CN103782771A - Fast root taking and seedling growing method for patchouli - Google Patents
Fast root taking and seedling growing method for patchouli Download PDFInfo
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- CN103782771A CN103782771A CN201410039484.0A CN201410039484A CN103782771A CN 103782771 A CN103782771 A CN 103782771A CN 201410039484 A CN201410039484 A CN 201410039484A CN 103782771 A CN103782771 A CN 103782771A
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Abstract
The invention relates to a semi-lignified twig cutting and fast root taking and seedling growing method for patchouli. The method comprises the key links of scion-seed stock plant selection, scion picking, root speeding treatment, cutting medium preparation, cutting and cutting post-management. According to the method, the cutting seedlings of the patchouli can root in advance, roots can be taken fast, the cutting survival rate can reach more than 99 percent, sprouting only needs 15 days, and cutting and seedling growing can be carried out all the year round. The method is easy to operate and popularize, a large number of high-quality patchouli seedlings can be cultured in a short time, and a good foundation is laid for the development of the patchouli industry.
Description
Technical field
The invention belongs to plant asexual multiplication technology field, be specifically related to a kind of method that Pogostemon cablin Rapid Rooting is grown seedlings.
Background technology
Pogostemon cablin [
pogostemon cablin(Blanco) Benth.] be that Labiatae thorn stamen grass belongs to herbaceous plant, slightly warm in nature, taste are pungent, have eliminating dampness with aromatics, the preventing or arresting vomiting that whets the appetite, deliver effect of relieving summer-heat.Pogostemon cablin is not only the primary raw material of more than 30 kind of Chinese patent drug, and with the Pogostemon cablin essential of its extraction, or medicine and the important source material of light industry industry (as preparation pellet, cream, ball, fall apart and cosmetics, fixastive and agrochemical), among the people also with Pogostemon cablin leaf decoct drink avoid heat wet.Pogostemon cablin plant belongs to Tropical Asian kind as a natural classification group, blooms China producing region is rare, fails solidly always, over over thousands of year, does not meet sexual propagation, therefore can only adopt tissue cultivation or vegetative propagation.
From Reproduction Conditions and the practicality that popularizes, Pogostemon cablin tissue culture propagating is a technical very strong job, and infrastructure has high input, aseptic condition requirement is high, the hardening cycle is long, process is loaded down with trivial details.
Pogostemon cablin cottage propagation has the features such as simple to operate, reproduction speed is fast, cost is low, therefore produces the upper cottage propagation that adopts more.But there is following defect in traditional cottage propagation: 1) cuttage envirment factor is wayward; 2) cuttings selection and classification are not strict; 3) the cuttage rooting time is 12-15 days, slower; 4) cuttings processing mode is improper; 5) cuttings is easily encroached on by germ and is caused rotten.Thereby easily waste Pogostemon cablin branch and cuttings resource or cause cuttage seeding survival rate low.On December 25th, 2002, on " Chinese traditional Chinese medicine report ", deliver " Pogostemon cablin propagation method " literary composition, but still exist, cutting time is limited, survival rate is on the low side, seedling is of low quality, waste the problems such as more branch and cuttings.Therefore, urgently provide that a kind of seedling is fast, survival rate is high and save again the Pogostemon cablin cuttage and seedling culture method of branch resource.
State Intellectual Property Office also discloses " quick dipping-rooting agent for woody plant " patent by name, number of patent application is 200510045674.4, although there is higher cuttage survival rate, but through repeatedly repeatedly practising, its processing time of taking root is longer, cuttage seeding is disease-resistant, diseases prevention ability and growth rate slower.Thereby, develop that a kind of seedling is fast, survival rate is high and save again the Pogostemon cablin cuttage and seedling culture method of branch resource, be extremely necessary.
Summary of the invention
In order to overcome the deficiencies in the prior art, the object of the present invention is to provide and a kind ofly can improve cuttage survival rate and the fast Pogostemon cablin cuttage and seedling culture method of seedling.By the application of the method, cuttage rooting is fast, rooting rate is high, the amount of taking root is large, and root system is full sturdy, has greatly shortened the cuttage and seedling culture time, has improved the survival rate of cuttage seeding, can breed in a short time a large amount of high-quality Pogostemon cablin seedlings.
