—=—5~ —- — - —- ——. 1358261 —明說明： —. · —_ -— —===~ 【發明所屬之技術領域】 本發明係、有關於-種萃取洛神花花#素之方法，尤其是一種 能提高花青素之萃取量且*含有毒溶劑的提高萃取洛神花花青素 含量之方法。 ' 【先前技術】 洛神花為-年生木質狀草本植物，於洛神花肥厚的花等中， 3有機^、㈣、果膠及多紛類，而多紛類中的花青素係為強有 力的抗氧化物，其具有預防心血管疾病、防止膠原纖維及彈性纖 維的退化，並加強腦内抗氧化等功能。 、一般萃取洛神花中的花青素之方法，通㈣將乾燥的洛神花 以熱水煮彿飲用’然而，此方法萃取屮的^主主心 左早取出的化青素較少，因此.有研 究指出將洛神Μ乙醇浸泡至溶出色純，將浸泡過洛神花的乙 醇乾燥後’得到含有洛神花色素之粉末，將含有洛料色素之粉 末浸泡於已調酸的曱醇中，妨罟私含 、， 邶甲放置於至溫-天的時間予以沉澱，取 上清液加入乙並放置於4°c下，；| 士么 L下於12小時後收集沉澱物，該沉 運又物即為洛神花花青素。（王倍民，，λ λ λ 4一 & 2000。洛神花色素成分及其 抗氧化活性之分析，私立中山醫輋 褚干h生物化學研究所，碩士論文 。）雖然此法相較於水煮法可萃取 山平乂夕的化責素，但萃取過程 中所使用的曱醇及乙趟等溶劍皆且古 。+ 一 #有骨性’因此不適合使用於食 〇〇中 0 【發明内容】 秦包=括4減壓•的私除去·的‘秦乙酯二— 以減壓濃縮的 其中’水煮師花獲取該洛神花濃縮液係包括 方式獲取該洛神花濃縮液。 其中，將該去多醣之洛神花濃縮液乾燥，係包括將該去多聽 之洛神花濃縮灰進行冷珠乾燥。 -中洛神:f匕的種類為紐心“"以如咖l。 藉由洛神花濃縮液經過乙酸以旨進行萃取，並湘乙醇去除—=—5~ — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — The method of the method, in particular, a method for increasing the extraction amount of anthocyanin and containing a toxic solvent to increase the content of anthocyanin in the extract. '[Previous technique] Luoshenhua is an annual woody herbaceous plant, in the rich flowers of Luoshenhua, 3 organic ^, (4), pectin and many other types, and the anthocyanins in many species are powerful It is an antioxidant that prevents cardiovascular disease, prevents the degradation of collagen fibers and elastic fibers, and strengthens antioxidant functions in the brain. In general, the method of extracting anthocyanins from Luoshenhua is used. (4) The dried Luoshen flowers are boiled in hot water for drinking. However, this method extracts the main body of the sputum. Some studies have pointed out that the soaking of ethanol is soaked to the pure and pure, and the ethanol soaked in the lotus flower is dried to obtain a powder containing the lotus pigment, and the powder containing the pigment of the Luo is soaked in the acidified sterol. Privately, the armor is placed in the temperature-to-day time to be precipitated, and the supernatant is added to the B and placed at 4 ° C;; | The L. collects the precipitate after 12 hours, and the sediment is collected. It is the flower anthocyanin. (Wang Beimin,, λ λ λ 4 & 2000. Analysis of anthocyanin constituents and antioxidant activities of the Luoshen, private Zhongshan Institute of Biochemistry, Master's thesis.) Although this method is compared to boiled water The method can extract the chemistry of the mountain ridge, but the sulphur and acetamone used in the extraction process are ancient. + 一#有骨性' is therefore not suitable for use in restaurants. [Invention content] Qin Bao = 4 decompression • private removal · 'Qin Ethyl Ethyl-- Obtaining the Luoshen flower concentrate system includes obtaining the Luoshen flower concentrate. Wherein, drying the de-polysaccharide Luoshen flower concentrate comprises cooling the dried ash of the scented scent of the scent of the scent of the scent. - Zhongluo God: The type of f匕 is the heart of the "", such as the coffee l. By the Luoshen flower concentrate through acetic acid for extraction, and ethanol removal
’所㈣的總花青素含量有所提升，且本發明所使用的 浴劑皆不具毒性，因此提高在製造過程或使用時的安全性。 【實施方式】 請參看第-圖所示，本發明提高萃取洛神花花青素含量之方 法’其係以下述方法所製備’其包括水煮步驟⑷、萃取步驟⑻ 、去多醣步驟（c)以及乾燥步驟（d): 本發明之洛神花的種類為·心…·伽l，先將洛 #神花加人沸騰的水並進行減壓濃縮以獲取—洛神花濃縮液，由於 乙酸乙自旨可與該洛神花濃縮液中的部分多酚類化合物互溶，因此 將該洛神花濃縮独乙酸乙料取，可將溶於乙酸乙㈣多紛類 化合物移除’以獲取一已萃取之洛神花濃縮液，而多畴類於高濃 度的醇類中溶解度較低，因此加人乙醇於該已萃取之洛神花濃縮 液’可使不溶於乙醇的多醣類析出’藉由抽氣賴的方式即可去 除多餹類’將—去多醣之洛神花濃縮液進行減壓濃縮及冷來乾燥 等步驟處理後，即可獲取—含花青素之粉末，且能提高萃取洛神 it*花青素含量。 n ·:'將實施例1所述之洛神花溶液直接進行乾燥步驟， 「得到含花青素之粉末。 .. …、萃取法.將實施例1所述之已萃取之洛神花濃縮液直 接進行㈣㈣，即_含Μ素之粉末。 曲 …、萃取人去夕醣法：將實施例丨所述之去多醣之洛神花 _«彳f乾燥步驟’即得到含花青素之粉末。 芝法所得之總花青素含量比較表:The total anthocyanin content of (4) is improved, and the bath used in the present invention is not toxic, thereby improving the safety during the manufacturing process or use. [Embodiment] Referring to Figure - Figure, the method for improving the content of anthocyanin extracted from the invention is prepared by the following method, which comprises a boiling step (4), an extraction step (8), a de-polysaccharide step (c), and Drying step (d): The type of Luoshen flower of the present invention is · heart...