CN103764007B - 荧光观察装置和荧光观察系统以及荧光图像处理方法 - Google Patents

荧光观察装置和荧光观察系统以及荧光图像处理方法 Download PDF

Info

Publication number
CN103764007B
CN103764007B CN201280039584.2A CN201280039584A CN103764007B CN 103764007 B CN103764007 B CN 103764007B CN 201280039584 A CN201280039584 A CN 201280039584A CN 103764007 B CN103764007 B CN 103764007B
Authority
CN
China
Prior art keywords
image
fluorescence
reference light
light
divided
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201280039584.2A
Other languages
English (en)
Chinese (zh)
Other versions
CN103764007A (zh
Inventor
石原康成
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olympus Corp
Original Assignee
Olympus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olympus Corp filed Critical Olympus Corp
Publication of CN103764007A publication Critical patent/CN103764007A/zh
Application granted granted Critical
Publication of CN103764007B publication Critical patent/CN103764007B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • A61B1/04Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor combined with photographic or television appliances
    • A61B1/043Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor combined with photographic or television appliances for fluorescence imaging
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • A61B1/00002Operational features of endoscopes
    • A61B1/00004Operational features of endoscopes characterised by electronic signal processing
    • A61B1/00009Operational features of endoscopes characterised by electronic signal processing of image signals during a use of endoscope
    • A61B1/000095Operational features of endoscopes characterised by electronic signal processing of image signals during a use of endoscope for image enhancement
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • A61B1/06Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements
    • A61B1/0638Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements providing two or more wavelengths
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
    • A61B1/06Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements
    • A61B1/0646Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor with illuminating arrangements with illumination filters
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B23/00Telescopes, e.g. binoculars; Periscopes; Instruments for viewing the inside of hollow bodies; Viewfinders; Optical aiming or sighting devices
    • G02B23/24Instruments or systems for viewing the inside of hollow bodies, e.g. fibrescopes
    • G02B23/2407Optical details
    • G02B23/2461Illumination
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00Image enhancement or restoration
    • G06T5/90Dynamic range modification of images or parts thereof
    • G06T5/92Dynamic range modification of images or parts thereof based on global image properties

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Surgery (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Biomedical Technology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Medical Informatics (AREA)
  • Biophysics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Radiology & Medical Imaging (AREA)
  • Public Health (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Signal Processing (AREA)
  • Astronomy & Astrophysics (AREA)
  • Theoretical Computer Science (AREA)
  • Endoscopes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Instruments For Viewing The Inside Of Hollow Bodies (AREA)
CN201280039584.2A 2011-08-18 2012-08-08 荧光观察装置和荧光观察系统以及荧光图像处理方法 Expired - Fee Related CN103764007B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2011-178922 2011-08-18
JP2011178922A JP5816486B2 (ja) 2011-08-18 2011-08-18 蛍光観察装置および蛍光観察システム並びに蛍光観察装置の蛍光画像処理方法
PCT/JP2012/070241 WO2013024773A1 (ja) 2011-08-18 2012-08-08 蛍光観察装置および蛍光観察システム並びに蛍光画像処理方法

Publications (2)

Publication Number Publication Date
CN103764007A CN103764007A (zh) 2014-04-30
CN103764007B true CN103764007B (zh) 2016-04-13

Family

ID=47715093

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201280039584.2A Expired - Fee Related CN103764007B (zh) 2011-08-18 2012-08-08 荧光观察装置和荧光观察系统以及荧光图像处理方法

Country Status (5)

Country Link
US (1) US9182347B2 (https=)
EP (1) EP2745761B1 (https=)
JP (1) JP5816486B2 (https=)
CN (1) CN103764007B (https=)
WO (1) WO2013024773A1 (https=)

