JP5932783B2 - Wrinkle improving composition containing placenta-derived component - Google Patents

Description

  The present invention relates to a composition for improving wrinkles comprising a component derived from a placenta such as a pig (particularly, a liquid fraction of a high-pressure treated product of placenta) as an active ingredient.

  Wrinkles are one of the aging phenomena that increase with aging. However, wrinkles are caused by many events other than those caused by aging, such as a decrease in skin water content and skin tissue damage caused by ultraviolet rays. Wrinkles due to these causes are a major issue in beauty.

  Therefore, many studies have been made on compositions that improve wrinkles. For example, a method for providing a cosmetic set for improving wrinkles by simultaneously acting an external preparation for skin and an oral composition (Patent Document 1), and a compound or extract having an astaxanthin and dermal matrix component stabilizing action A composition having an anti-wrinkle action comprising a plurality of component compositions such as a wrinkle improving composition (Patent Document 2) has been reported. Many techniques for combining placenta extract (placenta extract) with a composition containing multiple components have been reported. However, placenta extract is used for whitening, metabolic activation, cell activation, active oxygen removal, wounds, etc. It is formulated as an active ingredient having a restorative action and the placenta extract is not used as an active ingredient for improving wrinkles.

  In addition, a method for reducing wrinkles in a model animal irradiated with ultraviolet rays using a composition comprising a water-soluble component obtained by hydrolyzing pig or horse placenta with an enzyme and polyphenol (Patent Document 3), human placenta A wrinkle / wrinkle improving paper characterized by making the extract into a paper form (Patent Document 4), and a beauty composition characterized by blending neuraminic acids and / or placenta extract components into cells / tissues / growth factors. Although a method (patent document 5) used for the purpose of improving slackness has been reported, a wrinkle improving method with a clearer effect is desired.

  On the other hand, as a technique for producing a food composition or the like, a technique for processing a raw material under high pressure is known. Patent Document 6 discloses that food materials containing proteolytic enzymes promote the action of enzymes while suppressing the growth of microorganisms by holding a pressure of 50 to 100 MPa in a temperature range of 40 to 60 ° C. A method for producing a seasoning by an enzymatic decomposition method is disclosed.

Japanese Patent No. 4666537 JP 2011-63563 A JP 2002-212046 A JP 2004-182706 A JP 2009-234980 Japanese Patent No. 3475328

  The subject of this invention is providing the composition for wrinkle improvement which has a moisturizing effect and has the outstanding wrinkle improvement effect.

  As a result of intensive studies to solve the above problems, the present inventors have obtained a liquid fraction (placenta extract) containing hyaluronic acid, which is a moisturizing component, by high-pressure treatment of the placenta, and the liquid fraction It has been found that the minute has a moisturizing action and has an excellent wrinkle improving effect, and has led to the completion of the present invention.

  The present invention includes the following inventions.

(1) A wrinkle improving composition containing a liquid fraction of a high-pressure treated placenta or a treated product of the fraction.

(2) The composition according to (1), containing 0.02 w / w% or more of a liquid fraction of the high-pressure treated placenta or hyaluronic acid derived from the treated fraction.

(3) The composition according to (1) or (2), wherein the pressure of the high-pressure treatment is 50 to 150 MPa.

(4) A wrinkle improving composition containing a placenta-derived component,
The component is a component having a nitrogen weight of 0.08 or more with respect to a solid content weight of 1, and 0.02 w / w% or more of the hyaluronic acid derived from the component with respect to the total amount of the composition. The above-mentioned composition containing a solid content of 0.5 w / w% or more.

(5) The composition according to (4), wherein the placenta-derived component is a liquid fraction of a high-pressure treated product of placenta or a treated product of the fraction.

(6) The composition according to (5), wherein the pressure of the high-pressure treatment is 50 to 150 MPa.

(7) The composition according to any one of (1) to (6), which is a composition for improving wrinkles for imparting moisture to skin and making wrinkles due to drying inconspicuous.

(8) Cosmetics or pharmaceuticals (including quasi drugs) comprising the composition according to any one of (1) to (6).

(9) Use of the liquid fraction of the high-pressure treated placenta or the treated product of the fraction for the production of a composition for improving wrinkles.

(10) Use of the liquid fraction of the high-pressure treated placenta or the treated product of the fraction for wrinkle improvement.

(11) A method for improving wrinkles by applying a liquid fraction of a placental high-pressure treated product or a treated product of the fraction to the skin for cosmetic purposes.

  This application claims the priority of the Japan patent application 2011-111299 for which it applied on May 18, 2011, and includes the content described in the specification of this patent application.

  The composition for improving wrinkles of the present invention has an effect of making facial wrinkles inconspicuous by a moisturizing action, and is useful in cosmetics.

FIG. 1 shows changes with time in the amount of horny water in the eye corners of the group (n = 21) to which the composition for improving wrinkles (high-pressure extraction placenta extract) of the present invention is applied and the non-application group (n = 21). FIG. 2 shows changes over time in the wrinkle grade by visual evaluation of the group (n = 21) to which the composition for improving wrinkles (high-pressure extraction placenta extract) of the present invention was applied and the non-application group (n = 21).

  Examples of the placenta that can be used as a raw material in the present invention include placenta such as pigs and horses, with pig placenta being preferred. The breed of pig is not particularly limited. The placenta is appropriately washed and pulverized prior to processing such as high pressure processing.

  The pressure of the high-pressure treatment is not particularly limited, but is typically 50 to 150 MPa, preferably 50 to 100 MPa, more preferably 100 MPa. The temperature during the high-pressure treatment is not particularly limited and may be appropriately selected depending on the condition of the placenta and the like, but typically ranges from 45 to 65 ° C. The time for the high pressure treatment is not particularly limited, but is typically in the range of 1 to 24 hours. An apparatus for performing the high-pressure treatment is not particularly limited, and for example, an apparatus described in Patent Document 6 can be used.

  A solid fraction is separated and removed from a high-pressure treated product of the placenta by ordinary solid-liquid separation means such as centrifugation, filtration, and pressing to obtain a liquid fraction (extract). The liquid fraction may be subjected to treatment such as steroid hormone removal treatment as necessary. Since the liquid fraction itself can be used as a placenta extract, in this specification, the liquid fraction separated from the high-pressure treated placenta is referred to as “placenta extract”, “extract” or “high-pressure extraction placenta extract”. There is a case. 70-90% of the placenta used as a raw material can be recovered as a liquid fraction.

  In the present invention, “processed product of liquid fraction” means a concentrate concentrated by vacuum distillation or the like of a liquid fraction, a dried product (including powdered dried product) dried by a freeze-drying method, etc. And a diluted product obtained by diluting a concentrated product or a dried product with a solvent such as water. An extract obtained by further extracting the liquid fraction with a solvent such as water or an organic solvent is also included in the processed product of the fraction.

  The liquid fraction of the high-pressure treated placenta or the treated product thereof has a higher nitrogen content and free amino acid content than the extract extracted with purified water, and contains hyaluronic acid. This is considered to be because the growth of microorganisms is suppressed by high-pressure treatment, the cells are crushed by pressure, and the components that are usually difficult to dissolve in water are solubilized. The liquid fraction of the high-pressure treated placenta by the above procedure or the method for producing the treated fraction does not include enzymatic degradation by addition of proteolytic enzymes such as pepsin or hydrolysis by heat, acid, alkali, etc. It is preferable because a useful component (for example, a useful polypeptide (a component in which a plurality of amino acids having a molecular weight smaller than that of a protein is bound)) is less likely to be degraded or denatured. The liquid fraction of the placenta high-pressure treated product by the above procedure or the method of producing the treated product of the fraction is different from the extraction method by the freeze-thaw method in which the placenta is frozen and thawed. This is advantageous in that the microorganisms and the like are less likely to propagate in the extract.

  The composition for improving wrinkles of the present invention contains a liquid fraction of a high-pressure treated placenta or a treated product of the fraction as an active ingredient, and is provided in various forms that exhibit a moisturizing action and a wrinkle improving action. be able to. The liquid fraction of the placental high-pressure treated product or the treated product itself of the fraction can also be used as a wrinkle-improving composition. The composition for improving wrinkles of the present invention is preferably used as an external preparation for skin, and can be provided in the form of cosmetics, quasi drugs, pharmaceuticals and the like.

The external preparation for skin can be formulated using ingredients usually used for the preparation of cosmetics, quasi drugs and pharmaceuticals. The wrinkle improving composition of the present invention may further contain other active ingredients. Examples of ingredients for formulation and other active ingredients include purified water, alcohols, oily substances, moisturizers, thickeners, preservatives, emulsifiers, herbal medicine ingredients, ultraviolet absorbers, dyes, whitening agents, perfumes, and emulsifiers. Stabilizers, pH adjusters and the like can be mentioned. The dosage form of the external preparation for skin is not limited, and may be any form such as liquid, cream, ointment, lotion, gel, pack or stick, but is preferably liquid. Examples of the external skin preparation include, but are not limited to, lotion, milky lotion, gel, cosmetic liquid, general cream, pack, mask, facial cleanser, cosmetic soap, foundation, body lotion and the like. . In addition, the blending amount of the composition for improving wrinkles of the present invention in the above-mentioned various forms of products may be an amount that can exhibit the effect of improving wrinkles. What is necessary is just to set suitably in consideration of an effect, cost, etc.

  In addition, the composition for improving wrinkles of the present invention may be used not only as an external preparation for skin but also as an oral preparation, and is incorporated into foods and drinks, preferably beauty drinks and beauty health foods for the purpose of improving wrinkles. You can also.

  In the composition for improving wrinkles of the present invention, the liquid fraction of the high-pressure treated placenta used as an active ingredient or the treated product of this fraction has a nitrogen weight of 0.08 or more, preferably 1 solid content (evaporation residue). Is preferably a component of 0.10 to 0.15. In addition, the composition for improving wrinkles of the present invention has a hyaluronic acid derived from the active ingredient of 0.02 w / w% or more, preferably 0.03-0.12 w / w%, derived from the active ingredient, based on the total amount of the composition. The solid content is preferably 0.5 w / w% or more, preferably 0.55 to 1.0 w / w%, more preferably 0.60 to 0.65 w / w%.

  Furthermore, it is not limited to the embodiment using the liquid fraction of the high-pressure treated product of placenta or the treated product of the fraction as an active ingredient, and it has a composition similar to that described above and contains a placenta-derived component as an active ingredient. Compositions are also included within the scope of the present invention. The “placenta-derived component” refers to a composition (placenta processed product) obtained by treating the placenta, and may contain a plurality of types of substances. That is, the present invention relates to a placenta-derived component having a nitrogen weight of 0.08 or more, preferably 0.10 to 0.15 relative to the solid content (evaporation residue) weight of 1, and 0.02 w of hyaluronic acid derived from the component with respect to the total amount of the composition. / w% or more, preferably 0.03 to 0.12 w / w%, solid content derived from the above components is 0.5 w / w% or more, preferably 0.55 to 1.0 w / w%, more preferably 0.60 to 0.65 w / w% A composition for improving wrinkles is provided.

  The wrinkle-improving composition of the present invention has an effect of imparting moisture to the skin, and making wrinkles due to drying inconspicuous among the wrinkle-improving effects.

  EXAMPLES Hereinafter, although this invention is demonstrated based on an Example, the scope of the present invention is not limited to an Example.

(Example 1) Production and component analysis of high-pressure extraction placenta extract and evaluation test experiment 1: Production method of high-pressure extraction placenta extract (product of the present invention)
Thaw 2770 g of placenta obtained from a healthy pig of 3-4 months of gestation and thoroughly wash and remove blood etc. with low temperature purified water. The washed placenta is crushed with a Waring blender. The crushed placenta is placed in a polyethylene bag and processed at 100 MPa for 16 hours at 50 ° C. using a high-pressure processing device (manufactured by Toyo Koatsu Co., Ltd.).

  The high-pressure treated porcine placenta was centrifuged at 5000 rpm for 30 minutes at 5 ° C. to separate the solid content, and 2210 g of the supernatant was recovered. This supernatant was filtered through a 0.2 μm filter (manufactured by Nippon Pole Co., Ltd.) and subjected to steroid hormone removal treatment to recover 1760 g as a high-pressure extraction placenta extract.

Experiment 2: Manufacturing method of water-soluble placenta extract (comparative product)
Thaw 100 kg of placenta obtained from a healthy pig of 3 to 4 months of gestation, and thoroughly wash and remove blood and the like with low-temperature purified water. The washed placenta is crushed with a Waring blender. Add 10 kg of purified water to the ground placenta, and stir and extract under low temperature.

  The porcine placenta that had been subjected to the stirring and extraction treatment was centrifuged at 5000 rpm for 30 minutes at 5 ° C. to separate the solids, and the supernatant was collected. The supernatant was purified with a purification microfiltration filter or the like until the odor was reduced, and then steroid hormone removal treatment was performed to recover approximately 80 kg as a water-soluble placenta extract.

Evaluation test 1: Component comparison test of high-pressure extraction placenta extract and water-soluble placenta extract As components of high-pressure extraction placenta extract and water-soluble placenta extract, evaporation residue (solid content), total nitrogen amount (Japanese Pharmacopoeia General Test Method Nitrogen Determination Method) and the amount of hyaluronic acid (ELISA method, hyaluronic acid measurement kit, Seikagaku Biobusiness Co., Ltd.) were quantitatively analyzed and are shown in Table 1. Free amino acids were also analyzed and are shown in Table 2.

  As a result of these analyses, it was found that the high-pressure extraction placenta extract has a high nitrogen content ratio and a high free amino acid ratio in the solid content. A large amount of placenta-derived hyaluronic acid was detected in the high-pressure placenta extract. From these facts, the high-pressure extraction placenta extract is considered to be a placenta extract that contains abundant amino acids and also contains hyaluronic acid and can be expected to have a moisturizing action on the skin.

Evaluation test 2: Human moisturizing and anti-wrinkle test Wrinkle grade standard photos (8 levels of wrinkle grade 0-7) (Japan Cosmetics Society anti-wrinkle product evaluation guidelines Vol.30, No.4 , pp.316-332 (2006)), and a healthy female panel of 21 subjects with wrinkle grades 2 to 3 was used.

  The test was conducted in accordance with the anti-wrinkle product evaluation guidelines of the Japanese Cosmetic Society. The high-pressure extraction placenta extract of the present invention, which is a specimen, was applied to the periphery of the subject's eye corners three times a day (once, about 220 mg) and continued for 8 weeks. The other half face did not apply the sample. Visual evaluation was performed at 0, 4, and 8 weeks, and the amount of horny water in the area of the corner of the eye was measured.

<Evaluation of wrinkles>
Visual evaluation of wrinkles was performed on the test start date, 4 weeks and 8 weeks after the test start (the test end date). Wrinkle grade evaluation was determined with 0.5 between each grade.

Wrinkle grade Grade 0: No wrinkle grade 1: Slightly unclear shallow wrinkles are observed Grade 2: Clear shallow wrinkles are slightly observed Grade 3: Clear shallow wrinkles are observed Grade 4: Clear shallow Slightly deep wrinkles are observed in grade 5: Slightly deep wrinkles are observed Grade 6: Clear deep wrinkles are observed Grade 7: Extremely deep wrinkles are observed <Evaluation of moisture retention>
Moisture retention of the specimens was measured by Corneometer CM825 (Courage-Khazaka, Germany) on the test start date, 4 weeks and 8 weeks after the test start (study end date) using the Corneometer CM825 (Courage-Khazaka, Germany). Verified. The result was expressed by the difference between the coated part and the non-coated part. It means that there is moisture retention, so that a numerical value is large.

<Evaluation results>
The evaluation results are shown in Table 3, FIG. 1 and FIG. In the graphs of FIGS. 1 and 2, error bars indicate standard errors. Four weeks after the start of the test, the keratin water content was higher in 18 of 21 subjects than in the case of no application, and the wrinkle grade around the eye corners was improved in 12 of 21 subjects. This result is p <0.01 in the Wilcoxon test. It was found that the high-pressure extraction placenta extract of the present invention has a moisturizing action and has a significant anti-wrinkle improving action. Usually, since the anti-wrinkle effect is not significantly observed in 4 weeks, the product of the present invention is considered to have a very high effect. Furthermore, a clear effect was confirmed in the 8th week.

(Example 2) Examination of high-pressure extraction treatment conditions
(1) Examination of pressure conditions
Using a placenta prepared in the same manner as in Example 1, the extraction temperature was 50 ° C., the extraction time was 16 hours, and extraction was performed at each pressure in the range of 25 to 600 MPa. Quantitative analysis of the evaporation residue (hereinafter referred to as “solid content concentration”) of each extract (placenta extract) obtained was performed, and the results are shown in Table 4. In addition, quantitative analysis of hyaluronic acid amount and total nitrogen amount of each extract was also performed, and the results are shown in Table 4. The results were expressed as relative values, with the solid content concentration, hyaluronic acid amount, and total nitrogen amount of the extract at an extraction pressure of 100 MPa, an extraction temperature of 50 ° C., and an extraction time of 16 hours.

  The solid content concentration in the extract was highest at 100 MPa, and no significant difference was observed between 50 and 300 MPa. It was confirmed that hyaluronic acid and nitrogen were contained in any of the extracts.

  In addition, Table 5 shows the pH of each obtained placenta extract.

  The pH of the extract did not change significantly at 25 to 150 MPa, but increased at 300 MPa or more.

  Considering each analysis result of the above extract and the apparatus used for high pressure treatment, the pressure condition for the high pressure treatment of placenta extract is appropriately 50 to 150 MPa.

(2) Examination of temperature conditions
Using a placenta prepared in the same manner as in Example 1, the extraction pressure was 100 MPa, the extraction time was 16 hours, and extraction was performed at each temperature in the range of 40 to 75 ° C. Table 6 shows the solid concentration of each of the obtained extracts. In addition, Table 6 shows the hyaluronic acid amount and total nitrogen amount of each extract. The results were expressed as relative values, with the solid content concentration, hyaluronic acid amount, and total nitrogen amount of the extract at an extraction pressure of 100 MPa, an extraction temperature of 50 ° C., and an extraction time of 16 hours.

  It was confirmed that hyaluronic acid and nitrogen were contained in any of the extracts. The solid content concentration in the extract was the highest at 60 ° C, and it was considered that the range of 45 ° C to 65 ° C was appropriate as the temperature condition for the high-pressure treatment of placenta extract.

(3) Examination of time conditions
Using a placenta prepared in the same manner as in Example 1, the extraction pressure was 100 MPa, the extraction temperature was 50 ° C., and extraction was performed in each time range of 2 to 48 hours. Table 7 shows the solid content concentration of each extract obtained. In addition, Table 7 shows the hyaluronic acid amount and total nitrogen amount of each extract. The results were expressed as relative values, with the solid content concentration, hyaluronic acid amount, and total nitrogen amount of the extract at an extraction pressure of 100 MPa, an extraction temperature of 50 ° C., and an extraction time of 16 hours.

  It was confirmed that hyaluronic acid and nitrogen were contained in any of the extracts. It was confirmed that the solid content concentration in the extract increased as the treatment time in high-pressure extraction increased. However, since the extraction efficiency in units of time decreases as the processing time becomes longer, it is considered that 24 hours or less is preferable in consideration of work efficiency and operation time.

INDUSTRIAL APPLICABILITY The present invention can be used in the field of manufacturing cosmetics, pharmaceuticals, and quasi drugs intended to improve wrinkles.

  All publications, patents and patent applications cited herein are incorporated herein by reference in their entirety.

Claims (4)

  A high-pressure treatment of the placenta at a pressure of 25 to 150 MPa to obtain a high-pressure treated product of the placenta; a step of separating the solid fraction from the high-pressure treated product of the placenta to separate and remove a solid fraction; A method for producing a high-pressure extraction placenta extract for wrinkle improvement, which does not include a placenta hydrolysis treatment step with enzyme addition, heat, acid, or alkali.   The manufacturing method according to claim 1, wherein the high-pressure treatment is performed within a range of 45 to 65 ° C.   The production method according to claim 1, wherein the liquid fraction is further subjected to at least one treatment selected from concentration, drying, and dilution.   The manufacturing method according to any one of claims 1 to 3, wherein the wrinkle improvement gives moisture to skin and makes wrinkles due to drying inconspicuous.

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