JP7222503B2 - Skin topical agent - Google Patents

Description

TECHNICAL FIELD The present invention relates to an external preparation for skin containing one or more selected from pine bark and yeast.

When living organisms use oxygen to acquire energy, part of the oxygen changes to active oxygen due to the influence of ultraviolet rays and the like. Active oxygen readily reacts with other substances, causes cross-linking of collagen fibers, fragmentation of hyaluronic acid, etc., and promotes skin aging such as increased wrinkles and reduced elasticity. In order to prevent such adverse effects of active oxygen on the living body, skin preparations for external use having antioxidant properties have been developed.

Examples of topical skin preparations having an antioxidant function include topical skin preparations containing a pine bark extract (Patent Document 1), and cosmetics containing supernatants collected from yeast cultures as antioxidants (Patent Document 2). , a cosmetic containing plant extracts such as hibiscus, aloe, rhubarb, yellow jade and urticaria as an antioxidant (Patent Document 3).

However, the external preparations for skin that have been developed so far may not exhibit their antioxidant function sufficiently when the content of the antioxidant is small. In addition, there were cases where the feeling of use was not favorable, and where the moisturizing effect was not sufficiently exhibited. Therefore, there has been a demand for the development of a new topical skin preparation having an antioxidant function.

JP-A-2003-238426 JP 2012-140618 A JP-A-06-024937

SUMMARY OF THE INVENTION Accordingly, an object of the present invention is to provide an external preparation for skin that exerts an antioxidant function, has a good feeling when used, and has a moisturizing effect.

As a result of intensive studies to solve the above problems, the present inventors have found that by combining one or more selected from pine bark and yeast with a specific ingredient, the antioxidant function is exhibited and the feeling of use is improved. The present inventors have found that an external preparation for skin with a good moisturizing effect can be obtained, which led to the completion of the present invention. The present invention has the following aspects.

[1] Selected from (A) one or more selected from pine bark and yeast, (B) one or more selected from placenta, hyaluronic acid and collagen, and (C) culture of aloe, royal jelly and apple fruit cells A skin external preparation comprising one or more.

[2] The external preparation for skin according to [1], wherein the component (A) is pine bark and the component (B) is placenta.

[3] The external preparation for skin according to [1], wherein the component (A) is yeast and the component (B) is hyaluronic acid.

[4] The external preparation for skin according to any one of [1] to [3], further comprising an ultraviolet scattering agent and/or an ultraviolet absorbing agent.

The external preparation for skin of the present invention exhibits an excellent antioxidant function by containing the components (A), (B) and (C). In addition, it is suitable for cosmetics because it gives a good feeling when used and has a moisturizing effect.

FIG. 4 shows the DPPH inhibitory activity of each test substance (Comparative Example and Examples 1 to 6). FIG. 4 shows the DPPH inhibitory activity of each test substance (Comparative Example and Examples 7 to 12).

The external preparation for skin of the present invention contains components (A), (B) and (C). Specifically, (A) one or more selected from pine bark and yeast, (B) one or more selected from placenta, hyaluronic acid and collagen, and (C) cultures of aloe, royal jelly and apple fruit cells One or more selected. Components (A) to (C) are described below.

(A) component (pine bark)
The pine that can be used in the present invention includes, for example, French maritime pine, larch, black pine, Japanese red pine, Japanese white pine, Japanese pine, Korean pine, Pumila pumila, Ryukyu pine, Utsukushima pine, Giant pine, White pine. of plants belonging to Among these, French maritime pine (scientific name: Pinus maritima) is preferable because of its excellent antioxidative effect, moisturizing effect, feeling of use, and the like.

As the pine bark used in the present invention, the above pine bark may be used as it is, but it is preferable to use a treated pine bark. The term "processed pine bark" as used in the present invention means a product obtained by subjecting the above pine bark to processing, and is a concept including fermented pine bark. Examples of processed pine bark products include pulverized pine bark obtained by pulverizing pine bark, pine bark extract obtained by extraction with water and/or organic solvent, and the like. These can be used either singly or in combination. Among these, it is preferable to use a pine bark extract from the viewpoint of effects and stability during use. In addition, the extract in the present invention is a concept including not only a liquid but also an extract dried by freeze-drying or the like, or a dried powder after adding an excipient or the like.

Examples of the extraction solvent used for obtaining the pine bark extract include water, organic solvents, and hydrous organic solvents (hydrous alcohol such as hydrous ethanol). Examples of organic solvents include methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, butane, acetone, hexane, cyclohexane, propylene glycol, hydrous ethanol, hydrous propylene glycol, ethyl methyl ketone, glycerin. , methyl acetate, ethyl acetate, diethyl ether, dichloromethane, edible fats and oils, 1,1,1,2-tetrafluoroethane, and 1,1,2-trichloroethene. These water and organic solvents may be used alone or in combination. Water is preferably used as the extraction solvent. In addition, the temperature of the solvent at the time of extraction is not limited as long as it is equal to or lower than the boiling point of the solvent used.

