CN103933209B - A kind of method extracted in the stem from wintercherry fruit or calyx and detect steroid saponin - Google Patents
A kind of method extracted in the stem from wintercherry fruit or calyx and detect steroid saponin Download PDFInfo
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- CN103933209B CN103933209B CN201410131662.2A CN201410131662A CN103933209B CN 103933209 B CN103933209 B CN 103933209B CN 201410131662 A CN201410131662 A CN 201410131662A CN 103933209 B CN103933209 B CN 103933209B
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Abstract
A kind of method extracted in stem from wintercherry fruit or calyx and detect steroid saponin, is related to medicine extracting method, step：After dry wintercherry fruit or calyx stem is crushed, with 380~680W microwave irradiation powers, concentration of alcohol is 60%~90%, is extracted 1~5 time, extracts 5min~15min, solid-liquid ratio 1:10~1:40；After extraction, filtering, merge extract solution concentration and medicinal extract is made, after medicinal extract is dissolved, measured by the perchloric acid colorimetric method of standard items vanillic aldehyde one of Dioscin, saponin content in extract is calculated, the microwave loss mechanisms can substantially shorten extraction time, improve steroid saponin yield, and can make full use of the medicine-food two-purpose resource of steroid saponin in wintercherry fruit or calyx stem.Steroid saponin in the wintercherry fruit or calyx stem extracted by the method can be used as the medicine for preparing antibacterial, eliminating the phlegm, platelet aggregation-against, reducing blood lipid, antitumor etc. after isolating and purifying or be used as the raw material of functional food, health products.
Description
Technical field
The present invention relates to one kind to be related to medicine extracting method, extracts and detect steroid from wintercherry fruit or calyx stem more particularly to one kind
The method of body saponin(e.
Background technology
Wintercherry fruit or calyx（physalis alkekengi）, alias wintercherry, hang up a Chinese lantern, red Miss etc., be Solanaceae monkey flower,
Fruit is that spheroidal berry is perennial herb, and its fruit is edible, is the time-honored medicine-food two-purpose health care in China
Type fruit.Also recorded in its history tree.Main product is in the Three Eastern Provinces, Hebei, Xinjiang, Shandong and other places.The composition of wintercherry fruit or calyx is main
There are steroid, sterols, alkaloids, amino acids, volatile oil, polysaccharide, inorganic elements etc..Pharmacological research shows wintercherry fruit or calyx
With the good pharmacological action such as anti-inflammatory, antimalarial, anticancer, antibacterial.According to the basic structure of sapogenin, saponin(e is divided into steroid saponin
And triterpenoid saponin.The saponin(e contained in wintercherry fruit or calyx is mainly steroid saponin, and research shows that Dioscin is different spirostan alcohol type steroid
Body saponin(e, it has the effect such as eliminating the phlegm, platelet aggregation-against, reducing blood lipid, antitumor.According to another document report, in franchet groundcherry calyx or fruit
Steroid saponin similar with diabetes pill all there is blood sugar reducing function.And the triterpene soap similar with the steroid saponin structure in wintercherry fruit or calyx
Glycosides, as ginsenoside Rg1 can protect dopamine neuron, can reduce lipid content treatment of vascular can also prevent that cerebral artery is athero-
Hardening, the effect such as expression of blood vessel endothelium matrix metalloproteinase can be suppressed.
The process that the principle of Microwave Extraction is mainly microwave radiation heat transfer is that frequency electromagnetic waves reach extract through medium
In internal cell, so as to which the electromagnetic field of microwave can accelerate the molecular diffusion rate of extract components.It can be determined by microwave principle
The inventive method can improve extraction efficiency, and save the time.The extraction of conventional wintercherry fruit or calyx steroid saponin mainly uses water-bath
Effect of Alcohol Extracting Procedureto Total, during this method operating cost, cumbersome, product yield is low, and energy consumption is big.
