CN1301666C - Health food capable of raising immunity and its production process - Google Patents
Health food capable of raising immunity and its production process Download PDFInfo
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- CN1301666C CN1301666C CNB2005100815997A CN200510081599A CN1301666C CN 1301666 C CN1301666 C CN 1301666C CN B2005100815997 A CNB2005100815997 A CN B2005100815997A CN 200510081599 A CN200510081599 A CN 200510081599A CN 1301666 C CN1301666 C CN 1301666C
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Abstract
The present invention discloses a health-care food capable of enhancing the immunity and a making method thereof. The health-care food capable of enhancing the immunity is prepared from the components of 600 to 900 parts by weight of milkvetch root, 400 to 600 parts by weight of rhodiola root, 400 to 600 parts by weight of tricholoma matsutake and 100 to 200 parts by weight of American ginseng. Since the health-care food is scientifically prepared from the components, the food has the advantages of reasonable formula, effect of oxygen deficiency, cold and tumor immunity resistance on organisms because of the combined use of the components, human body non-specific and specific immunity regulation, comprehensive and systematic enhancement of organism immunity and enhancement of the immunity of low-immunity people. The present invention adopts natural products or is prepared from natural products, and therefore, the present invention is a safe natural health-care food which has no toxic or side effect and can be taken for a long time.
Description
(1) technical field:
The present invention relates to a kind of health food, particularly a kind of health food that can strengthen immunity; The invention still further relates to the preparation method of this health food.
(2) background technology:
Inside of human body not only has the complete protection immune system of a cover, as lymphoid tissue under thymus gland, spleen, lymph node, the enteron aisle respiratory mucosa etc., can produce the immunocompetent cell of carrying out immunologic function but also have, as T cell, bone-marrow-derived lymphocyte, macrophage etc., these immunocompetent cells, under nerve, body fluid and immune self adjusting, coordinate to carry out the function of oneself mutually, be in a homeostatic normal condition to guarantee human body.Modern immunology is thought; the immunologic function of human body has defence infection, homeostasis and immunosurveillance three to act on greatly; under normal circumstances; body keeps poised state; and the dependence immunologic function, resist various infection, remove harmful substance in the body; i.e. " oneself's identification ", " getting rid of the dissident ", reach homeostatic physiology protective effect.But when immunologic dysfunction, the stable state of self will be broken, and cause the various autoimmune disease, as the generation of rheumatoid arthritis, lupus erythematosus, chorionitis, Behcet, AIDS and other disease, and close ties are all arranged with the generation of the aging of human body and tumour, hypertension, diabetes etc.The immunity of modern medicine indication, also said to traditional Chinese medicine " healthy tendency " has similar implication.Theory of traditional Chinese medical science thinks that all paathogenic factors are perverse trend, and all resistances against diseases are healthy tendency.Healthy tendency can be divided into the qi of zang-fu viscera of defending gas, vigour and main histoorgan etc. again.It is to play the evil healthy tendency of invading human body outside the defence that what is called is defended gas, and it is capable outside arteries and veins to defend gas, has moistening skin, nourishes space between skin and muscles, resists outer heresy, the effect of defendance body.Defending gas and why can play a role, mainly is to rely on the adjusting of lung qi and a surname to send out.Vigour is the motive power of the activity of earning a bare living, and the traditional Chinese medical science thinks that the kidney being the origin of congenital constitution, and vigour is hidden in kidney, the kidney generating marrow and dominating bone and reproduction, and vigour can excite and promote the physiological activity of each histoorgan of whole body in the kidney, keeps growing and life of human body.The generation of the gas of five hollow organs six internal organs also depends on the subsidy of vigour.In addition, the spleen being the foundation of acquired constitution has the precise and tiny function of transporting and transforming nutrients from foodstuff, is bearing digest food, changes angry blood, fails being distributed in whole body again, moistens the important duty of supporting other internal organs.Spleen is a source of generating QI and blood thus, with body's immunological function confidential relation is arranged also.This shows that the healthy tendency of body is strong and weak dirty closely related with lung, spleen, kidney three.Human righteousness's strong and weak prosperity and decline directly determine disease generation, develop and lapse to, if healthy tendency is vigorous, body just can eliminating evil going out, avoid sick, i.e. so-called " healthy tendency internal memory, heresy can not be done ".If the positive deficiency of vital energy declines, then perverse trend is easily invaded body and is caused that disease, promptly so-called political affairs disease are because " institute of heresy beats, and its gas must be empty ".Evil positive growth and decline are also depended in advancing of disease and lapsing to, and are just winning then evil negatively, and disease is promptly to more; The evil victory and positive and negative, disease is then aggravated.Therefore the traditional Chinese medical science has proposed the basic principle of strengthening vital QI to eliminate pathogenic factors to consolidate in the process of preventing and curing diseases, and this is consistent with the health-care method that strengthens body immunity.The strengthened immunity medicament categories of being sold in the market is numerous, for example application number is: 03139711.5, name is called " a kind of antiviral agent and preparation method who improves immunity ", disclosing a kind of is the medicine of forming at 1: 3 by inosine with to the molecular proportion of acetaminobenzoic acid dimethylamino isopropyl alcohol ester, microcapsule formulations with starch embedding, it is to play antivirus action by improving body immune function, this product is stable, drug action is remarkable, and its existing antivirus action has the raising body immune function again.Therefore but it is bigger that this class can strengthen its side effect of drug products of immunity, is difficult for long-term, heavy dose of taking, and also just can not reach the effect of the enhancing immunity of lasting, system.
(3) summary of the invention:
The present invention will disclose a kind of safely, have no side effect, and health food that can comprehensive and systematic enhancing body immunity, the present invention also will disclose the preparation method of this health food.
