CN110292554A - A kind of cosmetics and preparation method thereof containing placenta tissue extract - Google Patents
A kind of cosmetics and preparation method thereof containing placenta tissue extract Download PDFInfo
- Publication number
- CN110292554A CN110292554A CN201910521288.XA CN201910521288A CN110292554A CN 110292554 A CN110292554 A CN 110292554A CN 201910521288 A CN201910521288 A CN 201910521288A CN 110292554 A CN110292554 A CN 110292554A
- Authority
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- weight
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- tissue extract
- placenta tissue
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- 210000005059 placental tissue Anatomy 0.000 title claims abstract description 75
- 239000002537 cosmetic Substances 0.000 title claims abstract description 67
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 239000000203 mixture Substances 0.000 claims abstract description 40
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims abstract description 26
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims abstract description 26
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims abstract description 26
- 229930194248 Licoflavone Natural products 0.000 claims abstract description 23
- MEHHCBRCXIDGKZ-UHFFFAOYSA-N Licoflavone C Natural products CC(C)=CCC1=C(O)C=C(O)C(C(C=2)=O)=C1OC=2C1=CC=C(O)C=C1 MEHHCBRCXIDGKZ-UHFFFAOYSA-N 0.000 claims abstract description 23
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 42
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 claims description 28
- POJWUDADGALRAB-UHFFFAOYSA-N allantoin Chemical compound NC(=O)NC1NC(=O)NC1=O POJWUDADGALRAB-UHFFFAOYSA-N 0.000 claims description 26
- 210000002826 placenta Anatomy 0.000 claims description 21
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 20
- 238000000034 method Methods 0.000 claims description 18
- -1 propylben Chemical compound 0.000 claims description 17
- 229920000663 Hydroxyethyl cellulose Polymers 0.000 claims description 15
- 239000004354 Hydroxyethyl cellulose Substances 0.000 claims description 14
- 235000013871 bee wax Nutrition 0.000 claims description 14
- 239000012166 beeswax Substances 0.000 claims description 14
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 14
- 235000019447 hydroxyethyl cellulose Nutrition 0.000 claims description 14
- 235000010270 methyl p-hydroxybenzoate Nutrition 0.000 claims description 14
- 239000004292 methyl p-hydroxybenzoate Substances 0.000 claims description 14
- 229960002216 methylparaben Drugs 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- JVTIXNMXDLQEJE-UHFFFAOYSA-N 2-decanoyloxypropyl decanoate 2-octanoyloxypropyl octanoate Chemical compound C(CCCCCCC)(=O)OCC(C)OC(CCCCCCC)=O.C(=O)(CCCCCCCCC)OCC(C)OC(=O)CCCCCCCCC JVTIXNMXDLQEJE-UHFFFAOYSA-N 0.000 claims description 13
- POJWUDADGALRAB-PVQJCKRUSA-N Allantoin Natural products NC(=O)N[C@@H]1NC(=O)NC1=O POJWUDADGALRAB-PVQJCKRUSA-N 0.000 claims description 13
- 229960000458 allantoin Drugs 0.000 claims description 13
- 229940082500 cetostearyl alcohol Drugs 0.000 claims description 13
- XUGNVMKQXJXZCD-UHFFFAOYSA-N isopropyl palmitate Chemical compound CCCCCCCCCCCCCCCC(=O)OC(C)C XUGNVMKQXJXZCD-UHFFFAOYSA-N 0.000 claims description 13
- GLDOVTGHNKAZLK-UHFFFAOYSA-N octadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCCCO GLDOVTGHNKAZLK-UHFFFAOYSA-N 0.000 claims description 13
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 claims description 13
- OULAJFUGPPVRBK-UHFFFAOYSA-N tetratriacontyl alcohol Natural products CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCO OULAJFUGPPVRBK-UHFFFAOYSA-N 0.000 claims description 13
- QCDWFXQBSFUVSP-UHFFFAOYSA-N 2-phenoxyethanol Chemical compound OCCOC1=CC=CC=C1 QCDWFXQBSFUVSP-UHFFFAOYSA-N 0.000 claims description 12
- HIQIXEFWDLTDED-UHFFFAOYSA-N 4-hydroxy-1-piperidin-4-ylpyrrolidin-2-one Chemical compound O=C1CC(O)CN1C1CCNCC1 HIQIXEFWDLTDED-UHFFFAOYSA-N 0.000 claims description 12
- 229920001214 Polysorbate 60 Polymers 0.000 claims description 12
- 229940119170 jojoba wax Drugs 0.000 claims description 12
- 229960005323 phenoxyethanol Drugs 0.000 claims description 12
- 239000006228 supernatant Substances 0.000 claims description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 10
- 239000012153 distilled water Substances 0.000 claims description 10
- 239000000243 solution Substances 0.000 claims description 10
- 235000019441 ethanol Nutrition 0.000 claims description 8
- 239000006166 lysate Substances 0.000 claims description 8
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims description 7
- 238000011010 flushing procedure Methods 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 7
- 230000008569 process Effects 0.000 claims description 7
- 229960005150 glycerol Drugs 0.000 claims description 6
- 229940075495 isopropyl palmitate Drugs 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 5
- 239000003814 drug Substances 0.000 claims description 5
- 229910052757 nitrogen Inorganic materials 0.000 claims description 5
- 239000002504 physiological saline solution Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 4
- 229940074410 trehalose Drugs 0.000 claims description 4
- 229940079593 drug Drugs 0.000 claims description 3
- 150000002576 ketones Chemical class 0.000 claims description 3
- 238000010257 thawing Methods 0.000 claims description 3
- 241000195493 Cryptophyta Species 0.000 claims description 2
- 150000001412 amines Chemical class 0.000 claims description 2
- 229940071826 hydroxyethyl cellulose Drugs 0.000 claims description 2
- 239000008194 pharmaceutical composition Substances 0.000 claims description 2
- YAVVGPBYBUYPSR-UHFFFAOYSA-N benzene;oxygen Chemical compound [O].C1=CC=CC=C1 YAVVGPBYBUYPSR-UHFFFAOYSA-N 0.000 claims 1
