CN104523926B - A kind of preparation method of lemon extract, its product and application - Google Patents
A kind of preparation method of lemon extract, its product and application Download PDFInfo
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- CN104523926B CN104523926B CN201410798707.1A CN201410798707A CN104523926B CN 104523926 B CN104523926 B CN 104523926B CN 201410798707 A CN201410798707 A CN 201410798707A CN 104523926 B CN104523926 B CN 104523926B
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Abstract
The invention discloses a kind of preparation method of lemon extract, its product and application.The preparation method comprises the following steps：(1) it will remove the peel, except the Fresh Lemon after seed squeezes broken, and separate solid phase and liquid phase, obtain the lemon fluid and lemon muddy flesh of clarification；(2) obtained lemon fluid in step (1) is subjected to frozen dried, water-soluble substances lyophilized products is made；The lemon muddy flesh obtained in step (1) is subjected to frozen dried, powder is ground into, is then extracted with ethyl acetate between 8 DEG C to 2 DEG C, the liposoluble substance after extraction is removed into solvent, liposoluble substance powder is made；(3) by obtained water-soluble substances lyophilized products and liposoluble substance powder in step (2).The lemon extract prepared according to methods described, is uric acid health food or medicine applied to preparing, works well, while having certain protective effect to renal function.
Description
Technical field
The invention belongs to biomedicine field, more particularly, to a kind of preparation method of lemon extract, its product and
Using.
Background technology
Uric acid is the final product of purine catabolism in nucleic acid, mainly by kidney excretion.Uric acid generation increases or arranged
Reduction is let out, uric acid can be made to accumulate and cause hyperuricemia.
With the improvement of living standards, the change and aging population of life style and dietary structure, China's antihyperuricemic
Disease morbidity is in gradually rejuvenation trend, and patient groups ramp.Numerous studies show that hyperuricemia can not only cause gout
Property arthritis, lithangiuria, renal dysfunction etc., and with hypertension, left ventricular hypertrophy, insulin resistance, obesity, hyperlipemia
Disease, IGT etc. have close relationship, be aggravate atherosclerosis, promote Cardial or cerebral vascular diseases occur it is important because
Element.
At present, drug therapy is substantially carried out for hyperuricemia, corresponding medicine is broadly divided into three major types：(1) suppress
The medicine of uric acid synthesis, mainly there is allopurinol and Febuxostat.(2) promote uric acid excretion medicine, mainly have probenecid and
Benzbromarone.(3) promote uricolytic medicine, mainly there is rasburicase and polyethylene glycol uricase.These medicines are all to close
Patent medicine, long-term taking side effect is substantially, serious to liver and kidney dysfunction, and patient compliance is poor.Therefore, need badly and develop newly
The medicine of natural treatment hyperuricemia, it can reduce uric acid and patient is had no toxic side effect.
The content of the invention
For the disadvantages described above or Improvement requirement of prior art, the invention provides a kind of preparation side of lemon extract
Method, its product and application, its object is to by extracting water-soluble freeze-dried powder and liposoluble substance powder in lemon, and by two
Person mixes according to a certain percentage, a kind of composition acted on anti-trioxypurine is developed, for preparing anti-trioxypurine health food or medicine
Thing, thus solves the existing synthesis obvious technical problem of anti-trioxypurine medicine long-term taking side effect.
To achieve the above object, according to one aspect of the present invention, there is provided a kind of preparation method of lemon extract, bag
Include following steps：
(1) it will remove the peel, except the Fresh Lemon after seed squeezes broken, and separate solid phase and liquid phase, obtain the lemon fluid and lemon of clarification
Lemon muddy flesh；
(2) obtained lemon fluid in step (1) is subjected to frozen dried, water-soluble substances lyophilized products is made；By step (1)
The lemon muddy flesh of middle acquisition carries out frozen dried, is ground into powder, is then extracted with ethyl acetate between -8 DEG C to -2 DEG C
Take, the liposoluble substance after extraction is removed into solvent, liposoluble substance powder is made；
(3) by obtained water-soluble substances lyophilized products and liposoluble substance powder in step (2) according to mass ratio 11:1
To 8:1 is mixed, that is, the lemon extract is made.
