CN110448586A - A kind of Astragalus Root P.E and its preparation method and application - Google Patents
A kind of Astragalus Root P.E and its preparation method and application Download PDFInfo
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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Abstract
The present invention provides a kind of Astragalus Root P.Es and its preparation method and application, belong to tcm field.The Astragalus Root P.E includes following components by weight: raw 20~60 parts of Radix Astragali, and 49~100 parts of preparing astragalus membranaceus, 40~60 parts of glutinous rice, 0.8~1.2 part of red yeast rice, 0.1~0.5 part of sweet Wine yeast, 0.1~0.3 part of citric acid；Wherein, the weight ratio of honey and raw Radix Astragali is 9~20:40~80 in the preparing astragalus membranaceus.Astragalus Root P.E of the invention is after being processed, decocted through honey by raw Radix Astragali, to mix with the life Radix Astragali directly decocted, and glutinous rice, red yeast rice, sweet Wine yeast, citric acid etc. is added to repeat ferment, separate, being spray-dried.Astragalus Root P.E provided by the invention is used to prepare immunopotentiating agent or regulator and growth promoter, has the function of the immunoloregulation function and growth promotion more more efficient than existing astragalus polyose product, and the present invention solves the problems, such as that existing astragalus polyose is easily got angry very well.
Description
Technical field
The invention belongs to technical field of Chinese medicine, and in particular to a kind of Astragalus Root P.E and its preparation method and application.
Background technique
Traditional Chinese medicine Radix Astragali is the medicine of help, has the effect of invigorating qi for consolidating superficies, arrest sweating admittedly take off, torr sore myogenic, inducing diuresis for removing edema,
For treating deficiency of vital energy and acking in strength, the sinking of qi of middle-jiao, rush down prolapse of the anus for a long time, uterine bleeding of having blood in stool, exterior deficiency spontaneous perspiration, ulcer is difficult to burst, burst for a long time and does not holds back, the deficiency of blood withers
Huang, Heat Diabetes, chronic nephritis, albuminuria, diabetes etc..Tcm clinical practice is used as medicine with Radix Astragali, need to be according to the needs of diagnosis and treatment based on an overall analysis of the illness and the patient's condition
Corresponding processed product is processed or is selected accordingly, due to the difference of processing procedure, different Radix Astragali processed product functions have not again
Together.For example, raw Radix Astragali is good at strengthening exterior and reducing sweat, inducing diuresis for removing edema, pus draining and toxin expelling is chiefly used in defensive Qi instability, and when spontaneous perspiration is made, physically weak sense
It emits, oedema, sore difficulty is burst etc.；Sweet Radix Astragali invigorating middle warmer and replenishing QI is chiefly used in deficiency of vital energy and acking in strength, anorexia and loose stool person；Stir-bake to yellowish stilbene invigorate the spleen and benefit qi and
Do not stop up stagnant, controls splenasthenic abdominal distention, anorexia and loose stool；Wine astragalus root temperature rise effect is stronger, is suitable for deficiency of vital energy lung cold, collapse from qi deficiency；Etc..
Astragalus polyose is water solubility heteroglycan made of extracted Milkvetch Root, concentration, purifying, by hexuronic acid, grape
The composition such as sugar, fructose, rhamnose, arabinose, galacturonic acid and glucuronic acid, it is clinical usually as immunopotentiating agent or
Regulator uses, while being also used for antiviral and antitumor, anti-aging, anti-radiation, resisting stress, anti-oxidant etc..Existing Radix Astragali is more
Sugared extractive technique since medicinal material used is made a living Radix Astragali, take decoct extract, the mode of concentration, drying, resulting polyose is difficult
To reach the diversified application demand of different Radix Astragali processed products, clinical application discovery, long-time service easily leads to the excessive internal heat such as " constipation "
Symptom occurs.
In order to change the single status of current this astragalus polyose products application, reduce or avoid existing astragalus polyose to be easy
Cause the defect got angry, needs to innovate existing astragalus polyose extraction process, to obtain the Huang of more multifunctional characteristics
Stilbene extract, to meet the different demands according to Traditional Chinese Medicine medication.
Summary of the invention
The object of the invention is in order to solve the above-mentioned technical problem, and provide a kind of Astragalus Root P.E and its preparation side
Method and application not only solve astragalus polyose and are easy the characteristics of getting angry, and gained Astragalus Root P.E has good immune function,
The property easy to use of astragalus polyose is improved, while having the characteristics that the medicinal of the astragalus root prepared slices of Chinese crude drugs again, livestock and poultry can be promoted raw
It is long, and drug effect greatly enhances, side effect substantially reduces！Therefore, the present invention be to provide it is a kind of can strengthen immunity and promote livestock and poultry
Growth and the Astragalus Root P.E that do not get angry.