The method for obtaining the pine bark extract is not limited, but examples include heating extraction method, supercritical fluid extraction method, liquid carbon dioxide batch method, liquid carbon dioxide reflux method, supercritical carbon dioxide reflux method, and the like. be done. Moreover, you may combine several extraction methods. By combining multiple extraction methods, it is possible to obtain pine bark extracts with various compositions.

The supercritical fluid extraction method is a method of performing extraction using a supercritical fluid, which is a fluid in a state exceeding the critical point (critical temperature, critical pressure) of gas and liquid of a substance. As the supercritical fluid, carbon dioxide, ethylene, propane, nitrous oxide (laughing gas), etc. are used, and carbon dioxide is preferably used.

In the supercritical fluid extraction method, an extraction step of extracting a target component with a supercritical fluid and a separation step of separating the target component and the supercritical fluid are performed. In the separation step, any of extraction and separation by pressure change, extraction and separation by temperature change, and extraction and separation using an adsorbent/absorbent may be performed.

Alternatively, supercritical fluid extraction may be performed by an entrainer addition method. This method uses, for example, ethanol, propanol, n-hexane, acetone, toluene, other aliphatic lower alcohols, aliphatic hydrocarbons, aromatic hydrocarbons, and ketones in the extraction fluid at 2 to 20 W/V. % and performing supercritical fluid extraction using this fluid, the solubility of the desired extract such as OPC (oligomeric proanthocyanidin) and catechins in the extraction solvent is dramatically increased. or enhance the selectivity of separation, and is a method for obtaining an efficient pine bark extract.

Supercritical fluid extraction can be operated at relatively low temperatures, so it has the advantage of being applicable to substances that degrade or decompose at high temperatures. There is an advantage that the process can be omitted and the process becomes simple.

From the viewpoint of safety, it is preferable to purify the pine bark extract obtained by the above extraction by a column method or a batch method. The column method includes, for example, a purification method using an adsorptive carrier such as Diaion HP-20, Sephadex-LH20 and chitin.

As a commercially available pine bark extract, for example, flavangenol (manufactured by Toyo Shinyaku Co., Ltd., "Flavangenol" is a registered trademark of Toyo Shinyaku Co., Ltd.) can be used.

The amount of the pine bark of the present invention is not limited, but is preferably 0.0000001% by weight or more, more preferably 0.000001% by weight or more, and more preferably 0.00001% by weight or more, based on the total amount of the external skin preparation. More preferably, 0.0001% by weight or more is most preferable. The amount of pine bark is preferably 1% by weight or less, more preferably 0.8% by weight or less, still more preferably 0.5% by weight or less, and 0.3% by weight, based on the total amount of the external skin preparation. The following are more preferred, 0.2 wt% or less is even more preferred, and 0.1 wt% or less is most preferred.

(yeast)
The yeast that can be used in the present invention is not limited as long as it belongs to yeast.例えば、サッカロミセス セレビシエ（Ｓａｃｃｈａｒｏｍｙｃｅｓ ｃｅｒｅｖｉｓｉａｅ）、サッカロミセス アワモリ（Ｓａｃｃｈａｒｏｍｙｃｅｓ ａｗａｍｏｒｉ）等のサッカロミセス属の酵母、ジゴサッカロミセス ローキシ（Ｚｙｇｏｓａｃｃｈａｒｏｍｙｃｅｓ ｒｏｕｘｉｉ）、ジゴサッカロミセス ミソ（Ｚｙｇｏｓａｃｃｈａｒｏｍｙｃｅｓ ｍｉｓｏ）、ジゴサッカロミセス ラクティス（Ｚｙｇｏｓａｃｃｈａｒｏｍｙｃｅｓ ｌａｃｔｉｓ）等のジゴサッカロミセス属yeasts of the genus Candida such as Candida versatilis and Candida scottii, Aureobasidium Pullulans, Aureobasidium microstictum and the like and yeast of the genus. Among these, Saccharomyces cerevisiae is preferable because of its excellent antioxidant action, moisturizing effect, feeling of use, and the like.

As the yeast used in the present invention, the above yeast may be used as it is, but it is preferable to use a processed yeast. The yeast-processed product in the present invention means a product obtained by subjecting the above-described yeast to, for example, the following treatments. Processed yeast products include killed yeast processed products, yeast extracts, and the like. As the killed yeast, for example, edible yeast that has been killed by heat treatment or the like and then pulverized can be used. As the yeast extract, for example, a yeast decomposition product decomposed by autolysis, protease, acid hydrolysis, or the like may be used, or an extract of the obtained yeast decomposition product may be used. A yeast extract can be obtained, for example, by enzymatic hydrolysis of the yeast Saccharomyces cerevisiae and collection of the filtrate. A yeast extract is preferably used as the processed yeast product.

