CN101322738A - Preparation of red sandalwood extract for treating diabetic and uses thereof - Google Patents
Preparation of red sandalwood extract for treating diabetic and uses thereof Download PDFInfo
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- CN101322738A CN101322738A CNA2007101109444A CN200710110944A CN101322738A CN 101322738 A CN101322738 A CN 101322738A CN A2007101109444 A CNA2007101109444 A CN A2007101109444A CN 200710110944 A CN200710110944 A CN 200710110944A CN 101322738 A CN101322738 A CN 101322738A
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Abstract
The invention relates to a red sandalwood extract for treating diabetes and the application of the extract in drugs or health products for treating diabetes. The preparation method is that red sandalwood powder is soaked in different solvents (water or 95% ethanol or n-hexane) in different glass bottles for two days, the solution is filtered, and the steps are repeated for three to four times until the extract is colorless. The extract is distilled and condensed in a manner of decompression, frozen and dried, thus obtaining the red sandalwood extract. The red sandalwood extract obtained is added with proper auxiliary materials by the routine method of pharmaceutics and made into the drugs or health products in the dosage form of powder injection, drop, troche, capsule, oral liquid, granule, etc.
Description
Technical field:
The present invention relates to a kind of red sandalwood extract for the treatment of diabetes and reach, its this extract is in the medicine of treatment diabetes or the application in the health product.
Summary of the invention:
Diabetes are a kind of chronic diseases, characteristics are hyperglycemia, because absolute or relative insulin cyclical level deficiency causes. though different types of oral antidiabetic drug may be used for the treatment of diabetes with insulin, people more and more are concerned about medical herbs, because side effect and these therapeutic agents are because their significant effect, the clinical experience of minimal side effect and relative cheap cost, prescriptions of Chinese medicine extensive use.
Diabetes are the metabolic endocrinopathy of a common pilosity, change and aged tendency of population along with expanding economy and human life style, diabetics mainly is that type ii diabetes patient's quantity increases sharply, its sickness rate in the world especially developing country be and increase trend gradually, and claim fashion trend.At present, diabetes have become the commonly encountered diseases frequently-occurring disease of the many countries in the whole world, and its mortality rate has occupied the 3rd after tumor, the cardiovascular diseases, are to be only second to the disease that need give priority to after cancer, acquired immune deficiency syndrome (AIDS) and the cardiovascular in the industrially developed country.
Diabetes belong to " diabetes " category in motherland's Chinese medicine, become thin with polydipsia, polyphagia, polyuria, health, or turbid urine, urine is pleasantly sweet is its Clinical symptoms, and it is scorching that pathogenesis is mainly the deficiency of YIN.Modern medicine roughly is divided into two types of insulin-dependent (I) and non-insulin-depending types (II) by its pathological characters with diabetes, the former mainly is because the checking of the beta Cell of islet autoimmunity due to viral infection, the chemical substance in the nature-nurture factor, cause mainly treating clinically due to β cytoclasis, functional lesion, the insulin secretion disappearance with the supplemented with exogenous insulin; The latter is then relevant with the relative property shortage of insulin resistant and insulin secretion, and both are homogeneity.Type ii diabetes generally has the use insulin preparation, often by keeping on a diet or taking the too high blood sugar level of hypoglycemic medicine control patient.At present, Chang Yong orally-taken blood sugar reducing chemical drugs is mainly sulphur (acyl) ureas, biguanides and phlorose glycoside enzyme inhibitor clinically, but these medicines all exist serious adverse reaction, particularly intestinal side effect, or take the back curative effect for a long time and do not add even defective such as invalid.Simultaneously, though the Chinese traditional compound medicine of some treatment diabetes is also arranged during the domestic market at present, exist deficiencies such as curative effect is not obvious, the cycle of taking is long mostly.
Lignum pterocarpi indici " botanical term be red sandalwood, be the pulse family Pterocarpus.Latin is called Pterocarpus santalinusL.F, and commodity are called red sanders, red sandal-wood.
Its timber can make body shrink, to augment and diaphoresis. the timber of pasty state is as cold therapy inflammation and headache. it is said that this is to help treat depression and dermatosis. the medicine of enduring out is as astringent cardiac tonic chronic dysentery bark powder gruel, be to be used for the treatment of diarrhoea. to drink and Lignum pterocarpi indici water every day be used for the treatment of diabetes twice. the locality is also referred to as rakthaehandan. other timber and barks with generic, and Pterocarpus marsupium Roxb. P.marsupium is on record hypoglycemic activity.