The inventor according to through repetition test, comprehensive in, the doctor trained in Western medicine theory, chosen the Radix Astragali, rhodiola root, matsutake, American Ginseng safe, without any side effects and that can directly take and can strengthen the body immunity health food in order to manufacturing the present invention.When each raw material of choosing health food of the present invention carries out combination collocation, must consider effect that they can be brought into play separately and the trade-off effect that they are arranged in pairs or groups and can produce.At first; choosing the Radix Astragali is because it has the function of regulating immunity of organism; and its contained astragalus flavonid has non-oxidizability and removes the free radical effect; can make immunocyte avoid the damage of lipid peroxidation; and can change the immunocyte energetic supersession and promote the lymphocytic cell division effect; therefore it reduces as lymphocyte transformation rate immune system damage in the radiotherapy art; the neutrophil leucocyte disinfection vitality weakens etc. the certain protection effect; can effectively prevent the histocyte damage of caused by radiation in the lung cancer art; the MDA content of irradiation back tissue is descended; the SOD activity is protected; alleviate lung tissue hyperemia; consolidation; reactions such as pleaural adhesion and esophageal mucosa membrane erosion; immune function of human body there is humidification, and can improves the antineoplastic immune effect.Rhodiola root has anti-anti-anoxic and the effect of protection anoxic cardiac muscle; can increase hemochrome and red blood cell level in the blood; improve arterial partial pressure of oxygen and blood oxygen saturation; improve the oxygen supply of body tissue organ, based on this, rhodiola root can be by reducing oxygen demand; remove free radical; increase activities of antioxidant enzymes, suppress or reduce peroxidatic reaction of lipid, wait and realize its antioxidant effect from suppressing Hemorheology sex change change that anoxic causes.In addition, rhodiola root also can be regulated the non-specific and specific immune function of normal human under the cryogenic conditions, strengthens cold-resistant and anti-fatigue ability.And contained matsutake has the human body of raising hypoxia-bearing capability in the prescription, and can improve the low immunologic function of human body, make it to keep normal, and the Fructificatio Tricholomatis Matsutakis extract polysaccharide component all shows humidification to nospecific immunity and specific immunity, also have anti-stress effect simultaneously.The contained thick polysaccharide of American ginseng root has the effect of remarkable antagonism caused by cyclophosphamide body leucocyte and immune organ weight minimizing in the American Ginseng in the prescription, can promote the lymphocytic conversion of body of immunologic hypofunction, and can play humidification the nospecific immunity and the cellular immune function of body.Among the prescription of health food of the present invention, the selected Radix Astragali, rhodiola root, matsutake, American Ginseng all has the effect of good enhancing epidemic disease power, and rhodiola root and matsutake not only have anti-resisting oxygen lack, also all has the function that can strengthen the non-specific and specific immunity of human body, add the regulating action of American Ginseng to body non-specific immunity and cellular immunity function, and the Radix Astragali is to the effect of effect effect of human body antineoplastic immune and rhodiola root scavenger interior free yl, they unite use, can mutually promote, influence, the performance coordinative role reaches comprehensively and the enhancing immunity effect of system.In addition, theory of traditional Chinese medical science thinks sweet, the tepor of Huang Shi to have invigorating the spleen gas, beneficial lung qi, the function of strengthening the body resistance to consolidate the constitution; And American Ginseng hardship, cold, little sweet has supplementing qi and nourishing yin, rouses oneself the merit of vigour; Rhodiola root then has strengthening the spleen and replenishing qi, effect promoting blood circulation and removing blood stasis, and matsutake has and keeps fit as edible mushroom, the effect that beneficial stomach, qi-restoratives are set upright.Zhu Pin share, and plays the merit that reinforces the spleen to benefit the lung, reinforces vital energy, transfers smooth qi and blood altogether, to reach the purpose of qi-restoratives strengthening vital QI to eliminate pathogenic factors.Therefore, still be the comprehensive use that the theory of modern pharmacology two aspects all can be proved each component this prescription no matter from traditional traditional Chinese medical science, can reach and strengthen the immunity effect preferably.
The proportional quantity that the present invention can strengthen the health food each component of immunity is through repeatedly scientific experimentation of applicant, and human body can be according to formulating to the various indexs such as intake of the Radix Astragali, rhodiola root, matsutake, four kinds of components of American Ginseng, the weight portion of each component is counted: 600～900 parts of the Radixs Astragali, 400～600 parts of rhodiola roots, 400～600 parts of matsutakes, 100～200 parts of American Ginsengs, the optimum ratio of the each component that we are selected can be in weight portion: 700～800 parts of the Radixs Astragali, 450～550 parts of rhodiola roots, 450～550 parts of matsutakes, 130～180 parts of American Ginsengs.The best proportional quantity of each component in weight portion then is in the health food of the present invention: 750 parts of the Radixs Astragali, 500 parts of rhodiola roots, 500 parts of matsutakes, 150 parts of American Ginsengs.
The present invention can strengthen the preparation method of the health food of immunity, can be as following step:
1) it is standby to take by weighing the Radix Astragali, rhodiola root, matsutake, American Ginseng;
2) rhodiola root is placed 4～5 times be that 45～95% ethanol soaks, extracts, filters to its weight, weight concentration, alcohol extract; Reclaim the ethanol in the alcohol extract then, again it is concentrated, get the rhodiola root thick paste; The soak time of wherein said rhodiola root was generally 3～5 hours, and extraction time was generally 1～2 hour, and extraction time is generally 1～3 time;
3) matsutake is placed 6～8 times of water soak, decoct, get the water extract to its weight; The filtered water extract concentrates filtrate more then, gets the matsutake thick paste; The soak time of wherein said matsutake was generally 3～5 hours, and extraction time was generally 1～2 hour, and extraction time is generally 1～3 time;
4) Radix Astragali is placed 6～8 times of water soak, decoct, get the water extract to its weight; Filtered water extract then, and filtrate concentrated, Radix Astragali thick paste; The soak time of the wherein said Radix Astragali was generally 3～5 hours, and extraction time was generally 1～2 hour, and extraction time is generally 1～3 time;
5) American Ginseng is cleaned, placed 50～70 ℃ of oven dry, be ground into the American Ginseng fine powder;
6) rhodiola root thick paste, matsutake thick paste, Radix Astragali thick paste, the American Ginseng fine powder with gained mixes, drying, and packing gets finished product.
