CN103734191B - A kind of zymotic fluid and application thereof with nematoda control effect - Google Patents
A kind of zymotic fluid and application thereof with nematoda control effect Download PDFInfo
- Publication number
- CN103734191B CN103734191B CN201310733431.4A CN201310733431A CN103734191B CN 103734191 B CN103734191 B CN 103734191B CN 201310733431 A CN201310733431 A CN 201310733431A CN 103734191 B CN103734191 B CN 103734191B
- Authority
- CN
- China
- Prior art keywords
- zymotic fluid
- chicken manure
- waste liquid
- cassava
- alcohol waste
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The present invention relates to agriculture organic pharmaceutical arts, specifically provide a kind of zymotic fluid with nematoda control effect, described zymotic fluid is mixed into fermentation substrate by fresh chicken manure and molasses alcohol waste liquid, becomes thoroughly decomposed and becomes zymotic fluid after adding clear water at 20 DEG C ~ 35 DEG C condition bottom fermentations.Described zymotic fluid has Meloidogyne incognita well to be killed and inhibitory action, can be used as the typical nematode inhibitor that non-chemically synthesizes and uses for a long time in field ecosystem, be particularly useful for organic farming.
Description
Technical field
The present invention relates to agriculture organic pharmaceutical arts, specifically, relate to a kind of zymotic fluid and the application thereof with nematoda control effect.
Background technology
The economic loss that root-knot nematode is caused world agriculture is every year up to 1,570 hundred million.Non-chemically synthesize the favor that the nematode poisoning agent of class and nematode inhibitor are more and more subject to people.Abundant at rainfall, the tropical and subtropical zone area had a moderate climate, the harm of root-knot nematode is particularly serious.In China, such area is also important cassava producing region and livestock and poultry cultivation area simultaneously.Up to now, cassava molasses alcohol waste liquid and freshly-slaughtered poultry excrement about by the report of chicken manure stack retting with the liquid manure fertilizing soil that becomes thoroughly decomposed, but are made into the report that fermenting substrate provides the zymotic fluid of nematode preventive and therapeutic effect and still locate blank by existing part.
The nematode inhibitor non-chemically synthesized more and more receives the concern of people, but loaded down with trivial details manufacturing process and high cost make this work idea difficulty of implementation very large.The existing report about use large-scale installation for fermenting, chicken manure being made organic fertilizer liquid at present, but simple chicken manure fermenting liquid does not have and effectively kills line, presses down line effect under low consistency conditions, and there is no the method effectively can using high concentration chicken manure fermenting liquid under fertigation condition.And the residue in high gravity fermentation liquid understands the filter of Severe blockage dropper system, and the 3-4% that fertile pump discharge can only account at most main pipeline discharge is noted in common large-scale irrigated area of taking turns.
The present invention is intended to cassava molasses alcohol waste liquid and freshly-slaughtered poultry excrement to be made into fermentation substrate and is prepared into the zymotic fluid having and kill line, press down line effect, fills up the blank of prior art.Meanwhile, the reasonable disposal of freshly-slaughtered poultry excrement and cassava molasses alcohol waste liquid, is related to rural environment Quality advance and agricultural sustainable development, has positive double meaning.
Summary of the invention
In order to solve problems of the prior art, the object of this invention is to provide a kind of have nematoda control effect zymotic fluid and application.
In order to realize the object of the invention, of the present inventionly first provide a kind of zymotic fluid with nematoda control effect, described zymotic fluid is mixed into fermentation substrate by fresh chicken manure and molasses alcohol waste liquid, and becoming thoroughly decomposed more than 45 days at 20 DEG C ~ 35 DEG C condition bottom fermentations after adding clear water becomes zymotic fluid.
Further, described fresh chicken manure and molasses alcohol waste liquid are mixed into fermentation substrate according to the ratio of 5:3.
Further, after adding clear water, need ensure that the concentration of fermentation substrate is 40-45%.
As preferably, described fresh chicken manure to be water content be 94% ~ 97% fresh chicken manure.
As preferably, described molasses alcohol waste liquid to be water content be 90% ~ 95% molasses alcohol waste liquid.
As preferably, described molasses alcohol waste liquid is selected from cassava molasses alcohol waste liquid.
Present invention also offers the zymotechnique of aforesaid fermentation liquid: fresh chicken manure and molasses alcohol waste liquid are mixed into fermentation substrate in proportion, add injected clear water after fermentation vat, after this consume according to evaporation and supplement clear water to maintain concentration of substrate in pond, when zymotic fluid is that black is thick, homogeneous, during without bulky grain solid content without pungent foul odour, fermentation maturity terminates.
As preferably, fresh chicken manure and molasses alcohol waste liquid layering can be added in fermentation vat.