Li Shizhen (1518-1593 A.D.) Compendium of Material Medica our department of the Ming Dynasty the 34 volume is said: " Lignum pterocarpi indici-salty is slightly cold, and is nontoxic, energy and ying-qi detumescence poison.Available this sawdust is fried in shallow oil water and is smeared the affected part; And have relax through invigorate blood circulation, blood circulation promoting and blood pressure lowering effect.But present stage is used for the treatment of the medicine or the health product of diabetes with red sandalwood extract, yet there are no report.
The object of the present invention is achieved like this:
1, extracting Lignum pterocarpi indici tree powder is soaked in the different solvent (water or 95% ethanol or normal hexane), in the different glass bottle 2 days, and solution is filtered, three to four times so repeatedly, there is not color up to extract. the mode by blood pressure lowering is distilled extract concentrated, and lyophilization, get the Lignum pterocarpi indici extract.
2, with the Lignum pterocarpi indici extract that obtains, the practice of pharmacy with conventional adds suitable adjuvant, makes medicine or health product such as injectable powder, drop, tablet, capsule, oral liquid, granule.
The scope of the Lignum pterocarpi indici extract that 3, adds among the present invention is 0.1g to 15g
Pharmacological testing scheme of the present invention
One, hypoglycemic activity experiment
1 experiment material
1.1 medicine and reagent
1.1 medicine and reagent
Lignum pterocarpi indici water extract, Lignum pterocarpi indici ethanol extract, Lignum pterocarpi indici hexane extract, the adrenalin hydrochloride injection, the glucose alloxan, blood glucose, T-CHOL and triglyceride are measured test kit, and the saccharifying serum albumin is measured test kit
1.2 animal
Laboratory mice
1.3 instrument
721 spectrophotometers, the LDZ-0.8 medical centrifuge
2 methods
2.1 influence to normal mouse blood sugar
Get 50 of 18-20g mices, male and female half and half are divided into 5 groups at random by sex and body weight, matched group; Blank group Lignum pterocarpi indici water extract group, Lignum pterocarpi indici ethanol extract group, Lignum pterocarpi indici hexane extract group.
Each is organized mice and according to dosage distinguishes gastric infusion 1 time on an empty stomach morning every day, and the normal matched group of raising of blank group gives blank solution 0.5%CMC-Na20ml/kg, continuous 7 days.Fasting is 12 hours after the last administration, in morning on the 8th the eye socket vein get blood, separation of serum is used the determination of glucose oxidase fasting blood sugar, the significance of t check comparable group differences.
| Group | Dosage (/kg) | Number of animals (only) | Blood glucose (mg%, X ± SD) |
| Blank group | 20ml | 10 | 100.9±21.0 |
| Matched group | 2.5g | 10 | 109.2±18.1 |
| Lignum pterocarpi indici water extract group | 0.5g | 10 | 110.3±16.3 |
| Lignum pterocarpi indici ethanol extract group | 0.5g | 10 | 98.3±22.8 |
| Lignum pterocarpi indici hexane extract group | 0.5g | 10 | 104.7±20.1 |
Experimental result shows: Lignum pterocarpi indici water extract, Lignum pterocarpi indici ethanol extract, three kinds of materials of Lignum pterocarpi indici hexane extract, gavage 7 days continuously, and normal mouse blood sugar level there is not obvious influence.
2.2 influence to mice glucose hyperglycemia
Select 120 of 20-22g mices, the male and female dual-purpose is divided into 6 groups at random by sex and body weight, 20 every group: blank group, model group, matched group, Lignum pterocarpi indici water extract group, Lignum pterocarpi indici ethanol extract group, Lignum pterocarpi indici hexane extract group.
Each treated animal is in gastric infusion 1 time on an empty stomach in morning every day, and blank group gives blank solution, continuous 8 days.Fasting is 12 hours before the last administration, and behind the last 30 minutes, except that blank group intraperitoneal injection of saline 20ml/kg, all the other respectively organized equal lumbar injection 12.5% glucose solution 20ml/kg, measuring blood sugar of blood extracting concentration behind the 30min.
| Group | Dosage (/kg) | Number of animals (only) | Blood glucose (mg%, X ± SD) |
| Blank group | 20ml | 20 | 114.4±17.3 |
| Matched group | 2.5g | 20 | 514.9±32.7 |
| Model group | 20ml | 20 | 230.4±32.1 |
| Lignum pterocarpi indici water extract group | 0.5g | 20 | 220.3±23.7 |
| Lignum pterocarpi indici ethanol extract group | 0.5g | 20 | 227.7±35.1 |
| Lignum pterocarpi indici hexane extract group | 0.5g | 20 | 290.7±20.1 |
Compare with model group,
*P＜0.01
Experimental result shows, Lignum pterocarpi indici water extract, Lignum pterocarpi indici ethanol extract, three kinds of materials of Lignum pterocarpi indici hexane extract, and continuous oral 8 days has remarkable reduction effect to glucose induced mice blood sugar increasing.