An object of the present invention is to provide a kind of Astragalus Root P.E, the Astragalus Root P.E by weight, including with
Lower component: raw 20~60 parts of Radix Astragali, 49~100 parts of preparing astragalus membranaceus, 40~60 parts of glutinous rice, 0.8~1.2 part of red yeast rice, sweet Wine yeast
0.1~0.5 part, 0.1~0.3 part of citric acid；Wherein, the preparing astragalus membranaceus is to be carried out frying by honey and raw Radix Astragali and obtained, honey
The weight ratio of honey and raw Radix Astragali is 9~20:40~80 in astragalus root.
Above-mentioned formula of the invention provides a kind of sweet Radix Astragali (processed) extractive from fermentative, is to be processed, decocted through honey by raw Radix Astragali
After boiling, mixed with the life Radix Astragali directly decocted, then plus glutinous rice, red yeast rice, sweet Wine yeast, citric acid etc. repeat fermentation, separation, spray
Mist drying forms.In order to be distinguished with existing astragalus polyose or Astragalus Root P.E, hereinafter " sweet stilbene ferment powder ".It is more with existing Radix Astragali
Sugar or Astragalus Root P.E are compared, and sweet stilbene ferment powder of the invention has stronger drug effect and lower side effect, can be greatly enhanced
Immunocompetence promotes growth of animals or poultry, and sweet stilbene ferment powder provided by the invention has the characteristics that not get angry.
As a preferred option, astragalus extraction composition formula of the present invention is as follows: by weight, including following components:
Raw 60 parts of Radix Astragali, 49 parts of preparing astragalus membranaceus, 60 parts of glutinous rice, 0.8 part of red yeast rice, 0.5 part of sweet Wine yeast, 0.1 part of citric acid；Wherein, sweet
The parts by weight of honey are 9 parts in astragalus root, and the parts by weight of raw Radix Astragali are 40 parts.Using currently preferred formula, gained
Astragalus Root P.E product quality is more preferably.
The second object of the present invention is to provide the preparation method of above-mentioned Astragalus Root P.E (i.e. " sweet stilbene ferment powder "), the preparation
Method the following steps are included:
(1) the corresponding parts by weight of preparing astragalus membranaceus raw material are pressed, honey is taken, equivalent amount of water is added to stir evenly, be uniformly sprayed at raw Radix Astragali
On, it is absorbed into it dry；Then it is placed in frying pan and carries out mild fire frying, until astragalus mongholicus tablet surface is in brown color, adds 5 times of amount water, boil
Boiling, cools to certain temperature；
(2) corresponding parts by weight are pressed, raw Radix Astragali is taken, adds 5 times of amount water, boil, cool to certain temperature；
(3) corresponding parts by weight are pressed, glutinous rice is taken, adds bubble saturating, cook, cool to certain temperature；
(4) corresponding parts by weight are pressed, red yeast rice, sweet Wine yeast and citric acid are taken, with step (1), step (2) and step (3) institute
It obtaining object to be mixed, then sealed fermenting 72 hours, filtering takes filtered fluid spare, adds the cold water isometric with filtrate,
Sealed fermenting 48 hours, filtering, take filtrate spare, then plus the cold water isometric with filtrate, sealed fermenting 24 hours, filtering,
Take filtrate；The filtrate that 3 times are obtained merges, and concentration, spray drying to moisture is lower than 1% to get Astragalus Root P.E (" sweet stilbene ferment
Powder ").
Medicinal material used in existing astragalus polyose extractive technique is made a living Radix Astragali, generally take be decoct extract, concentration, drying
Mode, this resulting polyose of extracting mode is difficult to reach the diversified application demand of different Radix Astragali processed products, clinical
Using discovery, long-time service often results in the excessive internal heats symptom such as " constipation " appearance.And preparation process of the invention is by raw Radix Astragali through honey
Toast after decocting, mix with the life Radix Astragali directly decocted, then plus glutinous rice, red yeast rice, sweet Wine yeast, citric acid etc. repeat fermentation, divide
From, be spray-dried, " the sweet stilbene ferment powder " that above-mentioned technique according to the invention is prepared, be not in easy in use process
The problem of excessive internal heat, and being verified by experiments, is either still used for piglet for chick, in terms of improve immunity obtained by the present invention
" sweet stilbene ferment powder " is superior to existing astragalus polyose.Meanwhile relatively existing astragalus polyose, the present invention, which further increases, promotes chicken growth
And reduce the function of feed-weight ratio.
As a preferred option, the weight ratio of honey described in step (1) and raw Radix Astragali is 0.9:4.
As a preferred option, the parts by weight of raw Radix Astragali described in step (2) are 60 parts.
As a preferred option, the parts by weight of raw material described in step (3) and step (4) are as follows: 60 parts of glutinous rice, red yeast rice 0.8
Part, 0.5 part of sweet Wine yeast, 0.1 part of citric acid.
Further, the temperature of frying described in step (1) is 120~150 DEG C.
Further, the temperature after cooling described in step (1)-(3) is 20 DEG C~40 DEG C.
The third object of the present invention is to provide application of the Astragalus Root P.E as described above in terms of field of medicaments, is to be used for
Prepare the application in terms of immunopotentiating agent or regulator and livestock and poultry growth promotion.