Although the amount of the yeast of the present invention is not limited, for example, it is preferably 0.0000001% by weight or more, more preferably 0.000001% by weight or more, and further preferably 0.00001% by weight or more, relative to the total amount of the external skin preparation. Preferably, 0.0001% by weight or more is most preferred. In addition, the yeast content is preferably 1% by weight or less, more preferably 0.8% by weight or less, even more preferably 0.5% by weight or less, and 0.3% by weight or less, relative to the total amount of the external preparation for skin. is more preferred, 0.2 wt% or less is even more preferred, and 0.1 wt% or less is most preferred.

(B) component (placenta)
The placenta in the present invention is a collective term for growth factors and other nutrients that promote cell division of the placenta of animals, preferably pigs, horses, cows, sheep, and humans, nutrients of the ovary membrane of fish, and nutritional components of plant germs. means As the placenta used in the present invention, the above placenta may be used as it is, but it is preferable to use a processed placenta. The placenta-treated product in the present invention means a product obtained by subjecting the above placenta to treatment, and is a concept including fermented placenta. A placenta extract is preferably used as the placenta-treated product of the present invention. Among placenta extracts, it is preferable to use an animal-derived placenta extract or a fish-derived placenta extract because of its excellent antioxidative action, moisturizing effect, feeling of use, etc., and the placenta extract derived from the fish ovary membrane is used. is most preferred.

The amount of the placenta of the present invention is not limited. Preferably, 0.0001% by weight or more is most preferred. The amount of placenta to be added is preferably 1% by weight or less, more preferably 0.8% by weight or less, even more preferably 0.5% by weight or less, and 0.3% by weight or less, relative to the total amount of the external preparation for skin. is more preferred, 0.2 wt% or less is even more preferred, and 0.1 wt% or less is most preferred.

(hyaluronic acid)
As the hyaluronic acid of the present invention, hyaluronic acid, acetylhyaluronic acid, derivatives of hyaluronic acid or acetylhyaluronic acid, or salts thereof may be used. may be used. Among these, it is preferable to use hydrolyzed hyaluronic acid because of its excellent antioxidative effect, moisturizing effect, feeling of use, and the like. The hydrolyzed hyaluronic acid in the present invention means low-molecular-weight hyaluronic acid having an average molecular weight of 10,000 or less. The hydrolyzed hyaluronic acid in the present invention is a concept including hydrolyzed hyaluronic acid or salts thereof.

The molecular weight of the hyaluronic acid used in the present invention varies depending on the number and type of repeating units, and is not limited. Hyaluronic acid having a weight average molecular weight of several hundred to several million may be used, or hydrolyzed hyaluronic acid having an average molecular weight of 10,000 or less may be used.

The method for producing hydrolyzed hyaluronic acid used in the present invention is not limited, but for example, after dissolving high-molecular-weight hyaluronic acid in a buffer solution, adding hyaluronidase and incubating for several days to deactivate the enzyme, a method of producing etc. Salts of hydrolyzed hyaluronic acid include, for example, sodium salts, potassium salts, basic amino acid salts and the like.

The amount of hyaluronic acid of the present invention is not limited. More preferably, 0.0001% by weight or more is most preferable. In addition, the amount of hyaluronic acid is preferably 1% by weight or less, more preferably 0.8% by weight or less, further preferably 0.5% by weight or less, and 0.3% by weight, based on the total amount of the external preparation for skin. The following are more preferred, 0.2 wt% or less is even more preferred, and 0.1 wt% or less is most preferred.

(collagen)
Collagen used in the present invention includes animal-derived collagen, fish-derived collagen, synthetic collagen, gelatin, collagen protein, collagen peptide obtained by degrading collagen protein, and collagen molecule treated with protease to remove the telopeptide portion. Atelocollagen or the like can be used. Among these, fish-derived collagen is preferable because it is excellent in antioxidative action, moisturizing effect, feeling of use, and the like. Fish-derived collagen is obtained by, for example, extracting gelatin (denatured collagen) from fish skins, bones, scales, etc. in hot or hot water, hydrolyzing it with acid, alkali, enzymes, etc., and pulverizing it. can be used.