·g1, first add Luo# Shenhua to boiling water and concentrate under reduced pressure to obtain - Luoshen concentrated liquid, due to the purpose of acetic acid It can be miscible with some polyphenolic compounds in the Luoshen flower concentrate, so the concentrated scent of the scented scented scented acetic acid can be removed, and the compound dissolved in the ethyl acetate (four) can be removed to obtain an extracted lotus flower. Concentrate, and multi-domains have low solubility in high-concentration alcohols, so adding ethanol to the extracted Luoshen concentrate can precipitate ethanol-insoluble polysaccharides by means of pumping The polyterpenoids can be removed, and the polysaccharides of the polysaccharides can be obtained by decomposing under reduced pressure and being dried by cooling, and then the powder containing anthocyanins can be obtained, and the extraction of the anthocyanins can be improved. content. n ·: 'The drying process described in Example 1 is directly subjected to a drying step, "to obtain an anthocyanin-containing powder. . . . , extraction method. The extracted lotus flower concentrate described in Example 1 is directly (4) (4), that is, _ powder containing alizarin. Qu..., extracting the human eve sugar method: the de-polysaccharide of the de-polysaccharide described in the example _ 彳 干燥 干燥 干燥 干燥 干燥 干燥 干燥 干燥 干燥 干燥 干燥 干燥 干燥 干燥 干燥 干燥 干燥 干燥 干燥 干燥 干燥Comparison table of total anthocyanin content obtained by the method:
僅利用水煮法所得到的總花青素含量為0 5 %,但經過水煮、 萃取，、去夕if法所彳于到的總花青素含量為G 67%，顯示本發明確 貝此提问萃取洛神化花青素含量。再以水煮法所得之總花青素含 里為基準胃比|x本發明萃取總花青素含量所提升的比例，其結 果見表2 : 總花青素含詈所楛井的比例 ----------- 化月百所 方法 提开的比例 經過水煮法 1 00 經過水煮與萃取法 1 16 經過水煮、萃取及去客觫.土 1.34 相較於—般水煮的方式，本發明經過水煮、萃取及去多醣法 1358261 .._可提养44-%-的-總44-— 青—素含量 歸納上述，本發明提高萃取洛神花花青素含量之方 致下述的功效及優點： <万法’可獲 一、經過水煮、萃取與去多The total anthocyanin content obtained by the boiling method alone is 0.5%, but the total anthocyanin content obtained by boiling, extraction, and the eve method is G 67%, indicating that the present invention is This question is extracted from the content of anthocyanin. The total anthocyanin content obtained by boiling method is used as the reference stomach ratio|x The ratio of the total anthocyanin content extracted by the present invention is increased. The results are shown in Table 2: The proportion of total anthocyanin containing strontium wells - ---------- The ratio of the method of the month is 100% boiled and extracted. 1 16 After boiled, extracted and decontaminated. Earth 1.34 compared to the same In the manner of boiled, the present invention is subjected to boiled, extracted and de-polysaccharide method 1348221 .._ can raise 44-%--total 44--cyan content. In summary, the present invention improves the content of anthocyanin extracted from Luoshen The following effects and advantages are achieved: <Wanfa' can be obtained, boiled, extracted and removed.
曰Λ 寺步驟’所得到的她芯主本A ….67%’相較於一般水煮 的…素合 34%β 弋其化月素的萃取量能提升 二、本發明所使用的溶劑t 較為安全。 因此於製造或使用時 惟以上所述者，僅為本發明之較佳實_而已，t不 限定本發明實施之範圍，及大凡依本 b此 内令所作之間單的等效變化與修飾， 曰 範圍内。 白應仍屬本發明專利涵蓋之 【圖式簡單說明】 第一圖係本發.明之流程圖。 【主要元件符號說明】 (a) 水煮步驟 (b) 萃取步驟 (c) 去多醣步驟 (d) 乾燥步驟The 芯 Temple step 'obtained her core main A ....67%' compared to the general boiled ... 34% β 弋 弋 化 化 的 的 的 的 的 的 的 的 的 的 的 的 的 的 的 的More secure. Therefore, the above is only the preferred embodiment of the present invention, and t is not limited to the scope of the present invention, and the equivalent variation and modification between the two according to the present invention. , within the scope of 曰. Bai Ying is still covered by the patent of the present invention. [Simplified description of the drawings] The first figure is a flow chart of the present invention. [Explanation of main component symbols] (a) boiling step (b) extraction step (c) de-polysaccharide step (d) drying step