Compared with prior art, beneficial effects of the present invention are as follows:
The present invention is to provide related sweet Radix Astragali (processed) extractive from fermentative and its preparation and application, with existing astragalus polyose or
Astragalus Root P.E has larger difference, has stronger drug effect and lower side effect, has its immunocompetence and greatly enhance, and have
It is not easy the property got angry.It is verified by experiments, either the influence to the white granulocyte percentage of neutrophilia in piglet blood or right
The influence of piglet hog cholera antibody level, piglet lymphocyte transformation rate, it is more that the sweet stilbene ferment powder of present invention gained is superior to existing Radix Astragali
Sugar.
It is verified by experiments, is either still used for piglet for chick, the honey stilbene obtained by the present invention in terms of improve immunity
Ferment powder is superior to existing astragalus polyose.Meanwhile relatively existing astragalus polyose, the present invention, which further increases, promotes chicken growth and drop
The function of low feed-weight ratio.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments to the present invention
It is specifically described, it is necessary to, it is noted that following embodiment is used only for that the present invention is explained and illustrated, be not used to
Limit the present invention.Some nonessential modifications and adaptations that those skilled in the art are made according to foregoing invention content, still belong to
In protection scope of the present invention.
Embodiment 1
Material: red yeast rice (Hebei Lin Shi prepared slices of Chinese crude drugs Co., Ltd)；Raw Radix Astragali (Cologne prepared slices of Chinese crude drugs Co., Ltd, Palestine and China)；
Sweet Wine yeast (Angel Yeast Co., Ltd)；Citric acid, honey, glutinous rice (commercially available).
Technique: taking 1 part of honey, equivalent amount of water added to stir evenly, and is uniformly sprayed on 10 parts of raw Radix Astragalis, absorbs to it complete
(to dry)；The life Radix Astragali of hydromel will be added to be placed in frying pan, (120 DEG C~150 DEG C) of mild fire are fried to astragalus mongholicus tablet surface in pale brown
Color adds 5 times of amount water, boils, and cools to room temperature (20 DEG C~40 DEG C)；5 parts of glutinous rice are taken, adds bubble saturating, cooks, is cooled to room temperature；
0.1 part of red yeast rice, 0.01 part of sweet Wine yeast, 0.01 part of citric acid are taken, is mixed with above-mentioned the two, sealed fermenting 72 hours, filtering takes
Liquid is spare, then plus the cold water isometric with filtrate, sealed fermenting 48 hours, filtering took liquid spare, then plus with filtrate etc.
The cold water of volume, sealed fermenting 24 hours, filtering took liquid；The filtrate that 3 times are obtained merges, concentration, spray drying to water
Divide lower than 1% to get sweet stilbene ferment powder A.
Embodiment 2
Material: raw Radix Astragali (Cologne prepared slices of Chinese crude drugs Co., Ltd, Palestine and China)；Sweet Wine yeast (Angel Yeast Co., Ltd)；
Citric acid, honey, glutinous rice (commercially available).
1 part of honey is taken, equivalent amount of water is added to stir evenly, is uniformly sprayed on 10 parts of raw Radix Astragalis, to its absorption；Honey will have been added
In the life Radix Astragali merging frying pan of water, astragalus mongholicus tablet surface is fried with slow fire in brown color, adds 5 times of amount water, boils, cool to room temperature；It takes
5 parts of glutinous rice, add bubble saturating, cook, cools to room temperature；0.01 part of sweet Wine yeast, 0.01 part of citric acid are taken, it is mixed with above-mentioned the two
It is even, sealed fermenting 72 hours, filtering, take liquid spare, then plus the cold water isometric with filtrate, sealed fermenting 48 hours, mistake
Filter, take liquid spare, then plus the cold water isometric with filtrate, sealed fermenting 24 hours, filtering, take liquid；3 times are obtained
Filtrate merges, and concentration, spray drying to moisture is lower than 1% to get sweet stilbene ferment powder B.
Embodiment 3
Material: red yeast rice (Hebei Lin Shi prepared slices of Chinese crude drugs Co., Ltd)；Raw Radix Astragali (the limited public affairs of Palestine and China's Cologne prepared slices of Chinese crude drugs
Department)；Citric acid, honey, glutinous rice (commercially available).
1 part of honey is taken, equivalent amount of water is added to stir evenly, is uniformly sprayed on 10 parts of raw Radix Astragalis, to its absorption；Honey will have been added
In the life Radix Astragali merging frying pan of water, astragalus mongholicus tablet surface is fried with slow fire in brown color, adds 5 times of amount water, boils, cool to room temperature；It takes
5 parts of glutinous rice, add bubble saturating, cook, cools to room temperature；0.1 part of red yeast rice, 0.01 part of citric acid are taken, is mixed with above-mentioned the two, it is closed
Fermentation 72 hours, filtering, take liquid spare, then plus the cold water isometric with filtrate, sealed fermenting 48 hours, filtering, take liquid
Body is spare, then plus the cold water isometric with filtrate, sealed fermenting 24 hours, filtering, take liquid；The filtrate that 3 times are obtained is closed
And be concentrated, spray drying to moisture is lower than 1% to get sweet stilbene ferment powder C.