Certainly also can directly adopt the extract of the Radix Astragali, rhodiola root, matsutake to add American Ginseng and together prepare the health food that the present invention can strengthen immunity, its each component can be in weight portion: 300～400 parts of Astragalus Root P.Es, 300～400 parts of gadol extracts, 300～400 parts of Fructificatio Tricholomatis Matsutakis extracts, 100～200 parts of American Ginsengs; And our preferred each component proportional quantity is counted with weight portion: 320～380 parts of Astragalus Root P.Es, 320～380 parts of gadol extracts, 320～380 parts of Fructificatio Tricholomatis Matsutakis extracts, 120～180 parts of American Ginsengs; The best proportioning of each component in weight portion then is in the health food of the present invention: 350 parts of Astragalus Root P.Es, 350 parts of gadol extracts, 350 parts of Fructificatio Tricholomatis Matsutakis extracts, 150 parts of American Ginsengs.
Wherein, the described Radix Astragali, rhodiola root, Fructificatio Tricholomatis Matsutakis extract can be the commercially available Radix Astragali, rhodiola root, Fructificatio Tricholomatis Matsutakis extracts, the Astragalus Root P.E produced of Zhejiang Kang Enbei pharmaceutical Co. Ltd for example, or, also can adopt the Radix Astragali, rhodiola root, the Fructificatio Tricholomatis Matsutakis extract of method for preparing with water is carried or other method is extracted the Radix Astragali, rhodiola root, Fructificatio Tricholomatis Matsutakis extract.
The health food that the present invention can strengthen immunity is to adopt the Radix Astragali, rhodiola root, matsutake, American Ginseng science formulated, its prescription rationally, each component unite use, can play anti-anti-anoxic, cold-resistant, antineoplastic immune effect to body, and the non-specific and specific immunity of adjusting human body, thereby reach comprehensive and system's enhancing immunity of organisms effect, the crowd's of enhancing immunologic hypofunction immunity.And the raw material of health food of the present invention adopts natural prodcuts or produces from natural prodcuts, so health food of the present invention is a kind of safe, without any side effects fully, and the natural health care that can take for a long time.
(4) specific embodiment:
Embodiment 1:
1) preparation Astragalus Root P.E:
Place 8 times to soak 4 hours the Radix Astragali, decocted 1.5 hours, filter to the water of its weight, water extract A, be placed on 6 times again and in the water of its weight, soaked 4 hours, decocted 1.5 hours, filtration gets water extract B; A and B are merged, carry out micro-filtration; And then it is concentrated to reduce pressure, and it is standby to get Radix Astragali thick paste;
2) preparation gadol extract:
Place 5 times to be that 70% ethanol soaked 4 hours to its weight, weight concentration rhodiola root, and extract, extraction time is 1.5 hours, filters, and merges filtrate twice; First then decompression recycling ethanol, it is concentrated to reduce pressure again, and it is standby to get the rhodiola root thick paste;
3) preparation Fructificatio Tricholomatis Matsutakis extract:
Place 6 times of water to soak 3 hours matsutake, decocted 2 hours, filter, get the water extract to its weight; Again the water extract is carried out micro-filtration, it is concentrated to reduce pressure then, and it is standby to get the matsutake thick paste;
4) Radix Astragali thick paste 300 grams, rhodiola root thick paste 300 grams, the matsutake thick paste 300 that takes by weighing with method for preparing restrains, American Ginseng 100 grams with process oven dry, pulverizing, mix, it is concentrated to reduce pressure then, promptly gets the health food ointment that the present invention can strengthen immunity.
Embodiment 2:
1) preparation Astragalus Root P.E:
Place 7 times of water to its weight to soak 5 hours the Radix Astragali, decoct 2 times, decocted 2 hours at every turn, filter, merge the water extract twice, then the water extract is carried out micro-filtration, it is concentrated it to be reduced pressure again, and it is standby to get Radix Astragali thick paste;
2) gadol extract 350 grams of take by weighing Radix Astragali thick paste 350 gram, preparing with the water extracting method with method for preparing, the Fructificatio Tricholomatis Matsutakis extract of producing with Guilin Laiyin Biological Product Co., Ltd 350 restrains, American Ginseng 150 grams through oven dry, pulverizing together mix, carry out drying under reduced pressure then, pulverize, packing promptly gets the health-care edible capsule that the present invention can strengthen immunity.
Embodiment 3:
1) it is standby to take by weighing the Radix Astragali 600 gram, rhodiola root 400 grams, matsutake 500 grams, American Ginseng 150 grams;
2) above-mentioned each component is mixed, carry out drying under reduced pressure then, pulverize, packing promptly gets the health food granule that the present invention can strengthen immunity.
Embodiment 4:
1) takes by weighing the Radix Astragali 600 grams, rhodiola root 400 grams, matsutake 400 grams, American Ginseng 100 grams.
