CN101288639A - Method for purifying pearl hydrolysate using hyperfiltration and nanofiltration - Google Patents
Method for purifying pearl hydrolysate using hyperfiltration and nanofiltration Download PDFInfo
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- CN101288639A CN101288639A CNA2008101078756A CN200810107875A CN101288639A CN 101288639 A CN101288639 A CN 101288639A CN A2008101078756 A CNA2008101078756 A CN A2008101078756A CN 200810107875 A CN200810107875 A CN 200810107875A CN 101288639 A CN101288639 A CN 101288639A
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Abstract
The invention discloses a method for purifying pearl hydrolyzate by adoptng ultra-filtration and nano-filtration, pearls are smashed into powder, water is added, the temperature raises, and then phosphate buffer solution is used for regulating the pH value; a neutral protease is added in the solution to carry out the enzymatic hydrolysis reaction with pearl powder, the filtration is carried out when the solution is hot, thus obtaining clarified liquid of the pearl hydrolyzate; the filtration is carried out by using a hollow fiber ultra-filtration membrane with the molecular weight of 100000; and the filtration is further carried out by using a nano-filtration device with the molecular weight of 200, thus obtaining the pearl hydrolyzate without inorganic salts, enzyme and other impurities. The method for purifying the pearl hydrolyzate by adoptng ultra-filtration and nano-filtration provided by the invention has simple process and low cost; by adopting the mode of ultra-filtration and nano-filtration, the purity of the pearl hydrolyzate can be effectively improved, a product system can be avoided being damaged by the pearl hydrolyzate and the utilization rate of the active ingredients in the peals can be improved.
Description
Technical field
The present invention relates to a kind of purification process of hydrolyzed solution, be specifically related to a kind of method that adopts ultrafiltration and nanofiltration purifying pearl hydrolysate.
Background technology
The several amino acids and the trace element that contain needed by human body in the Margarita have nourishing blood to tranquillize the mind, relieving palpitation arresting convulsion, heat clearing away YIN nourishing, make eye bright, skin moistening pleases effects such as face.It is a kind of aesthetic health care good merchantable brand that is acknowledged as.The effective ingredient that utilizes Margarita at present mainly is to carry out with the form of Margarita hydrolyzed solution, as the interpolation material of nutrition, medical treatment, food, absorption of human body Margarita effective ingredient rate is improved greatly, has greatly expanded the range of application of Margarita.Existing Margarita hydrolyzed solution preparation method mainly is with mineral acid or organic acid Margarita powder to be dissolved, and adds enzyme again and is hydrolyzed, and obtains hydrating solution or directly spray drying oven dry.Owing in preparation process, add acid and carry out chemical reaction, contain a large amount of inorganic salts in the Margarita hydrolyzed solution; Adding enzyme is hydrolyzed, because enzyme also is a protein, the enzyme that was inactivated will be left over down a large amount of heteroproteins, like this, the composition of Margarita hydrolyzed solution is just indeterminate, a large amount of salts in the hydrolyzed solution can produce destruction to cosmetics oil-in-water type mastic, cause that the utilization rate of effective ingredient reduces greatly in the Margarita.
Summary of the invention
The objective of the invention is to provide a kind of method of purifying pearl hydrolysate at the deficiencies in the prior art, it is to adopt ultrafiltration and way of NF to improve the purity of Margarita hydrolyzed solution, avoid the destruction of Margarita hydrolyzed solution, improve the utilization rate of effective ingredient in the Margarita product system.
The technical solution adopted in the present invention is: with crushing pearl is powdery, crosses 1000 purpose screen clothes, adds water, is heated to 50～55 ℃ of temperature, with phosphate buffered solution pH value is adjusted to 6.0～7.5 then; Neutral protease added in the solution carried out enzymatic hydrolysis reaction 4～6 hours, separate with 250 mesh filter screen filtered while hot with Margarita powder, the clear liquor of Margarita hydrolyzed solution; With molecular weight is that 100,000 hollow fiber ultrafiltration membrane are filtered, and molecular weight is comprised the removal of enzyme molecule at the protein more than 100,000; Be that 200 nanofiltration equipment filters with molecular weight then, various inorganic salts, sulfate ion, the chloride ion of molecular weight below 200 will be removed, and the free amino acid in the hydrolyzed solution, polypeptide will be retained, and so do not contained the Margarita hydrolyzed solution of impurity such as inorganic salt, enzyme.Described neutral protease is a serrapeptass.
The method technology of employing ultrafiltration provided by the present invention and nanofiltration purifying pearl hydrolysate is simple, cost is low, adopt ultrafiltration and way of NF to improve the purity of Margarita hydrolyzed solution effectively, avoid the destruction of Margarita hydrolyzed solution, improve the utilization rate of effective ingredient in the Margarita product system.
The specific embodiment
The invention will be further described with indefiniteness embodiment below.
Embodiment one,
In the 100L enamel reactor, add deionized water 40kg, Margarita powder 10kg, add L-lactic acid 20kg, be warming up to 98 ℃, allow the abundant dissolved pearl powder of lactic acid, while hot gained solution is put into centrifuge, 4000 rev/mins.Remove supernatant need not, obtain the conchiolin of 0.5kg.The conchiolin of gained is put back in the enamel reactor, add deionized water 7.5L, be warming up to 50 ℃, slowly add phosphate buffer while stirring, pH value is transferred to 7.1, add serrapeptass 0.2kg, reinforced 54 ℃ of the constant temperature of finishing reacted discharging while hot 5 hours, feed liquid is poured the separation of 250 purpose strainer filterings into, gets 7.9kg Margarita hydrolyzed solution clear liquor.Be heated to 100 ℃, constant temperature enzyme denaturing half an hour.Upper prop separates, the first order, the hollow fiber ultrafiltration membrane ultrafiltration apparatus of last molecular cut off 100,000, post is pressed 1.5M, flow velocity 200L/h, molecular weight is that 100,000 above range protein will be given it up, obtain the Margarita hydrolyzed solution of 7.3kg. the second level, last molecular cut off 200 NF membrane, various inorganic salts, sulfate ion, the chloride ion of molecular weight below 200 will be trapped, simultaneously, Margarita hydrolyzed solution can also obtain certain concentrating.Promptly get the Margarita hydrolyzed solution of 6.3kg.The Margarita hydrolyzed solution ionic strength of gained is low, does not contain inorganic salt, and definite ingredients does not have the molecule protein of impurity such as enzyme.
Embodiment two,
In the 100L enamel reactor, add deionized water 50kg, Margarita powder 15kg, be warming up to 55 ℃, slowly add phosphate buffer while stirring, pH value is transferred to 6.5, add serrapeptass 0.2kg, reinforced 54 ℃ of the constant temperature of finishing, reacted 5 hours, discharging while hot, feed liquid are poured 250 purpose strainer filterings into and are separated, and get 53.4kg Margarita hydrolyzed solution clear liquor.Be heated to 100 ℃, constant temperature enzyme denaturing half an hour.Upper prop separates, the first order, the hollow fiber ultrafiltration membrane ultrafiltration apparatus of last molecular cut off 100,000, post is pressed 1.5M, flow velocity 200L/h, molecular weight is that 100,000 above range protein will be given it up, obtain the Margarita hydrolyzed solution of 37.3kg. the second level, last molecular cut off 200 NF membrane, various inorganic salts, sulfate ion, the chloride ion of molecular weight below 200 will be trapped, simultaneously, Margarita hydrolyzed solution can also obtain certain concentrating.Promptly get the Margarita hydrolyzed solution of 38.6kg.The Margarita hydrolyzed solution ionic strength of gained is low, does not contain inorganic salt, and definite ingredients does not have the molecule protein of impurity such as enzyme.