The content of the invention
It is an object of the invention to provide a kind of method extracted in stem from wintercherry fruit or calyx and detect steroid saponin, this method will
After dry wintercherry fruit or calyx stem and place calyx crush, feed liquid is extracted, medicinal extract is made, develops the medicinal and edible resource of wintercherry fruit or calyx stem, together
When, Microwave Extraction technique can significantly reduce extraction time, improve the yield of saponin(e.The wintercherry fruit or calyx stem obtained through the inventive method
Steroid saponin with place calyx can be as preparation antibacterial, eliminating the phlegm, platelet aggregation-against, reducing blood lipid, antitumor etc. through isolating and purifying
Medicine or raw material as functional food, health products use.
The purpose of the present invention is achieved through the following technical solutions：
A kind of method extracted in stem from wintercherry fruit or calyx and detect steroid saponin, methods described include following process：
A. sample treatment：
Wintercherry fruit or calyx stem is dried in 100~105 DEG C of baking ovens, be ground into pulverizer it is powdered, cross 40~80 mesh sieves postpone
It is stand-by in being sealed up for safekeeping in dry valve bag；
B. multistage microwave amplifier：
1~5g stems accurately are weighed, measure concentration of alcohol for 60%~90% in 250ml round-bottomed flasks, according to set experiment
Scheme, extract 1~5 time, extract 5min~15min, solid-liquid ratio 1:10~1:40；In microwave irradiation power 380~680W conditions
Lower carry out Microwave Extraction；After extraction, filter, concentrated by rotary evaporation, remove and medicinal extract is made, it is stand-by；
C. the foundation of standard curve：
Dioscin standard items are weighed in volumetric flask, titer is made into absolute methanol constant volume, take titer to add fragrant
Oxalaldehyde-laggard traveling wave the long scan of perchloric acid colour developing, measures maximum wavelength；The titer for pipetting quantitative gradient dilution respectively is filled in tool
In test tube, after water-bath volatilizes, 0.1~0.5ml is separately added into, 2~5% vanillic aldehydes-glacial acetic acid liquid, 0.5~1.0ml perchloric acid,
65~70 DEG C of water-baths, heat 10~15min, frozen water cooling；Add 4.0~6.0ml glacial acetic acid and stand 10~30min, in maximum
Absorbance is determined at wavelength, with absorbance (A) for ordinate Y, the concentration C of Dioscin does standard curve for abscissa X, and builds
Vertical regression equation；
D. the measure of the content of steroid saponin：
Using the content of vanillin-perchloric acid measurement saponin(e：Extract after revolving is settled in volumetric flask；Pipette
Quantitative above-mentioned solution is placed in tool plug test tube, and water-bath volatilizes solvent；Then plus 0.1~0.5ml, 2~5% vanillic aldehyde-ice
Acetum and 0.5~1.0ml perchloric acid, close plug test tube shake up, and are placed in 10 ~ 15min of vibration heating in 65~70 DEG C of water-baths；Take
Cooled down immediately with frozen water after going out；Add 4.0~6.0ml glacial acetic acid to mix, stand 10~30min.Surveyed in maximum absorption wave strong point
Measure absorbance；The absorbance obtained according to measurement substitutes into the regression equation calculation saponin(e yield of standard curve；
Above-mentioned percentage is percent by volume.
Embodiment
Illustrated embodiment below in conjunction with the accompanying drawings, the invention will be further described.