2.3 influence to mice adrenal gland disposition hyperglycemia
Select 144 of 20-22g mices, the male and female dual-purpose is divided into 6 groups at random by sex and body weight, 24 every group: dosage grouping and medication are the same.Fasting is 12 hours before the last administration.After the last administration 30 minutes,, except that blank group intraperitoneal injection of saline 20ml/kg, all the other respectively organize equal lumbar injection adrenalin hydrochloride 0.2mg/kg.Get blood after 30 minutes, separation of serum is measured blood glucose value.
| Group | Dosage (/kg) | Number of animals (only) | Blood glucose (mg%, X ± SD) |
| Blank group | 20ml | 24 | 1214±17.3 |
| Matched group | 2.5g | 24 | 623.3±52.1 |
| Model group | 20ml | 24 | 311.2±67.6 |
| Lignum pterocarpi indici water extract group | 0.5g | 24 | 290.5±55.4 |
| Lignum pterocarpi indici ethanol extract group | 0.5g | 24 | 240.7±86.1 |
| Lignum pterocarpi indici hexane extract group | 0.5g | 24 | 320.7±80.1 |
Compare with model group:
*P＜0.05,
*P＜0.01
Experimental result shows: Lignum pterocarpi indici water extract, Lignum pterocarpi indici ethanol extract, three kinds of materials of Lignum pterocarpi indici hexane extract, continuous oral 8 days, the influence that can obviously resist mice adrenal gland disposition hyperglycemia
2.4 influence to alloxan diabetes mouse blood sugar, blood fat and saccharifying serum albumin
Select 30 of 20-22g mices, the male and female dual-purpose is divided into 6 groups at random by sex and body weight, 180 every group: blank group, model group, matched group, Lignum pterocarpi indici water extract group, Lignum pterocarpi indici ethanol extract group, Lignum pterocarpi indici hexane extract group.
After water 24h is can't help in each treated animal fasting, gastric infusion according to dosage respectively, after 30 minutes, except that normal control group intraperitoneal injection of saline 20ml/kg, all the other each groups are all with the normal saline solution of the dosage lumbar injection alloxan of 200mg/kg.Irritate stomach on an empty stomach once morning every day, continuous 21 days.Fasting is 12 hours before the last administration.30 minutes eye socket veins are got blood after the administration, and separation of serum is pressed kit method and measured blood glucose, T-CHOL, triglyceride and saccharifying serum albumin concentration.
Experimental result shows, Lignum pterocarpi indici water extract, Lignum pterocarpi indici ethanol extract, three kinds of materials of Lignum pterocarpi indici hexane extract, all alloxan diabetes mouse model blood glucose, triglyceride and saccharifying serum albumin there are in various degree reduction effect, but serum total cholesterol is not had obvious influence.
Two, the pharmacological evaluation design of blood sugar lowering rat effect.
1, the medicine of test usefulness:
Prepare Lignum pterocarpi indici water extract group, Lignum pterocarpi indici ethanol extract group, Lignum pterocarpi indici hexane extract by the method for introducing above.
2, the preparation of test animal;
Bring out the diabetes male white rat, 4 months ages (body weight .180g), with aforementioned said method, intraperitoneal injects frozen water (150mg/ kg body weight). behind the fortnight, 250 milligrams of obvious hyperglycemia (fasting glucose): mouse+24.2%) is selected, being used for research. measuring blood is used with Ames and is measured agent. and all animals can be used tap water and feedstuff, and the plastics cage keeps constant temperature.
3, EXPERIMENTAL DESIGN:
The mouse of contrived experiment is divided into 11 groups; Each group is by six mouse.
The Lignum pterocarpi indici extract is suspended in distilled water, the use intubate is fed, through an all night. what normally do not have treatment does not have the mouse of treatment to feed distilled water with diabetes. and the hemostix measuring blood gets zero from tail vein behind the plant extract of nursing, by the time one, three, after five, seven to nine hours. with this mouse of 11 groups, use intubate to import 0.2g/kg's " glibenclamide "
Result of the test shows Lignum pterocarpi indici water extract group, Lignum pterocarpi indici ethanol extract group, Lignum pterocarpi indici hexane extract, to normal mouse, and the effect of no blood sugar lowering, and the mouse of diabetes is had significant hypoglycemic activity.
The clinical trial design:
After top pharmacological testing screening, the Lignum pterocarpi indici extract of choosing result of the test the best is to carry out follow-up clinical trial.Select for use 28 routine patients to organize as treatment, among these patients, the age is 16～30 years old person 3 people, 31～45 years old person 5 people, and 46～60 years old person 8 people, 61～75 years old person 12 people, require: patient's fasting glucose is more than 10mmol/L, and glucose in urine is 50～100mmol/L.Choose identical example and treatment group patient's age, medical history simultaneously, patient that the state of an illness is suitable organizes in contrast, proves curative effect of medication of the present invention with this.
During the treatment, treatment group patient other any medicines of stopping using only adopt the medicine among the present invention, take night and morning, and each dose is 5 grams, and the patient of matched group takes D-860 glyburide medicine, and the instructions of taking by specification of this medicine requires to take.Treatment group and matched group are all treated and were observed three months, do inspection again.
The therapeutic effect that can draw medicine of the present invention from The above results obviously is better than the therapeutic effect of D-860 glyburide.
Claims (5)
1, a kind of medicine for the treatment of diabetes is characterized in that: described medicine adopts Lignum pterocarpi indici or its to belong to thing together and prepares with pharmacy acceptable additive and/or auxiliary agent.
2, as right 1 described a kind of medicine or health product for the treatment of diabetes, it is characterized in that: describedly belong to all floristics that thing is the pulse family Pterocarpus together, as India Lignum pterocarpi indici, Pterocarpus marsupium Roxb., kiaat etc.
3, a kind of medicine or health product for the treatment of diabetes as claimed in claim 1 is characterized in that: in described 1kg medicine or health product, contain Lignum pterocarpi indici or its extract that belongs to thing together of 0.1g to 1.5g.
4, a kind of medicine or health product for the treatment of diabetes as claimed in claim 1 is characterized in that, extracting the used appearance agent of Lignum pterocarpi indici is that agent is held in extraction commonly used on water, ethanol, hexane or other pharmaceutics.
5, a kind of medicine for the treatment of diabetes as claimed in claim 1 is characterized in that: the dosage form of described medicine adopts any dosage form of approving on the pharmaceutics.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2007101109444A CN101322738A (en) | 2007-06-11 | 2007-06-11 | Preparation of red sandalwood extract for treating diabetic and uses thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101109444A CN101322738A (en) | 2007-06-11 | 2007-06-11 | Preparation of red sandalwood extract for treating diabetic and uses thereof |
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| CN101322738A true CN101322738A (en) | 2008-12-17 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102321501A (en) * | 2011-08-24 | 2012-01-18 | 华宝食用香精香料（上海）有限公司 | A kind of process for extracting of santal characteristic perfume material |
| CN103181937A (en) * | 2011-12-30 | 2013-07-03 | 新昌县冠阳技术开发有限公司 | Antibacterial and antiviral sandalwood oil enteric-coated capsules |
| CN104448917A (en) * | 2014-12-22 | 2015-03-25 | 长沙理工大学 | Method for extracting loropetalum chinense flower red pigment by adopting acetic acid-microwave process |
| CN104479398A (en) * | 2014-12-16 | 2015-04-01 | 常州美胜生物材料有限公司 | Pterocarpus santalinus extract and preparation method and application thereof |
| CN113736567A (en) * | 2021-09-09 | 2021-12-03 | 广州大学 | Preparation method of sandalwood essential oil |
-
2007
- 2007-06-11 CN CNA2007101109444A patent/CN101322738A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102321501A (en) * | 2011-08-24 | 2012-01-18 | 华宝食用香精香料（上海）有限公司 | A kind of process for extracting of santal characteristic perfume material |
| CN103181937A (en) * | 2011-12-30 | 2013-07-03 | 新昌县冠阳技术开发有限公司 | Antibacterial and antiviral sandalwood oil enteric-coated capsules |
| CN104479398A (en) * | 2014-12-16 | 2015-04-01 | 常州美胜生物材料有限公司 | Pterocarpus santalinus extract and preparation method and application thereof |
| CN104448917A (en) * | 2014-12-22 | 2015-03-25 | 长沙理工大学 | Method for extracting loropetalum chinense flower red pigment by adopting acetic acid-microwave process |
| CN104448917B (en) * | 2014-12-22 | 2016-05-11 | 长沙理工大学 | A kind of method that adopts acetic acid-microwave technique to extract the red wooden carthamin that continues |
| CN113736567A (en) * | 2021-09-09 | 2021-12-03 | 广州大学 | Preparation method of sandalwood essential oil |
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Application publication date: 20081217 |