CN103739732B - A kind of method of extracting seed of hollyhock polysaccharide - Google Patents
A kind of method of extracting seed of hollyhock polysaccharide Download PDFInfo
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- CN103739732B CN103739732B CN201310735013.9A CN201310735013A CN103739732B CN 103739732 B CN103739732 B CN 103739732B CN 201310735013 A CN201310735013 A CN 201310735013A CN 103739732 B CN103739732 B CN 103739732B
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- 235000017334 Alcea rosea Nutrition 0.000 title claims abstract description 55
- 235000017303 Althaea rosea Nutrition 0.000 title claims abstract description 55
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 42
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 42
- 150000004676 glycans Chemical class 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 21
- 240000000530 Alcea rosea Species 0.000 title description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 56
- 241000921356 Alcea Species 0.000 claims abstract description 52
- 238000001556 precipitation Methods 0.000 claims abstract description 21
- 238000005238 degreasing Methods 0.000 claims abstract description 20
- 239000006286 aqueous extract Substances 0.000 claims abstract description 19
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims description 20
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 20
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 18
- 239000007788 liquid Substances 0.000 claims description 18
- 238000010992 reflux Methods 0.000 claims description 16
- 238000000605 extraction Methods 0.000 claims description 14
- 238000003809 water extraction Methods 0.000 claims description 10
- 238000002386 leaching Methods 0.000 claims description 9
- 238000000926 separation method Methods 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 12
- 239000000284 extract Substances 0.000 abstract description 11
- 238000011084 recovery Methods 0.000 abstract 1
- 239000000843 powder Substances 0.000 description 11
- 239000003153 chemical reaction reagent Substances 0.000 description 10
- 239000012141 concentrate Substances 0.000 description 10
- 238000001914 filtration Methods 0.000 description 10
- 239000006228 supernatant Substances 0.000 description 10
- 239000002904 solvent Substances 0.000 description 6
- GNFTZDOKVXKIBK-UHFFFAOYSA-N 3-(2-methoxyethoxy)benzohydrazide Chemical compound COCCOC1=CC=CC(C(=O)NN)=C1 GNFTZDOKVXKIBK-UHFFFAOYSA-N 0.000 description 5
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 5
- 238000001035 drying Methods 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 235000000346 sugar Nutrition 0.000 description 4
- MYKOKMFESWKQRX-UHFFFAOYSA-N 10h-anthracen-9-one;sulfuric acid Chemical compound OS(O)(=O)=O.C1=CC=C2C(=O)C3=CC=CC=C3CC2=C1 MYKOKMFESWKQRX-UHFFFAOYSA-N 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 239000012488 sample solution Substances 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 208000025865 Ulcer Diseases 0.000 description 2
- 150000001335 aliphatic alkanes Chemical class 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 231100000397 ulcer Toxicity 0.000 description 2
- 206010011224 Cough Diseases 0.000 description 1
- 235000007926 Craterellus fallax Nutrition 0.000 description 1
- 240000007175 Datura inoxia Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 208000000913 Kidney Calculi Diseases 0.000 description 1
- 241000219071 Malvaceae Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 206010029148 Nephrolithiasis Diseases 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
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- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 208000006568 Urinary Bladder Calculi Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000000202 analgesic effect Effects 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000000954 anitussive effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 229940124584 antitussives Drugs 0.000 description 1
- 210000000941 bile Anatomy 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- ZZVUWRFHKOJYTH-UHFFFAOYSA-N diphenhydramine Chemical compound C=1C=CC=CC=1C(OCCN(C)C)C1=CC=CC=C1 ZZVUWRFHKOJYTH-UHFFFAOYSA-N 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 201000001421 hyperglycemia Diseases 0.000 description 1
- 208000033065 inborn errors of immunity Diseases 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
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- 239000002245 particle Substances 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- -1 polysaccharide compound Chemical class 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 208000028529 primary immunodeficiency disease Diseases 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
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- Medicines Containing Plant Substances (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The present invention discloses a kind of method of extracting seed of hollyhock polysaccharide. The method comprises: 1) seed of hollyhock degreasing; 2) after degreasing, water extracts; 3) aqueous extract, through except alcohol precipitation after albumen, obtains seed of hollyhock polysaccharide. Adopt the inventive method can from seed of hollyhock, extract polysaccharide. The method is easy to operate, and gained purity of polysaccharide is high, and recovery rate is high.