- ZZVUWRFHKOJYTH-UHFFFAOYSA-N diphenhydramine Chemical compound C=1C=CC=CC=1C(OCCN(C)C)C1=CC=CC=C1 ZZVUWRFHKOJYTH-UHFFFAOYSA-N 0.000 claims 1
- 125000004079 stearyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 claims 1
- 230000002087 whitening effect Effects 0.000 abstract description 22
- 230000003020 moisturizing effect Effects 0.000 abstract description 11
- 239000004615 ingredient Substances 0.000 abstract description 9
- 238000012360 testing method Methods 0.000 description 35
- 230000000694 effects Effects 0.000 description 30
- 230000001153 anti-wrinkle effect Effects 0.000 description 12
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 210000003491 skin Anatomy 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 230000002354 daily effect Effects 0.000 description 3
- 230000002708 enhancing effect Effects 0.000 description 3
- 229940031098 ethanolamine Drugs 0.000 description 3
- 230000003203 everyday effect Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 description 2
- 206010040829 Skin discolouration Diseases 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 238000004500 asepsis Methods 0.000 description 2
- 230000003796 beauty Effects 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000005360 mashing Methods 0.000 description 2
- 239000000155 melt Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000012982 microporous membrane Substances 0.000 description 2
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 210000003954 umbilical cord Anatomy 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 102400000739 Corticotropin Human genes 0.000 description 1
- 101800000414 Corticotropin Proteins 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 201000004624 Dermatitis Diseases 0.000 description 1
- AXISYYRBXTVTFY-UHFFFAOYSA-N Isopropyl tetradecanoate Chemical compound CCCCCCCCCCCCCC(=O)OC(C)C AXISYYRBXTVTFY-UHFFFAOYSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 241000221095 Simmondsia Species 0.000 description 1
- 235000004433 Simmondsia californica Nutrition 0.000 description 1
- 102000009618 Transforming Growth Factors Human genes 0.000 description 1
- 108010009583 Transforming Growth Factors Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229960000258 corticotropin Drugs 0.000 description 1
- IDLFZVILOHSSID-OVLDLUHVSA-N corticotropin Chemical compound C([C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(N)=O)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)NC(=O)[C@@H](N)CO)C1=CC=C(O)C=C1 IDLFZVILOHSSID-OVLDLUHVSA-N 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 210000002219 extraembryonic membrane Anatomy 0.000 description 1
- 230000001815 facial effect Effects 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 239000003163 gonadal steroid hormone Substances 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 210000003780 hair follicle Anatomy 0.000 description 1
- 230000003054 hormonal effect Effects 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 201000001881 impotence Diseases 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000000186 progesterone Substances 0.000 description 1
- 229960003387 progesterone Drugs 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000000751 protein extraction Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
- A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7016—Disaccharides, e.g. lactose, lactulose
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/50—Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
- A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/731—Cellulose; Quaternized cellulose derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/81—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions involving only carbon-to-carbon unsaturated bonds
- A61K8/8141—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides or nitriles thereof; Compositions of derivatives of such polymers
- A61K8/8147—Homopolymers or copolymers of acids; Metal or ammonium salts thereof, e.g. crotonic acid, (meth)acrylic acid; Compositions of derivatives of such polymers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
- A61K8/982—Reproductive organs; Embryos, Eggs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Dermatology (AREA)
- Developmental Biology & Embryology (AREA)
- Reproductive Health (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Pregnancy & Childbirth (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Cosmetics (AREA)
Abstract
The present invention provides a kind of cosmetics and preparation method thereof containing placenta tissue extract, specific cosmetic composition provided by the invention contains placenta tissue extract, licoflavone, trehalose and other functional ingredient, significant synergy is shown between functional component, is obviously improved at moisturizing, whitening and crease-resistant aspect.
Description
Technical field
The invention belongs to cosmetic fields, and in particular to a kind of cosmetics containing placenta tissue extract and its preparation side
Method.
Background technique
Human plactnta is also known as afterbirth, afterbirth, afterbirth, fetal membrane.The entitled dried human placenta of placenta Chinese medicine has and adjusts function of human body, increases
Strong resistance, the effect for the essence and blood that reinforces vital energy.Modern medicine study shows that placenta contains protein, sugar, calcium, vitamin, is immunized
The factor, female sex hormone, promoting sexual gland hormone, corticotropin etc. can be used for consumptive disease, hectic fever due to yin, cough, deficiency of food, impotence, no
It is pregnant etc..Presently, there are the problems such as source difficulty, drug safety.
Pig placenta is same as Human plactnta to derive from mammal, natural component rich in, eutrophy and has
Multiple biological activities.But, serious waste of resources lower currently with rate.
As the improvement of people's living standards, the demand for cosmetics is more and more vigorous, and therefore, those skilled in the art
It is dedicated to developing the better cosmetics of resultant effect, to meet the demands such as the whitening of people, moisturizing, crease-resistant.
Summary of the invention
The purpose of the present invention is to provide a kind of cosmetics and preparation method thereof containing placenta tissue extract.
In the first aspect of the present invention, a kind of composition is provided, the composition includes placenta tissue extract and sweet
Straw colour ketone.
In another preferred example, the composition is cosmetic composition or pharmaceutical composition.
In another preferred example, the composition further includes trehalose.
In another preferred example, include: in the composition
Placenta tissue extract 0.1-5 parts by weight；With
Licoflavone 0.1-2 parts by weight.
In another preferred example, include: in the composition
Placenta tissue extract 0.1-5 parts by weight；
Licoflavone 0.1-1 parts by weight；With
Trehalose 1-10 parts by weight.
In another preferred example, include: in the composition
Placenta tissue extract 2-5 parts by weight.
In another preferred example, include: in the composition
Licoflavone 0.2-0.5 parts by weight.
In another preferred example, include: in the composition
Trehalose 2-6 parts by weight.
In another preferred example, the composition further includes one or more components selected from the group below:
Citric acid monoglyceride, propylben, Phenoxyethanol, jojoba oil, isopropyl palmitate, is spat at methylparaben
Warm -60, cetostearyl alcohol, beeswax, atoleine, Carbopol 941, glycerol, allantoin, hydroxyethyl cellulose, EDETATE SODIUM, three
Ethanol amine, isopropyl myristate and essence.
In another preferred example, the composition includes:
Citric acid monoglyceride 1-5 parts by weight, methylparaben 0.1-1 parts by weight, propylben 0.1-1 parts by weight, benzene
Oxyethanol 0.1-1 parts by weight, jojoba oil 1-10 parts by weight, isopropyl palmitate 1-10 parts by weight, Tween-60 1-5 weight
Part, cetostearyl alcohol 1-5 parts by weight, beeswax 1-5 parts by weight, atoleine 1-5 parts by weight, Carbopol 941 0.1-0.5 weight
Part, trehalose 1-10 parts by weight, glycerol 1-10 parts by weight, allantoin 0.1-0.5 parts by weight, hydroxyethyl cellulose 0.1-1 weight
It is part, EDETATE SODIUM 0.01-0.5 parts by weight, triethanolamine 0.01-0.5 parts by weight, isopropyl myristate 1-10 parts by weight, sweet
Straw colour ketone 0.1-1 parts by weight, essence 0.01-0.1 parts by weight, placenta tissue extract 0.1-5 parts by weight.
In another preferred example, the placenta tissue extract of the placenta tissue extract behaviour or pig；Preferably, described
Placenta tissue extract is the placenta tissue extract of pig.
In another preferred example, the placenta tissue extract preparation method comprising steps of
(1) it pre-processes
Clean placenta is put into ethanol solution and is impregnated 1~3 hour, normal saline flushing is then used；
(2) it smashs to pieces
It is smashed to pieces after pretreated placenta is mixed with physiological saline, obtains stamping；
(3) freeze thawing
Stamping is cracked using liquid nitrogen multigelation 2~5 times, obtained lysate；
(4) it is centrifuged
Lysate is centrifuged, supernatant is collected；
(5) it keeps the temperature
Supernatant is placed under 50~80 DEG C of environment 1~5 hour, the placenta tissue extract is obtained.
In another preferred example, the method also includes steps:
(6) degerming
Bacteria removing is carried out to the placenta tissue extract of acquisition；
Preferably, bacteria removing is carried out by the way of filtering with microporous membrane.
In another preferred example, the placenta tissue extract is liquid, also, albumen in the placenta tissue extract
Concentration is 0.2-0.5mg/ml；Preferably 0.25-0.35mg/ml.
The second aspect of the present invention provides a kind of preparation method of cosmetics, comprising steps of
(I) by citric acid monoglyceride, methylparaben, propylben, Phenoxyethanol, jojoba oil, palmitinic acid isopropyl
Ester, Tween-60, cetostearyl alcohol, beeswax and atoleine are heated to 65~75 DEG C, mix, obtain component A；
(II) hydroxyethyl cellulose and distilled water are mixed, is heated to 80~90 DEG C, stirring is transparent to solution, is cooled to
40 DEG C hereinafter, add Carbopol 941, trehalose, glycerol, allantoin, EDETATE SODIUM, placenta tissue extract again, mix, obtain
Component B；
(III) component A is cooled to 40 DEG C hereinafter, component B is then added to component A, and sequentially add three under stiring
Ethanol amine, isopropyl myristate, licoflavone and essence.
In another preferred example, the placenta tissue extract preparation method comprising steps of
(1) it pre-processes
Clean placenta is put into ethanol solution and is impregnated 1~3 hour, normal saline flushing is then used；
(2) it smashs to pieces
It is smashed to pieces after pretreated placenta is mixed with physiological saline, obtains stamping；
(3) freeze thawing
Stamping is cracked using liquid nitrogen multigelation 2~5 times, obtained lysate；
(4) it is centrifuged
Lysate is centrifuged, supernatant is collected；
(5) it keeps the temperature
Supernatant is placed under 50~80 DEG C of environment 1~5 hour, the placenta tissue extract is obtained.
In another preferred example, the method also includes steps:
(6) degerming
Bacteria removing is carried out to the placenta tissue extract of acquisition；
Preferably, bacteria removing is carried out by the way of filtering with microporous membrane.
In another preferred example, the placenta tissue extract is liquid, also, albumen in the placenta tissue extract
Concentration is 0.2-0.5mg/ml；Preferably 0.25-0.35mg/ml.
In another preferred example, 70 DEG C are heated in the step (I).
In another preferred example, 85 DEG C are heated in the step (II).
In another preferred example, 40 DEG C are cooled in the step (II).
The third aspect of the present invention provides composition described in first aspect present invention in preparing cosmetics or drug
Application.
In another preferred example, the cosmetics are moisturizing cosmetic, skin-lightening cosmetic, and/or anti-wrinkle cosmetic.
It should be understood that above-mentioned each technical characteristic of the invention and having in below (eg embodiment) within the scope of the present invention
It can be combined with each other between each technical characteristic of body description, to form a new or preferred technical solution.As space is limited, exist
This no longer tires out one by one states.
Detailed description of the invention
Fig. 1 is placenta tissue extract albumen ultraviolet scanning spectrum figure.
Fig. 2 is placenta tissue extract albumen Tricine-SDS-PAGE figure.
Specific embodiment
By extensive and in-depth research, obtain on the basis of a large number of experiments can be used in preparing cosmetics the present inventor
Composition, the composition includes placenta tissue extract, licoflavone and trehalose, the experimental results showed that, placenta tissue mentions
It takes object and licoflavone that there is significant synergy, the whitening effect in use process, Er Qieneng can not only be significantly increased
Enough significant whitening effects extended after stopping using are held time, and the combination of placenta tissue extract and trehalose has significantly
The moistening effect of enhancing.
Include placenta tissue extract in composition of the invention, contains various active object needed for human metabolism
Matter plays an important role in terms of maintaining with reparation human normal physiological function.Anti- black contained by placenta tissue extract
Prime factor, moisturizing factor, transforming growth factor etc. have powerful moisturizing, whitening and anti-wrinkle effect, can fundamentally delay people
Body aging.It, can be with more importantly the hormonal substances such as the HCG, HPL contained in pig placenta, progesterone are well below Human plactnta
The thinning enhancing of the sensibility caused by human skin of cosmetics containing steroids, cuticula, hair follicle thickening, pore is avoided to increase, swash
The side effects such as plain dependence facial dermatitis.The present invention adds a variety of with enhancing placenta using pig placenta tissue extract as primary raw material
The effect of tissue extract moisturizing, whitening, anti-wrinkle effect ingredient, and be added and promote Transdermal absorption ingredient, manufactured cosmetics are protected
Wet, whitening, anti-wrinkle effect are excellent.
Before describing the present invention, it should be understood that the present invention is not limited to the specific method and experiment conditions, because this
Class method and condition can change.It should also be understood that its purpose of the term as used herein is only that description specific embodiment, and
And it is not intended to be restrictive, the scope of the present invention will be limited only by the claims which follow.
Unless otherwise defined, otherwise whole technologies used herein and scientific term all have such as fields of the present invention
The normally understood identical meanings of those of ordinary skill.As used herein, in use, term in mentioning the numerical value specifically enumerated
" about " mean that the value can be changed from the value enumerated and be not more than 1 parts by weight.For example, as used herein, statement " about 100 " includes 99
And 101 and between whole values (for example, 99.1,99.2,99.3,99.4 etc.).
Although can be used in implementation or test of the invention and heretofore described similar or of equal value any method
And material, place enumerates preferred method and material herein.
The first purpose of this invention is to provide a kind of cosmetics containing placenta tissue extract, mainly by following matter
Each ingredient of amount score is made:
Citric acid monoglyceride 1-5%, methylparaben 0.1-1%, propylben 0.1-1%, Phenoxyethanol 0.1-
1%, jojoba oil 1-10%, isopropyl palmitate 1-10%, Tween-60 1-5%, cetostearyl alcohol 1-5%, beeswax 1-
5%, atoleine 1-5%, Carbopol 941 0.1-0.5%, trehalose 1-10%, glycerol 1-10%, allantoin 0.1-
0.5%, hydroxyethyl cellulose 0.1-1%, EDETATE SODIUM 0.01-0.5%, triethanolamine 0.01-0.5%, myristic acid isopropyl
Ester 1-10%, licoflavone 0.1-1%, essence 0.01-0.1%, placenta tissue extract 0.1-5%, surplus are distilled water.Its
In, percentage is by weight.
It is preferably carried out in mode at of the invention one, the placenta tissue extract is pig placenta tissue extract.
It is preferably carried out in mode at of the invention one, the preparation method of the placenta tissue extract includes following step
It is rapid:
Naturally produced placenta is taken, umbilical cord, other kinds film are removed, repeated flushing removes remained blood；
Clean placenta is put into 75% ethanol solution and is impregnated 1~3 hour, is then used normal saline flushing 2~3 times；
Placenta weighing, and mixed with physiological saline in 1:1 (w/w) ratio, it is placed in the tissue mashing machine of sterilizing, asepsis ring
It is smashed to pieces, 1~5 minute every time, is spaced 0.5~3 minute with 8000~12000 turns of speed under border, repeated 1~3 time；
Stamping is collected, liquid nitrogen is added, is subsequently placed under 4~10 DEG C of environment and melts completely, multigelation 2~3 times；
Lysate is collected, is centrifuged 10-50 minutes at 4 DEG C with 5000~8000 turns of speed, supernatant is collected；
Supernatant is placed in 50~80 DEG C of water-baths 1~5 hour；
Placenta tissue extract then is can be obtained by 0.22 μm of filter membrane in supernatant.
It is preferably carried out in mode at of the invention one, the cosmetics are mainly by each ingredient system of following mass fraction
At:
Citric acid monoglyceride about 1.8%, methylparaben about 0.2%, propylben about 0.1%, Phenoxyethanol are about
0.3%, jojoba oil about 3%, isopropyl palmitate about 3.5%, Tween-60 about 1.5%, cetostearyl alcohol about 2.5%, beeswax
About 3%, atoleine about 2%, Carbopol 941 about 0.2%, trehalose about 3.2%, glycerol about 4%, allantoin about 0.2%, hydroxyl
Ethyl cellulose about 0.3%, EDETATE SODIUM about 0.1%, triethanolamine about 0.1%, isopropyl myristate about 5.2%, Radix Glycyrrhizae
Flavones about 0.2%, essence about 0.035%, placenta tissue extract about 0.5%, surplus are distilled water.
Second object of the present invention is the provision of a kind of preparation method of cosmetics, comprising the following steps:
By citric acid monoglyceride, methylparaben, propylben, Phenoxyethanol, jojoba oil, isopropyl palmitate,
Tween-60, cetostearyl alcohol, beeswax, atoleine are heated to 65~75 DEG C, mix, obtain component A；
By hydroxyethyl cellulose, distilled water, be heated to 80~90 DEG C, stir it is transparent to solution, flowing water be cooled to 40 DEG C with
Under, then Carbopol 941, trehalose, glycerol, allantoin, EDETATE SODIUM, placenta tissue extract are added, it mixes, obtains component B；
Component A is cooled to 40 DEG C hereinafter, component B is then added to component A, and sequentially add three ethyl alcohol under stiring
Amine, isopropyl myristate, licoflavone, essence.
In the preparation method, by citric acid monoglyceride, methylparaben, propylben, Phenoxyethanol, Jojoba
Oil, isopropyl palmitate, Tween-60, cetostearyl alcohol, beeswax, atoleine are heated to 70 DEG C；
It is preferably carried out in mode at of the invention one, in the preparation method, hydroxyethyl cellulose is heated to
85℃。
It is preferably carried out in mode at of the invention one, in the preparation method, flowing water is cooled to 40 DEG C.
Third object of the present invention be to provide cosmetics described in first purpose in preparation moisturizing cosmetic
Using.
Fourth object of the present invention be to provide cosmetics described in first purpose in preparing skin-lightening cosmetic
Using.
Fourth object of the present invention be to provide cosmetics described in first purpose in preparing anti-wrinkle cosmetic
Using.
Of the invention the 5th be designed to provide cosmetics described in first purpose preparation can moisturizing and beauty
The white application with anti-wrinkle cosmetic.
Cosmetics of the invention contain placenta tissue extract, licoflavone, trehalose and other functional ingredient, functional component
Between show significant synergy, addition hydroxyethyl cellulose, Carbopol 941 etc. form the body of cosmetics stable uniform
System.
Main advantages of the present invention are:
(1) cosmetic composition provided by the invention contain placenta tissue extract, licoflavone, trehalose and other effects at
Point, significant synergy is shown between functional component, is obviously improved at moisturizing, whitening and crease-resistant aspect.
(2) cosmetics preparation method provided by the invention, cosmetics obtained, stable uniform, no layering, demulsification etc. are existing
As.
Combined with specific embodiments below, the further old present invention in detail.It should be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.The experimental method of detailed conditions is not specified in the following example, usually according to conventional strip
Part such as U.S. Sambrook.J etc. writes " Molecular Cloning: A Laboratory room guide " (Huang Peitang etc. is translated, Beijing: Science Press, 2002)
Described in condition, or according to the normal condition proposed by manufacturer.Unless otherwise stated, otherwise percentage and number be by weight
It calculates.Experimental material used in following embodiment and reagent can obtain unless otherwise instructed from commercially available channel.