In order to achieve the above object, the technical solution adopted in the present invention is: a kind of Pogostemon cablin Rapid Rooting seedling-cultivating method, the method comprises the steps:
1) adopt the selection of fringe maternal plant: choose robust growth, grow fine, without the Pogostemon cablin plant of damage by disease and insect as adopting fringe maternal plant; 2) adopt fringe: adopt fringe first 7 days, 14 days, respectively spray and adopt fringe maternal plant 1 time with the potassium dihydrogen phosphate that 800 times of liquid+mass fractions of 70% thiophanate methyl are 0.2% respectively.Choose top and make cuttings with the semi-lignified spray of 2 great Ye and 2 little lobus cardiacuses, clip cuttings 8-10cm, sheared edge is 40-50 degree and level and smooth; 3) urge root processing: 30 of cuttings after clip are bundled into one, be immersed in the naphthalene acid solution that mass fraction is 0.01%-0.03%, soaking depth is 2-3cm, and 6-10 takes out after second; 4) preparation of cutting medium: the matrix that cuttage is used adopts coconut palm chaff, river sand and loam, prepare and mix thoroughly in 1 ︰ 1 ︰ 1 ratio, keep the humidity of 40%-50%, utilize 80 ℃ of above high-temperature water vapors to sterilize 1 hour, after matrix is naturally cooling, be laid on slotting machine, thickness is 8-10cm; 5) cuttage: will urge root cuttings after treatment, insert matrix, the 1/3-1/2 that the degree of depth is cutting length, density 4cm × 4cm, after cuttage, water immediately permeable, keep matrix moistening; 6) after cuttage, manage: timing spraying every day after cuttage.Wherein, 1-5 days after inserting, callus forms gradually, spraying in every 25-30 minute 25 seconds; 6-10 days after inserting, cuttings is taken root gradually, spraying in every 40-50 minute 25 seconds; 11-15 days after inserting, cuttage rooting is substantially complete, spraying in every 70-80 minute 25 seconds.Cuttage and seedling culture temperature of shed is controlled at 25-35 ℃, and relative moisture is at 85%-95%, and when outdoor intensity of illumination exceedes 10000LX, covered with sunshade net shelters from heat or light.
In order to prevent that the wound of cuttings is infected and improves rooting rate, in step 3) urge root processing before to cuttings disinfection, processing method is: first do not have sheared edge 30 minutes by 800 times of immersions of 70% thiophanate methyl, more clean with clear water.
In order to guarantee the survival rate of cuttings, cuttings adopts perpendicular inserting, the joint of the cuttings matrix of submerging when perpendicular inserting, and blade is exposed in air.
Growing seedlings the maintenance of temperature of shed can be by overlay film, shade, ventilate or spray realization.
The invention has the advantages that: 1) the traditional cuttage and seedling culture of Pogostemon cablin be clip maternal plant branch end be about 15-20cm branch, as propagating materials, but like this maternal plant impact of growing is destroyed greatly, have a strong impact on quantity and quality then, and waste a large amount of branches and cuttings.The present invention only need choose the long semi-lignified spray of 8-10cm as cuttings, has saved Pogostemon cablin branch and cuttings resource; 2) the present invention adopts high-temperature steam sterilization cutting medium, can effectively kill intramatrical various harmful microorganism and weed seed, and cuttage seeding is difficult for growing pathogen, reduces the harm of disease worm, reduces Pogostemon cablin cultivation management cost; 3) the root solution of urging of the present invention, is applied in Pogostemon cablin epicormic branch cutting, can effectively activate and form the thin race of layer, make wound form at short notice callus, impel adventitious root primordia burst to take root, cuttage survival rate is up to more than 99%, the seedling time is short, and throughout the year all can cuttage and seedling culture; 4) the present invention also has raw material and easily purchases, prepares the advantage such as stable easy, reliable for effect, and popularizing application prospect is wide.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail.