Preferably, the preparation method of the lemon extract, the lemon fluid its preparation method described in its step (1) is specific such as
Under：
By for peeling, except the Fresh Lemon after seed squeeze it is broken after, the liquid through gauze leaching, then the liquid is centrifuged, its
Supernatant is the lemon fluid.
Preferably, the preparation method of the lemon extract, the Lemon fruit muddy flesh its preparation method tool described in its step (1)
Body is as follows：
By for peeling, except the Fresh Lemon after seed squeeze it is broken after, the liquid through gauze leaching, and retain solid, then will be described
Liquid is centrifuged, and it is the Lemon fruit muddy flesh that it, which is deposited in the solid to merge,.
Preferably, the preparation method of the lemon extract, its step (3) water-soluble substances lyophilized products and liposoluble substance
Powder is according to mass ratio 10:1 mixing.
It is another aspect of this invention to provide that there is provided a kind of lemon extract, being prepared according to described preparation method.
It is another aspect of this invention to provide that there is provided a kind of application of the lemon extract, it is applied to prepare drop urine
Sour health food or medicine.
It is another aspect of this invention to provide that there is provided a kind of anti-trioxypurine composition, containing described lemon extract.
In general, by the contemplated above technical scheme of the present invention compared with prior art, it can obtain down and show
Beneficial effect：
The preparation method for the lemon extract that the present invention is provided, it is simple for process, it is adapted to large-scale production.The lemon
Extract, retains the natural active material of lemon, composition safety and Health, with obvious anti-trioxypurine effect, while to renal function
With certain protective effect.The drop prepared relative to existing anti-trioxypurine medicine, the lemon extract provided using the present invention
Uric acid health food or medicine, drug effect are clearly and warm, without side effects such as liver renal toxicitys, further have one to renal function
Fixed protective effect, therefore with good application prospect and market prospects.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.As long as in addition, technical characteristic involved in each embodiment of invention described below each other it
Between do not constitute conflict can just be mutually combined.
The preparation method for the lemon extract that the present invention is provided, comprises the following steps：
(1) it will remove the peel, except the Fresh Lemon after seed squeezes broken, and separate solid phase and liquid phase, obtain the lemon fluid and lemon of clarification
Lemon muddy flesh, its specific method is as follows：
Will remove the peel, except after seed Fresh Lemon squeeze it is broken after, the liquid through gauze leaching, it is preferred to use three layers of filtered through gauze 2
It is secondary, then filtrate is centrifuged, the supernatant after centrifugation is the lemon fluid, and the precipitation being deposited in after filtering after centrifugation merges
I.e. described lemon muddy flesh.
(2) obtained lemon fluid in step (1) is subjected to frozen dried, water-soluble substances lyophilized products is made；By step (1)
The lemon muddy flesh of middle acquisition carries out frozen dried, is ground into powder, is then extracted with ethyl acetate at -8 DEG C to -2 DEG C, will
Liposoluble substance after extraction removes solvent, and liposoluble substance powder is made.
Temperature is preferably -4 DEG C or so, because ethyl acetate is inflammable, and temperature crosses high-risk, and too low effect is poor, so extraction
Operational temperature conditions are -8 DEG C to -2 DEG C, optimal at -4 DEG C.Ethyl acetate is volatile as fat-soluble extractant, and effect is good, together
When nitrogen drying technology fully ethyl acetate can be removed, the volatile liposoluble substance in lemon be not belonging to important function into
Point, it is to avoid loss.
(3) by obtained water-soluble substances lyophilized products and liposoluble substance powder in step (2) according to mass ratio 11:1
To 8:1 is mixed, and preferably 10:1, that is, the lemon extract is made.
Sodium carboxymethylcellulose plays a part of to reconcile, allows fat-soluble and water-soluble be preferably blended in one as thickener
Rise.Adding proportion 0.5%~1%, preferably 0.5%.
