth and development of children has good result.
Two, improve immunologic function.Compound amino acid is extracted in the silkworm chrysalis hydrolysis, confirm that through zoopery silkworm compound amino acid can obviously improve immunocompromised mouse T and drench commentaries on classics and B lymphocyte transformation function.And can improve the normal mouse cold resistance, anti-anoxic, pole-climbing ability and promotion hematopoiesis function.The silkworm chrysalis polysaccharide has the effect of obvious enhancing body's immunity.
Three, liver protection effect.Studies confirm that pupa albumen has the effect of the experimental hepatitis rat blood serum glutamic-pyruvic transaminase of reduction.Go up marine mountain hospital report, add tangerine peel with the degreasing silkworm chrysalis and make the compound silkworm chrysalis powder and be used for the treatment of chronic liver disease, cirrhosis and advanced schistosomiasis and obtain certain effect.It is effective in cure with the treatment hypercholesterolemia to improving liver function to make pill with the silkworm pupal fat in addition.
Four, antitumor action.Studies confirm that silkworm compound amino acid has the obvious suppression effect to the knurl bulk-growth of S-180 tumor-bearing mice, and can promote mice with tumor NK cytoactive.With the silkworm chrysalis is that the silkworm chrysalis Cordyceps sinensis that matrix is cultivated has obvious inhibitory action to mouse S-180.Silkworm chrysalis Cordyceps sinensis all has the obvious treatment effect to cancer of the stomach, liver cancer.
Five, other.It is reported that the silkworm chrysalis water extract has obvious hypoglycemic activity to the hyperglycaemia mouse.External report Pupa bombycis extract can be promoted the absorption of skin to cosmetics, is used for veterinary drug and can strengthens antitoxic action.
But, the present report that does not also have pupa albumen application in the antifatigue under anaerobic environment.
Summary of the invention
In order to address the above problem, the invention discloses pupa albumen to the application in the antifatigue under the anaerobic environment.Wherein pupa albumen is used for the delay fatigue generation, and can be used to accelerate elimination of fatigue.Described pupa albumen is used to improve exercise tolerance.Above-mentioned pupa albumen can be applicable to altitude environment.
The invention has the beneficial effects as follows pupa albumen to having delay fatigue under the anaerobic environment and produce and accelerate elimination of fatigue, and have certain application prospect aspect the labour capacity of plateau improving.
Introduced the notion of a series of reduced forms in the summary of the invention part, this will further describe in specific embodiment part.Content part of the present invention does not also mean that key feature and the essential features that will attempt to limit technical scheme required for protection, does not more mean that the protection domain of attempting to determine technical scheme required for protection.
Description of drawings
Do not have.
The specific embodiment
In the following description, a large amount of concrete details have been provided so that more thorough understanding of the invention is provided.Yet, it will be apparent to one skilled in the art that the present invention can need not one or more these details and implemented.In other example,, be not described for technical characterictics more well known in the art for fear of obscuring with the present invention.
In order thoroughly to understand the present invention, detailed structure will be proposed in following description.Obviously, execution of the present invention is not limited to the specific details that those skilled in the art has the knack of.Preferred embodiment of the present invention is described in detail as follows, yet except these were described in detail, the present invention can also have other embodiments.
The following examples explanation pupa albumen is to having the effect that delay fatigue produces and accelerates elimination of fatigue under the anaerobic environment.
The embodiment explanation:
100 of male mice in kunming (n=20), be divided into 5 groups at random by body weight, be respectively dosage group (2.5g/kgd) and pupa albumen high dose group (5g/kgd) in normal oxygen control group, anoxic control group, pupa albumen low dose group (0.5g/kgd), the pupa albumen.Grouping and method of disposal see Table 1.
Table 1 grouping and method of disposal
| Grouping | The example number | Method of disposal |
| Normal oxygen control group | 20 | Under normal oxygen environment, raise |
| The anoxic control group | 20 | In simulation 5000m altitude environment, raise |
| Low dose group | 20 | In simulation 5000m altitude environment, raise |
| Middle dosage group | 20 | In simulation 5000m altitude environment, raise |
| High dose group | 20 | In simulation 5000m altitude environment, raise |
1, pupa albumen is to the influence of mouse physique amount
Difference (P＞0.05) that the physique amount of testing the 1st day, 5 days, 10 days mouse compares with control group that there are no significant.
2, pupa albumen exhausts the influence of swimming time to mouse power
The last administration after 1 hour every group get 10 mouse at random and begin swimming test, normal oxygen control group is finished detection at the Plain environment, anoxic control group and pupa albumen group are finished detection in hypobaric chamber.Partial pressure of oxygen is equivalent to height above sea level 5000m height in the hypobaric chamber.Mouse is placed the swimming case, depth of water 50cm, water temperature (25 ± 0.5) ℃ applies current by water pump and disturbs, and reaches the effect that applies the swimming load to mouse.Record from swimming begin to time that the whole submergeds of head can not emerge in 10 seconds as the mouse swimming time.The result shows, with the anoxic control group relatively, pupa albumen low dose group and middle dosage group significant prolongation mouse power exhaust swimming time (P＜0.01), be respectively 2.61 times and 5.18 times of control group.And high dose group mouse swimming time does not have marked change.Plain control group and anoxic control group mice swimming time do not have significant difference, see Table 2.
Swimming test is the index of reflection animal movement endurance, and the raising of exercise tolerance is that anti-fatigue ability is strengthened the strongest macro manifestations.This experiment shows that pupa albumen significant prolongation anoxic mouse power exhausts swimming time, shows that pupa albumen has the effect that improves mouse movement endurance under the anaerobic environment.
3, pupa albumen is to the influence of Mouse Liver glycogen content
The last administration after 1 hour every group get 10 mouse sacrificed by decapitation, take by weighing the 50-100mg liver and after the physiological saline rinsing, blot with filter paper, carry out hepatic glycogen content by the kit specification and measure.The result shows that anoxic control group mice hepatic glycogen content is significantly higher than normal oxygen control group (P＜0.05), and pupa albumen low dose group and middle dosage group hepatic glycogen content are significantly higher than anoxic control group (P＜0.01), have raise 55% and 37% respectively.And high dose group and anoxic control group are relatively, and the Mouse Liver glycogen content does not obviously change, and sees Table 2.And hepatic glycogen can resolve into glucose and enters blood, keeps blood sugar concentration, and hepatic glycogen deposit and exercise tolerance are proportionate, and is the index of estimating body resisting fatigue ability.This shows that pupa albumen increases hepatic glycogen content, and the prompting pupa albumen has the effect that increases anoxic mouse movement endurance.
4, pupa albumen to mouse movement after the influence eliminated of blood lactic acid
Be used for the mouse that power exhausts swimming test, respectively at swimming is preceding, swimming power exhausts at once, power exhausts 20 minutes three time points of back rest and adopts tail point blood, measure the blood lactate level with blood lactic acid test paper, calculate blood lactic acid TG-AUC, judge the influence of pupa albumen blood lactic acid elimination of level.Blood lactic acid TG-AUC=5 * (blood lactic acid value before the swimming+3 * swimming power exhausts at once, and blood lactic acid value+2 * power exhausts 20 minutes blood lactic acid values of back rest).The result shows, compares with the anoxic control group, and middle dosage group blood lactic acid TG-AUC significantly reduces, and has reduced by 49.72% (P＜0.01), and other group does not relatively have significant difference with the anoxic control group, sees Table 2.
Blood lactic acid is the main metabolites of muscle activity, and lactic acid can make myocyte's pH value descend, and then causes a series of biochemical changes, and blood lactic acid TG-AUC can reflect lactic acid elimination process, is used for judging whether tried thing has the effect that accelerates elimination of fatigue.This experimental result shows that pupa albumen reduces anoxic mouse blood lactic acid TG-AUC, and the prompting pupa albumen has the effect that the mouse that moves is accelerated elimination of fatigue under altitude environment.