Families Citing this family (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
CA2865575C (en) 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions and kits for molecular counting
JP5677378B2 (ja) * 2012-07-25 2015-02-25 富士フイルム株式会社 内視鏡システム
EP2979611A4 (en) * 2013-03-29 2016-12-14 Olympus Corp FLUORESCENCE OBSERVATION DEVICE
JP6198426B2 (ja) * 2013-03-29 2017-09-20 浜松ホトニクス株式会社 蛍光観察装置及び蛍光観察方法
KR102758333B1 (ko) 2013-08-28 2025-01-23 벡톤 디킨슨 앤드 컴퍼니 대량의 동시 단일 세포 분석
US9582877B2 (en) * 2013-10-07 2017-02-28 Cellular Research, Inc. Methods and systems for digitally counting features on arrays
KR20170097653A (ko) 2014-12-17 2017-08-28 케어스트림 헬스 인코포레이티드 경구-내 3-d 형광 이미징
EP3259371B1 (en) 2015-02-19 2020-09-02 Becton, Dickinson and Company High-throughput single-cell analysis combining proteomic and genomic information
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
JP6110887B2 (ja) * 2015-03-17 2017-04-05 浜松ホトニクス株式会社 重畳画像生成装置及び重畳画像生成装置の作動方法
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
EP4582556A3 (en) 2015-04-23 2025-10-08 Becton, Dickinson and Company Methods and compositions for whole transcriptome amplification
EP3278717B1 (en) * 2015-05-20 2023-11-08 Sony Olympus Medical Solutions Inc. Signal processing device and medical observation system
WO2016196229A1 (en) 2015-06-01 2016-12-08 Cellular Research, Inc. Methods for rna quantification
KR101717362B1 (ko) * 2015-08-05 2017-03-16 왕용진 평면 스캔 비디오 카이모그라피와 후두 스트로보스코피 기능이 있는 비디오 후두 내시경 시스템
JP6940484B2 (ja) 2015-09-11 2021-09-29 セルラー リサーチ, インコーポレイテッド ライブラリー正規化のための方法および組成物
WO2017060954A1 (ja) 2015-10-05 2017-04-13 オリンパス株式会社 撮像装置
KR101771325B1 (ko) * 2015-10-29 2017-08-24 한국 한의학 연구원 적응 임계값 도출과 설태, 설질, 및 혼합 영역을 구분하는 장치 및 방법
JP7129343B2 (ja) 2016-05-02 2022-09-01 ベクトン・ディキンソン・アンド・カンパニー 正確な分子バーコーディング
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
KR101822969B1 (ko) * 2016-09-01 2018-01-31 한국과학기술연구원 근적외선과 cmos 영상 센서를 이용한 비침습 생체 이미징 방법 및 시스템
CN109791157B (zh) 2016-09-26 2022-06-07 贝克顿迪金森公司 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达
CN110382708A (zh) 2017-02-01 2019-10-25 赛卢拉研究公司 使用阻断性寡核苷酸进行选择性扩增
CN110719959B (zh) 2017-06-05 2021-08-06 贝克顿迪金森公司 针对单细胞的样品索引
EP3417758A1 (en) 2017-06-19 2018-12-26 Ambu A/S A method for processing image data using a non-linear scaling model and a medical visual aid system
TWI659395B (zh) * 2017-07-19 2019-05-11 AcuSolutions Inc. 類h&e影像之合成方法及採用該方法之光學系統
US10835116B2 (en) * 2017-11-16 2020-11-17 Karl Storz Imaging, Inc. Vocal cord stroboscopy
EP3728636B1 (en) 2017-12-19 2024-09-11 Becton, Dickinson and Company Particles associated with oligonucleotides
US10665000B2 (en) * 2018-01-08 2020-05-26 AcuSolutions Inc. Pseudo H and E image producing method and optical system using same
JP6856559B2 (ja) * 2018-01-23 2021-04-07 浜松ホトニクス株式会社 光測定装置及び光測定方法
KR102457527B1 (ko) * 2018-01-25 2022-10-21 한화정밀기계 주식회사 플립 칩의 플럭스 도포 상태 검사 방법
JP2019168423A (ja) * 2018-03-26 2019-10-03 浜松ホトニクス株式会社 画像取得装置及び画像取得方法
JP7358388B2 (ja) 2018-05-03 2023-10-10 ベクトン・ディキンソン・アンド・カンパニー 反対側の転写物末端における分子バーコーディング
CN112272710A (zh) 2018-05-03 2021-01-26 贝克顿迪金森公司 高通量多组学样品分析
CN112040831A (zh) * 2018-05-31 2020-12-04 松下i-PRO传感解决方案株式会社 相机设备、图像处理方法和相机系统
ES2992135T3 (es) 2018-10-01 2024-12-09 Becton Dickinson Co Determinar secuencias de transcripción 5’
US11932849B2 (en) 2018-11-08 2024-03-19 Becton, Dickinson And Company Whole transcriptome analysis of single cells using random priming
CN118542731A (zh) 2018-12-05 2024-08-27 直观外科手术操作公司 用于图像引导外科手术的照射校正的近红外(nir)成像
US11492660B2 (en) 2018-12-13 2022-11-08 Becton, Dickinson And Company Selective extension in single cell whole transcriptome analysis
CN109840912B (zh) * 2019-01-02 2021-05-04 厦门美图之家科技有限公司 一种图像中异常像素的修正方法及计算设备
US11371076B2 (en) 2019-01-16 2022-06-28 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
ES2945227T3 (es) 2019-01-23 2023-06-29 Becton Dickinson Co Oligonucleótidos asociados con anticuerpos
WO2020167920A1 (en) 2019-02-14 2020-08-20 Cellular Research, Inc. Hybrid targeted and whole transcriptome amplification
JP7290449B2 (ja) * 2019-04-03 2023-06-13 株式会社ディスコ 超高速度撮像装置
CN120099137A (zh) 2019-07-22 2025-06-06 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
JP7522189B2 (ja) 2019-11-08 2024-07-24 ベクトン・ディキンソン・アンド・カンパニー 免疫レパートリーシーケンシングのための完全長v(d)j情報を得るためのランダムプライミングの使用
KR20220133182A (ko) 2019-12-04 2022-10-04 덴탈 이미징 테크놀로지스 코퍼레이션 자동 차트작성을 동반한 구강내 3d 스캐닝
WO2021146219A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Cell capture using du-containing oligonucleotides
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
WO2021173719A1 (en) 2020-02-25 2021-09-02 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
CN111369478B (zh) * 2020-03-04 2023-03-21 腾讯科技(深圳)有限公司 人脸图像增强方法、装置、计算机设备和存储介质
US11661625B2 (en) 2020-05-14 2023-05-30 Becton, Dickinson And Company Primers for immune repertoire profiling
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
CN111803013A (zh) * 2020-07-21 2020-10-23 深圳市博盛医疗科技有限公司 一种内窥镜成像方法和内窥镜成像系统
US12391940B2 (en) 2020-07-31 2025-08-19 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
EP4247967A1 (en) 2020-11-20 2023-09-27 Becton, Dickinson and Company Profiling of highly expressed and lowly expressed proteins
CN116685850A (zh) 2020-12-15 2023-09-01 贝克顿迪金森公司 单细胞分泌组分析
WO2022147422A1 (en) 2020-12-31 2022-07-07 Stryker Corporation Systems and methods for low-light image enhancement
CN112967207B (zh) * 2021-04-23 2024-04-12 北京恒安嘉新安全技术有限公司 一种图像处理方法、装置、电子设备及存储介质
CN115406905B (zh) * 2021-05-28 2025-10-28 上海芯上微装科技股份有限公司 缺陷检测装置及缺陷检测的校正方法
US12239409B2 (en) 2022-02-28 2025-03-04 Visionsense Ltd. Fluorescence imaging camera assembly for open surgery
CN115299853A (zh) * 2022-08-31 2022-11-08 安徽省立医院(中国科学技术大学附属第一医院) 一种具有智能计算功能的4k荧光摄像系统
WO2024073291A2 (en) * 2022-09-26 2024-04-04 Life Technologies Corporation Normalization methods
EP4616781A1 (en) * 2024-03-14 2025-09-17 Quest Photonic Devices B.V. Method and system for visualization of faint fluorescence in surgery, software program
EP4616782A1 (en) * 2024-03-14 2025-09-17 Quest Photonic Devices B.V. Method and system for visualization of faint fluorescence in surgery, software program

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62247232A (ja) 1986-04-21 1987-10-28 Agency Of Ind Science & Technol 蛍光測定装置
JPS63252134A (ja) 1987-04-10 1988-10-19 工業技術院長 螢光検出を利用したがん診断装置
JP2001017379A (ja) * 1999-07-09 2001-01-23 Fuji Photo Film Co Ltd 蛍光診断装置
DE60109989T2 (de) * 2000-06-26 2006-02-23 Fuji Photo Film Co. Ltd., Minamiashigara Vorrichtung zur Aufnahme von Fluoreszenzbildern
JP4202671B2 (ja) 2001-04-27 2008-12-24 富士フイルム株式会社 規格化画像生成方法および装置
US7123756B2 (en) * 2001-04-27 2006-10-17 Fuji Photo Film Co., Ltd. Method and apparatus for standardized fluorescence image generation
JP2003164414A (ja) * 2001-11-29 2003-06-10 Fuji Photo Film Co Ltd 蛍光診断画像表示方法および表示装置
CN1169079C (zh) * 2002-03-25 2004-09-29 北京工业大学 基于样条思内克斯(Snakes)模型的中医舌象计算机分割方法
JP4311607B2 (ja) * 2002-05-27 2009-08-12 富士フイルム株式会社 蛍光診断情報生成方法および装置
EP1387315B1 (en) * 2002-06-07 2007-09-12 Honda Research Institute Europe GmbH Preparation of a digital image with subsequent edge detection
JP4199510B2 (ja) * 2002-09-30 2008-12-17 Hoya株式会社 診断補助用装置
JP2006175052A (ja) 2004-12-22 2006-07-06 Fuji Photo Film Co Ltd 蛍光画像撮像装置
US8129105B2 (en) * 2006-04-13 2012-03-06 Ralph Zuckerman Method and apparatus for the non-invasive measurement of tissue function and metabolism by determination of steady-state fluorescence anisotropy
CN100541601C (zh) * 2007-04-13 2009-09-16 四川长虹电器股份有限公司 亮度信号处理方法
JP5160343B2 (ja) * 2008-08-22 2013-03-13 オリンパスメディカルシステムズ株式会社 撮像システム及び内視鏡システム
JP5498481B2 (ja) * 2009-03-24 2014-05-21 オリンパス株式会社 蛍光観察装置、蛍光観察システム、蛍光観察装置の作動方法および蛍光観察装置が行う蛍光画像処理方法
JP5393216B2 (ja) * 2009-03-24 2014-01-22 オリンパス株式会社 蛍光観察システムおよび蛍光観察システムの作動方法
US8687859B2 (en) * 2009-10-14 2014-04-01 Carestream Health, Inc. Method for identifying a tooth region
JP5444377B2 (ja) * 2010-02-10 2014-03-19 オリンパス株式会社 蛍光内視鏡装置
CN102781305B (zh) * 2010-03-09 2015-06-03 奥林巴斯株式会社 荧光内窥镜装置
CN102802495B (zh) * 2010-03-18 2015-08-05 奥林巴斯株式会社 荧光观察装置以及荧光观察方法

Also Published As

Publication number Publication date
CN103764007A (zh) 2014-04-30
WO2013024773A1 (ja) 2013-02-21
US9182347B2 (en) 2015-11-10
EP2745761A4 (en) 2015-05-27
JP5816486B2 (ja) 2015-11-18
EP2745761B1 (en) 2018-10-10
JP2013039275A (ja) 2013-02-28
EP2745761A1 (en) 2014-06-25
US20140161369A1 (en) 2014-06-12

Similar Documents

Publication Publication Date Title
CN103764007B (zh) 荧光观察装置和荧光观察系统以及荧光图像处理方法
CN102361580B (zh) 荧光观察装置、荧光观察系统以及荧光图像处理方法
CN103687529B (zh) 荧光内窥镜装置
CN102355844B (zh) 荧光观察系统、荧光观察装置以及荧光观察方法
US9588046B2 (en) Fluorescence observation apparatus
CN102361581B (zh) 荧光观察装置、荧光观察系统以及荧光图像处理方法
CN102753079B (zh) 荧光内窥镜装置
CN102781305A (zh) 荧光内窥镜装置
CN102958417B (zh) 荧光观察装置
CN102802495B (zh) 荧光观察装置以及荧光观察方法
JP2009542415A (ja) 蛍光光画像の発生システムおよび方法
CN103764009A (zh) 荧光观察装置
CN105101861A (zh) 荧光观察装置
US9460496B2 (en) Fluorescence observation apparatus, fluorescence observation system, and method for fluorescence image processing
JP5592715B2 (ja) 画像処理装置および画像処理方法
JP5592700B2 (ja) 蛍光観察装置、蛍光観察システムおよび蛍光観察装置の作動方法

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20160413

Termination date: 20190808

CF01 Termination of patent right due to non-payment of annual fee