2) rhodiola root is placed 5 times to its weight, weight concentration be 95% ethanol soak 5 hours, extract 2 times, each extraction time is 1.5 hours, filters, merges alcohol extract twice; Reclaim the ethanol in the alcohol extract then, again it is concentrated, get the rhodiola root thick paste;
3) matsutake is placed 8 times soaked 4 hours, decocted 1.5 hours, filter to the water of its weight, water extract A, be placed on 6 times again and in the water of its weight, soaked 5 hours, decocted 1 hour, filtration gets water extract B; Merge the water extract twice; Filtered water extract then, and filtrate concentrated, the matsutake thick paste;
4) Radix Astragali is placed 8 times of water soaked 5 hours, decocted 2 hours, filter, get the water extract to its weight; Then the water extract is carried out the essence filter, and filtrate is concentrated, get Radix Astragali thick paste;
5) American Ginseng is cleaned, placed 50 ℃ of oven dry down, be ground into the American Ginseng fine powder;
6) rhodiola root thick paste, matsutake thick paste, Radix Astragali thick paste, the American Ginseng fine powder with gained mixes, drying, and packing gets the health food pulvis that the present invention can strengthen immunity.
Embodiment 5:
1) it is standby to take by weighing Astragalus Root P.E 350 grams, gadol extract 380 grams, Fructificatio Tricholomatis Matsutakis extract 400 grams that Guilin Laiyin Biological Product Co., Ltd produces respectively;
2) get 150 gram American Ginsengs and clean, place 70 ℃ of oven dry down, be ground into the American Ginseng fine powder;
3) above-mentioned Astragalus Root P.E, gadol extract, Fructificatio Tricholomatis Matsutakis extract and American Ginseng fine powder are mixed, carry out compressing tablet and handle, packing gets the health food tablet that the present invention can strengthen immunity.
Experimental example:
1, the consumption of health food: embodiment 2 described the present invention can strengthen the health-care edible capsule of immunity, and 0.5g/ grain, content are the grey powder, and cool place, the preservation of dry place are put in sealing.The human oral recommended amounts is every day 2 times, each 2, and become body weight for humans press 60kg calculating, amount to dosage 0.0333g/kgbw.Getting capsule 's content tests.
2, animal used as test and grouping: 120 of cleaning level Kunming kind female mices, body weight is 18g～22g, is provided by animal technical college animal used as test plant of Agricultural University Of Hunan, the animal used as test production licence number is SCXK (Hunan) 2003-0003.Per 40 are divided into 1 group, totally three groups.Immunity I group is carried out carbon and is cleaned up experiment; Immunity II group is carried out dirty body ratio measurement, Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell experiment, delayed allergy experiment, HD50 value (HC
50) mensuration and the mensuration of antibody-producting cell number; Immunity III group is carried out mouse lymphocyte transformation experiment, NK cell activity mensuration that ConA induces.
3, health food dosage is selected and handled when experiment: according to the oral recommended amounts of human body, the basic, normal, high dosage of establishing the health-care edible capsule that the present invention can strengthen immunity is respectively 0.167g/kgbw, 0.333g/kgbw, 0.999g/kgbw (be equivalent to respectively human body RD 5,10,30 times).Get health-care edible capsule content 1.67g, 3.33g, the 9.99g adding distil water that embodiment 2 described the present invention can strengthen immunity respectively and be assigned to 200ml, the animal of not taking medicine gives isopyknic distilled water, give animal subject respectively and irritate stomach, irritate stomach every day once, irritate the long-pending 0.2ml/10gbw of being of body of stomach, continuous 30 days.
4, experimental technique:
(1) internal organs/weight ratio pH-value determination pH: mouse is put to death in the back of weighing, and takes out spleen and thymus gland, weighs on electronic analytical balance, calculates dirty/body ratio.
(2) delayed allergy (DTH) (the sufficient sole of the foot thickens method): after mouse peritoneal injection lines 2% (v/v) SRBC (the every mouse of the 0.2ml/) sensitization 4 days, measure left back sufficient sole of the foot thickness, then measuring point hypodermic injection 20% (v/v) SRBC (the every mouse of 20ul/), measure left back sufficient sole of the foot portion thickness in injection back 24h, same position is measured three times, averages.The degree of representing DTH with sufficient sole of the foot thickness difference (swelling degree of the paw) before and after attacking.
(3) the ConA mouse lymphocyte transformation experiment (mtt assay) of inducing: the aseptic spleen of getting, place the little plate that fills an amount of aseptic Hank ' s liquid, make cell suspension, filter through 200 eye mesh screens.Use Hank ' s liquid to wash 2 times, each centrifugal 10 minutes (1000r/min).Then cell is suspended in the 1mL complete culture solution, the living cell counting number, adjusting cell concentration with the RPMI1640 nutrient solution is 3 * 10
6Individual/mL.Again cell suspension is divided two holes to add in 24 well culture plates, every hole 1mL, a hole adds 75 μ LConA liquid (being equivalent to 7.5 μ g/mL) therein, and 5% carbon dioxide is put in contrast in another hole, cultivates 72h for 37 ℃.Cultivate and finish preceding 4h, supernatant 0.7mL is inhaled in every hole gently, adds the RPMI1640 nutrient solution that does not contain calf serum, adds MTT (5mg/mL) 50 μ L/ holes simultaneously, continues to cultivate 4h.After cultivating end, every hole adds 1mL acid isopropyl alcohol, and the piping and druming mixing dissolves purple crystal fully.Divide then to install in 96 well culture plates, 3 parallel holes are made in each hole, use ELIASA, measure OD value with the 570nm wavelength.Lymphocytic multiplication capacity deducts with the OD value that adds the ConA hole and does not add ConA OD value also and represent.
(4) antibody-producting cell detects (Jerne improve slide method): get sheep blood, wash 3 times with physiological saline, centrifugal at every turn (2000r/min) 10min is made into the cell suspension of 2% (v/v), every mouse lumbar injection 0.2ml with hematocrit SRBC with physiological saline.Mouse that immunity is back 4 days is put to death, and gets spleen, tears up gently, makes cell suspension with Hanks liquid, and 200 eye mesh screens filter, and washs, centrifugal 2 times, at last cell is suspended in the 5mlHanks liquid.To mix with the PH7.4 of equivalent, the Hanks liquid of 2 times of concentration after the culture medium heating for dissolving of top layer, the packing small test tube, every pipe 0.5ml, in pipe, add 10%SRBC50ul (v/v), 20ul splenocyte suspension again with the preparation of SA liquid, be poured on the slide of brushing the thin layer agarose behind the mixing rapidly, treat after agarose solidifies flat button of slide to be placed on the slide frame, put into CO2gas incubator incubation 1.5h, will join with the complement (1: 10) of SA liquid dilution and continue in the slide groove to count the hemolysis plaque number behind the incubation 1.5h.
(5) HD50 value (HC
50) mensuration: get sheep blood, with physiological saline washing 3 times, every mouse carries out immunity through lumbar injection 2% (v/v prepares with physiological saline) hematocrit RBC0.2mL.After 5 days, extract eyeball and get blood in centrifuge tube, place about 1h, solidification blood and tube wall are peeled off, right to use serum is fully separated out, and the centrifugal 10min of 2000rpm collects serum.Is 200 times with the SA buffer solution with the serum dilution, gets 1mL and puts in vitro, adds 10% (v/v is with the preparation of SA buffer solution) hematocrit SRBC0.5Ml successively, complement 1mL (pressing dilution in 1: 10 with the SA buffer solution).Other establishes the not control tube of increase serum (replacing with the SA buffer solution).After putting in 37 ℃ of waters bath with thermostatic control insulation 30min, the ice bath cessation reaction.The centrifugal 10min of 2000rpm gets supernatant 1mL, adds Dou Shi reagent 3mL.Get 10% (v/v is with the preparation of SA buffer solution) hematocrit SRBC0.25mL simultaneously, add Dou Shi reagent and measure in the test tube in other to 4mL, abundant mixing, behind the placement 10min, it is blank to sentence the control tube work in 540nm, measures respectively and respectively manages OD value.The amount of hemolysin is with HD50 value (HC
50) expression, be calculated as follows:
HD50 value (HC
50OD value * extension rate during)=sample OD value/SRBC HD50
(6) mouse carbon is cleaned up experiment: mouse tail vein injection is with the india ink of 4 times of physiological saline dilutions, every 10g body weight injection 0.1mL, and timing immediately after prepared Chinese ink injects after injecting prepared Chinese ink the 2nd, 10min, is got blood 20ul from the angular vein clump respectively, joins 2mlNa
2CO
3Solution is made blank, with 722 type spectrophotometers at 600nm wavelength place colorimetric photometry density value (OD).Mouse is put to death, get liver, spleen, weigh, calculate phagocytic index a.
(7) Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell experiment (half intracorporal method): mouse peritoneal injection 20% (v/v, prepare with physiological saline) hematocrit chicken red blood cell (2000rpm, 10min) suspension 1mL, interval 30min, the cervical vertebra dislocation is put to death, and gets peritoneal macrophage washing lotion 1mL, drips on slide, put into the enamel box that is lined with wet gauze, put 37 ℃ of incubator incubation 30min.Incubate completely, rinsing is to remove not paster cell in physiological saline.Dry, with methyl alcohol: acetone (1: 1) is fixing, and the dyeing of 4% (v/v) Giemsa-phosphate buffer is dried with the distilled water rinsing.100 macrophages of every counting under the oil mirror are calculated as follows phagocytic rate and phagocytic index:
Phagocytic rate %=engulfs macrophage number * 100 of the macrophage number/counting of chicken red blood cell
The macrophage number of chicken red blood cell sum/counting that phagocytic index=quilt is engulfed
(8) the epidemic mensuration (isotope of NK cell
3The H-TdR determination method): get the back well-grown YAC-1 cell of 24h (survival rate＞95%) that goes down to posterity by 1 * 10
6/ mL YAC-1 cell suspension adds
3H-TdR10 μ Ci carries out mark, in 37 ℃, cultivates 2h in 5% CO2gas incubator, every 30min vibration 1 time, and the cell behind the mark is resuspended in the nutrient solution with nutrient solution washing 3 times, and making cell concentration is 1 * 10
5Individual/mL.Being tried the dislocation of mouse cervical vertebra puts to death, the aseptic spleen of getting, make splenocyte suspension, use Hank ' s liquid to wash 3 times, each centrifugal 10min of 1000rpm, the RPMI1640 complete culture solution that contains 10% calf serum with 2mL suspends again, and with the blue dyeing counting of platform phenol (viable count should more than 95%), adjusting cell concentration be 1 * 10
7Individual/mL.Every hole adds 100 μ Ltriton X-100 in 96 orifice plates.Each palmitic acid is established 3 multiple holes, puts 5% carbon dioxide, cultivates 4h for 37 ℃.Get storage with the bull cell and get and combine on the glass fiber filter paper, measure per minute umber of pulse (cpm) with liquid scintillation instrument.