Embodiment 1: a kind of method that Pogostemon cablin Rapid Rooting is grown seedlings, the method comprises the steps:
1) adopt the selection of fringe maternal plant: in Haikou City University Of Hainan Pogostemon cablin Germplasm Resources, carried out the test of Pogostemon cablin cuttage and seedling culture in August, 2013.First choose robust growth, grow fine, without the Pogostemon cablin plant of damage by disease and insect as adopting fringe maternal plant; 2) adopt fringe: adopt fringe first 7 days, 14 days, respectively spray and adopt fringe maternal plant 1 time with the potassium dihydrogen phosphate that 800 times of liquid+mass fractions of 70% thiophanate methyl are 0.2% respectively.Choose top and make cuttings with the semi-lignified spray of 2 great Ye and 2 little lobus cardiacuses, clip cuttings 8-10cm, sheared edge is 40-50 degree and level and smooth.Then, do not have sheared edge 30 minutes by 800 times of immersions of 70% thiophanate methyl, to complete sterilizing, then clean with clear water.The compound method of 800 times of liquid of 70% thiophanate methyl is: take 70% thiophanate methyl wetting powder 1g, be watered 800ml, stirring forms; 3) urge root processing: 30 of cuttings after sterilization are bundled into one, be immersed in mass fraction and be in 0.01% naphthalene acid solution, soaking depth is 2-3cm, within 10 seconds, takes out afterwards.Mass fraction is that the compound method of 0.01% naphthalene acid solution is: take methyl α-naphthyl acetate 1.0g, be dissolved in the 75%(v/v of 50ml) in ethanolic solution, add water and be settled to 10L, after stirring, make; 4) preparation of cutting medium: the matrix that cuttage is used adopts coconut palm chaff, river sand and loam, prepare and mix thoroughly in 1 ︰ 1 ︰ 1 ratio, keep the humidity of 40%-50%, then matrix is piled to 25cm high, cover the Polypropylence Sheet of waterproof heat-resisting above, the steam of 85 ℃ that steam boiler is produced, be transported in matrix by pipeline, to matrix sterilization 1 hour, after matrix is naturally cooling, be laid on slotting machine, thickness is 8-10cm; 5) cuttage: will urge root cuttings after treatment, insert vertically matrix, the 1/3-1/2 that the degree of depth is cutting length, density 4cm × 4cm, the joint of the cuttings matrix of submerging when perpendicular inserting, blade is exposed in air.After cuttage, water immediately permeable, keep matrix moistening; 6) after cuttage, manage: timing spraying every day after cuttage.Wherein, 1-5 days after inserting, callus forms gradually, spraying in every 25-30 minute 25 seconds; 6-10 days after inserting, cuttings is taken root gradually, spraying in every 40-50 minute 25 seconds; 11-15 days after inserting, cuttage rooting is substantially complete, spraying in every 70-80 minute 25 seconds.Cuttage and seedling culture temperature of shed is controlled at 25-35 ℃, and the maintenance of temperature can be by overlay film, shade, ventilate or the realization of spraying, and relative moisture is at 85%-95%, and when outdoor intensity of illumination exceedes 10000LX, covered with sunshade net shelters from heat or light; 7) actual effect: 2000 of the present embodiment cuttage cuttings, actual effect is in table 1.
Embodiment 2: compared with embodiment 1, the difference of the present embodiment is: it is in 0.03% naphthalene acid solution that cuttings is immersed in mass fraction, takes out for 6 seconds afterwards; Vapor (steam) temperature to matrix sterilization is 90 ℃.Actual effect is in table 1.
Embodiment 3: compared with embodiment 1, the difference of the present embodiment is: it is in 0.02% naphthalene acid solution that cuttings is immersed in mass fraction, takes out for 8 seconds afterwards; Vapor (steam) temperature to matrix sterilization is 80 ℃.Actual effect is in table 1.