Description
Technical field
Medicinal plants of the present invention provides field, is specifically related to a kind of method of extracting seed of hollyhock polysaccharide.
Background technology
Dimension medicine seed of hollyhock is that uighur medicine prescription ancestral blocks one of chief component medicine of wooden particle, is the seed of Malvaceae plant hollyhock (Althaearosea). Red, ripe season of one zhang of hollyhock another name flower, Rong Kui, Wu Kui, Wei Zukui, Hu Kui etc., Uygur's name is called in Acker. Hollyhock is perennial herb. Stem is upright and high. Leaf alternate, heart-shaped. Flower is the raw single-lobe in raceme top or raw lobe, has purple, powder, red, white isochrome. June at florescence is to August. Capsule, seed is oblate, kidney-shaped. Like sunnyly, resistance to half is cloudy, but avoid waterlogging. Originate in Sichuan Province China, distribution in China is very wide now, and East China, Central China, North China all have, and are distributed in the ground such as Europe, India, the former Soviet Union abroad. Its medicinal part is flower, root and seed. Dimension medicine seed of hollyhock head is loaded in Mo Hanmodeaikebaer Ai Erzhani and shows in " Ai Kebaer medical book ". Seed of hollyhock has multiple use as traditional Uygur's medicinal plant in Xinjiang, comprises that raw humidogene is cold, ripe abnormal bile matter, and clearing and antitussive, anti-inflammatory analgetic, gut purge is except ulcer, diuresis and stone expeling etc. For the xeothermic cough causing, enteron aisle ulcer, bladder and kidney stone.
Polysaccharide (polysaccharide) is the sugar chain by glycosidic bond combination, at least to exceed the polymerization sugar polymeric carbohydrate of 10 above monose compositions, polysaccharide is to be present in occurring in nature resource compared with the carbohydrate of horn of plenty, is extensively present in the organisms such as plant, animal and microorganism. Polysaccharide is macromolecular compound, on its sterling microcosmic, is inhomogenous, and usually said polysaccharide sterling is in fact the homogeneous components of certain molecular weight scope. It is extensively present in the cell membrane of animal cell membrane and plant, microorganism, and therefore bioactive polysaccharide mainly contains fungi polysaccharide, plant polyose, animal polysaccharide three major types. Polysaccharide is as a kind of natural polymer seed extract of almost non-toxic side effect, aspect the prevention and treatment of numerous disease, particularly some stubborn diseases, difficult disease, as aspects such as tumour, hypoimmunity, hyperglycaemia, aging, viral diseases, how the effect that has himself uniqueness, therefore, extract polysaccharide component from seed of hollyhock, allowing more polysaccharide compound for the mankind benefit, has been a good problem to study.
Summary of the invention
The object of this invention is to provide a kind of method of extracting seed of hollyhock polysaccharide.
It is as follows that the present invention realizes the technical scheme that above-mentioned purpose adopts:
A method of extracting seed of hollyhock polysaccharide, comprising:
1) seed of hollyhock degreasing;
2) after degreasing, water extracts;
3) aqueous extract, through except alcohol precipitation after albumen, obtains seed of hollyhock polysaccharide.
Further, described degreasing adopts solvent degreasing method, with leaching seed of hollyhocks such as the halogenated alkanes of C3-C10 alkane, C1-C3.
As leached seed of hollyhock with benzinum.
Further, when benzinum leaching seed of hollyhock, temperature is 40～80 DEG C.
When degreasing, repeatedly leach seed of hollyhock according to a small amount of principle repeatedly with benzinum, until petroleum ether layer is colourless.
Further, when water extraction is got, add hot reflux.
Further, the described albumen that removes can adopt protease solution or Sevage method, and wherein, Sevage method specifically contacts the mixed liquor of chloroform and n-butanol with aqueous extract, and vibration separates. Protein and chloroform
The volume ratio of described chloroform and n-butanol is (3～5): 1.
Further, when alcohol precipitation, the volumetric concentration of controlling alcohol in aqueous extract is 50%～95%.