NK cytoactive=(1-experimental port cpm/ (the maximum release aperture cpm of blank hole cpm-)) * 100%
5, experimental result:
(1) the present invention can strengthen the influence of the health-care edible capsule of immunity to the mouse body weight, sees Table 1,2,3:
The health-care edible capsule that table 1 the present invention can strengthen immunity strengthens immunity function experiment (I) group mouse (female) body weight
| Dosage group (g/kg BW) | Number of animals (only) | Initial body weight x ± s (g) | Body weight x ± s in mid-term (g) | Body weight x ± s in latter stage (g) | Weightening finish x ± s (g) |
| 0.000 0.167 0.333 0.999 | 10 10 10 10 | 19.28±1.15 19.27±1.18 19.31±1.08 19.87±1.45 | 28.07±1.68 28.12±1.76 28.27±1.77 28.42±1.88 | 35.76±2.19 35.50±2.10 35.82±2.17 36.37±1.55 | 16.48±1.74 16.23±1.76 16.51±1.85 16.50±1.69 |
The health-care edible capsule that table 2 the present invention can strengthen immunity strengthens immunity function experiment (II) group mouse (female) body weight
| Dosage group (g/kg BW) | Number of animals (only) | Initial body weight x ± s (g) | Body weight x ± s in mid-term (g) | Body weight x ± s in latter stage (g) | Weightening finish x ± s (g) |
| 0.000 0.167 0.333 0.999 | 10 10 10 10 | 20.26±1.47 20.25±1.40 20.33±1.18 20.38±1.35 | 28.86±1.99 28.66±1.59 28.17±1.37 29.07±1.87 | 36.56±2.23 35.43±1.85 35.81±1.64 36.09±1.69 | 16.30±1.42 15.18±1.38 15.48±1.72 15.71±1.70 |
The health-care edible capsule that table 3 the present invention can strengthen immunity strengthens immunity function experiment (III) group mouse (female) body weight
| Dosage group (g/kg BW) | Number of animals (only) | Initial body weight x ± s (g) | Body weight x ± s in mid-term (g) | Body weight x ± s in latter stage (g) | Weightening finish x ± s (g) |
| 0.000 0.167 0.333 0.999 | 10 10 10 10 | 19.95±1.27 19.88±1.21 19.90±1.09 19.99±1.24 | 28.77±1.66 28.49±1.85 28.60±1.84 28.04±1.86 | 36.19±1.67 35.95±2.32 35.89±1.55 35.79±1.92 | 16.24±1.42 16.07±1.46 15.99±1.02 15.80±1.22 |
By table 1-3 as seen, each dosage group is tested just, is tested mid-term, the last mouse body weight of experiment and experimental session weight of mice control group relatively, and difference does not have conspicuousness (P＞0.05)
(2) the present invention can strengthen the influence of the health-care edible capsule of immunity to mouse immune organ internal organs/body weight ratio, sees Table 4:
Table 4 the present invention can strengthen the influence of the health-care edible capsule of immunity to mouse immune organ internal organs/body weight ratio
| Dosage group (g/kg BW) | Number of animals (only) | Spleen/body weight x ± s (%) | The P value | Thymus gland/body weight x ± s (%) | The P value |
| 0.000 0.167 0.333 0.999 | 10 10 10 10 | 0.479±0.028 0.492±0.032 0.507±0.036 0.516±0.043 | --- 0.752 0.199 0.063 | 0.284±0.043 0.302±0.052 0.315±0.075 0.311±0.039 | --- 0.806 0.447 0.542 |
By table 4 as seen, the present invention that per os gives the mouse various dose can strengthen the health-care edible capsule content 30 days of immunity, to mouse spleen/body weight ratio and thymus gland/body weight ratio do not make significant difference (P＞0.05)
(3) the present invention can strengthen immunity the health-care edible capsule capsule to the mouse cell Immune Effects, see Table 5,6:
A: the present invention can strengthen the influence of the health-care edible capsule of immunity to mouse delayed allergy (DTH), sees Table 5:
Table 5 the present invention can strengthen the influence of the health-care edible capsule of immunity to mouse delayed allergy (DTH)
| Dosage group (g/kg BW) | Number of animals (only) | Injection back 24h swelling degree of the paw x ± s (mm) | The P value |
| 0.000 0.167 0.333 0.999 | 10 10 10 10 | 0.275±0.066 0.313±0.064 0.340±0.054 0.351±0.036 | --- 0.315 0.036 0.012 |
As shown in Table 5, the present invention that per os gives the mouse various dose can strengthen the health-care edible capsule content 30 days of immunity, and middle and high dosage group swelling degree of the paw is apparently higher than control group, and difference has conspicuousness (P＞0.05).
B: the influence of the mouse lymphocyte conversion capability experiment that the health-care edible capsule that the present invention can strengthen immunity is induced mouse ConA sees Table 6:
Table 6 the present invention can strengthen the influence of the health-care edible capsule of immunity to the experiment of mouse lymphocyte conversion capability
| Dosage group (g/kg BW) | Number of animals (only) | Lymphopoiesis ability (OD difference) x ± s | The P value |
| 0.000 0.167 0.333 0.999 | 10 10 10 10 | 0.053±0.017 0.080±0.054 0.074±0.029 0.070±0.036 | --- 0.271 0.461 0.620 |
By table 6 as seen, the present invention that per os gives the mouse various dose can strengthen the health-care edible capsule content 30 days of immunity, each dosage group mouse lymphocyte conversion capability and control group relatively, difference does not have conspicuousness (P＞0.05).
(4) the present invention can strengthen the influence of the health-care edible capsule of immunity to humoral immunity, sees Table 7,8:
A: the present invention can strengthen the influence of the health-care edible capsule of immunity to mouse antibodies cellulation number, sees Table 7:
Table 7 the present invention can strengthen the influence of the health-care edible capsule of immunity to mouse antibodies cellulation number
| Dosage group (g/kg BW) | Number of animals (only) | Hemolysis plaque is counted x ± s (* 10 3/ full spleen) | The P value |
| 0.000 0.167 0.333 0.999 | 10 10 10 10 | 22.6±8.5 30.4±11.8 38.9±10.6 46.6±18.8 | …… 0.404 0.022 0.001 |
As shown in Table 7, the present invention that per os gives the mouse various dose can strengthen the health-care edible capsule content 30 days of immunity, and middle and high dosage group mouse antibodies cellulation number is apparently higher than control group, and difference has conspicuousness (P＜0.05 or P＜0.01).