Invention further provides aforesaid fermentation liquid in poisoning root-knot nematode and the application in suppressing root-knot nematode pieces of an egg to be hatched.
Beneficial effect of the present invention is:
The zymotechnique of zymotic fluid of the present invention is simple to operation, and substrate source is extensively with low cost, and fermentation process is without the need to adding any other chemical agent.Described zymotic fluid has Meloidogyne incognita well to be killed and inhibitory action, can be used as the typical nematode inhibitor that non-chemically synthesizes and uses for a long time in field ecosystem, be particularly useful for organic farming.The reasonable disposal of freshly-slaughtered poultry excrement and cassava molasses alcohol waste liquid has double meaning to the improvement of rural environment quality and agricultural sustainable development.
Accompanying drawing explanation
Fig. 1 is the device for carrying out said structure of zymotic fluid zymotechnique of the present invention.
Fig. 2 be in the embodiment of the present invention 2 different solutions process to the lethal effect of root-knot nematode second instar larvae.
Fig. 3 is that in the embodiment of the present invention 3, different solutions process hatches inhibition to the pieces of an egg of root-knot nematode.
Embodiment
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
The fermentation of embodiment 1 zymotic fluid
Fig. 1 shows the device for carrying out said structure of zymotic fluid zymotechnique of the present invention, and wherein three low-level culture pools of the top are the reaction tanks for fermentation substrate, and the secondary pond of below, three grades of ponds are only for filtering the filtrate of foreign material and mixing three low-level culture pools.Priming apparatus is only located in 1 grade of pond, and two, three grades of ponds no longer additionally add water.After three-stage filtration, filtrate applies field by water-fertilizer integral stem system with drip irrigation.
Fermentation process mainly carries out in 1 grade of pond, and pond body is total measurement (volume) 1000m
3cube, material is concrete, long 16.0m, wide 12.5m, dark 5.0m.Body top, pond is provided with water filling device, and water source is river.Be provided with sunshade above 1 grade of pond, reduce evaporation discharge and rainwater perfusion.
First fill the fresh chicken manure of water content between 94% ~ 97% (being after this called for short rare chicken manure) 150 tons, calculate with 50 tons of charging efficiencies every day and need fill 3 days.
Secondly fill the cassava molasses alcohol waste liquid (after this referred to as molasses alcohol waste liquid) 100 ton of water content between 90% ~ 95%, calculate with 50 tons of charging efficiencies every day and need fill 2 days.
3rd step fills rare chicken manure 100 tons, and calculating with 50 tons of charging efficiencies every day needs 2 days.
4th step fills molasses alcohol waste liquid 50 tons, and calculating with 50 tons of charging efficiencies every day needs 1 day.
Substrate is filled total amount accumulation and is reached 400 tons of front and back beginnings to water filling in fermentation vat, makes zymotic fluid cumulative volume in pond reach 900m
3stop time upper and lower pouring water, after this every two weeks supplements a certain amount of clear water to make up the loss of evaporating and causing in pond, need ensure that the concentration of fermentation substrate is 40-45%.
This zymotechnique needs could carry out smoothly when temperature and pool inner water temperature reach 20 ~ 35 DEG C, for Guangxi province, fermentation work be suitable for May ~ September carries out.
Along with becoming thoroughly decomposed gradually of zymotic fluid, the foul smell of chicken manure can change mellow stink into gradually, and fermentation can produce a large amount of bubble in pond, peak, belongs to normal phenomenon.Zymotic fluid after becoming thoroughly decomposed is that black is thick, homogeneous, without bulky grain solid content without pungent foul odour.
Embodiment 2 zymotic fluid is on the impact of root-knot nematode second instar larvae lethality rate
Adopt zymotic fluid prepared by embodiment 1 zymotechnique, and the pure chicken manure fermenting liquid of preparation (is replaced by except equivalent live chickens excrement except by molasses waste liquid in embodiment 1 in addition, other are constant, after this be called for short chicken manure water), by compounding to zymotic fluid and equal proportion after chicken manure water filtration become mixed culture fermentation liquid (being after this called for short mixed liquor).According to the dilution characteristic of drip irrigation system to stoste, setting clear water, chicken manure water, 10% chicken manure water, 4% chicken manure water, 2% chicken manure water; Mixed liquor, 10% mixed liquor, 4% mixed liquor, 2% mixed liquor; Zymotic fluid, 10% zymotic fluid, 4% zymotic fluid, 13 the different solutions process of 2% zymotic fluid are tested, and each process arranges 3 repetitions.
