CN103933209A - Method for extracting steroid saponin from Chinese lantern-plant stems and persistent calyx - Google Patents
Method for extracting steroid saponin from Chinese lantern-plant stems and persistent calyx Download PDFInfo
- Publication number
- CN103933209A CN103933209A CN201410131662.2A CN201410131662A CN103933209A CN 103933209 A CN103933209 A CN 103933209A CN 201410131662 A CN201410131662 A CN 201410131662A CN 103933209 A CN103933209 A CN 103933209A
- Authority
- CN
- China
- Prior art keywords
- saponin
- extracting
- drugs
- calyx
- plant stems
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Preparation Of Fruits And Vegetables (AREA)
- Steroid Compounds (AREA)
Abstract
The invention relates to a method for extracting steroid saponin from Chinese lantern-plant stems and persistent calyx, relating to a drug extracting method, which comprises the following steps of crushing dried Chinese lantern-plant stems and persistent calyx, then extracting for 1-5 times and 5-15 min with the microwave radiation power of 380-680 W and 60-90% ethanol, wherein the material-to-liquid ratio is 1:10 to 1:40; after extracting, filtering, extracting, mixing extraction solutions to prepare an extract; after the extract is dissolved, measuring through a vanillic aldehyde-perchloric acid colorimetric method based on dioscin as the standard substance to calculate the content of saponin in the extract. By means of the microwave extraction method, the extraction time can be obviously shortened; the yield of steroid saponin is increased; the medicinal and edible dual-purpose resources of steroid saponin in the Chinese lantern-plant stems can be sufficiently utilized; in addition, after being separated and purified, steroid saponin in Chinese lantern-plant stems and persistent calyx extracted through the method disclosed by the invention can be used for preparing the drugs, such as antibacterial drugs, expectorant drugs, antiplatelet drugs, lipid-lowering drugs and anti-tumour drugs, or used as the raw material of functional foods and health-care foods.
Description
Technical field
The present invention relates to one and relate to extract drugs method, particularly relate to a kind of method of extracting steroidal saponin from Calyx Seu Fructus Physalis stem and Constellation calyx.
Background technology
Calyx Seu Fructus Physalis (
physalis alkekengi), another name Calyx seu fructus physalis, hang up a Chinese lantern, Calyx Seu Fructus Physalis etc., be Solanaceae monkey flower, fruit is that spheroidal berry is perennial root draft, its fruit is edible, is China's time-honored medicine-food two-purpose health fruit.In its successive dynasties book on Chinese herbal medicine, also record.Main product in the Three Eastern Provinces, the ground such as Hebei, Xinjiang, Shandong.The composition of Calyx Seu Fructus Physalis mainly contains steroid, sterols, alkaloids, amino acids, volatile oil, polysaccharide, inorganic elements etc.Pharmacological research shows that Calyx Seu Fructus Physalis has antiinflammatory, malaria, the well pharmacological action such as anticancer, antibacterial.According to the basic structure of sapogenin, saponin is divided into steroidal saponin and triterpene saponin.The saponin containing in Calyx Seu Fructus Physalis is mainly steroidal saponin, and research shows that dioscin is different spirostane alcohol type steroidal saponin, its have eliminate the phlegm, the effect such as antiplatelet aggregation, blood fat reducing, antitumor.According to another bibliographical information, the steroidal saponin in franchet groundcherry calyx or fruit and diabetes pill be similar all has blood sugar reducing function.And with Calyx Seu Fructus Physalis in the triterpene saponin of steroidal saponin similar; as ginsenoside Rg1 can protect dopamine neuron; can reduce lipid content treatment of vascular and can also prevent cerebral atherosclerosis, can suppress the effects such as the expression of blood vessel endothelium matrix metalloproteinase.
The principle of microwave extraction is mainly that the process that microwave radiation conducts heat is that frequency electromagnetic waves passes in the inner cell of medium arrival extract, thereby the electromagnetic field of microwave can accelerate the molecular diffusion rate of extract components.Can determine that by microwave principle the inventive method can improve extraction efficiency, and save time.The extraction of Calyx Seu Fructus Physalis steroidal saponin in the past mainly adopts water-bath Effect of Alcohol Extracting Procedureto Total, and when the method operating cost, loaded down with trivial details, product yield is low, and energy consumption is large.