B: the present invention can strengthen the health-care edible capsule of immunity to mouse HD50 value (HC
50) influence, see Table 8:
Table 8 the present invention can strengthen the influence of the health-care edible capsule of immunity to mouse HD50 value (HC50)
| Dosage group (g/kg BW) | Number of animals (only) | HD50 value x ± s | The P value |
| 0.000 0.167 0.333 0.999 | 10 10 10 10 | 181.05±28.73 197.28±18.63 201.99±18.23 220.11±30.46 | …… 0.334 0.159 0.003 |
By table 8 as seen, the present invention that per os gives the mouse various dose can strengthen the health-care edible capsule content 30 days of immunity, high dose group mouse HD50 value with according to group relatively, difference has conspicuousness (P＜0.01)
(5) the present invention can strengthen the influence of the health-care edible capsule of immunity to mouse monokaryon one macrophage phagocytic function, sees Table 9,10,11:
A: the present invention can strengthen the influence that the health-care edible capsule of immunity is cleaned up mouse monokaryon one macrophage carbon, sees Table 9:
Table 9 the present invention can strengthen the influence that the health-care edible capsule of immunity is cleaned up mouse monokaryon one macrophage carbon
| Dosage group (g/kg BW) | Number of animals (only) | Phagocytic index (a) x ± s | The P value |
| 0.000 0.167 0.333 0.999 | 10 10 10 10 | 5.538±0.851 6.055±0.550 6.411±0.769 6.534±0.818 | --- 0.304 0.037 0.015 |
By table 9 as seen, the present invention that per os gives the mouse various dose can strengthen the health-care edible capsule content 30 days of immunity, and middle and high dosage group mouse phagocytic index is significantly higher than control group, and difference has conspicuousness (P＜0.05).
B: the health-care edible capsule that the present invention can strengthen immunity is engulfed the influence of chicken red blood cell ability to mouse macrophage, sees Table 10,11:
Table 10 the present invention can strengthen the health-care edible capsule of immunity is engulfed the chicken red blood cell phagocytic rate to mouse macrophage influence
| Dosage group (g/kg BW) | Number of animals (only) | Phagocytic rate x ± s (%) | Phagocytic rate square root arcsine conversion value x ± s | The P value |
| 0.000 0.167 0.333 0.999 | 10 10 10 10 | 28.2±7.6 29.6±7.5 34.0±8.5 34.9±7.0 | 31.9±4.8 32.8±4.7 35.5±5.2 36.1±4.2 | --- 0.950 0.227 0.133 |
The health-care edible capsule that table 11 the present invention can strengthen immunity is engulfed the influence of chicken red blood cell phagocytic index to mouse macrophage
| Dosage group (g/kg BW) | Number of animals (only) | Phagocytic index x ± s | The P value |
| 0.000 0.167 0.333 0.999 | 10 10 10 10 | 0.929±0.184 0.988±0.212 1.075±0.257 1.086±0.186 | --- 0.868 0.300 0.247 |
By table 10-11 as seen, the present invention that per os gives the mouse various dose can strengthen the health-care edible capsule content 30 days of immunity, and each dosage group is engulfed the chicken red blood cell ability to mouse macrophage and do not seen obvious influence.
(6) the present invention can strengthen the influence of the health-care edible capsule of immunity to the NK cells in mice activity, sees Table 12:
Table 12 the present invention can strengthen the influence of the health-care edible capsule of immunity to the NK cells in mice activity
| Dosage group (g/kg BW) | Number of animals (only) | NK cytoactive x ± s (%) | NK cytoactive square root arcsine conversion value x ± s | The P value |
| 0.000 0.167 0.333 0.999 | 10 10 10 10 | 34.0±11.3 38.0±7.9 38.2±12.6 46.4±16.3 | 35.4±7.1 37.9±5.0 38.0±7.5 42.9±9.7 | --- 0.792 0.771 0.079 |
By table 12 as seen, the present invention that per os gives the mouse various dose can strengthen the health-care edible capsule content 30 days of immunity, each dosage group to NK cells in mice active with control group relatively, difference does not have conspicuousness (P＞0.05).
6, experiment conclusion:
The present invention that per os gives mouse 0.167g/kgbw, 0.333g/kgbw, 0.999g/kgbw dosage can strengthen the health-care edible capsule content 30 days of immunity, 0.333g/kgbw, 0.999g/kgbw dosage can strengthen the mouse delayed allergy, improve the antibody-producting cell number of mouse, improve the ability that mouse monokaryon one macrophage carbon is cleaned up, 0.999g/kgbw dosage can improve mice serum HD50 value.Compare P＜0.05 or P＜0.01 with control group.The ability of mouse lymphocyte conversion capability that weight of mice, spleen/body weight ratio, thymus gland/body weight ratio, ConA are induced and NK cells in mice activity, mouse macrophage chicken red blood cell does not have obvious influence.The health food that prompting the present invention can strengthen immunity has the function that strengthens immunity.
Claims (9)
1, a kind of health food that can strengthen immunity is characterized in that each component counts with weight portion: 100～200 parts of 600～900 parts of the Radixs Astragali, 400～600 parts of rhodiola roots, 400～600 parts of matsutakes and American Ginsengs.
2, the health food that can strengthen immunity according to claim 1 is characterized in that each component counts with weight portion: 130～180 parts of 700～800 parts of the Radixs Astragali, 450～550 parts of rhodiola roots, 450～550 parts of matsutakes and American Ginsengs.
3, the health food that can strengthen immunity according to claim 2 is characterized in that each component counts with weight portion: 150 parts of 750 parts of the Radixs Astragali, 500 parts of rhodiola roots, 500 parts of matsutakes and American Ginsengs.
