CN105816569A - Method for extracting calyx seu fructus physalis anti-pharyngitis effective part and its total steroid as well as refining method thereof - Google Patents
Method for extracting calyx seu fructus physalis anti-pharyngitis effective part and its total steroid as well as refining method thereof Download PDFInfo
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Abstract
The invention discloses a method for extracting and refining total steroid of a calyx seu fructus physalis anti-pharyngitis effective part as well as its total steroid prepared by the preparation method thereof. The total steroid is obtained through extraction in calyx seu fructus physalis, macroporous resin separation and refining. Through purification in the method, the total steroid content in an extract product can reach more than 70%, and recovery rate of the total steroid is more than 85%, and the method can be a refining technology for the calyx seu fructus physalis total steroid. The method has the advantages of simple operation, simple equipment, less solvent pollution, and low production cost. The method is stable and feasible, and is suitable for industrial production, and can be the extraction and refining technology of the total steroid of calyx seu fructus physalis.
Description
Technical field
The invention belongs to field of medicaments, relate to one and extract from Calyx Seu Fructus Physalis medical material and refine total steroidal technique.
Background technology
Show according to the World Health Organization's investigation, the whole world have 80% Nasopharyngeal Carcinoma Patients occur in China, the rhinitis of China, pharyngitis patient have about 300,000,000, every year with 3% speed increase, the annual death toll caused because of nasopharynx canceration is more than 200,000 people, and the age tends to become younger.The features such as rhinitis, pharyngitis are high with sickness rate, complication is many, refractory is healed cause the great attention of medical circle.Root according to the literature, franchet groundcherry calyx or fruit have sore-throat relieving reduce phlegm, the curative effect of heat-clearing and toxic substances removing.
Pharyngitis diseased region is special, and the cause of disease is complicated, and symptom is obstinate, easy recurrent exerbation.At present, the treatment of pharyngitis uses antibiotics to treat the most mostly, and conventional has gentamycin, penicillin, dexamethasone and Roxithromycin, amoxicillin, Dobell's solution, nitrofural collutory etc..Increasingly severe to antibiotics resistance due to microorganism in recent years, make the clinical practice of antibiotics be restricted, therapeutic effect is the most highly desirable.Chinese medicine pharyngitis, has that toxic and side effects is little, action temperature and a lasting feature, becomes medical science and the focus of attention of pharmaceutical researchers in recent years.
Calyx Seu Fructus Physalis is Solanaceae Physalis herbaceos perennial, the dry Constellation calyx that Calyx Seu Fructus Physalis medical material is PhysalisalkekengiLvar.francheti (Mast.) Makino that " Chinese Pharmacopoeia " version in 2010 is recorded or the Constellation calyx of band fruit.Calyx Seu Fructus Physalis plant is distributed widely in national each provinces and regions, and fruit, root and herb all can be used as medicine.Calyx Seu Fructus Physalis medical material begins to be loaded in Shennong's Herbal, name Chinese lantern plant, is classified as middle product.Thereafter history tree records more.Calyx Seu Fructus Physalis dampness removing heat extraction described in bright Li Shizhen (1518-1593 A.D.) Compendium of Material Medica, lung heat clearing controls and coughs, and dampness removing reduces phlegm, and controls cellulitis [1].One record of " Chinese Pharmacopoeia " version in 2010, its sour in the mouth, hardship, cold in nature, there is heat clearing away, the effect such as removing toxic substances, sore-throat relieving reduce phlegm, diuresis, for pharyngalgia, hoarseness, phlegm-heat cough, dysuria,
External treatment pemphigus, eczema.
By consulting literatures, find the main component that total steroidal is antiinflammatory in Calyx Seu Fructus Physalis.At present, extract total steroidal in separation Calyx Seu Fructus Physalis and lack related data and report.
Summary of the invention
First purpose of the present invention is to provide a kind of method that technique is simple, low cost, efficiency are high, be suitable for the total steroidal of Hydrolysis kinetics Calyx Seu Fructus Physalis of industrialized production.By reflux, extract, from Calyx Seu Fructus Physalis, separate with AB-8 macroporous resin, refined, obtain the total steroidal of Calyx Seu Fructus Physalis.
Extraction of the present invention and the method for the refined refined total steroidal of Calyx Seu Fructus Physalis, is characterized in that, extraction process is the ethanol extraction 2 times of Calyx Seu Fructus Physalis 30 times amount 70%, each 2h.Process for refining is to select AB-8 type macroporous resin, takes Calyx Seu Fructus Physalis extract solution 8.5mg/ml ~ 16.5mg/ml, adsorbs 1h, ethanol with 50% carries out eluting, and flow velocity is 2BV/h, collects the eluent between 0.3 ~ 0.8BV, it is evaporated to do, obtains Calyx Seu Fructus Physalis total steroidal extract.
Second object of the present invention is to provide a kind of purity total steroidal of higher Calyx Seu Fructus Physalis, can be widely used in enterprises and produce.
It is an advantage of the invention that the purification through this technique, in gained Calyx Seu Fructus Physalis total steroidal extract, total steroidal content all reaches more than 70%, and the response rate of total steroidal reaches more than 85%, provides the raw material of high-quality for producing of Calyx Seu Fructus Physalis medicine.
Detailed description of the invention
Following example provide the detailed description of the invention of foregoing invention, but they are not limited to the gamut of the present invention.
Embodiment 1
A kind of Calyx Seu Fructus Physalis anti-pharyngitis total steroidal of effective site extracts and refined method, and including following steps, (1) abstraction process (2) separates and refining step, i.e. prepares finished product, wherein；
(1) abstraction process: weigh Calyx Seu Fructus Physalis 5g and put in container, use ethanol reflux extraction, i.e. add 50% ethanol taking brocade lamp weight 20 times amount, heating and refluxing extraction 1 time, extraction time is 1.5 hours every time, sucking filtration while hot, merging filtrate, recovery filtrate is to without alcohol, and water liquid is concentrated into paste and obtains extractum.
(2) separation and refining step: by the extractum solution obtained by step 1 abstraction process, preparation 8.5mg/ml concentration, with AB-8 type macroporous resin chromatography, absorption 3h, ethanol with 50% carries out eluting, and flow velocity is 1BV/h, collects the eluent between 0.1 ~ 0.9BV, it is evaporated to do, obtains Calyx Seu Fructus Physalis total steroidal extract.
Embodiment 2
A kind of Calyx Seu Fructus Physalis anti-pharyngitis total steroidal of effective site extracts and refined method, and including following steps, (1) abstraction process (2) separates and refining step, i.e. prepares finished product, wherein；
(1) abstraction process: weigh Calyx Seu Fructus Physalis 5g and put in container, use ethanol reflux extraction, i.e. add 95% ethanol taking brocade lamp weight 40 times amount, heating and refluxing extraction 3 times, extraction time is 2.5 hours every time, sucking filtration while hot, merging filtrate, recovery filtrate is to without alcohol, and water liquid is concentrated into paste and obtains extractum.
(2) separation and refining step: by the extractum solution obtained by step 1 abstraction process, preparation 8.5mg/ml concentration, with AB-8 type macroporous resin chromatography, absorption 2h, ethanol with 50% carries out eluting, and flow velocity is 2BV/h, collects the eluent between 0.1 ~ 0.9BV, it is evaporated to do, obtains Calyx Seu Fructus Physalis total steroidal extract.
Embodiment 3
A kind of Calyx Seu Fructus Physalis anti-pharyngitis total steroidal of effective site extracts and refined method, and including following steps, (1) abstraction process (2) separates and refining step, i.e. prepares finished product, wherein；
(1) abstraction process: weigh Calyx Seu Fructus Physalis 5g and put in container, use ethanol reflux extraction, i.e. add 95% ethanol taking brocade lamp weight 30 times amount, heating and refluxing extraction 2 times, extraction time is 2 hours every time, sucking filtration while hot, merging filtrate, recovery filtrate is to without alcohol, and water liquid is concentrated into paste and obtains extractum.
(2) separation and refining step: by the extractum solution obtained by step 1 abstraction process, preparation 10.0mg/ml concentration, with AB-8 type macroporous resin chromatography, absorption 1h, ethanol with 50% carries out eluting, and flow velocity is 2BV/h, collects the eluent between 0.2 ~ 0.8BV, it is evaporated to do, obtains Calyx Seu Fructus Physalis total steroidal extract.
Embodiment 4
A kind of Calyx Seu Fructus Physalis anti-pharyngitis total steroidal of effective site extracts and refined method, and including following steps, (1) abstraction process (2) separates and refining step, i.e. prepares finished product, wherein；
(1) abstraction process: weigh Calyx Seu Fructus Physalis 5g and put in container, use ethanol reflux extraction, i.e. add 70% ethanol taking brocade lamp weight 30 times amount, heating and refluxing extraction 2 times, extraction time is 2.5 hours every time, sucking filtration while hot, merging filtrate, recovery filtrate is to without alcohol, and water liquid is concentrated into paste and obtains extractum.
(2) separation and refining step: by the extractum solution obtained by step 1 abstraction process, preparation 15.0mg/ml concentration, with AB-8 type macroporous resin chromatography, absorption 2h, ethanol with 50% carries out eluting, and flow velocity is 2BV/h, collects the eluent between 0.2 ~ 0.8BV, it is evaporated to do, obtains Calyx Seu Fructus Physalis total steroidal extract.
Embodiment 5
A kind of Calyx Seu Fructus Physalis anti-pharyngitis total steroidal of effective site extracts and refined method, and including following steps, (1) abstraction process (2) separates and refining step, i.e. prepares finished product, wherein；
(1) abstraction process: weigh Calyx Seu Fructus Physalis 5g and put in container, use ethanol reflux extraction, i.e. add 70% ethanol taking brocade lamp weight 30 times amount, heating and refluxing extraction 2 times, extraction time is 2 hours every time, sucking filtration while hot, merging filtrate, recovery filtrate is to without alcohol, and water liquid is concentrated into paste and obtains extractum.
(2) separation and refining step: by the extractum solution obtained by step 1 abstraction process, preparation 15.0mg/ml concentration, with AB-8 type macroporous resin chromatography, absorption 1h, ethanol with 50% carries out eluting, and flow velocity is 2BV/h, collects the eluent between 0.3 ~ 0.8BV, it is evaporated to do, obtains Calyx Seu Fructus Physalis total steroidal extract.
In Vitro Bacteriostasis
Macroporous resin water elution position group, 50% alcohol elution group, 95% alcohol elution group concentration being joined for 0.2532g/mL, 0.0264g/mL, 0.0128g/mL respectively, positive drug ceftriaxone sodium 0.2548mg/mL concentration is standby.Sterile working, takes the teat glass 10 of 5mL, aseptic broth bouillon, and the 1st pipe adds broth bouillon 1.5mL, and 2 ~ 10 pipes are separately added into 1mL；0.5mL medicinal liquid is added in the 1st pipe, mixing is taken out 1mL and is added in the 2nd pipe, from the 2nd pipe, take out 1mL add in the 3rd pipe, medicinal liquid two-fold dilution becomes the 1: 4,1: 8,1: 16,1: 32,1: 64,1: 128,1: 258,1: 512,1: 1024, the 9th pipe sucking-off 1mL discard successively, and the 10th pipe is not added with medicinal liquid and only adds 1mL broth bouillon, each pipe all adds, except the 9th pipe, the bacteria suspension that 50 μ L prepare, each test tube is jumped a queue, and cultivates 18h, is respectively repeated 3 times for 37 DEG C.Result judgement: with perusal, the 9th pipe does not add bacterium solution, all clarifies, and the 10th pipe does not add medicinal liquid, and all muddy, the high extract extension rate that the 1st ~ 8 pipe changes there is not muddiness is minimal inhibitory concentration MIC.Select made MIC each group, the Tube propagation liquid inoculating loop of all clear asepsis growths in group is dipped and is inoculated on a small quantity on Agar Plating, put in 37 DEG C of constant incubators, cultivate 18h, be respectively repeated 3 times.Result judges: do not have the lowest concentration of drug of bacterial growth to be minimal bactericidal concentration MBC.
Table 1 minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC) measurement result
| Water elution position mg/mL | 50% alcohol elution mg/mL | 95% alcohol elution mg/mL | Ceftriaxone sodium μ g/mL | |
| Staphylococcus aureus MIC | 15.83 | 1.65 | — | 8.00 |
| Staphylococcus aureus MBC | 31.65 | 6.60 | — | 16.00 |
| Staphylococcus epidermidis MIC | 15.83 | 1.65 | — | 8.00 |
| Staphylococcus epidermidis MBC | 15.83 | 3.30 | — | 16.00 |
| Staphylococcus saprophyticus MIC | 15.83 | 3.30 | — | 8.00 |
| Staphylococcus saprophyticus MBC | 15.83 | 6.60 | — | 16.00 |
| Escherichia coli MIC | 15.83 | 3.30 | — | 4.00 |
| Escherichia coli MBC | 15.83 | 6.60 | — | 8.00 |
| Bacillus paratyphosus B MIC | 15.83 | 1.65 | — | 0.06 |
| Bacillus paratyphosus B MBC | 31.65 | 3.30 | — | 8.00 |
| Shigella flexneri MIC | 15.83 | 1.65 | — | 0.06 |
| Shigella flexneri MBC | 31.65 | 6.60 | — | 8.00 |
| Beta hemolytic streptococcus MIC | 15.83 | 3.30 | — | 8.00 |
| Bacillus pyocyaneus MIC | 31.65 | 3.30 | — | 0.06 |
| Bacillus pyocyaneus MBC | 63.30 | 13.20 | — | 8.00 |
" " represents do not have antibacterial and bactericidal action
In extract obtained each eluting position, the antibacterial and bacteriocidal concentration of Calyx Seu Fructus Physalis 50% alcohol elution is minimum, effective.
Internal antiinflammatory action
Take mice 60, male, body weight 20 ± 2g, it is randomly divided into 6 groups by body weight, often group 10.It is respectively model
Group, positive drug control group, Calyx Seu Fructus Physalis 50% alcohol elution high dose group, two dosage groups of low dosage；Calyx Seu Fructus Physalis water elution position high dose, two dosage groups of low dosage.The equal gastric infusion of each group, model group is to consubstantiality hydrops, and continuous 7 days, proinflammatory agent was dimethylbenzene.
Before experiment, water 18h is can't help in mice fasting, 30min after last administration, auris dextra tow sides mice are uniformly coated with dimethylbenzene 0.1mL, after 2h, de-cervical vertebra is put to death, two ears are cut along auricle baseline, weighing in the punching of left and right ear same area with 6mm card punch, record swelling, auris dextra weight subtracts left ear weight and is swelling.
The impact of table 2 Calyx Seu Fructus Physalis effective site xylol induced mice ear swelling (X± S, n=10)
| Group | Dosage (mg/kg) | Ear edema degree (mg) | Inhibitory rate of intumesce (%) |
| Model group | - | 9.45±2.02 | - |
| Aspirin group | 200.0 | 5.45±3.07** | 42.30 |
| 50% position high dose group | 330.0 | 5.11±3.70** | 45.90 |
| 50% position low dose group | 82.5 | 6.36±3.81* | 32.70 |
| Water position high dose group | 816.0 | 7.49±3.36 | 20.70 |
| Water position low dose group | 204.0 | 7.99±3.01 | 15.45 |
Compared with model group, * * P < 0.01, * P < 0.05
Calyx Seu Fructus Physalis 50% alcohol elution has significantly suppression mice ear effect caused by dimethylbenzene, and has pole significant difference during high dose.The inhibitory action of the mice ear caused by Calyx Seu Fructus Physalis 50% alcohol elution high dose group xylol is better than positive controls.
Pharyngitis is mainly infected by virus, antibacterial and chemical factors stimulation causes, and is divided into acute pharyngitis and chronic pharyngitis two kinds.Acute pharyngitis is the acute inflammation of pharyngeal mucous membrane, submucous tissue.Chronic pharyngitis is under pharyngeal mucosa, mucosa and adenoid diffusivity inflammation, is often a part for upper respiratory tract chronic inflammatory disease.The material in Calyx Seu Fructus Physalis with bacteriostasis and antiinflammatory action is concentrated mainly on macroporous resin 50% alcohol elution, therefore is defined as the effective site of Calyx Seu Fructus Physalis treatment pharyngitis.
Claims (8)
1. the extraction of the total steroidal of Calyx Seu Fructus Physalis and a process for refining, be characterised by, it includes abstraction process and refining step:
(1) abstraction process: weigh dry Calyx Seu Fructus Physalis, uses extracting method to obtain extracting solution, and after sucking filtration, concentrated filtrate is to paste, obtains extractum；
(2) refining step: taking extract solution and pass through macroporous resin chromatography, the ethanol with 50% carries out eluting, collects eluent, is evaporated to do, obtains Calyx Seu Fructus Physalis total steroidal extract.
Extraction the most according to claim 1 and the method for the total steroidal of refined Calyx Seu Fructus Physalis, it is characterised in that during extraction, extracting method is any one of ultrasonic method, circumfluence method and cold-maceration.
Extraction the most according to claim 1 and the method for the total steroidal of refined Calyx Seu Fructus Physalis, it is characterized in that: described extracting method uses ethanol reflux extraction, i.e. add 50% ~ 95% concentration ethanol of dry Calyx Seu Fructus Physalis weight 20 ~ 60 times amount, heating and refluxing extraction 1 ~ 4 time, time used by every time extracting is 0.5 ~ 3 hour, sucking filtration while hot, merging filtrate, recovery filtrate is to without alcohol, and water liquid is concentrated into paste and obtains extractum.
Extraction the most according to claim 3 and the method for the total steroidal of refined Calyx Seu Fructus Physalis, it is characterized in that: described ethanol reflux extraction, i.e. add 70% concentration ethanol of dry Calyx Seu Fructus Physalis weight 30 times amount, heating and refluxing extraction 2 times, time used by every time extracting is 2 hours, sucking filtration while hot, merging filtrate, recovery filtrate is to without alcohol, and water liquid is concentrated into paste and obtains extractum.
Extraction the most according to claim 1 and the method for the total steroidal of refined Calyx Seu Fructus Physalis, it is characterised in that time refined, any one of HPD-100A, HPD-200B, HPD-700, DM130, HPD-500, D-101 and AB-8 type macroporous resin selected by resin.
Extraction the most according to claim 1 and the method for the total steroidal of refined Calyx Seu Fructus Physalis, when it is characterized in that refined, with AB-8 type macroporous resin, take the extract concentration of 4.196 ~ 16.675mg/ml, adsorb 0.5 ~ 6 hour, using 50% ~ 95% concentration ethanol as eluant, coutroi velocity is 1 ~ 4BV/h, collect the eluent of 0.1 ~ 0.9BV, be evaporated to do, obtain Calyx Seu Fructus Physalis total steroidal extract.
Extraction the most according to claim 6 and the method for the total steroidal of refined Calyx Seu Fructus Physalis, when it is characterized in that refined, take the Calyx Seu Fructus Physalis extracting solution that concentration is 8.338mg/ml ~ 16.675mg/ml, slow transitting through the AB-8 type macroporous resin chromatography of pretreatment with 1BV/h flow velocity, adsorb 1h, the ethanol with 50% carries out eluting, flow velocity is 2BV/h, collect the eluent between 0.3 ~ 0.8BV, be evaporated to do, obtain Calyx Seu Fructus Physalis total steroidal extract.
Calyx Seu Fructus Physalis total steroidal extract prepared by Hydrolysis kinetics method the most according to claim 1, i.e. Calyx Seu Fructus Physalis anti-pharyngitis effective site.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107050177A (en) * | 2017-02-28 | 2017-08-18 | 吉林大学 | It is a kind of to be used to treat Chinese medicine composition of acne and preparation method thereof |
| CN111713731A (en) * | 2020-06-15 | 2020-09-29 | 深圳烟草工业有限责任公司 | Preparation method and application of calyx seu fructus physalis extract |
| CN113583470A (en) * | 2021-07-31 | 2021-11-02 | 彼悦(北京)科技有限公司 | Multifunctional composite plant dye and preparation method and application thereof |
| CN113750157A (en) * | 2021-10-12 | 2021-12-07 | 中国药科大学 | Preparation method of calyx seu fructus physalis extract, calyx seu fructus physalis extract and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1742875A (en) * | 2005-09-28 | 2006-03-08 | 吉林大学 | Anti-inflammation medicine of Jindenglong on changbaishan mountain and preparation method |
| CN1899501A (en) * | 2006-07-03 | 2007-01-24 | 邱书奇 | Chinese medicine compound preparation for treating laryngopharyngitis and tonsillitis and its preparing method |
| CN102885937A (en) * | 2012-09-29 | 2013-01-23 | 济南星懿医药技术有限公司 | Preparation method and application of morelberry effective fractions |
| CN103933209A (en) * | 2014-04-03 | 2014-07-23 | 沈阳化工大学 | Method for extracting steroid saponin from Chinese lantern-plant stems and persistent calyx |
-
2015
- 2015-01-08 CN CN201510008149.9A patent/CN105816569A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1742875A (en) * | 2005-09-28 | 2006-03-08 | 吉林大学 | Anti-inflammation medicine of Jindenglong on changbaishan mountain and preparation method |
| CN1899501A (en) * | 2006-07-03 | 2007-01-24 | 邱书奇 | Chinese medicine compound preparation for treating laryngopharyngitis and tonsillitis and its preparing method |
| CN102885937A (en) * | 2012-09-29 | 2013-01-23 | 济南星懿医药技术有限公司 | Preparation method and application of morelberry effective fractions |
| CN103933209A (en) * | 2014-04-03 | 2014-07-23 | 沈阳化工大学 | Method for extracting steroid saponin from Chinese lantern-plant stems and persistent calyx |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107050177A (en) * | 2017-02-28 | 2017-08-18 | 吉林大学 | It is a kind of to be used to treat Chinese medicine composition of acne and preparation method thereof |
| CN111713731A (en) * | 2020-06-15 | 2020-09-29 | 深圳烟草工业有限责任公司 | Preparation method and application of calyx seu fructus physalis extract |
| CN113583470A (en) * | 2021-07-31 | 2021-11-02 | 彼悦(北京)科技有限公司 | Multifunctional composite plant dye and preparation method and application thereof |
| CN113583470B (en) * | 2021-07-31 | 2023-05-23 | 彼悦(北京)科技有限公司 | Multifunctional composite vegetable dye and preparation method and application thereof |
| CN113750157A (en) * | 2021-10-12 | 2021-12-07 | 中国药科大学 | Preparation method of calyx seu fructus physalis extract, calyx seu fructus physalis extract and application thereof |
| CN113750157B (en) * | 2021-10-12 | 2022-07-29 | 中国药科大学 | Preparation method of calyx seu fructus physalis extract, calyx seu fructus physalis extract and application thereof |
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