The method provided according to the present invention, the lemon extract of preparation, for anti-trioxypurine animal model.Pass through animal model
Contrast test is determined, and anti-trioxypurine lemon extract is compared with antihyperuricemic disease mouse model group in the present invention：Uric Acid Content is put down
It has dropped in more than 21.0%, blood creatinine content and decline urea nitrogen in 30.1%, blood and decline up to 8.4%.To sum up show,
Lemon extract has significant anti-trioxypurine effect, and has certain protective effect to kidney, available for preparing uric acid
Medicine or health food.
Invention also provides a kind of anti-trioxypurine medicine, using the lemon extract of the invention provided as active component.
It is embodiment below：
Embodiment 1
The preparation method for the lemon extract that the present invention is provided, comprises the following steps：
(1) it will remove the peel, except the Fresh Lemon after seed squeezes broken, and separate solid phase and liquid phase, obtain the lemon fluid and lemon of clarification
Lemon muddy flesh, its specific method is as follows：
By fresh You Like lemons peeling, except being cut into small pieces after seed, it is fitted into three times in juice extractor and squeezes 10 minutes, institute
Lemon composition with 3 layers of filtered through gauze twice, centrifuged to filtrate, under conditions of 5000rpm centrifugation 15 minutes, centrifuge
Supernatant afterwards is the lemon fluid, and the precipitation being deposited in after filtering after centrifugation merges i.e. described lemon muddy flesh.
(2) drying solid is sticked together for yellow bulk after obtained lemon fluid in step (1) is freezed in freeze drier,
Water-soluble substances lyophilized products are made；The lemon muddy flesh that will be obtained in step (1), blows out powder with nitrogen, then uses ethyl acetate
Extracted at -4 DEG C, per 1mg powder addition 30ml ethyl acetate, after after ethyl acetate layer flavescence, draw ethyl acetate layer, will
The ethyl acetate layer of acquisition removes ethyl acetate solvent with nitrogen drying instrument drying, and liposoluble substance powder is made.
(3) obtained water-soluble substances lyophilized products 15g and liposoluble substance powder 1.5g is taken in step (2) in mortar,
Its mass ratio 10:1, sodium carboxymethylcellulose 0.5g is added, a small amount of distilled water ground and mixed is added, that is, the lemon is made
Extract.
According to lemon extract made from this method, 100ml is settled to, the volumetric flask after constant volume is put into Ultrasound Instrument and surpassed
Sound 10min, then be transferred in centrifuge tube and obtain the lemon extract aqueous solution i.e. low dosage solution of extract.It is another to take out the aqueous solution
60ml is concentrated under reduced pressure into 30ml, that is, obtains high dose solution of extract.
Embodiment 2
(1) it will remove the peel, except the Fresh Lemon after seed squeezes broken, and separate solid phase and liquid phase, obtain the lemon fluid and lemon of clarification
Lemon muddy flesh, its specific method is as follows：
By fresh You Like lemons peeling, except being cut into small pieces after seed, it is fitted into three times in juice extractor and squeezes 10 minutes, institute
Lemon composition with 3 layers of filtered through gauze twice, centrifuged to filtrate, under conditions of 5000rpm centrifugation 15 minutes, centrifuge
Supernatant afterwards is the lemon fluid, and the precipitation being deposited in after filtering after centrifugation merges i.e. described lemon muddy flesh.
(2) drying solid is sticked together for yellow bulk after obtained lemon fluid in step (1) is freezed in freeze drier,
Water-soluble substances lyophilized products are made；The lemon muddy flesh that will be obtained in step (1), powder is blown out with nitrogen, then with acetoacetic ester-
2 DEG C are extracted, and per 1mg powder addition 30ml ethyl acetate, after after ethyl acetate layer flavescence, are drawn ethyl acetate layer, will be obtained
The ethyl acetate layer obtained removes ethyl acetate solvent with nitrogen drying instrument drying, and liposoluble substance powder is made.