Summary of the invention
The object of the present invention is to provide a kind of method of extracting steroidal saponin from Calyx Seu Fructus Physalis stem and Constellation calyx, the method is by after dry Calyx Seu Fructus Physalis stem and Constellation calyx pulverizing, extract feed liquid, make extractum, medicinal and the edible resource of exploitation Calyx Seu Fructus Physalis stem, meanwhile, microwave extraction technique can obviously reduce extraction time, improve the yield of saponin.The Calyx Seu Fructus Physalis stem obtaining through the inventive method and the steroidal saponin of Constellation calyx through separation and purification can be used as preparation antibacterial, eliminate the phlegm, the medicine of antiplatelet aggregation, blood fat reducing, antitumor etc. or use as the raw material of functional food, health product.
The object of the invention is to be achieved through the following technical solutions:
From Calyx Seu Fructus Physalis stem and Constellation calyx, extract a method for steroidal saponin, described method comprises following process:
A. sample treatment:
Calyx Seu Fructus Physalis stem is dried in 100～105 DEG C of baking ovens, is ground into Powderedly with pulverizer, crosses 40～80 mesh sieve postpones and in dry valve bag, seals up for safekeeping stand-by;
B. microwave method is extracted:
Accurate 1～5g stem or 0.1～0.5g Constellation calyx powder, measure concentration of alcohol and be 60%～90% in 250ml round-bottomed flask, according to set experimental program, extracts 1～5 time, extracts 5min～15min, solid-liquid ratio 1:10～1:40; Under microwave irradiation power 380～680W condition, carry out microwave extraction; After extraction, sucking filtration, concentrated by rotary evaporation, shifts out and makes extractum, stand-by;
C. the foundation of standard curve:
Take dioscin standard substance in volumetric flask, be made into titer with absolute methanol standardize solution, get after titer adds the colour developing of vanillin-perchloric acid and carry out length scanning, record maximum wavelength; Pipette respectively the titer of quantitative gradient dilution in tool plug test tube, after water-bath volatilizes, add respectively 0.1～0.5ml, 2～5% vanillin-glacial acetic acid liquid, 0.5～1.0ml perchloric acid, 65～70 DEG C of water-baths, heating 10～15min, frozen water is cooling; Add 4.0～6.0ml glacial acetic acid to leave standstill 10～30min, measure absorbance at maximum wavelength place, taking absorbance (A) as vertical coordinate Y, the concentration C of dioscin is that abscissa X does standard curve, and sets up regression equation;
D. the mensuration of the content of steroidal saponin:
Adopt vanillin-perchloric acid to measure the content of saponin: the extract revolving after steaming is settled in volumetric flask; Pipette quantitative above-mentioned solution and be placed in tool plug test tube, water-bath volatilizes solvent; Then add 0.1～0.5ml, vanillin-glacial acetic acid solution of 2～5% and 0.5～1.0ml perchloric acid, close plug test tube shakes up, and is placed in 65～70 DEG C of water-bath vibration heating 10 ~ 15min; After taking-up, use immediately frozen water cooling; Add 4.0～6.0ml glacial acetic acid to mix, leave standstill 10～30min.Measure absorbance in maximum absorption wave strong point; According to the regression equation calculation saponin yield of the absorbance substitution standard curve measuring;
Above-mentioned percentage ratio is percent by volume.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described.