The average molecular weight (weight average molecular weight) of the collagen of the present invention is not limited. The above is most preferable. The upper limit of the molecular weight is preferably 100,000 or less, more preferably 50,000 or less, even more preferably 30,000 or less, even more preferably 10,000 or less, even more preferably 8,000 or less, and most preferably 6,000 or less. In addition, collagen peptide is preferably used, and most preferably collagen peptide having an average molecular weight of 4,000 to 6,000 is used from the standpoint of excellent antioxidative action, moisturizing effect, feeling of use, and the like.

Although the amount of collagen of the present invention is not limited, for example, it is preferably 0.0000001% by weight or more, more preferably 0.000001% by weight or more, and further preferably 0.00001% by weight or more, relative to the total amount of the external skin preparation. Preferably, 0.0001% by weight or more is most preferred. In addition, the amount of collagen to be blended is preferably 1% by weight or less, more preferably 0.8% by weight or less, further preferably 0.5% by weight or less, and 0.3% by weight or less, relative to the total amount of the external preparation for skin. is more preferred, 0.2 wt% or less is even more preferred, and 0.1 wt% or less is most preferred.

(C) component (aloe)
Aloe that can be used in the present invention is not limited as long as it is a plant belonging to the genus Aloe. Examples include Aloe vera (Aloe barbadensis), Aloe arborescens Mill. var. natalensis Berg. Aloe plicatilis Mill., Aloe ferox Mill., Aloe perryi Baker, Aloe bainesii Th. Dyer., Aloe africana Mill., Aloescotrina (Aloe succotrina Lam.), Aloe spicata (Aloe spicata Baker) and the like. Among these, it is preferable to use Aloe vera (Aloe barbadensis) because of its excellent antioxidative action, moisturizing effect, feeling of use, and the like.

As the aloe used in the present invention, the above-mentioned aloe may be used as it is, but it is preferable to use a processed aloe. The term “processed aloe” in the present invention means a product obtained by processing the above aloe, and is a concept including fermented products of aloe. Examples of processed aloe products include aloe pulverized products and aloe extracts obtained by drying and pulverizing aloe. Among these, it is preferable to use an aloe extract.

The aloe extract in the present invention is a concept including not only an aloe extract obtained by extraction using water and/or an organic solvent, but also an aloe juice. As the aloe extract in the present invention, it is preferable to use an aloe juice product. In addition, the aloe squeezed product in the present invention is a concept including the product obtained by subjecting the aloe squeezed product to further processing such as extraction and purification.

Extraction solvents used in obtaining an aloe extract include, for example, water, organic solvents, and water-containing organic solvents (water-containing alcohols such as water-containing ethanol). Examples of organic solvents include methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, butane, acetone, hexane, cyclohexane, propylene glycol, hydrous ethanol, hydrous propylene glycol, ethyl methyl ketone, glycerin. , methyl acetate, ethyl acetate, diethyl ether, dichloromethane, edible fats and oils, 1,1,1,2-tetrafluoroethane, and 1,1,2-trichloroethene. These water and organic solvents may be used alone or in combination. Water is preferably used as the extraction solvent. In addition, the temperature of the solvent at the time of extraction is not limited as long as it is equal to or lower than the boiling point of the solvent used.

The part where aloe is used is not limited, and stems, leaves, flowers, and the like can be used. However, leaves are preferably used because of their excellent antioxidative effect, moisturizing effect, feeling of use, and the like.

Although the amount of the aloe vera of the present invention is not limited, it is preferably 0.0000001% by weight or more, more preferably 0.000001% by weight or more, and further preferably 0.00001% by weight or more, based on the total amount of the external skin preparation. Preferably, 0.0001% by weight or more is most preferred. In addition, the amount of aloe blended is preferably 1% by weight or less, more preferably 0.8% by weight or less, further preferably 0.5% by weight or less, and 0.3% by weight or less, relative to the total amount of the external skin preparation. is more preferred, 0.2 wt% or less is even more preferred, and 0.1 wt% or less is most preferred.

(royal jelly)
As the royal jelly used in the present invention, royal jelly may be used as it is, but it is preferable to use a royal jelly-treated product. The term "processed royal jelly" as used in the present invention means a product obtained by subjecting royal jelly to processing, and is a concept including fermented products of royal jelly. A fermented product of royal jelly is preferable as the processed product of royal jelly. A fermented royal jelly liquid is preferable as the fermented royal jelly product. A royal jelly fermented liquid refers to a liquid obtained by fermenting royal jelly with microorganisms such as lactic acid bacteria and yeast, followed by filtration. Although the type of microorganism to be fermented is not limited, it is preferable to use Lactobacillus because of its excellent antioxidative effect, moisturizing effect, feeling of use, and the like. As the royal jelly fermented liquid, a lactobacillus/royal jelly fermented liquid is preferable.

Although the amount of the royal jelly of the present invention is not limited, it is preferably 0.0000001% by weight or more, more preferably 0.000001% by weight or more, and further preferably 0.00001% by weight or more, based on the total amount of the external preparation for skin. Preferably, 0.0001% by weight or more is most preferred. The amount of royal jelly to be blended is preferably 1% by weight or less, more preferably 0.8% by weight or less, even more preferably 0.5% by weight or less, and 0.3% by weight or less, relative to the total amount of the external skin preparation. is more preferred, 0.2 wt% or less is even more preferred, and 0.1 wt% or less is most preferred.

(Culture of apple fruit cells)
In the present invention, a culture of apple fruit cells refers to a culture of fruit cells of apple (Malus domestica) or a processed culture thereof. Among these, the extract of apple fruit cell culture is preferred. As the extract of apple fruit cell culture, for example, plant stem cells obtained from the fruit of Uttwiler Spatlauber, which is a kind of Western apple, are artificially cultured, and an extract is obtained from the obtained cells. It can be obtained by extraction. As the apple fruit cell culture extract, an apple fruit culture cell extract is preferred.

The amount of the apple fruit cell culture extract of the present invention is not limited. More preferably 0.00001% by weight or more, most preferably 0.0001% by weight or more. In addition, the amount of the culture of apple fruit cells is preferably 1% by weight or less, more preferably 0.8% by weight or less, further preferably 0.5% by weight or less, with respect to the total amount of the external skin preparation. 0.3 wt % or less is more preferred, 0.2 wt % or less is even more preferred, and 0.1 wt % or less is most preferred.

(Ultraviolet scattering agent/ultraviolet absorber)
The external preparation for skin of the present invention can enhance the effects of the ultraviolet scattering agent and the ultraviolet absorber. Therefore, the external preparation for skin of the present invention preferably contains an ultraviolet scattering agent and/or an ultraviolet absorber.

Examples of UV scattering agents that can be used in the present invention include, but are not limited to, metal oxides such as zinc oxide, titanium oxide, cerium oxide, low order titanium oxide, and iron-doped titanium oxide. Among these, zinc oxide is preferred.

Although the amount of the ultraviolet scattering agent of the present invention is not limited, it is preferably 0.1% by weight or more, more preferably 1% by weight or more, and further preferably 3% by weight or more, relative to the total amount of the external skin preparation. More than 5% by weight is most preferred. The amount of the UV scattering agent is preferably 50% by weight or less, more preferably 40% by weight or less, still more preferably 30% by weight or less, and even more preferably 20% by weight or less, relative to the total amount of the external skin preparation. 18 wt% or less is even more preferred, and 15 wt% or less is most preferred.

Examples of ultraviolet absorbers that can be used in the present invention include, but are not limited to, ethylhexyl methoxycinnamate, hexyl diethylaminohydroxybenzoylbenzoate, benzotriazole, t-butylmethoxydibenzoylmethane, and the like. Among these, ethylhexyl methoxycinnamate and/or hexyl diethylaminohydroxybenzoylbenzoate are preferred.

Although the amount of the ultraviolet absorber of the present invention is not limited, for example, it is preferably 0.1% by weight or more, more preferably 1% by weight or more, and further preferably 3% by weight or more, relative to the total amount of the external skin preparation. 4% by weight or more is most preferred. In addition, the amount of the ultraviolet absorber is preferably 30% by weight or less, more preferably 20% by weight or less, still more preferably 25% by weight or less, and even more preferably 20% by weight or less, relative to the total amount of the external skin preparation. 18 wt% or less is even more preferred, and 15 wt% or less is most preferred.

(thickener)
The external preparation for skin of the present invention can be improved in usability and stability by blending a thickener. Therefore, the external preparation for skin of the present invention preferably contains a thickening agent.

Thickeners that can be used in the present invention include, but are not limited to, xanthan gum, hydroxyethyl cellulose, carboxymethyl cellulose, and acrylic polymers. Among these, it is preferable to use an acrylic polymer from the viewpoint of improving the feeling of use and water resistance. Among acrylic polymers, it is preferable to use a (dimethyltaurate ammonium acrylate/vinylpyrrolidone) copolymer.

Although the amount of the thickener of the present invention is not limited, it is preferably 0.01% by weight or more, more preferably 0.03% by weight or more, and 0.05% by weight or more, based on the total amount of the external skin preparation. is more preferred, and 0.1% by weight or more is most preferred. The amount of the thickening agent is preferably 5% by weight or less, more preferably 3% by weight or less, even more preferably 2% by weight or less, and even more preferably 1% by weight or less, relative to the total amount of the external preparation for skin. 0.8 wt% or less is even more preferred, and 0.5 wt% or less is most preferred.

(optional component)
The external preparation for skin of the present invention can contain optional ingredients used in external preparation for skin, in addition to the above ingredients. Examples of such components include oils and fats, waxes, alcohols, esters, preservatives, surfactants, chelating agents, pH adjusters, stabilizers, extracts extracted from plants or animals, and the like. .

(Composition ratio of ingredients)
In the external preparation for skin of the present invention, the mixing ratio of component (B) to component (A) is not limited, but the lower limit of the amount of component (B) to 1 part by weight of component (A) is, for example, 0.001. Part by weight or more is preferable, 0.01 part by weight or more is more preferable, 0.05 part by weight or more is still more preferable, 0.1 part by weight or more is even more preferable, 0.5 part by weight or more is even more preferable, and 0.9 Parts by weight or more are most preferred. Further, the upper limit of the amount of component (B) with respect to 1 part by weight of component (A) is, for example, preferably 100 parts by weight or less, more preferably 50 parts by weight or less, further preferably 15 parts by weight or less, and 10 parts by weight. The following is more preferred, 8 parts by weight or less is even more preferred, and 5 parts by weight or less is most preferred.

In the external preparation for skin of the present invention, the mixing ratio of component (C) to component (A) is not limited, but the lower limit of the amount of component (C) to 1 part by weight of component (A) is, for example, 0.0001. Part by weight or more is preferable, 0.001 part by weight or more is more preferable, 0.002 part by weight or more is still more preferable, 0.003 part by weight or more is still more preferable, 0.005 part by weight or more is even more preferable, and 0.01 Parts by weight or more are most preferred. Further, the upper limit of the amount of component (B) with respect to 1 part by weight of component (A) is, for example, preferably 100 parts by weight or less, more preferably 40 parts by weight or less, further preferably 30 parts by weight or less, and 10 parts by weight. The following is more preferred, 2 parts by weight or less is even more preferred, and 1 part by weight or less is most preferred.

(External preparation for skin of the present invention)

Formulations of the external preparation for skin of the present invention include water-based, oil-based, emulsified (water-in-oil, oil-in-water, etc.), solubilized, powder-based and solvent-based formulations. Among these, an oil-in-water emulsion is preferable.

The external preparation for skin of the present invention can be used for any of cosmetics, quasi-drugs, and pharmaceuticals. Since the external preparation for skin of the present invention has antioxidative and moisturizing effects and a good feel when used, it is preferably used as a cosmetic. Note that the term "cosmetics" as used herein refers to a concept that includes cosmetics classified as quasi-drugs in addition to cosmetics.

Cosmetics of the present invention include, for example, BB creams, serums, milky lotions, makeup bases, foundations, packs, cleansing agents, and sunscreen cosmetics. Among these, sunscreen cosmetics are preferable. In addition, the sunscreen cosmetic in the present invention is a concept including cosmetics having a sunscreen function by blending an ultraviolet absorber and/or an ultraviolet scattering agent. Specifically, sunscreen cosmetics also include BB creams, lip creams, and the like with sunscreen functions.

Since the external preparation for skin of the present invention has an antioxidant function, it can be used as an antioxidant cosmetic or an anti-aging cosmetic. In addition, since the external preparation for skin of the present invention has an excellent moisturizing effect, it can be used as a moisturizing cosmetic.

Since the external skin preparation of the present invention has an effect of improving skin elasticity, it can be used as a composition for improving skin firmness, a composition for improving wrinkles or sagging, a composition for maintaining skin elasticity, a cosmetic composition, and as an anti-aging composition.

EXAMPLES The present invention will be described below with reference to Examples, but the present invention is not limited to these Examples and can take various forms.

[Test 1: Evaluation of antioxidant effect]
The DPPH radical scavenging activity was measured by the method described below to evaluate the antioxidant activity.

(Preparation of test substance)
Preparation of pine bark extract Pine bark extract (“Flavangenol (registered trademark)” manufactured by Toyo Shinyaku Co., Ltd.) was diluted with purified water so that the concentration was 0.0025 mg/mL, and used for DPPH activity measurement. was used as the test substance.

Preparation of Apple Fruit Cultured Cell Extract An apple fruit cultured cell extract “PhytoCellTec Md or pf (PCT Apple Extract PF)” (manufactured by Arista Health and Nutrition Science) was suspended in purified water. After that, centrifugation (10,000 rpm, 1 minute) was performed and the supernatant was collected. The resulting supernatant was diluted with purified water to 2.5 mg/mL, and used as a test substance for measuring DPPH activity.

Preparation of other test substances Yeast, placenta, collagen, hyaluronic acid, aloe and royal jelly were each diluted with purified water to a concentration of 2.5 mg/mL and used as test substances for measuring DPPH activity. . The raw materials used are as follows.
Yeast: Saccharomyces lysate extract Placenta: Placenta extract derived from salmon ovary membrane Collagen: Collagen peptide (average molecular weight about 5000, derived from scales of Threadworm)
Hyaluronic Acid: Sodium Hyaluronate, Hydrolyzed Hyaluronic Acid Aloe: Aloe Vera Leaf Extract (Aloe Vera Juice)
Royal jelly: Lactobacillus/royal jelly fermented liquid

(Measurement of DPPH radical scavenging activity)
The prepared test substance for DPPH activity measurement was added to a 96-well plate in the amounts shown in Tables 1 and 2. In Control, purified water was added instead of the test substance.

After 80 μL of 100% ethanol was added to the blank wells, 80 μL of 0.25 mM DPPH was added to the test wells, allowed to stand at room temperature for 20 minutes, and the absorbance at 516 nm was measured with VARIOSKAN. A DPPH inhibitory activity value (inhibition rate %) was calculated from Equation 1 from the obtained absorbance.
(Formula 1)

(Test results)
The test results are shown in FIGS. 1 and 2. A positive value means that the DPPH inhibitory activity is higher than that of the control, and a negative value means that the DPPH inhibitory activity is lower than that of the control. Almost no DPPH inhibitory activity was observed when components (A) to (C) were added alone. On the other hand, when the components (A), (B) and (C) were added in combination, high DPPH inhibitory activity was observed. In Comparative Example 1, DPPH inhibitory activity was observed even with the component (A) alone, but it was confirmed that the inhibitory activity was further enhanced by combining the components (B) and (C). From the above, it was found that a synergistic effect is exhibited by combining the (A) component, the (B) component and the (C) component, and the anti-oxidation effect is remarkably improved. Therefore, the compositions of the present invention can be used as antioxidants.

[Test 2: Evaluation of effect by single use of cosmetics]
The effects of single use of cosmetics were evaluated by the method described below.

(Preparation of sunscreen cosmetics)
A sunscreen cosmetic was prepared according to the formulation shown in Table 3.

(Evaluation of effect by single use)
For 3 expert panelists, apply each cosmetic in an amount of about 2 grains of rice to the skin on the inside of the forearm (4 cm x 4 cm square), measure the moisture content of the stratum corneum, transepidermal water loss and elasticity, and The usability was evaluated by a questionnaire.

1) Evaluation of feeling in use Each evaluation item (spreadability, stickiness, moist feeling, elasticity, smoothness, smooth feeling) was evaluated. The evaluation was made on a 7-point scale, with "1" being "extremely bad", "4" being "normal", and "7" being "extremely good".

Table 4 shows the evaluation results of the feeling of use (numerical values are average values). Compared to Comparative Examples 10 and 11 in which only component (A) was added, Comparative Example 12 in which only component (B) was added, and Comparative Example 13 in which only component (C) was added, (A) component, (B) In Examples 13 to 17 in which the component and the component (C) were added in combination, high evaluations were obtained for all items. From this, it was found that a synergistic effect is exhibited by combining the (A) component, the (B) component and the (C) component, and the feeling of use is remarkably improved. In addition, Example 16 containing yeast extract, apple fruit culture cell extract and hydrolyzed hyaluronic acid was highly evaluated in most items compared to Example 15 in which sodium hyaluronate was blended instead of hydrolyzed hyaluronic acid. From this, it was found that when hydrolyzed hyaluronic acid was selected as the component (B), the feeling of use was particularly good.

2) Measurement of stratum corneum water content, water loss and elasticity Three women in their twenties and thirties were used as subjects and the stratum corneum water content, water loss and elasticity were measured. After 15 minutes of acclimatization (at a room temperature of 20 to 25° C.), the stratum corneum water content, water loss and elasticity before application were measured. After that, each sunscreen cosmetic was applied in an amount of about 2 grains of rice to the skin on the inside of the forearm (4 cm x 4 cm), and measurements were taken 15 minutes, 30 minutes, 1 hour, and 3 hours after application.

The stratum corneum moisture content was measured using SKICON-200EX (manufactured by Yayoi Co., Ltd.). The amount of water loss was measured using a Tevameter (registered trademark) TM300 (manufactured by Courage+Khazaka). Elasticity was measured using a skin viscoelasticity measuring device Cutometer MPA580 (manufactured by Courage+Khazaka).

Table 5 shows the rate of change in the stratum corneum moisture content before and after application (the value before application is set to 1, and the value after application is shown as a relative value to before application). Compared to Comparative Examples 10 and 11 in which only component (A) was added, Comparative Example 12 in which only component (B) was added, and Comparative Example 13 in which only component (C) was added, (A) component, (B) In Examples 13 to 17, in which the component and the component (C) were added in combination, the stratum corneum water content was greatly increased. From this, it was found that a synergistic effect is exhibited by combining the components (A), (B) and (C), and the water content of the stratum corneum is remarkably increased. Therefore, the composition of the present invention is effective as a moisturizing agent and can be used as an agent for increasing the water content of the stratum corneum.

Table 6 shows the rate of change in the amount of water transpiration before and after coating (the value before coating is set to 1, and the value after coating is shown as a relative value to before coating). Compared to Comparative Examples 10 and 11 in which only component (A) was added, Comparative Example 12 in which only component (B) was added, and Comparative Example 13 in which only component (C) was added, (A) component, (B) In Examples 13 to 17 in which the component and the component (C) were added in combination, the amount of water loss was greatly suppressed. From this, it was found that a synergistic effect is exhibited by combining the (A) component, the (B) component and the (C) component, and the water loss amount is remarkably suppressed. Therefore, the composition of the present invention is effective as a moisturizing agent and can be used as an inhibitor of water loss.

The rate of change in elasticity before and after application is shown in Table 7 (the value before application is set to 1, and the value after application is shown as a relative value to before application). Compared to Comparative Examples 10 and 11 in which only component (A) was added, Comparative Example 12 in which only component (B) was added, and Comparative Example 13 in which only component (C) was added, (A) component, (B) In Examples 13 to 17, in which the component and the component (C) were added in combination, skin elasticity was greatly increased. From this, it was found that the combination of components (A), (B) and (C) exerts a synergistic effect and remarkably improves skin elasticity. Therefore, the composition of the present invention can be used as a skin elasticity improving agent.

[Test 3: Evaluation of effects of continuous use of cosmetics]
Using the above sunscreen cosmetic prepared in Test 2, the effects of continuous use of the cosmetic were evaluated. Specifically, a clinical test was conducted with two women in their twenties and thirties as subjects. The subjects applied each sunscreen cosmetic in an amount of about two grains of rice to the outside of the forearm (4 cm x 4 cm) every morning for two weeks. Before and after the application period, the L value was measured using a color difference meter (CR-400 manufactured by Konica Minolta).

Table 8 shows the rate of change in the L value before and after coating (the value before coating is set to 1, and the value after coating is shown as a relative value to before coating). Note that the L value is a numerical value that is an index of the brightness of the skin, and the higher the L value, the less sunburned. Compared to Comparative Examples 10 and 11 in which only component (A) was added, Comparative Example 12 in which only component (B) was added, and Comparative Example 13 in which only component (C) was added, (A) component, (B) In Examples 13 to 17 in which the component and the component (C) were added in combination, the L value increased significantly. From this, it was found that a synergistic effect is exhibited by combining the (A) component, the (B) component and the (C) component, and a remarkable sunscreen effect is exhibited. Therefore, the composition of the present invention can be used as a sunscreen cosmetic.

The external preparation for skin of the present invention has an antioxidant function, is excellent in feeling when used, and has a high moisturizing effect, and therefore can be used as cosmetics, quasi-drugs, and pharmaceuticals. In addition, since the external preparation for skin of the present invention has an excellent sunscreen effect, it can be used as a sunscreen cosmetic.

Claims (2)

(A) pine bark extract, (B) placenta, and (C) one or more selected from aloe extract and royal jelly fermentation liquid ,
Furthermore, an external skin preparation (French maritime pine bark extract, soluble collagen, hydrolyzed collagen, sodium hyaluronate crosspolymer, placenta extract, apple fruit A skin care cosmetic containing a cell culture, Novara oil, Mg ascorbyl phosphate, cyanocobalamin, sodium citrate, potassium hydroxide, melissa leaf extract, chamomile flower extract and pueraria mirifica root extract, and
French Maritime Pine Bark Extract, Placenta Extract, Aloe Leaf Extract, Royal Jelly Extract, Pueraria Mirifica Root Extract, Chrysanthemum Indicum Extract, Rooibos Extract, Artemisia Leaf Extract, Gennoshoko Flower/Leaf/Stem Extract, Scutellaria root Extract, Angelica keiskei Leaf /Stem Extract, Olive Leaf Extract, Bilberry Leaf Extract, Alpinia Leaf Extract, Rockberry Root Extract, Vervain Extract, Evening Primrose Seed Extract, Mallow Flower/Leaf/Stem Extract, Mint Leaf Extract, Primrose Extract, Rhododendron Extract, Veronica Officinalis Skin care cosmetics containing flower/leaf/stem extract, melissa leaf extract, yarrow extract, kudzu root extract, chlorella extract, peony root extract, saxifrage extract, palmarosa oil, fennel fruit oil, coriander fruit oil, and odorous mango oil ,except for). 2. The external preparation for skin according to claim 1, which is a sunscreen cosmetic.

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