CN107050177A - It is a kind of to be used to treat Chinese medicine composition of acne and preparation method thereof - Google Patents
It is a kind of to be used to treat Chinese medicine composition of acne and preparation method thereof Download PDFInfo
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Abstract
The present invention provides a kind of for treating Chinese medicine composition of acne and preparation method thereof, belongs to technical field of traditional Chinese medicine preparation method.The Chinese medicine composition in parts by weight, including following raw material：5 15 parts of Herba Xanthii extract, 15 30 parts of Caulis Mahoniae extract, 10 30 parts of Ajuga nippanensis extract, 10 30 parts of Calyx Seu Fructus Physalis extract, 15 30 parts of gumbo flower extract, 10 30 parts of dandelion extract, 10 30 parts of Honegsukle flower P.E, 5 25 parts of Radix Arnebiae extract.The present invention also provides a kind of preparation method for being used to treat the Chinese medicine composition of acne.The Chinese medicine composition is to be used as main component using natural Chinese medicinal herb Siberian cocklebur grass, japanese bugle herb, wintercherry fruit or calyx, okra flowers, follow the principle of medication of Chinese medicine " monarch ", obtained Chinese medicine composition can be granule, water-honeyed pill agent, capsule and exterior-applied gel, for treatment acne effect significantly, have no toxic side effect, no dependence and uneasy to recur.
Description
Technical field
The invention belongs to technical field of traditional Chinese medicine preparation method, and in particular to it is a kind of be used for treat acne Chinese medicine composition and
Its preparation method.
Background technology
Acne is disease of skin caused by multifactor interaction, and its morbidity is mainly a large amount of with sex hormone level, sebaceous glands
Secretion, propionibacterium acnes hyper-proliferative, the factor such as the dyskeratosis and inflammation of pilosebaceous duct are relevant.Environmental factor with
Inherent cause influences each other, and mutually promotes, and induces and has aggravated acne.Clinical manifestation is face, neck, the acne at back, mound
Rash, warts, tubercle, tumour, severe patient leave Acne Scar and pigment deposition spot, have impact on the study, work, interpersonal friendship of patient
Past and social life, is decreased obviously the quality of life of patient.Tetracyclines, macrolide are commonly used in treatment of the doctor trained in Western medicine to acne
Some antibiotics such as class, sulfamido, therapeutic effect is general, and long-term taking is larger to the toxicity of liver kidney and easily recurs,
Easy resistance, causes course of disease recurrent exerbation, patient is difficult to receive.
Modern TCM thinks that patients with acne is vigorous because of puberty life, and vim and vigour are abundant, and sun heat is partially prosperous, and excess heat hinders lung, lung heat
Stifling, accumulateing resistance skin can cause wind and heat in the lung meridian to demonstrate,prove；Unhygienic diet, partially thermophilic pungent delicious food, the product of greasy raw meat hair are burnt in damp and hot tired resistance,
Follow and steamed through upper, cause stomach excess fire card；The heart channel of Hang-Shaoyin latent heat, is loved without going, and internal heat, which permits, contains, and rushes face in accumulated heat and fire of making up one's mind fills Sheng card；
Edgy, or feelings will is smooth, causes liver dysfunction, liver depression is then sick, strongly fragrant longization fire, or overfeeding greasy and surfeit flavour, then raw wet to help heat, liver
Strongly fragrant to be coexisted with damp and hot, outer general skin forms syndrome of dampness-heat of liver channel；The deficiency of the kidney yin cause kidney imbalance between YIN and YANG, mutually fire it is partially prosperous, follow through
It is upper steaming and send out the deficiency of the kidney yin card；Spleen insufficiency, melancholy,anxiety and spleen, retention of water-damp in the body is wet to be polymerized to phlegm, with the passing of time changes heat, and damp and hot folder phlegm condenses
Skin causes phlegm wet to condense card；Disease is not cured long, and heresy is poly- not to be dissipated, and stagnation of the circulation of vital energy blood addiction, passages through which vital energy circulates is lost freely, or rheumatism heat evil is accumulate in skin, qi and blood
Held back, cohesion forms the tired stagnant card of qi and blood.Therefore in the treatment with removing damp-heat, removing toxic substances activating blood circulation method composition《Li Wen》Say：" it is controlled outside
Reason, i.e., in the reason controlled, the medicine controlled outside, i.e., in the medicine controlled, it is clinical severe acne must be coordinated outside control, control outside with it is interior control simply to
Medicine approach is different, and its medical knowledge drug effect is identical, and outer control can make the through focus of medicine and play therapeutic action, reduce liver
First pass effect, safe without toxic side effect.It is used although having had some Chinese medicine preparations in terms of acne is treated at present
The taste number of prescription Chinese medicine is excessive, and is many water decoctions, without purification removal of impurities, and curative effect is nor highly desirable, so far not yet
There is the medicine that can effect a radical cure acne.
The content of the invention
It is an object of the invention to provide a kind of Chinese medicine composition for being used to treat acne and preparation method thereof, the Chinese traditional medicine composition
Thing does not have any side effect, it is orally available can external application, therapeutic effect is notable.
Present invention firstly provides a kind of Chinese medicine composition for being used to treat acne, the Chinese medicine composition is according to parts by weight
Meter, including following raw material：
5-15 parts of Herba Xanthii extract, 15-30 parts of Caulis Mahoniae extract, 10-30 parts of Ajuga nippanensis extract, wintercherry fruit or calyx is carried
Take 10-30 parts of thing, 15-30 parts of gumbo flower extract, 10-30 parts of dandelion extract, 10-30 parts of Honegsukle flower P.E, Asian puccoon
5-25 parts of extract.
Preferably, the Chinese medicine composition in parts by weight, including following raw material：
6-10 parts of Herba Xanthii extract, 20-25 parts of Caulis Mahoniae extract, 15-20 parts of Ajuga nippanensis extract, wintercherry fruit or calyx is carried
Take 15-25 parts of thing, 20-25 parts of gumbo flower extract, 15-25 parts of dandelion extract, 15-25 parts of Honegsukle flower P.E, Asian puccoon
10-15 parts of extract.
Preferably, the Chinese medicine composition in parts by weight, including following raw material：
8 parts of Herba Xanthii extract, 22 parts of Caulis Mahoniae extract, 18 parts of Ajuga nippanensis extract, 20 parts of Calyx Seu Fructus Physalis extract,
22 parts of gumbo flower extract, 20 parts of dandelion extract, 20 parts of Honegsukle flower P.E, 12 parts of Radix Arnebiae extract.