4, according to any one described preparation method that can strengthen the health food of immunity in the claim 1～3, its step is as follows:
1) it is standby to take by weighing the Radix Astragali, rhodiola root, matsutake, American Ginseng;
2) rhodiola root is placed 4～5 times be that 45～95% ethanol soaks, extracts, filters to its weight, weight concentration, alcohol extract; Reclaim the ethanol in the alcohol extract then, again it is concentrated, get the rhodiola root thick paste;
3) matsutake is placed 6～8 times of water soak, decoct, get the water extract to its weight; Filtered water extract then, and filtrate concentrated, the matsutake thick paste;
4) Radix Astragali is placed 6～8 times of water soak, decoct, get the water extract to its weight; Filtered water extract then, and filtrate concentrated, Radix Astragali thick paste;
5) American Ginseng is cleaned, placed 50～70 ℃ of oven dry, be ground into the American Ginseng fine powder;
6) rhodiola root thick paste, matsutake thick paste, Radix Astragali thick paste, the American Ginseng fine powder with gained mixes, drying, and packing gets finished product.
5, the preparation method that can strengthen the health food of immunity according to claim 4 is characterized in that:
Step 2) in, the soak time of rhodiola root is 3～5 hours, and extraction time is 1～2 hour, and extraction time is 1～3 time;
In the step 3), the soak time of matsutake is 3～5 hours, and extraction time is 1～2 hour, and extraction time is 1～3 time;
In the step 4), the soak time of the Radix Astragali is 3～5 hours, and extraction time is 1～2 hour, and extraction time is 1～3 time.
6, a kind of health food that can strengthen immunity, it is characterized in that each component counts with weight portion: 100～200 parts of 300～400 parts of Astragalus Root P.Es, 300～400 parts of gadol extracts, 300～400 parts of Fructificatio Tricholomatis Matsutakis extracts and American Ginsengs, the preparation method of wherein said gadol extract, as following step:
1) rhodiola root is placed 4～5 times be that 45～95% ethanol soaks, extracts, filters to its weight, weight concentration, alcohol extract;
2) ethanol in the recovery alcohol extract, and it is concentrated, get gadol extract;
The preparation method of wherein said Fructificatio Tricholomatis Matsutakis extract, as following step:
1) matsutake is placed 6～8 times of water soak, decoct, get the water extract to weight;
2) filtered water extract;
3) filtrate is concentrated, get Fructificatio Tricholomatis Matsutakis extract;
The preparation method of wherein said Astragalus Root P.E, as following step:
1) Radix Astragali is placed 6～8 times of water soak, decoct, get the water extract to weight;
2) filtered water extract;
3) filtrate is concentrated, get Astragalus Root P.E.
7, the health food that can strengthen immunity according to claim 6 is characterized in that each component counts with weight portion: 120～180 parts of 320～380 parts of Astragalus Root P.Es, 320～380 parts of gadol extracts, 320～380 parts of Fructificatio Tricholomatis Matsutakis extracts and American Ginsengs.
8, the health food that can strengthen immunity according to claim 7 is characterized in that each component counts with weight portion: 150 parts of 350 parts of Astragalus Root P.Es, 350 parts of gadol extracts, 350 parts of Fructificatio Tricholomatis Matsutakis extracts and American Ginsengs.
9, the health food that can strengthen immunity according to claim 6 is characterized in that:
In preparation method's step 1) of gadol extract, the soak time of rhodiola root is 3～5 hours, and extraction time is 1～2 hour, and extraction time is 1～3 time;
In preparation method's step 1) of Fructificatio Tricholomatis Matsutakis extract, the soak time of matsutake is 3～5 hours, and extraction time is 1～2 hour, and extraction time is 1～3 time;
In preparation method's step 1) of Astragalus Root P.E, the soak time of the Radix Astragali is 3～5 hours, and extraction time is 1～2 hour, and extraction time is 1～3 time.
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| CN101129441B (en) * | 2007-09-20 | 2011-05-18 | 尹治华 | Rhodiola rosea capsule and its preparing method |
| CN101810318B (en) * | 2010-04-26 | 2012-10-10 | 大连盖世食品有限公司 | Tricholoma matsutake can and preparation method thereof |
| CN102641325B (en) * | 2012-04-25 | 2014-03-05 | 吉林大学珠海学院 | Health care product having function of resisting fatigue and enhancing immunity and preparation method thereof |
| CN104473234A (en) * | 2014-12-23 | 2015-04-01 | 潘建杰 | Health-care bighead carp ball food |
| CN104432239A (en) * | 2014-12-23 | 2015-03-25 | 施东德 | Sturgeon ball food |
| CN104489681A (en) * | 2014-12-30 | 2015-04-08 | 广州健码医药生物科技有限公司 | Composition with function of enhancing immunity, health product and preparation method thereof |
| CN104840717A (en) * | 2015-05-20 | 2015-08-19 | 罗堂烈 | Medicinal liquor capable of enriching essence, invigorating kidney and enhancing immunity |
| CN107495367B (en) * | 2017-08-24 | 2020-11-20 | 四川省中医药科学院 | Food, health-care product or pharmaceutical composition for improving anoxia resistance and preparation method and application thereof |
| CN108450940A (en) * | 2018-02-09 | 2018-08-28 | 甘孜州康定蓝生态科技有限责任公司 | Lower hyperlipidemia, hypertension, hyperglycemia, radioresistance tumour, the health products and preparation method for improving immunity |
| CN109363175A (en) * | 2018-11-01 | 2019-02-22 | 云南香绿源科技开发有限公司 | A kind of functionality integration of drinking and medicinal herbs food and preparation method thereof |
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| CN1429622A (en) * | 2003-01-18 | 2003-07-16 | 郝基荣 | Radix rhodiolae double action lozenge |
| CN1463712A (en) * | 2002-06-06 | 2003-12-31 | 吴高雄 | Health food additive and process for preparing same |
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| CN1463712A (en) * | 2002-06-06 | 2003-12-31 | 吴高雄 | Health food additive and process for preparing same |
| CN1429622A (en) * | 2003-01-18 | 2003-07-16 | 郝基荣 | Radix rhodiolae double action lozenge |
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