Embodiment 4
Material: red yeast rice (Hebei Lin Shi prepared slices of Chinese crude drugs Co., Ltd)；Raw Radix Astragali (the limited public affairs of Palestine and China's Cologne prepared slices of Chinese crude drugs
Department)；Sweet Wine yeast (Angel Yeast Co., Ltd)；Citric acid, glutinous rice (commercially available).
It takes on 10 parts of raw Radix Astragalis, adds 5 times of amount water, boil, cool to room temperature；5 parts of glutinous rice are taken, adds bubble saturating, cooks, cool
To room temperature；0.1 part of red yeast rice, 0.01 part of sweet Wine yeast, 0.01 part of citric acid are taken, is mixed with above-mentioned the two, sealed fermenting 72 hours,
Filtering, take liquid spare, then plus the cold water isometric with filtrate, sealed fermenting 48 hours, filtering, take liquid spare, then plus
Isometric cold water with filtrate, sealed fermenting 24 hours, filtering took liquid；The filtrate that 3 times are obtained merges, and is concentrated, spraying
Drying is to moisture lower than 1% to get sweet stilbene ferment powder D.
Embodiment 5
Material: red yeast rice (Hebei Lin Shi prepared slices of Chinese crude drugs Co., Ltd)；Raw Radix Astragali (the limited public affairs of Palestine and China's Cologne prepared slices of Chinese crude drugs
Department)；Sweet Wine yeast (Angel Yeast Co., Ltd)；Citric acid, honey, glutinous rice (commercially available).
Technique: taking 1 part of honey, equivalent amount of water added to stir evenly, and is uniformly sprayed on 5 parts of raw Radix Astragalis, to its absorption；It will add
In the life Radix Astragali merging frying pan of hydromel, astragalus mongholicus tablet surface is fried with slow fire in brown color, adds 5 times of amount water, boils, cool to room
Temperature；5 parts of raw Radix Astragali is taken, adds 5 times of amount water, boils, cool to room temperature；5 parts of glutinous rice are taken, adds bubble saturating, cooks, is cooled to room temperature；
0.1 part of red yeast rice, 0.01 part of sweet Wine yeast, 0.01 part of citric acid are taken, is mixed with above-mentioned the two, sealed fermenting 72 hours, filtering takes
Liquid is spare, then plus the cold water isometric with filtrate, sealed fermenting 48 hours, filtering took liquid spare, then plus with filtrate etc.
The cold water of volume, sealed fermenting 24 hours, filtering took liquid；The filtrate that 3 times are obtained merges, concentration, spray drying to water
Divide lower than 1% to get sweet stilbene ferment powder E.
Embodiment 6
Material: red yeast rice (Hebei Lin Shi prepared slices of Chinese crude drugs Co., Ltd)；Raw Radix Astragali (the limited public affairs of Palestine and China's Cologne prepared slices of Chinese crude drugs
Department)；Sweet Wine yeast (Angel Yeast Co., Ltd)；Honey, glutinous rice (commercially available).
Technique: taking 1 part of honey, equivalent amount of water added to stir evenly, and is uniformly sprayed on 5 parts of raw Radix Astragalis, to its absorption；It will add
In the life Radix Astragali merging frying pan of hydromel, astragalus mongholicus tablet surface is fried with slow fire in brown color, adds 5 times of amount water, boils, cool to room
Temperature；It takes raw stilbene 5 parts yellow, adds 5 times of amount water, boil, cool to room temperature；5 parts of glutinous rice are taken, adds bubble saturating, cooks, is cooled to room temperature；
0.1 part of red yeast rice, 0.01 part of sweet Wine yeast are taken, is mixed with above-mentioned the two, sealed fermenting 72 hours, filtering takes liquid spare, then plus
Isometric cold water with filtrate, sealed fermenting 48 hours, filtering, take liquid spare, then plus the cold water isometric with filtrate,
Sealed fermenting 24 hours, filtering took liquid；The filtrate that 3 times are obtained merges, and concentration, spray drying to moisture is lower than 1%, i.e.,
Obtain sweet stilbene ferment powder F.
Embodiment 7
Material: test rat 90 (Sichuan Academy of Medical Sciences's Experimental Animal Center), half male and half female；Cyclophosphamide；It is yellow
Astragalus polysaccharides (commercially available)；Sweet stilbene ferment powder A~F (Examples 1 to 6 extraction).
Rat is randomly divided into 9 groups (every group 10, half male and half female), is followed successively by blank group, negative control group, positive control
Group, test A group, test B group, test C group, test D group, test E group, test F group.In addition to blank group, each group is big during test
Mouse intraperitoneal injection of cyclophosphamide 0.1ml/d after three days, in addition to blank group and negative control group, successively adds in each group rat drinking-water
(positive controls add commercially available astragalus polyose, test A group plus sweet stilbene ferment powder A, test B group plus sweet stilbene ferment powder to 1% correspondence experiment product
B, test C group plus sweet stilbene ferment powder C, test D group plus sweet stilbene ferment powder D, test E group plus sweet stilbene ferment powder E, test F group plus sweet stilbene ferment powder
F), continuous 7 days, routine observation rat defecation situation during test judged whether there is constipation and constipation journey by visually rank
Degree, measures each group Rats Spleen index, thymus index and CD4+/CD8+ respectively, the results are shown in Table 1 after 7 days.