(3) obtained water-soluble substances lyophilized products 16g and liposoluble substance powder 2g is taken in step (2) in mortar, its
Mass ratio 8:1, sodium carboxymethylcellulose 0.5g is added, a small amount of distilled water ground and mixed is added, that is, the lemon is made and carries
Take thing.
According to lemon extract made from this method, 100ml is settled to, the volumetric flask after constant volume is put into Ultrasound Instrument and surpassed
Sound 10min, then be transferred in centrifuge tube and obtain the lemon extract aqueous solution i.e. low dosage solution of extract.It is another to take out the aqueous solution
60ml is concentrated under reduced pressure into 30ml, that is, obtains high dose solution of extract.
Embodiment 3
(1) it will remove the peel, except the Fresh Lemon after seed squeezes broken, and separate solid phase and liquid phase, obtain the lemon fluid and lemon of clarification
Lemon muddy flesh, its specific method is as follows：
By fresh You Like lemons peeling, except being cut into small pieces after seed, it is fitted into three times in juice extractor and squeezes 10 minutes, institute
Lemon composition with 3 layers of filtered through gauze twice, centrifuged to filtrate, under conditions of 5000rpm centrifugation 15 minutes, centrifuge
Supernatant afterwards is the lemon fluid, and the precipitation being deposited in after filtering after centrifugation merges i.e. described lemon muddy flesh.
(2) drying solid is sticked together for yellow bulk after obtained lemon fluid in step (1) is freezed in freeze drier,
Water-soluble substances lyophilized products are made；The lemon muddy flesh that will be obtained in step (1), blows out powder with nitrogen, then uses ethyl acetate
Extracted at -8 DEG C, per 1mg powder addition 30ml ethyl acetate, after after ethyl acetate layer flavescence, draw ethyl acetate layer, will
The ethyl acetate layer of acquisition removes ethyl acetate solvent with nitrogen drying instrument drying, and liposoluble substance powder is made.
(3) obtained water-soluble substances lyophilized products 22g and liposoluble substance powder 2g is taken in step (2) in mortar, its
Mass ratio 11:1, sodium carboxymethylcellulose 0.5g is added, a small amount of distilled water ground and mixed is added, that is, the lemon is made and carries
Take thing.
According to lemon extract made from this method, 100ml is settled to, the volumetric flask after constant volume is put into Ultrasound Instrument and surpassed
Sound 10min, then be transferred in centrifuge tube and obtain the lemon extract aqueous solution i.e. low dosage solution of extract.It is another to take out the aqueous solution
60ml is concentrated under reduced pressure into 30ml, that is, obtains high dose solution of extract.
Embodiment 4
The healthy cleaning grade male mice in kunming 50 (Hubei Province Animal Experimental Study center) of 18~22g of body weight are taken, divide 5
Group, respectively every group 10, normal group, hyperuricemia model group, positive controls (Allopurinol Tablets), high dose test group
With low dosage test group.In addition to normal group, the equal gavage 250mg/kg of remaining each group Oteracil Potassium, the distillation of normal group equivalent
Water.After one hour, positive controls gavage 5mg/kg allopurinol, high dose test group and low dosage test group distinguish gavage
The high dose solution of extract and low dosage solution of extract of equivalent, the steaming of normal group and high lithemia model group gavage equivalent
Distilled water.7 days experimental periods, pluck eyeball and take blood in centrifuge tube, centrifuge 10min under the conditions of 3000r, 3 DEG C, take serum, use blood
Fully-automatic analyzer surveys blood uric acid, creatinine and urea nitrogen content, then takes out liver and kidney observation respectively.Obtain knot shown in table 1
Really.
Influence of the lemon of table 1 to mouse uric acid, creatinine and urea nitrogen
As shown in table 1, the lemon extract in embodiment 1 to 3, all there is obvious anti-trioxypurine effect, and wherein high dose drops
Uric acid lemon extract can be effectively reduced the content of blood uric acid, and high dose test group is more obvious than low dosage test group effect, especially
It is the high dose group of embodiment 1, and its Uric Acid Content averagely have dropped creatinine content in more than 21.0%, blood and decline
30.1%, urea nitrogen declines up to 8.4% in blood.Meanwhile, but lemon extract does not have any side effect, also has to renal function
There is certain protective effect, so the extract has very high Development volue to treatment hyperuricemia.
Embodiment 5
By obtained lemon extract in embodiment 1 to 3, superfine silica gel powder and cellulose are added, is well mixed, extruding is cut
Block, that is, obtain anti-trioxypurine health food, i.e. sheet dietary supplements.
As it will be easily appreciated by one skilled in the art that the foregoing is merely illustrative of the preferred embodiments of the present invention, it is not used to
The limitation present invention, any modifications, equivalent substitutions and improvements made within the spirit and principles of the invention etc., it all should include
Within protection scope of the present invention.
Claims (5)
1. the preparation method of a kind of lemon extract for anti-trioxypurine, it is characterised in that comprise the following steps：
(1) it will remove the peel, except the Fresh Lemon after seed squeezes broken, and separate solid phase and liquid phase, obtain the lemon fluid and lemon meat of clarification
Mud；
(2) obtained lemon fluid in step (1) is subjected to frozen dried, water-soluble substances lyophilized products is made；It will be obtained in step (1)
The lemon muddy flesh obtained carries out frozen dried, is ground into powder, is then extracted with ethyl acetate between -8 DEG C to -2 DEG C, will
Liposoluble substance after extraction removes solvent, and liposoluble substance powder is made；
(3) by obtained water-soluble substances lyophilized products and liposoluble substance powder in step (2) according to mass ratio 11:1 to 8:1
Mixed, add thickener and water, ground and mixed is made the lemon extract；
Wherein, the lemon fluid its preparation method described in step (1) is specific as follows：
To remove the peel, except the Fresh Lemon after seed squeeze it is broken after, the liquid through gauze leaching, then the liquid is centrifuged, its supernatant
As described lemon fluid；
Lemon fruit muddy flesh its preparation method described in step (1) is specific as follows：
To remove the peel, except the Fresh Lemon after seed squeeze it is broken after, the liquid through gauze leaching, and retain solid, then by the liquid from
The heart, its precipitation merges with the solid Ji Wei the Lemon fruit muddy flesh.
2. the preparation method of lemon extract as claimed in claim 1, it is characterised in that step (3) water-soluble substances
Lyophilized products and liposoluble substance powder are according to mass ratio 10:1 mixing.
3. a kind of lemon extract, it is characterised in that prepared according to preparation method as claimed in claim 1 or 2.
4. a kind of application of lemon extract as claimed in claim 3, it is applied to prepare anti-trioxypurine health food or medicine.
5. a kind of anti-trioxypurine composition, it is characterised in that contain lemon extract as claimed in claim 3.
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| CN108865473A (en) * | 2018-06-27 | 2018-11-23 | 合肥同佑电子科技有限公司 | A kind of scale remover and preparation method thereof for computor-keyboard |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101731402A (en) * | 2009-12-25 | 2010-06-16 | 黄顺邦 | Processing process of lemon jelly dried molassed tablets |
| CN102603697A (en) * | 2012-02-06 | 2012-07-25 | 四川亿乙显生物科技有限公司 | Process for extracting polymethoxylated flavones from lemon |
-
2014
- 2014-12-19 CN CN201410798707.1A patent/CN104523926B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101731402A (en) * | 2009-12-25 | 2010-06-16 | 黄顺邦 | Processing process of lemon jelly dried molassed tablets |
| CN102603697A (en) * | 2012-02-06 | 2012-07-25 | 四川亿乙显生物科技有限公司 | Process for extracting polymethoxylated flavones from lemon |
Non-Patent Citations (2)
| Title |
|---|
| 原料预处理对柠檬膳食纤维总黄酮的影响;贺金梅;《食品工程》;20101231(第4期);第34-38页 * |
| 柠檬加工利用研究;程劲;《四川食品与发酵》;20051231(第1期);第11-15页 * |
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