CN103733996A - Cultivation method of sugarcane seedlings - Google Patents
Cultivation method of sugarcane seedlings Download PDFInfo
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- CN103733996A CN103733996A CN201310717159.0A CN201310717159A CN103733996A CN 103733996 A CN103733996 A CN 103733996A CN 201310717159 A CN201310717159 A CN 201310717159A CN 103733996 A CN103733996 A CN 103733996A
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- sugarcane
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Abstract
The invention discloses a cultivation method of sugarcane seedlings. In the method, the stem tips are immersed into hot water for treatment and then subjected to in-vitro cultivation. The cultivation method of sugarcane seedlings disclosed by the invention is a healthy seedling production technology widely adopted internationally; the cultivation management mode is not changed too much and is easily acceptable to the sugarcane farmers; the growth advantage of the sugarcane seedlings is obvious, the sugarcane seedlings grow early and develop quickly with early jointing and healthy and strong appearance, and the conditions in the whole growth period are better than those of common seedlings; the technical indexes of the detoxified seedlings are higher than those of other seedlings, for example, the parameter indexes such as photosynthetic rate of blade, height of sugarcane seedling plant, diameter of sugarcane stem and productive tiller number; and the yield and sugar content are obviously increased, and the yield of sugarcane is increased while the quality of sugarcane is improved.
Description
Technical field
The present invention relates to the breeding method of a Plants training seedling, relate in particular to a kind of breeding method of sugarcane training seedling.
Background technology
Saccharum asexually propagated crop, traditional planting patterns is to adopt the asexual plantation of reserving seed for planting continuously for many years, this seedling planting technology can make multiple pathogen repeatedly invade, accumulation in pathogen plant, finally can cause original good strains of seeds to be degenerated, particularly the disease such as sugarcane ratoon stunting disease, mosaic disease cannot be very large to the destructiveness of sugarcane plant by the disease of chemicals control, aesthetic infected plant quality and yield constantly declines, to sugarcane grower, cause great economic loss, even threaten the production safety of the whole chain of cane planting industry.
The remarkable effect of the cultivation of the seedling of sugarcane training at present obtains the Ministry of Agriculture, departments at different levels of the Department of Science and Technology and sugarcane advantage producing region and sugar enterprise and certainly approves and pay much attention to, Some Enterprises leader repeatedly investigate virus-elimination seedlings and discuss technology transfer to our unit, develops demonstration.To advancing sugar industry to develop in a healthy way continually and steadily and realizing increasing peasant income, growth of agricultural efficiency and the rural social stability of ethnic mimority area, borderland, build the significant and wide application prospect in new socialist countryside, borderland.
The breeding method of sugarcane training seedling, in the technical remarkable in economical benefits of cane planting, shows as three aspects: first, average yield per mu improves more than 1.0 tons than tradition plantation, and by 300 yuan of ton sugarcane valencys, mu increases income 300 yuan; Secondly, extend perennial root time limit 2-3, can reduce plant development cost; Finally, improve sugarcane containing sugar 0.5%, reduce sugar refinery ton sugar production cost, can effectively improve the market competitiveness of Sugarcane Industry.Therefore, the popularization of the breeding method of sugarcane training seedling, has solved that sugarcane production low energy is produced, the perennial root time limit is short, the high Three Difficult Issues of production cost.
Summary of the invention
The invention provides a kind of breeding method of a kind of sugarcane training seedling that can improve to a great extent sugarcane sugarcane seedling proliferation rate.
The present invention is achieved by the following technical programs:
A breeding method for sugarcane training seedling, is immersed in stem apex after hot water treatment, then cultivates in vitro, and it comprises the following steps:
1) the sugarcane kind stem that is chopped to simple bud kind is processed to 28-40 minute in 48-60 ℃ of hot water;
2) sugarcane kind stem after treatment is carried out to vernalization in incubator, until the sugarcane kind stem in incubator grows new stem apex;
3) from incubator, take out new long stem apex, put into the thimerosal 5-10 minute that sterilizes, the new stem apex after disinfecting is put into and in blake bottle, carries out seal tissue training seedling and cultivate;
4) until the training seedling in blake bottle, grow to after 2-5 centimetre, training seedling is transferred to cultivate in cup and cultivate, after the training seedling in cultivating cup grows into 10-20 centimetre, be transplanted to plantation in field.
Preferably, the temperature in incubator is at 30-40 ℃.
Preferably, while making thimerosal, the former liquid proportional of water and thimerosal is 50:1, and described sterilized stock solution is plant Special sterilizing liquid.
The present invention has following beneficial effect:
The breeding method of sugarcane training seedling of the present invention is the health seedling production technology extensively adopting in the world, and technical method is proper, and technology maturation has obtained good economic benefit; It is very simple that technology implements, and processing ease is convenient to promote; Plantation way to manage does not have too many change, and sugarcane grower is easy to accept; Sugarcane seedling growth vigor is obvious, early growth and quick tiller, extend jointing early, appearance stalwartness, the situation in whole vegetative period is all better than common seedling; The all technical of virus-elimination seedlings is higher than other seedlings, as the parameter index such as diameter, number of productive tiller of Photosynthetic Rate, sugarcane seedling plant height degree, cane stalk; Output obviously increases, and sugar content obviously increases, and improves the quality of sugarcane when having improved sugarcane yield.
Specific embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to the scope that embodiment represents.
Embodiment 1
A breeding method for sugarcane training seedling, is immersed in stem apex after hot water treatment, then cultivates in vitro, and it comprises the following steps:
1) the sugarcane kind stem that is chopped to simple bud kind is processed 40 minutes in 48 ℃ of hot water;
2) sugarcane kind stem after treatment is carried out to vernalization in incubator, until the sugarcane kind stem in incubator grows new stem apex;
3) from incubator, take out new long stem apex, put into thimerosal and sterilize 5 minutes, the new stem apex after disinfecting is put into and in blake bottle, carries out seal tissue training seedling and cultivate;
4) until the training seedling in blake bottle, grow to after 2 centimetres, training seedling is transferred to cultivate in cup and cultivate, after the training seedling in cultivating cup grows into 10 centimetres, be transplanted to plantation in field.
Temperature in described incubator is at 30 ℃.
During described making thimerosal, the former liquid proportional of water and thimerosal is 50:1, and described sterilized stock solution is plant Special sterilizing liquid.
Embodiment 2
A breeding method for sugarcane training seedling, is immersed in stem apex after hot water treatment, then cultivates in vitro, and it comprises the following steps:
1) the sugarcane kind stem that is chopped to simple bud kind is processed 33 minutes in 52 ℃ of hot water;
2) sugarcane kind stem after treatment is carried out to vernalization in incubator, until the sugarcane kind stem in incubator grows new stem apex;
3) from incubator, take out new long stem apex, put into thimerosal and sterilize 7 minutes, the new stem apex after disinfecting is put into and in blake bottle, carries out seal tissue training seedling and cultivate;
4) until the training seedling in blake bottle, grow to after 3 centimetres, training seedling is transferred to cultivate in cup and cultivate, after the training seedling in cultivating cup grows into 15 centimetres, be transplanted to plantation in field.
Temperature in described incubator is at 35 ℃.
During described making thimerosal, the former liquid proportional of water and thimerosal is 50:1, and described sterilized stock solution is plant Special sterilizing liquid.
Embodiment 3
A breeding method for sugarcane training seedling, is immersed in stem apex after hot water treatment, then cultivates in vitro, and it comprises the following steps:
1) the sugarcane kind stem that is chopped to simple bud kind is processed 40 minutes in 60 ℃ of hot water;
2) sugarcane kind stem after treatment is carried out to vernalization in incubator, until the sugarcane kind stem in incubator grows new stem apex;
3) from incubator, take out new long stem apex, put into thimerosal and sterilize 10 minutes, the new stem apex after disinfecting is put into and in blake bottle, carries out seal tissue training seedling and cultivate;
4) until the training seedling in blake bottle, grow to after 5 centimetres, training seedling is transferred to cultivate in cup and cultivate, after the training seedling in cultivating cup grows into 20 centimetres, be transplanted to plantation in field.
Temperature in described incubator is at 40 ℃.
During described making thimerosal, the former liquid proportional of water and thimerosal is 50:1, and described sterilized stock solution is plant Special sterilizing liquid.
Although embodiment of the present invention are open as above, but it is not restricted to listed utilization in specification and embodiment, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other modification, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and example as described herein.
Claims (3)
1. a breeding method for sugarcane training seedling, is immersed in stem apex after hot water treatment, then cultivates in vitro, it is characterized in that, comprises the following steps:
1) the sugarcane kind stem that is chopped to simple bud kind is processed to 28-40 minute in 48-60 ℃ of hot water;
2) sugarcane kind stem after treatment is carried out to vernalization in incubator, until the sugarcane kind stem in incubator grows new stem apex;
3) from incubator, take out new long stem apex, put into the thimerosal 5-10 minute that sterilizes, the new stem apex after disinfecting is put into and in blake bottle, carries out seal tissue training seedling and cultivate;
4) until the training seedling in blake bottle, grow to after 2-5 centimetre, training seedling is transferred to cultivate in cup and cultivate, after the training seedling in cultivating cup grows into 10-20 centimetre, be transplanted to plantation in field.
2. the breeding method of sugarcane training seedling according to claim 1, is characterized in that, the temperature in incubator is at 30-40 ℃.
3. the breeding method of sugarcane training seedling according to claim 1, is characterized in that, while making thimerosal, the former liquid proportional of water and thimerosal is 50:1, and described sterilized stock solution is plant Special sterilizing liquid.
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| CN201310717159.0A CN103733996A (en) | 2013-12-21 | 2013-12-21 | Cultivation method of sugarcane seedlings |
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| CN201310717159.0A CN103733996A (en) | 2013-12-21 | 2013-12-21 | Cultivation method of sugarcane seedlings |
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| CN103733996A true CN103733996A (en) | 2014-04-23 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106879468A (en) * | 2017-03-17 | 2017-06-23 | 广西壮族自治区农业科学院甘蔗研究所 | It is a kind of to improve the method that sugarcane stem apex is trained motility rate |
Citations (7)
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| CN101361458A (en) * | 2008-09-27 | 2009-02-11 | 云南省农业科学院甘蔗研究所 | Efficient breeding method of sugarcane health seedling |
| CN101637167A (en) * | 2009-08-26 | 2010-02-03 | 云南省农业科学院甘蔗研究所 | Special sterilizing and strengthening agent for sugarcane tissue culture seedlings and use method thereof |
| CN101720668A (en) * | 2009-09-18 | 2010-06-09 | 广西壮族自治区农业科学院 | Method for tissue culturing and quick propagation of sugarcane with intermittent immersion bioreactor |
| CN101904262A (en) * | 2010-07-16 | 2010-12-08 | 广西壮族自治区甘蔗研究所 | Ex vitro rooting method of sugarcane test tube plantlets |
| CN102379243A (en) * | 2011-09-14 | 2012-03-21 | 云南省农业科学院甘蔗研究所 | Method of tissue culture and rapid propagation of healthy sugarcane seedlings |
| CN102907328A (en) * | 2012-11-14 | 2013-02-06 | 中国热带农业科学院热带生物技术研究所 | Method for utilizing microelements to accelerate tissue culture seedling of sugarcane |
| CN103168694A (en) * | 2013-04-12 | 2013-06-26 | 广西橡胶研究所 | Method for eliminating ratoon stunting disease |
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2013
- 2013-12-21 CN CN201310717159.0A patent/CN103733996A/en active Pending
Patent Citations (7)
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| CN101361458A (en) * | 2008-09-27 | 2009-02-11 | 云南省农业科学院甘蔗研究所 | Efficient breeding method of sugarcane health seedling |
| CN101637167A (en) * | 2009-08-26 | 2010-02-03 | 云南省农业科学院甘蔗研究所 | Special sterilizing and strengthening agent for sugarcane tissue culture seedlings and use method thereof |
| CN101720668A (en) * | 2009-09-18 | 2010-06-09 | 广西壮族自治区农业科学院 | Method for tissue culturing and quick propagation of sugarcane with intermittent immersion bioreactor |
| CN101904262A (en) * | 2010-07-16 | 2010-12-08 | 广西壮族自治区甘蔗研究所 | Ex vitro rooting method of sugarcane test tube plantlets |
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| CN102907328A (en) * | 2012-11-14 | 2013-02-06 | 中国热带农业科学院热带生物技术研究所 | Method for utilizing microelements to accelerate tissue culture seedling of sugarcane |
| CN103168694A (en) * | 2013-04-12 | 2013-06-26 | 广西橡胶研究所 | Method for eliminating ratoon stunting disease |
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| S. IJAZ,ET AL.: "Establishment of an in vitro regeneration system for genetic transformation of selected sugarcane genotypes", 《GENETICS AND MOLECULAR RESEARCH》 * |
| 周明强等: "甘蔗脱毒种苗培育技术研究", 《贵州农业科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106879468A (en) * | 2017-03-17 | 2017-06-23 | 广西壮族自治区农业科学院甘蔗研究所 | It is a kind of to improve the method that sugarcane stem apex is trained motility rate |
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Application publication date: 20140423 |