Further, described alcohol is ethanol.
Further, water extraction is got detailed process and is: solid-liquid ratio is 1:41, then at 100 DEG C refluxing extraction 113 minutes, refluxing extraction 3 times; When alcohol precipitation, the volumetric concentration of controlling alcohol in aqueous extract is 60%. Under this condition, polysaccharide extract rate can reach 62.84mg/g.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further details.
Solid-liquid ratio described in the present invention refers to the ratio of quality (material) and volume (leaching liquor), the g/mL of unit.
Embodiment 1
A, seed of hollyhock is crushed to by 60 object sieves;
B, degreasing: press the solid-liquid ratio of 1:3, with benzinum leaching seed of hollyhock powder, in 60 DEG C of degreasings, continue half an hour, outwell supernatant (benzinum), again add benzinum repetition step last time until supernatant is colourless;
C, water extraction are got: will put into round-bottomed flask through step b seed of hollyhock powder after treatment, and add water, solid-liquid ratio is 1:50, then at 100 DEG C refluxing extraction 90 minutes, refluxing extraction 2 times;
D, filtration: with Buchner funnel and 1 layer of Filter paper filtering, separate residue and aqueous extract;
E, concentrated: aqueous extract is concentrated with Rotary Evaporators.
F, except albumen: in step e gained concentrate, add isopyknic Sevage reagent (chloroform: n-butanol=4:1, V/V), vibrate and pour separatory funnel into standing 20 minutes after 30 minutes, discard Sevage reagent layer;
G, alcohol precipitation: while stirring to adding ethanol through step f in except the concentrate after albumen, to the volumetric concentration of ethanol in solution be 60%, spend the night, carry out alcohol precipitation;
H, separating polyose: after step g alcohol precipitation, centrifugal, discard alcohol liquid;
I, dry: the centrifugal thing that h step is obtained is put into 60 DEG C of drying boxes and volatilized solvent, obtains seed of hollyhock polysaccharide, polysaccharide extract rate 40.68mg/g.
Embodiment 2
A, seed of hollyhock is crushed to by 60 object sieves;
B, degreasing: press the solid-liquid ratio of 1:3, with benzinum leaching seed of hollyhock powder, in 60 DEG C of degreasings, continue half an hour, outwell supernatant (benzinum), again add benzinum repetition step last time until supernatant is colourless;
C, water extraction are got: will put into round-bottomed flask through step b seed of hollyhock powder after treatment, and add water, solid-liquid ratio is 1:40, then at 100 DEG C refluxing extraction 30 minutes, refluxing extraction 3 times;
D, filtration: with Buchner funnel and 1 layer of Filter paper filtering, separate residue and aqueous extract;
E, concentrated: aqueous extract is concentrated with Rotary Evaporators.
F, except albumen: in step e gained concentrate, add isopyknic Sevage reagent (chloroform: n-butanol=4:1, V/V), vibrate and pour separatory funnel into standing 20 minutes after 30 minutes, discard Sevage reagent layer;
G, alcohol precipitation: while stirring to adding ethanol through step f in except the concentrate after albumen, to the volumetric concentration of ethanol in solution be 90%, spend the night, carry out alcohol precipitation;
H, separating polyose: after step g alcohol precipitation, centrifugal, discard alcohol liquid;
I, dry: the centrifugal thing that h step is obtained is put into 60 DEG C of drying boxes and volatilized solvent, obtains seed of hollyhock polysaccharide, polysaccharide extract rate 32.76mg/g.
Embodiment 3
A, seed of hollyhock is crushed to by 60 object sieves;
B, degreasing: press the solid-liquid ratio of 1:3, with benzinum leaching seed of hollyhock powder, in 60 DEG C of degreasings, continue half an hour, outwell supernatant (benzinum), again add benzinum repetition step last time until supernatant is colourless;
C, water extraction are got: will put into round-bottomed flask through step b seed of hollyhock powder after treatment, and add water, solid-liquid ratio is 1:40, then at 100 DEG C refluxing extraction 90 minutes, refluxing extraction 1 time;
D, filtration: with Buchner funnel and 1 layer of Filter paper filtering, separate residue and aqueous extract;
E, concentrated: aqueous extract is concentrated with Rotary Evaporators.
F, except albumen: in step e gained concentrate, add isopyknic Sevage reagent (chloroform: n-butanol=4:1, V/V), vibrate and pour separatory funnel into standing 20 minutes after 30 minutes, discard Sevage reagent layer;
G, alcohol precipitation: while stirring to adding ethanol through step f in except the concentrate after albumen, to the volumetric concentration of ethanol in solution be 80%, spend the night, carry out alcohol precipitation;
H, separating polyose: after step g alcohol precipitation, centrifugal, discard alcohol liquid;
I, dry: the centrifugal thing that h step is obtained is put into 60 DEG C of drying boxes and volatilized solvent, obtains seed of hollyhock polysaccharide, polysaccharide extract rate 37.80mg/g.
Embodiment 4
A, seed of hollyhock is crushed to by 60 object sieves;
B, degreasing: press the solid-liquid ratio of 1:3, with benzinum leaching seed of hollyhock powder, in 60 DEG C of degreasings, continue half an hour, outwell supernatant (benzinum), again add benzinum repetition step last time until supernatant is colourless;
C, water extraction are got: will put into round-bottomed flask through step b seed of hollyhock powder after treatment, and add water, solid-liquid ratio is 1:20, then at 100 DEG C refluxing extraction 90 minutes, refluxing extraction 3 times;
D, filtration: with Buchner funnel and 1 layer of Filter paper filtering, separate residue and aqueous extract;
E, concentrated: aqueous extract is concentrated with Rotary Evaporators.
F, except albumen: in step e gained concentrate, add isopyknic Sevage reagent (chloroform: n-butanol=4:1, V/V), vibrate and pour separatory funnel into standing 20 minutes after 30 minutes, discard Sevage reagent layer;
G, alcohol precipitation: while stirring to adding ethanol through step f in except the concentrate after albumen, to the volumetric concentration of ethanol in solution be 70%, spend the night, carry out alcohol precipitation;
H, separating polyose: after step g alcohol precipitation, centrifugal, discard alcohol liquid;
I, dry: the centrifugal thing that h step is obtained is put into 60 DEG C of drying boxes and volatilized solvent, obtains seed of hollyhock polysaccharide, polysaccharide extract rate 33.66mg/g.
Embodiment 5
A, seed of hollyhock is crushed to by 60 object sieves;
B, degreasing: press the solid-liquid ratio of 1:3, with benzinum leaching seed of hollyhock powder, in 60 DEG C of degreasings, continue half an hour, outwell supernatant (benzinum), again add benzinum repetition step last time until supernatant is colourless;
C, water extraction are got: will put into round-bottomed flask through step b seed of hollyhock powder after treatment, and add water, solid-liquid ratio is 1:41, then at 100 DEG C refluxing extraction 113 minutes, refluxing extraction 3 times;
D, filtration: with Buchner funnel and 1 layer of Filter paper filtering, separate residue and aqueous extract;
E, concentrated: aqueous extract is concentrated with Rotary Evaporators.
F, except albumen: in step e gained concentrate, add isopyknic Sevage reagent (chloroform: n-butanol=4:1, V/V), vibrate and pour separatory funnel into standing 20 minutes after 30 minutes, discard Sevage reagent layer;
G, alcohol precipitation: while stirring to adding ethanol through step f in except the concentrate after albumen, to the volumetric concentration of ethanol in solution be 60%, spend the night, carry out alcohol precipitation;
H, separating polyose: after step g alcohol precipitation, centrifugal, discard alcohol liquid;
I, dry: the centrifugal thing that h step is obtained is put into 60 DEG C of drying boxes and volatilized solvent, obtains seed of hollyhock polysaccharide, polysaccharide extract rate 62.84mg/g.
In the present invention, polysaccharide adopts sulfuric acid-anthrone method to measure, and concrete steps are as follows:
1, standard curve making: precision takes glucose sugar 0.1000g and puts in 100ml volumetric flask, is settled to scale with distilled water, obtains glucose Standard for Sugars solution; Accurate draw 0.2,0.4,0.6,0.8,1.0,1.2mL, be placed in respectively 10mL volumetric flask, be settled to scale with distilled water; Get respectively again 1mL and be placed in test tube, respectively add sulfuric acid-anthrone solution 4mL, shake up rear placement 10min, put in boiling water and heat 10min, take out and be cooled to room temperature (15min), measure absorbance in 582nm place, drawing standard curve, obtains the curve of glucose solution concentration (c) and absorbance (A).