The pumpkin plot serious from eelworm harm obtains nematode sample, and pumpkin root system is clean with tap water, and the root-knot nematode pieces of an egg fastened by cushaw root are subsequently chosen down with for subsequent use.Pieces of an egg under choosing are positioned over above the sieve being covered with layers of absorbent paper, sieve are put into culture dish, adds appropriate clear water, make the water surface just in time can not have pieces of an egg.Under culture dish is positioned over room temperature, collect the solution in culture dish after 24h, because the second instar larvae of root-knot nematode has hydrotropism, on sieve, the second instar larvae of hatching can pass blotting paper, in the aqueous solution below culture dish.Therefore a large amount of root-knot nematode second instar larvae is contained in the aqueous solution.
Being dripped by the root-knot nematode second instar larvae suspension collected partly drips in 24 porocyte culture plates, and drip the variable concentrations chicken manure aqueous solution respectively subsequently, build lid, room temperature lucifuge is placed.Observed and recorded verge of death borer population after 24h, calculates lethality.Computing formula is as follows:
To the lethal effect of second instar larvae as shown in Figure 1, wherein zymotic fluid can reach and kill line effect (67.36%) preferably 13 kinds of solution under 2% concentration, and killing line rate under 4% concentration can reach 78.67%, and 10% concentration is issued to best effects 87.80%.Conventional chicken manure fermenting liquid compared by the zymotic fluid using this technique to ferment better nematode killing effect.
Embodiment 3 zymotic fluid is on the impact of root-knot nematode pieces of an egg hatching relative inhibition
Adopt zymotic fluid prepared by embodiment 1 zymotechnique, and the pure chicken manure fermenting liquid of preparation (is replaced by except equivalent live chickens excrement except by molasses waste liquid in embodiment 1 in addition, other are constant, after this be called for short chicken manure water), by compounding to zymotic fluid and equal proportion after chicken manure water filtration become mixed culture fermentation liquid (being after this called for short mixed liquor).According to the dilution characteristic of drip irrigation system to stoste, setting chicken manure water, 10% chicken manure water, 4% chicken manure water, 2% chicken manure water; Mixed liquor, 10% mixed liquor, 4% mixed liquor, 2% mixed liquor; Zymotic fluid, 10% zymotic fluid, 4% zymotic fluid, 12 the different solutions process of 2% zymotic fluid are tested, and each process arranges 3 repetitions.
The pumpkin plot serious from eelworm harm obtains nematode sample, by clean for pumpkin root system tap water, picking cushaw root is fastened uniform pieces of an egg and is positioned in 2ml centrifuge tube subsequently, 3 pieces of an egg and 1ml solution to be measured is placed in each centrifuge tube, with clear water in contrast, the nematode number of hatching in pipe is measured, continuously record 5 days every 24h.Computing formula is as follows:
The relative inhibition that 12 kinds of solution is hatched nematode pieces of an egg as shown in Figure 2, wherein zymotic fluid under 2% concentration to the hatching relative inhibition of nematode pieces of an egg up to 98%, the increase zymotic fluid with concentration remains extraordinary nematode pieces of an egg hatching inhibiting rate.The zymotic fluid using this technique to ferment is compared the pieces of an egg that conventional chicken manure fermenting liquid has a highly significant and is suppressed advantage.From interpretation of result, the zymotic fluid using this zymotechnique to produce is grouped into relative to the one-tenth that jungle fowl manure fermentation liquid works and necessarily there occurs change.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (7)
1. have a zymotic fluid for nematoda control effect, it is characterized in that, described zymotic fluid is mixed into fermentation substrate by fresh chicken manure and cassava molasses alcohol waste liquid, and becoming thoroughly decomposed more than 45 days at 20 DEG C ~ 35 DEG C condition bottom fermentations after adding clear water becomes zymotic fluid; After adding clear water, need ensure that the concentration of fermentation substrate is 40-45%;
Described fresh chicken manure and cassava molasses alcohol waste liquid are mixed into fermentation substrate according to the ratio of 5:3.
2. zymotic fluid according to claim 1, is characterized in that, described fresh chicken manure to be water content be 94% ~ 97% fresh chicken manure.
3. zymotic fluid according to claim 1 and 2, is characterized in that, described cassava molasses alcohol waste liquid to be water content be 90% ~ 95% cassava molasses alcohol waste liquid.
4. the zymotechnique of the zymotic fluid described in claim 1-3 any one, it is characterized in that, fresh chicken manure and cassava molasses alcohol waste liquid are mixed into fermentation substrate in proportion, add injected clear water after fermentation vat, after this consume according to evaporation and supplement clear water to maintain concentration of substrate in pond, when zymotic fluid is that black is thick, homogeneous, during without bulky grain solid content without pungent foul odour, fermentation maturity terminates.
5. zymotechnique according to claim 4, is characterized in that, fresh chicken manure and the layering of cassava molasses alcohol waste liquid is added in fermentation vat.
6. the application of the zymotic fluid described in claim 1-3 any one in poisoning root-knot nematode.
7. the zymotic fluid described in claim 1-3 any one is suppressing the application in the hatching of root-knot nematode pieces of an egg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310733431.4A CN103734191B (en) | 2013-12-26 | 2013-12-26 | A kind of zymotic fluid and application thereof with nematoda control effect |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310733431.4A CN103734191B (en) | 2013-12-26 | 2013-12-26 | A kind of zymotic fluid and application thereof with nematoda control effect |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103734191A CN103734191A (en) | 2014-04-23 |
| CN103734191B true CN103734191B (en) | 2015-09-30 |
Family
ID=50491328
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310733431.4A Active CN103734191B (en) | 2013-12-26 | 2013-12-26 | A kind of zymotic fluid and application thereof with nematoda control effect |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103734191B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105294695B (en) * | 2015-09-17 | 2017-11-07 | 中国农业大学 | A kind of compound for being isolated from farges evodia fruit and its pest-resistant purposes |
| CN105646011A (en) * | 2016-01-11 | 2016-06-08 | 济南康众医药科技开发有限公司 | New application of trogopterus dung |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1546437A (en) * | 2003-12-01 | 2004-11-17 | 华中农业大学 | Organic fertilizer composed of waste liquid and waste residue and decomposing leaven from sugar refinery and method for preparing the same |
| CN103058723A (en) * | 2011-10-18 | 2013-04-24 | 姚昂 | Preparation method of chicken manure organic fertilizer |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04327505A (en) * | 1991-04-23 | 1992-11-17 | Riyokuken Ekorojii Kenkyusho:Kk | Control of disease and insect damage |
-
2013
- 2013-12-26 CN CN201310733431.4A patent/CN103734191B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1546437A (en) * | 2003-12-01 | 2004-11-17 | 华中农业大学 | Organic fertilizer composed of waste liquid and waste residue and decomposing leaven from sugar refinery and method for preparing the same |
| CN103058723A (en) * | 2011-10-18 | 2013-04-24 | 姚昂 | Preparation method of chicken manure organic fertilizer |
Non-Patent Citations (2)
| Title |
|---|
| 农业废弃物堆肥对根结线虫的抑制作用;王博;《环境科学动态》;20050131(第1期);第12-14页 * |
| 蔬菜根结线虫病无公害防治技术研究的新进展;李英梅 等;《中国农学通报》;20080731;第24卷(第7 期);第370页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103734191A (en) | 2014-04-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102219332B (en) | Novel technology for treating domestic sewage in rural area | |
| CN102845203B (en) | Method for planting alfalfa in moderately severe alkaline sulfuric acid saline soil on large scale | |
| CN105272472A (en) | Biogas slurry formula fertilizer through fermentation of primary pig manure and urine and preparation method thereof | |
| CN102874985B (en) | Method for treating waste pollutants in pig farm | |
| CN101869087A (en) | Environmentally-friendly pond culture method for freshwater shrimps | |
| CN104663377A (en) | Soilless culture method for chives | |
| CN105724287A (en) | Ecological environmental protection circulating planting and breeding method | |
| CN103734191B (en) | A kind of zymotic fluid and application thereof with nematoda control effect | |
| CN202014519U (en) | Structure of ecological slope protection purifying culture water body in pond | |
| CN103396176A (en) | Production process for converting poultry excrement into commercial organic fertilizer | |
| CN204281416U (en) | A kind of artificial wet land system for marine culture wastewater process | |
| CN104957063B (en) | River crab master supports the ecological prevention method of pond moss | |
| CN104386896B (en) | A kind of aquaculture wastewater purifying treatment method | |
| CN105230202B (en) | The implantation methods of sugarcane | |
| CN106365907A (en) | Organic fertilizer with sweet potato residues as raw material | |
| CN112042350B (en) | Method for applying farm manure water to drip irrigation system | |
| CN100556829C (en) | Utilize deep-flow technique to repair the method for eutrophication water | |
| CN106518320A (en) | A preparing method of a cultivation medium for Xisha watermelon | |
| CN101580300A (en) | Method for purifying culturing water in pond ecological protection slope | |
| Anh et al. | Effect of management practices and fertilization with biodigester effluent on biomass yield and composition of duckweed | |
| CN105028494A (en) | Chromolaena odorata-source water hyacinth growth regulating agent and preparation method | |
| CN110463723A (en) | It is a kind of for the solid-state microorganism herbicide of paddy field, preparation method and applications | |
| Warade et al. | Review of biogas production from various substrates and co-substrates through different anaerobic reactor | |
| CN108432384A (en) | The method for reducing salt-soda soil pH using alkaline pig farm biogas slurry | |
| CN211946449U (en) | Device of residential quarter domestic sewage cyclic utilization zero release |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |