CN105732830A - Extraction process for water-soluble polysaccharide of vine tea - Google Patents
Extraction process for water-soluble polysaccharide of vine tea Download PDFInfo
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- CN105732830A CN105732830A CN201410738873.2A CN201410738873A CN105732830A CN 105732830 A CN105732830 A CN 105732830A CN 201410738873 A CN201410738873 A CN 201410738873A CN 105732830 A CN105732830 A CN 105732830A
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- water
- ampelopsis grossedentata
- ethanol
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- distilled water
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Abstract
The invention relates to an extraction process for water-soluble polysaccharide of vine tea. The extraction process comprises the following steps: subjecting a finished vine tea product to crushing into coarse powder; then carrying out reflux with petroleum ether for degreasing and decoloring; carrying out hot dipping in 95% distilled water; then carrying out extraction with ethanol for 2 h; after centrifugation, pouring out supernatant; washing a precipitate with ethanol for 2 h and volatilizing ethanol; and dissolving the precipitate with distilled water so as to obtain crude polysaccharide extract.
Description
Technical field
The present invention relates to the extraction process of a kind of Water-soluble Polysaccharides from Ampelopsis grossedentata.
Background technology
Ampelopsis grossedentata, also known as mountain Folium hydrangeae strigosae, formal name used at school Ampelopsis grossedentata, its slightly sweet flavor, frightened, the effect such as there is heat-clearing and toxic substances removing, anti-inflammation wind-damp dispelling, blood pressure lowering, protect the liver.China its young stem and leaf of normal collection among the people, processed kneading, dry, make health tea use, so far existing centuries history.Ampelopsis grossedentata mainly contains flavones ingredient and carbohydrate, protein, the mineral matter and other components such as dihydromyricetin, ampelopsin.In recent years, the research report about the isolation identification of the particularly dihydromyricetin of flavones ingredient in Ampelopsis grossedentata and function thereof is more, and there is not been reported to the research of Ampelopsis grossedentata polysaccharide.
Summary of the invention
It is an object of the invention to provide the extraction process of a kind of Water-soluble Polysaccharides from Ampelopsis grossedentata.
The object of the present invention is achieved like this:
A, take west place in Hubei produce Ampelopsis grossedentata finished product, size-reduced and cross 20 mesh sieves obtain Ampelopsis grossedentata coarse powder, standby；
B, weigh appropriate Ampelopsis grossedentata coarse powder, reflux degreasing decoloring with petroleum ether, then add distilled water by certain ratio of water to material, water-bath hot dipping in uniform temperature carries certain time, a little after cooling, filters, filtrate is settled to 250ml, therefrom draws 10ml, after adding the ethanol mixing of 3 times of volumes, stand 2h, centrifugal (5000r/min, 2min), pour out supernatant, precipitate 2 times with ethanol in proper amount washing, volatilize ethanol, with distilled water dissolution precipitation and be settled to 100ml, it is liquid of extracting polysaccharide.
The positive effect of the present invention is in that:
Temperature, time and ratio of water to material are to affect the principal element that Water-soluble Polysaccharides from Ampelopsis grossedentata extracts.Center combination test and response analysis determine that Water-soluble Polysaccharides from Ampelopsis grossedentata preferably traditional extraction condition is temperature 95 DEG C, 25:1 ratio of water to material, extracts 4h, and once extracting polysaccharide yield was about 4.20% (representing with polyoses content).Extracting once, twice extraction ratio is up to 97% again.With 95% industrial alcohol precipitate polysaccharides of 3 times of volumes of extracting solution, standing 2h, crude polysaccharides yield higher (representing with crude product weight, extract up to about 7.4% for 2 times), work efficiency is fast.
Detailed description of the invention
Primarily determine that feasible Water-soluble Polysaccharides from Ampelopsis grossedentata with the integrated extraction technique route of total flavones is: Ampelopsis grossedentata coarse powder → petroleum ether degreasing decolouring → hot bath lixiviate → filtrate concentration with stand → separate total flavones → precipitate polysaccharides → separate crude polysaccharides.Under this skill condition, total flavones coarse powder and the crude polysaccharides yield of extracting twice respectively reached for 31% and more than 6.6% (representing with crude product weight).This process route cost is low, work efficiency fast, is suitable for large-scale production.
Claims (1)
1. an extraction process for Water-soluble Polysaccharides from Ampelopsis grossedentata, its concrete preparation method is as follows: a, takes west place in Hubei and produces Ampelopsis grossedentata finished product, size-reduced and cross 20 mesh sieves and Ampelopsis grossedentata coarse powder, standby；B, weigh appropriate Ampelopsis grossedentata coarse powder, reflux degreasing decoloring with petroleum ether, then add distilled water by certain ratio of water to material, water-bath hot dipping in uniform temperature carries certain time, a little after cooling, filters, filtrate is settled to 250ml, therefrom draws 10ml, after adding the ethanol mixing of 3 times of volumes, stand 2h, centrifugal (5000r/min, 2min), pour out supernatant, precipitate 2 times with ethanol in proper amount washing, volatilize ethanol, with distilled water dissolution precipitation and be settled to 100ml, it is liquid of extracting polysaccharide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410738873.2A CN105732830A (en) | 2014-12-08 | 2014-12-08 | Extraction process for water-soluble polysaccharide of vine tea |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410738873.2A CN105732830A (en) | 2014-12-08 | 2014-12-08 | Extraction process for water-soluble polysaccharide of vine tea |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105732830A true CN105732830A (en) | 2016-07-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410738873.2A Pending CN105732830A (en) | 2014-12-08 | 2014-12-08 | Extraction process for water-soluble polysaccharide of vine tea |
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| CN (1) | CN105732830A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106589150A (en) * | 2016-12-08 | 2017-04-26 | 西安交通大学 | Abelia engleriana polysaccharides and extraction method thereof |
| CN113854552A (en) * | 2021-09-23 | 2021-12-31 | 绿之韵生物工程集团有限公司 | Preparation method of polygonatum sibiricum enzyme |
| CN116217745A (en) * | 2022-12-23 | 2023-06-06 | 大连民族大学 | Vine tea polysaccharide, preparation method and application |
-
2014
- 2014-12-08 CN CN201410738873.2A patent/CN105732830A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106589150A (en) * | 2016-12-08 | 2017-04-26 | 西安交通大学 | Abelia engleriana polysaccharides and extraction method thereof |
| CN106589150B (en) * | 2016-12-08 | 2019-07-23 | 西安交通大学 | A kind of brachyplast Abelia biflora leaf polyose and its extracting method |
| CN113854552A (en) * | 2021-09-23 | 2021-12-31 | 绿之韵生物工程集团有限公司 | Preparation method of polygonatum sibiricum enzyme |
| CN116217745A (en) * | 2022-12-23 | 2023-06-06 | 大连民族大学 | Vine tea polysaccharide, preparation method and application |
| CN116217745B (en) * | 2022-12-23 | 2024-04-26 | 大连民族大学 | Vine tea polysaccharide, preparation method and application |
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Legal Events
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| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160706 |