Table 2 pupa albumen is to the antifatigue effect of anoxic mouse
5, pupa albumen is to the influence of mouse blood sugar level
Be used for the mouse that power exhausts swimming test,, measure blood-sugar content with blood sugar test paper respectively at swimming is preceding, swimming power exhausts at once, power exhausts back three time points of rest 20 normal oxygen and adopts tail point blood.The result shows, normal oxygen control group mice blood sugar is a little more than the anoxic group, and wherein power exhausts moment blood sugar (P＜0.01) and rest 20 normal oxygen blood sugar (P＜0.05) are significantly higher than the anoxic control group.Basic, normal, high three dosage group blood sugar are compared with the anoxic control group does not have marked change, sees Table 3.
Table 3 pupa albumen to the influence of mouse blood sugar level (mmol/L,
)
Compare with the anoxic control group: * P＜0.05, * * P＜0.01
Studies show that at present pupa albumen has the antifatigue effect under normal oxygen condition, but whether it has antifatigue effect and still do not have relevant laboratory data under anoxia condition.This research is observed, pupa albumen has the effect that prolongation anoxic mouse power exhausts swimming time, increase hepatic glycogen content, reduces blood lactic acid TG-AUC, the prompting pupa albumen has improve the effect that exercise tolerance, delay fatigue produce and accelerate elimination of fatigue under altitude environment, shows that pupa albumen has certain application prospect aspect the labour capacity of plateau improving.
The present invention is illustrated by the foregoing description, but should be understood that, the foregoing description just is used for for example and illustrative purposes, but not is intended to the present invention is limited in the described scope of embodiments.It will be appreciated by persons skilled in the art that in addition the present invention is not limited to the foregoing description, can also make more kinds of variants and modifications according to instruction of the present invention, these variants and modifications all drop in the present invention's scope required for protection.Protection scope of the present invention is defined by the appended claims and equivalent scope thereof.
Claims (5)
1. pupa albumen application aspect the antifatigue under anaerobic environment.
2. application according to claim 1 is characterized in that, described pupa albumen is used for delay fatigue and produces.
3. application according to claim 1 is characterized in that described pupa albumen is used to accelerate elimination of fatigue.
4. application according to claim 1 is characterized in that described pupa albumen is used to improve exercise tolerance.
5. according to each described application of claim 1-4, it is characterized in that described pupa albumen is applied to altitude environment.
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| CN2010105332556A CN101999671A (en) | 2010-11-05 | 2010-11-05 | Application of silkworm pupa protein |
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| CN2010105332556A CN101999671A (en) | 2010-11-05 | 2010-11-05 | Application of silkworm pupa protein |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103584117A (en) * | 2013-10-17 | 2014-02-19 | 宿州市皖神面制品有限公司 | Method for producing silkworm chrysalis protein powder |
| CN103783542A (en) * | 2014-02-20 | 2014-05-14 | 桂林理工大学 | Formula of meat balls containing silkworm pupa protein |
| CN106260449A (en) * | 2016-08-26 | 2017-01-04 | 广西汇智生产力促进中心有限公司 | A kind of pupa albumen soft sweet |
| CN106616446A (en) * | 2017-01-20 | 2017-05-10 | 西南大学 | Meat product containing silkworm pupa protein and making method of meat product |
-
2010
- 2010-11-05 CN CN2010105332556A patent/CN101999671A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 《食品科学》 20051231 张丽萍等 《蚕蛹蛋白抗疲劳作用人群试食实验》 462-465 1-5 第26卷, 第9期 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103584117A (en) * | 2013-10-17 | 2014-02-19 | 宿州市皖神面制品有限公司 | Method for producing silkworm chrysalis protein powder |
| CN103783542A (en) * | 2014-02-20 | 2014-05-14 | 桂林理工大学 | Formula of meat balls containing silkworm pupa protein |
| CN106260449A (en) * | 2016-08-26 | 2017-01-04 | 广西汇智生产力促进中心有限公司 | A kind of pupa albumen soft sweet |
| CN106616446A (en) * | 2017-01-20 | 2017-05-10 | 西南大学 | Meat product containing silkworm pupa protein and making method of meat product |
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Application publication date: 20110406 |