1 rat spleen of table, thymic weight and its index compare
From the point of view of result in table 1, the result for testing E group is best, either index and spleen index, thymus index, or immune tune
Section effect, is all substantially better than positive controls, while also avoiding astragalus polyose scorching, and long-term use causes the pairs such as constipation or diarrhea
The defect of effect.
Embodiment 8
Material: red yeast rice (Hebei Lin Shi prepared slices of Chinese crude drugs Co., Ltd)；Radix Astragali (Cologne prepared slices of Chinese crude drugs Co., Ltd, Palestine and China),
It is divided into two parts of " Radix Astragali A " and " Radix Astragali B " according to prescription needs；Sweet Wine yeast (Angel Yeast Co., Ltd)；Citric acid, bee
Honey, glutinous rice (commercially available)；It tests rat 100 (Sichuan Academy of Medical Sciences's Experimental Animal Center), half male and half female；Cyclophosphamide；
Astragalus polyose (commercially available).
Sweet stilbene ferment powder prescription preferred embodiment design table (part) of table 2
| Prescription number | Honey | Radix Astragali A | Radix Astragali B | Glutinous rice | Red yeast rice | Sweet Wine yeast | Citric acid |
| 1 | 2.5 | 9 | 1 | 3.5 | 0.13 | 0.06 | 0.04 |
| 2 | 2 | 8 | 2 | 4 | 0.12 | 0.04 | 0.03 |
| 3 | 1.5 | 7 | 3 | 4.5 | 0.11 | 0.02 | 0.02 |
| 4 | 1 | 6 | 4 | 5 | 0.1 | 0.01 | 0.01 |
| 5 | 0.95 | 5 | 5 | 5.5 | 0.09 | 0.03 | 0.01 |
| 6 | 0.9 | 4 | 6 | 6 | 0.08 | 0.05 | 0.01 |
| 7 | 0.85 | 3 | 7 | 6.5 | 0.07 | 0.07 | 0.01 |
Technique: by table 2, square gauge takes honey surely everywhere, and equivalent amount of water is added to stir evenly, and is uniformly sprayed on Radix Astragali A, to its suction
It receives；The life Radix Astragali of hydromel will have been added to be placed in frying pan, astragalus mongholicus tablet surface has been fried with slow fire in brown color, adds 5 times of amount water, boil,
It cools to room temperature；Radix Astragali B is taken, adds 5 times of amount water, boils, cool to room temperature；5 parts of glutinous rice are taken, adds bubble saturating, cooks, is cooled to room
Temperature；Add red yeast rice, sweet Wine yeast, citric acid, mix, sealed fermenting 72 hours, filtering takes liquid spare, then plus with the bodies such as filtrate
Long-pending cold water, sealed fermenting 48 hours, filtering, take liquid spare, then plus the cold water isometric with filtrate, sealed fermenting 24
Hour, filtering takes liquid；The filtrate that 3 times are obtained merges, and concentration, spray drying to moisture is lower than 1%.1~7 is prepared respectively
Number sweet stilbene ferment powder.
Rat is randomly divided into 10 groups (every group 10, half male and half female), is followed successively by 1~7 group of test, blank group, negative right
According to group, positive controls.In addition to blank group, each group rats by intraperitoneal injection cyclophosphamide 0.1ml/d is removed after three days during test
Outside blank group and negative control group, 1% 1~No. 7 sweet stilbene ferment powder, positive controls are successively added in 1~7 group of rat drinking-water of test
Add 1% astragalus polyose in rat drinking-water, continuous 7 days, routine observation rat defecation situation, was sentenced by visually rank during test
It is disconnected to whether there is constipation and constipation degree, each group Rats Spleen index, thymus index and CD4+/CD8+ are measured after 7 days respectively, is tied
Fruit is shown in Table 3.
3 rat spleen of table, thymic weight and its index compare
From the point of view of result in table 3,2~6 groups of test either index and spleen index, thymus index or immunoregulation effect, all
Positive controls are substantially better than, while will not also cause constipation or loose stool etc..
Influence of the sweet stilbene ferment powder of experimental example 1 to chick immune function
Material: 1 age in days AA+ commodity Broiler chicks 180 (certain chicken house of Chengdu)；Astragalus polyose (Xi'an rock sky biotechnology
Co., Ltd)；Sweet stilbene ferment powder (preparation of embodiment 5)；Chicken Newcastle disease live vaccine (Beijing biological products Co., Ltd, Hua Doushi China)；
Newcastle disease virus hemagglutination-inhibition test antigen (China Veterinery Drug Inspection Office).