The present invention also provides a kind of preparation method for being used to treat the Chinese medicine composition of acne, and this method includes：
Step one：It is prepared by Herba Xanthii extract
Siberian cocklebur grass is cleaned, after oven drying crush, stayed overnight with the distilled water immersion of 6-8 times of crude drug amount, temperature 50 C-
It is heated to reflux at 70 DEG C 0.5-2.5 hours, in triplicate, merges extract solution, be concentrated under reduced pressure, is added ethanol and stir, stand,
By contaminant filter, supernatant is concentrated under reduced pressure to obtain Herba Xanthii extract；
Step 2：It is prepared by Caulis Mahoniae extract
Leatherleaf mahonia is cleaned, crushed after oven drying, with the distilled water refluxing extraction of 6-8 times of crude drug amount, temperature 50 C-
Heating extraction 0.5-2.5 hours at 70 DEG C, in triplicate, merge extract solution, be concentrated under reduced pressure, D-101 types macropore tree on concentrate
Fat post, goes the removal of impurity, with elution, collects eluent, Caulis Mahoniae extract must be obtained by being concentrated under reduced pressure；
Step 3：It is prepared by Ajuga nippanensis extract
Japanese bugle herb is extracted using 70% ethanol, soaked overnight, heating water bath, refluxing extraction three times, described extraction
Temperature is 50 DEG C -80 DEG C, and the single-trial extraction time is 0.5-2.5 hours, merges extract solution, is concentrated, filtering, D-101 on concentrate
Type macroporous resin column, goes the removal of impurity, then with volume fraction is respectively 10%, 30%, 50% ethanol gradient elution, takes 50% to wash
De- part reduced pressure obtains Ajuga nippanensis extract.
Step 4：It is prepared by Calyx Seu Fructus Physalis extract
Wintercherry fruit or calyx is cleaned, crushed after being dried, using 85% ethanol, solid-liquid ratio 1:5-1:8, heating water bath, refluxing extraction
Three times, Extracting temperature is 50 DEG C -80 DEG C, each 0.5-2.5 hours, merges extract solution, is concentrated, filtering, then big through D-101-I types
Hole resin adsorption, collects 50% and elutes the position oven drying that is concentrated under reduced pressure and produce Calyx Seu Fructus Physalis extract；
Step 5：It is prepared by gumbo flower extract
Okra flowers are cleaned, crushed after being dried, using 85% ethanol, solid-liquid ratio 1:5-1:8, heating water bath, refluxing extraction
Three times, Extracting temperature is 70 DEG C -80 DEG C, each 0.5-2.5 hours, merges extract solution, is concentrated, filtering, then through D-108 type macropores
Resin adsorption, collects 60% and elutes the position oven drying that is concentrated under reduced pressure and produce gumbo flower extract；
Step 6：Mixing
By Herba Xanthii extract, Caulis Mahoniae extract, Ajuga nippanensis extract, Calyx Seu Fructus Physalis extract, gumbo flower extract,
Dandelion extract, Honegsukle flower P.E and Radix Arnebiae extract mixing, cross 60-80 mesh sieves after dry, pulverize at 70 DEG C -85 DEG C,
Obtain the Chinese medicine composition for treating acne.
Preferably, the ethanol mass fraction of described step one is 60-80%.
Preferably, the time of repose of described step one is 1-3h.
Preferably, described step two eluent is hydrochloric acid solution.
Preferably, the concentration of described step two hydrochloric acid solution is 0.04-0.08mol/L.
Preferably, the mass ratio of described japanese bugle herb and ethanol is 1:3-1:8.
The Chinese medicine composition of the present invention can add conventional pharmaceutic adjuvant and pharmaceutically acceptable oral formulations are made such as
Granule, water-honeyed pill agent, capsule and external preparation gel.And the tablet, capsule or granule and external-use gel
Agent can be made using the customary preparation methods of corresponding species preparation.
The pharmacological action of the present invention is as follows：
1. Siberian cocklebur grass is composite family cocklebur Siberian cocklebur Xanthium sibiricum Patrin. stem, leaf, portion of being used as medicine
It is divided into ground drying nest, China's pharmacopeia records Siberian cocklebur X.strumarium fruit " achene of Siberian cocklebur " hyoscine, for treating wind
Cold headache, nasosinusitis runny nose, it is nose section common medicine based on rubella itch disease.It is used for diuresis in Siberian cocklebur different parts among the people, urges
Tell, urge and letting out, treatment prostatic disorders, hepatitis, pruitus and leprosy etc., especially Siberian cocklebur grass toxicity is smaller, this medicine is mild,
Therefore with clearing away wind heat, the solution effect such as wet poison, to skin eczema, pruitus, leprosy, deep abscess, sore it is evident in efficacy.
The chemical composition of Siberian cocklebur grass mainly contains volatile oil, phenolic acid, sesquialter terpene lactone, and (Xanthatin has significant anti-Staphylococcus aureus
Character includes Methicillin-resistant Staphylococcus aureus, it may have cytotoxic activity.) modern pharmacological research find liposoluble ingredient have it is good
The bioactivity such as well anti-oxidant, anti-inflammatory, antimicrobial, enzyme inhibition, protection liver cell, suppression platelet aggregation.This
Invention Siberian cocklebur grass used, which is used as medicine, partly includes under ground portion.
2. leatherleaf mahonia is Berberidaceae plant mahonia bealei Mahonia bealei (Fort) Carr. drying stem.Its
Bitter, tremble with fear, with heat-clearing and damp-drying drug, purging intense heat and detonicating, enriching yin support lung the effect of, cure mainly damp-heat dysentery, jaundice, red eye, swell pain, boil,
Carbuncle swells.The main chemical composition of leatherleaf mahonia is alkaloids, flavone compound, and its contained alkaloids Berberine is to a variety of
Bacterium (staphylococcus aureus, chlosma etc.) has preferable bacteriostatic activity.Modern pharmacological research finds that leatherleaf mahonia has
The pharmacological activity such as anticancer, antibacterial, anti-inflammatory, antiviral, anti-arrhythmia, hypoglycemic, antitumor.
3. japanese bugle herb (Ajuga nipponensis Makino) be Labiatae bugle, all herbal medicine, again
Name ajuga decumbens, ajuga nipponensis Makino, agar, the effect of being relieved the pain with clearing heat and detoxicating, cool blood dissipates the stasis of blood, anti-inflammation detumescence is coughed to lung heat
Blood, abscess of throat, acute mastitis, acute appendicitis, boil poisoning, traumatism and bleeding, scald, the disease such as bite has significant curative effect.Its chemistry into
Point contain plant ecdysterone class, flavonoids, Diterpenes, iridoids, pharmacological research be proved plant ecdysterone class into
Point have promote protein synthesis, adjust glycometabolism, immunological regulation and influence central cholinergic neurotransmitter metabolism etc. many-side
Pharmacological activity, with promote Traumatic wound healing effect.Flavonoids is mainly luteolin (cyanidenon) to golden yellow
Staphylococcus, alpha streptococcus, micrococcus catarrhalis, pneumococcus, Pseudomonas aeruginosa have certain bacteriostasis.
4. wintercherry fruit or calyx is that Solanaceae [Physalis alkekengi L.var.franchetii (Mast.) Makino] is eggplant
The drying place calyx of section's monkey flower or the place calyx with fruit.Alias franchet groundcherry persistent calyx or fruit, red Miss, Chinese lantern etc..Wintercherry fruit or calyx bitter,
Acid, it is cold in nature.Have clearing heat and detoxicating, relieving sore-throat, resolving sputum, inducing diuresis for treating strangurtia, etc. effect, for mute, phlegm-heat cough for the treatment of pharyngalgia sound, urine is not
Profit, puckery pain of heat gonorrhea；Control pemphigus, eczema etc. outside.It (is mainly physagulin class, new wintercherry that its chemical composition, which includes steroid,
Bitter principle class), flavonoids, sterols, alkaloids, carotenoid, amino acids, volatile oil, polysaccharide etc..Modern medicine
Pharmacological research, which is proved wintercherry fruit or calyx, the effect such as antibacterial, anti-inflammatory, antiviral, anti-oxidant, analgesia use, diuresis.
5. okra flowers are the drying corolla of malvaceae plant Sunset Abelmoschus Root (Abelmoschus manihotL.Medic.), again
Name gumbo, ambrette etc..Its is sweet, trembles with fear, and with clearing heat and detoxicating, inducing diuresis for removing edema, for swollen ulcer drug, scald, the medicine begins to carry
In《Jiayou Bencao》；《Compendium of Materia Medica》Say：Sunset abelmoschus flower, it is spent smell sweet, cold, sliding, nontoxic, controls all malignant sore pus and be not recovered long
Person, what work end was applied is cured, and is persons particularly liable to develop skin infection key medicine person.Its chemical composition mainly containing flavonoids, organic acid, steroid and volatility into
Point.Modern clinic pharmacodynamic study sunset abelmoschus flower has anti-infective, anti-inflammatory, suppresses platelet aggregation, anti thrombotic action, suppresses second
The effect such as kidney is protected in hepatovirus, liver protection.
6. dandelion (Taraxacum mongolicum Hand.Mazz) is feverfew (Compositate) dandelion
Belong to (Taraxacum F.H.Wigg.) plant, bitter is sweet, cold in nature, tool and clearing heat and detoxicating, dispersing swelling and dissipating binds, the work(of inducing diuresis for treating strangurtia
Can, for furuncle swelling toxin, warm jaundice, puckery pain of heat gonorrhea.Its chemical composition has flavonoids, Sesquiterpene lactones, Coumarins, three
Terpene, phytosterol, phenolic acid class, carotene class, volatile oil compound are given birth in addition with a variety of aliphatic acid, choline, dimension
Element, mineral matter, pectin etc., pharmacological research prove dandelion have antibacterial, antitumor, anti-oxidant, anti-inflammatory, antiallergy, antithrombotic,
Diuresis etc. is acted on.
7. honeysuckle is the dry flower of caprifoliaceae plant honeysuckle (Lonicera Japonica) or the flower just opened, and has clear
Thermal detoxification, wind-heat dissipating, for carbuncle swells furuncle sore, larynx numbness, erysipelas, toxic-heat and blood stasis, warm disease heating.Its chemistry point mainly has, flavones
Class, organic acid, triterpenes and iridoid, volatile oil compound.It is antibacterial, disease-resistant that pharmacological research shows that honeysuckle has
Malicious, antipyretic, anti-inflammatory, lidan liver, hemostasis, anti-oxidant, immunological regulation, the effect of antifertility.
8. Asian puccoon is comfrey lithospermum euchromum Royle Arnebia euchroma (Royle) johnst. dry root.History is carried
In《Sheng Nong's herbal classic》, bitter, it is cold in nature, with removing pattogenic heat from the blood and toxic material from the body promoting eruption, the effect of change, for blood-heat and toxin exuberance, macula, measles not
Thoroughly, sore rash eczema.Groomwell root oil be from Asian puccoon extract and obtain, have wound healing, suppress cicatrization, moisten skin, resist
Scorching, antioxidation.Its chemical composition mainly have volatilization class, naphthoquinones class, flavonoids, phenolic acid class, steroidal and terpene, lignin,
The compounds such as alkaloid.Pharmacological research show Asian puccoon there is anti-inflammatory, it is antibacterial, anticancer, antiviral, liver protection, hemostasis, vasodilatory
Effect.
Beneficial effects of the present invention
Present invention firstly provides a kind of Chinese medicine composition for being used to treat acne, the Chinese medicine composition is according to parts by weight
Meter, including following raw material：5-15 parts of Herba Xanthii extract, 15-30 parts of Caulis Mahoniae extract, 10-30 parts of Ajuga nippanensis extract,
10-30 parts of Calyx Seu Fructus Physalis extract, 15-30 parts of gumbo flower extract, 10-30 parts of dandelion extract, Honegsukle flower P.E 10-30
Part, 5-25 parts of Radix Arnebiae extract.The Chinese medicine composition is made with natural Chinese medicinal herb Siberian cocklebur grass, japanese bugle herb, wintercherry fruit or calyx, okra flowers
For main component, it then follows the principle of medication of Chinese medicine " monarch ", obtained Chinese medicine composition can be granule, water-honeyed pill
Agent, capsule and exterior-applied gel, for treatment acne (whelk) effect significantly, have no toxic side effect, no dependence and are difficult
Recurrence.
The present invention also provides a kind of preparation method for being used to treat the Chinese medicine composition of acne, and the preparation method is simple, former
Material is easy to get, and obtaining traditional Chinese medicine composition for treating acne has remarkable result.
Embodiment
With reference to specific embodiment, the present invention will be further described in detail, and the dandelion being related in embodiment carries
It is commercially available acquisition to take thing, Honegsukle flower P.E and Radix Arnebiae extract.
A kind of preparation for being used to treat the Chinese medicinal composition granules of acne of embodiment 1
Raw material (g) is weighed by following weight：
Herba Xanthii extract 5g, Caulis Mahoniae extract 15g, Ajuga nippanensis extract 10g, Calyx Seu Fructus Physalis extract 10g, gumbo
Flower extract 15g, dandelion extract 10g, Honegsukle flower P.E 10g, Radix Arnebiae extract 5g.
Step one：It is prepared by Herba Xanthii extract
35g Siberian cocklebur grass is cleaned, crushes, is stayed overnight with the distilled water immersion of 6 times of crude drug amounts, in temperature 50 C after oven drying
Under be heated to reflux 2.5 hours, in triplicate, merge extract solution, be concentrated under reduced pressure, add 60-80% ethanol and stir, stand 1-
3h, by contaminant filter, supernatant is concentrated under reduced pressure, and oven drying obtains Herba Xanthii extract；
Step 2：It is prepared by Caulis Mahoniae extract
225g leatherleaf mahonias are cleaned, crushed after oven drying, with the distilled water refluxing extraction of 6 times of crude drug amounts, in temperature 50
Heating extraction 2.5 hours at DEG C, in triplicate, merge extract solution, are concentrated under reduced pressure, and D-101 types macroporous resin column on concentrate is used
The water-solubility impurities such as distilled water flushing protein, the polysaccharide of three times volume, with the 0.04mol/L hydrochloric acid solutions of 8 times of resin volumes
Elution, collects extract solution, is concentrated under reduced pressure, oven drying obtains Caulis Mahoniae extract the most；
Step 3：It is prepared by Ajuga nippanensis extract
100g japanese bugle herbs are extracted using 70% ethanol, solid-liquid ratio is 1:3 soaked overnights, heating water bath, refluxing extraction
Three times, described Extracting temperature is 50 DEG C, and the single-trial extraction time is 2.5 hours, merges extract solution, is concentrated, filtering, on concentrate
D-101 type macroporous resin columns, with water-solubility impurities such as distilled water flushing protein, the polysaccharide of three times volume, then use volume fraction
Respectively 10%, 30%, 50% ethanol gradient elution, take 50% elution fraction to depressurize, oven drying obtains japanese bugle herb and carry
Take thing.
Step 4：It is prepared by Calyx Seu Fructus Physalis extract
100g wintercherry fruit or calyxs are cleaned, crushed after being dried, using 85% ethanol, solid-liquid ratio 1:5, heating water bath, refluxing extraction
Three times, Extracting temperature is 50 DEG C, 2.5 hours every time, merges extract solution, concentrates, filtering, then is inhaled through D-101-I types macroreticular resin
It is attached, collect 50% and elute the position oven drying that is concentrated under reduced pressure and produce Calyx Seu Fructus Physalis extract；
Step 5：It is prepared by gumbo flower extract
75g okra flowers are cleaned, crushed after being dried, using 85% ethanol, solid-liquid ratio 1:5-1:8, heating water bath, backflow is carried
Take three times, Extracting temperature is 50 DEG C, 2.5 hours every time, merges extract solution, concentrates, filtering, then through D-101-I type macroreticular resins
Absorption, collects 50% elution position and is concentrated under reduced pressure, oven drying produces gumbo flower extract；
Step 6：Mixing
Said extracted thing is well mixed with commercially available dandelion extract, Honegsukle flower P.E and Radix Arnebiae extract, powder
60-80 mesh sieves are crossed after broken, by 1:1 ratio (Chinese medicine composition of the present invention：Powdered glucose) add dried powdered glucose, stirring
Uniformly, add 50% ethanol and softwood (that holds is agglomerating, and light pressure dissipates) is made is put in 16 mesh sieves and prepare 70 DEG C of dryings of particle, then through 14
After mesh sieve whole grain, the Chinese medicinal composition granules for treating acne are can be made into.
A kind of preparation for being used to treat the traditional Chinese medicine composition water honey pill agent of acne of embodiment 2
Raw material (g) is weighed by following weight：
Herba Xanthii extract 15g, Caulis Mahoniae extract 30g, Ajuga nippanensis extract 30g, Calyx Seu Fructus Physalis extract 30g, autumn
Sunflower extract 30g, dandelion extract 30g, Honegsukle flower P.E 30g, Radix Arnebiae extract 25g.
Step one：It is prepared by Herba Xanthii extract
105g Siberian cocklebur grass is cleaned, crushes, is stayed overnight with the distilled water immersion of 8 times of crude drug amounts, in temperature 70 after oven drying
It is heated to reflux at DEG C 0.5 hour, in triplicate, merges extract solution, be concentrated under reduced pressure, is added 80% ethanol and stir, stand 3h,
By contaminant filter, supernatant is concentrated under reduced pressure, and oven drying obtains Herba Xanthii extract；
Step 2：It is prepared by Caulis Mahoniae extract
450g leatherleaf mahonias are cleaned, crushed after oven drying, with the distilled water refluxing extraction of 8 times of crude drug amounts, in temperature 70
Heating extraction 2.5 hours at DEG C, in triplicate, merge extract solution, are concentrated under reduced pressure, and D-101 types macroporous resin column on concentrate is used
The water-solubility impurities such as distilled water flushing protein, the polysaccharide of three times volume.With the 0.08mol/L hydrochloric acid solutions of 8 times of resin volumes
Elution, collects extract solution, is concentrated under reduced pressure, oven drying obtains Caulis Mahoniae extract the most；
Step 3：It is prepared by Ajuga nippanensis extract
300g japanese bugle herbs are extracted using 70% ethanol, solid-liquid ratio is 1:8 soaked overnights, heating water bath, refluxing extraction
Three times, described Extracting temperature is 80 DEG C, and the single-trial extraction time is 0.5 hour, merges extract solution, is concentrated, filtering, on concentrate
D-101 type macroporous resin columns, with water-solubility impurities such as distilled water flushing protein, the polysaccharide of three times volume, then use volume fraction
Respectively 10%, 30%, 50% ethanol gradient elution, take 50% elution fraction to depressurize, oven drying obtains japanese bugle herb and carry
Take thing.
Step 4：It is prepared by Calyx Seu Fructus Physalis extract
300g wintercherry fruit or calyxs are cleaned, crushed after being dried, using 85% ethanol, solid-liquid ratio 1:8, heating water bath, refluxing extraction
Three times, Extracting temperature is 70 DEG C, 0.5 hour every time, merges extract solution, concentrates, filtering, then is inhaled through D-101-I types macroreticular resin
It is attached, collect 50% elution position and be concentrated under reduced pressure, oven drying produces Calyx Seu Fructus Physalis extract；
Step 5：It is prepared by gumbo flower extract
150g okra flowers are cleaned, crushed after being dried, using 85% ethanol, solid-liquid ratio 1:8, heating water bath, refluxing extraction
Three times, Extracting temperature is 70 DEG C, 0.5 hour every time, merges extract solution, concentrates, filtering, then is inhaled through D-101-I types macroreticular resin
It is attached, collect 50% elution position and be concentrated under reduced pressure, oven drying produces gumbo flower extract；
Step 6：Mixing
Said extracted thing is well mixed with commercially available dandelion extract, Honegsukle flower P.E and Radix Arnebiae extract, powder
It is broken to cross 80-100 mesh sieves；Honey is endured into boiling with high heat, slow fire is switched at once and is endured slowly, it is in light red that raw honey heating is endured into boiling to foam
Gloss；Composition and the refining refined is close by 1:The uniform quality mixing of 1 ratio, stands, and medicinal powder is mixed with honey and moistens,
Ball be can be made on after toughness.Upper air dry oven is dried, and produces the traditional Chinese medicine composition water honey pill agent for treating acne.
A kind of preparation for being used to treat the Chinese medicinal composition capsules agent of acne of embodiment 3
Raw material (g) is weighed by following weight：
Herba Xanthii extract 6g, Caulis Mahoniae extract 20g, Ajuga nippanensis extract 15g, Calyx Seu Fructus Physalis extract 15g, gumbo
Flower extract 20g, dandelion extract 15g, Honegsukle flower P.E 15g, Radix Arnebiae extract 10g.
Step one：It is prepared by Herba Xanthii extract
42g Siberian cocklebur grass is cleaned, crushes, is stayed overnight with the distilled water immersion of 6 times of crude drug amounts after oven drying, in 55 DEG C of temperature
Under be heated to reflux 1 hour, in triplicate, merge extract solution, be concentrated under reduced pressure, add 70% ethanol and stir, stand 2h, will be miscellaneous
Matter is filtered, and supernatant is concentrated under reduced pressure, and oven drying obtains Herba Xanthii extract；
Step 2：It is prepared by Caulis Mahoniae extract
300g leatherleaf mahonias are cleaned, crushed after oven drying, with the distilled water refluxing extraction of 6 times of crude drug amounts, in temperature 55
Heating extraction 1 hour at DEG C, in triplicate, merges extract solution, is concentrated under reduced pressure, D-101 types macroporous resin column on concentrate, with three
The water-solubility impurities such as distilled water flushing protein, the polysaccharide of times volume.With the 0.05mol/L hydrochloric acid solutions of 8 times of resin volumes most
For elution, extract solution is collected, is concentrated under reduced pressure, oven drying obtains Caulis Mahoniae extract；
Step 3：It is prepared by Ajuga nippanensis extract
150g japanese bugle herbs are extracted using 70% ethanol, solid-liquid ratio is 1:4 soaked overnights, heating water bath, refluxing extraction
Three times, described Extracting temperature is 55 DEG C, and the single-trial extraction time is 1 hour, merges extract solution, is concentrated, filtering, D- on concentrate
101 type macroporous resin columns, with water-solubility impurities such as distilled water flushing protein, the polysaccharide of three times volume, then with volume fraction point
Not Wei 10%, 30%, 50% ethanol gradient elution, take 50% elution fraction depressurize, oven drying obtain japanese bugle herb extraction
Thing.
Step 4：It is prepared by Calyx Seu Fructus Physalis extract
150g wintercherry fruit or calyxs are cleaned, crushed after being dried, using 85% ethanol, solid-liquid ratio 1:6, heating water bath, refluxing extraction
Three times, Extracting temperature is 80 DEG C, 1 hour every time, merges extract solution, concentrates, filtering, then through D-101 type macroporous resin adsorptions, is received
Collection 50% elutes position and is concentrated under reduced pressure, and oven drying produces Calyx Seu Fructus Physalis extract；
Step 5：It is prepared by gumbo flower extract
100g okra flowers are cleaned, crushed after being dried, using 85% ethanol, solid-liquid ratio 1:6, heating water bath, refluxing extraction
Three times, Extracting temperature is 80 DEG C, 1 hour every time, merges extract solution, concentrates, filtering, then through D-108 type macroporous resin adsorptions, is received
Collection 50% elutes position and is concentrated under reduced pressure, and oven drying produces gumbo flower extract；
Step 6：Mixing
Said extracted thing is well mixed with commercially available dandelion extract, Honegsukle flower P.E and Radix Arnebiae extract, powder
After broken, capsule is filled, the Chinese medicinal composition capsules agent for treating acne is made.
A kind of preparation for being used to treat the Chinese medicine composition exterior-applied gel of acne of embodiment 4
Raw material (g) is weighed by following weight：
Herba Xanthii extract 10g, Caulis Mahoniae extract 15g, Ajuga nippanensis extract 10g, Calyx Seu Fructus Physalis extract 25g, autumn
Sunflower extract 15g, dandelion extract 10g, Honegsukle flower P.E 10g, Radix Arnebiae extract 5g.
Step one：It is prepared by Herba Xanthii extract
70g Siberian cocklebur grass is cleaned, crushes, is stayed overnight with the distilled water immersion of 8 times of crude drug amounts, in temperature 65 after oven drying
It is heated to reflux at DEG C 2 hours, in triplicate, merges extract solution, be concentrated under reduced pressure, is added 80% ethanol and stir, stand 2h, will
Contaminant filter, the supernatant oven drying that is concentrated under reduced pressure obtains Herba Xanthii extract；
Step 2：It is prepared by Caulis Mahoniae extract
225g leatherleaf mahonias are cleaned, crushed after oven drying, with the distilled water refluxing extraction of 6-8 times of crude drug amount, in temperature
Heating extraction 2 hours at 65 DEG C, in triplicate, merge extract solution, are concentrated under reduced pressure, and D-101 types macroporous resin column on concentrate is used
The water-solubility impurities such as distilled water flushing protein, the polysaccharide of three times volume.With the 0.07mol/L hydrochloric acid solutions of 8 times of resin volumes
Elution, collects extract solution, is concentrated under reduced pressure, oven drying obtains Caulis Mahoniae extract the most；
Step 3：It is prepared by Ajuga nippanensis extract
100g japanese bugle herbs are extracted using 70% ethanol, solid-liquid ratio is 1:4 soaked overnights, heating water bath, refluxing extraction
Three times, described Extracting temperature is 65 DEG C, and the single-trial extraction time is 2 hours, merges extract solution, is concentrated, filtering, D- on concentrate
101 type macroporous resin columns, with water-solubility impurities such as distilled water flushing protein, the polysaccharide of three times volume, then with volume fraction point
Not Wei 10%, 30%, 50% ethanol gradient elution, take 50% elution fraction to be concentrated under reduced pressure, oven drying obtains japanese bugle herb
Extract.
Step 4：It is prepared by Calyx Seu Fructus Physalis extract
250g wintercherry fruit or calyxs are cleaned, crushed after being dried, using 85% ethanol, solid-liquid ratio 1:7, heating water bath, refluxing extraction
Three times, Extracting temperature is 65 DEG C, 2 hours every time, merges extract solution, concentrates, filtering, then through D-101-I type macroporous resin adsorptions,
Collect 50% elution position to be concentrated under reduced pressure, oven drying produces Calyx Seu Fructus Physalis extract；
Step 5：It is prepared by gumbo flower extract
75g okra flowers are cleaned, crushed after being dried, using 85% ethanol, solid-liquid ratio 1:7, heating water bath, refluxing extraction three
Secondary, Extracting temperature is 80 DEG C, 2 hours every time, merges extract solution, concentrates, filtering, then through D-108 type macroporous resin adsorptions, is collected
50% elution position is concentrated under reduced pressure, and oven drying produces gumbo flower extract；
Step 6：Mixing
Said extracted thing is well mixed with commercially available dandelion extract, honeysuckle Radix Arnebiae extract, crushed, with 1:
2:0.03 ratio adds distilled water, and ice crystal gel forming agent, electric stirring 5 minutes is stirred, produced again after standing 2 hours
Chinese medicine composition gelata for treating acne.
A kind of preparation for being used to treat the Chinese medicinal composition granules of acne of embodiment 5
Raw material (g) is weighed by following weight：
Herba Xanthii extract 8g, Caulis Mahoniae extract 22g, Ajuga nippanensis extract 18g, Calyx Seu Fructus Physalis extract 20g, gumbo
Flower extract 22g, dandelion extract 20g, Honegsukle flower P.E 20g, Radix Arnebiae extract 12g.
Step one：It is prepared by Herba Xanthii extract
56g Siberian cocklebur grass is cleaned, crushes, is stayed overnight with the distilled water immersion of 7 times of crude drug amounts, in temperature 60 C after oven drying
Under be heated to reflux 1.5 hours, in triplicate, merge extract solution, be concentrated under reduced pressure, add 70% ethanol and stir, stand 2h, will
Contaminant filter, supernatant is concentrated under reduced pressure, and oven drying obtains Herba Xanthii extract；
Step 2：It is prepared by Caulis Mahoniae extract
330g leatherleaf mahonias are cleaned, crushed after oven drying, with the distilled water refluxing extraction of 7 times of crude drug amounts, in temperature 60
Heating extraction 1.5 hours at DEG C, in triplicate, merge extract solution, are concentrated under reduced pressure, and D-101 types macroporous resin column on concentrate is used
The water-solubility impurities such as distilled water flushing protein, the polysaccharide of three times volume.With the 0.06mol/L hydrochloric acid solutions of 8 times of resin volumes
Elution, collects extract solution, is concentrated under reduced pressure, oven drying obtains Caulis Mahoniae extract the most；
Step 3：It is prepared by Ajuga nippanensis extract
180g japanese bugle herbs are extracted using 70% ethanol, solid-liquid ratio is 1:5 soaked overnights, heating water bath, refluxing extraction
Three times, described Extracting temperature is 60 DEG C, and the single-trial extraction time is 1.5 hours, merges extract solution, is concentrated, filtering, on concentrate
D-101 type macroporous resin columns, with water-solubility impurities such as distilled water flushing protein, the polysaccharide of three times volume, then use volume fraction
Respectively 10%, 30%, 50% ethanol gradient elution, take 50% elution fraction to depressurize, oven drying obtains japanese bugle herb and carry
Take thing.
Step 4：It is prepared by Calyx Seu Fructus Physalis extract
200g wintercherry fruit or calyxs are cleaned, crushed after being dried, using 85% ethanol, solid-liquid ratio 1:6, heating water bath, refluxing extraction
Three times, Extracting temperature is 75 DEG C, 1.5 hours every time, merges extract solution, concentrates, filtering, then is inhaled through D-101-I types macroreticular resin
It is attached, collect 50% elution position and be concentrated under reduced pressure, oven drying produces Calyx Seu Fructus Physalis extract；
Step 5：It is prepared by gumbo flower extract
110g okra flowers are cleaned, crushed after being dried, using 85% ethanol, solid-liquid ratio 1:6, heating water bath, refluxing extraction
Three times, Extracting temperature is 75 DEG C, 1.5 hours every time, merges extract solution, concentrates, filtering, then is inhaled through D-101-I types macroreticular resin
It is attached, collect 50% elution position and be concentrated under reduced pressure, oven drying produces gumbo flower extract；
Step 6：Mixing
Said extracted thing is well mixed with commercially available dandelion extract, Honegsukle flower P.E and Radix Arnebiae extract, powder
60-80 mesh sieves are crossed after broken, appropriate dried powdered glucose is added, stirs, 50% ethanol of addition is made softwood and (holds it
Agglomerating, light pressure dissipates) it is put in 16 mesh sieves and prepares 75 DEG C of dryings of particle, then after 14 mesh sieve whole grains, can be made into for treating acne
Chinese medicinal composition granules.
The Chinese medicine composition that embodiment 5 is prepared carries out extracorporeal bacteria inhibitor test, animal toxicity experiment and clinical trial,
Specially：
1st, determined using Odontothrips loti fungistatic effect and Chinese medicine composition of the present invention and metronidazole of the prior art are carried out
Extracorporeal bacteria inhibitor test：
The criterion of In Vitro Bacteriostasis qualitative results：
Antibacterial circle diameter ＜ 8mm are insensitive (-)；Antibacterial circle diameter 8-13mm is less sensitive (+)；Antibacterial circle diameter
13-19mm is medium sensitivity (++)；Antibacterial circle diameter ＞ 19mm are extremely sensitive (+++).The specific experiment result such as institute of table 1 and 2
Show：
The antibacterial circle diameter average value (mm) of three kinds of pathogenic bacteria is contrasted under the Chinese medicine composition various concentrations of the present invention of table 1
Extracorporeal bacteria inhibitor test result of the metronidazole of table 2 to three kinds of pathogenic bacteria
It can be seen from Tables 1 and 2 Chinese medicine composition of the present invention under various concentrations to propionibacterium acnes
ATCC11827, staphylococcus aureus ATCC25923, MRSE ATCC12228 have preferable bacteriostasis.And
Less sensitive is showed to above-mentioned strain as the metronidazole group of control.
2nd, determined using medicine base agar dilution fungistatic effect and extracorporeal bacteria inhibitor test has been carried out to Chinese medicine composition of the present invention：
Experiment is as shown in table 3：
Minimal inhibitory concentration MIC (mg/mL) of the Chinese medicine composition of the present invention of table 3 to three kinds of pathogenic bacteria
Note："-" indicates no growth；"+" represents strain growth
As can be seen from Table 3 propionibacterium acnes, MRSE only it is positive and 40,20,10,5, dosing test tube
In there is bacterium colony to grow, do not grow bacterium colony in remaining test tube, it is known that its MIC is 40mg/mL, and to staphylococcus aureus MIC
For 20mg/mL.
3rd, acute poisoning test
Using kunming mice, SPF grades, each 20 of male and female, body weight is 20 ± 2g, is tested by preclinical medicine institute of Jilin University dynamic
Thing center is provided.Two groups, as every group of 10 male and female half and half, control group and administration group are randomly divided into by body weight.It is normal to raise one
Zhou Hou, mouse fasting 12h before administration；The Cmax that administration group is resistant to animal, maximum volume dose (24g/kg), gavage
Administration 2 times, each 0.6ml/20g is spaced 6 hours.Control group gives equivalent volume sterilized water for injection gavage, routinely raises
Support, Continuous Observation 7 days, and record administration after dead mouse or dying situation, the state of mind, behavioral activity, meal situation,
Fecal character, body weight etc..Test result indicates that：Compared with control group, administration group mouse has no notable difference.Without animal dead,
LD is not measured50.Gross anatomy is carried out to mouse after 7 days, important organ tissue is observed under the microscope and has no swelling and atrophy, face
Color, quality no abnormality seen change.Chinese medicine composition human body recommended intake of the present invention is 9.38g/60kgBW days.
Calculated with average mice body weight 20g, 342 times of Chinese medicine composition maximal tolerance dose of the present invention equivalent to human body recommended amounts.Therefore
Chinese medicine composition acute toxicity of the present invention is low, clinical application safety.
4th, long term toxicity test：
Using Wistar rats, 5 week old, body weight 100g ± 10g, male and female half and half are tested by preclinical medicine institute of Jilin University
Animal center is provided.High dose group, middle dose group, low dose group and control group, every group 10 are randomly divided into by body weight and sex.
Concrete scheme is shown in Table 4.
The Chinese medicine composition of the present invention of table 4 is in various dose group dosage regimen
Observe and monitor general status, food ration, body weight, organ weights, physiochemical indice, the conscience kidney work(of administration group rat
Energy.Experimental result is shown in Table 5 to table 9：
The influence (g) that the Chinese medicine composition of the present invention of table 5 changes to rat body weight
Influence (g/d) of the Chinese medicine composition of the present invention of table 6 to rat food ration
| Time | Blank | High dose | Middle dosage | Low dosage |
| Before administration | 17.30±0.59 | 13.89±1.30 | 13.96±0.77 | 16.22±1.47 |
| D1W | 18.37±1.12 | 14.99±2.03 | 14.67±2.08 | 17.02±1.22 |
| D2W | 21.89±0.93 | 20.22±1.10 | 20.97±0.45 | 21.49±0.35 |
| D3W | 23.71±0.37 | 21.89±2.16 | 22.89±1.66 | 23.89±1.14 |
| D4W | 24.99±0.68 | 23.89±1.14 | 24.03±2.36 | 24.77±0.36 |
| D5W | 28.16±0.22 | 27.33±1.26 | 27.03±1.76 | 26.15±1.79 |
| D6W | 27.96±1.09 | 28.58±1.16 | 26.89±1.82 | 27.86±1.99 |
| D7W | 27.05±0.63 | 24.98±0.86 | 24.98±1.33 | 27.07±0.86 |
| D8W | 27.16±1.35 | 26.99±1.01 | 25.98±1.27 | 24.76±2.13 |
| D9W | 28.08±0.32 | 24.78±1.36 | 27.32±0.94 | 24.96±1.85 |
| D10W | 29.89±0.26 | 30.10±1.15 | 29.22±0.48 | 28.22±0.56 |
| D11W | 30.05±0.76 | 26.99±2.07 | 28.32±0.77 | 26.98±1.81 |
| D12W | 26.97±1.36 | 26.80±2.39 | 27.04±1.76 | 29.08±0.76 |
| D13W | 28.09±0.74 | 27.03±2.21 | 26.99±0.31 | 27.38±0.56 |
| D14W | 24.79±0.88 | 26.79±0.32 | 23.77±1.63 | 25.99±0.87 |
| D15W | 32.87±0.35 | 33.89±0.66 | 30.85±1.17 | 31.77±1.10 |
The influence of the 13rd week foot couple rat blood index is administered in table 7
| Inspection project | Blank | High dose | Middle dosage | Low dosage |
| WBC(109/L) | 12.02±3.98 | 10.56±2.30 | 9.89±6.76 | 12.76±4.89 |
| RBC(1012/L) | 8.01±0.36 | 7.72±0.59 | 8.10±0.35 | 7.79±0.39 |
| HGB(g/l) | 132.86±8.78 | 130.10±10.88 | 139.78±7.68 | 133.03±10.87 |
| HCT (%) | 39.88±2.36 | 41.33±3.09 | 43.78±2.35 | 41.89±2.30 |
| MCV(fl) | 50.99±1.19 | 53.99±3.35 | 53.76±1.37 | 54.03±2.39 |
| MCH(pg) | 15.88±0.36 | 17.05±0.96 | 16.89±0.30 | 16.98±0.64 |
| MCHC(g/l) | 323.96±3.75 | 319.23±4.96 | 324.04±2.02 | 320.10±7.94 |
| PLT(109/L) | 931.22±103.06 | 728.65±191.11 | 885.21±116.55 | 849.31±276.33 |
| LYMPH (%) | 738.6±4.88 | 71.07±3.76 | 85.17±4.16 | 70.79±10.66 |
The influence (%) of the 13rd week foot couple male rat organ index is administered in table 8
The influence of the 13rd week foot couple female rats blood biochemical analysis of table 9
By experimental result, the general status of administration group rat, food ration, body weight, organ weights, physiochemical indice,
Darling renal function is with control group no significant difference in normal physiologic range.Histological indications show that each internal organs of rat are equal
Unknown significance difference (p ＞ 0.05), illustrates that the Chinese medicine composition long-term taking of the present invention is non-toxic, using safety.
5th, clinical trial
(1) acne Pillsbury stagings：
1. slight is I grade, is shown as based on acne, a small amount of papule and warts, total focus number is less than 30.
2. moderate is II, III two-stage.II grade shows as acne, the papule and warts of moderate, total focus number 31-50 it
Between；III grade shows as a large amount of papules and warts, and accidental big cutaneous inflammatory lesion is widely distributed, and total focus number is between 51-100, knot
Section is less than 3.
3. severe is IV grade, shows as tubercle/cystic acne or acne conglobata, and majority has pain and forms tumour
And sinus, total focus number is more than 100, and tubercle/tumour is more than 3.
(2) the standard of curative effect evaluation
Evaluation of clinical curative effect is judged by basic healing, effective, invalid grade III Standard.
Cure：More than deflorescence >=90%, occur without new fash, no inflammation.
It is effective：More than deflorescence >=70%, occur without new fash, no inflammation.
Take a turn for the better：Fash disappears >=30% and ＜ 70%, a small amount of new breakout occurs, and inflammation mitigates.
It is invalid：Deflorescence still constantly has new fash to occur less than 30%, or without changer during treating.
Total effective rate=(curing number of cases+effective number of cases+improvement number of cases)/total number of cases × 100%
(3) clinical data
Selected 200 Genders of this research are random, and the age is between 15-45 Sui.- 4 years course of disease time half a year, extract
100 patients use Chinese medicine composition of the present invention as treatment group, and another 100 patients of extraction are control group using metronidazole, right
Than the therapeutic effect of two groups of patients.
(4) treatment method
Treated using the Chinese medicinal composition granules prepared in embodiment 5,2 times a day, once in the morning and once at night, orally,
It is 9.38g/60kgBW days that the clinical drug of the present invention, which intends dosage,.Patient's dieting is enjoined during continuous treatment medications in 4 weeks
It is pungent, hair tonic food.
(5) clinical efficacy
Specific curative effect is as shown in table 10：
The treatment group of table 10 and control group therapeutic effect compare
Statistical method has carried out data processing with SPSS19.0 statistical softwares.Data are represented with means standard deviation.Two
Compare between group and to be examined using t, rate relatively uses Chi-square Test.P ＜ 0.05 are statistically significant.
As can be seen from Table 10, treatment group P ＜ 0.05 compared with control group are statistically significant.Treatment group it is total effectively
Rate is higher than control group, illustrate to treat acne with Chinese medicine composition of the present invention evident in efficacy, worth further clinical expansion.
(6) model case
1. Nie, male, 25 years old, ill 2 years, and facial large area acne is red and swollen, shows as cystic acne, tumour and capsule
It is connected between swollen in blocks, has can't see normal skin tissue, be diagnosed as severe acne.Readme has pain, and oral external application is a variety of
Medicine is treated, in recent years recurrent exerbation, is not improved not only, and has the trend increasingly aggravated.Chinese medicine composition of the present invention is used,
Oral and external application is once in the morning and once at night；Patient is advised to avoid pungent food the thing of raw meat hair, it is red after medication one week while disable all skin care item
Swollen inflammation substantially disappears, and feels that pain itch mitigates.Two Zhou Hou faces of medication useless fellow atrophy incrustation, pain, itch disappears.With
Medicine after three weeks acne disappear completely, the colour of skin recover normal skin gloss, completely cure after, 1 year no relapse of follow-up.
2. Zhang, female 35 years old, acne is ill 3 years, geisoma nearby, cheeks, the multiple dark red papules in trigonum portion, bleb, purulence
Blister is superimposed, and quantity is more than more than 50, is diagnosed as moderate III level acne, readme used multi-medicament, recurrent exerbation.With this
After invention Chinese medicine composition, medication one week, geisoma is red and swollen nearby to be mitigated, and cheeks, the papule of trigonum, warts are reduced, after two weeks,
Geisoma is neighbouring, cheeks, the papule in trigonum portion, warts disappear, after treatment in the 3rd week, and geisoma is neighbouring, cheeks, trigonum skin
Recover normal skin tone, 1 year no relapse of follow-up.
Claims (9)
1. a kind of be used to treat the Chinese medicine composition of acne, it is characterised in that the Chinese medicine composition in parts by weight, including
Following raw material：
5-15 parts of Herba Xanthii extract, 15-30 parts of Caulis Mahoniae extract, 10-30 parts of Ajuga nippanensis extract, Calyx Seu Fructus Physalis extract
10-30 parts, 15-30 parts of gumbo flower extract, 10-30 parts of dandelion extract, 10-30 parts of Honegsukle flower P.E, Asian puccoon is extracted
5-25 parts of thing.
2. a kind of Chinese medicine composition for being used to treat acne according to claim 1, it is characterised in that the Chinese medicine composition
In parts by weight, including following raw material：
6-10 parts of Herba Xanthii extract, 20-25 parts of Caulis Mahoniae extract, 15-20 parts of Ajuga nippanensis extract, Calyx Seu Fructus Physalis extract
15-25 parts, 20-25 parts of gumbo flower extract, 15-25 parts of dandelion extract, 15-25 parts of Honegsukle flower P.E, Asian puccoon is extracted
10-15 parts of thing.
3. a kind of Chinese medicine composition for being used to treat acne according to claim 1, it is characterised in that the Chinese medicine composition
In parts by weight, including following raw material：
8 parts of Herba Xanthii extract, 22 parts of Caulis Mahoniae extract, 18 parts of Ajuga nippanensis extract, 20 parts of Calyx Seu Fructus Physalis extract, gumbo
22 parts of flower extract, 20 parts of dandelion extract, 20 parts of Honegsukle flower P.E, 12 parts of Radix Arnebiae extract.
4. a kind of preparation method for being used to treat the Chinese medicine composition of acne according to claim 1-3 any one, its
It is characterised by, this method includes：
Step one：It is prepared by Herba Xanthii extract
Siberian cocklebur grass is cleaned, crushes, is stayed overnight with the distilled water immersion of 6-8 times of crude drug amount after oven drying, in -70 DEG C of temperature 50 C
Under be heated to reflux 0.5-2.5 hours, in triplicate, merge extract solution, be concentrated under reduced pressure, add ethanol and stir, stand, will be miscellaneous
Matter is filtered, and supernatant is concentrated under reduced pressure to obtain Herba Xanthii extract；
Step 2：It is prepared by Caulis Mahoniae extract
Leatherleaf mahonia is cleaned, crushed after oven drying, with the distilled water refluxing extraction of 6-8 times of crude drug amount, in -70 DEG C of temperature 50 C
Lower heating extraction 0.5-2.5 hours, in triplicate, merges extract solution, is concentrated under reduced pressure, D-101 types macroporous resin column on concentrate,
The removal of impurity is gone, with elution, eluent is collected, is concentrated under reduced pressure to give Caulis Mahoniae extract；
Step 3：It is prepared by Ajuga nippanensis extract
Japanese bugle herb is extracted using 70% ethanol, soaked overnight, heating water bath, refluxing extraction three times, described Extracting temperature
For 50 DEG C -80 DEG C, the single-trial extraction time is 0.5-2.5 hours, merges extract solution, is concentrated, filtering, and D-101 types are big on concentrate
Hole resin column, goes the removal of impurity, then with volume fraction is respectively 10%, 30%, 50% ethanol gradient elution, takes 50% elution portion
Decompression is divided to obtain Ajuga nippanensis extract.
Step 4：It is prepared by Calyx Seu Fructus Physalis extract
Wintercherry fruit or calyx is cleaned, crushed after being dried, using 85% ethanol, solid-liquid ratio 1:5-1:8, heating water bath, refluxing extraction three times,
Extracting temperature is 50 DEG C -80 DEG C, each 0.5-2.5 hours, merges extract solution, is concentrated, filtering, then through D-101-I type macropore trees
Fat is adsorbed, and is collected 50% and is eluted the position oven drying that is concentrated under reduced pressure and produces Calyx Seu Fructus Physalis extract；
Step 5：It is prepared by gumbo flower extract
Okra flowers are cleaned, crushed after being dried, using 85% ethanol, solid-liquid ratio 1:5-1:8, heating water bath, refluxing extraction three times,
Extracting temperature is 70 DEG C -80 DEG C, each 0.5-2.5 hours, merges extract solution, is concentrated, filtering, then through D-108 type macroreticular resins
Absorption, collects 60% and elutes the position oven drying that is concentrated under reduced pressure and produce gumbo flower extract；
Step 6：Mixing
By Herba Xanthii extract, Caulis Mahoniae extract, Ajuga nippanensis extract, Calyx Seu Fructus Physalis extract, gumbo flower extract, Pu Gong
English extract, Honegsukle flower P.E and Radix Arnebiae extract mixing, cross 60-80 mesh sieves, produce after dry, pulverize at 70 DEG C -85 DEG C
To the Chinese medicine composition for treating acne.
5. a kind of preparation method for being used to treat the Chinese medicine composition of acne according to claim 4, it is characterised in that institute
The ethanol mass fraction of the step of stating one is 60-80%.
6. a kind of preparation method for being used to treat the Chinese medicine composition of acne according to claim 4, it is characterised in that institute
The time of repose of the step of stating one is 1-3h.
7. a kind of preparation method for being used to treat the Chinese medicine composition of acne according to claim 4, it is characterised in that institute
The eluent of the step of stating two is hydrochloric acid solution.
8. a kind of preparation method for being used to treat the Chinese medicine composition of acne according to claim 4, it is characterised in that institute
The concentration of the hydrochloric acid solution of the step of stating two is 0.04-0.08mol/L.
9. a kind of preparation method for being used to treat the Chinese medicine composition of acne according to claim 4, it is characterised in that institute
The japanese bugle herb and the mass ratio of ethanol stated are 1:3-1:8.
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