The above; it is only preferably embodiment of the present invention; protection scope of the present invention is not limited to this; for those skilled in the art; under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (4)
1. the method that Pogostemon cablin Rapid Rooting is grown seedlings, is characterized in that comprising following committed step:
1) adopt the selection of fringe maternal plant: choose robust growth, grow fine, without the Pogostemon cablin plant of damage by disease and insect as adopting fringe maternal plant; 2) adopt fringe: adopt fringe first 7 days, 14 days, respectively spray and adopt fringe maternal plant 1 time with the potassium dihydrogen phosphate that 800 times of liquid+mass fractions of 70% thiophanate methyl are 0.2% respectively, choosing top has the semi-lignified spray of 2 great Ye and 2 little lobus cardiacuses to make cuttings, clip cuttings 8-10cm, sheared edge is 40-50 degree and level and smooth; 3) urge root processing: 30 of cuttings after shearing are bundled into one, be immersed in the naphthalene acid solution that mass fraction is 0.01%-0.03%, soaking depth is 2-3cm, and 6-10 takes out after second; 4) preparation of cutting medium: the matrix that cuttage is used adopts coconut palm chaff, river sand and loam, prepare and mix thoroughly in 1 ︰ 1 ︰ 1 ratio, keep the humidity of 40%-50%, utilize 80 ℃ of above high-temperature water vapors to sterilize 1 hour, after matrix is naturally cooling, be laid on slotting machine, thickness is 8-10cm; 5) cuttage: will urge root cuttings after treatment, insert matrix, the 1/3-1/2 that the degree of depth is cutting length, density 4cm × 4cm, after cuttage, water immediately permeable, keep matrix moistening; 6) after cuttage, manage: timing spraying every day after cuttage, wherein, and 1-5 days after inserting, callus forms gradually, spraying in every 25-30 minute 25 seconds; 6-10 days after inserting, cuttings is taken root gradually, spraying in every 40-50 minute 25 seconds; 11-15 days after inserting, cuttage rooting is substantially complete, spraying in every 70-80 minute 25 seconds, the temperature of shed of growing seedlings is controlled at 25-35 ℃, and relative moisture is at 85%-95%, and when outdoor intensity of illumination exceedes 10000LX, covered with sunshade net shelters from heat or light.
2. Pogostemon cablin Rapid Rooting seedling-cultivating method according to claim 1, it is characterized in that, in step 3) urge root to process the cuttings disinfection to shearing before, processing method is: first do not have sheared edge 30 minutes by 800 times of immersions of 70% thiophanate methyl, more clean with clear water.
3. Pogostemon cablin Rapid Rooting seedling-cultivating method according to claim 1, is characterized in that described step 5) in cuttings adopt perpendicular inserting, the joint of the cuttings matrix of submerging when perpendicular inserting, blade is exposed in air.
4. Pogostemon cablin Rapid Rooting seedling-cultivating method according to claim 1, is characterized in that described step 6) in grow seedlings the maintenance of temperature of shed can be by overlay film, shade, ventilate or spray realization.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106069027A (en) * | 2016-06-16 | 2016-11-09 | 南京中医药大学 | The annual high-efficient method for cultivating that a kind of Herba Pogostemonis is potted plant |
| CN106472215A (en) * | 2016-09-30 | 2017-03-08 | 贵州山至金生态农业有限公司 | Peach tree nursery cuttage breeding method |
| CN108668655A (en) * | 2018-03-30 | 2018-10-19 | 湖北省农业科学院中药材研究所 | A kind of elegant jessamine seedling high-efficient method for cultivating |
| CN109220267A (en) * | 2018-10-09 | 2019-01-18 | 广东省中药研究所 | A kind of cuttage and seedling culture method of Shipai Huoxiang |
| CN111699943A (en) * | 2020-06-23 | 2020-09-25 | 贵港市农业科学研究院 | Agastache rugosa cutting seedling raising substrate and preparation method thereof |
| CN111990260A (en) * | 2020-09-22 | 2020-11-27 | 广东药科大学 | Tissue culture rapid propagation method of ketone type patchouli |
-
2014
- 2014-01-28 CN CN201410039484.0A patent/CN103782771B/en not_active Expired - Fee Related
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106069027A (en) * | 2016-06-16 | 2016-11-09 | 南京中医药大学 | The annual high-efficient method for cultivating that a kind of Herba Pogostemonis is potted plant |
| CN106472215A (en) * | 2016-09-30 | 2017-03-08 | 贵州山至金生态农业有限公司 | Peach tree nursery cuttage breeding method |
| CN108668655A (en) * | 2018-03-30 | 2018-10-19 | 湖北省农业科学院中药材研究所 | A kind of elegant jessamine seedling high-efficient method for cultivating |
| CN109220267A (en) * | 2018-10-09 | 2019-01-18 | 广东省中药研究所 | A kind of cuttage and seedling culture method of Shipai Huoxiang |
| CN111699943A (en) * | 2020-06-23 | 2020-09-25 | 贵港市农业科学研究院 | Agastache rugosa cutting seedling raising substrate and preparation method thereof |
| CN111990260A (en) * | 2020-09-22 | 2020-11-27 | 广东药科大学 | Tissue culture rapid propagation method of ketone type patchouli |
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