Method: the nonimmune AA+ Broiler chicks of 180 1 ages in days are randomly divided into 3 groups, i.e. immunized controls group (does not add medicine
Object), astragalus polyose group (additive amount 200mg/kg) and sweet stilbene ferment powder group (additive amount 200mg/kg), every group of 3 repetitions, often
Repeat 20 chickens.Timing feeds, and Broiler chicks are freely eaten, free water.The immune programme of 3 groups of Broiler chicks is shown in Table 4.
4 Broiler chicks immune programme of table
| Age in days | Vaccine | Inoculation method | It measures again |
| 1~7 | Newcastle disease+biography branch | Collunarium and eye droppings | 1.2~1.5 |
| 14~16 | Infectious bursa of Fabricius | Drinking-water | 1.2 |
| 21~23 | Newcastle disease | Drinking-water | 1.2 |
Related inspection target and its investigation method and result are as follows.
Before Broiler chicks raise feeding in 1,7,14,21,28 and 35 age in days mornings, claims each group Broiler chicks empty body weight and remember
Record, records the average daily feed intake of every group of Broiler chicks respectively, and calculates Broiler chicks in the average weight (being shown in Table 5) of each age in days section and material
Again than (being shown in Table 6).
5 different days Broiler chicks average weight (g) of table
| Age in days | Immunized controls group | Astragalus polyose group | Sweet stilbene ferment powder group | Group difference |
| 1 | 47.05 | 46.69 | 47.39 | It is not significant |
| 7 | 154.03 | 155.44 | 157.04 | It is not significant |
| 14 | 348.58 | 382.02 | 389.88 | It is significant |
| 21 | 715.25 | 733.32 | 777.04 | It is significant |
| 28 | 1215.42 | 1273.46 | 1286.35 | It is significant |
| 35 | 1759.22 | 1818.86 | 1870.22 | It is significant |
As seen from Table 5, after 14 ages in days, the average weight of astragalus polyose group and sweet stilbene ferment powder group Broiler chicks is all remarkably higher than
The average weight of immunized controls group, sweet stilbene ferment powder group Broiler chicks is all remarkably higher than astragalus polyose group.
The feed-weight ratio of 6 different days Broiler chicks of table
As seen from Table 6, after 28 ages in days, the feed-weight ratio of sweet stilbene ferment powder group is substantially less than astragalus polyose group and immunized controls
Group, the feed-weight ratio of sweet stilbene ferment powder group are substantially less than astragalus polyose group.When 14,21,28 and 35 age in days, every group is taken out at random
6 chickens are taken, wing venous blood sampling separates serum, by corresponding in national standard " GB/T16550-2008 newcastle disease diagnostic techniques "
Method detects antibody titres to newcastle disease virus (the results are shown in Table 7).
Newcastle epidemic disease antibody is horizontal in 7 Broiler chicks serum of table
| Age in days | Immunized controls group | Astragalus polyose group | Sweet stilbene ferment powder group | Group difference |
| 14 | 3.77 | 3.90 | 4.38 | Significant difference between control group and experimental group |
| 21 | 4.30 | 4.57 | 4.81 | Significant difference between control group and experimental group |
| 28 | 4.89 | 5.10 | 6.47 | Sweet stilbene ferment powder group and other group of significant difference |
| 35 | 5.20 | 6.54 | 7.22 | Sweet stilbene ferment powder group and other group of significant difference |
As seen from Table 7, astragalus polyose group and sweet stilbene ferment powder group the newcastle epidemic disease antibody water mean height in serum in different days
In immunized controls group.At 14 and 21 age in days, the newcastle epidemic disease antibody level of astragalus polyose group and sweet stilbene ferment powder group is above immune
Control group, and significant difference；At 28 and 35 age in days, the newcastle epidemic disease antibody level of sweet stilbene ferment powder group is significantly higher than astragalus polyose
Group；In 35 age in days, sweet stilbene ferment powder group newcastle epidemic disease antibody level is significantly higher than other each groups.
Each group randomly selects 6 Broiler chicks when 35 age in days, dissects after claiming quality on an empty stomach, completely wins 3 kinds of immune organs:
Thymus gland, the bursa of farbricius and spleen claim Fresh Yuxincao after rejecting surface fascia at once and record, and Computation immunity shoot formation: immune organ refers to
Number=organ mass ÷ weight (the results are shown in Table 8).
Broiler chicks Immune Organs Index g/kg when 8 35 age in days of table
As seen from Table 8, the Immune Organs Index of astragalus polyose group and sweet stilbene ferment powder group Broiler chicks is significantly higher than immunized controls
Group；Sweet stilbene ferment powder group Immune Organs Index is slightly above astragalus polyose group, but difference is not significant, and the thymus index of sweet stilbene ferment powder group is high
In other each groups, and significant difference.
Before Broiler chicks raise feeding in 1,7,14,21,28 and 35 age in days mornings, each group Broiler chicks excrement is collected, using " in
State's veterinary drug allusion quotation (version in 2015) " dry weight-loss method measurement excrement water content, the dry and hard degree of (excessive internal heat causes) excrement is assessed, is as a result seen
Table 9.
The excrement loss on drying rate % of 9 different days Broiler chicks of table
| Age in days | Immunized controls group | Astragalus polyose group | Sweet stilbene ferment powder group | Group difference |
| 7 | 83.37 | 83.60 | 83.45 | It is not significant |
| 14 | 82.50 | 81.46 | 82.37 | It is not significant |
| 21 | 81.94 | 79.87 | 81.77 | It is not significant |
| 28 | 80.72 | 76.19 | 80.64 | It is significant |
| 35 | 79.37 | 70.60 | 79.70 | It is significant |
As seen from Table 9,7,14 and when 21 age in days, astragalus polyose group and sweet stilbene ferment powder group are above immunized controls group, but poor
It is different not significant；28 and when 35 age in days, the dejecta moisture of sweet stilbene ferment powder group and immunized controls group is all remarkably higher than astragalus polyose, honey
The dejecta moisture and immunized controls group of stilbene ferment powder group are without significant difference.
Influence of the sweet stilbene ferment powder of experimental example 2 to piglet immunological function
Material: 3 nest of ternary piglet similar in 20 age in days weight (10, every nest, Chengdu pig farm)；Astragalus polyose (commercially available)；
Sweet stilbene ferment powder (preparation of embodiment 5)；Hog cholera vaccine (Liaoning Yikang Biological Co., Ltd.)；Hog cholera antibody detection kit
(Lanzhou Veterinary Inst., Chinese Acedemy of Agaricultural Sciences).
Method: 3 nest, 20 age in days ternary piglet, is randomly divided into control group, astragalus polyose group, sweet stilbene by 4 parts of injecting swine fever vaccine
Ferment powder group, every group 10, the ablactation of 28 ages in days；25 ages in days start, and astragalus polyose group feeds astragalus polyose (additive amount 200mg/
Kg), sweet stilbene ferment powder group feeds sweet stilbene ferment powder (additive amount 200mg/kg)；Control group is not offerd medicine, when 25,40 and 50 age in days into
Row blood sampling, and immune index detection is carried out, it is as a result as follows.
10 astragalus polyose group of table and sweet stilbene ferment powder are to the influence % of the white granulocyte percentage of piglet blood neutrophilia
As seen from Table 10, when 25 age in days, the white granulocyte percentage of piglet neutrophilia between astragalus polyose group and sweet stilbene ferment powder group
Difference is not significant；When 40 age in days, the white granulocyte percentage of peripheral blood neutrophilia of astragalus polyose group and sweet stilbene ferment powder group is significant
Higher than control group.Sweet stilbene ferment powder group is higher than astragalus polyose group, and difference is unobvious.In 50 age in days, astragalus polyose is higher than control group,
Sweet stilbene ferment powder group is significantly higher than astragalus polyose group.
The influence of 11 astragalus polyose of table and sweet stilbene ferment powder to piglet hog cholera antibody level
As seen from Table 11,40 and when 50 age in days, astragalus polyose group and sweet stilbene ferment powder group hog cholera antibody level are all remarkably higher than
Control group.In 40 age in days, astragalus polyose group and sweet stilbene ferment powder group are not significantly different.When 50 age in days, sweet stilbene ferment powder group is significant
Higher than astragalus polyose group.The result shows that astragalus polyose group and sweet stilbene ferment powder group piglet hog cholera antibody level difference in 25 age in days
It is not significant, but with the increase of raising age in days, at 40 and 50 age in days, sweet stilbene ferment powder group hog cholera antibody level is not only significantly higher than
Control group, also significantly greater than astragalus polyose group.It can be seen that astragalus polyose, sweet stilbene ferment powder all have the immune function of piglet
Certain facilitation, but still there is better immunological enhancement after the application of sweet stilbene ferment powder.
The influence of 12 astragalus polyose of table and sweet stilbene ferment powder to piglet lymphocyte transformation rate
| Age in days | Control group | Astragalus polyose group | Sweet stilbene ferment powder group |
| 25 | 33.975 | 33.873 | 33.441 |
| 40 | 38.263 | 41.701 | 42.767 |
| 50 | 42.875 | 45.726 | 47.394 |
As can be seen from Table 12, piglet periphery lymphocyte conversion ratio is in 25 age in days, astragalus polyose group and sweet stilbene ferment powder
Equal difference is not significant between group and control group；When 40 ages in days and 50 age in days, sweet stilbene ferment powder group is extremely significant to be higher than control group, remaining each group
Between difference it is not significant.The result shows that astragalus polyose and sweet stilbene ferment powder can be improved the conversion ratio of piglet lymphocyte, to improve
The immune function of piglet, and with sweet stilbene ferment powder group conversion ratio highest.
In conclusion the influence either to the white granulocyte percentage of neutrophilia in piglet blood, or to piglet swine fever
The influence of antibody level, piglet lymphocyte transformation rate, the sweet stilbene ferment powder of present invention gained are superior to astragalus polyose.