Embodiment 1
A kind of cosmetics containing placenta tissue extract are made of each ingredient of following mass fraction:
Citric acid monoglyceride 1%, methylparaben 0.1%, propylben 0.1%, Phenoxyethanol 0.1%, Jojoba
Oily 3%, isopropyl palmitate 3%, Tween-60 3%, cetostearyl alcohol 3%, beeswax 3%, atoleine 3%, Carbopol 941
0.1%, trehalose 3%, glycerol 3%, allantoin 0.1%, hydroxyethyl cellulose 0.1%, EDETATE SODIUM 0.01%, triethanolamine
0.01%, isopropyl myristate 3%, licoflavone 0.1%, essence 0.01%, placenta tissue extract 3%, surplus are to steam
Distilled water.Above-mentioned each group is divided into 100%.
Embodiment 2
The placenta tissue extract in the cosmetics of embodiment 1 is pig placenta tissue extract, the pig placenta group
Knit the preparation method of extract the following steps are included:
Naturally produced pig placenta is taken, umbilical cord, other kinds film are removed, repeated flushing removes remained blood；
Clean placenta is put into 75% ethanol solution and is impregnated 1~3 hour, is then used normal saline flushing 2~3 times；
Placenta weighing, and mixed with physiological saline in 1:1 (w/w) ratio, it is placed in the tissue mashing machine of sterilizing, asepsis ring
It is smashed to pieces, 1~5 minute every time, is spaced 0.5~3 minute with 8000~12000 turns of speed under border, repeated 1~3 time；
Stamping is collected, liquid nitrogen is added, is subsequently placed under 4~10 DEG C of environment and melts completely, multigelation 2~3 times；
Lysate is collected, is centrifuged 10-50 minutes at 4 DEG C with 5000~8000 turns of speed, supernatant is collected；
Supernatant is placed in 50~80 DEG C of water-baths 1~5 hour；
Placenta tissue extract then is can be obtained by 0.22 μm of filter membrane in supernatant.
Embodiment 3
The preparation method of the cosmetics of embodiment 1, comprising the following steps:
By citric acid monoglyceride, methylparaben, propylben, Phenoxyethanol, jojoba oil, isopropyl palmitate,
Tween-60, cetostearyl alcohol, beeswax, atoleine are heated to 70 DEG C, mix, obtain component A；
By hydroxyethyl cellulose, distilled water, 85 DEG C are heated to, stirring is transparent to solution, and flowing water is cooled to 40 DEG C, then adds
Carbopol 941, trehalose, glycerol, allantoin, EDETATE SODIUM, placenta tissue extract mix, obtain component B；
Component A is cooled to 40 DEG C under stiring, and component B is then added to component A, and sequentially adds triethanolamine, meat
Isopropyl myristate, licoflavone, essence.
Embodiment 4
A kind of cosmetics containing placenta tissue extract are mainly made of each ingredient of following mass fraction:
Citric acid monoglyceride 1.2%, methylparaben 0.2%, propylben 0.1%, Phenoxyethanol 0.5%, flash
Bar oil 3.5%, isopropyl palmitate 4%, Tween-60 5%, cetostearyl alcohol 3%, beeswax 3%, atoleine 2%, card wave
Nurse 941 0.5%, trehalose 4%, glycerol 3%, allantoin 0.1%, hydroxyethyl cellulose 0.5%, EDETATE SODIUM 0.03%, three
Ethanol amine 0.1%, isopropyl myristate 3%, licoflavone 0.3%, essence 0.03%, placenta tissue extract 4%, surplus
For distilled water.Above-mentioned each group is divided into 100%.
Embodiment 5
A kind of cosmetics containing placenta tissue extract are mainly made of each ingredient of following mass fraction:
Citric acid monoglyceride 3%, methylparaben 1%, propylben 0.5%, Phenoxyethanol 1%, jojoba oil
5%, isopropyl palmitate 1%, Tween-60 5%, cetostearyl alcohol 5%, beeswax 5%, atoleine 5%, Carbopol 941
0.5%, trehalose 5%, glycerol 5%, allantoin 0.1%, hydroxyethyl cellulose 0.5%, EDETATE SODIUM 0.03%, triethanolamine
0.5%, isopropyl myristate 5%, licoflavone 1%, essence 0.03%, placenta tissue extract 3%, surplus are distillation
Water.Above-mentioned each group is divided into 100%.
Test example 1: placenta tissue extract B CA method measures protein content
Take the 10 μ l of placenta tissue extract prepared, by BCA determination of protein concentration kit (green skies biology,
P0012S type) operation instruction operation, protein content in different batches extract is measured, is repeated twice.As a result as follows:
Calibration curve equation: y=0.555x+0.096 (R2=0.998)
1 placenta tissue extract protein concentration of table
| Serial number | OD value | Protein concentration (mg/ml) |
| Sample 1 | 0.181 | 0.306 |
| Sample 2 | 0.180 | 0.303 |
| Sample 3 | 0.184 | 0.320 |
Test example 2: placenta tissue extract determined by ultraviolet spectrophotometry protein content
The placenta tissue extract prepared is taken, 50 μ l are taken, distilled water is added to be diluted to 500 μ l, then takes 200 μ l, 80 μ respectively
L adds distilled water to be diluted to 4000 μ l, and 20 times, 50 times of dilutions are made.Dilution uses ultraviolet specrophotometer (Thermo
Electron Corp.EV300 type) measurement, scanning wavelength is 190nm~500nm.The results are shown in attached figure 1.
Test example 3: placenta tissue extract Tricine-SDS-PAGE method protein molecular weight measure of spread
With reference to master thesis " horse placenta water-solubility protein extraction process and its healthcare function research " the (the 13rd to 14
Page) operation, the results are shown in attached figure 2.
Test example 4: cosmetics stability
It is formula with embodiment 1,4,5, according to cosmetics prepared by the method for embodiment 2,3, lotion is fine and smooth, and it is pure white, it applies
Fast, stingless excitation is absorbed in hand, face.
The cosmetics of preparation are put into 45 DEG C of insulating boxs and carry out high temperature storage test, separately sampled recovery in 0,1,2 month is extremely
It is observed after room temperature.It is put into progress low tempertaure storage test in -15 DEG C of refrigerator simultaneously.
The result shows that product is stablized, there is not phenomena such as layering, demulsification.
Test example 5: moistening effect test
It is provided by Hainan Grade A hospital, chooses 28 volunteers and participate in test, the age 25-45 years old, be randomly divided into 7 groups,
Wherein cosmetics are made using embodiment 1 in test group 1, and cosmetics are made using embodiment 4 in test group 2, and test group 3 uses implementation
Cosmetics are made in example 5, and control group 1 uses commercially available moisturizing glycerol, and control group 2 is to be not added with using the formula difference of embodiment 4
Placenta tissue extract and trehalose additive amount doubles, control group 3 is to be not added with using the formula difference of embodiment 4
The other components of licoflavone are consistent, and control group 4 is to be not added with the other components one of trehalose using the formula difference of embodiment 4
It causes.The present inventor has carried out large-scale verifying in the course of the research, to cosmetics prescription, and it is representative only to enumerate part herein
Test data.It smears daily 1 time, continuous 5 days, 4 days later without using any skin care item as the control period.
Experience face's moisturizing effect on probation is divided into 0-10 registration, and 0 indicates no effect, and 1-2 indicates slightly have effect, 3-4 table
It is shown with certain effect, 5-6 indicates medium, and 7-8 indicates that effect is good, and 9-10 indicates that effect is very good.Every day each group registration data
Take mean value (round number).
Test result is as follows:
The moistening effect of 2 cosmetics of table
Above-mentioned test result shows that the cosmetic sampler effect of embodiment 4,5 is relatively good, deactivates in 3 days, still feels
Moistening effect.Compared with control group 1, moistening effect is significant.Also, it is found by experiment that, the cosmetics (control without trehalose
The moistening effect of group 4) significantly reduces, and the moistening effect of the cosmetics (control group 2) without placenta tissue extract also has reduction,
Illustrate that placenta tissue extract and trehalose have significant synergistic effect, placenta tissue extract and sea in terms of moistening effect
The collaboration of both algae sugar can effectively improve the moistening effect of cosmetics.
Test example 6: whitening effect test
It is provided by Hainan Grade A hospital, chooses 28 volunteers and participate in test, the age 25-45 years old, be randomly divided into 7 groups,
Wherein cosmetics are made using embodiment 1 in test group 1, and cosmetics are made using embodiment 4 in test group 2, and test group 3 uses implementation
Cosmetics are made in example 5, and control group 1 uses commercially available fresh milk, and control group 2 is to be not added with tire using the formula difference of embodiment 4
Disk tissue extract and licoflavone additive amount doubles, control group 3 is to be not added with using the formula difference of embodiment 4
Licoflavone, control group 4 be to be not added with trehalose using the formula difference of embodiment 4, and the present inventor is in the course of the research, right
Cosmetics prescription has carried out large-scale verifying, only enumerates the representational test data in part herein.It smears 1 time daily, even
5 days continuous, 4 days later without using any skin care item as the control period.
Experience face's whitening effect on probation is divided into 0-10 registration, and 0 indicates no effect, and 1-2 indicates slightly have effect, 3-4 table
It is shown with certain effect, 5-6 indicates medium, and 7-8 indicates that effect is good, and 9-10 indicates that effect is very good.Every day each group registration data
Take mean value (round number).
Test result is as follows:
The whitening effect of 3 cosmetics of table
Above-mentioned test result shows that the cosmetic sampler whitening effect of embodiment 5 is relatively good, deactivates in 4 days, still feels
To whitening effect.Compared with control group 1, whitening effect is significant.Although control group 2 also shows certain whitening effect,
It is after stopping using, whitening effect slackens rapidly.The whitening effect after stopping using of control group 3 can continue 3 days or so, but
It is that the whitening effect shown in use is bad.And in test group, it is not only shown in use process excellent
Whitening effect still be able to maintain certain whitening effect and 4 days after stopping using.The above results illustrate placenta group
Knit extract and licoflavone has significant synergistic effect in terms of whitening effect, can not only improve the beauty in use process
White effect, and certain whitening effect is also able to maintain that after stopping using.
Test example 7: anti-wrinkle effect test
It is provided by Hainan Grade A hospital, chooses 18 volunteers and participate in test, the age 25-45 years old, be randomly divided into 6 groups,
Wherein cosmetics are made using embodiment 1 in test group 1, and cosmetics are made using embodiment 4 in test group 2, and test group 3 uses implementation
Cosmetics are made in example 5, and control group 1 is to be not added with placenta tissue extract using the formula difference of embodiment 4, and control group 2 makes
It is to be not added with licoflavone with the formula difference of embodiment 4, control group 3 is to be not added with using the formula difference of embodiment 4
Trehalose, the present inventor have carried out large-scale verifying in the course of the research, to cosmetics prescription, and only enumerating part herein has generation
The test data of table.It smears daily 1 time, continuous 5 days, 4 days later without using any skin care item as the control period.
Experience face's anti-wrinkle effect on probation is divided into 0-10 registration, and 0 indicates no effect, and 1-2 indicates slightly have effect, 3-4 table
It is shown with certain effect, 5-6 indicates medium, and 7-8 indicates that effect is good, and 9-10 indicates that effect is very good.Every day each group registration data
Take mean value (round number).
Test result is as follows:
The anti-wrinkle effect of 4 cosmetics of table
Above-mentioned test result shows that the cosmetic sampler anti-wrinkle effect of embodiment 4 is relatively good, deactivates in 3 days, still feels
To anti-wrinkle effect.Control group 1 the result shows that, placenta tissue extract is affected to crease-resistant, mentions in no placenta tissue
Take anti-wrinkle effect in the case where object bad.
All references mentioned in the present invention is incorporated herein by reference, independent just as each document
It is incorporated as with reference to such.In addition, it should also be understood that, after reading the above teachings of the present invention, those skilled in the art can
To make various changes or modifications to the present invention, such equivalent forms equally fall within model defined by the application the appended claims
It encloses.
Claims (10)
1. a kind of composition, which is characterized in that the composition includes placenta tissue extract and licoflavone.
2. composition as described in claim 1, which is characterized in that the composition is cosmetic composition or pharmaceutical composition
Object.
3. composition as described in claim 1, which is characterized in that the composition further includes trehalose.
4. composition as described in claim 1, which is characterized in that include: in the composition
Placenta tissue extract 0.1-5 parts by weight；
Licoflavone 0.1-1 parts by weight；With
Trehalose 1-10 parts by weight.
5. composition as described in claim 1, which is characterized in that the composition further includes selected from the group below one or more
Component:
Citric acid monoglyceride, methylparaben, propylben, Phenoxyethanol, jojoba oil, isopropyl palmitate, tween-
60, cetostearyl alcohol, beeswax, atoleine, Carbopol 941, glycerol, allantoin, hydroxyethyl cellulose, EDETATE SODIUM, three second
Hydramine, isopropyl myristate and essence.
6. composition as described in claim 1, which is characterized in that the composition includes:
Citric acid monoglyceride 1-5 parts by weight, methylparaben 0.1-1 parts by weight, propylben 0.1-1 parts by weight, benzene oxygen second
Alcohol 0.1-1 parts by weight, jojoba oil 1-10 parts by weight, isopropyl palmitate 1-10 parts by weight, Tween-60 1-5 parts by weight, ten
Six octadecyl alcolol 1-5 parts by weight, beeswax 1-5 parts by weight, atoleine 1-5 parts by weight, Carbopol 941 0.1-0.5 parts by weight, sea
Algae sugar 1-10 parts by weight, glycerol 1-10 parts by weight, allantoin 0.1-0.5 parts by weight, hydroxyethyl cellulose 0.1-1 parts by weight,
EDETATE SODIUM 0.01-0.5 parts by weight, triethanolamine 0.01-0.5 parts by weight, isopropyl myristate 1-10 parts by weight, Radix Glycyrrhizae are yellow
Ketone 0.1-1 parts by weight, essence 0.01-0.1 parts by weight and placenta tissue extract 0.1-5 parts by weight.
7. composition as described in claim 1, which is characterized in that the placenta tissue extract is behaved or the placenta tissue of pig
Extract.
8. composition as described in claim 1, which is characterized in that the preparation method of the placenta tissue extract includes step
It is rapid:
(1) it pre-processes
Clean placenta is put into ethanol solution and is impregnated 1~3 hour, normal saline flushing is then used；
(2) it smashs to pieces
It is smashed to pieces after pretreated placenta is mixed with physiological saline, obtains stamping；
(3) freeze thawing
Stamping is cracked using liquid nitrogen multigelation 2~5 times, obtained lysate；
(4) it is centrifuged
Lysate is centrifuged, supernatant is collected；
(5) it keeps the temperature
Supernatant is placed under 50~80 DEG C of environment 1~5 hour, the placenta tissue extract is obtained.
9. a kind of preparation method of cosmetics, which is characterized in that comprising steps of
(I) by citric acid monoglyceride, methylparaben, propylben, Phenoxyethanol, jojoba oil, isopropyl palmitate, spit
Temperature -60, cetostearyl alcohol, beeswax and atoleine are heated to 65~75 DEG C, mix, obtain component A；
(II) hydroxyethyl cellulose and distilled water are mixed, is heated to 80~90 DEG C, stirring is transparent to solution, is cooled to 40 DEG C
Hereinafter, adding Carbopol 941, trehalose, glycerol, allantoin, EDETATE SODIUM, placenta tissue extract again, mixes, obtain component
B；
(II) component A is cooled to 40 DEG C hereinafter, component B is then added to component A, and sequentially add three ethyl alcohol under stiring
Amine, isopropyl myristate, licoflavone and essence.
10. composition described in claim 1 is preparing the application in cosmetics or drug.
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