Claims (2)
1, a kind of method that adopts ultrafiltration and nanofiltration purifying pearl hydrolysate, it is characterized in that: with crushing pearl is powdery, crosses 1000 purpose screen clothes, adds water, is heated to 50～55 ℃ of temperature, with phosphate buffered solution pH value is adjusted to 6.0～7.5 then; Neutral protease added in the solution carried out enzymatic hydrolysis reaction 4～6 hours, separate with 250 mesh filter screen filtered while hot with Margarita powder, the clear liquor of Margarita hydrolyzed solution; With molecular weight is 100,000 hollow fiber ultrafiltration membrane filtering clarifying liquids, and molecular weight is comprised the removal of enzyme molecule at the protein more than 100,000; Be that 200 nanofiltration equipment filters with molecular weight then, various inorganic salts, sulfate ion, the chloride ion of molecular weight below 200 will be removed, and the free amino acid in the hydrolyzed solution, polypeptide will be retained, and do not contained the Margarita hydrolyzed solution of impurity such as inorganic salt, enzyme.
2, the method for employing ultrafiltration according to claim 1 and nanofiltration purifying pearl hydrolysate, it is characterized in that: described neutral protease is a serrapeptass.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101078756A CN101288639A (en) | 2008-05-23 | 2008-05-23 | Method for purifying pearl hydrolysate using hyperfiltration and nanofiltration |
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| CNA2008101078756A CN101288639A (en) | 2008-05-23 | 2008-05-23 | Method for purifying pearl hydrolysate using hyperfiltration and nanofiltration |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103804468A (en) * | 2014-02-19 | 2014-05-21 | 海南京润珍珠生物技术股份有限公司 | Preparation method of nano pearl proteins |
| CN104905242A (en) * | 2014-03-14 | 2015-09-16 | 浙江欧诗漫生物股份有限公司 | A method for preparing pearl separated pearl soluble edible calcium and pearl compound whitening factor solution |
| CN104911239A (en) * | 2014-03-14 | 2015-09-16 | 浙江欧诗漫生物股份有限公司 | Method for enzymolysis separation of pearl active polypeptide from pearl protein |
| CN105640867A (en) * | 2016-01-30 | 2016-06-08 | 海南京润珍珠生物技术股份有限公司 | Facial mask for replenishing moisture intensively with pearl essence and preparation method of facial mask |
| CN114403437A (en) * | 2022-01-27 | 2022-04-29 | 海南久常制药有限公司 | A method for preparing Margarita powder |
| WO2024000089A1 (en) * | 2022-06-27 | 2024-01-04 | 欧诗漫生物股份有限公司 | Method for preparing pearl-whitening and anti-aging combined polypeptide and single whitening polypeptide and use thereof |
-
2008
- 2008-05-23 CN CNA2008101078756A patent/CN101288639A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103804468A (en) * | 2014-02-19 | 2014-05-21 | 海南京润珍珠生物技术股份有限公司 | Preparation method of nano pearl proteins |
| CN104905242A (en) * | 2014-03-14 | 2015-09-16 | 浙江欧诗漫生物股份有限公司 | A method for preparing pearl separated pearl soluble edible calcium and pearl compound whitening factor solution |
| CN104911239A (en) * | 2014-03-14 | 2015-09-16 | 浙江欧诗漫生物股份有限公司 | Method for enzymolysis separation of pearl active polypeptide from pearl protein |
| CN105640867A (en) * | 2016-01-30 | 2016-06-08 | 海南京润珍珠生物技术股份有限公司 | Facial mask for replenishing moisture intensively with pearl essence and preparation method of facial mask |
| CN114403437A (en) * | 2022-01-27 | 2022-04-29 | 海南久常制药有限公司 | A method for preparing Margarita powder |
| WO2024000089A1 (en) * | 2022-06-27 | 2024-01-04 | 欧诗漫生物股份有限公司 | Method for preparing pearl-whitening and anti-aging combined polypeptide and single whitening polypeptide and use thereof |
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Open date: 20081022 |