2, the seed of hollyhock polysaccharide distilled water obtaining is settled to 200mL, gets 5mL and be diluted to 10mL, be i.e. sample solution; The sample solution of drawing 1mL is placed in test tube, adds sulfuric acid-anthrone solution 4mL, shakes up rear placement 10min, puts in boiling water and heats 10min, takes out and is cooled to room temperature (15min), measures absorbance in 582nm place. Bring calibration curve equation into, obtain the concentration of polysaccharide in sample solution, and then calculate its content.
Claims (6)
1. a method of extracting seed of hollyhock polysaccharide, is characterized in that, comprising:
1) seed of hollyhock degreasing;
2) the water extraction again of the seed of hollyhock after degreasing, obtains aqueous extract;
3) aqueous extract, through except alcohol precipitation after albumen, obtains seed of hollyhock polysaccharide;
Water extraction is got detailed process: solid-liquid ratio is 1:41, then at 100 DEG C refluxing extraction 113 minutes, refluxing extraction 3 times;
When alcohol precipitation, the volumetric concentration of controlling ethanol in aqueous extract is 60%, and described alcohol is ethanol.
2. the method for extracting according to claim 1 seed of hollyhock polysaccharide, is characterized in that, described degreasing is to leach seed of hollyhock with benzinum.
3. the method for extracting according to claim 2 seed of hollyhock polysaccharide, is characterized in that, when benzinum leaching seed of hollyhock, temperature is 40～80 DEG C.
4. the method for extracting according to claim 1 seed of hollyhock polysaccharide, is characterized in that, when water extraction is got, adds hot reflux.
5. the method for extracting according to claim 1 seed of hollyhock polysaccharide, is characterized in that, described except albumen be that the mixed liquor of chloroform and n-butanol contact with aqueous extract, vibrate, separation.
6. the method for extracting according to claim 5 seed of hollyhock polysaccharide, is characterized in that, the volume ratio of described chloroform and n-butanol is (3～5): 1.
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| CN103976466B (en) * | 2014-05-20 | 2016-06-01 | 河南中烟工业有限责任公司 | The application of yellow hollyhock seed extract in cigarette |
| CN104082846B (en) * | 2014-07-24 | 2016-02-24 | 中国烟草总公司郑州烟草研究院 | The application of a kind of hollyhock seed extract in cigarette |
| CN104087422B (en) * | 2014-07-24 | 2016-03-16 | 中国烟草总公司郑州烟草研究院 | A kind of preparation method of hollyhock rhizome Aqueous extracts and application thereof |
| CN106008729B (en) * | 2016-04-14 | 2018-05-25 | 南京中医药大学 | A kind of sunset abelmoschus stem or bark leaf polyose and preparation method and application |
| CN105859906B (en) * | 2016-06-12 | 2018-07-13 | 南京中医药大学 | With immunocompetent sunset abelmoschus stem or bark leaf polyose acetylation modification product of raising and preparation method thereof |
| CN105859907B (en) * | 2016-06-12 | 2018-06-19 | 南京中医药大学 | With immunocompetent sunset abelmoschus stem or bark leaf polyose sulphation modification product of raising and preparation method thereof |
| CN106046187B (en) * | 2016-06-28 | 2018-06-19 | 南京中医药大学 | With free radical cracking product for improving immunocompetent sunset abelmoschus stem or bark leaf polyose and preparation method thereof |
| CN105968221B (en) * | 2016-06-28 | 2018-08-14 | 南京中医药大学 | With the acid system catabolite and preparation method thereof for improving immunocompetent sunset abelmoschus stem or bark leaf polyose |
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| CN103087213A (en) * | 2013-02-01 | 2013-05-08 | 李育材 | Preparation method of polysaccharide of tree peony seeds |
| CN103435711B (en) * | 2013-07-17 | 2016-05-18 | 新乡医学院 | A kind of extracting method of black ugly polysaccharide |
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