Claims (9)
1. a kind of energy strengthen immunity and the Astragalus Root P.E for promoting growth of animals or poultry and not getting angry, which is characterized in that the Radix Astragali
Extract by weight, including following components: raw 20~60 parts of Radix Astragali, 49~100 parts of preparing astragalus membranaceus, 40~60 parts of glutinous rice,
0.8~1.2 part of red yeast rice, 0.1~0.5 part of sweet Wine yeast, 0.1~0.3 part of citric acid；Wherein, the preparing astragalus membranaceus is by honey
Frying is carried out with raw Radix Astragali and is obtained, and the weight ratio of honey and raw Radix Astragali is 9~20:40~80 in preparing astragalus membranaceus.
2. according to claim 1 strengthen immunity and growth of animals or poultry and the Astragalus Root P.E do not got angry, feature can be promoted
Be, the Astragalus Root P.E by weight, including following components: raw 60 parts of Radix Astragali, 49 parts of preparing astragalus membranaceus, 60 parts of glutinous rice,
0.8 part of red yeast rice, 0.5 part of sweet Wine yeast, 0.1 part of citric acid；Wherein, the parts by weight of honey are 9 parts in preparing astragalus membranaceus, raw Radix Astragali
Parts by weight be 40 parts.
3. a kind of preparation of energy strengthen immunity and promotion growth of animals or poultry and the Astragalus Root P.E that do not get angry as described in claim 1
Method, which comprises the following steps:
(1) the corresponding parts by weight of preparing astragalus membranaceus raw material are pressed, honey is taken, equivalent amount of water is added to stir evenly, be uniformly sprayed on raw Radix Astragali, to
It is absorbed into dry；Then it is placed in frying pan and carries out mild fire frying, until astragalus mongholicus tablet surface is in brown color, adds 5 times of amount water, boil, put
It is cool to certain temperature；
(2) corresponding parts by weight are pressed, raw Radix Astragali is taken, adds 5 times of amount water, boil, cool to certain temperature；
(3) corresponding parts by weight are pressed, glutinous rice is taken, adds bubble saturating, cook, cool to certain temperature；
(4) corresponding parts by weight are pressed, red yeast rice, sweet Wine yeast and citric acid are taken, with step (1), step (2) and step (3) gains
It is mixed, then sealed fermenting 72 hours, filters, take filtered fluid spare, add the cold water isometric with filtrate, it is closed
Fermentation 48 hours, filtering, take filtrate spare, then plus the cold water isometric with filtrate, sealed fermenting 24 hours, filtering, take filter
Liquid；The filtrate that 3 times are obtained merges, and concentration, spray drying to moisture is lower than 1% to get Astragalus Root P.E.
4. preparation method according to claim 3, which is characterized in that the weight of honey described in step (1) and raw Radix Astragali
Than for 0.9:4.
5. preparation method according to claim 3, which is characterized in that the parts by weight of raw Radix Astragali described in step (2) are 60
Part.
6. preparation method according to claim 3, which is characterized in that raw material described in the step (3) and step (4)
Parts by weight are as follows: 60 parts of glutinous rice, 0.8 part of red yeast rice, 0.5 part of sweet Wine yeast, 0.1 part of citric acid.
7. preparation method according to claim 3, which is characterized in that the temperature of frying described in step (1) be 120~
150℃。
8. preparation method according to claim 3, which is characterized in that the temperature after cooling described in step (1)-(3) is
20 DEG C~40 DEG C.
9. any one of Astragalus Root P.E or claim 3-8 the method are prepared as described in claim any one of 1-2
The application of Astragalus Root P.E, which is characterized in that be to be applied to prepare immunopotentiating agent or regulator and livestock and poultry growth promotion
The application of aspect.
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| CN112980630A (en) * | 2020-12-22 | 2021-06-18 | 江西中医药大学 | Astragalus root fresh sweet rice wine with anti-fatigue effect and preparation method thereof |
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|---|---|---|---|---|
| CN102102067A (en) * | 2009-12-18 | 2011-06-22 | 施凤芬 | Production process of astragalus liquor |
| CN104224900A (en) * | 2013-06-14 | 2014-12-24 | 铜仁梵净山中药饮片有限公司 | Preparation process of astragalus root fried with honey |
| CN108938714A (en) * | 2018-08-29 | 2018-12-07 | 成都乾坤动物药业股份有限公司 | Ferment Milkvetch root composition and its preparation method and application |
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2019
- 2019-08-14 CN CN201910750680.1A patent/CN110448586A/en active Pending
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| CN102102067A (en) * | 2009-12-18 | 2011-06-22 | 施凤芬 | Production process of astragalus liquor |
| CN104224900A (en) * | 2013-06-14 | 2014-12-24 | 铜仁梵净山中药饮片有限公司 | Preparation process of astragalus root fried with honey |
| CN108938714A (en) * | 2018-08-29 | 2018-12-07 | 成都乾坤动物药业股份有限公司 | Ferment Milkvetch root composition and its preparation method and application |
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| CN112980630A (en) * | 2020-12-22 | 2021-06-18 | 江西中医药大学 | Astragalus root fresh sweet rice wine with anti-fatigue effect and preparation method thereof |
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