Embodiment 1:
(1) Calyx Seu Fructus Physalis stem is dried in 100～105 DEG C of baking ovens, is ground into Powderedly with pulverizer, crosses 40～80 mesh sieve postpones and in dry valve bag, seals up for safekeeping stand-by;
(2) the foundation of standard curve: take 5～10mg dioscin and be made into absolute methanol standardize solution in 50ml volumetric flask the titer of 0.05～0.2mg/ml, get after titer adds the colour developing of vanillin-perchloric acid and carry out length scanning, maximum wavelength is 500～560nm.Pipette respectively 300,400,500,1100,1300,1500ul in tool plug test tube, after water-bath volatilizes, add respectively 0.1～0.5ml(2～5%) vanillin-glacial acetic acid liquid, 0.5～1.0ml perchloric acid, 65～70 DEG C of water-baths, heating 10～15min, frozen water is cooling; Add 4.0～6.0ml glacial acetic acid to leave standstill 10～30min, measure absorbance at 560nm place.Taking absorbance (A) as vertical coordinate Y, the concentration C of dioscin is that abscissa X does standard curve, and sets up regression equation;
(3) accurately take three parts of 1～5g Calyx Seu Fructus Physalis stem powder, with 60～90% alcoholic solution, extraction 5～15min, extraction 1～5 time, solid-liquid ratio 1:10～1:40, under radiant power 380～680W condition, extract respectively;
(4) extract complete, sucking filtration, concentrated by rotary evaporation, shifts out, and makes extractum, stand-by;
(5) measure contents of steroid saponin with vanillin-perchloric acid, the extract revolving after steaming is settled in 100ml volumetric flask; Pipette 0.1ml and be placed in 10ml tool plug test tube, 60 DEG C of water-baths volatilize solvent; Then add 0.1～0.5ml(2～5%) vanillin-glacial acetic acid liquid, 0.5～1.0ml perchloric acid, 65～70 DEG C of water-baths, heating 10～15min, frozen water is cooling; Add 4.0～6.0ml glacial acetic acid to leave standstill 10～30min, measure absorbance in maximum absorption wave strong point.According to the regression equation of the absorbance substitution standard curve measuring.Be greater than 5.0% by the steroidal saponin yield that calculates stem.
Embodiment 2:
(1) franchet groundcherry calyx or fruit is dried in 100～105 DEG C of baking ovens, is ground into Powderedly with pulverizer, crosses 40～80 mesh sieve postpones and in dry valve bag, seals up for safekeeping stand-by;
(2) the foundation of standard curve: (2) identical with embodiment 1.
(3) accurately take three parts of 0.1～0.5g franchet groundcherry calyx or fruit powder, with 60～90% alcoholic solution, extraction 5～15min, extraction 1～5 time, solid-liquid ratio 1:10～1:40, under radiant power 380～680W condition, extract respectively;
(4) extract complete, sucking filtration, concentrated by rotary evaporation, shifts out, and makes extractum, stand-by;
(5) measure contents of steroid saponin with vanillin-perchloric acid, the extract revolving after steaming is settled in 100ml volumetric flask; Pipette 0.1ml and be placed in 10ml tool plug test tube, 60 DEG C of water-baths volatilize solvent; Then add 0.1～0.5ml(2～5%) vanillin-glacial acetic acid liquid, 0.5～1.0ml perchloric acid, 65～70 DEG C of water-baths, heating 10～15min, frozen water is cooling; Add 4.0～6.0ml glacial acetic acid to leave standstill 10～30min, measure absorbance in maximum absorption wave strong point.According to the regression equation of the absorbance substitution standard curve measuring.Be greater than 8.0% by calculating steroidal saponin yield in Constellation calyx.