Embodiment 1：
(1) wintercherry fruit or calyx stem is dried in 100~105 DEG C of baking ovens, be ground into pulverizer it is powdered, cross 40~80 mesh sieves put
It is stand-by after being sealed up for safekeeping in dry valve bag；
(2) the foundation of standard curve：5~10mg Dioscins are weighed to be made into absolute methanol constant volume in 50ml volumetric flasks
0.05~0.2mg/ml titer, takes titer to add the laggard traveling wave long scan of vanilla root rot colour developing, and maximum wavelength is
500~560nm.Pipette 300 respectively, 400,500,1100,1300,1500ul in tool plug test tube, after water-bath volatilizes, respectively plus
Enter 0.1~0.5ml（2~5%）Vanillic aldehyde-glacial acetic acid liquid, 0.5~1.0ml perchloric acid, 65~70 DEG C of water-baths, heating 10~
15min, frozen water cooling；Add 4.0~6.0ml glacial acetic acid and stand 10~30min, absorbance is determined at 560nm.With absorbance
(A) it is ordinate Y, the concentration C of Dioscin does standard curve for abscissa X, and establishes regression equation；
(3) accurately weigh three parts of 1~5g wintercherry fruit or calyx stem powder, respectively with 60~90% ethanol solutions, extraction 5~15min,
Extract 1~5 time, solid-liquid ratio 1:10~1:40, extracted under the conditions of 380~680W of radiant power；
(4) extraction finishes, and filters, concentrated by rotary evaporation, removes, and medicinal extract is made, stand-by；
(5) contents of steroid saponin is determined with vanillin-perchloric acid, the extract after revolving is settled to 100ml capacity
In bottle；Pipette 0.1ml to be placed in 10ml tool plug test tubes, 60 DEG C of water-baths volatilize solvent；Then 0.1~0.5ml is added（2~
5%）Vanillic aldehyde-glacial acetic acid liquid, 0.5~1.0ml perchloric acid, 65~70 DEG C of water-baths, heat 10~15min, frozen water cooling；Add
4.0~6.0ml glacial acetic acid stands 10~30min, and absorbance is measured in maximum absorption wave strong point.The extinction obtained according to measurement
Angle value substitutes into the regression equation of standard curve.It is more than 5.0% by the steroid saponin yield for calculating stem.
Embodiment 2：
(1) franchet groundcherry calyx or fruit is dried in 100~105 DEG C of baking ovens, and powdered, 40~80 mesh sieves excessively are ground into pulverizer
Postpone stand-by in being sealed up for safekeeping in dry valve bag；
(2) the foundation of standard curve：With it is (2) identical in embodiment 1.
(3) accurately weigh three parts of 0.1~0.5g franchet groundcherry calyx or fruit powder, respectively with 60~90% ethanol solutions, extraction 5~
15min, extraction 1~5 time, solid-liquid ratio 1:10~1:40, extracted under the conditions of 380~680W of radiant power；
(4) extraction finishes, and filters, concentrated by rotary evaporation, removes, and medicinal extract is made, stand-by；
(5) contents of steroid saponin is determined with vanillin-perchloric acid, the extract after revolving is settled to 100ml capacity
In bottle；Pipette 0.1ml to be placed in 10ml tool plug test tubes, 60 DEG C of water-baths volatilize solvent；Then 0.1~0.5ml is added（2~
5%）Vanillic aldehyde-glacial acetic acid liquid, 0.5~1.0ml perchloric acid, 65~70 DEG C of water-baths, heat 10~15min, frozen water cooling；Add
4.0~6.0ml glacial acetic acid stands 10~30min, and absorbance is measured in maximum absorption wave strong point.The extinction obtained according to measurement
Angle value substitutes into the regression equation of standard curve.It is more than 8.0% by calculating steroid saponin yield in the calyx of place.
Claims (1)
1. a kind of method extracted in stem from wintercherry fruit or calyx and detect steroid saponin, it is characterised in that methods described includes following mistake
Journey：
A. sample treatment：
Wintercherry fruit or calyx stem is dried in 100~105 DEG C of baking ovens, be ground into pulverizer it is powdered, cross 40~80 mesh sieves after be placed in it is dry
Sealed up for safekeeping in dry valve bag stand-by；
B. multistage microwave amplifier：
1 ~ 5g stems accurately are weighed, measure concentration of alcohol for 60%~90% in 250ml round-bottomed flasks, extraction 1~5 time, extraction
5min~15min, solid-liquid ratio 1:10~1:40；Microwave Extraction is carried out under the conditions of 380~680W of microwave irradiation power；Extract
Bi Hou, filter, concentrated by rotary evaporation, remove and medicinal extract is made, it is stand-by；
C. the foundation of standard curve：
Dioscin standard items are weighed in volumetric flask, titer is made into absolute methanol constant volume, take titer add vanillic aldehyde-
The laggard traveling wave long scan of perchloric acid colour developing, measures maximum wavelength；The titer of quantitative gradient dilution is pipetted respectively in tool plug test tube
In, after water-bath volatilizes, it is separately added into 0.1~0.5ml, 2~5% vanillic aldehydes-glacial acetic acid liquid, 0.5~1.0ml perchloric acid, 65~
70 DEG C of water-baths, heat 10~15min, frozen water cooling；Add 4.0~6.0ml glacial acetic acid and stand 10~30min, in maximum wavelength
Place's measure absorbance, with absorbance (A) for ordinate Y, the concentration C of Dioscin does standard curve for abscissa X, and establishes back
Return equation；
D. the measure of the content of steroid saponin：
Using the content of vanillin-perchloric acid measurement saponin(e：Extract after revolving is settled in volumetric flask；Pipette quantitative
Above-mentioned solution is placed in tool plug test tube, and water-bath volatilizes solvent；Then plus 0.1~0.5ml, 2~5% vanillic aldehyde-glacial acetic acid
Solution and 0.5~1.0ml perchloric acid, close plug test tube shake up, and are placed in 10 ~ 15min of vibration heating in 65~70 DEG C of water-baths；After taking-up
Cooled down immediately with frozen water；Add 4.0~6.0ml glacial acetic acid to mix, stand 10~30min；Measure and inhale in maximum absorption wave strong point
Shading value；The absorbance obtained according to measurement substitutes into the regression equation calculation saponin(e yield of standard curve；Above-mentioned percentage is equal
For percent by volume.
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| CN105816569A (en) * | 2015-01-08 | 2016-08-03 | 辽宁医联新药研究所 | Method for extracting calyx seu fructus physalis anti-pharyngitis effective part and its total steroid as well as refining method thereof |
| CN108148107A (en) * | 2017-12-25 | 2018-06-12 | 华远医药研究院有限公司 | The method for extraction and purification of Dioscin |
| CN113583470B (en) * | 2021-07-31 | 2023-05-23 | 彼悦(北京)科技有限公司 | Multifunctional composite vegetable dye and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1742845A (en) * | 2005-09-28 | 2006-03-08 | 大连美罗中药厂有限公司 | Medicine for treating rheumatism and rheumatoid diseases and preparing method |
| CN1864717A (en) * | 2006-06-14 | 2006-11-22 | 哈尔滨医科大学 | Method for extracting Chinese lantern plant steride and pharmaceutical composition thereof |
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| CN2864717Y (en) * | 2005-12-08 | 2007-01-31 | 陈广生 | Anti-tension type jig for optical fiber composite phase wire |
| CN101244153B (en) * | 2008-02-01 | 2012-05-09 | 张炯怡 | Application of alkekengi in preparing medicament for treating digestive system disease |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1742845A (en) * | 2005-09-28 | 2006-03-08 | 大连美罗中药厂有限公司 | Medicine for treating rheumatism and rheumatoid diseases and preparing method |
| CN1864717A (en) * | 2006-06-14 | 2006-11-22 | 哈尔滨医科大学 | Method for extracting Chinese lantern plant steride and pharmaceutical composition thereof |
Non-Patent Citations (2)
| Title |
|---|
| 中药材皂苷提取方法与工艺研究;郭晓宇，等。;《内蒙古医科大学学报》;20130215;第35卷(第S1期);第118页"10微波提取法" * |
| 锦灯笼宿萼皂苷降血糖作用研究;刘雅丽，等;《东北师大学报（自然科学版）》;20100620;第42卷(第2期);第106页"1.2.1"第1段 * |
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Inventor after: Gao Pinyi Inventor after: Liu Xuegui Inventor after: Gao Yixing Inventor after: Wang Le Inventor after: Zhang Zhenxue Inventor before: He Haitao Inventor before: Sun Yanyan Inventor before: Wan Yanping Inventor before: Jiang Fuyu Inventor before: Liu Xuegui Inventor before: Gao Pinyi |
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