Claims (1)
1. a method of extracting steroidal saponin from Calyx Seu Fructus Physalis stem and Constellation calyx, is characterized in that, described method comprises following process:
A. sample treatment:
Calyx Seu Fructus Physalis stem is dried in 100～105 DEG C of baking ovens, is ground into Powderedly with pulverizer, crosses 40～80 mesh sieve postpones and in dry valve bag, seals up for safekeeping stand-by;
B. microwave method is extracted:
Accurate 1 ~ 5g stem or 0.1～0.5g Constellation calyx powder, measure concentration of alcohol and be 60%～90% in 250ml round-bottomed flask, according to set experimental program, extracts 1～5 time, extracts 5min～15min, solid-liquid ratio 1:10～1:40; Under microwave irradiation power 380～680W condition, carry out microwave extraction; After extraction, sucking filtration, concentrated by rotary evaporation, shifts out and makes extractum, stand-by;
C. the foundation of standard curve:
Take dioscin standard substance in volumetric flask, be made into titer with absolute methanol standardize solution, get after titer adds the colour developing of vanillin-perchloric acid and carry out length scanning, record maximum wavelength; Pipette respectively the titer of quantitative gradient dilution in tool plug test tube, after water-bath volatilizes, add respectively 0.1～0.5ml, 2～5% vanillin-glacial acetic acid liquid, 0.5～1.0ml perchloric acid, 65～70 DEG C of water-baths, heating 10～15min, frozen water is cooling; Add 4.0～6.0ml glacial acetic acid to leave standstill 10～30min, measure absorbance at maximum wavelength place, taking absorbance (A) as vertical coordinate Y, the concentration C of dioscin is that abscissa X does standard curve, and sets up regression equation;
D. the mensuration of the content of steroidal saponin:
Adopt vanillin-perchloric acid to measure the content of saponin: the extract revolving after steaming is settled in volumetric flask; Pipette quantitative above-mentioned solution and be placed in tool plug test tube, water-bath volatilizes solvent; Then add 0.1～0.5ml, vanillin-glacial acetic acid solution of 2～5% and 0.5～1.0ml perchloric acid, close plug test tube shakes up, and is placed in 65～70 DEG C of water-bath vibration heating 10 ~ 15min; After taking-up, use immediately frozen water cooling; Add 4.0～6.0ml glacial acetic acid to mix, leave standstill 10～30min; Measure absorbance in maximum absorption wave strong point; According to the regression equation calculation saponin yield of the absorbance substitution standard curve measuring; Above-mentioned percentage ratio is percent by volume.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410131662.2A CN103933209B (en) | 2014-04-03 | 2014-04-03 | A kind of method extracted in the stem from wintercherry fruit or calyx and detect steroid saponin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410131662.2A CN103933209B (en) | 2014-04-03 | 2014-04-03 | A kind of method extracted in the stem from wintercherry fruit or calyx and detect steroid saponin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103933209A true CN103933209A (en) | 2014-07-23 |
| CN103933209B CN103933209B (en) | 2018-03-13 |
Family
ID=51181266
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410131662.2A Active CN103933209B (en) | 2014-04-03 | 2014-04-03 | A kind of method extracted in the stem from wintercherry fruit or calyx and detect steroid saponin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103933209B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105816569A (en) * | 2015-01-08 | 2016-08-03 | 辽宁医联新药研究所 | Method for extracting calyx seu fructus physalis anti-pharyngitis effective part and its total steroid as well as refining method thereof |
| CN108148107A (en) * | 2017-12-25 | 2018-06-12 | 华远医药研究院有限公司 | The method for extraction and purification of Dioscin |
| CN113583470A (en) * | 2021-07-31 | 2021-11-02 | 彼悦(北京)科技有限公司 | Multifunctional composite plant dye and preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1742845A (en) * | 2005-09-28 | 2006-03-08 | 大连美罗中药厂有限公司 | Medicine for treating rheumatism and rheumatoid diseases and preparing method |
| CN1864717A (en) * | 2006-06-14 | 2006-11-22 | 哈尔滨医科大学 | Method for extracting Chinese lantern plant steride and pharmaceutical composition thereof |
| CN2864717Y (en) * | 2005-12-08 | 2007-01-31 | 陈广生 | Anti-tension type jig for optical fiber composite phase wire |
| CN101244153A (en) * | 2008-02-01 | 2008-08-20 | 张炯怡 | Application of alkekengi in preparing medicament for treating digestive system disease, product and preparation |
-
2014
- 2014-04-03 CN CN201410131662.2A patent/CN103933209B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1742845A (en) * | 2005-09-28 | 2006-03-08 | 大连美罗中药厂有限公司 | Medicine for treating rheumatism and rheumatoid diseases and preparing method |
| CN2864717Y (en) * | 2005-12-08 | 2007-01-31 | 陈广生 | Anti-tension type jig for optical fiber composite phase wire |
| CN1864717A (en) * | 2006-06-14 | 2006-11-22 | 哈尔滨医科大学 | Method for extracting Chinese lantern plant steride and pharmaceutical composition thereof |
| CN101244153A (en) * | 2008-02-01 | 2008-08-20 | 张炯怡 | Application of alkekengi in preparing medicament for treating digestive system disease, product and preparation |
Non-Patent Citations (4)
| Title |
|---|
| 刘雅丽，等: "锦灯笼宿萼皂苷降血糖作用研究", 《东北师大学报（自然科学版）》 * |
| 孟庆然，等。: "锦灯笼酸浆宿萼皂苷提取工艺的研究", 《山西农业大学学报》 * |
| 郭晓宇，等。: "中药材皂苷提取方法与工艺研究", 《内蒙古医科大学学报》 * |
| 郭祀远等，: "《溶液过程理论与电磁处理技术》", 31 July 2009 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105816569A (en) * | 2015-01-08 | 2016-08-03 | 辽宁医联新药研究所 | Method for extracting calyx seu fructus physalis anti-pharyngitis effective part and its total steroid as well as refining method thereof |
| CN108148107A (en) * | 2017-12-25 | 2018-06-12 | 华远医药研究院有限公司 | The method for extraction and purification of Dioscin |
| CN113583470A (en) * | 2021-07-31 | 2021-11-02 | 彼悦(北京)科技有限公司 | Multifunctional composite plant dye and preparation method and application thereof |
| CN113583470B (en) * | 2021-07-31 | 2023-05-23 | 彼悦(北京)科技有限公司 | Multifunctional composite vegetable dye and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103933209B (en) | 2018-03-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101942355A (en) | Comprehensive extraction method for extracting tea seed oil, tea saponin and tea seed polysaccharide from tea seeds or camellia seeds | |
| CN102133266B (en) | Preparation method for macroporous resin purifying extract of golden hemorrhoid fumigating-washing powder | |
| CN101336987A (en) | Preparation method of total flavone of Hovenia dulcisThunb | |
| CN103142669A (en) | Enzyme method for extracting ginsenoside and obtained ginsenoside extractive | |
| CN110051726A (en) | The preparation method and application of general flavone and total starches in a kind of Qingqian Willow leaf | |
| CN104262423A (en) | Method for extracting phlorhizin from litchi rind | |
| CN104592323B (en) | A kind of method of phloridzin in aqueous two-phase extraction pomace | |
| CN101524458B (en) | Extract of smilax glabra saponins and flavonoid and preparation method thereof | |
| CN105079050B (en) | A kind of method preparing the extract superfine powder rich in Semen Ginkgo polysaccharide | |
| CN103933209A (en) | Method for extracting steroid saponin from Chinese lantern-plant stems and persistent calyx | |
| CN103463160A (en) | Preparation method of high-content total flavones of chrysanthemum | |
| CN104940280A (en) | Method for extracting total flavones from radix puerariae employing enzyme preparation | |
| CN103705647A (en) | Process method for extracting general flavone of golden camellia leaves by CO2 supercritical method | |
| CN103467262B (en) | Method for preparing 9-oxonerolidol from camphor tree plants | |
| CN104257701A (en) | Method for preparing lucid ganoderma triterpenoids | |
| CN102940724A (en) | Preparation method of traditional Chinese medicine compound capsule for diminishing inflammation | |
| CN104083505B (en) | A kind of extracting method of acid Fructus Litchi ethanol position active component | |
| CN1943647B (en) | The method for preparing triterpenic acid extract from the loquat leaves | |
| CN104721240A (en) | Process for extracting and separating moringa oleifera flavones and saponin from moringa oleifera leaves | |
| CN103319563B (en) | Extraction and purification method of soyasaponins | |
| CN101972283B (en) | Preparation method of common wedgelet fern herb total flavone | |
| Pradhan et al. | Recent advancements in the extraction of phytoconstituents from herbal sources | |
| CN103385950A (en) | A preparation method for total rose laevigata michx flavones | |
| CN103099827A (en) | Extraction preparation method for flavones from grass leaves with blood enriching | |
| CN104382966B (en) | A kind of extracting method for smoking the general flavone of falling ox |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Gao Pinyi Inventor after: Liu Xuegui Inventor after: Gao Yixing Inventor after: Wang Le Inventor after: Zhang Zhenxue Inventor before: He Haitao Inventor before: Sun Yanyan Inventor before: Wan Yanping Inventor before: Jiang Fuyu Inventor before: Liu Xuegui Inventor before: Gao Pinyi |
|
| COR | Change of bibliographic data | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |